Restriction map for
pFN21AE3510
(Circular) (Six-base) MAPSORT of: pFN21AE3510.seq Check: 200 from: 1 to: 5914
pFN21AE3510 5914 bp
With 182 enzymes: SgfI *
March 28, 2012 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2313 3724 278
Size: 53 83 186 936 777 1411 2468
Fragments arranged by size:
2468 1411 936 777 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3257 1937
Size: 1320 4594
Acc65I G'GTAC_C
Cuts at: 3235 3235
Size: 5914
AclI AA'CG_TT
Cuts at: 1563 4041 4414 1563
Size: 2478 373 3063
Fragments arranged by size:
3063 2478 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2683 3078 3977 5361 5629 1632
Size: 1051 395 899 1384 268 1917
Fragments arranged by size:
1917 1384 1051 899 395 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5717
AflIII A'CryG_T
Cuts at: 1203 4813 5790 1203
Size: 3610 977 1327
Fragments arranged by size:
3610 1327 977
AgeI A'CCGG_T
Cuts at: 4745 4745
Size: 5914
AhdI GACnn_n'nnGTC
Cuts at: 4643 4643
Size: 5914
AleI CACnn'nnGTG
Cuts at: 1166 2141 1166
Size: 975 4939
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5914
AlwNI CAG_nnn'CTG
Cuts at: 2122 3657 5229 2122
Size: 1535 1572 2807
Fragments arranged by size:
2807 1572 1535
ApaI G_GGCC'C
Cuts at: 3187 3187
Size: 5914
ApaLI G'TGCA_C
Cuts at: 3971 5127 3971
Size: 1156 4758
ApoI r'AATT_y
Cuts at: 1493 3223 3449 3601 5710 1493
Size: 1730 226 152 2109 1697
Fragments arranged by size:
2109 1730 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4468 160
Size: 872 3436 1606
Fragments arranged by size:
3436 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
AvaI C'yCGr_G
Cuts at: 1870 1943 2928 3239 1870
Size: 73 985 311 4545
Fragments arranged by size:
4545 985 311 73
BamHI G'GATC_C
Cuts at: 2539 3244 3611 2539
Size: 705 367 4842
Fragments arranged by size:
4842 705 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3235 4690 618
Size: 325 217 627 1448 1455 1842
Fragments arranged by size:
1842 1455 1448 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2184 2370 2613 3187 3233 1391
Size: 793 186 243 574 46 4072
Fragments arranged by size:
4072 793 574 243 186 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4943
BbvCI CC'TCA_GC
Cuts at: 2418 2634 2657 2418
Size: 216 23 5675
Fragments arranged by size:
5675 216 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3020 3054 4105 4139 1903
Size: 34 1083 34 1051 34 3678
Fragments arranged by size:
3678 1083 1051 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3806 5016 3806
Size: 1210 4704
BclI T'GATC_A
Cuts at: 1548 2877 1548
Size: 1329 4585
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4525 136
Size: 107 122 71 631 605 2853 1525
Fragments arranged by size:
2853 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5909 5909
Size: 5914
BlpI GC'TnA_GC
Cuts at: 3331 3331
Size: 5914
Bme1580I G_kGCm'C
Cuts at: 1790 3175 3187 3975 5131 1790
Size: 1385 12 788 1156 2573
Fragments arranged by size:
2573 1385 1156 788 12
BmgBI CAC'GTC
Cuts at: 1318 2707 1318
Size: 1389 4525
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2059 3645 4603
Size: 416 53 83 399 24 642 1586 958
Cuts at: 4603 442
Size: 1753
Fragments arranged by size:
1753 1586 958 642 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5914
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2384 2384
Size: 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 4574 1239
Size: 1115 2220 2579
Fragments arranged by size:
2579 2220 1115
Bpu10I CC'TnA_GC
Cuts at: 2418 2634 2657 2418
Size: 216 23 5675
Fragments arranged by size:
5675 216 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4039 4904 5202 4039
Size: 865 298 4751
Fragments arranged by size:
4751 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2886 4577 882
Size: 617 1387 1691 2219
Fragments arranged by size:
2219 1691 1387 617
BsaAI yAC'GTr
Cuts at: 493 1204 3705 493
Size: 711 2501 2702
Fragments arranged by size:
2702 2501 711
BsaBI GATnn'nnATC
Cuts at: 2387 2585 5908 2387
Size: 198 3323 2393
Fragments arranged by size:
3323 2393 198
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2310 3721 4103 4738
Size: 53 83 186 936 777 1411 382 635
Cuts at: 4738 275
Size: 1451
Fragments arranged by size:
1451 1411 936 777 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2013 4347 4745 5019 5166 5577 2013
Size: 2334 398 274 147 411 2350
Fragments arranged by size:
2350 2334 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5689
Fragments arranged by size:
5689 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2387 1186
Size: 1027 174 4713
Fragments arranged by size:
4713 1027 174
BseYI C'CCAG_C
Cuts at: 3003 5117 5613 3003
Size: 2114 496 3304
Fragments arranged by size:
3304 2114 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2408 3135 1163
Size: 573 672 727 3942
Fragments arranged by size:
3942 727 672 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3364 4125 5153 1540
Size: 275 186 1363 761 1028 2301
Fragments arranged by size:
2301 1363 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2613 3975 4060 5131 2613
Size: 1362 85 1071 3396
Fragments arranged by size:
3396 1362 1071 85
BsmI GAATG_Cn'
Cuts at: 3425 3518 3425
Size: 93 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2370 2613 3175 3187 3233 3975
Size: 399 394 186 243 562 12 46 742
Cuts at: 3975 4060 5131 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 562 399 394 243 186
85 46 12
BspEI T'CCGG_A
Cuts at: 2013 5577 2013
Size: 3564 2350
BspHI T'CATG_A
Cuts at: 3803 5533 3803
Size: 1730 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3269 844
Size: 2425 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3146 3801 1394
Size: 429 1323 655 3507
Fragments arranged by size:
3507 1323 655 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2321 4409 4583 65
Size: 1183 1073 2088 174 1396
Fragments arranged by size:
2088 1396 1183 1073 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4558 4745 1700
Size: 110 2748 187 2869
Fragments arranged by size:
2869 2748 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5914
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5914
BssSI C'ACGA_G
Cuts at: 3974 4986 3974
Size: 1012 4902
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2710 794
Size: 1916 3998
BstBI TT'CG_AA
Cuts at: 4718 4718
Size: 5914
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5914
BstXI CCAn_nnnn'nTGG
Cuts at: 2143 2143
Size: 5914
BstYI r'GATC_y
Cuts at: 1972 2539 2847 3244 3611 3996 4013 5465 5551
Size: 567 308 397 367 385 17 1452 86
Cuts at: 5551 5563 5909 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 567 397 385 367 346 308
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2525 2853 2525
Size: 328 5586
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4423
Fragments arranged by size:
4423 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5175
Fragments arranged by size:
5175 683 56
BtsI GCAGTG_nn'
Cuts at: 3123 3430 4224 4244 3123
Size: 307 794 20 4793
Fragments arranged by size:
4793 794 307 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5914
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5879
DraI TTT'AAA
Cuts at: 3218 3218
Size: 5914
DraIII CAC_nnn'GTG
Cuts at: 2710 2710
Size: 5914
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2453 4921 809
Size: 507 1137 2468 1802
Fragments arranged by size:
2468 1802 1137 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 3361 4250
Size: 54 1425 50 275 18 214 1317 889
Cuts at: 4250 5879 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1317 889 275 214 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3361 1537
Size: 275 1549 4090
Fragments arranged by size:
4090 1549 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3844 1353
Size: 2491 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4501 4875 5021 387
Size: 1064 138 2912 374 146 1280
Fragments arranged by size:
2912 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2611 2611
Size: 5914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2354 2683 3078 3977 4574 5361 5629
Size: 393 722 329 395 899 597 787 268
Cuts at: 5629 1239
Size: 1524
Fragments arranged by size:
1524 899 787 722 597 395 393 329
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2256 2256
Size: 5914
EcoO109I rG'GnC_Cy
Cuts at: 3184 3184
Size: 5914
EcoRI G'AATT_C
Cuts at: 3223 3601 3223
Size: 378 5536
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 5914
FspI TGC'GCA
Cuts at: 2022 4420 2022
Size: 2398 3516
HaeII r_GCGC'y
Cuts at: 1678 5061 1678
Size: 3383 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2807 2839 2981 3013 4555
Size: 32 135 32 1235 32 142 32 1542
Cuts at: 4555 4587 4629 4661 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1542 1235 142 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3258 3504 669
Size: 988 281 1320 246 3079
Fragments arranged by size:
3079 1320 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5914
HpaI GTT'AAC
Cuts at: 3504 3504
Size: 5914
KpnI G_GTAC'C
Cuts at: 3239 3239
Size: 5914
MfeI C'AATT_G
Cuts at: 3513 3513
Size: 5914
MluI A'CGCG_T
Cuts at: 5790 5790
Size: 5914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2567 5028 5212 1305
Size: 1262 2461 184 2007
Fragments arranged by size:
2461 2007 1262 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4092
Fragments arranged by size:
4092 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 3872 4231 4390 518
Size: 648 975 1731 359 159 2042
Fragments arranged by size:
2042 1731 975 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2422 2661 2939 3034 3199 3205 3330 4007
Size: 816 239 278 95 165 6 125 677
Cuts at: 4007 5155 5400 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 816 677 278 245 239 165
125 95 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4423
Fragments arranged by size:
4423 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5914
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5914
NotI GC'GGCC_GC
Cuts at: 3361 3361
Size: 5914
NruI TCG'CGA
Cuts at: 2787 2787
Size: 5914
NspI r_CATG'y
Cuts at: 1132 1229 2347 2687 3272 4817 1132
Size: 97 1118 340 585 1545 2229
Fragments arranged by size:
2229 1545 1118 585 340 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5914
PciI A'CATG_T
Cuts at: 4813 4813
Size: 5914
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5914
PfoI T'CCnGG_A
Cuts at: 2370 2799 2370
Size: 429 5485
PmeI GTTT'AAAC
Cuts at: 3218 3218
Size: 5914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 5496 5528 3983
Size: 32 1481 32 4369
Fragments arranged by size:
4369 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5914
PsiI TTA'TAA
Cuts at: 3484 3484
Size: 5914
PspOMI G'GGCC_C
Cuts at: 3183 3183
Size: 5914
PstI C_TGCA'G
Cuts at: 830 3204 3266 830
Size: 2374 62 3478
Fragments arranged by size:
3478 2374 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5914
PvuII CAG'CTG
Cuts at: 2422 2661 3199 3205 2422
Size: 239 538 6 5131
Fragments arranged by size:
5131 538 239 6
SacI G_AGCT'C
Cuts at: 2613 2613
Size: 5914
SalI G'TCGA_C
Cuts at: 1936 3256 1936
Size: 1320 4594
SbfI CC_TGCA'GG
Cuts at: 3266 3266
Size: 5914
ScaI AGT'ACT
Cuts at: 4162 5874 4162
Size: 1712 4202
SexAI A'CCwGG_T
Cuts at: 2595 2672 2595
Size: 77 5837
SfcI C'TryA_G
Cuts at: 826 1045 3200 3262 4397 5078 5269 826
Size: 219 2155 62 1135 681 191 1471
Fragments arranged by size:
2155 1471 1135 681 219 191 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5914
SmaI CCC'GGG
Cuts at: 1872 3241 1872
Size: 1369 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4018 4919 5181 820
Size: 197 926 2075 901 262 1553
Fragments arranged by size:
2075 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3705 493
Size: 3212 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5914
SphI G_CATG'C
Cuts at: 1132 2347 3272 1132
Size: 1215 925 3774
Fragments arranged by size:
3774 1215 925
SspI AAT'ATT
Cuts at: 5 52 3838 5
Size: 47 3786 2081
Fragments arranged by size:
3786 2081 47
StuI AGG'CCT
Cuts at: 1517 3013 1517
Size: 1496 4418
StyI C'CwwG_G
Cuts at: 513 1065 2004 3089 3353 513
Size: 552 939 1085 264 3074
Fragments arranged by size:
3074 1085 939 552 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2757 2815 3941 3958 4111 4296 4741
Size: 1255 1177 58 1126 17 153 185 445
Cuts at: 4741 325
Size: 1498
Fragments arranged by size:
1498 1255 1177 1126 445 185 153 58
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4160 5872 96
Size: 274 80 33 51 1443 2183 1712 138
Fragments arranged by size:
2183 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2825 2857 5874 5906 2825
Size: 32 3017 32 2833
Fragments arranged by size:
3017 2833 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5914
XbaI T'CTAG_A
Cuts at: 3250 3250
Size: 5914
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5914
XmaI C'CCGG_G
Cuts at: 1870 3239 1870
Size: 1369 4545
XmnI GAAnn'nnTTC
Cuts at: 4043 4043
Size: 5914
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2311 3722 276
Size: 53 83 186 936 777 1411 2468
Fragments arranged by size:
2468 1411 936 777 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI