Restriction map for
pFN21AE3515
(Circular) (Six-base) MAPSORT of: pFN21AE3515.seq Check: 968 from: 1 to: 7609
pFN21AE3515 7609 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4868 4868
Size: 7609
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5419 278
Size: 53 83 186 936 3883 2468
Fragments arranged by size:
3883 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3045 4952 1937
Size: 1108 1907 4594
Fragments arranged by size:
4594 1907 1108
Acc65I G'GTAC_C
Cuts at: 4930 4930
Size: 7609
AclI AA'CG_TT
Cuts at: 1563 5736 6109 1563
Size: 4173 373 3063
Fragments arranged by size:
4173 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2928 3865 4080 4649 5672 7056 7324 1632
Size: 1296 937 215 569 1023 1384 268 1917
Fragments arranged by size:
1917 1384 1296 1023 937 569 268 215
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7412
AflIII A'CryG_T
Cuts at: 1203 6508 7485 1203
Size: 5305 977 1327
Fragments arranged by size:
5305 1327 977
AgeI A'CCGG_T
Cuts at: 6440 6440
Size: 7609
AhdI GACnn_n'nnGTC
Cuts at: 6338 6338
Size: 7609
AleI CACnn'nnGTG
Cuts at: 1166 2786 4799 1166
Size: 1620 2013 3976
Fragments arranged by size:
3976 2013 1620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7609
AlwNI CAG_nnn'CTG
Cuts at: 3619 5352 6924 3619
Size: 1733 1572 4304
Fragments arranged by size:
4304 1733 1572
ApaI G_GGCC'C
Cuts at: 2489 3084 2489
Size: 595 7014
ApaLI G'TGCA_C
Cuts at: 5666 6822 5666
Size: 1156 6453
ApoI r'AATT_y
Cuts at: 1493 3335 3486 3728 4918 5144 5296 7405 1493
Size: 1842 151 242 1190 226 152 2109 1697
Fragments arranged by size:
2109 1842 1697 1190 242 226 152 151
AseI AT'TA_AT
Cuts at: 160 1032 6163 160
Size: 872 5131 1606
Fragments arranged by size:
5131 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7609
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2080 2131 2362 2452 2559 4934
Size: 73 96 41 51 231 90 107 2375
Cuts at: 4934 1870
Size: 4545
Fragments arranged by size:
4545 2375 231 107 96 90 73 51
41
BamHI G'GATC_C
Cuts at: 2990 4939 5306 2990
Size: 1949 367 5293
Fragments arranged by size:
5293 1949 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2114 2358 2466 4930 6385
Size: 325 217 627 327 244 108 2464 1455
Cuts at: 6385 618
Size: 1842
Fragments arranged by size:
2464 1842 1455 627 327 325 244 217
108
BanII G_rGCy'C
Cuts at: 1391 2402 2489 2735 3073 3084 4928 1391
Size: 1011 87 246 338 11 1844 4072
Fragments arranged by size:
4072 1844 1011 338 246 87 11
BbeI G_GCGC'C
Cuts at: 2118 2362 2470 2118
Size: 244 108 7257
Fragments arranged by size:
7257 244 108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2817 2841 928
Size: 971 918 24 5696
Fragments arranged by size:
5696 971 918 24
BbvCI CC'TCA_GC
Cuts at: 2031 2031
Size: 7609
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3853 3887 5800 5834 1903
Size: 34 1916 34 1913 34 3678
Fragments arranged by size:
3678 1916 1913 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5501 6711 5501
Size: 1210 6399
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7609
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2390 2508 6220
Size: 107 122 71 631 605 718 118 3712
Cuts at: 6220 136
Size: 1525
Fragments arranged by size:
3712 1525 718 631 605 122 118 107
71
BglII A'GATC_T
Cuts at: 4108 4234 7604 4108
Size: 126 3370 4113
Fragments arranged by size:
4113 3370 126
BlpI GC'TnA_GC
Cuts at: 5026 5026
Size: 7609
Bme1580I G_kGCm'C
Cuts at: 1790 2489 2498 3084 5670 6826 1790
Size: 699 9 586 2586 1156 2573
Fragments arranged by size:
2586 2573 1156 699 586 9
BmgBI CAC'GTC
Cuts at: 1318 4397 1318
Size: 3079 4530
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2498 3151 5340
Size: 416 53 83 399 24 1081 653 2189
Cuts at: 5340 6298 442
Size: 958 1753
Fragments arranged by size:
2189 1753 1081 958 653 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3228 1056
Size: 2172 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1999 2180 4459 6269 1239
Size: 760 181 2279 1810 2579
Fragments arranged by size:
2579 2279 1810 760 181
Bpu10I CC'TnA_GC
Cuts at: 2031 2031
Size: 7609
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3034 3215 5734 6599 6897 3034
Size: 181 2519 865 298 3746
Fragments arranged by size:
3746 2519 865 298 181
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2606 4296 6272 882
Size: 617 1107 1690 1976 2219
Fragments arranged by size:
2219 1976 1690 1107 617
BsaAI yAC'GTr
Cuts at: 493 1204 4733 4906 5400 493
Size: 711 3529 173 494 2702
Fragments arranged by size:
3529 2702 711 494 173
BsaBI GATnn'nnATC
Cuts at: 4377 7603 4377
Size: 3226 4383
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 2115 2359 2467
Size: 53 83 186 936 513 69 244 108
Cuts at: 2467 4587 5416 5798 6433 275
Size: 2120 829 382 635 1451
Fragments arranged by size:
2120 1451 936 829 635 513 382 244
186 108 83 69 53
BsaWI w'CCGG_w
Cuts at: 4545 6042 6440 6714 6861 7272 4545
Size: 1497 398 274 147 411 4882
Fragments arranged by size:
4882 1497 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2958 2988 1178
Size: 30 165 30 1555 30 5799
Fragments arranged by size:
5799 1555 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2223 2309 2312 2315 2318 2321 2377 4215
Size: 1037 86 3 3 3 3 56 1838
Cuts at: 4215 4457 4648 1186
Size: 242 191 4147
Fragments arranged by size:
4147 1838 1037 242 191 86 56 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2498 2894 3340 4645 4684 4749 6812 7308 2498
Size: 396 446 1305 39 65 2063 496 2799
Fragments arranged by size:
2799 2063 1305 496 446 396 65 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2045 3198 4896 1163
Size: 573 309 1153 1698 3876
Fragments arranged by size:
3876 1698 1153 573 309
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2057 2109 2193 2211 2382 2394
Size: 275 186 56 52 84 18 171 12
Cuts at: 2394 5059 5820 6848 1540
Size: 2665 761 1028 2301
Fragments arranged by size:
2665 2301 1028 761 275 186 171 84
56 52 18 12
BsiHKAI G_wGCw'C
Cuts at: 2252 2402 2735 5670 5755 6826 2252
Size: 150 333 2935 85 1071 3035
Fragments arranged by size:
3035 2935 1071 333 150 85
BsmI GAATG_Cn'
Cuts at: 3492 4555 5120 5213 3492
Size: 1063 565 93 5888
Fragments arranged by size:
5888 1063 565 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4335 4528 4335
Size: 193 7416
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2252 2402 2489 2498 2735 3073 3084
Size: 399 462 150 87 9 237 338 11
Cuts at: 3084 4928 5670 5755 6826 1391
Size: 1844 742 85 1071 2174
Fragments arranged by size:
2174 1844 1071 742 462 399 338 237
150 87 85 11 9
BspEI T'CCGG_A
Cuts at: 7272 7272
Size: 7609
BspHI T'CATG_A
Cuts at: 5498 7228 5498
Size: 1730 5879
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2952 4868 4964 844
Size: 2108 1916 96 3489
Fragments arranged by size:
3489 2108 1916 96
BsrBI CCG'CTC
Cuts at: 1394 1823 2239 2457 5496 1394
Size: 429 416 218 3039 3507
Fragments arranged by size:
3507 3039 429 416 218
BsrDI GCAATG_nn'
Cuts at: 65 1248 3150 6104 6278 65
Size: 1183 1902 2954 174 1396
Fragments arranged by size:
2954 1902 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 2469 6253 6440 1700
Size: 110 275 384 3784 187 2869
Fragments arranged by size:
3784 2869 384 275 187 110
BsrGI T'GTAC_A
Cuts at: 96 3288 96
Size: 3192 4417
BssHII G'CGCG_C
Cuts at: 1925 2070 2409 1925
Size: 145 339 7125
Fragments arranged by size:
7125 339 145
BssSI C'ACGA_G
Cuts at: 5669 6681 5669
Size: 1012 6597
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3221 794
Size: 2427 5182
BstBI TT'CG_AA
Cuts at: 6413 6413
Size: 7609
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7609
BstYI r'GATC_y
Cuts at: 1972 2990 3102 4108 4234 4939 5306 5691 5708
Size: 1018 112 1006 126 705 367 385 17
Cuts at: 5708 7160 7246 7258 7604 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1018 1006 705 385 367 346
126 112 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2109 2217 2403 2437 2829 4167
Size: 552 939 105 108 186 34 392 1338
Cuts at: 4167 513
Size: 3955
Fragments arranged by size:
3955 1338 939 552 392 186 108 105
34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2513 487
Size: 683 56 1287 5583
Fragments arranged by size:
5583 1287 683 56
BtsI GCAGTG_nn'
Cuts at: 5125 5919 5939 5125
Size: 794 20 6795
Fragments arranged by size:
6795 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7609
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7574
DraI TTT'AAA
Cuts at: 2815 3384 4913 2815
Size: 569 1529 5511
Fragments arranged by size:
5511 1529 569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3772 4812 6616 809
Size: 507 2456 1040 1804 1802
Fragments arranged by size:
2456 1804 1802 1040 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2054 2106 2190
Size: 54 1425 50 275 18 224 52 84
Cuts at: 2190 2208 2379 2391 4668 5056 5945 7574 8
Size: 18 171 12 2277 388 889 1629 43
Fragments arranged by size:
2277 1629 1425 889 388 275 224 171
84 54 52 50 43 18 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2054 2106 2190 2208 2379 2391 5056
Size: 275 242 52 84 18 171 12 2665
Cuts at: 5056 1537
Size: 4090
Fragments arranged by size:
4090 2665 275 242 171 84 52 18
12
EarI CTCTTCn'nnn_
Cuts at: 1353 2777 3468 3728 4509 4625 4731 5539 1353
Size: 1424 691 260 781 116 106 808 3423
Fragments arranged by size:
3423 1424 808 781 691 260 116 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2343 2588 6196 6570 6716 387
Size: 1064 138 754 245 3608 374 146 1280
Fragments arranged by size:
3608 1280 1064 754 374 245 146 138
EcoICRI GAG'CTC
Cuts at: 2400 2733 2400
Size: 333 7276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2180 2928 3865 4080 4459 4649
Size: 393 367 181 748 937 215 379 190
Cuts at: 4649 5672 6269 7056 7324 1239
Size: 1023 597 787 268 1524
Fragments arranged by size:
1524 1023 937 787 748 597 393 379
367 268 215 190 181
EcoO109I rG'GnC_Cy
Cuts at: 2155 2200 2485 2518 3081 4366 2155
Size: 45 285 33 563 1285 5398
Fragments arranged by size:
5398 1285 563 285 45 33
EcoRI G'AATT_C
Cuts at: 3335 4918 5296 3335
Size: 1583 378 5648
Fragments arranged by size:
5648 1583 378
EcoRV GAT'ATC
Cuts at: 3934 3934
Size: 7609
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4231 4231
Size: 7609
FspI TGC'GCA
Cuts at: 6115 6115
Size: 7609
HaeII r_GCGC'y
Cuts at: 1678 2118 2362 2470 6756 1678
Size: 440 244 108 4286 2531
Fragments arranged by size:
4286 2531 440 244 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3032 3064 4040 4072 4221
Size: 32 135 32 1460 32 976 32 149
Cuts at: 4221 4253 6250 6282 6324 6356 1373
Size: 32 1997 32 42 32 2626
Fragments arranged by size:
2626 1997 1460 976 149 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4817 4953 5199 669
Size: 988 281 2879 136 246 3079
Fragments arranged by size:
3079 2879 988 281 246 136
HindIII A'AGCT_T
Cuts at: 748 4134 748
Size: 3386 4223
HpaI GTT'AAC
Cuts at: 5199 5199
Size: 7609
KasI G'GCGC_C
Cuts at: 2114 2358 2466 2114
Size: 244 108 7257
Fragments arranged by size:
7257 244 108
KpnI G_GTAC'C
Cuts at: 4934 4934
Size: 7609
MfeI C'AATT_G
Cuts at: 5208 5208
Size: 7609
MluI A'CGCG_T
Cuts at: 7485 7485
Size: 7609
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4638 4722 6723 6907 1305
Size: 3333 84 2001 184 2007
Fragments arranged by size:
3333 2007 2001 184 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5787
Fragments arranged by size:
5787 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2756 2786 4257 4799 5567 5926 6085
Size: 648 1590 30 1471 542 768 359 159
Cuts at: 6085 518
Size: 2042
Fragments arranged by size:
2042 1590 1471 768 648 542 359 159
30
MspA1I CmG'CkG
Cuts at: 1606 2152 2219 2524 3212 5025 5702 6850 7095
Size: 546 67 305 688 1813 677 1148 245
Cuts at: 7095 1606
Size: 2120
Fragments arranged by size:
2120 1813 1148 688 677 546 305 245
67
NaeI GCC'GGC
Cuts at: 2087 2471 2087
Size: 384 7225
NarI GG'CG_CC
Cuts at: 2115 2359 2467 2115
Size: 244 108 7257
Fragments arranged by size:
7257 244 108
NcoI C'CATG_G
Cuts at: 513 1065 2004 2829 513
Size: 552 939 825 5293
Fragments arranged by size:
5293 939 825 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7609
NgoMIV G'CCGG_C
Cuts at: 2085 2469 2085
Size: 384 7225
NheI G'CTAG_C
Cuts at: 1052 3224 1052
Size: 2172 5437
NotI GC'GGCC_GC
Cuts at: 2054 2190 5056 2054
Size: 136 2866 4607
Fragments arranged by size:
4607 2866 136
NspI r_CATG'y
Cuts at: 1132 1229 4967 6512 1132
Size: 97 3738 1545 2229
Fragments arranged by size:
3738 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7609
PciI A'CATG_T
Cuts at: 6508 6508
Size: 7609
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7609
PmeI GTTT'AAAC
Cuts at: 4913 4913
Size: 7609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5678 5710 7191 7223 5678
Size: 32 1481 32 6064
Fragments arranged by size:
6064 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2200 2518 4366 2200
Size: 318 1848 5443
Fragments arranged by size:
5443 1848 318
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7609
PsiI TTA'TAA
Cuts at: 5179 5179
Size: 7609
PspOMI G'GGCC_C
Cuts at: 2485 3080 2485
Size: 595 7014
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4713 4745 4713
Size: 32 7577
PstI C_TGCA'G
Cuts at: 830 4961 830
Size: 4131 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7609
PvuII CAG'CTG
Cuts at: 3212 3212
Size: 7609
SacI G_AGCT'C
Cuts at: 2402 2735 2402
Size: 333 7276
SacII CC_GC'GG
Cuts at: 2220 2220
Size: 7609
SalI G'TCGA_C
Cuts at: 1936 4951 1936
Size: 3015 4594
SanDI GG'GwC_CC
Cuts at: 2200 2518 2200
Size: 318 7291
SapI GCTCTTCn'nnn_
Cuts at: 2777 3728 2777
Size: 951 6658
SbfI CC_TGCA'GG
Cuts at: 4961 4961
Size: 7609
ScaI AGT'ACT
Cuts at: 3886 5857 7569 3886
Size: 1971 1712 3926
Fragments arranged by size:
3926 1971 1712
SfcI C'TryA_G
Cuts at: 826 1045 3567 3611 4857 4957 6092 6773 6964
Size: 219 2522 44 1246 100 1135 681 191
Cuts at: 6964 826
Size: 1471
Fragments arranged by size:
2522 1471 1246 1135 681 219 191 100
44
SfoI GGC'GCC
Cuts at: 2116 2360 2468 2116
Size: 244 108 7257
Fragments arranged by size:
7257 244 108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7609
SgrAI Cr'CCGG_yG
Cuts at: 1700 2469 1700
Size: 769 6840
SmaI CCC'GGG
Cuts at: 1872 2041 2082 2133 2364 4936 1872
Size: 169 41 51 231 2572 4545
Fragments arranged by size:
4545 2572 231 169 51 41
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3013 3230 5713 6614 6876 820
Size: 197 926 1070 217 2483 901 262 1553
Fragments arranged by size:
2483 1553 1070 926 901 262 217 197
SnaBI TAC'GTA
Cuts at: 493 5400 493
Size: 4907 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7609
SphI G_CATG'C
Cuts at: 1132 4967 1132
Size: 3835 3774
SrfI GCCC'GGGC
Cuts at: 2364 2364
Size: 7609
SspI AAT'ATT
Cuts at: 5 52 4278 5533 5
Size: 47 4226 1255 2081
Fragments arranged by size:
4226 2081 1255 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7609
StyI C'CwwG_G
Cuts at: 513 1065 2004 2829 5048 513
Size: 552 939 825 2219 3074
Fragments arranged by size:
3074 2219 939 825 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3267 4331 5636 5653 5806 5991 6436
Size: 1255 1687 1064 1305 17 153 185 445
Cuts at: 6436 325
Size: 1498
Fragments arranged by size:
1687 1498 1305 1255 1064 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2742 2877 3288
Size: 274 80 33 51 1443 765 135 411
Cuts at: 3288 3884 5855 7567 96
Size: 596 1971 1712 138
Fragments arranged by size:
1971 1712 1443 765 596 411 274 138
135 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2322 2339 2354 2371 2757 2789 7569 7601 2322
Size: 17 15 17 386 32 4780 32 2330
Fragments arranged by size:
4780 2330 386 32 32 17 17 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7609
XbaI T'CTAG_A
Cuts at: 4945 4945
Size: 7609
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4781 4781
Size: 7609
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7609
XmaI C'CCGG_G
Cuts at: 1870 2039 2080 2131 2362 4934 1870
Size: 169 41 51 231 2572 4545
Fragments arranged by size:
4545 2572 231 169 51 41
XmnI GAAnn'nnTTC
Cuts at: 4492 4518 5738 4492
Size: 26 1220 6363
Fragments arranged by size:
6363 1220 26
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5417 276
Size: 53 83 186 936 3883 2468
Fragments arranged by size:
3883 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NruI
NsiI PacI PfoI PmlI PspXI RsrII SexAI SfiI
SwaI