Restriction map for
pFN21AE3501
(Circular) (Six-base) MAPSORT of: pFN21AE3501.seq Check: 172 from: 1 to: 8371
pFN21AE3501 8371 bp
With 182 enzymes: SgfI *
March 28, 2012 12:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4288 4288
Size: 8371
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6181 278
Size: 53 83 186 936 4645 2468
Fragments arranged by size:
4645 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5714 1937
Size: 3777 4594
Acc65I G'GTAC_C
Cuts at: 3583 5692 3583
Size: 2109 6262
AclI AA'CG_TT
Cuts at: 1563 6498 6871 1563
Size: 4935 373 3063
Fragments arranged by size:
4935 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4388 4590 4643 4890 5133 5447 6434 7818
Size: 2756 202 53 247 243 314 987 1384
Cuts at: 7818 8086 1632
Size: 268 1917
Fragments arranged by size:
2756 1917 1384 987 314 268 247 243
202 53
AflII C'TTAA_G
Cuts at: 820 1017 2716 4376 820
Size: 197 1699 1660 4815
Fragments arranged by size:
4815 1699 1660 197
AflIII A'CryG_T
Cuts at: 1203 4752 7270 8247 1203
Size: 3549 2518 977 1327
Fragments arranged by size:
3549 2518 1327 977
AgeI A'CCGG_T
Cuts at: 7202 7202
Size: 8371
AhdI GACnn_n'nnGTC
Cuts at: 7100 7100
Size: 8371
AleI CACnn'nnGTG
Cuts at: 1166 3638 1166
Size: 2472 5899
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3013 3656 3984 1184
Size: 1829 643 328 5571
Fragments arranged by size:
5571 1829 643 328
AlwNI CAG_nnn'CTG
Cuts at: 2251 2752 3371 6114 7686 2251
Size: 501 619 2743 1572 2936
Fragments arranged by size:
2936 2743 1572 619 501
ApaLI G'TGCA_C
Cuts at: 2998 6428 7584 2998
Size: 3430 1156 3785
Fragments arranged by size:
3785 3430 1156
ApoI r'AATT_y
Cuts at: 1493 3118 3914 4424 4551 5509 5593 5680 5906
Size: 1625 796 510 127 958 84 87 226
Cuts at: 5906 6058 8167 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1625 958 796 510 226 152
127 87 84
AseI AT'TA_AT
Cuts at: 160 1032 4709 6925 160
Size: 872 3677 2216 1606
Fragments arranged by size:
3677 2216 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8371
AvaI C'yCGr_G
Cuts at: 1870 1943 2315 2679 5696 1870
Size: 73 372 364 3017 4545
Fragments arranged by size:
4545 3017 372 364 73
BamHI G'GATC_C
Cuts at: 2155 5701 6068 2155
Size: 3546 367 4458
Fragments arranged by size:
4458 3546 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2329 3422 3583 3624 5370
Size: 325 217 627 542 1093 161 41 1746
Cuts at: 5370 5692 7147 618
Size: 322 1455 1842
Fragments arranged by size:
1842 1746 1455 1093 627 542 325 322
217 161 41
BanII G_rGCy'C
Cuts at: 1391 2686 2703 2982 5690 1391
Size: 1295 17 279 2708 4072
Fragments arranged by size:
4072 2708 1295 279 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4374 5453 928
Size: 971 2475 1079 3846
Fragments arranged by size:
3846 2475 1079 971
BbvCI CC'TCA_GC
Cuts at: 3509 3509
Size: 8371
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6562 6596 1903
Size: 34 4625 34 3678
Fragments arranged by size:
4625 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3293 6263 7473 3293
Size: 2970 1210 4191
Fragments arranged by size:
4191 2970 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8371
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6982 136
Size: 107 122 71 631 605 5310 1525
Fragments arranged by size:
5310 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8366 8366
Size: 8371
BlpI GC'TnA_GC
Cuts at: 5788 5788
Size: 8371
Bme1580I G_kGCm'C
Cuts at: 1790 2332 3002 3427 6432 7588 1790
Size: 542 670 425 3005 1156 2573
Fragments arranged by size:
3005 2573 1156 670 542 425
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8371
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2501 3012 4242
Size: 416 53 83 399 24 1084 511 1230
Cuts at: 4242 5561 6102 7060 442
Size: 1319 541 958 1753
Fragments arranged by size:
1753 1319 1230 1084 958 541 511 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2609 3354 3432 3486 5283 7031 1239
Size: 1370 745 78 54 1797 1748 2579
Fragments arranged by size:
2579 1797 1748 1370 745 78 54
Bpu10I CC'TnA_GC
Cuts at: 3509 3509
Size: 8371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6496 7361 7659 6496
Size: 865 298 7208
Fragments arranged by size:
7208 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2118 7034 882
Size: 617 619 4916 2219
Fragments arranged by size:
4916 2219 619 617
BsaAI yAC'GTr
Cuts at: 493 1204 6162 493
Size: 711 4958 2702
Fragments arranged by size:
4958 2702 711
BsaBI GATnn'nnATC
Cuts at: 3309 4337 8365 3309
Size: 1028 4028 3315
Fragments arranged by size:
4028 3315 1028
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2087 6178 6560 7195
Size: 53 83 186 936 554 4091 382 635
Cuts at: 7195 275
Size: 1451
Fragments arranged by size:
4091 1451 936 635 554 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2127 2192 2558 3053 6804 7202 7476 7623 8034
Size: 65 366 495 3751 398 274 147 411
Cuts at: 8034 2127
Size: 2464
Fragments arranged by size:
3751 2464 495 411 398 366 274 147
65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4228 4258 4282 4312 4707
Size: 30 165 30 2825 30 24 30 395
Cuts at: 4707 4737 1178
Size: 30 4812
Fragments arranged by size:
4812 2825 395 165 30 30 30 30
30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2040 2276 3669 4133 5618 1186
Size: 854 236 1393 464 1485 3939
Fragments arranged by size:
3939 1485 1393 854 464 236
BseYI C'CCAG_C
Cuts at: 2251 5316 7574 8070 2251
Size: 3065 2258 496 2552
Fragments arranged by size:
3065 2552 2258 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4260 1163
Size: 573 2524 5274
Fragments arranged by size:
5274 2524 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5821 6582 7610 1540
Size: 275 186 3820 761 1028 2301
Fragments arranged by size:
3820 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2703 3002 4018 6432 6517 7588 2703
Size: 299 1016 2414 85 1071 3486
Fragments arranged by size:
3486 2414 1071 1016 299 85
BsmI GAATG_Cn'
Cuts at: 5092 5882 5975 5092
Size: 790 93 7488
Fragments arranged by size:
7488 790 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5274 5274
Size: 8371
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2332 2686 2703 2982 3002 3427 4018
Size: 399 542 354 17 279 20 425 591
Cuts at: 4018 5690 6432 6517 7588 1391
Size: 1672 742 85 1071 2174
Fragments arranged by size:
2174 1672 1071 742 591 542 425 399
354 279 85 20 17
BspEI T'CCGG_A
Cuts at: 2558 8034 2558
Size: 5476 2895
BspHI T'CATG_A
Cuts at: 6260 7990 6260
Size: 1730 6641
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2304 4288 5726 844
Size: 1460 1984 1438 3489
Fragments arranged by size:
3489 1984 1460 1438
BsrBI CCG'CTC
Cuts at: 1394 1823 2556 6258 1394
Size: 429 733 3702 3507
Fragments arranged by size:
3702 3507 733 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3888 6866 7040 65
Size: 1183 2640 2978 174 1396
Fragments arranged by size:
2978 2640 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7015 7202 1700
Size: 110 5205 187 2869
Fragments arranged by size:
5205 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8371
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8371
BssSI C'ACGA_G
Cuts at: 2234 6431 7443 2234
Size: 4197 1012 3162
Fragments arranged by size:
4197 3162 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2248 5564 794
Size: 1454 3316 3601
Fragments arranged by size:
3601 3316 1454
BstBI TT'CG_AA
Cuts at: 5251 7175 5251
Size: 1924 6447
BstEII G'GTnAC_C
Cuts at: 1184 2266 2495 4369 5114 1184
Size: 1082 229 1874 745 4441
Fragments arranged by size:
4441 1874 1082 745 229
BstXI CCAn_nnnn'nTGG
Cuts at: 2882 4031 4804 2882
Size: 1149 773 6449
Fragments arranged by size:
6449 1149 773
BstYI r'GATC_y
Cuts at: 1972 2155 4332 5701 6068 6453 6470 7922 8008
Size: 183 2177 1369 367 385 17 1452 86
Cuts at: 8008 8020 8366 1972
Size: 12 346 1977
Fragments arranged by size:
2177 1977 1452 1369 385 367 346 183
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2058 2844 2864 3147 3354 2058
Size: 786 20 283 207 7075
Fragments arranged by size:
7075 786 283 207 20
BtgI C'CryG_G
Cuts at: 513 1065 3628 4365 4797 5598 513
Size: 552 2563 737 432 801 3286
Fragments arranged by size:
3286 2563 801 737 552 432
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7632
Fragments arranged by size:
7632 683 56
BtsI GCAGTG_nn'
Cuts at: 2380 2656 5887 6681 6701 2380
Size: 276 3231 794 20 4050
Fragments arranged by size:
4050 3231 794 276 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8371
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8336
DraI TTT'AAA
Cuts at: 5675 5675
Size: 8371
DraIII CAC_nnn'GTG
Cuts at: 4609 4609
Size: 8371
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2077 7378 809
Size: 507 761 5301 1802
Fragments arranged by size:
5301 1802 761 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2224 5818 6707
Size: 54 1425 50 275 18 394 3594 889
Cuts at: 6707 8336 8
Size: 1629 43
Fragments arranged by size:
3594 1629 1425 889 394 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5818 1537
Size: 275 4006 4090
Fragments arranged by size:
4090 4006 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2708 3174 3799 5607 6301 1353
Size: 1355 466 625 1808 694 3423
Fragments arranged by size:
3423 1808 1355 694 625 466
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6958 7332 7478 387
Size: 1064 138 5369 374 146 1280
Fragments arranged by size:
5369 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2701 2701
Size: 8371
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2609 3354 3432 3486 4388 4590 4643
Size: 393 977 745 78 54 902 202 53
Cuts at: 4643 4890 5133 5283 5447 6434 7031 7818 8086
Size: 247 243 150 164 987 597 787 268
Cuts at: 8086 1239
Size: 1524
Fragments arranged by size:
1524 987 977 902 787 745 597 393
268 247 243 202 164 150 78 54
53
EcoO109I rG'GnC_Cy
Cuts at: 3151 3151
Size: 8371
EcoRI G'AATT_C
Cuts at: 4424 5680 6058 4424
Size: 1256 378 6737
Fragments arranged by size:
6737 1256 378
EcoRV GAT'ATC
Cuts at: 2762 2762
Size: 8371
FspI TGC'GCA
Cuts at: 6877 6877
Size: 8371
HaeII r_GCGC'y
Cuts at: 1678 7518 1678
Size: 5840 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4228 4253 4260 4285 4712
Size: 32 135 32 2656 25 7 25 427
Cuts at: 4712 4744 5608 5640 7012 7044 7086 7118 1373
Size: 32 864 32 1372 32 42 32 2626
Fragments arranged by size:
2656 2626 1372 864 427 135 42 32
32 32 32 32 32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2530 5715 5961 669
Size: 988 281 592 3185 246 3079
Fragments arranged by size:
3185 3079 988 592 281 246
HindIII A'AGCT_T
Cuts at: 748 2444 3774 748
Size: 1696 1330 5345
Fragments arranged by size:
5345 1696 1330
HpaI GTT'AAC
Cuts at: 2530 5961 2530
Size: 3431 4940
KpnI G_GTAC'C
Cuts at: 3587 5696 3587
Size: 2109 6262
MfeI C'AATT_G
Cuts at: 5970 5970
Size: 8371
MluI A'CGCG_T
Cuts at: 8247 8247
Size: 8371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2397 4012 7485 7669 1305
Size: 1092 1615 3473 184 2007
Fragments arranged by size:
3473 2007 1615 1092 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6549
Fragments arranged by size:
6549 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2633 3638 4802 6329 6688 6847 518
Size: 648 1467 1005 1164 1527 359 159 2042
Fragments arranged by size:
2042 1527 1467 1164 1005 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2230 2248 3371 3508 5787 6464 7612 7857
Size: 624 18 1123 137 2279 677 1148 245
Cuts at: 7857 1606
Size: 2120
Fragments arranged by size:
2279 2120 1148 1123 677 624 245 137
18
NcoI C'CATG_G
Cuts at: 513 1065 3628 4365 4797 513
Size: 552 2563 737 432 4087
Fragments arranged by size:
4087 2563 737 552 432
NdeI CA'TA_TG
Cuts at: 387 3559 387
Size: 3172 5199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8371
NotI GC'GGCC_GC
Cuts at: 5818 5818
Size: 8371
NspI r_CATG'y
Cuts at: 1132 1229 2528 2642 2672 2714 4756 5729 7274
Size: 97 1299 114 30 42 2042 973 1545
Cuts at: 7274 1132
Size: 2229
Fragments arranged by size:
2229 2042 1545 1299 973 114 97 42
30
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8371
PciI A'CATG_T
Cuts at: 4752 7270 4752
Size: 2518 5853
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3593 1108
Size: 2485 5886
PfoI T'CCnGG_A
Cuts at: 2141 2451 3463 5260 2141
Size: 310 1012 1797 5252
Fragments arranged by size:
5252 1797 1012 310
PmeI GTTT'AAAC
Cuts at: 5675 5675
Size: 8371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6440 6472 7953 7985 6440
Size: 32 1481 32 6826
Fragments arranged by size:
6826 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3151 3151
Size: 8371
PshAI GACnn'nnGTC
Cuts at: 1613 4360 1613
Size: 2747 5624
PsiI TTA'TAA
Cuts at: 5941 5941
Size: 8371
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4944 4976 4944
Size: 32 8339
PstI C_TGCA'G
Cuts at: 830 2992 4591 4903 5723 830
Size: 2162 1599 312 820 3478
Fragments arranged by size:
3478 2162 1599 820 312
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8371
PvuII CAG'CTG
Cuts at: 2248 3371 3508 2248
Size: 1123 137 7111
Fragments arranged by size:
7111 1123 137
SacI G_AGCT'C
Cuts at: 2703 2703
Size: 8371
SalI G'TCGA_C
Cuts at: 1936 5713 1936
Size: 3777 4594
SapI GCTCTTCn'nnn_
Cuts at: 2708 2708
Size: 8371
SbfI CC_TGCA'GG
Cuts at: 5723 5723
Size: 8371
ScaI AGT'ACT
Cuts at: 3496 6619 8331 3496
Size: 3123 1712 3536
Fragments arranged by size:
3536 3123 1712
SfcI C'TryA_G
Cuts at: 826 1045 2988 3322 4587 4777 4899 5719 6854
Size: 219 1943 334 1265 190 122 820 1135
Cuts at: 6854 7535 7726 826
Size: 681 191 1471
Fragments arranged by size:
1943 1471 1265 1135 820 681 334 219
191 190 122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8371
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8371
SmaI CCC'GGG
Cuts at: 1872 5698 1872
Size: 3826 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2716 4376 6475 7376 7638 820
Size: 197 926 773 1660 2099 901 262 1553
Fragments arranged by size:
2099 1660 1553 926 901 773 262 197
SnaBI TAC'GTA
Cuts at: 493 6162 493
Size: 5669 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8371
SphI G_CATG'C
Cuts at: 1132 2672 2714 5729 1132
Size: 1540 42 3015 3774
Fragments arranged by size:
3774 3015 1540 42
SspI AAT'ATT
Cuts at: 5 52 4162 6295 5
Size: 47 4110 2133 2081
Fragments arranged by size:
4110 2133 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8371
StyI C'CwwG_G
Cuts at: 513 1065 2082 2856 3075 3628 4293 4365 4797
Size: 552 1017 774 219 553 665 72 432
Cuts at: 4797 5810 513
Size: 1013 3074
Fragments arranged by size:
3074 1017 1013 774 665 553 552 432
219 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2285 2347 2483 6398 6415 6568 6753
Size: 1255 705 62 136 3915 17 153 185
Cuts at: 6753 7198 325
Size: 445 1498
Fragments arranged by size:
3915 1498 1255 705 445 185 153 136
62 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3165 3494 4683
Size: 274 80 33 51 1443 1188 329 1189
Cuts at: 4683 4823 5387 6617 8329 96
Size: 140 564 1230 1712 138
Fragments arranged by size:
1712 1443 1230 1189 1188 564 329 274
140 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2147 2179 5583 5615 8331 8363 2147
Size: 32 3404 32 2716 32 2155
Fragments arranged by size:
3404 2716 2155 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2074 1316
Size: 758 7613
XbaI T'CTAG_A
Cuts at: 5707 5707
Size: 8371
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8371
XmaI C'CCGG_G
Cuts at: 1870 5696 1870
Size: 3826 4545
XmnI GAAnn'nnTTC
Cuts at: 2137 3694 4515 5484 6500 2137
Size: 1557 821 969 1016 4008
Fragments arranged by size:
4008 1557 1016 969 821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6179 276
Size: 53 83 186 936 4645 2468
Fragments arranged by size:
4645 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI