Restriction map for
pFN21AE3530
(Circular) (Six-base) MAPSORT of: pFN21AE3530.seq Check: 5803 from: 1 to: 8425
pFN21AE3530 8425 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2818 3202 3703 4704 5698 2818
Size: 384 501 1001 994 5545
Fragments arranged by size:
5545 1001 994 501 384
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2406 2718 5544 6235
Size: 53 83 186 936 870 312 2826 691
Cuts at: 6235 278
Size: 2468
Fragments arranged by size:
2826 2468 936 870 691 312 186 83
53
AccI GT'mk_AC
Cuts at: 1937 5768 1937
Size: 3831 4594
Acc65I G'GTAC_C
Cuts at: 5746 5746
Size: 8425
AclI AA'CG_TT
Cuts at: 1563 6552 6925 1563
Size: 4989 373 3063
Fragments arranged by size:
4989 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2912 3278 3326 6488 7872 8140 1632
Size: 1280 366 48 3162 1384 268 1917
Fragments arranged by size:
3162 1917 1384 1280 366 268 48
AfeI AGC'GCT
Cuts at: 5644 5644
Size: 8425
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8228
AflIII A'CryG_T
Cuts at: 1203 7324 8301 1203
Size: 6121 977 1327
Fragments arranged by size:
6121 1327 977
AgeI A'CCGG_T
Cuts at: 2358 7256 2358
Size: 4898 3527
AhdI GACnn_n'nnGTC
Cuts at: 3337 7154 3337
Size: 3817 4608
AleI CACnn'nnGTG
Cuts at: 1166 2261 5085 1166
Size: 1095 2824 4506
Fragments arranged by size:
4506 2824 1095
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2279 3674 5420 1184
Size: 1095 1395 1746 4189
Fragments arranged by size:
4189 1746 1395 1095
AlwNI CAG_nnn'CTG
Cuts at: 2243 3433 3853 3862 3934 3979 4466 4538 4633
Size: 1190 420 9 72 45 487 72 95
Cuts at: 4633 5116 6168 7740 2243
Size: 483 1052 1572 2928
Fragments arranged by size:
2928 1572 1190 1052 487 483 420 95
72 72 45 9
ApaI G_GGCC'C
Cuts at: 2317 2485 2801 3672 4291 4800 4857 5310 5444
Size: 168 316 871 619 509 57 453 134
Cuts at: 5444 2317
Size: 5298
Fragments arranged by size:
5298 871 619 509 453 316 168 134
57
ApaLI G'TGCA_C
Cuts at: 6482 7638 6482
Size: 1156 7269
ApoI r'AATT_y
Cuts at: 1493 5734 5960 6112 8221 1493
Size: 4241 226 152 2109 1697
Fragments arranged by size:
4241 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6979 160
Size: 872 5947 1606
Fragments arranged by size:
5947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8425
AvaI C'yCGr_G
Cuts at: 1870 1943 2435 2545 3191 4263 4574 4799 4857
Size: 73 492 110 646 1072 311 225 58
Cuts at: 4857 5310 5437 5750 1870
Size: 453 127 313 4545
Fragments arranged by size:
4545 1072 646 492 453 313 311 225
127 110 73 58
AvrII C'CTAG_G
Cuts at: 2503 2503
Size: 8425
BamHI G'GATC_C
Cuts at: 5755 6122 5755
Size: 367 8058
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2263 3658 3740 3779 3920
Size: 325 217 627 476 1395 82 39 141
Cuts at: 3920 3960 4224 4622 4985 5467 5710 5746 7201
Size: 40 264 398 363 482 243 36 1455
Cuts at: 7201 618
Size: 1842
Fragments arranged by size:
1842 1455 1395 627 482 476 398 363
325 264 243 217 141 82 40 39
36
BanII G_rGCy'C
Cuts at: 1391 2317 2420 2485 2516 2583 2751 2801 2822
Size: 926 103 65 31 67 168 50 21
Cuts at: 2822 2855 2940 3672 4291 4529 4581 4652 4775
Size: 33 85 732 619 238 52 71 123
Cuts at: 4775 4800 4857 5310 5352 5444 5744 1391
Size: 25 57 453 42 92 300 4072
Fragments arranged by size:
4072 926 732 619 453 300 238 168
123 103 92 85 71 67 65 57
52 50 42 33 31 25 21
BbeI G_GCGC'C
Cuts at: 3783 5471 3783
Size: 1688 6737
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2672 3565 928
Size: 971 773 893 5788
Fragments arranged by size:
5788 971 893 773
BbvCI CC'TCA_GC
Cuts at: 2823 4893 5301 5316 2823
Size: 2070 408 15 5932
Fragments arranged by size:
5932 2070 408 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6616 6650 1903
Size: 34 4679 34 3678
Fragments arranged by size:
4679 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2892 6317 7527 2892
Size: 3425 1210 3790
Fragments arranged by size:
3790 3425 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3667 3788 4180
Size: 107 122 71 631 605 1995 121 392
Cuts at: 4180 4841 5243 5335 5370 7036 136
Size: 661 402 92 35 1666 1525
Fragments arranged by size:
1995 1666 1525 661 631 605 402 392
122 121 107 92 71 35
BglII A'GATC_T
Cuts at: 8420 8420
Size: 8425
BlpI GC'TnA_GC
Cuts at: 2948 5842 2948
Size: 2894 5531
Bme1580I G_kGCm'C
Cuts at: 1790 2317 2485 2801 3663 3672 4229 4270 4291
Size: 527 168 316 862 9 557 41 21
Cuts at: 4291 4800 4857 5132 5223 5310 5444 5715 6486
Size: 509 57 275 91 87 134 271 771
Cuts at: 6486 7642 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 862 771 557 527 509 316
275 271 168 134 91 87 57 41
21 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8425
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4621 6156 7114
Size: 416 53 83 399 24 3204 1535 958
Cuts at: 7114 442
Size: 1753
Fragments arranged by size:
3204 1753 1535 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8425
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4024 5527 5611 4024
Size: 1503 84 6838
Fragments arranged by size:
6838 1503 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2308 2500 2530 2681 2774 2888 3386 3662
Size: 1069 192 30 151 93 114 498 276
Cuts at: 3662 4517 4565 4787 4847 5039 7085 1239
Size: 855 48 222 60 192 2046 2579
Fragments arranged by size:
2579 2046 1069 855 498 276 222 192
192 151 114 93 60 48 30
Bpu10I CC'TnA_GC
Cuts at: 2816 2823 4109 4893 5301 5316 2816
Size: 7 1286 784 408 15 5925
Fragments arranged by size:
5925 1286 784 408 15 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3185 3936 6550 7415 7713 3185
Size: 751 2614 865 298 3897
Fragments arranged by size:
3897 2614 865 751 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3515 5006 5212 7088 882
Size: 617 2016 1491 206 1876 2219
Fragments arranged by size:
2219 2016 1876 1491 617 206
BsaAI yAC'GTr
Cuts at: 493 1204 2881 3127 5368 6216 493
Size: 711 1677 246 2241 848 2702
Fragments arranged by size:
2702 2241 1677 848 711 246
BsaBI GATnn'nnATC
Cuts at: 2427 5039 8419 2427
Size: 2612 3380 2433
Fragments arranged by size:
3380 2612 2433
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2157 2403 2715 3780
Size: 53 83 186 936 624 246 312 1065
Cuts at: 3780 4962 5468 5541 5574 6232 6614 7249 275
Size: 1182 506 73 33 658 382 635 1451
Fragments arranged by size:
1451 1182 1065 936 658 635 624 506
382 312 246 186 83 73 53 33
BsaWI w'CCGG_w
Cuts at: 2358 2835 3328 6858 7256 7530 7677 8088 2358
Size: 477 493 3530 398 274 147 411 2695
Fragments arranged by size:
3530 2695 493 477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2195 2225 2503 2533 2990
Size: 30 165 30 792 30 278 30 457
Cuts at: 2990 3020 3512 3542 4639 4669 1178
Size: 30 492 30 1097 30 4934
Fragments arranged by size:
4934 1097 792 492 457 278 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2013 2065 2567 2747 2750 4178 4249 4514
Size: 827 52 502 180 3 1428 71 265
Cuts at: 4514 4517 4589 4969 5309 5333 5516 5687 1186
Size: 3 72 380 340 24 183 171 3924
Fragments arranged by size:
