Restriction map for
pFN21AE3507
(Circular) (Six-base) MAPSORT of: pFN21AE3507.seq Check: 5782 from: 1 to: 7492
pFN21AE3507 7492 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3715 3790 3715
Size: 75 7417
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3710 5302 278
Size: 53 83 186 936 2174 1592 2468
Fragments arranged by size:
2468 2174 1592 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4835 1937
Size: 2898 4594
Acc65I G'GTAC_C
Cuts at: 4813 4813
Size: 7492
AclI AA'CG_TT
Cuts at: 1563 5619 5992 1563
Size: 4056 373 3063
Fragments arranged by size:
4056 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3134 3343 5555 6939 7207 1632
Size: 1502 209 2212 1384 268 1917
Fragments arranged by size:
2212 1917 1502 1384 268 209
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7295
AflIII A'CryG_T
Cuts at: 1203 2316 6391 7368 1203
Size: 1113 4075 977 1327
Fragments arranged by size:
4075 1327 1113 977
AgeI A'CCGG_T
Cuts at: 3327 6323 3327
Size: 2996 4496
AhdI GACnn_n'nnGTC
Cuts at: 2050 3756 6221 2050
Size: 1706 2465 3321
Fragments arranged by size:
3321 2465 1706
AleI CACnn'nnGTG
Cuts at: 1166 3869 4034 1166
Size: 2703 165 4624
Fragments arranged by size:
4624 2703 165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3866 4377 4502 1184
Size: 2682 511 125 4174
Fragments arranged by size:
4174 2682 511 125
AlwNI CAG_nnn'CTG
Cuts at: 2197 3299 3565 3606 4138 4432 5235 6807 2197
Size: 1102 266 41 532 294 803 1572 2882
Fragments arranged by size:
2882 1572 1102 803 532 294 266 41
ApaI G_GGCC'C
Cuts at: 2227 3006 2227
Size: 779 6713
ApaLI G'TGCA_C
Cuts at: 2417 3035 5549 6705 2417
Size: 618 2514 1156 3204
Fragments arranged by size:
3204 2514 1156 618
ApoI r'AATT_y
Cuts at: 1493 2452 3425 4801 5027 5179 7288 1493
Size: 959 973 1376 226 152 2109 1697
Fragments arranged by size:
2109 1697 1376 973 959 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6046 160
Size: 872 5014 1606
Fragments arranged by size:
5014 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7492
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 2096 2140 3287 3405 3683 4817
Size: 73 84 69 44 1147 118 278 1134
Cuts at: 4817 1870
Size: 4545
Fragments arranged by size:
4545 1147 1134 278 118 84 73 69
44
BamHI G'GATC_C
Cuts at: 2739 3048 3084 4822 5189 2739
Size: 309 36 1738 367 5042
Fragments arranged by size:
5042 1738 367 309 36
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2110 2520 3317 3614 3925
Size: 325 217 627 323 410 797 297 311
Cuts at: 3925 4813 6268 618
Size: 888 1455 1842
Fragments arranged by size:
1842 1455 888 797 627 410 325 323
311 297 217
BanII G_rGCy'C
Cuts at: 1391 2227 3006 3282 3660 3816 4065 4101 4204
Size: 836 779 276 378 156 249 36 103
Cuts at: 4204 4811 1391
Size: 607 4072
Fragments arranged by size:
4072 836 779 607 378 276 249 156
103 36
BbeI G_GCGC'C
Cuts at: 2114 3321 2114
Size: 1207 6285
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2974 3337 4670 928
Size: 971 1075 363 1333 3750
Fragments arranged by size:
3750 1333 1075 971 363
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2376 2410 3826 3860 5683 5717 1903
Size: 34 439 34 1416 34 1823 34 3678
Fragments arranged by size:
3678 1823 1416 439 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5384 6594 5384
Size: 1210 6282
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7492
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2550 3664 6103
Size: 107 122 71 631 605 878 1114 2439
Cuts at: 6103 136
Size: 1525
Fragments arranged by size:
2439 1525 1114 878 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7487 7487
Size: 7492
BlpI GC'TnA_GC
Cuts at: 3276 4909 3276
Size: 1633 5859
Bme1580I G_kGCm'C
Cuts at: 1790 2163 2227 2421 3006 3039 3617 4031 4075
Size: 373 64 194 585 33 578 414 44
Cuts at: 4075 5553 6709 1790
Size: 1478 1156 2573
Fragments arranged by size:
2573 1478 1156 585 578 414 373 194
64 44 33
BmgBI CAC'GTC
Cuts at: 1318 3382 1318
Size: 2064 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2536 3190 5223
Size: 416 53 83 399 24 1119 654 2033
Cuts at: 5223 6181 442
Size: 958 1753
Fragments arranged by size:
2033 1753 1119 958 654 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7492
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 7492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2290 2462 2473 2866 3038 3410 4073
Size: 935 116 172 11 393 172 372 663
Cuts at: 4073 4131 4409 4757 6152 1239
Size: 58 278 348 1395 2579
Fragments arranged by size:
2579 1395 935 663 393 372 348 278
172 172 116 58 11
Bpu10I CC'TnA_GC
Cuts at: 2990 4263 4710 2990
Size: 1273 447 5772
Fragments arranged by size:
5772 1273 447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3989 4359 5617 6482 6780 3989
Size: 370 1258 865 298 4701
Fragments arranged by size:
4701 1258 865 370 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2303 2842 4577 6155 882
Size: 617 804 539 1735 1578 2219
Fragments arranged by size:
2219 1735 1578 804 617 539
BsaAI yAC'GTr
Cuts at: 493 1204 3229 5283 493
Size: 711 2025 2054 2702
Fragments arranged by size:
2702 2054 2025 711
BsaBI GATnn'nnATC
Cuts at: 4250 7486 4250
Size: 3236 4256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 2111 2542 3318
Size: 53 83 186 936 555 23 431 776
Cuts at: 3318 3375 3707 4164 5299 5681 6316 275
Size: 57 332 457 1135 382 635 1451
Fragments arranged by size:
1451 1135 936 776 635 555 457 431
382 332 186 83 57 53 23
BsaWI w'CCGG_w
Cuts at: 2253 3327 5925 6323 6597 6744 7155 2253
Size: 1074 2598 398 274 147 411 2590
Fragments arranged by size:
2598 2590 1074 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7267
Fragments arranged by size:
7267 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2702 2849 3248 1186
Size: 1516 147 399 5430
Fragments arranged by size:
5430 1516 399 147
BseYI C'CCAG_C
Cuts at: 2701 3261 3660 4024 4270 4442 6695 7191 2701
Size: 560 399 364 246 172 2253 496 3002
Fragments arranged by size:
3002 2253 560 496 399 364 246 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2251 3818 3890 3893 3968 4350 4418
Size: 573 515 1567 72 3 75 382 68
Cuts at: 4418 4472 1163
Size: 54 4183
Fragments arranged by size:
4183 1567 573 515 382 75 72 68
54 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3328 4198 4942 5703 6731 1540
Size: 275 186 1327 870 744 761 1028 2301
Fragments arranged by size:
2301 1327 1028 870 761 744 275 186
BsiHKAI G_wGCw'C
Cuts at: 2298 2421 2699 3039 3645 3774 4065 4212 5553
Size: 123 278 340 606 129 291 147 1341
Cuts at: 5553 5638 6709 2298
Size: 85 1071 3081
Fragments arranged by size:
3081 1341 1071 606 340 291 278 147
129 123 85
BsmI GAATG_Cn'
Cuts at: 5003 5096 5003
Size: 93 7399
BsmBI CGTCTCn'nnnn_
Cuts at: 4458 4775 4458
Size: 317 7175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2227 2298 2421 2699 3006 3039
Size: 399 373 64 71 123 278 307 33
Cuts at: 3039 3282 3617 3645 3660 3774 3816 4031 4065
Size: 243 335 28 15 114 42 215 34
Cuts at: 4065 4075 4101 4204 4212 4811 5553 5638 6709
Size: 10 26 103 8 599 742 85 1071
Cuts at: 6709 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 599 399 373 335 307
278 243 215 123 114 103 85 71
64 42 34 33 28 26 15 10
8
BspEI T'CCGG_A
Cuts at: 2253 7155 2253
Size: 4902 2590
BspHI T'CATG_A
Cuts at: 2883 4659 5381 7111 2883
Size: 1776 722 1730 3264
Fragments arranged by size:
3264 1776 1730 722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3051 3715 3790 4539 4847 844
Size: 2207 664 75 749 308 3489
Fragments arranged by size:
3489 2207 749 664 308 75
BsrBI CCG'CTC
Cuts at: 1394 1823 2245 2881 3745 5379 1394
Size: 429 422 636 864 1634 3507
Fragments arranged by size:
3507 1634 864 636 429 422
BsrDI GCAATG_nn'
Cuts at: 65 1248 5987 6161 65
Size: 1183 4739 174 1396
Fragments arranged by size:
4739 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2090 3327 3922 4743 6136 6323 1700
Size: 110 280 1237 595 821 1393 187 2869
Fragments arranged by size:
2869 1393 1237 821 595 280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7492
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7492
BssSI C'ACGA_G
Cuts at: 2629 3398 5552 6564 2629
Size: 769 2154 1012 3557
Fragments arranged by size:
3557 2154 1012 769
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3043 3925 4626 794
Size: 2249 882 701 3660
Fragments arranged by size:
3660 2249 882 701
BstBI TT'CG_AA
Cuts at: 6296 6296
Size: 7492
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7492
BstXI CCAn_nnnn'nTGG
Cuts at: 3505 3505
Size: 7492
BstYI r'GATC_y
Cuts at: 1972 2250 2739 3048 3084 3216 3555 4554 4822
Size: 278 489 309 36 132 339 999 268
Cuts at: 4822 5189 5574 5591 7043 7129 7141 7487 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 999 489 385 367 346 339
309 278 268 132 86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2832 3300 2832
Size: 468 7024
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 2114 2718 3867 513
Size: 552 939 8 102 604 1149 4138
Fragments arranged by size:
4138 1149 939 604 552 102 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3829 487
Size: 683 56 2603 4150
Fragments arranged by size:
4150 2603 683 56
BtsI GCAGTG_nn'
Cuts at: 3761 4170 4349 5008 5802 5822 3761
Size: 409 179 659 794 20 5431
Fragments arranged by size:
5431 794 659 409 179 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7492
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3166 3201 529
Size: 35 2602 35 4820
Fragments arranged by size:
4820 2602 35 35
DraI TTT'AAA
Cuts at: 4796 4796
Size: 7492
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2317 3380 6499 809
Size: 507 1001 1063 3119 1802
Fragments arranged by size:
3119 1802 1063 1001 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2616 3507 3714
Size: 54 1425 50 275 18 786 891 207
Cuts at: 3714 4725 4939 5828 7457 8
Size: 1011 214 889 1629 43
Fragments arranged by size:
1629 1425 1011 891 889 786 275 214
207 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4939 1537
Size: 275 3127 4090
Fragments arranged by size:
4090 3127 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2159 2786 4377 5422 1353
Size: 806 627 1591 1045 3423
Fragments arranged by size:
3423 1591 1045 806 627
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2050 2199 3747 3904 6079 6453
Size: 1064 138 461 149 1548 157 2175 374
Cuts at: 6453 6599 387
Size: 146 1280
Fragments arranged by size:
2175 1548 1280 1064 461 374 157 149
146 138
EcoICRI GAG'CTC
Cuts at: 4063 4063
Size: 7492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2290 2462 2473 2866 3038 3134
Size: 393 542 116 172 11 393 172 96
Cuts at: 3134 3343 3410 4073 4131 4409 4757 5555 6152
Size: 209 67 663 58 278 348 798 597
Cuts at: 6152 6939 7207 1239
Size: 787 268 1524
Fragments arranged by size:
1524 798 787 663 597 542 393 393
