(Circular) (Six-base) MAPSORT of: pFN21AE3508.seq Check: 2106 from: 1 to: 6814 pFN21AE3508 6814 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4624 278 Size: 53 83 186 936 3088 2468 Fragments arranged by size: 3088 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4157 1937 Size: 2220 4594 Acc65I G'GTAC_C Cuts at: 2193 4135 2193 Size: 1942 4872 AclI AA'CG_TT Cuts at: 1563 4941 5314 1563 Size: 3378 373 3063 Fragments arranged by size: 3378 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3156 3945 4877 6261 6529 1632 Size: 1524 789 932 1384 268 1917 Fragments arranged by size: 1917 1524 1384 932 789 268 AfeI AGC'GCT Cuts at: 3522 3522 Size: 6814 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6617 AflIII A'CryG_T Cuts at: 1203 3576 5713 6690 1203 Size: 2373 2137 977 1327 Fragments arranged by size: 2373 2137 1327 977 AgeI A'CCGG_T Cuts at: 5645 5645 Size: 6814 AhdI GACnn_n'nnGTC Cuts at: 5543 5543 Size: 6814 AleI CACnn'nnGTG Cuts at: 1166 3320 1166 Size: 2154 4660 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2484 3416 1184 Size: 1300 932 4582 Fragments arranged by size: 4582 1300 932 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4080 4112 4080 Size: 32 6782 AlwNI CAG_nnn'CTG Cuts at: 2069 2383 3065 4557 6129 2069 Size: 314 682 1492 1572 2754 Fragments arranged by size: 2754 1572 1492 682 314 ApaI G_GGCC'C Cuts at: 2933 3133 3821 2933 Size: 200 688 5926 Fragments arranged by size: 5926 688 200 ApaLI G'TGCA_C Cuts at: 4871 6027 4871 Size: 1156 5658 ApoI r'AATT_y Cuts at: 1493 4123 4349 4501 6610 1493 Size: 2630 226 152 2109 1697 Fragments arranged by size: 2630 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5368 160 Size: 872 4336 1606 Fragments arranged by size: 4336 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6814 AvaI C'yCGr_G Cuts at: 1870 1943 2043 2211 2516 2691 4139 1870 Size: 73 100 168 305 175 1448 4545 Fragments arranged by size: 4545 1448 305 175 168 100 73 BamHI G'GATC_C Cuts at: 4144 4511 4144 Size: 367 6447 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2025 2193 2468 3602 3674 Size: 325 217 627 238 168 275 1134 72 Cuts at: 3674 4016 4135 5590 618 Size: 342 119 1455 1842 Fragments arranged by size: 1842 1455 1134 627 342 325 275 238 217 168 119 72 BanII G_rGCy'C Cuts at: 1391 2157 2631 2774 2880 2933 3133 3821 3828 Size: 766 474 143 106 53 200 688 7 Cuts at: 3828 3963 4133 1391 Size: 135 170 4072 Fragments arranged by size: 4072 766 688 474 200 170 143 135 106 53 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5843 BbvCI CC'TCA_GC Cuts at: 2717 2717 Size: 6814 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5005 5039 1903 Size: 34 3068 34 3678 Fragments arranged by size: 3678 3068 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3336 4706 5916 3336 Size: 1370 1210 4234 Fragments arranged by size: 4234 1370 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6814 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2577 2586 5425 Size: 107 122 71 631 605 905 9 2839 Cuts at: 5425 136 Size: 1525 Fragments arranged by size: 2839 1525 905 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 6809 6809 Size: 6814 BlpI GC'TnA_GC Cuts at: 3746 4231 3746 Size: 485 6329 Bme1580I G_kGCm'C Cuts at: 1790 2030 2415 2716 2902 2933 3133 3607 3821 Size: 240 385 301 186 31 200 474 214 Cuts at: 3821 4875 6031 1790 Size: 1054 1156 2573 Fragments arranged by size: 2573 1156 1054 474 385 301 240 214 200 186 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6814 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3154 3356 4545 Size: 416 53 83 399 24 1737 202 1189 Cuts at: 4545 5503 442 Size: 958 1753 Fragments arranged by size: 1753 1737 1189 958 416 399 202 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2562 1056 Size: 1506 5308 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3817 3817 Size: 6814 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2076 3011 3058 3263 3653 3812 3893 3986 