Restriction map for
pFN21AE3582
(Circular) (Six-base) MAPSORT of: pFN21AE3582.seq Check: 9820 from: 1 to: 6379
pFN21AE3582 6379 bp
With 182 enzymes: SgfI *
January 27, 2015 16:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2460 4189 278
Size: 53 83 186 936 924 1729 2468
Fragments arranged by size:
2468 1729 936 924 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2343 2744 3722 1937
Size: 406 401 978 4594
Fragments arranged by size:
4594 978 406 401
Acc65I G'GTAC_C
Cuts at: 2052 3700 2052
Size: 1648 4731
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2107 2514 2529 4442 5826 6094 1632
Size: 475 407 15 1913 1384 268 1917
Fragments arranged by size:
1917 1913 1384 475 407 268 15
AflII C'TTAA_G
Cuts at: 820 1017 2516 820
Size: 197 1499 4683
Fragments arranged by size:
4683 1499 197
AflIII A'CryG_T
Cuts at: 1203 2790 5278 6255 1203
Size: 1587 2488 977 1327
Fragments arranged by size:
2488 1587 1327 977
AgeI A'CCGG_T
Cuts at: 2319 5210 2319
Size: 2891 3488
AhdI GACnn_n'nnGTC
Cuts at: 3076 5108 3076
Size: 2032 4347
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2751 3063 1184
Size: 1567 312 4500
Fragments arranged by size:
4500 1567 312
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2015 2047 2240 2272 2594 2626 2015
Size: 32 193 32 322 32 5768
Fragments arranged by size:
5768 322 193 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2041 3037 4122 5694 2041
Size: 996 1085 1572 2726
Fragments arranged by size:
2726 1572 1085 996
ApaLI G'TGCA_C
Cuts at: 4436 5592 4436
Size: 1156 5223
ApoI r'AATT_y
Cuts at: 1493 3688 3914 4066 6175 1493
Size: 2195 226 152 2109 1697
Fragments arranged by size:
2195 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4933 160
Size: 872 3901 1606
Fragments arranged by size:
3901 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 2244 2606 2896 3704 1870
Size: 73 76 225 362 290 808 4545
Fragments arranged by size:
4545 808 362 290 225 76 73
BamHI G'GATC_C
Cuts at: 2707 3709 4076 2707
Size: 1002 367 5010
Fragments arranged by size:
5010 1002 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2052 3197 3700 5155 618
Size: 325 217 627 265 1145 503 1455 1842
Fragments arranged by size:
1842 1455 1145 627 503 325 265 217
BanII G_rGCy'C
Cuts at: 1391 2905 3351 3639 3698 1391
Size: 1514 446 288 59 4072
Fragments arranged by size:
4072 1514 446 288 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4570 4604 1903
Size: 34 2633 34 3678
Fragments arranged by size:
3678 2633 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4271 5481 4271
Size: 1210 5169
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6379
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4990 136
Size: 107 122 71 631 605 3318 1525
Fragments arranged by size:
3318 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3495 6374 3495
Size: 2879 3500
BlpI GC'TnA_GC
Cuts at: 3796 3796
Size: 6379
Bme1580I G_kGCm'C
Cuts at: 1790 4440 5596 1790
Size: 2650 1156 2573
Fragments arranged by size:
2650 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2194 1318
Size: 876 5503
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2023 4110 5068
Size: 416 53 83 399 24 606 2087 958
Cuts at: 5068 442
Size: 1753
Fragments arranged by size:
2087 1753 958 606 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6379
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 6379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2678 2960 3335 5039 1239
Size: 1439 282 375 1704 2579
Fragments arranged by size:
2579 1704 1439 375 282
Bpu10I CC'TnA_GC
Cuts at: 3585 3585
Size: 6379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3401 3588 4504 5369 5667 3401
Size: 187 916 865 298 4113
Fragments arranged by size:
4113 916 865 298 187
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2648 5042 882
Size: 617 1149 2394 2219
Fragments arranged by size:
2394 2219 1149 617
BsaAI yAC'GTr
Cuts at: 493 1204 2305 2866 4170 493
Size: 711 1101 561 1304 2702
Fragments arranged by size:
2702 1304 1101 711 561
BsaBI GATnn'nnATC
Cuts at: 6373 6373
Size: 6379
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2457 2933 4186 4568
Size: 53 83 186 936 924 476 1253 382
Cuts at: 4568 5203 275
Size: 635 1451
Fragments arranged by size:
1451 1253 936 924 635 476 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2319 2412 4812 5210 5484 5631 6042 2319
Size: 93 2400 398 274 147 411 2656
Fragments arranged by size:
2656 2400 411 398 274 147 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2240 2270 3459 3489 1178
Size: 30 165 30 837 30 1189 30 4068
Fragments arranged by size:
4068 1189 837 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3347 1186
Size: 2161 4218
BseYI C'CCAG_C
Cuts at: 2595 2971 3240 3553 5582 6078 2595
Size: 376 269 313 2029 496 2896
Fragments arranged by size:
2896 2029 496 376 313 269
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2285 2846 3023 1163
Size: 573 549 561 177 4519
Fragments arranged by size:
4519 573 561 549 177
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3829 4590 5618 1540
Size: 275 186 1828 761 1028 2301
Fragments arranged by size:
2301 1828 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 3351 3492 3604 4440 4525 5596 2064
Size: 1287 141 112 836 85 1071 2847
Fragments arranged by size:
2847 1287 1071 836 141 112 85
BsmI GAATG_Cn'
Cuts at: 2671 3890 3983 2671
Size: 1219 93 5067
Fragments arranged by size:
5067 1219 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2666 2666
Size: 6379
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2905 3351 3492 3604 3639 3698
Size: 399 274 841 446 141 112 35 59
Cuts at: 3698 4440 4525 5596 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 841 742 446 399 274 141
112 85 59 35
BspEI T'CCGG_A
Cuts at: 2412 6042 2412
Size: 3630 2749
BspHI T'CATG_A
Cuts at: 4268 5998 4268
Size: 1730 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 3045 3734 844
Size: 1244 957 689 3489
Fragments arranged by size:
3489 1244 957 689
BsrBI CCG'CTC
Cuts at: 1394 1823 2012 2067 2251 2418 4266 1394
Size: 429 189 55 184 167 1848 3507
Fragments arranged by size:
3507 1848 429 189 184 167 55
BsrDI GCAATG_nn'
Cuts at: 65 1248 2054 2577 2625 2853 4874 5048 65
Size: 1183 806 523 48 228 2021 174 1396
Fragments arranged by size:
2021 1396 1183 806 523 228 174 48
BsrFI r'CCGG_y
Cuts at: 1700 1810 2319 5023 5210 1700
Size: 110 509 2704 187 2869
Fragments arranged by size:
2869 2704 509 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6379
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6379
BssSI C'ACGA_G
Cuts at: 4439 5451 4439
Size: 1012 5367
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6379
BstBI TT'CG_AA
Cuts at: 5183 5183
Size: 6379
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6379
BstXI CCAn_nnnn'nTGG
Cuts at: 2179 2233 2590 3292 2179
Size: 54 357 702 5266
Fragments arranged by size:
5266 702 357 54
BstYI r'GATC_y
Cuts at: 1972 2707 3273 3495 3709 4076 4461 4478 5930
Size: 735 566 222 214 367 385 17 1452
Cuts at: 5930 6016 6028 6374 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 735 566 385 367 346 222
214 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2146 513
Size: 552 939 142 4746
Fragments arranged by size:
4746 939 552 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5640
Fragments arranged by size:
5640 683 56
BtsI GCAGTG_nn'
Cuts at: 2724 3604 3895 4689 4709 2724
Size: 880 291 794 20 4394
Fragments arranged by size:
4394 880 794 291 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6344
DraI TTT'AAA
Cuts at: 3683 3683
Size: 6379
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5386 809
Size: 507 4070 1802
Fragments arranged by size:
4070 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2217 2700
Size: 54 1425 50 275 18 177 210 483
Cuts at: 2700 2838 3163 3282 3826 4715 6344 8
Size: 138 325 119 544 889 1629 43
Fragments arranged by size:
1629 1425 889 544 483 325 275 210
177 138 119 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3826 1537
Size: 275 2014 4090
Fragments arranged by size:
4090 2014 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 2533 4309 1353
Size: 743 437 1776 3423
Fragments arranged by size:
3423 1776 743 437
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4966 5340 5486 387
Size: 1064 138 3377 374 146 1280
Fragments arranged by size:
3377 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3349 3349
Size: 6379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2107 2514 2529 2678 2960 3335 4442
Size: 393 475 407 15 149 282 375 1107
Cuts at: 4442 5039 5826 6094 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1107 787 597 475 407 393 375
282 268 149 15
EcoO109I rG'GnC_Cy
Cuts at: 2023 2492 2537 3131 3307 3376 2023
Size: 469 45 594 176 69 5026
Fragments arranged by size:
5026 594 469 176 69 45
EcoRI G'AATT_C
Cuts at: 3688 4066 3688
Size: 378 6001
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 6379
FspI TGC'GCA
Cuts at: 4885 4885
Size: 6379
HaeII r_GCGC'y
Cuts at: 1678 5526 1678
Size: 3848 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3062 3094 3459 3491 5020
Size: 32 135 32 1490 32 365 32 1529
Cuts at: 5020 5052 5094 5126 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1529 1490 365 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2371 3723 3969 669
Size: 988 281 433 1352 246 3079
Fragments arranged by size:
3079 1352 988 433 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6379
HpaI GTT'AAC
Cuts at: 3969 3969
Size: 6379
KpnI G_GTAC'C
Cuts at: 2056 3704 2056
Size: 1648 4731
MfeI C'AATT_G
Cuts at: 3978 3978
Size: 6379
MluI A'CGCG_T
Cuts at: 6255 6255
Size: 6379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2508 2583 5493 5677 1305
Size: 1203 75 2910 184 2007
Fragments arranged by size:
2910 2007 1203 184 75
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2219 3165 3284 10
Size: 54 1425 343 387 946 119 3105
Fragments arranged by size:
3105 1425 946 387 343 119 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3203 4337 4696 4855 518
Size: 648 2037 1134 359 159 2042
Fragments arranged by size:
2042 2037 1134 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2599 2890 3565 3795 4472 5620 5865 1606
Size: 993 291 675 230 677 1148 245 2120
Fragments arranged by size:
2120 1148 993 677 675 291 245 230
NcoI C'CATG_G
Cuts at: 513 1065 2004 2146 513
Size: 552 939 142 4746
Fragments arranged by size:
4746 939 552 142
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6379
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6379
NotI GC'GGCC_GC
Cuts at: 3826 3826
Size: 6379
NspI r_CATG'y
Cuts at: 1132 1229 2794 3173 3737 5282 1132
Size: 97 1565 379 564 1545 2229
Fragments arranged by size:
2229 1565 1545 564 379 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6379
PciI A'CATG_T
Cuts at: 2790 5278 2790
Size: 2488 3891
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3141 1108
Size: 2033 4346
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PmlI CAC'GTG
Cuts at: 2305 2866 2305
Size: 561 5818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2240 2272 4448 4480 5961 5993 2240
Size: 32 2176 32 1481 32 2626
Fragments arranged by size:
2626 2176 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2023 2492 2537 3376 2023
Size: 469 45 839 5026
Fragments arranged by size:
5026 839 469 45
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6379
PsiI TTA'TAA
Cuts at: 3949 3949
Size: 6379
PstI C_TGCA'G
Cuts at: 830 2046 2719 3731 830
Size: 1216 673 1012 3478
Fragments arranged by size:
3478 1216 1012 673
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6379
PvuII CAG'CTG
Cuts at: 2599 2890 3565 2599
Size: 291 675 5413
Fragments arranged by size:
5413 675 291
SacI G_AGCT'C
Cuts at: 3351 3351
Size: 6379
SalI G'TCGA_C
Cuts at: 1936 3721 1936
Size: 1785 4594
SanDI GG'GwC_CC
Cuts at: 2023 2492 2023
Size: 469 5910
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 4627 6339 4627
Size: 1712 4667
SexAI A'CCwGG_T
Cuts at: 2178 2178
Size: 6379
SfcI C'TryA_G
Cuts at: 826 1045 2042 2715 3727 4862 5543 5734 826
Size: 219 997 673 1012 1135 681 191 1471
Fragments arranged by size:
1471 1135 1012 997 681 673 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6379
SmaI CCC'GGG
Cuts at: 1872 2021 2246 3706 1872
Size: 149 225 1460 4545
Fragments arranged by size:
4545 1460 225 149
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2516 3380 3603 4483 5384 5646
Size: 197 926 573 864 223 880 901 262
Cuts at: 5646 820
Size: 1553
Fragments arranged by size:
1553 926 901 880 864 573 262 223
197
SnaBI TAC'GTA
Cuts at: 493 4170 493
Size: 3677 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6379
SphI G_CATG'C
Cuts at: 1132 3173 3737 1132
Size: 2041 564 3774
Fragments arranged by size:
3774 2041 564
SspI AAT'ATT
Cuts at: 5 52 4303 5
Size: 47 4251 2081
Fragments arranged by size:
4251 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6379
StyI C'CwwG_G
Cuts at: 513 1065 2004 2146 2703 3193 3471 3818 513
Size: 552 939 142 557 490 278 347 3074
Fragments arranged by size:
3074 939 557 552 490 347 278 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2347 2800 2988 4406 4423 4576 4761
Size: 1255 767 453 188 1418 17 153 185
Cuts at: 4761 5206 325
Size: 445 1498
Fragments arranged by size:
1498 1418 1255 767 453 445 188 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4625 6337 96
Size: 274 80 33 51 1443 2648 1712 138
Fragments arranged by size:
2648 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3099 3131 6339 6371 3099
Size: 32 3208 32 3107
Fragments arranged by size:
3208 3107 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6379
XbaI T'CTAG_A
Cuts at: 3715 3715
Size: 6379
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6379
XmaI C'CCGG_G
Cuts at: 1870 2019 2244 3704 1870
Size: 149 225 1460 4545
Fragments arranged by size:
4545 1460 225 149
XmnI GAAnn'nnTTC
Cuts at: 2396 4508 2396
Size: 2112 4267
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2458 4187 276
Size: 53 83 186 936 924 1729 2468
Fragments arranged by size:
2468 1729 936 924 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PspOMI PspXI PsrI RsrII SacII SapI SfiI
SfoI SrfI SwaI XcmI