Restriction map for
pFN21AE3532
(Circular) (Six-base) MAPSORT of: pFN21AE3532.seq Check: 8068 from: 1 to: 5956
pFN21AE3532 5956 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2071 2071
Size: 5956
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4019 5403 5671 1632
Size: 2387 1384 268 1917
Fragments arranged by size:
2387 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 3084 1166
Size: 1918 4038
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2964 1184
Size: 1780 4176
AlwNI CAG_nnn'CTG
Cuts at: 2914 3699 5271 2914
Size: 785 1572 3599
Fragments arranged by size:
3599 1572 785
ApaI G_GGCC'C
Cuts at: 2280 2515 2280
Size: 235 5721
ApaLI G'TGCA_C
Cuts at: 2894 4013 5169 2894
Size: 1119 1156 3681
Fragments arranged by size:
3681 1156 1119
ApoI r'AATT_y
Cuts at: 1493 3265 3491 3643 5752 1493
Size: 1772 226 152 2109 1697
Fragments arranged by size:
2109 1772 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 1870
Size: 73 1338 4545
Fragments arranged by size:
4545 1338 73
AvrII C'CTAG_G
Cuts at: 3137 3137
Size: 5956
BamHI G'GATC_C
Cuts at: 3178 3286 3653 3178
Size: 108 367 5481
Fragments arranged by size:
5481 367 108
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2047 2493 2605 2672
Size: 325 217 627 242 18 446 112 67
Cuts at: 2672 3036 3277 4732 618
Size: 364 241 1455 1842
Fragments arranged by size:
1842 1455 627 446 364 325 242 241
217 112 67 18
BanII G_rGCy'C
Cuts at: 1391 2280 2515 3275 1391
Size: 889 235 760 4072
Fragments arranged by size:
4072 889 760 235
BbeI G_GCGC'C
Cuts at: 2497 2609 2676 2497
Size: 112 67 5777
Fragments arranged by size:
5777 112 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4985
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2290 2603 2699
Size: 107 122 71 631 605 618 313 96
Cuts at: 2699 4567 136
Size: 1868 1525
Fragments arranged by size:
1868 1525 631 618 605 313 122 107
96 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2163 2280 2503 2515 2898 2985 4017 5173
Size: 373 117 223 12 383 87 1032 1156
Cuts at: 5173 1790
Size: 2573
Fragments arranged by size:
2573 1156 1032 383 373 223 117 87
12
BmgBI CAC'GTC
Cuts at: 1318 3086 1318
Size: 1768 4188
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3117 3687 4645
Size: 416 53 83 399 24 1700 570 958
Cuts at: 4645 442
Size: 1753
Fragments arranged by size:
1753 1700 958 570 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2429 3019 2429
Size: 590 5366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2648 4616 1239
Size: 1409 1968 2579
Fragments arranged by size:
2579 1968 1409
Bpu10I CC'TnA_GC
Cuts at: 2915 2915
Size: 5956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4619 882
Size: 617 3120 2219
Fragments arranged by size:
3120 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2494 2606 2673 3763
Size: 53 83 186 936 961 112 67 1090
Cuts at: 3763 4145 4780 275
Size: 382 635 1451
Fragments arranged by size:
1451 1090 961 936 635 382 186 112
83 67 53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5731
Fragments arranged by size:
5731 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3194 1186
Size: 2008 3948
BseYI C'CCAG_C
Cuts at: 2133 2978 5159 5655 2133
Size: 845 2181 496 2434
Fragments arranged by size:
2434 2181 845 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5383
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2393 2661 3406 4167 5195 1540
Size: 275 186 392 268 745 761 1028 2301
Fragments arranged by size:
2301 1028 761 745 392 275 268 186
BsiHKAI G_wGCw'C
Cuts at: 2484 2898 2921 3015 3032 4017 4102 5173 2484
Size: 414 23 94 17 985 85 1071 3267
Fragments arranged by size:
3267 1071 985 414 94 85 23 17
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
BsmBI CGTCTCn'nnnn_
Cuts at: 2174 2174
Size: 5956
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2280 2484 2503 2515 2898 2921
Size: 399 373 117 204 19 12 383 23
Cuts at: 2921 2985 3015 3032 3275 4017 4102 5173 1391
Size: 64 30 17 243 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 383 373 243 204
117 85 64 30 23 19 17 12
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2071 2932 3311 844
Size: 1227 861 379 3489
Fragments arranged by size:
3489 1227 861 379
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2742 2898 3211 4600 4787 1700
Size: 110 932 156 313 1389 187 2869
Fragments arranged by size:
2869 1389 932 313 187 156 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 2562 1925
Size: 637 5319
BssSI C'ACGA_G
Cuts at: 4016 5028 4016
Size: 1012 4944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3036 794
Size: 2242 3714
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 2272 2875 3182 2272
Size: 603 307 5046
Fragments arranged by size:
5046 603 307
BstYI r'GATC_y
Cuts at: 1972 3178 3286 3653 4038 4055 5507 5593 5605
Size: 1206 108 367 385 17 1452 86 12
Cuts at: 5605 5951 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1206 385 367 346 108 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2676 513
Size: 552 939 672 3793