3924 1428 827 502 380 340 265 183
180 171 72 71 52 24 3 3
BseYI C'CCAG_C
Cuts at: 2162 2236 2477 2619 2782 2794 4321 7628 8124
Size: 74 241 142 163 12 1527 3307 496
Cuts at: 8124 2162
Size: 2463
Fragments arranged by size:
3307 2463 1527 496 241 163 142 74
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2687 2882 2981 3238 3731 3899 1163
Size: 573 951 195 99 257 493 168 5689
Fragments arranged by size:
5689 951 573 493 257 195 168 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4066 5875 6636 7664 1540
Size: 275 186 2065 1809 761 1028 2301
Fragments arranged by size:
2301 2065 1809 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2516 3765 4473 4671 5496 6486 6571 7642 2516
Size: 1249 708 198 825 990 85 1071 3299
Fragments arranged by size:
3299 1249 1071 990 825 708 198 85
BsmI GAATG_Cn'
Cuts at: 5936 6029 5936
Size: 93 8332
BsmBI CGTCTCn'nnnn_
Cuts at: 3362 3503 4031 4512 5675 3362
Size: 141 528 481 1163 6112
Fragments arranged by size:
6112 1163 528 481 141
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2317 2420 2485 2516 2583 2751 2801
Size: 399 527 103 65 31 67 168 50
Cuts at: 2801 2822 2855 2940 3663 3672 3765 4229 4270
Size: 21 33 85 723 9 93 464 41
Cuts at: 4270 4291 4473 4529 4581 4652 4671 4775 4800
Size: 21 182 56 52 71 19 104 25
Cuts at: 4800 4857 5132 5223 5310 5352 5444 5496 5715
Size: 57 275 91 87 42 92 52 219
Cuts at: 5715 5744 6486 6571 7642 1391
Size: 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 723 527 464 399 275
219 182 168 104 103 93 92 91
87 85 85 71 67 65 57 56
52 52 50 42 41 33 31 29
25 21 21 19 9
BspEI T'CCGG_A
Cuts at: 2835 3328 8088 2835
Size: 493 4760 3172
Fragments arranged by size:
4760 3172 493
BspHI T'CATG_A
Cuts at: 2625 6314 8044 2625
Size: 3689 1730 3006
Fragments arranged by size:
3689 3006 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2460 2818 3202 3703 3870 4704 5698 5780
Size: 1616 358 384 501 167 834 994 82
Cuts at: 5780 844
Size: 3489
Fragments arranged by size:
3489 1616 994 834 501 384 358 167
82
BsrBI CCG'CTC
Cuts at: 1394 1823 2643 6312 1394
Size: 429 820 3669 3507
Fragments arranged by size:
3669 3507 820 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2287 6920 7094 65
Size: 1183 1039 4633 174 1396
Fragments arranged by size:
4633 1396 1183 1039 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2358 4422 5464 5556 7069 7256 1700
Size: 110 548 2064 1042 92 1513 187 2869
Fragments arranged by size:
2869 2064 1513 1042 548 187 110 92
BsrGI T'GTAC_A
Cuts at: 96 3114 96
Size: 3018 5407
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8425
BssSI C'ACGA_G
Cuts at: 5345 6485 7497 5345
Size: 1140 1012 6273
Fragments arranged by size:
6273 1140 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3862 4629 4666 794
Size: 3068 767 37 4553
Fragments arranged by size:
4553 3068 767 37
BstBI TT'CG_AA
Cuts at: 7229 7229
Size: 8425
BstEII G'GTnAC_C
Cuts at: 1184 3533 1184
Size: 2349 6076
BstXI CCAn_nnnn'nTGG
Cuts at: 3100 3679 3737 5263 3100
Size: 579 58 1526 6262
Fragments arranged by size:
6262 1526 579 58
BstYI r'GATC_y
Cuts at: 1972 2991 3423 4002 5755 6122 6507 6524 7976
Size: 1019 432 579 1753 367 385 17 1452
Cuts at: 7976 8062 8074 8420 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1753 1452 1019 579 432 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3481 3799 3481
Size: 318 8107
BtgI C'CryG_G
Cuts at: 513 1065 2004 3099 3538 3879 4551 513
Size: 552 939 1095 439 341 672 4387
Fragments arranged by size:
