Restriction map for
pFN21AE3514
(Circular) (Six-base) MAPSORT of: pFN21AE3514.seq Check: 2220 from: 1 to: 5440
pFN21AE3514 5440 bp
With 182 enzymes: SgfI *
March 28, 2012 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2356 2356
Size: 5440
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3250 278
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2783 1937
Size: 846 4594
Acc65I G'GTAC_C
Cuts at: 2761 2761
Size: 5440
AclI AA'CG_TT
Cuts at: 1563 2691 3567 3940 1563
Size: 1128 876 373 3063
Fragments arranged by size:
3063 1128 876 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3503 4887 5155 1632
Size: 1871 1384 268 1917
Fragments arranged by size:
1917 1871 1384 268
AfeI AGC'GCT
Cuts at: 2426 2426
Size: 5440
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5243
AflIII A'CryG_T
Cuts at: 1203 4339 5316 1203
Size: 3136 977 1327
Fragments arranged by size:
3136 1327 977
AgeI A'CCGG_T
Cuts at: 4271 4271
Size: 5440
AhdI GACnn_n'nnGTC
Cuts at: 4169 4169
Size: 5440
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5440
AlwNI CAG_nnn'CTG
Cuts at: 3183 4755 3183
Size: 1572 3868
ApaLI G'TGCA_C
Cuts at: 3497 4653 3497
Size: 1156 4284
ApoI r'AATT_y
Cuts at: 1493 2749 2975 3127 5236 1493
Size: 1256 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3994 160
Size: 872 2962 1606
Fragments arranged by size:
2962 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
AvaI C'yCGr_G
Cuts at: 1870 1943 2765 1870
Size: 73 822 4545
Fragments arranged by size:
4545 822 73
BamHI G'GATC_C
Cuts at: 2770 3137 2770
Size: 367 5073
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2307 2368 2651 2761 4216
Size: 325 217 627 520 61 283 110 1455
Cuts at: 4216 618
Size: 1842
Fragments arranged by size:
1842 1455 627 520 325 283 217 110
61
BanII G_rGCy'C
Cuts at: 1391 2613 2718 2759 1391
Size: 1222 105 41 4072
Fragments arranged by size:
4072 1222 105 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2637 928
Size: 971 738 3731
Fragments arranged by size:
3731 971 738
BbvCI CC'TCA_GC
Cuts at: 2700 2700
Size: 5440
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3631 3665 1903
Size: 34 1694 34 3678
Fragments arranged by size:
3678 1694 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3332 4542 3332
Size: 1210 4230
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5440
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2436 4051 136
Size: 107 122 71 631 605 764 1615 1525
Fragments arranged by size:
1615 1525 764 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5435 5435
Size: 5440
BlpI GC'TnA_GC
Cuts at: 2612 2857 2612
Size: 245 5195
Bme1580I G_kGCm'C
Cuts at: 1790 2310 2656 3501 4657 1790
Size: 520 346 845 1156 2573
Fragments arranged by size:
2573 1156 845 520 346
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5440
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3171 4129 442
Size: 416 53 83 399 24 1754 958 1753
Fragments arranged by size:
1754 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2150 1056
Size: 1094 4346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2527 2645 2666 4100 1239
Size: 1288 118 21 1434 2579
Fragments arranged by size:
2579 1434 1288 118 21
Bpu10I CC'TnA_GC
Cuts at: 2700 2700
Size: 5440
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2069 3565 4430 4728 2069
Size: 1496 865 298 2781
Fragments arranged by size:
2781 1496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4103 882
Size: 617 2604 2219
Fragments arranged by size:
2604 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3231 493
Size: 711 2027 2702
Fragments arranged by size:
2702 2027 711
BsaBI GATnn'nnATC
Cuts at: 5434 5434
Size: 5440
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3247 3629 4264 275
Size: 53 83 186 936 1714 382 635 1451
Fragments arranged by size:
1714 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2139 2220 3873 4271 4545 4692 5103 2139
Size: 81 1653 398 274 147 411 2476
Fragments arranged by size:
2476 1653 411 398 274 147 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5215
Fragments arranged by size:
5215 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2678 1186
Size: 1492 3948
BseYI C'CCAG_C
Cuts at: 2418 4643 5139 2418
Size: 2225 496 2719
Fragments arranged by size:
2719 2225 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2341 2480 1163
Size: 573 605 139 4123
Fragments arranged by size:
4123 605 573 139
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2463 2890 3651 4679 1540
Size: 275 186 462 427 761 1028 2301
Fragments arranged by size:
2301 1028 761 462 427 275 186
BsiHKAI G_wGCw'C
Cuts at: 2066 2394 2473 2613 3501 3586 4657 2066
Size: 328 79 140 888 85 1071 2849
Fragments arranged by size:
2849 1071 888 328 140 85 79
BsmI GAATG_Cn'
Cuts at: 2951 3044 2951
Size: 93 5347
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2310 2394 2473 2613 2656 2718
Size: 399 276 244 84 79 140 43 62
Cuts at: 2718 2759 3501 3586 4657 1391
Size: 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 276 244 140 85
84 79 62 43 41
BspEI T'CCGG_A
Cuts at: 2220 5103 2220
Size: 2883 2557
BspHI T'CATG_A
Cuts at: 3329 5059 3329
Size: 1730 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2356 2795 844
Size: 1512 439 3489
Fragments arranged by size:
3489 1512 439
BsrBI CCG'CTC
Cuts at: 1394 1823 2639 3327 1394
Size: 429 816 688 3507
Fragments arranged by size:
3507 816 688 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2401 3935 4109 65
Size: 1183 1153 1534 174 1396
Fragments arranged by size:
1534 1396 1183 1153 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2394 4084 4271 1700
Size: 110 584 1690 187 2869
Fragments arranged by size:
2869 1690 584 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5440
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5440
BssSI C'ACGA_G
Cuts at: 3500 4512 3500
Size: 1012 4428
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2130 794
Size: 1336 4104
BstBI TT'CG_AA
Cuts at: 4244 4244
Size: 5440
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5440
BstYI r'GATC_y
Cuts at: 1972 2770 3137 3522 3539 4991 5077 5089 5435
Size: 798 367 385 17 1452 86 12 346
Cuts at: 5435 1972
Size: 1977
Fragments arranged by size:
1977 1452 798 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2074 513
Size: 552 939 70 3879
Fragments arranged by size:
3879 939 552 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2282 487
Size: 683 56 1056 3645
Fragments arranged by size:
3645 1056 683 56
BtsI GCAGTG_nn'
Cuts at: 2956 3750 3770 2956
Size: 794 20 4626
Fragments arranged by size:
4626 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5405
DraI TTT'AAA
Cuts at: 2744 2744
Size: 5440
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4447 809
Size: 507 3131 1802
Fragments arranged by size:
3131 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2233 2460 2887
Size: 54 1425 50 275 18 403 227 427
Cuts at: 2887 3776 5405 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 427 403 275 227 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2460 2887 1537
Size: 275 648 427 4090
Fragments arranged by size:
4090 648 427 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 3370 1353
Size: 683 1334 3423
Fragments arranged by size:
3423 1334 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2621 4027 4401 4547 387
Size: 1064 138 1032 1406 374 146 1280
Fragments arranged by size:
1406 1280 1064 1032 374 146 138
EcoICRI GAG'CTC
Cuts at: 2611 2611
Size: 5440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2527 2645 2666 3503 4100 4887 5155
Size: 393 895 118 21 837 597 787 268
Cuts at: 5155 1239
Size: 1524
Fragments arranged by size:
1524 895 837 787 597 393 268 118
21
EcoO109I rG'GnC_Cy
Cuts at: 2379 2415 2379
Size: 36 5404
EcoRI G'AATT_C
Cuts at: 2749 3127 2749
Size: 378 5062
FspI TGC'GCA
Cuts at: 3946 3946
Size: 5440
HaeII r_GCGC'y
Cuts at: 1678 2428 2527 4587 1678
Size: 750 99 2060 2531
Fragments arranged by size:
2531 2060 750 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4081 4113 4155 4187 1373
Size: 32 135 32 2509 32 42 32 2626
Fragments arranged by size:
2626 2509 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2784 3030 669
Size: 988 281 846 246 3079
Fragments arranged by size:
3079 988 846 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5440
HpaI GTT'AAC
Cuts at: 3030 3030
Size: 5440
KpnI G_GTAC'C
Cuts at: 2765 2765
Size: 5440
MfeI C'AATT_G
Cuts at: 3039 3039
Size: 5440
MluI A'CGCG_T
Cuts at: 5316 5316
Size: 5440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4554 4738 1305
Size: 3249 184 2007
Fragments arranged by size:
3249 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3618
Fragments arranged by size:
3618 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3398 3757 3916 518
Size: 648 2232 359 159 2042
Fragments arranged by size:
2232 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2856 3533 4681 4926 1606
Size: 1250 677 1148 245 2120
Fragments arranged by size:
2120 1250 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2074 513
Size: 552 939 70 3879
Fragments arranged by size:
3879 939 552 70
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5440
NheI G'CTAG_C
Cuts at: 1052 2146 1052
Size: 1094 4346
NotI GC'GGCC_GC
Cuts at: 2887 2887
Size: 5440
NspI r_CATG'y
Cuts at: 1132 1229 2286 2798 4343 1132
Size: 97 1057 512 1545 2229
Fragments arranged by size:
2229 1545 1057 512 97
PasI CC'CwG_GG
Cuts at: 1348 2434 2718 1348
Size: 1086 284 4070
Fragments arranged by size:
4070 1086 284
PciI A'CATG_T
Cuts at: 4339 4339
Size: 5440
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5440
PfoI T'CCnGG_A
Cuts at: 2622 2622
Size: 5440
PmeI GTTT'AAAC
Cuts at: 2744 2744
Size: 5440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 5022 5054 3509
Size: 32 1481 32 3895
Fragments arranged by size:
3895 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2379 2415 2379
Size: 36 5404
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5440
PsiI TTA'TAA
Cuts at: 3010 3010
Size: 5440
PstI C_TGCA'G
Cuts at: 830 2792 830
Size: 1962 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5440
SacI G_AGCT'C
Cuts at: 2613 2613
Size: 5440
SalI G'TCGA_C
Cuts at: 1936 2782 1936
Size: 846 4594
SanDI GG'GwC_CC
Cuts at: 2379 2415 2379
Size: 36 5404
SbfI CC_TGCA'GG
Cuts at: 2792 2792
Size: 5440
ScaI AGT'ACT
Cuts at: 3688 5400 3688
Size: 1712 3728
SfcI C'TryA_G
Cuts at: 826 1045 2173 2788 3923 4604 4795 826
Size: 219 1128 615 1135 681 191 1471
Fragments arranged by size:
1471 1135 1128 681 615 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5440
SmaI CCC'GGG
Cuts at: 1872 2767 1872
Size: 895 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2084 3544 4445 4707 820
Size: 197 926 141 1460 901 262 1553
Fragments arranged by size:
1553 1460 926 901 262 197 141
SnaBI TAC'GTA
Cuts at: 493 3231 493
Size: 2738 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5440
SphI G_CATG'C
Cuts at: 1132 2798 1132
Size: 1666 3774
SspI AAT'ATT
Cuts at: 5 52 3364 5
Size: 47 3312 2081
Fragments arranged by size:
3312 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5440
StyI C'CwwG_G
Cuts at: 513 1065 2004 2074 2879 513
Size: 552 939 70 805 3074
Fragments arranged by size:
3074 939 805 552 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 3467 3484 3637 3822 4267 325
Size: 1255 417 1470 17 153 185 445 1498
Fragments arranged by size:
1498 1470 1255 445 417 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3686 5398 96
Size: 274 80 33 51 1443 1709 1712 138
Fragments arranged by size:
1712 1709 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2234 2266 5400 5432 2234
Size: 32 3134 32 2242
Fragments arranged by size:
3134 2242 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5440
XbaI T'CTAG_A
Cuts at: 2776 2776
Size: 5440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2236 2236
Size: 5440
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5440
XmaI C'CCGG_G
Cuts at: 1870 2765 1870
Size: 895 4545
XmnI GAAnn'nnTTC
Cuts at: 3569 3569
Size: 5440
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3248 276
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SapI SexAI SfiI SfoI SrfI SwaI