Restriction map for
pFN21AE3522
(Circular) (Six-base) MAPSORT of: pFN21AE3522.seq Check: 1189 from: 1 to: 6841
pFN21AE3522 6841 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4651 278
Size: 53 83 186 936 3115 2468
Fragments arranged by size:
3115 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2444 2669 4184 1937
Size: 507 225 1515 4594
Fragments arranged by size:
4594 1515 507 225
Acc65I G'GTAC_C
Cuts at: 3332 4162 3332
Size: 830 6011
AclI AA'CG_TT
Cuts at: 1563 4968 5341 1563
Size: 3405 373 3063
Fragments arranged by size:
3405 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2309 2852 3606 3957 4904 6288 6556 1632
Size: 677 543 754 351 947 1384 268 1917
Fragments arranged by size:
1917 1384 947 754 677 543 351 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6644
AflIII A'CryG_T
Cuts at: 1203 2029 3437 5740 6717 1203
Size: 826 1408 2303 977 1327
Fragments arranged by size:
2303 1408 1327 977 826
AgeI A'CCGG_T
Cuts at: 5672 5672
Size: 6841
AhdI GACnn_n'nnGTC
Cuts at: 5570 5570
Size: 6841
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6841
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3933 1184
Size: 2749 4092
AlwNI CAG_nnn'CTG
Cuts at: 3584 4584 6156 3584
Size: 1000 1572 4269
Fragments arranged by size:
4269 1572 1000
ApaLI G'TGCA_C
Cuts at: 3982 4898 6054 3982
Size: 916 1156 4769
Fragments arranged by size:
4769 1156 916
ApoI r'AATT_y
Cuts at: 1493 2833 2857 3281 3572 4150 4376 4528 6637
Size: 1340 24 424 291 578 226 152 2109
Cuts at: 6637 1493
Size: 1697
Fragments arranged by size:
2109 1697 1340 578 424 291 226 152
24
AseI AT'TA_AT
Cuts at: 160 1032 5395 160
Size: 872 4363 1606
Fragments arranged by size:
4363 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6841
AvaI C'yCGr_G
Cuts at: 1870 1943 4166 1870
Size: 73 2223 4545
Fragments arranged by size:
4545 2223 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2979 3012 2979
Size: 33 6808
BamHI G'GATC_C
Cuts at: 4171 4538 4171
Size: 367 6474
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2408 2698 3332 4162 5617
Size: 325 217 627 621 290 634 830 1455
Cuts at: 5617 618
Size: 1842
Fragments arranged by size:
1842 1455 830 634 627 621 325 290
217
BanII G_rGCy'C
Cuts at: 1391 2661 3810 4080 4160 1391
Size: 1270 1149 270 80 4072
Fragments arranged by size:
4072 1270 1149 270 80
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2960 4113 928
Size: 971 1061 1153 3656
Fragments arranged by size:
3656 1153 1061 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5032 5066 1903
Size: 34 50 34 3011 34 3678
Fragments arranged by size:
3678 3011 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2011 2463 4733 5943 2011
Size: 452 2270 1210 2909
Fragments arranged by size:
2909 2270 1210 452
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6841
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5452 136
Size: 107 122 71 631 605 3780 1525
Fragments arranged by size:
3780 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2960 6836 2960
Size: 3876 2965
BlpI GC'TnA_GC
Cuts at: 4258 4258
Size: 6841
Bme1580I G_kGCm'C
Cuts at: 1790 2413 3912 3986 4902 6058 1790
Size: 623 1499 74 916 1156 2573
Fragments arranged by size:
2573 1499 1156 916 623 74
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6841
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4572 5530 442
Size: 416 53 83 399 24 3155 958 1753
Fragments arranged by size:
3155 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2012 2949 1056
Size: 956 937 4948
Fragments arranged by size:
4948 956 937
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2301 3028 4056 5501 1239
Size: 1062 727 1028 1445 2579
Fragments arranged by size:
2579 1445 1062 1028 727
Bpu10I CC'TnA_GC
Cuts at: 2129 3276 4136 2129
Size: 1147 860 4834
Fragments arranged by size:
4834 1147 860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2037 2162 3993 4966 5831 6129 2037
Size: 125 1831 973 865 298 2749
Fragments arranged by size:
2749 1831 973 865 298 125
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2277 3208 3818 5504 882
Size: 617 778 931 610 1686 2219
Fragments arranged by size:
2219 1686 931 778 617 610
BsaAI yAC'GTr
Cuts at: 493 1204 2022 3610 4632 493
Size: 711 818 1588 1022 2702
Fragments arranged by size:
2702 1588 1022 818 711
BsaBI GATnn'nnATC
Cuts at: 3901 6835 3901
Size: 2934 3907
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4648 5030 5665 275
Size: 53 83 186 936 3115 382 635 1451
Fragments arranged by size:
3115 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5274 5672 5946 6093 6504 5274
Size: 398 274 147 411 5611
Fragments arranged by size:
5611 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6616
Fragments arranged by size:
6616 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2773 1186
Size: 1587 5254
BseYI C'CCAG_C
Cuts at: 3540 6044 6540 3540
Size: 2504 496 3841
Fragments arranged by size:
3841 2504 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2323 3704 1163
Size: 573 587 1381 4300
Fragments arranged by size:
4300 1381 587 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2109 4291 5052 6080 1540
Size: 275 186 108 2182 761 1028 2301
Fragments arranged by size:
2301 2182 1028 761 275 186 108
BsiHKAI G_wGCw'C
Cuts at: 3986 4080 4902 4987 6058 3986
Size: 94 822 85 1071 4769
Fragments arranged by size:
4769 1071 822 94 85
BsmI GAATG_Cn'
Cuts at: 3854 4352 4445 3854
Size: 498 93 6250
Fragments arranged by size:
6250 498 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2413 2661 3810 3912 3986 4080 4160
Size: 399 623 248 1149 102 74 94 80
Cuts at: 4160 4902 4987 6058 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1149 1071 742 623 399 248 102
94 85 80 74
BspEI T'CCGG_A
Cuts at: 6504 6504
Size: 6841
BspHI T'CATG_A
Cuts at: 3151 4730 6460 3151
Size: 1579 1730 3532
Fragments arranged by size:
3532 1730 1579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4196 844
Size: 3352 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4728 1394
Size: 429 2905 3507
Fragments arranged by size:
3507 2905 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2019 3967 5336 5510 65
Size: 1183 771 1948 1369 174 1396
Fragments arranged by size:
1948 1396 1369 1183 771 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5485 5672 1700
Size: 110 3675 187 2869
Fragments arranged by size:
3675 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6841
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6841
BssSI C'ACGA_G
Cuts at: 4901 5913 4901
Size: 1012 5829
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6841
BstBI TT'CG_AA
Cuts at: 2491 5645 2491
Size: 3154 3687
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6841
BstYI r'GATC_y
Cuts at: 1972 2960 4171 4538 4923 4940 6392 6478 6490
Size: 988 1211 367 385 17 1452 86 12
Cuts at: 6490 6836 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1211 988 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2445 2445
Size: 6841
Bsu36I CC'TnA_GG
Cuts at: 3705 3705
Size: 6841
BtgI C'CryG_G
Cuts at: 513 1065 2370 3870 3886 513
Size: 552 1305 1500 16 3468
Fragments arranged by size:
3468 1500 1305 552 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6102
Fragments arranged by size:
6102 683 56
BtsI GCAGTG_nn'
Cuts at: 2335 3526 3915 3955 4357 5151 5171 2335
Size: 1191 389 40 402 794 20 4005
Fragments arranged by size:
4005 1191 794 402 389 40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6841
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3161 3196 529
Size: 35 2597 35 4174
Fragments arranged by size:
4174 2597 35 35
DraI TTT'AAA
Cuts at: 2910 4145 2910
Size: 1235 5606
DraIII CAC_nnn'GTG
Cuts at: 2092 3229 2092
Size: 1137 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3054 5848 809
Size: 507 1738 2794 1802
Fragments arranged by size:
2794 1802 1738 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3567 4288 5177
Size: 54 1425 50 275 18 1737 721 889
Cuts at: 5177 6806 8
Size: 1629 43
Fragments arranged by size:
1737 1629 1425 889 721 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4288 1537
Size: 275 2476 4090
Fragments arranged by size:
4090 2476 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2160 4771 1353
Size: 807 2611 3423
Fragments arranged by size:
3423 2611 807
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5428 5802 5948 387
Size: 1064 138 3839 374 146 1280
Fragments arranged by size:
3839 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4078 4078
Size: 6841
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 2309 2852 3028 3606 3957 4056
Size: 393 669 8 543 176 578 351 99
Cuts at: 4056 4904 5501 6288 6556 1239
Size: 848 597 787 268 1524
Fragments arranged by size:
1524 848 787 669 597 578 543 393
351 268 176 99 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3704 3704
Size: 6841
EcoRI G'AATT_C
Cuts at: 2857 4150 4528 2857
Size: 1293 378 5170
Fragments arranged by size:
5170 1293 378
FspI TGC'GCA
Cuts at: 5347 5347
Size: 6841
HaeII r_GCGC'y
Cuts at: 1678 5988 1678
Size: 4310 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3443 3475 3708 3740 4081
Size: 32 135 32 1871 32 233 32 341
Cuts at: 4081 4106 4113 4138 5482 5514 5556 5588 1373
Size: 25 7 25 1344 32 42 32 2626
Fragments arranged by size:
2626 1871 1344 341 233 135 42 32
32 32 32 32 32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2670 4185 4431 669
Size: 988 281 732 1515 246 3079
Fragments arranged by size:
3079 1515 988 732 281 246
HindIII A'AGCT_T
Cuts at: 748 2912 3588 4011 748
Size: 2164 676 423 3578
Fragments arranged by size:
3578 2164 676 423
HpaI GTT'AAC
Cuts at: 4431 4431
Size: 6841
KpnI G_GTAC'C
Cuts at: 3336 4166 3336
Size: 830 6011
MfeI C'AATT_G
Cuts at: 4440 4440
Size: 6841
MluI A'CGCG_T
Cuts at: 6717 6717
Size: 6841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5955 6139 1305
Size: 4650 184 2007
Fragments arranged by size:
4650 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3569 10
Size: 54 1425 343 1737 3282
Fragments arranged by size:
3282 1737 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2406 3960 4101 4799 5158 5317 518
Size: 648 1240 1554 141 698 359 159 2042
Fragments arranged by size:
2042 1554 1240 698 648 359 159 141
MspA1I CmG'CkG
Cuts at: 1606 2041 3872 4257 4934 6082 6327 1606
Size: 435 1831 385 677 1148 245 2120
Fragments arranged by size:
2120 1831 1148 677 435 385 245
NcoI C'CATG_G
Cuts at: 513 1065 2370 3886 513
Size: 552 1305 1516 3468
Fragments arranged by size:
3468 1516 1305 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6841
NheI G'CTAG_C
Cuts at: 1052 2008 2945 1052
Size: 956 937 4948
Fragments arranged by size:
4948 956 937
NotI GC'GGCC_GC
Cuts at: 4288 4288
Size: 6841
NspI r_CATG'y
Cuts at: 1132 1229 2033 2174 3441 3450 3982 4199 5744
Size: 97 804 141 1267 9 532 217 1545
Cuts at: 5744 1132
Size: 2229
Fragments arranged by size:
2229 1545 1267 804 532 217 141 97
9
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6841
PciI A'CATG_T
Cuts at: 2029 3437 5740 2029
Size: 1408 2303 3130
Fragments arranged by size:
3130 2303 1408
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2932 1108
Size: 1824 5017
PmeI GTTT'AAAC
Cuts at: 4145 4145
Size: 6841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4910 4942 6423 6455 4910
Size: 32 1481 32 5296
Fragments arranged by size:
5296 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6841
PsiI TTA'TAA
Cuts at: 4411 4411
Size: 6841
PstI C_TGCA'G
Cuts at: 830 2046 3821 4193 830
Size: 1216 1775 372 3478
Fragments arranged by size:
3478 1775 1216 372
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6841
PvuII CAG'CTG
Cuts at: 2041 2041
Size: 6841
SacI G_AGCT'C
Cuts at: 4080 4080
Size: 6841
SacII CC_GC'GG
Cuts at: 3873 3873
Size: 6841
SalI G'TCGA_C
Cuts at: 1936 2668 4183 1936
Size: 732 1515 4594
Fragments arranged by size:
4594 1515 732
SapI GCTCTTCn'nnn_
Cuts at: 2160 2160
Size: 6841
SbfI CC_TGCA'GG
Cuts at: 4193 4193
Size: 6841
ScaI AGT'ACT
Cuts at: 2739 5089 6801 2739
Size: 2350 1712 2779
Fragments arranged by size:
2779 2350 1712
SfcI C'TryA_G
Cuts at: 826 1045 2042 3817 4189 5324 6005 6196 826
Size: 219 997 1775 372 1135 681 191 1471
Fragments arranged by size:
1775 1471 1135 997 681 372 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6841
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6841
SmaI CCC'GGG
Cuts at: 1872 4168 1872
Size: 2296 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2052 2177 4008 4945 5846 6108
Size: 197 926 109 125 1831 937 901 262
Cuts at: 6108 820
Size: 1553
Fragments arranged by size:
1831 1553 937 926 901 262 197 125
109
SnaBI TAC'GTA
Cuts at: 493 3610 4632 493
Size: 3117 1022 2702
Fragments arranged by size:
3117 2702 1022
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6841
SphI G_CATG'C
Cuts at: 1132 2174 4199 1132
Size: 1042 2025 3774
Fragments arranged by size:
3774 2025 1042
SspI AAT'ATT
Cuts at: 5 52 4765 5
Size: 47 4713 2081
Fragments arranged by size:
4713 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6841
StyI C'CwwG_G
Cuts at: 513 1065 2370 3886 4280 513
Size: 552 1305 1516 394 3074
Fragments arranged by size:
3074 1516 1305 552 394
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 2527 4868 4885 5038 5223 5668
Size: 1255 543 404 2341 17 153 185 445
Cuts at: 5668 325
Size: 1498
Fragments arranged by size:
2341 1498 1255 543 445 404 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2737 3135 3661
Size: 274 80 33 51 1443 760 398 526
Cuts at: 3661 5087 6799 96
Size: 1426 1712 138
Fragments arranged by size:
1712 1443 1426 760 526 398 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6801 6833 6801
Size: 32 6809
Tth111I GACn'n_nGTC
Cuts at: 1316 2110 1316
Size: 794 6047
XbaI T'CTAG_A
Cuts at: 4177 4177
Size: 6841
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2147 2011
Size: 136 6705
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6841
XmaI C'CCGG_G
Cuts at: 1870 4166 1870
Size: 2296 4545
XmnI GAAnn'nnTTC
Cuts at: 2541 3108 3643 4970 2541
Size: 567 535 1327 4412
Fragments arranged by size:
4412 1327 567 535
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4649 276
Size: 53 83 186 936 3115 2468
Fragments arranged by size:
3115 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SanDI SexAI SfiI SfoI SrfI SwaI