Size: 837 935 47 205 390 159 81 93 Cuts at: 3986 4087 5474 1239 Size: 101 1387 2579 Fragments arranged by size: 2579 1387 935 837 390 205 159 101 93 81 47 Bpu10I CC'TnA_GC Cuts at: 2717 3822 2717 Size: 1105 5709 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3683 3695 4939 5804 6102 3683 Size: 12 1244 865 298 4395 Fragments arranged by size: 4395 1244 865 298 12 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2219 2224 3062 5477 882 Size: 617 720 5 838 2415 2219 Fragments arranged by size: 2415 2219 838 720 617 5 BsaAI yAC'GTr Cuts at: 493 1204 2298 2410 4605 493 Size: 711 1094 112 2195 2702 Fragments arranged by size: 2702 2195 1094 711 112 BsaBI GATnn'nnATC Cuts at: 6808 6808 Size: 6814 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4621 5003 5638 275 Size: 53 83 186 936 3088 382 635 1451 Fragments arranged by size: 3088 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5247 5645 5919 6066 6477 5247 Size: 398 274 147 411 5584 Fragments arranged by size: 5584 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6589 Fragments arranged by size: 6589 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 2165 3524 1186 Size: 976 3 1359 4476 Fragments arranged by size: 4476 1359 976 3 BseYI C'CCAG_C Cuts at: 2035 2880 3387 6017 6513 2035 Size: 845 507 2630 496 2336 Fragments arranged by size: 2630 2336 845 507 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2726 3478 3512 1163 Size: 573 990 752 34 4465 Fragments arranged by size: 4465 990 752 573 34 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3552 3956 4264 5025 6053 1540 Size: 275 186 1551 404 308 761 1028 2301 Fragments arranged by size: 2301 1551 1028 761 404 308 275 186 BsiHKAI G_wGCw'C Cuts at: 2157 2295 3846 3963 4875 4960 6031 2157 Size: 138 1551 117 912 85 1071 2940 Fragments arranged by size: 2940 1551 1071 912 138 117 85 BsmI GAATG_Cn' Cuts at: 4325 4418 4325 Size: 93 6721 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2157 2295 2415 2631 2716 2774 Size: 399 240 127 138 120 216 85 58 Cuts at: 2774 2880 2902 2933 3133 3607 3821 3828 3846 Size: 106 22 31 200 474 214 7 18 Cuts at: 3846 3963 4133 4875 4960 6031 1391 Size: 117 170 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 474 399 240 216 214 200 170 138 127 120 117 106 85 85 58 31 22 18 7 BspEI T'CCGG_A Cuts at: 6477 6477 Size: 6814 BspHI T'CATG_A Cuts at: 4703 6433 4703 Size: 1730 5084 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2748 3447 4099 4169 844 Size: 1904 699 652 70 3489 Fragments arranged by size: 3489 1904 699 652 70 BsrBI CCG'CTC Cuts at: 1394 1823 3514 4101 4701 1394 Size: 429 1691 587 600 3507 Fragments arranged by size: 3507 1691 600 587 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2840 5309 5483 65 Size: 1183 1592 2469 174 1396 Fragments arranged by size: 2469 1592 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3234 3420 3497 5458 5645 1700 Size: 110 1424 186 77 1961 187 2869 Fragments arranged by size: 2869 1961 1424 187 186 110 77 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6814 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6814 BssSI C'ACGA_G Cuts at: 2420 4874 5886 2420 Size: 2454 1012 3348 Fragments arranged by size: 3348 2454 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2846 794 Size: 2052 4762 BstBI TT'CG_AA Cuts at: 5618 5618 Size: 6814 BstEII G'GTnAC_C Cuts at: 1184 2644 3646 1184 Size: 1460 1002 4352 Fragments arranged by size: 4352 1460 1002 BstXI CCAn_nnnn'nTGG Cuts at: 2957 3472 3544 4111 2957 Size: 515 72 567 5660 Fragments arranged by size: 5660 567 515 72 BstYI r'GATC_y Cuts at: 1972 2173 2730 4144 4511 4896 4913 6365 6451 Size: 201 557 1414 367 385 17 1452 86 Cuts at: 6451 6463 6809 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1414 557 385 367 346 201 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2575 2575 Size: 6814 BtgI C'CryG_G Cuts at: 513 1065 2197 2911 3415 3477 3898 513 Size: 552 1132 714 504 62 421 3429 Fragments arranged by size: 3429 1132 714 552 504 421 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2103 2670 487 Size: 683 56 877 567 4631 Fragments arranged by size: 4631 877 683 567 56 BtsI GCAGTG_nn' Cuts at: 2504 2631 4065 4330 5124 5144 2504 Size: 127 1434 265 794 20 4174 Fragments arranged by size: 4174 1434 794 265 127 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6814 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6779 DraI TTT'AAA Cuts at: 4118 4118 Size: 6814 DraIII CAC_nnn'GTG Cuts at: 3850 3850 Size: 6814 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5821 809 Size: 507 4505 1802 Fragments arranged by size: 4505 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2200 3010 3198 Size: 54 1425 50 275 18 370 810 188 Cuts at: 3198 3236 3418 3474 3549 3953 4261 5150 6779 Size: 38 182 56 75 404 308 889 1629 Cuts at: 6779 8 Size: 43 Fragments arranged by size: 1629 1425 889 810 404 370 308 275 188 182 75 56 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 3549 3953 4261 1537 Size: 275 1737 404 308 4090 Fragments arranged by size: 4090 1737 404 308 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4744 1353 Size: 3391 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5401 5775 5921 387 Size: 1064 138 3812 374 146 1280 Fragments arranged by size: 3812 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2155 3961 2155 Size: 1806 5008 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2076 3011 3058 3156 3263 3653 3812 Size: 393 444 935 47 98 107 390 159 Cuts at: 3812 3893 3945 3986 4087 4877 5474 6261 6529 Size: 81 52 41 101 790 597 787 268 Cuts at: 6529 1239 Size: 1524 Fragments arranged by size: 1524 935 790 787 597 444 393 390 268 159 107 101 98 81 52 47 41 EcoNI CCTnn'n_nnAGG Cuts at: 2537 2960 3722 2537 Size: 423 762 5629 Fragments arranged by size: 5629 762 423 EcoO109I rG'GnC_Cy Cuts at: 2040 2579 2929 2930 3129 3130 3817 3818 4060 Size: 539 350 1 199 1 687 1 242 Cuts at: 4060 2040 Size: 4794 Fragments arranged by size: 4794 687 539 350 242 199 1 1 1 EcoRI G'AATT_C Cuts at: 4123 4501 4123 Size: 378 6436 FspI TGC'GCA Cuts at: 3260 5320 3260 Size: 2060 4754 HaeII r_GCGC'y Cuts at: 1678 3524 4042 5961 1678 Size: 1846 518 1919 2531 Fragments arranged by size: 2531 1919 1846 518 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2729 2761 2990 3022 5455 Size: 32 135 32 1157 32 229 32 2433 Cuts at: 5455 5487 5529 5561 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2433 1157 229 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4158 4404 669 Size: 988 281 2220 246 3079 Fragments arranged by size: 3079 2220 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6814 HpaI GTT'AAC Cuts at: 4404 4404 Size: 6814 KpnI G_GTAC'C Cuts at: 2197 4139 2197 Size: 1942 4872 MfeI C'AATT_G Cuts at: 4413 4413 Size: 6814 MluI A'CGCG_T Cuts at: 6690 6690 Size: 6814 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5928 6112 1305 Size: 4623 184 2007 Fragments arranged by size: 4623 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3012 10 Size: 54 1425 343 1180 3812 Fragments arranged by size: 3812 1425 1180 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3320 3918 4772 5131 5290 518 Size: 648 2154 598 854 359 159 2042 Fragments arranged by size: 2154 2042 854 648 598 359 159 MspA1I CmG'CkG Cuts at: 1606 2304 2709 2884 3387 3544 3745 4230 4907 Size: 698 405 175 503 157 201 485 677 Cuts at: 4907 6055 6300 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 698 677 503 485 405 245 201 175 157 NaeI GCC'GGC Cuts at: 3422 3422 Size: 6814 NcoI C'CATG_G Cuts at: 513 1065 2197 2911 3415 513 Size: 552 1132 714 504 3912 Fragments arranged by size: 3912 1132 714 552 504 NdeI CA'TA_TG Cuts at: 387 387 Size: 6814 NgoMIV G'CCGG_C Cuts at: 3420 3420 Size: 6814 NheI G'CTAG_C Cuts at: 1052 2558 1052 Size: 1506 5308 NotI GC'GGCC_GC Cuts at: 4261 4261 Size: 6814 NspI r_CATG'y Cuts at: 1132 1229 3314 3580 4172 5717 1132 Size: 97 2085 