4387 1095 939 672 552 439 341
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5026 487
Size: 683 56 3800 3886
Fragments arranged by size:
3886 3800 683 56
BtsI GCAGTG_nn'
Cuts at: 2998 4615 4932 5662 5941 6735 6755 2998
Size: 1617 317 730 279 794 20 4668
Fragments arranged by size:
4668 1617 794 730 317 279 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8390
DraI TTT'AAA
Cuts at: 5729 5729
Size: 8425
DraIII CAC_nnn'GTG
Cuts at: 2596 4629 4945 2596
Size: 2033 316 6076
Fragments arranged by size:
6076 2033 316
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3593 5141 5540 7432 809
Size: 507 2277 1548 399 1892 1802
Fragments arranged by size:
2277 1892 1802 1548 507 399
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2676 3876 4011
Size: 54 1425 50 275 18 846 1200 135
Cuts at: 4011 4063 4143 4548 5526 5601 5872 6761 8390
Size: 52 80 405 978 75 271 889 1629
Cuts at: 8390 8
Size: 43
Fragments arranged by size:
1629 1425 1200 978 889 846 405 275
271 135 80 75 54 52 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4063 5872 1537
Size: 275 2251 1809 4090
Fragments arranged by size:
4090 2251 1809 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2333 2376 3080 3848 3993 4199 5006
Size: 774 206 43 704 768 145 206 807
Cuts at: 5006 6355 1353
Size: 1349 3423
Fragments arranged by size:
3423 1349 807 774 768 704 206 206
145 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2510 4392 4523 4985 7012 7386
Size: 1064 138 921 1882 131 462 2027 374
Cuts at: 7386 7532 387
Size: 146 1280
Fragments arranged by size:
2027 1882 1280 1064 921 462 374 146
138 131
EcoICRI GAG'CTC
Cuts at: 2514 2514
Size: 8425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2308 2500 2530 2681 2774 2888 2912
Size: 393 676 192 30 151 93 114 24
Cuts at: 2912 3278 3326 3386 3662 4517 4565 4787 4847
Size: 366 48 60 276 855 48 222 60
Cuts at: 4847 5039 6488 7085 7872 8140 1239
Size: 192 1449 597 787 268 1524
Fragments arranged by size:
1524 1449 855 787 676 597 393 366
276 268 222 192 192 151 114 93
60 60 48 48 30 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2037 2381 3293 3845 3866 4352 2037
Size: 344 912 552 21 486 6110
Fragments arranged by size:
6110 912 552 486 344 21
EcoO109I rG'GnC_Cy
Cuts at: 2069 2313 2481 2482 2500 2798 2812 3070 3668
Size: 244 168 1 18 298 14 258 598
Cuts at: 3668 3859 3871 4287 4288 4719 4762 4796 4854
Size: 191 12 416 1 431 43 34 58
Cuts at: 4854 5306 5307 5668 2069
Size: 452 1 361 4826
Fragments arranged by size:
4826 598 452 431 416 361 298 258
244 191 168 58 43 34 18 14
12 1 1 1
EcoRI G'AATT_C
Cuts at: 5734 6112 5734
Size: 378 8047
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3202 3202
Size: 8425
FspI TGC'GCA
Cuts at: 2427 2469 2687 5552 6931 2427
Size: 42 218 2865 1379 3921
Fragments arranged by size:
3921 2865 1379 218 42
FspAI rTGC'GCAy
Cuts at: 2427 2469 2427
Size: 42 8383
HaeII r_GCGC'y
Cuts at: 1678 2948 2955 3701 3783 5471 5646 7572 1678
Size: 1270 7 746 82 1688 175 1926 2531
Fragments arranged by size:
2531 1926 1688 1270 746 175 82 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2332 2364 3323 3355 3934
Size: 32 135 32 760 32 959 32 579
Cuts at: 3934 3966 4639 4671 7066 7098 7140 7172 1373
Size: 32 673 32 2395 32 42 32 2626
Fragments arranged by size:
2626 2395 959 760 673 579 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3477 3516 4129 4309 5769 6015
Size: 988 281 1539 39 613 180 1460 246
Cuts at: 6015 669
Size: 3079
Fragments arranged by size:
3079 1539 1460 988 613 281 246 180
39
HindIII A'AGCT_T
Cuts at: 748 4900 748
Size: 4152 4273
HpaI GTT'AAC
Cuts at: 6015 6015
Size: 8425
KasI G'GCGC_C
Cuts at: 3779 5467 3779
Size: 1688 6737
KpnI G_GTAC'C
Cuts at: 5750 5750
Size: 8425
MfeI C'AATT_G
Cuts at: 6024 6024
Size: 8425
MluI A'CGCG_T
Cuts at: 8301 8301
Size: 8425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2040 4586 7539 7723 1305
Size: 735 2546 2953 184 2007
Fragments arranged by size:
2953 2546 2007 735 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4145 5603 10
Size: 54 1425 343 2313 1458 2832
Fragments arranged by size:
2832 2313 1458 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2261 3944 4222 5085 6383 6742 6901
Size: 648 1095 1683 278 863 1298 359 159
Cuts at: 6901 518
Size: 2042
Fragments arranged by size:
2042 1683 1298 1095 863 648 359 278
159
MspA1I CmG'CkG
Cuts at: 1606 2050 2243 2294 2328 3349 3433 3490 3496
Size: 444 193 51 34 1021 84 57 6
Cuts at: 3496 3540 3640 4746 4848 4959 5446 5841 6518
Size: 44 100 1106 102 111 487 395 677
Cuts at: 6518 7666 7911 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1106 1021 677 487 444 395
245 193 111 102 100 84 57 51
44 34 6
NaeI GCC'GGC
Cuts at: 5466 5558 5466
Size: 92 8333
NarI GG'CG_CC
Cuts at: 3780 5468 3780
Size: 1688 6737
NcoI C'CATG_G
Cuts at: 513 1065 2004 3099 513
Size: 552 939 1095 5839
Fragments arranged by size:
5839 1095 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8425
NgoMIV G'CCGG_C
Cuts at: 5464 5556 5464
Size: 92 8333
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8425
NotI GC'GGCC_GC
Cuts at: 5872 5872
Size: 8425
NspI r_CATG'y
Cuts at: 1132 1229 3304 3592 5783 7328 1132
Size: 97 2075 288 2191 1545 2229
Fragments arranged by size:
2229 2191 2075 1545 288 97
PasI CC'CwG_GG
Cuts at: 1348 2152 3318 3664 1348
Size: 804 1166 346 6109
Fragments arranged by size:
6109 1166 804 346
PciI A'CATG_T
Cuts at: 7324 7324
Size: 8425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4633 1108
Size: 3525 4900
PfoI T'CCnGG_A
Cuts at: 2056 2751 2904 3846 4158 4488 4581 4869 2056
Size: 695 153 942 312 330 93 288 5612
Fragments arranged by size:
5612 942 695 330 312 288 153 93
PmeI GTTT'AAAC
Cuts at: 5729 5729
Size: 8425
PmlI CAC'GTG
Cuts at: 5368 5368
Size: 8425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4412 4444 6494 6526 8007 8039 4412
Size: 32 2050 32 1481 32 4798
Fragments arranged by size:
4798 2050 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2500 3070 3859 3871 4719 4762 2500
Size: 570 789 12 848 43 6163
Fragments arranged by size:
6163 848 789 570 43 12
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8425
PsiI TTA'TAA
Cuts at: 5995 5995
Size: 8425
PspOMI G'GGCC_C
Cuts at: 2313 2481 2797 3668 4287 4796 4853 5306 5440
Size: 168 316 871 619 509 57 453 134
Cuts at: 5440 2313
Size: 5298
Fragments arranged by size:
5298 871 619 509 453 316 168 134
57
PspXI vC'TCGA_Gb
Cuts at: 3191 3191
Size: 8425
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2441 2473 2441
Size: 32 8393
PstI C_TGCA'G
Cuts at: 830 3348 3858 3867 3975 4281 4646 4662 4713
Size: 2518 510 9 108 306 365 16 51
Cuts at: 4713 4890 5777 830
Size: 177 887 3478
Fragments arranged by size:
3478 2518 887 510 365 306 177 108
51 16 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8425
PvuII CAG'CTG
Cuts at: 2050 2243 2328 3349 3433 3496 3640 4848 2050
Size: 193 85 1021 84 63 144 1208 5627
Fragments arranged by size:
5627 1208 1021 193 144 85 84 63
SacI G_AGCT'C
Cuts at: 2516 2516
Size: 8425
SacII CC_GC'GG
Cuts at: 3541 3541
Size: 8425
SalI G'TCGA_C
Cuts at: 1936 5767 1936
Size: 3831 4594
SanDI GG'GwC_CC
Cuts at: 4719 4719
Size: 8425
SapI GCTCTTCn'nnn_
Cuts at: 4199 4199
Size: 8425
SbfI CC_TGCA'GG
Cuts at: 4713 5777 4713
Size: 1064 7361
ScaI AGT'ACT
Cuts at: 6673 8385 6673
Size: 1712 6713
SexAI A'CCwGG_T
Cuts at: 3411 3411
Size: 8425
SfcI C'TryA_G
Cuts at: 826 1045 2533 3344 3854 3863 3971 4277 4603
Size: 219 1488 811 510 9 108 306 326
Cuts at: 4603 4642 4658 4709 4886 5773 6908 7589 7780
Size: 39 16 51 177 887 1135 681 191
Cuts at: 7780 826
Size: 1471
Fragments arranged by size:
1488 1471 1135 887 811 681 510 326
306 219 191 177 108 51 39 16
9
SfoI GGC'GCC
Cuts at: 3781 5469 3781
Size: 1688 6737
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8425
SmaI CCC'GGG
Cuts at: 1872 2437 4265 4801 4859 5312 5439 5752 1872
Size: 565 1828 536 58 453 127 313 4545
Fragments arranged by size:
4545 1828 565 536 453 313 127 58
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3191 3200 3951 6529 7430 7692
Size: 197 926 1248 9 751 2578 901 262
Cuts at: 7692 820
Size: 1553
Fragments arranged by size:
2578 1553 1248 926 901 751 262 197
9
SnaBI TAC'GTA
Cuts at: 493 6216 493
Size: 5723 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8425
SphI G_CATG'C
Cuts at: 1132 5783 1132
Size: 4651 3774
SrfI GCCC'GGGC
Cuts at: 4801 4801
Size: 8425
SspI AAT'ATT
Cuts at: 5 52 6349 5
Size: 47 6297 2081
Fragments arranged by size:
6297 2081 47
StuI AGG'CCT
Cuts at: 1517 3167 3791 1517
Size: 1650 624 6151
Fragments arranged by size:
6151 1650 624
StyI C'CwwG_G
Cuts at: 513 1065 2004 2503 2649 2940 3099 3558 5864
Size: 552 939 499 146 291 159 459 2306
Cuts at: 5864 513
Size: 3074
Fragments arranged by size:
3074 2306 939 552 499 459 291 159
146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2263 4640 5113 5536 6452 6469 6622
Size: 1255 683 2377 473 423 916 17 153
Cuts at: 6622 6807 7252 325
Size: 185 445 1498
Fragments arranged by size:
2377 1498 1255 916 683 473 445 423
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3114 6671 8383
Size: 274 80 33 51 1443 1137 3557 1712
Cuts at: 8383 96
Size: 138
Fragments arranged by size:
3557 1712 1443 1137 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2195 2227 2503 2535 2582 2614 2990 3022 3828
Size: 32 276 32 47 32 376 32 806
Cuts at: 3828 3860 4333 4365 4543 4575 4823 4855 8385
Size: 32 473 32 178 32 248 32 3530
Cuts at: 8385 8417 2195
Size: 32 2203
Fragments arranged by size:
3530 2203 806 473 376 276 248 178
47 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2402 3230 1316
Size: 1086 828 6511
Fragments arranged by size:
6511 1086 828
XbaI T'CTAG_A
Cuts at: 5761 5761
Size: 8425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2149 2656 4078 4700 5521 2149
Size: 507 1422 622 821 5053
Fragments arranged by size:
5053 1422 821 622 507
XhoI C'TCGA_G
Cuts at: 1943 3191 1943
Size: 1248 7177
XmaI C'CCGG_G
Cuts at: 1870 2435 4263 4799 4857 5310 5437 5750 1870
Size: 565 1828 536 58 453 127 313 4545
Fragments arranged by size:
4545 1828 565 536 453 313 127 58
XmnI GAAnn'nnTTC
Cuts at: 3134 6554 3134
Size: 3420 5005
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2404 2716 5542 6233
Size: 53 83 186 936 870 312 2826 691
Cuts at: 6233 276
Size: 2468
Fragments arranged by size:
2826 2468 936 870 691 312 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI
NruI NsiI PacI RsrII SfiI SwaI