Restriction map for
pFN21AE3492
(Circular) (Six-base) MAPSORT of: pFN21AE3492.seq Check: 2508 from: 1 to: 10234
pFN21AE3492 10234 bp
With 182 enzymes: SgfI *
June 11, 2014 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6737 6737
Size: 10234
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6109 8044 278
Size: 53 83 186 936 4573 1935 2468
Fragments arranged by size:
4573 2468 1935 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2740 6919 7408 7577 1937
Size: 803 4179 489 169 4594
Fragments arranged by size:
4594 4179 803 489 169
Acc65I G'GTAC_C
Cuts at: 7555 7555
Size: 10234
AclI AA'CG_TT
Cuts at: 1563 2075 4712 8361 8734 1563
Size: 512 2637 3649 373 3063
Fragments arranged by size:
3649 3063 2637 512 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2670 3551 3906 5231 5246 5309 5502 5576
Size: 1038 881 355 1325 15 63 193 74
Cuts at: 5576 5808 6284 6318 6547 7224 8297 9681 9949
Size: 232 476 34 229 677 1073 1384 268
Cuts at: 9949 1632
Size: 1917
Fragments arranged by size:
1917 1384 1325 1073 1038 881 677 476
355 268 232 229 193 74 63 34
15
AflII C'TTAA_G
Cuts at: 820 1017 3712 4214 4898 820
Size: 197 2695 502 684 6156
Fragments arranged by size:
6156 2695 684 502 197
AflIII A'CryG_T
Cuts at: 1203 9133 10110 1203
Size: 7930 977 1327
Fragments arranged by size:
7930 1327 977
AgeI A'CCGG_T
Cuts at: 9065 9065
Size: 10234
AhdI GACnn_n'nnGTC
Cuts at: 8963 8963
Size: 10234
AleI CACnn'nnGTG
Cuts at: 1166 6786 1166
Size: 5620 4614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6642 6674 6642
Size: 32 10202
AlwNI CAG_nnn'CTG
Cuts at: 5714 7442 7977 9549 5714
Size: 1728 535 1572 6399
Fragments arranged by size:
6399 1728 1572 535
ApaLI G'TGCA_C
Cuts at: 8291 9447 8291
Size: 1156 9078
ApoI r'AATT_y
Cuts at: 1493 2016 2264 2423 2436 2893 3023 3580 3646
Size: 523 248 159 13 457 130 557 66
Cuts at: 3646 3774 3824 3997 4090 4585 5335 5812 5867
Size: 128 50 173 93 495 750 477 55
Cuts at: 5867 5959 5994 6149 6451 6794 7498 7543 7769
Size: 92 35 155 302 343 704 45 226
Cuts at: 7769 7921 10030 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 750 704 557 523 495 477
457 343 302 248 226 173 159 155
152 130 128 93 92 66 55 50
45 35 13
AseI AT'TA_AT
Cuts at: 160 1032 2169 3227 5147 6005 6234 8788 160
Size: 872 1137 1058 1920 858 229 2554 1606
Fragments arranged by size:
2554 1920 1606 1137 1058 872 858 229
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10234
AvaI C'yCGr_G
Cuts at: 1870 1943 7559 1870
Size: 73 5616 4545
Fragments arranged by size:
5616 4545 73
BamHI G'GATC_C
Cuts at: 7564 7931 7564
Size: 367 9867
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7555 9010 618
Size: 325 217 627 5768 1455 1842
Fragments arranged by size:
5768 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4233 5063 7553 1391
Size: 2842 830 2490 4072
Fragments arranged by size:
4072 2842 2490 830
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3063 3102 3557 3699 4760 5088 5394
Size: 971 1164 39 455 142 1061 328 306
Cuts at: 5394 5451 5607 5816 6615 928
Size: 57 156 209 799 4547
Fragments arranged by size:
4547 1164 1061 971 799 455 328 306
209 156 142 57 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8425 8459 1903
Size: 34 6488 34 3678
Fragments arranged by size:
6488 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8126 9336 8126
Size: 1210 9024
BclI T'GATC_A
Cuts at: 1548 2295 5472 1548
Size: 747 3177 6310
Fragments arranged by size:
6310 3177 747
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8845 136
Size: 107 122 71 631 605 7173 1525
Fragments arranged by size:
7173 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 10229 10229
Size: 10234
BlpI GC'TnA_GC
Cuts at: 3612 3812 4167 5942 7651 3612
Size: 200 355 1775 1709 6195
Fragments arranged by size:
6195 1775 1709 355 200
Bme1580I G_kGCm'C
Cuts at: 1790 8295 9451 1790
Size: 6505 1156 2573
Fragments arranged by size:
6505 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10234
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6875 7965 8923
Size: 416 53 83 399 24 5458 1090 958
Cuts at: 8923 442
Size: 1753
Fragments arranged by size:
5458 1753 1090 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10234
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5082 5082
Size: 10234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2700 6268 6872 6932 8894 1239
Size: 815 646 3568 604 60 1962 2579
Fragments arranged by size:
3568 2579 1962 815 646 604 60
Bpu10I CC'TnA_GC
Cuts at: 2792 2792
Size: 10234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2867 4820 5240 5597 7006 7236 8359 9224 9522
Size: 1953 420 357 1409 230 1123 865 298
Cuts at: 9522 2867
Size: 3579
Fragments arranged by size:
3579 1953 1409 1123 865 420 357 298
230
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2669 5586 7177 8897 882
Size: 617 1170 2917 1591 1720 2219
Fragments arranged by size:
2917 2219 1720 1591 1170 617
BsaAI yAC'GTr
Cuts at: 493 1204 4727 8025 493
Size: 711 3523 3298 2702
Fragments arranged by size:
3523 3298 2702 711
BsaBI GATnn'nnATC
Cuts at: 6234 10228 6234
Size: 3994 6240
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6106 8041 8423 9058
Size: 53 83 186 936 4573 1935 382 635
Cuts at: 9058 275
Size: 1451
Fragments arranged by size:
4573 1935 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 8667 9065 9339 9486 9897 8667
Size: 398 274 147 411 9004
Fragments arranged by size:
9004 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 10009
Fragments arranged by size:
10009 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5055 6156 1186
Size: 3869 1101 5264
Fragments arranged by size:
5264 3869 1101
BseYI C'CCAG_C
Cuts at: 3294 9437 9933 3294
Size: 6143 496 3595
Fragments arranged by size:
6143 3595 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2418 2679 3756 6428 6771 1163
Size: 573 682 261 1077 2672 343 4626
Fragments arranged by size:
4626 2672 1077 682 573 343 261
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7684 8445 9473 1540
Size: 275 186 5683 761 1028 2301
Fragments arranged by size:
5683 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3121 4233 5063 6888 8295 8380 9451 3121
Size: 1112 830 1825 1407 85 1071 3904
Fragments arranged by size:
3904 1825 1407 1112 1071 830 85
BsmI GAATG_Cn'
Cuts at: 2235 5320 5383 7745 7838 2235
Size: 3085 63 2362 93 4631
Fragments arranged by size:
4631 3085 2362 93 63
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3121 4233 5063 6888 7553 8295 8380
Size: 399 1331 1112 830 1825 665 742 85
Cuts at: 8380 9451 1391
Size: 1071 2174
Fragments arranged by size:
2174 1825 1331 1112 1071 830 742 665
399 85
BspEI T'CCGG_A
Cuts at: 9897 9897
Size: 10234
BspHI T'CATG_A
Cuts at: 2043 2298 3178 3781 4792 5367 5482 5503 5701
Size: 255 880 603 1011 575 115 21 198
Cuts at: 5701 6808 8123 9853 2043
Size: 1107 1315 1730 2424
Fragments arranged by size:
2424 1730 1315 1107 1011 880 603 575
255 198 115 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2529 6737 7500 7589 844
Size: 1685 4208 763 89 3489
Fragments arranged by size:
4208 3489 1685 763 89
BsrBI CCG'CTC
Cuts at: 1394 1823 2656 6276 8121 1394
Size: 429 833 3620 1845 3507
Fragments arranged by size:
3620 3507 1845 833 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2531 2585 3475 4388 8729 8903 65
Size: 1183 1283 54 890 913 4341 174 1396
Fragments arranged by size:
4341 1396 1283 1183 913 890 174 54
BsrFI r'CCGG_y
Cuts at: 1700 1810 8878 9065 1700
Size: 110 7068 187 2869
Fragments arranged by size:
7068 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10234
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10234
BssSI C'ACGA_G
Cuts at: 3114 7329 8294 9306 3114
Size: 4215 965 1012 4042
Fragments arranged by size:
4215 4042 1012 965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2471 794
Size: 1677 8557
BstBI TT'CG_AA
Cuts at: 9038 9038
Size: 10234
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10234
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 6577 6788 2056
Size: 4521 211 5502
Fragments arranged by size:
5502 4521 211
BstYI r'GATC_y
Cuts at: 1972 3592 6052 6566 7564 7931 8316 8333 9785
Size: 1620 2460 514 998 367 385 17 1452
Cuts at: 9785 9871 9883 10229 1972
Size: 86 12 346 1977
Fragments arranged by size:
2460 1977 1620 1452 998 514 385 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 6414 513
Size: 552 939 4410 4333
Fragments arranged by size:
4410 4333 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9495
Fragments arranged by size:
9495 683 56
BtsI GCAGTG_nn'
Cuts at: 5174 6793 6838 7458 7750 8544 8564 5174
Size: 1619 45 620 292 794 20 6844
Fragments arranged by size:
6844 1619 794 620 292 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10234
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10199
DraI TTT'AAA
Cuts at: 3957 4051 5778 7538 3957
Size: 94 1727 1760 6653
Fragments arranged by size:
6653 1760 1727 94
DraIII CAC_nnn'GTG
Cuts at: 2708 4727 6626 2708
Size: 2019 1899 6316
Fragments arranged by size:
6316 2019 1899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2545 9241 809
Size: 507 1229 6696 1802
Fragments arranged by size:
6696 1802 1229 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 6361 7113
Size: 54 1425 50 275 18 258 4273 752
Cuts at: 7113 7681 8570 10199 8
Size: 568 889 1629 43
Fragments arranged by size:
4273 1629 1425 889 752 568 275 258
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7681 1537
Size: 275 5869 4090
Fragments arranged by size:
5869 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2646 2692 3429 5307 6120 6556 7075 8164
Size: 1293 46 737 1878 813 436 519 1089
Cuts at: 8164 1353
Size: 3423
Fragments arranged by size:
3423 1878 1293 1089 813 737 519 436
46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8821 9195 9341 387
Size: 1064 138 7232 374 146 1280
Fragments arranged by size:
7232 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4231 5061 4231
Size: 830 9404
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2670 2700 3551 3906 5231 5246
Size: 393 422 616 30 851 355 1325 15
Cuts at: 5246 5309 5502 5576 5808 6268 6284 6318 6547
Size: 63 193 74 232 460 16 34 229
Cuts at: 6547 6872 6932 7224 8297 8894 9681 9949 1239
Size: 325 60 292 1073 597 787 268 1524
Fragments arranged by size:
1524 1325 1073 851 787 616 597 460
422 393 355 325 292 268 232 229
193 74 63 60 34 30 16 15
EcoNI CCTnn'n_nnAGG
Cuts at: 7242 7242
Size: 10234
EcoRI G'AATT_C
Cuts at: 2436 4090 7543 7921 2436
Size: 1654 3453 378 4749
Fragments arranged by size:
4749 3453 1654 378
EcoRV GAT'ATC
Cuts at: 3312 3312
Size: 10234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3191 3632 4229 6314 7052 3191
Size: 441 597 2085 738 6373
Fragments arranged by size:
6373 2085 738 597 441
FspI TGC'GCA
Cuts at: 8740 8740
Size: 10234
HaeII r_GCGC'y
Cuts at: 1678 9381 1678
Size: 7703 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2897 2929 3362 3394 3426
Size: 32 135 32 1325 32 433 32 32
Cuts at: 3426 4366 4398 5438 5464 5470 5496 5513 5545
Size: 940 32 1040 26 6 26 17 32
Cuts at: 5545 5894 5926 6584 6616 6809 6841 6907 6939
Size: 349 32 658 32 193 32 66 32
Cuts at: 6939 7174 7206 8875 8907 8949 8981 1373
Size: 235 32 1669 32 42 32 2626
Fragments arranged by size:
2626 1669 1325 1040 940 658 433 349
235 193 135 66 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 26 26 17 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2741 3375 5771 6547 6920 7578
Size: 988 281 803 634 2396 776 373 658
Cuts at: 7578 7824 669
Size: 246 3079
Fragments arranged by size:
3079 2396 988 803 776 658 634 373
281 246
HindIII A'AGCT_T
Cuts at: 748 2975 5321 6995 748
Size: 2227 2346 1674 3987
Fragments arranged by size:
3987 2346 2227 1674
HpaI GTT'AAC
Cuts at: 7824 7824
Size: 10234
KpnI G_GTAC'C
Cuts at: 7559 7559
Size: 10234
MfeI C'AATT_G
Cuts at: 2291 4502 4979 5897 7833 2291
Size: 2211 477 918 1936 4692
Fragments arranged by size:
4692 2211 1936 918 477
MluI A'CGCG_T
Cuts at: 10110 10110
Size: 10234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2808 3072 4552 5619 9348 9532 1305
Size: 1503 264 1480 1067 3729 184 2007
Fragments arranged by size:
3729 2007 1503 1480 1067 264 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6363 7115 10
Size: 54 1425 343 4531 752 3129
Fragments arranged by size:
4531 3129 1425 752 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 4160 6786 6813 8192 8551 8710
Size: 648 1083 1911 2626 27 1379 359 159
Cuts at: 8710 518
Size: 2042
Fragments arranged by size:
2626 2042 1911 1379 1083 648 359 159
27
MspA1I CmG'CkG
Cuts at: 1606 2468 3721 4441 7439 7650 8327 9475 9720
Size: 862 1253 720 2998 211 677 1148 245
Cuts at: 9720 1606
Size: 2120
Fragments arranged by size:
2998 2120 1253 1148 862 720 677 245
211
NcoI C'CATG_G
Cuts at: 513 1065 2004 6414 513
Size: 552 939 4410 4333
Fragments arranged by size:
4410 4333 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10234
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10234
NotI GC'GGCC_GC
Cuts at: 7681 7681
Size: 10234
NspI r_CATG'y
Cuts at: 1132 1229 7592 9137 1132
Size: 97 6363 1545 2229
Fragments arranged by size:
6363 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3352 7139 1348
Size: 2004 3787 4443
Fragments arranged by size:
4443 3787 2004
PciI A'CATG_T
Cuts at: 9133 9133
Size: 10234
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5075 5899 6370 1108
Size: 3967 824 471 4972
Fragments arranged by size:
4972 3967 824 471
PmeI GTTT'AAAC
Cuts at: 7538 7538
Size: 10234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3103 3135 8303 8335 9816 9848 3103
Size: 32 5168 32 1481 32 3489
Fragments arranged by size:
5168 3489 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 6615 1613
Size: 5002 5232
PsiI TTA'TAA
Cuts at: 3030 3864 4971 7804 3030
Size: 834 1107 2833 5460
Fragments arranged by size:
5460 2833 1107 834
PstI C_TGCA'G
Cuts at: 830 5719 6559 7465 7586 830
Size: 4889 840 906 121 3478
Fragments arranged by size:
4889 3478 906 840 121
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10234
PvuII CAG'CTG
Cuts at: 4441 7439 4441
Size: 2998 7236
SacI G_AGCT'C
Cuts at: 4233 5063 4233
Size: 830 9404
SalI G'TCGA_C
Cuts at: 1936 2739 6918 7576 1936
Size: 803 4179 658 4594
Fragments arranged by size:
4594 4179 803 658
SapI GCTCTTCn'nnn_
Cuts at: 2646 3429 2646
Size: 783 9451
SbfI CC_TGCA'GG
Cuts at: 7586 7586
Size: 10234
ScaI AGT'ACT
Cuts at: 4849 6445 6854 8482 10194 4849
Size: 1596 409 1628 1712 4889
Fragments arranged by size:
4889 1712 1628 1596 409
SexAI A'CCwGG_T
Cuts at: 2151 6402 7186 2151
Size: 4251 784 5199
Fragments arranged by size:
5199 4251 784
SfcI C'TryA_G
Cuts at: 826 1045 3459 4172 5715 5764 6011 6522 6555
Size: 219 2414 713 1543 49 247 511 33
Cuts at: 6555 7461 7582 8717 9398 9589 826
Size: 906 121 1135 681 191 1471
Fragments arranged by size:
2414 1543 1471 1135 906 713 681 511
247 219 191 121 49 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10234
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10234
SmaI CCC'GGG
Cuts at: 1872 7561 1872
Size: 5689 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2846 3712 4214 4799 4898 5219
Size: 197 926 903 866 502 585 99 321
Cuts at: 5219 5576 7021 7251 8338 9239 9501 820
Size: 357 1445 230 1087 901 262 1553
Fragments arranged by size:
1553 1445 1087 926 903 901 866 585
502 357 321 262 230 197 99
SnaBI TAC'GTA
Cuts at: 493 8025 493
Size: 7532 2702
SpeI A'CTAG_T
Cuts at: 152 3320 152
Size: 3168 7066
SphI G_CATG'C
Cuts at: 1132 7592 1132
Size: 6460 3774
SspI AAT'ATT
Cuts at: 5 52 8158 5
Size: 47 8106 2081
Fragments arranged by size:
8106 2081 47
StuI AGG'CCT
Cuts at: 1517 3983 1517
Size: 2466 7768
StyI C'CwwG_G
Cuts at: 513 1065 2004 6369 6414 7398 7673 513
Size: 552 939 4365 45 984 275 3074
Fragments arranged by size:
4365 3074 984 939 552 275 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6283 8261 8278 8431 8616 9061 325
Size: 1255 4703 1978 17 153 185 445 1498
Fragments arranged by size:
4703 1978 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4847 6078 6443
Size: 274 80 33 51 1443 2870 1231 365
Cuts at: 6443 6852 7006 7483 8480 10192 96
Size: 409 154 477 997 1712 138
Fragments arranged by size:
2870 1712 1443 1231 997 477 409 365
274 154 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 10194 10226 2225
Size: 32 7937 32 2233
Fragments arranged by size:
7937 2233 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5275 7188 1316
Size: 3959 1913 4362
Fragments arranged by size:
4362 3959 1913
XbaI T'CTAG_A
Cuts at: 7570 7570
Size: 10234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6400 6400
Size: 10234
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10234
XmaI C'CCGG_G
Cuts at: 1870 7559 1870
Size: 5689 4545
XmnI GAAnn'nnTTC
Cuts at: 2436 3438 5499 6294 8363 2436
Size: 1002 2061 795 2069 4307
Fragments arranged by size:
4307 2069 2061 1002 795
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6107 8042 276
Size: 53 83 186 936 4573 1935 2468
Fragments arranged by size:
4573 2468 1935 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SfoI SrfI SwaI