266 592 1545 2229 Fragments arranged by size: 2229 2085 1545 592 266 97 PasI CC'CwG_GG Cuts at: 1348 3225 3828 1348 Size: 1877 603 4334 Fragments arranged by size: 4334 1877 603 PciI A'CATG_T Cuts at: 3576 5713 3576 Size: 2137 4677 PflMI CCAn_nnn'nTGG Cuts at: 1108 2069 4084 1108 Size: 961 2015 3838 Fragments arranged by size: 3838 2015 961 PfoI T'CCnGG_A Cuts at: 2109 2259 2890 3963 2109 Size: 150 631 1073 4960 Fragments arranged by size: 4960 1073 631 150 PmeI GTTT'AAAC Cuts at: 4118 4118 Size: 6814 PmlI CAC'GTG Cuts at: 2298 2410 2298 Size: 112 6702 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4883 4915 6396 6428 4883 Size: 32 1481 32 5269 Fragments arranged by size: 5269 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2040 4060 2040 Size: 2020 4794 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6814 PsiI TTA'TAA Cuts at: 4384 4384 Size: 6814 PspOMI G'GGCC_C Cuts at: 2929 3129 3817 2929 Size: 200 688 5926 Fragments arranged by size: 5926 688 200 PspXI vC'TCGA_Gb Cuts at: 2516 2516 Size: 6814 PstI C_TGCA'G Cuts at: 830 2824 3939 4166 830 Size: 1994 1115 227 3478 Fragments arranged by size: 3478 1994 1115 227 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6814 PvuII CAG'CTG Cuts at: 2709 2884 3544 2709 Size: 175 660 5979 Fragments arranged by size: 5979 660 175 SacI G_AGCT'C Cuts at: 2157 3963 2157 Size: 1806 5008 SalI G'TCGA_C Cuts at: 1936 4156 1936 Size: 2220 4594 SanDI GG'GwC_CC Cuts at: 2040 2040 Size: 6814 SbfI CC_TGCA'GG Cuts at: 4166 4166 Size: 6814 ScaI AGT'ACT Cuts at: 5062 6774 5062 Size: 1712 5102 SfcI C'TryA_G Cuts at: 826 1045 2788 2820 2992 3935 4162 5297 5978 Size: 219 1743 32 172 943 227 1135 681 Cuts at: 5978 6169 826 Size: 191 1471 Fragments arranged by size: 1743 1471 1135 943 681 227 219 191 172 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6814 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6814 SmaI CCC'GGG Cuts at: 1872 4141 1872 Size: 2269 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2516 3698 3710 4918 5819 6081 Size: 197 926 573 1182 12 1208 901 262 Cuts at: 6081 820 Size: 1553 Fragments arranged by size: 1553 1208 1182 926 901 573 262 197 12 SnaBI TAC'GTA Cuts at: 493 4605 493 Size: 4112 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6814 SphI G_CATG'C Cuts at: 1132 4172 1132 Size: 3040 3774 SspI AAT'ATT Cuts at: 5 52 4738 5 Size: 47 4686 2081 Fragments arranged by size: 4686 2081 47 StuI AGG'CCT Cuts at: 1517 2318 2966 3906 1517 Size: 801 648 940 4425 Fragments arranged by size: 4425 940 801 648 StyI C'CwwG_G Cuts at: 513 1065 2094 2197 2229 2583 2911 2956 3318 Size: 552 1029 103 32 354 328 45 362 Cuts at: 3318 3357 3415 4253 513 Size: 39 58 838 3074 Fragments arranged by size: 3074 1029 838 552 362 354 328 103 58 45 39 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2636 2764 3590 3634 4841 4858 5011 Size: 1255 1056 128 826 44 1207 17 153 Cuts at: 5011 5196 5641 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1207 1056 826 445 185 153 128 44 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2103 5060 6772 Size: 274 80 33 51 1443 126 2957 1712 Cuts at: 6772 96 Size: 138 Fragments arranged by size: 2957 1712 1443 274 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3204 3236 3446 3478 3745 3777 6774 6806 3204 Size: 32 210 32 267 32 2997 32 3212 Fragments arranged by size: 3212 2997 267 210 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2642 4058 1316 Size: 1326 1416 4072 Fragments arranged by size: 4072 1416 1326 XbaI T'CTAG_A Cuts at: 4150 4150 Size: 6814 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2530 2011 Size: 519 6295 XhoI C'TCGA_G Cuts at: 1943 2516 1943 Size: 573 6241 XmaI C'CCGG_G Cuts at: 1870 4139 1870 Size: 2269 4545 XmnI GAAnn'nnTTC Cuts at: 4943 4943 Size: 6814 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4622 276 Size: 53 83 186 936 3088 2468 Fragments arranged by size: 3088 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI