Restriction map for
pFN21AE3561
(Circular) (Six-base) MAPSORT of: pFN21AE3561.seq Check: 2943 from: 1 to: 7483
pFN21AE3561 7483 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5293 278
Size: 53 83 186 936 3757 2468
Fragments arranged by size:
3757 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4826 1937
Size: 2889 4594
Acc65I G'GTAC_C
Cuts at: 4804 4804
Size: 7483
AclI AA'CG_TT
Cuts at: 1563 5610 5983 1563
Size: 4047 373 3063
Fragments arranged by size:
4047 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2721 2810 4118 4682 5546 6930 7198 1632
Size: 1089 89 1308 564 864 1384 268 1917
Fragments arranged by size:
1917 1384 1308 1089 864 564 268 89
AflII C'TTAA_G
Cuts at: 820 1017 3548 820
Size: 197 2531 4755
Fragments arranged by size:
4755 2531 197
AflIII A'CryG_T
Cuts at: 1203 2712 3977 6382 7359 1203
Size: 1509 1265 2405 977 1327
Fragments arranged by size:
2405 1509 1327 1265 977
AgeI A'CCGG_T
Cuts at: 6314 6314
Size: 7483
AhdI GACnn_n'nnGTC
Cuts at: 6212 6212
Size: 7483
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3105 4738 1184
Size: 1921 1633 3929
Fragments arranged by size:
3929 1921 1633
AlwNI CAG_nnn'CTG
Cuts at: 5226 6798 5226
Size: 1572 5911
ApaI G_GGCC'C
Cuts at: 3721 3721
Size: 7483
ApaLI G'TGCA_C
Cuts at: 5540 6696 5540
Size: 1156 6327
ApoI r'AATT_y
Cuts at: 1493 2360 2482 2541 3382 3555 4053 4091 4349
Size: 867 122 59 841 173 498 38 258
Cuts at: 4349 4792 5018 5170 7279 1493
Size: 443 226 152 2109 1697
Fragments arranged by size:
2109 1697 867 841 498 443 258 226
173 152 122 59 38
AseI AT'TA_AT
Cuts at: 160 1032 4460 6037 160
Size: 872 3428 1577 1606
Fragments arranged by size:
3428 1606 1577 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7483
AvaI C'yCGr_G
Cuts at: 1870 1943 4808 1870
Size: 73 2865 4545
Fragments arranged by size:
4545 2865 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4728 4761 4728
Size: 33 7450
BamHI G'GATC_C
Cuts at: 4244 4813 5180 4244
Size: 569 367 6547
Fragments arranged by size:
6547 569 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4262 4804 6259 618
Size: 325 217 627 2475 542 1455 1842
Fragments arranged by size:
2475 1842 1455 627 542 325 217
BanII G_rGCy'C
Cuts at: 1391 3721 4802 1391
Size: 2330 1081 4072
Fragments arranged by size:
4072 2330 1081
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2166 3496 928
Size: 971 267 1330 4915
Fragments arranged by size:
4915 1330 971 267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5674 5708 1903
Size: 34 3737 34 3678
Fragments arranged by size:
3737 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5375 6585 5375
Size: 1210 6273
BclI T'GATC_A
Cuts at: 1548 2253 1548
Size: 705 6778
BfrBI ATG'CAT
Cuts at: 3082 3129 3082
Size: 47 7436
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3164 4289 6094
Size: 107 122 71 631 605 1492 1125 1805
Cuts at: 6094 136
Size: 1525
Fragments arranged by size:
1805 1525 1492 1125 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4693 7478 4693
Size: 2785 4698
BlpI GC'TnA_GC
Cuts at: 3225 4900 3225
Size: 1675 5808
Bme1580I G_kGCm'C
Cuts at: 1790 2574 3721 5544 6700 1790
Size: 784 1147 1823 1156 2573
Fragments arranged by size:
2573 1823 1156 1147 784
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7483
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2297 2568 3039
Size: 416 53 83 399 24 880 271 471
Cuts at: 3039 3724 3821 5214 6172 442
Size: 685 97 1393 958 1753
Fragments arranged by size:
1753 1393 958 880 685 471 416 399
271 97 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4064 1056
Size: 3008 4475
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4032 4032
Size: 7483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2298 2356 2455 4250 6143 1239
Size: 1059 58 99 1795 1893 2579
Fragments arranged by size:
2579 1893 1795 1059 99 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4538 5608 6473 6771 4538
Size: 1070 865 298 5250
Fragments arranged by size:
5250 1070 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6146 882
Size: 617 4647 2219
Fragments arranged by size:
4647 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5274 493
Size: 711 4070 2702
Fragments arranged by size:
4070 2702 711
BsaBI GATnn'nnATC
Cuts at: 2639 7477 2639
Size: 4838 2645
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5290 5672 6307 275
Size: 53 83 186 936 3757 382 635 1451
Fragments arranged by size:
3757 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5916 6314 6588 6735 7146 5916
Size: 398 274 147 411 6253
Fragments arranged by size:
6253 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2378 2408 1178
Size: 30 165 30 975 30 6253
Fragments arranged by size:
6253 975 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4253 1186
Size: 3067 4416
BseYI C'CCAG_C
Cuts at: 4270 4652 6686 7182 4270
Size: 382 2034 496 4571
Fragments arranged by size:
4571 2034 496 382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3017 1163
Size: 573 1281 5629
Fragments arranged by size:
5629 1281 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4933 5694 6722 1540
Size: 275 186 2932 761 1028 2301
Fragments arranged by size:
2932 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3438 3734 5544 5629 6700 3438
Size: 296 1810 85 1071 4221
Fragments arranged by size:
4221 1810 1071 296 85
BsmI GAATG_Cn'
Cuts at: 2009 3584 4994 5087 2009
Size: 1575 1410 93 4405
Fragments arranged by size:
4405 1575 1410 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2574 3438 3721 3734 4802 5544 5629
Size: 399 784 864 283 13 1068 742 85
Cuts at: 5629 6700 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1068 864 784 742 399 283
85 13
BspEI T'CCGG_A
Cuts at: 7146 7146
Size: 7483
BspHI T'CATG_A
Cuts at: 2137 3195 5372 7102 2137
Size: 1058 2177 1730 2518
Fragments arranged by size:
2518 2177 1730 1058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2039 4838 844
Size: 1195 2799 3489
Fragments arranged by size:
3489 2799 1195
BsrBI CCG'CTC
Cuts at: 1394 1823 4629 5370 1394
Size: 429 2806 741 3507
Fragments arranged by size:
3507 2806 741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2337 2604 4278 4593 4639 5978 6152
Size: 1183 1089 267 1674 315 46 1339 174
Cuts at: 6152 65
Size: 1396
Fragments arranged by size:
1674 1396 1339 1183 1089 315 267 174
46
BsrFI r'CCGG_y
Cuts at: 1700 1810 4435 6127 6314 1700
Size: 110 2625 1692 187 2869
Fragments arranged by size:
2869 2625 1692 187 110
BsrGI T'GTAC_A
Cuts at: 96 2312 96
Size: 2216 5267
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7483
BssSI C'ACGA_G
Cuts at: 3300 3943 5543 6555 3300
Size: 643 1600 1012 4228
Fragments arranged by size:
4228 1600 1012 643
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3263 3738 4029 794
Size: 2469 475 291 4248
Fragments arranged by size:
4248 2469 475 291
BstBI TT'CG_AA
Cuts at: 6287 6287
Size: 7483
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7483
BstYI r'GATC_y
Cuts at: 1972 3286 4244 4693 4813 5180 5565 5582 7034
Size: 1314 958 449 120 367 385 17 1452
Cuts at: 7034 7120 7132 7478 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1314 958 449 385 367 346
120 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3332 513
Size: 552 939 1328 4664
Fragments arranged by size:
4664 1328 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6744
Fragments arranged by size:
6744 683 56
BtsI GCAGTG_nn'
Cuts at: 3264 3685 4999 5793 5813 3264
Size: 421 1314 794 20 4934
Fragments arranged by size:
4934 1314 794 421 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7483
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7448
DraI TTT'AAA
Cuts at: 3414 4787 3414
Size: 1373 6110
DraIII CAC_nnn'GTG
Cuts at: 3738 3738
Size: 7483
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6490 809
Size: 507 5174 1802
Fragments arranged by size:
5174 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3783 4930 5819
Size: 54 1425 50 275 18 1953 1147 889
Cuts at: 5819 7448 8
Size: 1629 43
Fragments arranged by size:
1953 1629 1425 1147 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4930 1537
Size: 275 3118 4090
Fragments arranged by size:
4090 3118 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2867 2986 5413 1353
Size: 1514 119 2427 3423
Fragments arranged by size:
3423 2427 1514 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6070 6444 6590 387
Size: 1064 138 4481 374 146 1280
Fragments arranged by size:
4481 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2298 2356 2455 2721 2810 4118 4250
Size: 393 666 58 99 266 89 1308 132
Cuts at: 4250 4682 5546 6143 6930 7198 1239
Size: 432 864 597 787 268 1524
Fragments arranged by size:
1524 1308 864 787 666 597 432 393
268 266 132 99 89 58
EcoO109I rG'GnC_Cy
Cuts at: 3718 3718
Size: 7483
EcoRI G'AATT_C
Cuts at: 3382 4792 5170 3382
Size: 1410 378 5695
Fragments arranged by size:
5695 1410 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2753 4564 2753
Size: 1811 5672
FspI TGC'GCA
Cuts at: 3097 5989 3097
Size: 2892 4591
HaeII r_GCGC'y
Cuts at: 1678 6630 1678
Size: 4952 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2166 2198 2903 2935 3882
Size: 32 135 32 594 32 705 32 947
Cuts at: 3882 3914 4030 4062 4209 4229 4241 4261 6124
Size: 32 116 32 147 20 12 20 1863
Cuts at: 6124 6156 6198 6230 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1863 947 705 594 147 135 116
42 32 32 32 32 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2922 3617 4827 5073 669
