Restriction map for
pFN21AE3483
(Circular) (Six-base) MAPSORT of: pFN21AE3483.seq Check: 6776 from: 1 to: 9943
pFN21AE3483 9943 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6008 6469 6008
Size: 461 9482
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7753 278
Size: 53 83 186 936 6217 2468
Fragments arranged by size:
6217 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7286 1937
Size: 5349 4594
Acc65I G'GTAC_C
Cuts at: 7264 7264
Size: 9943
AclI AA'CG_TT
Cuts at: 1563 8070 8443 1563
Size: 6507 373 3063
Fragments arranged by size:
6507 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2475 2812 4512 4758 5211 5968 6213 6288
Size: 843 337 1700 246 453 757 245 75
Cuts at: 6288 6339 6869 8006 9390 9658 1632
Size: 51 530 1137 1384 268 1917
Fragments arranged by size:
1917 1700 1384 1137 843 757 530 453
337 268 246 245 75 51
AfeI AGC'GCT
Cuts at: 4775 4775
Size: 9943
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9746
AflIII A'CryG_T
Cuts at: 1203 2032 8842 9819 1203
Size: 829 6810 977 1327
Fragments arranged by size:
6810 1327 977 829
AgeI A'CCGG_T
Cuts at: 8774 8774
Size: 9943
AhdI GACnn_n'nnGTC
Cuts at: 5641 5974 8672 5641
Size: 333 2698 6912
Fragments arranged by size:
6912 2698 333
AleI CACnn'nnGTG
Cuts at: 1166 2651 1166
Size: 1485 8458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9943
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3663 3695 3663
Size: 32 9911
AlwNI CAG_nnn'CTG
Cuts at: 2102 4412 4718 6145 6925 7000 7686 9258 2102
Size: 2310 306 1427 780 75 686 1572 2787
Fragments arranged by size:
2787 2310 1572 1427 780 686 306 75
ApaI G_GGCC'C
Cuts at: 3479 5784 7013 3479
Size: 2305 1229 6409
Fragments arranged by size:
6409 2305 1229
ApaLI G'TGCA_C
Cuts at: 8000 9156 8000
Size: 1156 8787
ApoI r'AATT_y
Cuts at: 1493 2971 3785 7227 7252 7478 7630 9739 1493
Size: 1478 814 3442 25 226 152 2109 1697
Fragments arranged by size:
3442 2109 1697 1478 814 226 152 25
AseI AT'TA_AT
Cuts at: 160 1032 8497 160
Size: 872 7465 1606
Fragments arranged by size:
7465 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9943
AvaI C'yCGr_G
Cuts at: 1870 1943 3470 5178 6707 6909 7268 1870
Size: 73 1527 1708 1529 202 359 4545
Fragments arranged by size:
4545 1708 1529 1527 359 202 73
BamHI G'GATC_C
Cuts at: 5182 6223 6963 7273 7640 5182
Size: 1041 740 310 367 7485
Fragments arranged by size:
7485 1041 740 367 310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2110 7264 8719 618
Size: 325 217 627 323 5154 1455 1842
Fragments arranged by size:
5154 1842 1455 627 325 323 217
BanII G_rGCy'C
Cuts at: 1391 3159 3479 4106 5784 6627 7013 7262 1391
Size: 1768 320 627 1678 843 386 249 4072
Fragments arranged by size:
4072 1768 1678 843 627 386 320 249
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4052 4149 4960 5286 5928 6279 928
Size: 971 2153 97 811 326 642 351 4592
Fragments arranged by size:
4592 2153 971 811 642 351 326 97
BbvCI CC'TCA_GC
Cuts at: 5692 5692
Size: 9943
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8134 8168 1903
Size: 34 6197 34 3678
Fragments arranged by size:
6197 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2476 7835 9045 2476
Size: 5359 1210 3374
Fragments arranged by size:
5359 3374 1210
BclI T'GATC_A
Cuts at: 1548 5481 7113 1548
Size: 3933 1632 4378
Fragments arranged by size:
4378 3933 1632
BfrBI ATG'CAT
Cuts at: 3905 5072 3905
Size: 1167 8776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4450 4872 8554
Size: 107 122 71 631 605 2778 422 3682
Cuts at: 8554 136
Size: 1525
Fragments arranged by size:
3682 2778 1525 631 605 422 122 107
71
BglII A'GATC_T
Cuts at: 6081 7021 9938 6081
Size: 940 2917 6086
Fragments arranged by size:
6086 2917 940
BlpI GC'TnA_GC
Cuts at: 2270 7360 2270
Size: 5090 4853
Bme1580I G_kGCm'C
Cuts at: 1790 3479 3568 5784 7013 8004 9160 1790
Size: 1689 89 2216 1229 991 1156 2573
Fragments arranged by size:
2573 2216 1689 1229 1156 991 89
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9943
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5593 6754 7674
Size: 416 53 83 399 24 4176 1161 920
Cuts at: 7674 8632 442
Size: 958 1753
Fragments arranged by size:
4176 1753 1161 958 920 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9943
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4233 5524 4233
Size: 1291 8652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2503 3510 4329 4389 4887 5752 6977 8603
Size: 1264 1007 819 60 498 865 1225 1626
Cuts at: 8603 1239
Size: 2579
Fragments arranged by size:
2579 1626 1264 1225 1007 865 819 498
60
Bpu10I CC'TnA_GC
Cuts at: 2394 4770 5692 2394
Size: 2376 922 6645
Fragments arranged by size:
6645 2376 922
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2356 4203 4977 5864 6536 7181 8068 8933 9231
Size: 1847 774 887 672 645 887 865 298
Cuts at: 9231 2356
Size: 3068
Fragments arranged by size:
3068 1847 887 887 865 774 672 645
298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2986 3230 5674 8606 882
Size: 617 1487 244 2444 2932 2219
Fragments arranged by size:
2932 2444 2219 1487 617 244
BsaAI yAC'GTr
Cuts at: 493 1204 7734 493
Size: 711 6530 2702
Fragments arranged by size:
6530 2702 711
BsaBI GATnn'nnATC
Cuts at: 9937 9937
Size: 9943
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6276 6978 7750 8132
Size: 53 83 186 936 4743 702 772 382
Cuts at: 8132 8767 275
Size: 635 1451
Fragments arranged by size:
4743 1451 936 772 702 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 8376 8774 9048 9195 9606 8376
Size: 398 274 147 411 8713
Fragments arranged by size:
8713 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2830 2860 3725 3755 4549
Size: 30 165 30 1427 30 865 30 794
Cuts at: 4549 4579 4621 4651 1178
Size: 30 42 30 6470
Fragments arranged by size:
6470 1427 865 794 165 42 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2720 2756 2759 4332 4835 4887 4890 5113
Size: 1534 36 3 1573 503 52 3 223
Cuts at: 5113 5166 6385 1186
Size: 53 1219 4744
Fragments arranged by size:
4744 1573 1534 1219 503 223 53 52
36 3 3
BseYI C'CCAG_C
Cuts at: 3335 4195 5936 6444 6626 9146 9642 3335
Size: 860 1741 508 182 2520 496 3636
Fragments arranged by size:
3636 2520 1741 860 508 496 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5206 6797 1163
Size: 573 3470 1591 4309
Fragments arranged by size:
4309 3470 1591 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7393 8154 9182 1540
Size: 275 186 5392 761 1028 2301
Fragments arranged by size:
5392 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2648 2751 3468 4430 6522 7180 8004 8089 9160
Size: 103 717 962 2092 658 824 85 1071
Cuts at: 9160 2648
Size: 3431
Fragments arranged by size:
3431 2092 1071 962 824 717 658 103
85
BsmI GAATG_Cn'
Cuts at: 2057 3000 5074 7454 7547 2057
Size: 943 2074 2380 93 4453
Fragments arranged by size:
4453 2380 2074 943 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2648 2751 3159 3468 3479 3568 4106
Size: 399 858 103 408 309 11 89 538
Cuts at: 4106 4430 5784 6522 6627 7013 7180 7262 8004
Size: 324 1354 738 105 386 167 82 742
Cuts at: 8004 8089 9160 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1354 1071 858 742 738 538 408
399 386 324 309 167 105 103 89
85 82 11
BspEI T'CCGG_A
Cuts at: 9606 9606
Size: 9943
BspHI T'CATG_A
Cuts at: 3835 6354 7110 7832 9562 3835
Size: 2519 756 722 1730 4216
Fragments arranged by size:
4216 2519 1730 756 722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4640 6008 6469 6576 6885 7298 844
Size: 3796 1368 461 107 309 413 3489
Fragments arranged by size:
3796 3489 1368 461 413 309 107
BsrBI CCG'CTC
Cuts at: 1394 1823 6897 6961 7830 1394
Size: 429 5074 64 869 3507
Fragments arranged by size:
5074 3507 869 429 64
BsrDI GCAATG_nn'
Cuts at: 65 1248 2134 8438 8612 65
Size: 1183 886 6304 174 1396
Fragments arranged by size:
6304 1396 1183 886 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2673 5772 8587 8774 1700
Size: 110 863 3099 2815 187 2869
Fragments arranged by size:
3099 2869 2815 863 187 110
BsrGI T'GTAC_A
Cuts at: 96 3292 4974 96
Size: 3196 1682 5065
Fragments arranged by size:
5065 3196 1682
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9943
BssSI C'ACGA_G
Cuts at: 2750 6867 8003 9015 2750
Size: 4117 1136 1012 3678
Fragments arranged by size:
4117 3678 1136 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2061 3254 4760 5069 5220 5441 6023 794
Size: 1267 1193 1506 309 151 221 582 4714
Fragments arranged by size:
4714 1506 1267 1193 582 309 221 151
BstBI TT'CG_AA
Cuts at: 8747 8747
Size: 9943
BstEII G'GTnAC_C
Cuts at: 1184 3119 4652 1184
Size: 1935 1533 6475
Fragments arranged by size:
6475 1935 1533
BstXI CCAn_nnnn'nTGG
Cuts at: 5755 6611 5755
Size: 856 9087
BstYI r'GATC_y
Cuts at: 1972 4203 5182 5290 6055 6081 6223 6711 6963
Size: 2231 979 108 765 26 142 488 252
Cuts at: 6963 7021 7273 7640 8025 8042 9494 9580 9592
Size: 58 252 367 385 17 1452 86 12
Cuts at: 9592 9938 1972
Size: 346 1977
Fragments arranged by size:
2231 1977 1452 979 765 488 385 367
346 252 252 142 108 86 58 26
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2122 2787 3453 5529 513
Size: 552 1057 665 666 2076 4927
Fragments arranged by size:
4927 2076 1057 666 665 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5389 487
Size: 683 56 4163 5041
Fragments arranged by size:
5041 4163 683 56
BtsI GCAGTG_nn'
Cuts at: 4384 5465 5602 6544 7103 7459 8253 8273 4384
Size: 1081 137 942 559 356 794 20 6054
Fragments arranged by size:
6054 1081 942 794 559 356 137 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9943
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9908
DraI TTT'AAA
Cuts at: 2068 4789 7247 2068
Size: 2721 2458 4764
Fragments arranged by size:
4764 2721 2458
DraIII CAC_nnn'GTG
Cuts at: 6382 6382
Size: 9943
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8950 809
Size: 507 7634 1802
Fragments arranged by size:
7634 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2784 3456 4230
Size: 54 1425 50 275 18 954 672 774
Cuts at: 4230 6927 7390 8279 9908 8
Size: 2697 463 889 1629 43
Fragments arranged by size:
2697 1629 1425 954 889 774 672 463
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7390 1537
Size: 275 5578 4090
Fragments arranged by size:
5578 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2709 2769 3264 3366 4380 5090 6255 6683
Size: 1356 60 495 102 1014 710 1165 428
Cuts at: 6683 7031 7873 1353
Size: 348 842 3423
Fragments arranged by size:
3423 1356 1165 1014 842 710 495 428
348 102 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4790 8530 8904 9050 387
Size: 1064 138 3201 3740 374 146 1280
Fragments arranged by size:
3740 3201 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2475 2503 2812 3510 4329 4389 4512
Size: 393 843 28 309 698 819 60 123
Cuts at: 4512 4758 4887 5211 5752 5968 6213 6288 6339
Size: 246 129 324 541 216 245 75 51
Cuts at: 6339 6869 6977 8006 8603 9390 9658 1239
Size: 530 108 1029 597 787 268 1524
Fragments arranged by size:
1524 1029 843 819 787 698 597 541
530 393 324 309 268 246 245 216
129 123 108 75 60 51 28
EcoNI CCTnn'n_nnAGG
Cuts at: 4992 5659 4992
Size: 667 9276
EcoO109I rG'GnC_Cy
Cuts at: 3475 3476 5515 5781 7009 7010 3475
Size: 1 2039 266 1228 1 6408
Fragments arranged by size:
6408 2039 1228 266 1 1
EcoRI G'AATT_C
Cuts at: 7252 7630 7252
Size: 378 9565
EcoRV GAT'ATC
Cuts at: 4782 5572 4782
Size: 790 9153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 3269 4011 6077 2080
Size: 1189 742 2066 5946
Fragments arranged by size:
5946 2066 1189 742
FspI TGC'GCA
Cuts at: 5672 8449 5672
Size: 2777 7166
HaeII r_GCGC'y
Cuts at: 1678 4777 4915 9090 1678
Size: 3099 138 4175 2531
Fragments arranged by size:
4175 3099 2531 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3014 3046 5125 5157 5627
Size: 32 135 32 1442 32 2079 32 470
Cuts at: 5627 5659 5960 5992 8584 8616 8658 8690 1373
Size: 32 301 32 2592 32 42 32 2626
Fragments arranged by size:
2626 2592 2079 1442 470 301 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3484 4710 7287 7533 669
Size: 988 281 1546 1226 2577 246 3079
Fragments arranged by size:
3079 2577 1546 1226 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2576 4371 4792 748
Size: 1828 1795 421 5899
Fragments arranged by size:
5899 1828 1795 421
HpaI GTT'AAC
Cuts at: 3484 7533 3484
Size: 4049 5894
KpnI G_GTAC'C
Cuts at: 7268 7268
Size: 9943
MfeI C'AATT_G
Cuts at: 7542 7542
Size: 9943
MluI A'CGCG_T
Cuts at: 9819 9819
Size: 9943
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4351 4787 5772 9057 9241 1305
Size: 3046 436 985 3285 184 2007
Fragments arranged by size:
3285 3046 2007 985 436 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2786 3458 4232 6929 10
Size: 54 1425 343 954 672 774 2697 3024
Fragments arranged by size:
3024 2697 1425 954 774 672 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2127 2651 3996 4886 5889 7901 8260
Size: 648 961 524 1345 890 1003 2012 359
Cuts at: 8260 8419 518
Size: 159 2042
Fragments arranged by size:
2042 2012 1345 1003 961 890 648 524
359 159
MspA1I CmG'CkG
Cuts at: 1606 4757 5973 6925 6933 7359 8036 9184 9429
Size: 3151 1216 952 8 426 677 1148 245
Cuts at: 9429 1606
Size: 2120
Fragments arranged by size:
3151 2120 1216 1148 952 677 426 245
8
NaeI GCC'GGC
Cuts at: 5774 5774
Size: 9943
NcoI C'CATG_G
Cuts at: 513 1065 2122 2787 3453 5529 513
Size: 552 1057 665 666 2076 4927
Fragments arranged by size:
4927 2076 1057 666 665 552
NdeI CA'TA_TG
Cuts at: 387 3907 6046 387
Size: 3520 2139 4284
Fragments arranged by size:
4284 3520 2139
NgoMIV G'CCGG_C
Cuts at: 5772 5772
Size: 9943
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9943
NotI GC'GGCC_GC
Cuts at: 7390 7390
Size: 9943
NsiI A_TGCA'T
Cuts at: 3907 5074 3907
Size: 1167 8776
NspI r_CATG'y
Cuts at: 1132 1229 2036 2630 4891 7301 8846 1132
Size: 97 807 594 2261 2410 1545 2229
Fragments arranged by size:
2410 2261 2229 1545 807 594 97
PasI CC'CwG_GG
Cuts at: 1348 6216 1348
Size: 4868 5075
PciI A'CATG_T
Cuts at: 2032 8842 2032
Size: 6810 3133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6382 6700 1108
Size: 5274 318 4351
Fragments arranged by size:
5274 4351 318
PfoI T'CCnGG_A
Cuts at: 6717 6717
Size: 9943
PmeI GTTT'AAAC
Cuts at: 7247 7247
Size: 9943
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8012 8044 9525 9557 8012
Size: 32 1481 32 8398
Fragments arranged by size:
8398 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5515 5515
Size: 9943
PshAI GACnn'nnGTC
Cuts at: 1613 6177 1613
Size: 4564 5379
PsiI TTA'TAA
Cuts at: 3798 7513 3798
Size: 3715 6228
PspOMI G'GGCC_C
Cuts at: 3475 5780 7009 3475
Size: 2305 1229 6409
Fragments arranged by size:
6409 2305 1229
PspXI vC'TCGA_Gb
Cuts at: 3470 3470
Size: 9943
PstI C_TGCA'G
Cuts at: 830 2910 6622 6921 7236 7295 830
Size: 2080 3712 299 315 59 3478
Fragments arranged by size:
3712 3478 2080 315 299 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9943
PvuII CAG'CTG
Cuts at: 4757 5973 6925 6933 4757
Size: 1216 952 8 7767
Fragments arranged by size:
7767 1216 952 8
SalI G'TCGA_C
Cuts at: 1936 7285 1936
Size: 5349 4594
SapI GCTCTTCn'nnn_
Cuts at: 2769 3366 4380 6683 2769
Size: 597 1014 2303 6029
Fragments arranged by size:
6029 2303 1014 597
SbfI CC_TGCA'GG
Cuts at: 7295 7295
Size: 9943
ScaI AGT'ACT
Cuts at: 8191 9903 8191
Size: 1712 8231
SexAI A'CCwGG_T
Cuts at: 2044 4703 2044
Size: 2659 7284
SfcI C'TryA_G
Cuts at: 826 1045 2214 2906 3324 4174 5078 6618 6917
Size: 219 1169 692 418 850 904 1540 299
Cuts at: 6917 7200 7232 7291 8426 9107 9298 826
Size: 283 32 59 1135 681 191 1471
Fragments arranged by size:
1540 1471 1169 1135 904 850 692 681
418 299 283 219 191 59 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9943
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9943
SmaI CCC'GGG
Cuts at: 1872 6911 7270 1872
Size: 5039 359 4545
Fragments arranged by size:
5039 4545 359
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2335 3470 4218 4992 5843 6515
Size: 197 926 392 1135 748 774 851 672
Cuts at: 6515 7160 8047 8948 9210 820
Size: 645 887 901 262 1553
Fragments arranged by size:
1553 1135 926 901 887 851 774 748
672 645 392 262 197
SnaBI TAC'GTA
Cuts at: 493 7734 493
Size: 7241 2702
SpeI A'CTAG_T
Cuts at: 152 3659 152
Size: 3507 6436
SphI G_CATG'C
Cuts at: 1132 7301 1132
Size: 6169 3774
SspI AAT'ATT
Cuts at: 5 52 7867 5
Size: 47 7815 2081
Fragments arranged by size:
7815 2081 47
StuI AGG'CCT
Cuts at: 1517 5665 1517
Size: 4148 5795
StyI C'CwwG_G
Cuts at: 513 1065 2122 2787 3453 3686 5382 5511 5529
Size: 552 1057 665 666 233 1696 129 18
Cuts at: 5529 5655 6604 7382 513
Size: 126 949 778 3074
Fragments arranged by size:
3074 1696 1057 949 778 666 665 552
233 129 126 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2128 2135 3736 5452 7970 7987 8140
Size: 1255 548 7 1601 1716 2518 17 153
Cuts at: 8140 8325 8770 325
Size: 185 445 1498
Fragments arranged by size:
2518 1716 1601 1498 1255 548 445 185
153 17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2722 3292 4528
Size: 274 80 33 51 1443 745 570 1236
Cuts at: 4528 4974 7057 8189 9901 96
Size: 446 2083 1132 1712 138
Fragments arranged by size:
2083 1712 1443 1236 1132 745 570 446
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2032 2064 2830 2862 4498 4530 4549 4581 9903
Size: 32 766 32 1636 32 19 32 5322
Cuts at: 9903 9935 2032
Size: 32 2040
Fragments arranged by size:
5322 2040 1636 766 32 32 32 32
32 19
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9943
XbaI T'CTAG_A
Cuts at: 5342 7279 5342
Size: 1937 8006
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2236 5389 5957 6587 2236
Size: 3153 568 630 5592
Fragments arranged by size:
5592 3153 630 568
XhoI C'TCGA_G
Cuts at: 1943 3470 1943
Size: 1527 8416
XmaI C'CCGG_G
Cuts at: 1870 6909 7268 1870
Size: 5039 359 4545
Fragments arranged by size:
5039 4545 359
XmnI GAAnn'nnTTC
Cuts at: 8072 8072
Size: 9943
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7751 276
Size: 53 83 186 936 6217 2468
Fragments arranged by size:
6217 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I
EcoICRI FseI FspAI KasI NarI NruI PacI PmlI
PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI