Restriction map for
pFN21AE3540
(Circular) (Six-base) MAPSORT of: pFN21AE3540.seq Check: 2057 from: 1 to: 5611
pFN21AE3540 5611 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2101 2803 2101
Size: 702 4909
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3421 278
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2954 1937
Size: 1017 4594
Acc65I G'GTAC_C
Cuts at: 2932 2932
Size: 5611
AclI AA'CG_TT
Cuts at: 1563 3738 4111 1563
Size: 2175 373 3063
Fragments arranged by size:
3063 2175 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2852 3674 5058 5326 1632
Size: 1220 822 1384 268 1917
Fragments arranged by size:
1917 1384 1220 822 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5414
AflIII A'CryG_T
Cuts at: 1203 2458 4510 5487 1203
Size: 1255 2052 977 1327
Fragments arranged by size:
2052 1327 1255 977
AgeI A'CCGG_T
Cuts at: 4442 4442
Size: 5611
AhdI GACnn_n'nnGTC
Cuts at: 4340 4340
Size: 5611
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5611
AlwNI CAG_nnn'CTG
Cuts at: 2101 2638 2683 2800 3354 4926 2101
Size: 537 45 117 554 1572 2786
Fragments arranged by size:
2786 1572 554 537 117 45
ApaI G_GGCC'C
Cuts at: 2543 2543
Size: 5611
ApaLI G'TGCA_C
Cuts at: 2215 2852 3668 4824 2215
Size: 637 816 1156 3002
Fragments arranged by size:
3002 1156 816 637
ApoI r'AATT_y
Cuts at: 1493 2920 3146 3298 5407 1493
Size: 1427 226 152 2109 1697
Fragments arranged by size:
2109 1697 1427 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4165 160
Size: 872 3133 1606
Fragments arranged by size:
3133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
AvaI C'yCGr_G
Cuts at: 1870 1943 2767 2876 2936 1870
Size: 73 824 109 60 4545
Fragments arranged by size:
4545 824 109 73 60
BamHI G'GATC_C
Cuts at: 2941 3308 2941
Size: 367 5244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2932 4387 618
Size: 325 217 627 1145 1455 1842
Fragments arranged by size:
1842 1455 1145 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2543 2930 1391
Size: 622 530 387 4072
Fragments arranged by size:
4072 622 530 387
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2155 2591 928
Size: 971 256 436 3948
Fragments arranged by size:
3948 971 436 256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3802 3836 1903
Size: 34 1865 34 3678
Fragments arranged by size:
3678 1865 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3503 4713 3503
Size: 1210 4401
BclI T'GATC_A
Cuts at: 1548 2298 2511 1548
Size: 750 213 4648
Fragments arranged by size:
4648 750 213
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2022 2742 2891
Size: 107 122 71 631 605 350 720 149
Cuts at: 2891 4222 136
Size: 1331 1525
Fragments arranged by size:
1525 1331 720 631 605 350 149 122
107 71
BglII A'GATC_T
Cuts at: 5606 5606
Size: 5611
BlpI GC'TnA_GC
Cuts at: 3028 3028
Size: 5611
Bme1580I G_kGCm'C
Cuts at: 1790 2219 2543 2856 3672 4828 1790
Size: 429 324 313 816 1156 2573
Fragments arranged by size:
2573 1156 816 429 324 313
BmgBI CAC'GTC
Cuts at: 1318 2509 2851 1318
Size: 1191 342 4078
Fragments arranged by size:
4078 1191 342
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3342 4300 442
Size: 416 53 83 399 24 1925 958 1753
Fragments arranged by size:
1925 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4271 1239
Size: 3032 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2579 3736 4601 4899 2579
Size: 1157 865 298 3291
Fragments arranged by size:
3291 1157 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2066 4274 882
Size: 617 567 2208 2219
Fragments arranged by size:
2219 2208 617 567
BsaAI yAC'GTr
Cuts at: 493 1204 3402 493
Size: 711 2198 2702
Fragments arranged by size:
2702 2198 711
BsaBI GATnn'nnATC
Cuts at: 5605 5605
Size: 5611
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3418 3800 4435 275
Size: 53 83 186 936 1885 382 635 1451
Fragments arranged by size:
1885 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4044 4442 4716 4863 5274 4044
Size: 398 274 147 411 4381
Fragments arranged by size:
4381 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2219 2249 2828 2858 1178
Size: 30 165 30 816 30 579 30 3931
Fragments arranged by size:
3931 816 579 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2515 1186
Size: 1329 4282
BseYI C'CCAG_C
Cuts at: 2729 4814 5310 2729
Size: 2085 496 3030
Fragments arranged by size:
3030 2085 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2624 2695 2776 2813 1163
Size: 573 888 71 81 37 3961
Fragments arranged by size:
3961 888 573 81 71 37
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3061 3822 4850 1540
Size: 275 186 1060 761 1028 2301
Fragments arranged by size:
2301 1060 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2219 2774 2856 3672 3757 4828 2219
Size: 555 82 816 85 1071 3002
Fragments arranged by size:
3002 1071 816 555 85 82
BsmI GAATG_Cn'
Cuts at: 3122 3215 3122
Size: 93 5518
BsmBI CGTCTCn'nnnn_
Cuts at: 2220 2220
Size: 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2219 2543 2774 2856 2930 3672
Size: 399 223 206 324 231 82 74 742
Cuts at: 3672 3757 4828 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 324 231 223 206
85 82 74
BspEI T'CCGG_A
Cuts at: 5274 5274
Size: 5611
BspHI T'CATG_A
Cuts at: 2481 2580 3500 5230 2481
Size: 99 920 1730 2862
Fragments arranged by size:
2862 1730 920 99
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2084 2101 2268 2803 2966 844
Size: 1240 17 167 535 163 3489
Fragments arranged by size:
3489 1240 535 167 163 17
BsrBI CCG'CTC
Cuts at: 1394 1823 3498 1394
Size: 429 1675 3507
Fragments arranged by size:
3507 1675 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4106 4280 65
Size: 1183 2858 174 1396
Fragments arranged by size:
2858 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2133 2788 4255 4442 1700
Size: 110 323 655 1467 187 2869
Fragments arranged by size:
2869 1467 655 323 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5611
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5611
BssSI C'ACGA_G
Cuts at: 3671 4683 3671
Size: 1012 4599
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5611
BstBI TT'CG_AA
Cuts at: 4415 4415
Size: 5611
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5611
BstYI r'GATC_y
Cuts at: 1972 2941 3308 3693 3710 5162 5248 5260 5606
Size: 969 367 385 17 1452 86 12 346
Cuts at: 5606 1972
Size: 1977
Fragments arranged by size:
1977 1452 969 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2352 2361 2535 2691 513
Size: 552 939 348 9 174 156 3433
Fragments arranged by size:
3433 939 552 348 174 156 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4872
Fragments arranged by size:
4872 683 56
BtsI GCAGTG_nn'
Cuts at: 3127 3921 3941 3127
Size: 794 20 4797
Fragments arranged by size:
4797 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5611
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5576
DraI TTT'AAA
Cuts at: 2915 2915
Size: 5611
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2159 2387 4618 809
Size: 507 843 228 2231 1802
Fragments arranged by size:
2231 1802 843 507 228
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2092 2424 2734
Size: 54 1425 50 275 18 262 332 310
Cuts at: 2734 3058 3947 5576 8
Size: 324 889 1629 43
Fragments arranged by size:
1629 1425 889 332 324 310 275 262
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3058 1537
Size: 275 1246 4090
Fragments arranged by size:
4090 1246 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2561 3541 1353
Size: 1208 980 3423
Fragments arranged by size:
3423 1208 980
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2325 4198 4572 4718 387
Size: 1064 138 736 1873 374 146 1280
Fragments arranged by size:
1873 1280 1064 736 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2852 3674 4271 5058 5326 1239
Size: 393 1220 822 597 787 268 1524
Fragments arranged by size:
1524 1220 822 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2330 2654 2330
Size: 324 5287
EcoO109I rG'GnC_Cy
Cuts at: 2098 2540 2893 2098
Size: 442 353 4816
Fragments arranged by size:
4816 442 353
EcoRI G'AATT_C
Cuts at: 2920 3298 2920
Size: 378 5233
FspI TGC'GCA
Cuts at: 4117 4117
Size: 5611
HaeII r_GCGC'y
Cuts at: 1678 2140 4758 1678
Size: 462 2618 2531
Fragments arranged by size:
2618 2531 462
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2219 2251 4252 4284 4326
Size: 32 135 32 647 32 2001 32 42
Cuts at: 4326 4358 1373
Size: 32 2626
Fragments arranged by size:
2626 2001 647 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2955 3201 669
Size: 988 281 1017 246 3079
Fragments arranged by size:
3079 1017 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5611
HpaI GTT'AAC
Cuts at: 3201 3201
Size: 5611
KpnI G_GTAC'C
Cuts at: 2936 2936
Size: 5611
MfeI C'AATT_G
Cuts at: 3210 3210
Size: 5611
MluI A'CGCG_T
Cuts at: 5487 5487
Size: 5611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2178 2871 4725 4909 1305
Size: 873 693 1854 184 2007
Fragments arranged by size:
2007 1854 873 693 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2426 10
Size: 54 1425 343 594 3195
Fragments arranged by size:
3195 1425 594 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2463 3569 3928 4087 518
Size: 648 1297 1106 359 159 2042
Fragments arranged by size:
2042 1297 1106 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2733 2800 2830 3027 3704 4852 5097
Size: 516 611 67 30 197 677 1148 245
Cuts at: 5097 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 611 516 245 197 67
30
NcoI C'CATG_G
Cuts at: 513 1065 2004 2535 513
Size: 552 939 531 3589
Fragments arranged by size:
3589 939 552 531
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5611
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5611
NotI GC'GGCC_GC
Cuts at: 3058 3058
Size: 5611
NspI r_CATG'y
Cuts at: 1132 1229 2462 2969 4514 1132
Size: 97 1233 507 1545 2229
Fragments arranged by size:
2229 1545 1233 507 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5611
PciI A'CATG_T
Cuts at: 2458 4510 2458
Size: 2052 3559
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5611
PfoI T'CCnGG_A
Cuts at: 2781 2781
Size: 5611
PmeI GTTT'AAAC
Cuts at: 2915 2915
Size: 5611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2048 2080 3680 3712 5193 5225 2048
Size: 32 1600 32 1481 32 2434
Fragments arranged by size:
2434 1600 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5611
PsiI TTA'TAA
Cuts at: 3181 3181
Size: 5611
PspOMI G'GGCC_C
Cuts at: 2539 2539
Size: 5611
PstI C_TGCA'G
Cuts at: 830 2081 2112 2273 2963 830
Size: 1251 31 161 690 3478
Fragments arranged by size:
3478 1251 690 161 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5611
PvuII CAG'CTG
Cuts at: 2122 2830 2122
Size: 708 4903
SalI G'TCGA_C
Cuts at: 1936 2953 1936
Size: 1017 4594
SbfI CC_TGCA'GG
Cuts at: 2963 2963
Size: 5611
ScaI AGT'ACT
Cuts at: 2837 3859 5571 2837
Size: 1022 1712 2877
Fragments arranged by size:
2877 1712 1022
SfcI C'TryA_G
Cuts at: 826 1045 2077 2108 2269 2959 4094 4775 4966
Size: 219 1032 31 161 690 1135 681 191
Cuts at: 4966 826
Size: 1471
Fragments arranged by size:
1471 1135 1032 690 681 219 191 161
31
SfiI GGCCn_nnn'nGGCC
Cuts at: 2022 2022
Size: 5611
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
SgrAI Cr'CCGG_yG
Cuts at: 1700 2133 1700
Size: 433 5178
SmaI CCC'GGG
Cuts at: 1872 2938 1872
Size: 1066 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2594 3715 4616 4878 820
Size: 197 926 651 1121 901 262 1553
Fragments arranged by size:
1553 1121 926 901 651 262 197
SnaBI TAC'GTA
Cuts at: 493 3402 493
Size: 2909 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5611
SphI G_CATG'C
Cuts at: 1132 2969 1132
Size: 1837 3774
SspI AAT'ATT
Cuts at: 5 52 3535 5
Size: 47 3483 2081
Fragments arranged by size:
3483 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5611
StyI C'CwwG_G
Cuts at: 513 1065 2004 2535 3050 513
Size: 552 939 531 515 3074
Fragments arranged by size:
3074 939 552 531 515
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2453 3638 3655 3808 3993 4438 325
Size: 1255 873 1185 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1185 873 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2835 3857 5569
Size: 274 80 33 51 1443 858 1022 1712
Cuts at: 5569 96
Size: 138
Fragments arranged by size:
1712 1443 1022 858 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5571 5603 5571
Size: 32 5579
Tth111I GACn'n_nGTC
Cuts at: 1316 2204 1316
Size: 888 4723
XbaI T'CTAG_A
Cuts at: 2947 2947
Size: 5611
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5611
XmaI C'CCGG_G
Cuts at: 1870 2936 1870
Size: 1066 4545
XmnI GAAnn'nnTTC
Cuts at: 3740 3740
Size: 5611
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3419 276
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfoI SrfI SwaI
XcmI