Fragments arranged by size:
3793 939 672 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2678 487
Size: 683 56 1452 3765
Fragments arranged by size:
3765 1452 683 56
BtsI GCAGTG_nn'
Cuts at: 3472 4266 4286 3472
Size: 794 20 5142
Fragments arranged by size:
5142 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DraIII CAC_nnn'GTG
Cuts at: 3036 3036
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2390 2658 3051
Size: 54 1425 50 275 18 560 268 393
Cuts at: 3051 3209 3403 4292 5921 8
Size: 158 194 889 1629 43
Fragments arranged by size:
1629 1425 889 560 393 275 268 194
158 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2390 2658 3403 1537
Size: 275 578 268 745 4090
Fragments arranged by size:
4090 745 578 275 268
EarI CTCTTCn'nnn_
Cuts at: 1353 2120 2237 2849 3886 1353
Size: 767 117 612 1037 3423
Fragments arranged by size:
3423 1037 767 612 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2804 4543 4917 5063 387
Size: 1064 138 1215 1739 374 146 1280
Fragments arranged by size:
1739 1280 1215 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2648 4019 4616 5403 5671 1239
Size: 393 1016 1371 597 787 268 1524
Fragments arranged by size:
1524 1371 1016 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2276 2277 2427 2439 2512 2911 3119 3141 2276
Size: 1 150 12 73 399 208 22 5091
Fragments arranged by size:
5091 399 208 150 73 22 12 1
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 2497 2609 2676 5103 1678
Size: 819 112 67 2427 2531
Fragments arranged by size:
2531 2427 819 112 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4597 4629 4671 4703 1373
Size: 32 135 32 3025 32 42 32 2626
Fragments arranged by size:
3025 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KasI G'GCGC_C
Cuts at: 2493 2605 2672 2493
Size: 112 67 5777
Fragments arranged by size:
5777 112 67
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4134
Fragments arranged by size:
4134 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3084 3914 4273 4432 518
Size: 648 1918 830 359 159 2042
Fragments arranged by size:
2042 1918 830 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2087 3372 4049 5197 5442 1606
Size: 481 1285 677 1148 245 2120
Fragments arranged by size:
2120 1285 1148 677 481 245
NaeI GCC'GGC
Cuts at: 2744 3213 2744
Size: 469 5487
NarI GG'CG_CC
Cuts at: 2494 2606 2673 2494
Size: 112 67 5777
Fragments arranged by size:
5777 112 67
NcoI C'CATG_G
Cuts at: 513 1065 2004 2676 513
Size: 552 939 672 3793
Fragments arranged by size:
3793 939 672 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NgoMIV G'CCGG_C
Cuts at: 2742 3211 2742
Size: 469 5487
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 2658 3403 2658
Size: 745 5211
NspI r_CATG'y
Cuts at: 1132 1229 3314 4859 1132
Size: 97 2085 1545 2229
Fragments arranged by size:
2229 2085 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2074 2508 1348
Size: 726 434 4796
Fragments arranged by size:
4796 726 434
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5956
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2911 2911
Size: 5956
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PspOMI G'GGCC_C
Cuts at: 2276 2511 2276
Size: 235 5721
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2804 2836 2804
Size: 32 5924
PstI C_TGCA'G
Cuts at: 830 2322 3308 830
Size: 1492 986 3478
Fragments arranged by size:
3478 1492 986
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 2087 2087
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SapI GCTCTTCn'nnn_
Cuts at: 2120 2120
Size: 5956
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SexAI A'CCwGG_T
Cuts at: 2793 2823 3032 2793
Size: 30 209 5717
Fragments arranged by size:
5717 209 30
SfcI C'TryA_G
Cuts at: 826 1045 2318 3304 4439 5120 5311 826
Size: 219 1273 986 1135 681 191 1471
Fragments arranged by size:
1471 1273 1135 986 681 219 191
SfoI GGC'GCC
Cuts at: 2495 2607 2674 2495
Size: 112 67 5777
Fragments arranged by size:
5777 112 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4060 4961 5223 820
Size: 197 926 2117 901 262 1553
Fragments arranged by size:
2117 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 2004 2376 2634 2676 2727 3137 3395
Size: 552 939 372 258 42 51 410 258
Cuts at: 3395 513
Size: 3074
Fragments arranged by size:
3074 939 552 410 372 258 258 51
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2657 2946 3983 4000 4153 4338 4783
Size: 1255 1077 289 1037 17 153 185 445
Cuts at: 4783 325
Size: 1498
Fragments arranged by size:
1498 1255 1077 1037 445 289 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4202 5914 96
Size: 274 80 33 51 1443 2225 1712 138
Fragments arranged by size:
2225 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5916 5948 5916
Size: 32 5924
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2872 2875 2933 2872
Size: 3 58 5895
Fragments arranged by size:
5895 58 3
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PspXI RsrII SacI SacII
SanDI SfiI SrfI SwaI