Restriction map for
pFN21AE3557
(Circular) (Six-base) MAPSORT of: pFN21AE3557.seq Check: 6442 from: 1 to: 6349
pFN21AE3557 6349 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3273 4159 278
Size: 53 83 186 936 1737 886 2468
Fragments arranged by size:
2468 1737 936 886 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3692 1937
Size: 1755 4594
Acc65I G'GTAC_C
Cuts at: 3670 3670
Size: 6349
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3314 4412 5796 6064 1632
Size: 1682 1098 1384 268 1917
Fragments arranged by size:
1917 1682 1384 1098 268
AfeI AGC'GCT
Cuts at: 2885 2885
Size: 6349
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 5248 6225 1203
Size: 4045 977 1327
Fragments arranged by size:
4045 1327 977
AgeI A'CCGG_T
Cuts at: 5180 5180
Size: 6349
AhdI GACnn_n'nnGTC
Cuts at: 2872 5078 2872
Size: 2206 4143
AleI CACnn'nnGTG
Cuts at: 1166 2541 1166
Size: 1375 4974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2559 3524 1184
Size: 1375 965 4009
Fragments arranged by size:
4009 1375 965
AlwNI CAG_nnn'CTG
Cuts at: 2453 2787 2821 3031 3396 4092 5664 2453
Size: 334 34 210 365 696 1572 3138
Fragments arranged by size:
3138 1572 696 365 334 210 34
ApaI G_GGCC'C
Cuts at: 2689 2951 3537 2689
Size: 262 586 5501
Fragments arranged by size:
5501 586 262
ApaLI G'TGCA_C
Cuts at: 4406 5562 4406
Size: 1156 5193
ApoI r'AATT_y
Cuts at: 1493 3658 3884 4036 6145 1493
Size: 2165 226 152 2109 1697
Fragments arranged by size:
2165 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4903 160
Size: 872 3871 1606
Fragments arranged by size:
3871 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2072 2093 3317 3551 3674 1870
Size: 73 114 15 21 1224 234 123 4545
Fragments arranged by size:
4545 1224 234 123 114 73 21 15
BamHI G'GATC_C
Cuts at: 3679 4046 3679
Size: 367 5982
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3109 3670 5125 618
Size: 325 217 627 1322 561 1455 1842
Fragments arranged by size:
1842 1455 1322 627 561 325 217
BanII G_rGCy'C
Cuts at: 1391 2024 2120 2133 2689 2951 3292 3324 3537
Size: 633 96 13 556 262 341 32 213
Cuts at: 3537 3612 3668 1391
Size: 75 56 4072
Fragments arranged by size:
4072 633 556 341 262 213 96 75
56 32 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5378
BbvCI CC'TCA_GC
Cuts at: 2663 2663
Size: 6349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4540 4574 1903
Size: 34 50 34 2519 34 3678
Fragments arranged by size:
3678 2519 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6349
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2739 2955 3146
Size: 107 122 71 631 605 1067 216 191
Cuts at: 3146 4960 136
Size: 1814 1525
Fragments arranged by size:
1814 1525 1067 631 605 216 191 122
107 71
BglII A'GATC_T
Cuts at: 6344 6344
Size: 6349
BlpI GC'TnA_GC
Cuts at: 3766 3766
Size: 6349
Bme1580I G_kGCm'C
Cuts at: 1790 2689 2951 3537 4410 5566 1790
Size: 899 262 586 873 1156 2573
Fragments arranged by size:
2573 1156 899 873 586 262
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6349
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4080 5038 442
Size: 416 53 83 399 24 2663 958 1753
Fragments arranged by size:
2663 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6349
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2302 2452 2302
Size: 150 6199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2367 2560 2582 2741 2981 2990 5009
Size: 1022 106 193 22 159 240 9 2019
Cuts at: 5009 1239
Size: 2579
Fragments arranged by size:
2579 2019 1022 240 193 159 106 22
9
Bpu10I CC'TnA_GC
Cuts at: 2018 2663 2805 2018
Size: 645 142 5562
Fragments arranged by size:
5562 645 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2992 4474 5339 5637 2992
Size: 1482 865 298 3704
Fragments arranged by size:
3704 1482 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3557 5012 882
Size: 617 2058 1455 2219
Fragments arranged by size:
2219 2058 1455 617
BsaAI yAC'GTr
Cuts at: 493 1204 4140 493
Size: 711 2936 2702
Fragments arranged by size:
2936 2702 711
BsaBI GATnn'nnATC
Cuts at: 6343 6343
Size: 6349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2571 3270 4156 4538
Size: 53 83 186 936 1038 699 886 382
Cuts at: 4538 5173 275
Size: 635 1451
Fragments arranged by size:
1451 1038 936 886 699 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2526 3284 4782 5180 5454 5601 6012 2526
Size: 758 1498 398 274 147 411 2863
Fragments arranged by size:
2863 1498 758 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6124
Fragments arranged by size:
6124 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2275 2402 2858 2924 3115 1186
Size: 925 164 127 456 66 191 4420
Fragments arranged by size:
4420 925 456 191 164 127 66
BseYI C'CCAG_C
Cuts at: 2365 2398 2424 3261 5552 6048 2365
Size: 33 26 837 2291 496 2666
Fragments arranged by size:
2666 2291 837 496 33 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3143 3530 1163
Size: 573 1407 387 3982
Fragments arranged by size:
3982 1407 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2924 3799 4560 5588 1540
Size: 275 186 923 875 761 1028 2301
Fragments arranged by size:
2301 1028 923 875 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2490 3486 3612 4410 4495 5566 2490
Size: 996 126 798 85 1071 3273
Fragments arranged by size:
3273 1071 996 798 126 85
BsmI GAATG_Cn'
Cuts at: 2932 3860 3953 2932
Size: 928 93 5328
Fragments arranged by size:
5328 928 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2054 2054
Size: 6349
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2120 2133 2490 2689 2951 3292
Size: 399 234 96 13 357 199 262 341
Cuts at: 3292 3324 3486 3537 3612 3668 4410 4495 5566
Size: 32 162 51 75 56 742 85 1071
Cuts at: 5566 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 357 341 262 234
199 162 96 85 75 56 51 32
13
BspEI T'CCGG_A
Cuts at: 2526 6012 2526
Size: 3486 2863
BspHI T'CATG_A
Cuts at: 4238 5968 4238
Size: 1730 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3704 844
Size: 2860 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3428 4236 1394
Size: 429 1605 808 3507
Fragments arranged by size:
3507 1605 808 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2799 3354 4844 5018 65
Size: 1183 1551 555 1490 174 1396
Fragments arranged by size:
1551 1490 1396 1183 555 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4993 5180 1700
Size: 110 3183 187 2869
Fragments arranged by size:
3183 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6349
BssHII G'CGCG_C
Cuts at: 1925 3072 1925
Size: 1147 5202
BssSI C'ACGA_G
Cuts at: 4409 5421 4409
Size: 1012 5337
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3031 794
Size: 2237 4112
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6349
BstXI CCAn_nnnn'nTGG
Cuts at: 2470 3583 2470
Size: 1113 5236
BstYI r'GATC_y
Cuts at: 1972 3679 4046 4431 4448 5900 5986 5998 6344
Size: 1707 367 385 17 1452 86 12 346
Cuts at: 6344 1972
Size: 1977
Fragments arranged by size:
1977 1707 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2618 2618
Size: 6349
BtgI C'CryG_G
Cuts at: 513 1065 2042 2112 2251 2952 3151 3582 513
Size: 552 977 70 139 701 199 431 3280
Fragments arranged by size:
3280 977 701 552 431 199 139 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2477 487
Size: 683 56 1251 4359
Fragments arranged by size:
4359 1251 683 56
BtsI GCAGTG_nn'
Cuts at: 2234 3020 3134 3629 3865 4659 4679 2234
Size: 786 114 495 236 794 20 3904
Fragments arranged by size:
3904 794 786 495 236 114 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6314
DraI TTT'AAA
Cuts at: 3653 3653
Size: 6349
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5356 809
Size: 507 4040 1802
Fragments arranged by size:
4040 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3078 3195 3360
Size: 54 1425 50 275 18 1248 117 165
Cuts at: 3360 3519 3796 4685 6314 8
Size: 159 277 889 1629 43
Fragments arranged by size:
1629 1425 1248 889 277 275 165 159
117 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3796 1537
Size: 275 1984 4090
Fragments arranged by size:
4090 1984 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3305 3332 3461 3555 4279 1353
Size: 1952 27 129 94 724 3423
Fragments arranged by size:
3423 1952 724 129 94 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3335 4936 5310 5456 387
Size: 1064 138 1746 1601 374 146 1280
Fragments arranged by size:
1746 1601 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3610 3610
Size: 6349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2367 2560 2582 2741 2981 2990
Size: 393 629 106 193 22 159 240 9
Cuts at: 2990 3314 4412 5009 5796 6064 1239
Size: 324 1098 597 787 268 1524
Fragments arranged by size:
1524 1098 787 629 597 393 324 268
240 193 159 106 22 9
EcoO109I rG'GnC_Cy
Cuts at: 2038 2245 2311 2395 2686 2948 2038
Size: 207 66 84 291 262 5439
Fragments arranged by size:
5439 291 262 207 84 66
EcoRI G'AATT_C
Cuts at: 3658 4036 3658
Size: 378 5971
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2281 3319 2281
Size: 1038 5311
FspI TGC'GCA
Cuts at: 4855 4855
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 2887 3241 3262 5496 1678
Size: 1209 354 21 2234 2531
Fragments arranged by size:
2531 2234 1209 354 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2858 2890 4990 5022 5064