Size: 988 281 984 695 1210 246 3079
Fragments arranged by size:
3079 1210 988 984 695 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7483
HpaI GTT'AAC
Cuts at: 5073 5073
Size: 7483
KpnI G_GTAC'C
Cuts at: 4808 4808
Size: 7483
MfeI C'AATT_G
Cuts at: 5082 5082
Size: 7483
MluI A'CGCG_T
Cuts at: 7359 7359
Size: 7483
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2606 6597 6781 1305
Size: 1301 3991 184 2007
Fragments arranged by size:
3991 2007 1301 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3785 10
Size: 54 1425 343 1953 3708
Fragments arranged by size:
3708 1953 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 2879 3072 3396 3473 4720 5441
Size: 648 1083 630 193 324 77 1247 721
Cuts at: 5441 5800 5959 518
Size: 359 159 2042
Fragments arranged by size:
2042 1247 1083 721 648 630 359 324
193 159 77
MspA1I CmG'CkG
Cuts at: 1606 4899 5576 6724 6969 1606
Size: 3293 677 1148 245 2120
Fragments arranged by size:
3293 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3332 513
Size: 552 939 1328 4664
Fragments arranged by size:
4664 1328 939 552
NdeI CA'TA_TG
Cuts at: 387 3100 4721 387
Size: 2713 1621 3149
Fragments arranged by size:
3149 2713 1621
NheI G'CTAG_C
Cuts at: 1052 4060 1052
Size: 3008 4475
NotI GC'GGCC_GC
Cuts at: 4930 4930
Size: 7483
NsiI A_TGCA'T
Cuts at: 3084 3131 3084
Size: 47 7436
NspI r_CATG'y
Cuts at: 1132 1229 2416 3981 4369 4841 6386 1132
Size: 97 1187 1565 388 472 1545 2229
Fragments arranged by size:
2229 1565 1545 1187 472 388 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7483
PciI A'CATG_T
Cuts at: 3977 6382 3977
Size: 2405 5078
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7483
PfoI T'CCnGG_A
Cuts at: 2101 2184 2471 4227 2101
Size: 83 287 1756 5357
Fragments arranged by size:
5357 1756 287 83
PmeI GTTT'AAAC
Cuts at: 4787 4787
Size: 7483
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5552 5584 7065 7097 5552
Size: 32 1481 32 5938
Fragments arranged by size:
5938 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7483
PsiI TTA'TAA
Cuts at: 2690 5053 2690
Size: 2363 5120
PspOMI G'GGCC_C
Cuts at: 3717 3717
Size: 7483
PstI C_TGCA'G
Cuts at: 830 2524 4835 830
Size: 1694 2311 3478
Fragments arranged by size:
3478 2311 1694
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7483
SalI G'TCGA_C
Cuts at: 1936 4825 1936
Size: 2889 4594
SbfI CC_TGCA'GG
Cuts at: 4835 4835
Size: 7483
ScaI AGT'ACT
Cuts at: 5731 7443 5731
Size: 1712 5771
SfcI C'TryA_G
Cuts at: 826 1045 2520 3177 3491 4108 4191 4564 4831
Size: 219 1475 657 314 617 83 373 267
Cuts at: 4831 5966 6647 6838 826
Size: 1135 681 191 1471
Fragments arranged by size:
1475 1471 1135 681 657 617 373 314
267 219 191 83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7483
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7483
SmaI CCC'GGG
Cuts at: 1872 4810 1872
Size: 2938 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3548 4553 5587 6488 6750 820
Size: 197 926 1605 1005 1034 901 262 1553
Fragments arranged by size:
1605 1553 1034 1005 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5274 493
Size: 4781 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7483
SphI G_CATG'C
Cuts at: 1132 4369 4841 1132
Size: 3237 472 3774
Fragments arranged by size:
3774 3237 472
SspI AAT'ATT
Cuts at: 5 52 3846 5407 5
Size: 47 3794 1561 2081
Fragments arranged by size:
3794 2081 1561 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7483
StyI C'CwwG_G
Cuts at: 513 1065 2004 3268 3332 3573 3780 4922 513
Size: 552 939 1264 64 241 207 1142 3074
Fragments arranged by size:
3074 1264 1142 939 552 241 207 64
SwaI ATTT'AAAT
Cuts at: 3414 3414
Size: 7483
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4129 5510 5527 5680 5865 6310 325
Size: 1255 2549 1381 17 153 185 445 1498
Fragments arranged by size:
2549 1498 1381 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2312 5729 7441
Size: 274 80 33 51 1443 335 3417 1712
Cuts at: 7441 96
Size: 138
Fragments arranged by size:
3417 1712 1443 335 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2378 2410 2893 2925 7443 7475 2378
Size: 32 483 32 4518 32 2386
Fragments arranged by size:
4518 2386 483 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4042 1316
Size: 2726 4757
XbaI T'CTAG_A
Cuts at: 4819 4819
Size: 7483
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2192 2380 2192
Size: 188 7295
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7483
XmaI C'CCGG_G
Cuts at: 1870 4808 1870
Size: 2938 4545
XmnI GAAnn'nnTTC
Cuts at: 5612 5612
Size: 7483
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5291 276
Size: 53 83 186 936 3757 2468
Fragments arranged by size:
3757 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI