Restriction map for
pFN21AE3538
(Circular) (Six-base) MAPSORT of: pFN21AE3538.seq Check: 7729 from: 1 to: 7162
pFN21AE3538 7162 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2730 2730
Size: 7162
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4972 278
Size: 53 83 186 936 3436 2468
Fragments arranged by size:
3436 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4505 1937
Size: 2568 4594
Acc65I G'GTAC_C
Cuts at: 4483 4483
Size: 7162
AclI AA'CG_TT
Cuts at: 1563 5289 5662 1563
Size: 3726 373 3063
Fragments arranged by size:
3726 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2332 3207 3435 4031 5225 6609 6877 1632
Size: 700 875 228 596 1194 1384 268 1917
Fragments arranged by size:
1917 1384 1194 875 700 596 268 228
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6965
AflIII A'CryG_T
Cuts at: 1203 4086 6061 7038 1203
Size: 2883 1975 977 1327
Fragments arranged by size:
2883 1975 1327 977
AgeI A'CCGG_T
Cuts at: 5993 5993
Size: 7162
AhdI GACnn_n'nnGTC
Cuts at: 5891 5891
Size: 7162
AleI CACnn'nnGTG
Cuts at: 1166 2738 2985 1166
Size: 1572 247 5343
Fragments arranged by size:
5343 1572 247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2328 2568 3405 1184
Size: 1144 240 837 4941
Fragments arranged by size:
4941 1144 837 240
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2673 2705 2673
Size: 32 7130
AlwNI CAG_nnn'CTG
Cuts at: 2122 2927 3104 4297 4905 6477 2122
Size: 805 177 1193 608 1572 2807
Fragments arranged by size:
2807 1572 1193 805 608 177
ApaI G_GGCC'C
Cuts at: 2228 3128 3331 2228
Size: 900 203 6059
Fragments arranged by size:
6059 900 203
ApaLI G'TGCA_C
Cuts at: 3598 5219 6375 3598
Size: 1621 1156 4385
Fragments arranged by size:
4385 1621 1156
ApoI r'AATT_y
Cuts at: 1493 4168 4445 4471 4697 4849 6958 1493
Size: 2675 277 26 226 152 2109 1697
Fragments arranged by size:
2675 2109 1697 277 226 152 26
AseI AT'TA_AT
Cuts at: 160 1032 5716 160
Size: 872 4684 1606
Fragments arranged by size:
4684 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2227 2276 2830 3230 3999 4487
Size: 73 271 13 49 554 400 769 488
Cuts at: 4487 1870
Size: 4545
Fragments arranged by size:
4545 769 554 488 400 271 73 49
13
BamHI G'GATC_C
Cuts at: 4492 4859 4492
Size: 367 6795
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2218 2327 3440 4483 5938
Size: 325 217 627 431 109 1113 1043 1455
Cuts at: 5938 618
Size: 1842
Fragments arranged by size:
1842 1455 1113 1043 627 431 325 217
109
BanII G_rGCy'C
Cuts at: 1391 2228 2895 3128 3171 3204 3331 3403 3795
Size: 837 667 233 43 33 127 72 392
Cuts at: 3795 4221 4481 1391
Size: 426 260 4072
Fragments arranged by size:
4072 837 667 426 392 260 233 127
72 43 33
BbeI G_GCGC'C
Cuts at: 3444 3444
Size: 7162
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5353 5387 1903
Size: 34 3416 34 3678
Fragments arranged by size:
3678 3416 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3025 5054 6264 3025
Size: 2029 1210 3923
Fragments arranged by size:
3923 2029 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7162
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2216 2953 5773
Size: 107 122 71 631 605 544 737 2820
Cuts at: 5773 136
Size: 1525
Fragments arranged by size:
2820 1525 737 631 605 544 122 107
71
BglII A'GATC_T
Cuts at: 3861 7157 3861
Size: 3296 3866
BlpI GC'TnA_GC
Cuts at: 2528 3704 4579 2528
Size: 1176 875 5111
Fragments arranged by size:
5111 1176 875
Bme1580I G_kGCm'C
Cuts at: 1790 2221 2228 2445 2942 3128 3331 3602 5223
Size: 431 7 217 497 186 203 271 1621
Cuts at: 5223 6379 1790
Size: 1156 2573
Fragments arranged by size:
2573 1621 1156 497 431 271 217 203
186 7
BmgBI CAC'GTC
Cuts at: 1318 2590 3673 1318
Size: 1272 1083 4807
Fragments arranged by size:
4807 1272 1083
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4893 5851 442
Size: 416 53 83 399 24 3476 958 1753
Fragments arranged by size:
3476 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7162
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2934 4087 2934
Size: 1153 6009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 2225 2306 2357 2467 2615 2784 2858
Size: 965 21 81 51 110 148 169 74
Cuts at: 2858 2904 2934 3081 5822 1239
Size: 46 30 147 2741 2579
Fragments arranged by size:
2741 2579 965 169 148 147 110 81
74 51 46 30 21
Bpu10I CC'TnA_GC
Cuts at: 2889 3372 3378 2889
Size: 483 6 6673
Fragments arranged by size:
6673 483 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3899 5287 6152 6450 3899
Size: 1388 865 298 4611
Fragments arranged by size:
4611 1388 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2671 2830 3277 3515 4154 5825 882
Size: 617 1172 159 447 238 639 1671 2219
Fragments arranged by size:
2219 1671 1172 639 617 447 238 159
BsaAI yAC'GTr
Cuts at: 493 1204 3712 3778 4953 493
Size: 711 2508 66 1175 2702
Fragments arranged by size:
2702 2508 1175 711 66
BsaBI GATnn'nnATC
Cuts at: 3011 3689 7156 3011
Size: 678 3467 3017
Fragments arranged by size:
3467 3017 678
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3441 3735 4969 5351
Size: 53 83 186 936 1908 294 1234 382
Cuts at: 5351 5986 275
Size: 635 1451
Fragments arranged by size:
1908 1451 1234 936 635 382 294 186
83 53
BsaWI w'CCGG_w
Cuts at: 3403 3801 5595 5993 6267 6414 6825 3403
Size: 398 1794 398 274 147 411 3740
Fragments arranged by size:
3740 1794 411 398 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2673 2703 3079 3109 1178
Size: 30 165 30 1270 30 376 30 5231
Fragments arranged by size:
5231 1270 376 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2464 2492 3263 3266 4241 1186
Size: 1278 28 771 3 975 4107
Fragments arranged by size:
4107 1278 975 771 28 3
BseYI C'CCAG_C
Cuts at: 2267 2319 2638 2698 2765 3108 3263 4188 6365
Size: 52 319 60 67 343 155 925 2177
Cuts at: 6365 6861 2267
Size: 496 2568
Fragments arranged by size:
2568 2177 925 496 343 319 155 67
60 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2108 2180 2874 3411 3742 3869 3887
Size: 573 372 72 694 537 331 127 18
Cuts at: 3887 1163
Size: 4438
Fragments arranged by size:
4438 694 573 537 372 331 127 72
18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3802 4612 5373 6401 1540
Size: 275 186 1801 810 761 1028 2301
Fragments arranged by size:
2301 1801 1028 810 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2534 3204 3602 3795 5223 5308 6379 2534
Size: 670 398 193 1428 85 1071 3317
Fragments arranged by size:
3317 1428 1071 670 398 193 85
BsmI GAATG_Cn'
Cuts at: 2708 4673 4766 2708
Size: 1965 93 5104
Fragments arranged by size:
5104 1965 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4358 4358
Size: 7162
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2221 2228 2445 2534 2895 2942 3128
Size: 399 431 7 217 89 361 47 186
Cuts at: 3128 3171 3204 3331 3403 3602 3795 4221 4481
Size: 43 33 127 72 199 193 426 260
Cuts at: 4481 5223 5308 6379 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 431 426 399 361 260
217 199 193 186 127 89 85 72
47 43 33 7
BspEI T'CCGG_A
Cuts at: 6825 6825
Size: 7162
BspHI T'CATG_A
Cuts at: 3693 5051 6781 3693
Size: 1358 1730 4074
Fragments arranged by size:
4074 1730 1358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2072 2730 3891 4517 844
Size: 1228 658 1161 626 3489
Fragments arranged by size:
3489 1228 1161 658 626
BsrBI CCG'CTC
Cuts at: 1394 1823 2188 5049 1394
Size: 429 365 2861 3507
Fragments arranged by size:
3507 2861 429 365
BsrDI GCAATG_nn'
Cuts at: 65 1248 5657 5831 65
Size: 1183 4409 174 1396
Fragments arranged by size:
4409 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5806 5993 1700
Size: 110 3996 187 2869
Fragments arranged by size:
3996 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7162
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7162
BssSI C'ACGA_G
Cuts at: 2908 3280 3317 4304 5222 6234 2908
Size: 372 37 987 918 1012 3836
Fragments arranged by size:
3836 1012 987 918 372 37
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2064 2852 3883 794
Size: 1270 788 1031 4073
Fragments arranged by size:
4073 1270 1031 788
BstBI TT'CG_AA
Cuts at: 5966 5966
Size: 7162
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7162
BstXI CCAn_nnnn'nTGG
Cuts at: 2383 2684 2740 2987 3158 3323 2383
Size: 301 56 247 171 165 6222
Fragments arranged by size:
6222 301 247 171 165 56
BstYI r'GATC_y
Cuts at: 1972 3006 3861 4340 4492 4859 5244 5261 6713
Size: 1034 855 479 152 367 385 17 1452
Cuts at: 6713 6799 6811 7157 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1034 855 479 385 367 346
152 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2475 2777 2475
Size: 302 6860
BtgI C'CryG_G
Cuts at: 513 1065 2076 513
Size: 552 1011 5599
Fragments arranged by size:
5599 1011 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2362 487
Size: 683 56 1136 5287
Fragments arranged by size:
5287 1136 683 56
BtsI GCAGTG_nn'
Cuts at: 2667 4678 5472 5492 2667
Size: 2011 794 20 4337
Fragments arranged by size:
4337 2011 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7127
DraI TTT'AAA
Cuts at: 4466 4466
Size: 7162
DraIII CAC_nnn'GTG
Cuts at: 2852 4293 2852
Size: 1441 5721
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6169 809
Size: 507 4853 1802
Fragments arranged by size:
4853 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2716 3294 3583
Size: 54 1425 50 275 18 886 578 289
Cuts at: 3583 3981 4609 5498 7127 8
Size: 398 628 889 1629 43
Fragments arranged by size:
1629 1425 889 886 628 578 398 289
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4609 1537
Size: 275 2797 4090
Fragments arranged by size:
4090 2797 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2351 2496 2565 3644 3800 4262 5092 1353
Size: 998 145 69 1079 156 462 830 3423
Fragments arranged by size:
3423 1079 998 830 462 156 145 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2432 2600 5749 6123 6269 387
Size: 1064 138 843 168 3149 374 146 1280
Fragments arranged by size:
3149 1280 1064 843 374 168 146 138
EcoICRI GAG'CTC
Cuts at: 3202 3793 3202
Size: 591 6571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2225 2306 2332 2357 2467 2615
Size: 393 572 21 81 26 25 110 148
Cuts at: 2615 2784 2858 2904 2934 3081 3207 3435 4031
Size: 169 74 46 30 147 126 228 596
Cuts at: 4031 5225 5822 6609 6877 1239
Size: 1194 597 787 268 1524
Fragments arranged by size:
1524 1194 787 597 596 572 393 268
228 169 148 147 126 110 81 74
46 30 26 25 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2429 3341 2429
Size: 912 6250
EcoO109I rG'GnC_Cy
Cuts at: 2191 2281 2341 2924 2945 3124 3327 3328 2191
Size: 90 60 583 21 179 203 1 6025
Fragments arranged by size:
6025 583 203 179 90 60 21 1
EcoRI G'AATT_C
Cuts at: 4471 4849 4471
Size: 378 6784
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 4036 2083
Size: 1953 5209
FspI TGC'GCA
Cuts at: 5668 5668
Size: 7162
HaeII r_GCGC'y
Cuts at: 1678 3145 3444 3657 6309 1678
Size: 1467 299 213 2652 2531
Fragments arranged by size:
2652 2531 1467 299 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3019 3051 3066 3098 3937
Size: 32 135 32 1447 32 15 32 839
Cuts at: 3937 3969 5803 5835 5877 5909 1373
Size: 32 1834 32 42 32 2626
Fragments arranged by size:
2626 1834 1447 839 135 42 32 32
32 32 32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 4506 4752 669
Size: 988 281 2568 246 3079
Fragments arranged by size:
3079 2568 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3289 4332 748
Size: 2541 1043 3578
Fragments arranged by size:
3578 2541 1043
HpaI GTT'AAC
Cuts at: 4752 4752
Size: 7162
KasI G'GCGC_C
Cuts at: 3440 3440
Size: 7162
KpnI G_GTAC'C
Cuts at: 4487 4487
Size: 7162
MfeI C'AATT_G
Cuts at: 4761 4761
Size: 7162
MluI A'CGCG_T
Cuts at: 7038 7038
Size: 7162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4475 6276 6460 1305
Size: 3170 1801 184 2007
Fragments arranged by size:
3170 2007 1801 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2718 3296 3585 3983 10
Size: 54 1425 343 886 578 289 398 3189
Fragments arranged by size:
3189 1425 886 578 398 343 289 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2738 2985 3644 4085 4112 4413 5120
Size: 648 1572 247 659 441 27 301 707
Cuts at: 5120 5479 5638 518
Size: 359 159 2042
Fragments arranged by size:
2042 1572 707 659 648 441 359 301
247 159 27
MspA1I CmG'CkG
Cuts at: 1606 2606 3104 3211 3756 4578 5255 6403 6648
Size: 1000 498 107 545 822 677 1148 245
Cuts at: 6648 1606
Size: 2120
Fragments arranged by size:
2120 1148 1000 822 677 545 498 245
107
NarI GG'CG_CC
Cuts at: 3441 3441
Size: 7162
NcoI C'CATG_G
Cuts at: 513 1065 2076 513
Size: 552 1011 5599
Fragments arranged by size:
5599 1011 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7162
NotI GC'GGCC_GC
Cuts at: 4609 4609
Size: 7162
NspI r_CATG'y
Cuts at: 1132 1229 2098 2164 2525 4090 4117 4520 6065
Size: 97 869 66 361 1565 27 403 1545
Cuts at: 6065 1132
Size: 2229
Fragments arranged by size:
2229 1565 1545 869 403 361 97 66
27
PasI CC'CwG_GG
Cuts at: 1348 2942 3158 3331 3356 1348
Size: 1594 216 173 25 5154
Fragments arranged by size:
5154 1594 216 173 25
PciI A'CATG_T
Cuts at: 4086 6061 4086
Size: 1975 5187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2662 2927 1108
Size: 1554 265 5343
Fragments arranged by size:
5343 1554 265
PfoI T'CCnGG_A
Cuts at: 2283 2283
Size: 7162
PmeI GTTT'AAAC
Cuts at: 4466 4466
Size: 7162
PmlI CAC'GTG
Cuts at: 3712 3712
Size: 7162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2673 2705 5231 5263 6744 6776 2673
Size: 32 2526 32 1481 32 3059
Fragments arranged by size:
3059 2526 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2281 2281
Size: 7162
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7162
PsiI TTA'TAA
Cuts at: 4732 4732
Size: 7162
PspOMI G'GGCC_C
Cuts at: 2224 3124 3327 2224
Size: 900 203 6059
Fragments arranged by size:
6059 900 203
PstI C_TGCA'G
Cuts at: 830 3216 3342 4277 4377 4514 830
Size: 2386 126 935 100 137 3478
Fragments arranged by size:
3478 2386 935 137 126 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7162
PvuII CAG'CTG
Cuts at: 3104 3756 3104
Size: 652 6510
SacI G_AGCT'C
Cuts at: 3204 3795 3204
Size: 591 6571
SalI G'TCGA_C
Cuts at: 1936 4504 1936
Size: 2568 4594
SapI GCTCTTCn'nnn_
Cuts at: 3644 3800 4262 3644
Size: 156 462 6544
Fragments arranged by size:
6544 462 156
SbfI CC_TGCA'GG
Cuts at: 4514 4514
Size: 7162
ScaI AGT'ACT
Cuts at: 5410 7122 5410
Size: 1712 5450
SexAI A'CCwGG_T
Cuts at: 2848 2848
Size: 7162
SfcI C'TryA_G
Cuts at: 826 1045 3212 3338 4183 4273 4373 4510 5645
Size: 219 2167 126 845 90 100 137 1135
Cuts at: 5645 6326 6517 826
Size: 681 191 1471
Fragments arranged by size:
2167 1471 1135 845 681 219 191 137
126 100 90
SfoI GGC'GCC
Cuts at: 3442 3442
Size: 7162
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7162
SmaI CCC'GGG
Cuts at: 1872 2216 2229 3232 4489 1872
Size: 344 13 1003 1257 4545
Fragments arranged by size:
4545 1257 1003 344 13
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3914 3999 5266 6167 6429 820
Size: 197 926 1971 85 1267 901 262 1553
Fragments arranged by size:
1971 1553 1267 926 901 262 197 85
SnaBI TAC'GTA
Cuts at: 493 4953 493
Size: 4460 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7162
SphI G_CATG'C
Cuts at: 1132 4520 1132
Size: 3388 3774
SrfI GCCC'GGGC
Cuts at: 2229 2229
Size: 7162
SspI AAT'ATT
Cuts at: 5 52 5086 5
Size: 47 5034 2081
Fragments arranged by size:
5034 2081 47
StuI AGG'CCT
Cuts at: 1517 2210 2881 3618 1517
Size: 693 671 737 5061
Fragments arranged by size:
5061 737 693 671
StyI C'CwwG_G
Cuts at: 513 1065 2019 2076 2748 3002 3070 3657 4233
Size: 552 954 57 672 254 68 587 576
Cuts at: 4233 4601 513
Size: 368 3074
Fragments arranged by size:
3074 954 672 587 576 552 368 254
68 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2259 2276 2779 5189 5206 5359 5544
Size: 1255 679 17 503 2410 17 153 185
Cuts at: 5544 5989 325
Size: 445 1498
Fragments arranged by size:
2410 1498 1255 679 503 445 185 153
17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5408 7120 96
Size: 274 80 33 51 1443 3431 1712 138
Fragments arranged by size:
3431 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3079 3111 7122 7154 3079
Size: 32 4011 32 3087
Fragments arranged by size:
4011 3087 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7162
XbaI T'CTAG_A
Cuts at: 4498 4498
Size: 7162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2026 2383 2711 2026
Size: 357 328 6477
Fragments arranged by size:
6477 357 328
XhoI C'TCGA_G
Cuts at: 1943 3999 1943
Size: 2056 5106
XmaI C'CCGG_G
Cuts at: 1870 2214 2227 3230 4487 1870
Size: 344 13 1003 1257 4545
Fragments arranged by size:
4545 1257 1003 344 13
XmnI GAAnn'nnTTC
Cuts at: 4409 5291 4409
Size: 882 6280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4970 276
Size: 53 83 186 936 3436 2468
Fragments arranged by size:
3436 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspXI PsrI RsrII SacII SanDI SfiI SwaI