Size: 32 135 32 1286 32 2100 32 42
Cuts at: 5064 5096 1373
Size: 32 2626
Fragments arranged by size:
2626 2100 1286 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3693 3939 669
Size: 988 281 1755 246 3079
Fragments arranged by size:
3079 1755 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6349
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KpnI G_GTAC'C
Cuts at: 3674 3674
Size: 6349
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5463 5647 1305
Size: 4158 184 2007
Fragments arranged by size:
4158 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3197 3521 10
Size: 54 1425 343 1365 324 2838
Fragments arranged by size:
2838 1425 1365 343 324 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2541 3506 3575 3581 4307 4666 4825
Size: 648 1375 965 69 6 726 359 159
Cuts at: 4825 518
Size: 2042
Fragments arranged by size:
2042 1375 965 726 648 359 159 69
6
MspA1I CmG'CkG
Cuts at: 1606 2044 2450 3153 3396 3765 4442 5590 5835
Size: 438 406 703 243 369 677 1148 245
Cuts at: 5835 1606
Size: 2120
Fragments arranged by size:
2120 1148 703 677 438 406 369 245
243
NcoI C'CATG_G
Cuts at: 513 1065 3582 513
Size: 552 2517 3280
Fragments arranged by size:
3280 2517 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6349
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6349
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 3505 3574 3707 5252 1132
Size: 97 2276 69 133 1545 2229
Fragments arranged by size:
2276 2229 1545 133 97 69
PasI CC'CwG_GG
Cuts at: 1348 2033 2944 1348
Size: 685 911 4753
Fragments arranged by size:
4753 911 685
PciI A'CATG_T
Cuts at: 5248 5248
Size: 6349
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3604 1108
Size: 2496 3853
PfoI T'CCnGG_A
Cuts at: 2549 2814 2549
Size: 265 6084
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2038 2245 2311 2395 2038
Size: 207 66 84 5992
Fragments arranged by size:
5992 207 84 66
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6349
PsiI TTA'TAA
Cuts at: 3919 3919
Size: 6349
PspOMI G'GGCC_C
Cuts at: 2685 2947 3533 2685
Size: 262 586 5501
Fragments arranged by size:
5501 586 262
PstI C_TGCA'G
Cuts at: 830 2611 3027 3621 3701 830
Size: 1781 416 594 80 3478
Fragments arranged by size:
3478 1781 594 416 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
PvuII CAG'CTG
Cuts at: 2450 2450
Size: 6349
SacI G_AGCT'C
Cuts at: 3612 3612
Size: 6349
SacII CC_GC'GG
Cuts at: 2045 3154 2045
Size: 1109 5240
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SanDI GG'GwC_CC
Cuts at: 2038 2038
Size: 6349
SapI GCTCTTCn'nnn_
Cuts at: 3305 3461 3305
Size: 156 6193
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 4597 6309 4597
Size: 1712 4637
SexAI A'CCwGG_T
Cuts at: 3057 3597 3057
Size: 540 5809
SfcI C'TryA_G
Cuts at: 826 1045 2607 3023 3617 3697 4832 5513 5704
Size: 219 1562 416 594 80 1135 681 191
Cuts at: 5704 826
Size: 1471
Fragments arranged by size:
1562 1471 1135 681 594 416 219 191
80
SfiI GGCCn_nnn'nGGCC
Cuts at: 2955 2955
Size: 6349
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6349
SmaI CCC'GGG
Cuts at: 1872 3676 1872
Size: 1804 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2971 4453 5354 5616 820
Size: 197 926 1028 1482 901 262 1553
Fragments arranged by size:
1553 1482 1028 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 3574 3707 1132
Size: 2442 133 3774
Fragments arranged by size:
3774 2442 133
SspI AAT'ATT
Cuts at: 5 52 4273 5
Size: 47 4221 2081
Fragments arranged by size:
4221 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6349
StyI C'CwwG_G
Cuts at: 513 1065 2469 3582 3788 513
Size: 552 1404 1113 206 3074
Fragments arranged by size:
3074 1404 1113 552 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2221 2938 4376 4393 4546 4731 5176
Size: 1255 641 717 1438 17 153 185 445
Cuts at: 5176 325
Size: 1498
Fragments arranged by size:
1498 1438 1255 717 641 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2649 4595 6307
Size: 274 80 33 51 1443 672 1946 1712
Cuts at: 6307 96
Size: 138
Fragments arranged by size:
1946 1712 1443 672 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6309 6341 6309
Size: 32 6317
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6349
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6349
XmaI C'CCGG_G
Cuts at: 1870 3674 1870
Size: 1804 4545
XmnI GAAnn'nnTTC
Cuts at: 3299 4478 3299
Size: 1179 5170
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3271 4157 276
Size: 53 83 186 936 1737 886 2468
Fragments arranged by size:
2468 1737 936 886 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SfoI SrfI SwaI XcmI