Restriction map for
pFN21AE3509
(Circular) (Six-base) MAPSORT of: pFN21AE3509.seq Check: 353 from: 1 to: 6445
pFN21AE3509 6445 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2913 3483 2913
Size: 570 5875
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4255 278
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3788 1937
Size: 1851 4594
Acc65I G'GTAC_C
Cuts at: 2645 3766 2645
Size: 1121 5324
AclI AA'CG_TT
Cuts at: 1563 3596 4572 4945 1563
Size: 2033 976 373 3063
Fragments arranged by size:
3063 2033 976 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3723 4508 5892 6160 1632
Size: 2091 785 1384 268 1917
Fragments arranged by size:
2091 1917 1384 785 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6248
AflIII A'CryG_T
Cuts at: 1203 5344 6321 1203
Size: 4141 977 1327
Fragments arranged by size:
4141 1327 977
AgeI A'CCGG_T
Cuts at: 5276 5276
Size: 6445
AhdI GACnn_n'nnGTC
Cuts at: 5174 5174
Size: 6445
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6445
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3314 1184
Size: 2130 4315
AlwNI CAG_nnn'CTG
Cuts at: 3737 4188 5760 3737
Size: 451 1572 4422
Fragments arranged by size:
4422 1572 451
ApaI G_GGCC'C
Cuts at: 3729 3729
Size: 6445
ApaLI G'TGCA_C
Cuts at: 4502 5658 4502
Size: 1156 5289
ApoI r'AATT_y
Cuts at: 1493 3125 3754 3980 4132 6241 1493
Size: 1632 629 226 152 2109 1697
Fragments arranged by size:
2109 1697 1632 629 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4999 160
Size: 872 3967 1606
Fragments arranged by size:
3967 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
AvaI C'yCGr_G
Cuts at: 1870 1943 2622 3539 3770 1870
Size: 73 679 917 231 4545
Fragments arranged by size:
4545 917 679 231 73
BamHI G'GATC_C
Cuts at: 3775 4142 3775
Size: 367 6078
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2645 3766 5221 618
Size: 325 217 627 858 1121 1455 1842
Fragments arranged by size:
1842 1455 1121 858 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2782 3281 3510 3729 3764 1391
Size: 1391 499 229 219 35 4072
Fragments arranged by size:
4072 1391 499 229 219 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2434 2701 928
Size: 971 535 267 4672
Fragments arranged by size:
4672 971 535 267
BbvCI CC'TCA_GC
Cuts at: 3572 3572
Size: 6445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4636 4670 1903
Size: 34 2699 34 3678
Fragments arranged by size:
3678 2699 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4337 5547 4337
Size: 1210 5235
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6445
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3336 5056 136
Size: 107 122 71 631 605 1664 1720 1525
Fragments arranged by size:
1720 1664 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2808 6440 2808
Size: 3632 2813
BlpI GC'TnA_GC
Cuts at: 3269 3862 3269
Size: 593 5852
Bme1580I G_kGCm'C
Cuts at: 1790 3403 3729 4506 5662 1790
Size: 1613 326 777 1156 2573
Fragments arranged by size:
2573 1613 1156 777 326
BmgBI CAC'GTC
Cuts at: 1318 2440 1318
Size: 1122 5323
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2162 4176 5134
Size: 416 53 83 399 24 745 2014 958
Cuts at: 5134 442
Size: 1753
Fragments arranged by size:
2014 1753 958 745 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2402 1056
Size: 1346 5099
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2310 5105 1239
Size: 1071 2795 2579
Fragments arranged by size:
2795 2579 1071
Bpu10I CC'TnA_GC
Cuts at: 2030 3504 3572 2030
Size: 1474 68 4903
Fragments arranged by size:
4903 1474 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4570 5435 5733 4570
Size: 865 298 5282
Fragments arranged by size:
5282 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2169 2497 2762 2825 3734 5108 882
Size: 617 670 328 265 63 909 1374 2219
Fragments arranged by size:
2219 1374 909 670 617 328 265 63
BsaAI yAC'GTr
Cuts at: 493 1204 2458 4236 493
Size: 711 1254 1778 2702
Fragments arranged by size:
2702 1778 1254 711
BsaBI GATnn'nnATC
Cuts at: 2117 6439 2117
Size: 4322 2123
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4252 4634 5269 275
Size: 53 83 186 936 2719 382 635 1451
Fragments arranged by size:
2719 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4878 5276 5550 5697 6108 4878
Size: 398 274 147 411 5215
Fragments arranged by size:
5215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6220
Fragments arranged by size:
6220 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2050 2780 3578 1186
Size: 864 730 798 4053
Fragments arranged by size:
4053 864 798 730
BseYI C'CCAG_C
Cuts at: 2772 2835 3301 3320 3509 3730 5648 6144 2772
Size: 63 466 19 189 221 1918 496 3073
Fragments arranged by size:
3073 1918 496 466 221 189 63 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5872
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3895 4656 5684 1540
Size: 275 186 1894 761 1028 2301
Fragments arranged by size:
2301 1894 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3094 3281 3529 4506 4591 5662 3094
Size: 187 248 977 85 1071 3877
Fragments arranged by size:
3877 1071 977 248 187 85
BsmI GAATG_Cn'
Cuts at: 3956 4049 3956
Size: 93 6352
BsmBI CGTCTCn'nnnn_
Cuts at: 2236 2236
Size: 6445
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2782 3094 3281 3403 3510 3529 3729
Size: 399 992 312 187 122 107 19 200
Cuts at: 3729 3764 4506 4591 5662 1391
Size: 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 992 742 399 312 200 187
122 107 85 35 19
BspEI T'CCGG_A
Cuts at: 6108 6108
Size: 6445
BspHI T'CATG_A
Cuts at: 4334 6064 4334
Size: 1730 4715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2207 2904 2913 3228 3483 3800 844
Size: 1363 697 9 315 255 317 3489
Fragments arranged by size:
3489 1363 697 317 315 255 9
BsrBI CCG'CTC
Cuts at: 1394 1823 4332 1394
Size: 429 2509 3507
Fragments arranged by size:
3507 2509 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4940 5114 65
Size: 1183 3692 174 1396
Fragments arranged by size:
3692 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5089 5276 1700
Size: 110 3279 187 2869
Fragments arranged by size:
3279 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6445
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6445
BssSI C'ACGA_G
Cuts at: 4505 5517 4505
Size: 1012 5433
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2752 794
Size: 1958 4487
BstBI TT'CG_AA
Cuts at: 5249 5249
Size: 6445
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6445
BstXI CCAn_nnnn'nTGG
Cuts at: 2354 3490 2354
Size: 1136 5309
BstYI r'GATC_y
Cuts at: 1972 2808 3078 3579 3775 4142 4527 4544 5996
Size: 836 270 501 196 367 385 17 1452
Cuts at: 5996 6082 6094 6440 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 836 501 385 367 346 270
196 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2876 2876
Size: 6445
BtgI C'CryG_G
Cuts at: 513 1065 2004 2404 513
Size: 552 939 400 4554
Fragments arranged by size:
4554 939 552 400
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2238 487
Size: 683 56 1012 4694
Fragments arranged by size:
4694 1012 683 56
BtsI GCAGTG_nn'
Cuts at: 2519 3065 3961 4755 4775 2519
Size: 546 896 794 20 4189
Fragments arranged by size:
4189 896 794 546 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6410
DraI TTT'AAA
Cuts at: 3749 3749
Size: 6445
DraIII CAC_nnn'GTG
Cuts at: 2218 3240 2218
Size: 1022 5423
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2250 5452 809
Size: 507 934 3202 1802
Fragments arranged by size:
3202 1802 934 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2191 2503 2616
Size: 54 1425 50 275 18 361 312 113
Cuts at: 2616 3130 3651 3892 4781 6410 8
Size: 514 521 241 889 1629 43
Fragments arranged by size:
1629 1425 889 521 514 361 312 275
241 113 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3892 1537
Size: 275 2080 4090
Fragments arranged by size:
4090 2080 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 2082 2087 2300 2474 4375 1353
Size: 689 40 5 213 174 1901 3423
Fragments arranged by size:
3423 1901 689 213 174 40 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5032 5406 5552 387
Size: 1064 138 3443 374 146 1280
Fragments arranged by size:
3443 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3279 3279
Size: 6445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 3723 4508 5105 5892 6160 1239
Size: 393 678 1413 785 597 787 268 1524
Fragments arranged by size:
1524 1413 787 785 678 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2795 2879 2795
Size: 84 6361
EcoO109I rG'GnC_Cy
Cuts at: 2335 2463 3015 3382 3535 3679 3725 3726 2335
Size: 128 552 367 153 144 46 1 5054
Fragments arranged by size:
5054 552 367 153 144 128 46 1
EcoRI G'AATT_C
Cuts at: 3754 4132 3754
Size: 378 6067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2076 3685 2076
Size: 1609 4836
FspI TGC'GCA
Cuts at: 4951 4951
Size: 6445
HaeII r_GCGC'y
Cuts at: 1678 5592 1678
Size: 3914 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 5086 5118 5160
Size: 32 135 32 980 32 2502 32 42
Cuts at: 5160 5192 1373
Size: 32 2626
Fragments arranged by size:
2626 2502 980 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3789 4035 669
Size: 988 281 1851 246 3079
Fragments arranged by size:
3079 1851 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6445
HpaI GTT'AAC
Cuts at: 4035 4035
Size: 6445
KpnI G_GTAC'C
Cuts at: 2649 3770 2649
Size: 1121 5324
MfeI C'AATT_G
Cuts at: 4044 4044
Size: 6445
MluI A'CGCG_T
Cuts at: 6321 6321
Size: 6445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2952 5559 5743 1305
Size: 1647 2607 184 2007
Fragments arranged by size:
2607 2007 1647 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2618 10
Size: 54 1425 343 786 3837
Fragments arranged by size:
3837 1425 786 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4403 4762 4921 518
Size: 648 3237 359 159 2042
Fragments arranged by size:
3237 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2704 2776 2869 3226 3861 4538 5686 5931
Size: 1098 72 93 357 635 677 1148 245
Cuts at: 5931 1606
Size: 2120
Fragments arranged by size:
2120 1148 1098 677 635 357 245 93
72
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4954
Fragments arranged by size:
4954 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6445
NheI G'CTAG_C
Cuts at: 1052 2398 1052
Size: 1346 5099
NotI GC'GGCC_GC
Cuts at: 3892 3892
Size: 6445
NruI TCG'CGA
Cuts at: 3003 3003
Size: 6445
NspI r_CATG'y
Cuts at: 1132 1229 3691 3803 5348 1132
Size: 97 2462 112 1545 2229
Fragments arranged by size:
2462 2229 1545 112 97
PasI CC'CwG_GG
Cuts at: 1348 2990 1348
Size: 1642 4803
PciI A'CATG_T
Cuts at: 5344 5344
Size: 6445
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3262 1108
Size: 2154 4291
PmeI GTTT'AAAC
Cuts at: 3749 3749
Size: 6445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4514 4546 6027 6059 4514
Size: 32 1481 32 4900
Fragments arranged by size:
4900 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2463 3015 3382 3535 2463
Size: 552 367 153 5373
Fragments arranged by size:
5373 552 367 153
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6445
PsiI TTA'TAA
Cuts at: 4015 4015
Size: 6445
PspOMI G'GGCC_C
Cuts at: 3725 3725
Size: 6445
PstI C_TGCA'G
Cuts at: 830 2691 3225 3696 3797 830
Size: 1861 534 471 101 3478
Fragments arranged by size:
3478 1861 534 471 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6445
PvuII CAG'CTG
Cuts at: 2869 2869
Size: 6445
SacI G_AGCT'C
Cuts at: 3281 3281
Size: 6445
SalI G'TCGA_C
Cuts at: 1936 3787 1936
Size: 1851 4594
SanDI GG'GwC_CC
Cuts at: 2463 3015 2463
Size: 552 5893
SbfI CC_TGCA'GG
Cuts at: 3797 3797
Size: 6445
ScaI AGT'ACT
Cuts at: 4693 6405 4693
Size: 1712 4733
SfcI C'TryA_G
Cuts at: 826 1045 2687 3221 3692 3793 4928 5609 5800
Size: 219 1642 534 471 101 1135 681 191
Cuts at: 5800 826
Size: 1471
Fragments arranged by size:
1642 1471 1135 681 534 471 219 191
101
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6445
SmaI CCC'GGG
Cuts at: 1872 3541 3772 1872
Size: 1669 231 4545
Fragments arranged by size:
4545 1669 231
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4549 5450 5712 820
Size: 197 926 2606 901 262 1553
Fragments arranged by size:
2606 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4236 493
Size: 3743 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6445
SphI G_CATG'C
Cuts at: 1132 3803 1132
Size: 2671 3774
SspI AAT'ATT
Cuts at: 5 52 4369 5
Size: 47 4317 2081
Fragments arranged by size:
4317 2081 47
StuI AGG'CCT
Cuts at: 1517 3557 1517
Size: 2040 4405
StyI C'CwwG_G
Cuts at: 513 1065 2004 2430 2556 2576 3109 3345 3884
Size: 552 939 426 126 20 533 236 539
Cuts at: 3884 513
Size: 3074
Fragments arranged by size:
3074 939 552 539 533 426 236 126
20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3207 4472 4489 4642 4827 5272 325
Size: 1255 1627 1265 17 153 185 445 1498
Fragments arranged by size:
1627 1498 1265 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4691 6403 96
Size: 274 80 33 51 1443 2714 1712 138
Fragments arranged by size:
2714 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2222 2254 2564 2596 6405 6437 2222
Size: 32 310 32 3809 32 2230
Fragments arranged by size:
3809 2230 310 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2262 1316
Size: 946 5499
XbaI T'CTAG_A
Cuts at: 3781 3781
Size: 6445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3490 3490
Size: 6445
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6445
XmaI C'CCGG_G
Cuts at: 1870 3539 3770 1870
Size: 1669 231 4545
Fragments arranged by size:
4545 1669 231
XmnI GAAnn'nnTTC
Cuts at: 3665 4574 3665
Size: 909 5536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4253 276
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacII SapI SexAI SfiI SfoI SrfI SwaI