348 278 268 209 172 172 116 96
67 58 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2806 2993 2806
Size: 187 7305
EcoO109I rG'GnC_Cy
Cuts at: 2223 2224 2757 2828 3002 3265 3296 3502 3562
Size: 1 533 71 174 263 31 206 60
Cuts at: 3562 3979 4650 2223
Size: 417 671 5065
Fragments arranged by size:
5065 671 533 417 263 206 174 71
60 31 1
EcoRI G'AATT_C
Cuts at: 4801 5179 4801
Size: 378 7114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 2488 2611 4171 2152
Size: 336 123 1560 5473
Fragments arranged by size:
5473 1560 336 123
FspI TGC'GCA
Cuts at: 2398 3854 3941 5998 2398
Size: 1456 87 2057 3892
Fragments arranged by size:
3892 2057 1456 87
HaeII r_GCGC'y
Cuts at: 1678 2114 2137 3017 3321 3897 6639 1678
Size: 436 23 880 304 576 2742 2531
Fragments arranged by size:
2742 2531 880 576 436 304 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 2841 2873 3194
Size: 32 135 32 464 32 773 32 321
Cuts at: 3194 3226 3742 3774 6133 6165 6207 6239 1373
Size: 32 516 32 2359 32 42 32 2626
Fragments arranged by size:
2626 2359 773 516 464 321 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4836 5082 669
Size: 988 281 2898 246 3079
Fragments arranged by size:
3079 2898 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3821 748
Size: 3073 4419
HpaI GTT'AAC
Cuts at: 5082 5082
Size: 7492
KasI G'GCGC_C
Cuts at: 2110 3317 2110
Size: 1207 6285
KpnI G_GTAC'C
Cuts at: 4817 4817
Size: 7492
MfeI C'AATT_G
Cuts at: 5091 5091
Size: 7492
MluI A'CGCG_T
Cuts at: 7368 7368
Size: 7492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6606 6790 1305
Size: 5301 184 2007
Fragments arranged by size:
5301 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2618 3509 10
Size: 54 1425 343 786 891 3993
Fragments arranged by size:
3993 1425 891 786 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3521 3794 3869 3947 3972 4034 5450
Size: 648 2355 273 75 78 25 62 1416
Cuts at: 5450 5809 5968 518
Size: 359 159 2042
Fragments arranged by size:
2355 2042 1416 648 359 273 159 78
75 62 25
MspA1I CmG'CkG
Cuts at: 1606 2014 2073 2116 2440 2460 2625 2844 3253
Size: 408 59 43 324 20 165 219 409
Cuts at: 3253 3261 3664 4024 4604 4696 4768 4908 5585
Size: 8 403 360 580 92 72 140 677
Cuts at: 5585 6733 6978 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 580 409 408 403 360
324 245 219 165 140 92 72 59
43 20 8
NarI GG'CG_CC
Cuts at: 2111 3318 2111
Size: 1207 6285
NcoI C'CATG_G
Cuts at: 513 1065 2004 3867 513
Size: 552 939 1863 4138
Fragments arranged by size:
4138 1863 939 552
NdeI CA'TA_TG
Cuts at: 387 2287 387
Size: 1900 5592
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7492
NotI GC'GGCC_GC
Cuts at: 4939 4939
Size: 7492
NruI TCG'CGA
Cuts at: 3762 3762
Size: 7492
NspI r_CATG'y
Cuts at: 1132 1229 3967 4131 4850 6395 1132
Size: 97 2738 164 719 1545 2229
Fragments arranged by size:
2738 2229 1545 719 164 97
PasI CC'CwG_GG
Cuts at: 1348 2219 3145 3624 3635 4609 1348
Size: 871 926 479 11 974 4231
Fragments arranged by size:
4231 974 926 871 479 11
PciI A'CATG_T
Cuts at: 6391 6391
Size: 7492
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2197 3624 1108
Size: 1089 1427 4976
Fragments arranged by size:
4976 1427 1089
PfoI T'CCnGG_A
Cuts at: 2489 3558 4158 4254 4425 2489
Size: 1069 600 96 171 5556
Fragments arranged by size:
5556 1069 600 171 96
PmeI GTTT'AAAC
Cuts at: 4796 4796
Size: 7492
PmlI CAC'GTG
Cuts at: 3229 3229
Size: 7492
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5561 5593 7074 7106 5561
Size: 32 1481 32 5947
Fragments arranged by size:
5947 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3265 3502 3562 3265
Size: 237 60 7195
Fragments arranged by size:
7195 237 60
PshAI GACnn'nnGTC
Cuts at: 1613 2091 1613
Size: 478 7014
PsiI TTA'TAA
Cuts at: 5062 5062
Size: 7492
PspOMI G'GGCC_C
Cuts at: 2223 3002 2223
Size: 779 6713
PspXI vC'TCGA_Gb
Cuts at: 3683 3683
Size: 7492
PstI C_TGCA'G
Cuts at: 830 2373 2679 3726 4844 830
Size: 1543 306 1047 1118 3478
Fragments arranged by size:
3478 1543 1118 1047 306
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7492
PvuII CAG'CTG
Cuts at: 2440 2625 3253 2440
Size: 185 628 6679
Fragments arranged by size:
6679 628 185
SacI G_AGCT'C
Cuts at: 4065 4065
Size: 7492
SacII CC_GC'GG
Cuts at: 2015 2117 2015
Size: 102 7390
SalI G'TCGA_C
Cuts at: 1936 4834 1936
Size: 2898 4594
SapI GCTCTTCn'nnn_
Cuts at: 2159 4377 2159
Size: 2218 5274
SbfI CC_TGCA'GG
Cuts at: 4844 4844
Size: 7492
ScaI AGT'ACT
Cuts at: 2351 5740 7452 2351
Size: 3389 1712 2391
Fragments arranged by size:
3389 2391 1712
SfcI C'TryA_G
Cuts at: 826 1045 2369 2675 3722 4186 4840 5975 6656
Size: 219 1324 306 1047 464 654 1135 681
Cuts at: 6656 6847 826
Size: 191 1471
Fragments arranged by size:
1471 1324 1135 1047 681 654 464 306
219 191
SfoI GGC'GCC
Cuts at: 2112 3319 2112
Size: 1207 6285
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7492
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7492
SmaI CCC'GGG
Cuts at: 1872 2142 4819 1872
Size: 270 2677 4545
Fragments arranged by size:
4545 2677 270
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3405 3683 4004 4374 5596 6497
Size: 197 926 1462 278 321 370 1222 901
Cuts at: 6497 6759 820
Size: 262 1553
Fragments arranged by size:
1553 1462 1222 926 901 370 321 278
262 197
SnaBI TAC'GTA
Cuts at: 493 5283 493
Size: 4790 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7492
SphI G_CATG'C
Cuts at: 1132 3967 4850 1132
Size: 2835 883 3774
Fragments arranged by size:
3774 2835 883
SspI AAT'ATT
Cuts at: 5 52 5416 5
Size: 47 5364 2081
Fragments arranged by size:
5364 2081 47
StuI AGG'CCT
Cuts at: 1517 4013 1517
Size: 2496 4996
StyI C'CwwG_G
Cuts at: 513 1065 2004 3006 3498 3783 3867 4931 513
Size: 552 939 1002 492 285 84 1064 3074
Fragments arranged by size:
3074 1064 1002 939 552 492 285 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3321 4212 4351 5519 5536 5689 5874
Size: 1255 1741 891 139 1168 17 153 185
Cuts at: 5874 6319 325
Size: 445 1498
Fragments arranged by size:
1741 1498 1255 1168 891 445 185 153
139 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2258 2349 5738
Size: 274 80 33 51 1443 281 91 3389
Cuts at: 5738 7450 96
Size: 1712 138
Fragments arranged by size:
3389 1712 1443 281 274 138 91 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2540 2572 7452 7484 2540
Size: 32 4880 32 2548
Fragments arranged by size:
4880 2548 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7492
XbaI T'CTAG_A
Cuts at: 4828 4828
Size: 7492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3013 3142 3709 3013
Size: 129 567 6796
Fragments arranged by size:
6796 567 129
XhoI C'TCGA_G
Cuts at: 1943 3405 3683 1943
Size: 1462 278 5752
Fragments arranged by size:
5752 1462 278
XmaI C'CCGG_G
Cuts at: 1870 2140 4817 1870
Size: 270 2677 4545
Fragments arranged by size:
4545 2677 270
XmnI GAAnn'nnTTC
Cuts at: 2576 4412 5621 2576
Size: 1836 1209 4447
Fragments arranged by size:
4447 1836 1209
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3708 5300 276
Size: 53 83 186 936 2174 1592 2468
Fragments arranged by size:
2468 2174 1592 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NsiI
PacI PsrI RsrII SanDI SexAI SfiI SrfI SwaI