Restriction map for
pFN21AE3478
(Circular) (Six-base) MAPSORT of: pFN21AE3478.seq Check: 773 from: 1 to: 9544
pFN21AE3478 9544 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7354 278
Size: 53 83 186 936 5818 2468
Fragments arranged by size:
5818 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2762 4140 6174 6887 1937
Size: 825 1378 2034 713 4594
Fragments arranged by size:
4594 2034 1378 825 713
Acc65I G'GTAC_C
Cuts at: 6865 6865
Size: 9544
AclI AA'CG_TT
Cuts at: 1563 7671 8044 1563
Size: 6108 373 3063
Fragments arranged by size:
6108 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2809 4801 5216 6114 7607 8991 9259 1632
Size: 1177 1992 415 898 1493 1384 268 1917
Fragments arranged by size:
1992 1917 1493 1384 1177 898 415 268
AflII C'TTAA_G
Cuts at: 820 1017 5305 820
Size: 197 4288 5059
Fragments arranged by size:
5059 4288 197
AflIII A'CryG_T
Cuts at: 1203 3381 4096 5747 8443 9420 1203
Size: 2178 715 1651 2696 977 1327
Fragments arranged by size:
2696 2178 1651 1327 977 715
AgeI A'CCGG_T
Cuts at: 8375 8375
Size: 9544
AhdI GACnn_n'nnGTC
Cuts at: 8273 8273
Size: 9544
AleI CACnn'nnGTG
Cuts at: 1166 5622 1166
Size: 4456 5088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2862 5640 1184
Size: 1678 2778 5088
Fragments arranged by size:
5088 2778 1678
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5271 5303 5271
Size: 32 9512
AlwNI CAG_nnn'CTG
Cuts at: 2043 2345 3269 5038 6263 6701 7287 8859 2043
Size: 302 924 1769 1225 438 586 1572 2728
Fragments arranged by size:
2728 1769 1572 1225 924 586 438 302
ApaLI G'TGCA_C
Cuts at: 4086 7601 8757 4086
Size: 3515 1156 4873
Fragments arranged by size:
4873 3515 1156
ApoI r'AATT_y
Cuts at: 1493 2240 2544 3405 3597 3936 4029 4106 6506
Size: 747 304 861 192 339 93 77 2400
Cuts at: 6506 6853 7079 7231 9340 1493
Size: 347 226 152 2109 1697
Fragments arranged by size:
2400 2109 1697 861 747 347 339 304
226 192 152 93 77
AseI AT'TA_AT
Cuts at: 160 1032 5118 8098 160
Size: 872 4086 2980 1606
Fragments arranged by size:
4086 2980 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9544
AvaI C'yCGr_G
Cuts at: 1870 1943 3348 5181 6324 6869 1870
Size: 73 1405 1833 1143 545 4545
Fragments arranged by size:
4545 1833 1405 1143 545 73
BamHI G'GATC_C
Cuts at: 6874 7241 6874
Size: 367 9177
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2981 5912 6865 8320 618
Size: 325 217 627 1194 2931 953 1455 1842
Fragments arranged by size:
2931 1842 1455 1194 953 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4261 4473 4868 5021 6394 6863 1391
Size: 2870 212 395 153 1373 469 4072
Fragments arranged by size:
4072 2870 1373 469 395 212 153
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3081 3597 3884 5430 5841 928
Size: 971 1182 516 287 1546 411 4631
Fragments arranged by size:
4631 1546 1182 971 516 411 287
BbvCI CC'TCA_GC
Cuts at: 3783 5060 5757 3783
Size: 1277 697 7570
Fragments arranged by size:
7570 1277 697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4792 4826 7735 7769 1903
Size: 34 2855 34 2909 34 3678
Fragments arranged by size:
3678 2909 2855 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3031 5804 7436 8646 3031
Size: 2773 1632 1210 3929
Fragments arranged by size:
3929 2773 1632 1210
BclI T'GATC_A
Cuts at: 1548 3705 5989 6040 6339 1548
Size: 2157 2284 51 299 4753
Fragments arranged by size:
4753 2284 2157 299 51
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3852 8155
Size: 107 122 71 631 605 337 1843 4303
Cuts at: 8155 136
Size: 1525
Fragments arranged by size:
4303 1843 1525 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 3318 5368 9539 3318
Size: 2050 4171 3323
Fragments arranged by size:
4171 3323 2050
BlpI GC'TnA_GC
Cuts at: 6961 6961
Size: 9544
Bme1580I G_kGCm'C
Cuts at: 1790 4090 4701 5917 6708 7605 8761 1790
Size: 2300 611 1216 791 897 1156 2573
Fragments arranged by size:
2573 2300 1216 1156 897 791 611
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9544
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2482 2949 3247
Size: 416 53 83 399 24 1065 467 298
Cuts at: 3247 3406 3529 3579 4174 5872 7275 8233 442
Size: 159 123 50 595 1698 1403 958 1753
Fragments arranged by size:
1753 1698 1403 1065 958 595 467 416
399 298 159 123 83 53 50 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9544
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6813 6813
Size: 9544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2224 2749 2889 2975 4573 8204 1239
Size: 985 525 140 86 1598 3631 2579
Fragments arranged by size:
3631 2579 1598 985 525 140 86
Bpu10I CC'TnA_GC
Cuts at: 3783 5060 5628 5757 3783
Size: 1277 568 129 7570
Fragments arranged by size:
7570 1277 568 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3040 4037 7669 8534 8832 3040
Size: 997 3632 865 298 3752
Fragments arranged by size:
3752 3632 997 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5247 8207 882
Size: 617 3748 2960 2219
Fragments arranged by size:
3748 2960 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6206 7335 493
Size: 711 5002 1129 2702
Fragments arranged by size:
5002 2702 1129 711
BsaBI GATnn'nnATC
Cuts at: 3105 4637 5367 6084 9538 3105
Size: 1532 730 717 3454 3111
Fragments arranged by size:
3454 3111 1532 730 717
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7351 7733 8368 275
Size: 53 83 186 936 5818 382 635 1451
Fragments arranged by size:
5818 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5409 7977 8375 8649 8796 9207 5409
Size: 2568 398 274 147 411 5746
Fragments arranged by size:
5746 2568 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5737 5767 1178
Size: 30 165 30 4334 30 4955
Fragments arranged by size:
4955 4334 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 5745 6761 6802 6829 1186
Size: 849 3710 1016 41 27 3901
Fragments arranged by size:
3901 3710 1016 849 41 27
BseYI C'CCAG_C
Cuts at: 5341 5562 6475 8747 9243 5341
Size: 221 913 2272 496 5642
Fragments arranged by size:
5642 2272 913 496 221
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2282 3711 1163
Size: 573 546 1429 6996
Fragments arranged by size:
6996 1429 573 546
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6994 7755 8783 1540
Size: 275 186 4993 761 1028 2301
Fragments arranged by size:
4993 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2052 2735 4090 4261 4798 5021 5629 7605 7690
Size: 683 1355 171 537 223 608 1976 85
Cuts at: 7690 8761 2052
Size: 1071 2835
Fragments arranged by size:
2835 1976 1355 1071 683 608 537 223
171 85
BsmI GAATG_Cn'
Cuts at: 2134 7055 7148 2134
Size: 4921 93 4530
Fragments arranged by size:
4921 4530 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3016 3568 3016
Size: 552 8992
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2735 4090 4261 4473 4701 4798
Size: 399 262 683 1355 171 212 228 97
Cuts at: 4798 4868 5021 5629 5917 6394 6708 6863 7605
Size: 70 153 608 288 477 314 155 742
Cuts at: 7605 7690 8761 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1355 1071 742 683 608 477 399
314 288 262 228 212 171 155 153
97 85 70
BspEI T'CCGG_A
Cuts at: 9207 9207
Size: 9544
BspHI T'CATG_A
Cuts at: 7433 9163 7433
Size: 1730 7814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2978 3890 6899 844
Size: 2134 912 3009 3489
Fragments arranged by size:
3489 3009 2134 912
BsrBI CCG'CTC
Cuts at: 1394 1823 2060 7431 1394
Size: 429 237 5371 3507
Fragments arranged by size:
5371 3507 429 237
BsrDI GCAATG_nn'
Cuts at: 65 1248 3090 4341 4699 5932 8039 8213 65
Size: 1183 1842 1251 358 1233 2107 174 1396
Fragments arranged by size:
2107 1842 1396 1251 1233 1183 358 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6570 8188 8375 1700
Size: 110 4760 1618 187 2869
Fragments arranged by size:
4760 2869 1618 187 110
BsrGI T'GTAC_A
Cuts at: 96 6830 96
Size: 6734 2810
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9544
BssSI C'ACGA_G
Cuts at: 7604 8616 7604
Size: 1012 8532
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9544
BstBI TT'CG_AA
Cuts at: 8348 8348
Size: 9544
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9544
BstXI CCAn_nnnn'nTGG
Cuts at: 3221 5704 3221
Size: 2483 7061
BstYI r'GATC_y
Cuts at: 1972 2381 2440 3176 3318 3364 5368 6305 6874
Size: 409 59 736 142 46 2004 937 569
Cuts at: 6874 7241 7626 7643 9095 9181 9193 9539 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2004 1977 1452 937 736 569 409 385
367 346 142 86 59 46 17 12
BstZ17I GTA'TAC
Cuts at: 2763 6175 2763
Size: 3412 6132
Bsu36I CC'TnA_GG
Cuts at: 2018 3825 2018
Size: 1807 7737
BtgI C'CryG_G
Cuts at: 513 1065 2004 2808 3369 513
Size: 552 939 804 561 6688
Fragments arranged by size:
6688 939 804 561 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5829 487
Size: 683 56 4603 4202
Fragments arranged by size:
4603 4202 683 56
BtsI GCAGTG_nn'
Cuts at: 2664 3198 4090 4699 5395 5906 6064 6376 7060
Size: 534 892 609 696 511 158 312 684
Cuts at: 7060 7854 7874 2664
Size: 794 20 4334
Fragments arranged by size:
4334 892 794 696 684 609 534 511
312 158 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9544
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4570 4605 529
Size: 35 4006 35 5468
Fragments arranged by size:
5468 4006 35 35
DraI TTT'AAA
Cuts at: 6848 6848
Size: 9544
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4643 8551 809
Size: 507 3327 3908 1802
Fragments arranged by size:
3908 3327 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2552 3476 4591
Size: 54 1425 50 275 18 722 924 1115
Cuts at: 4591 6991 7880 9509 8
Size: 2400 889 1629 43
Fragments arranged by size:
2400 1629 1425 1115 924 889 722 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6991 1537
Size: 275 5179 4090
Fragments arranged by size:
5179 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4820 6315 7474 1353
Size: 3467 1495 1159 3423
Fragments arranged by size:
3467 3423 1495 1159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8131 8505 8651 387
Size: 1064 138 6542 374 146 1280
Fragments arranged by size:
6542 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4259 5019 4259
Size: 760 8784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2224 2749 2809 2889 2975 4573 4801
Size: 393 592 525 60 80 86 1598 228
Cuts at: 4801 5216 6114 7607 8204 8991 9259 1239
Size: 415 898 1493 597 787 268 1524
Fragments arranged by size:
1598 1524 1493 898 787 597 592 525
415 393 268 228 86 80 60
EcoNI CCTnn'n_nnAGG
Cuts at: 3114 4278 3114
Size: 1164 8380
EcoO109I rG'GnC_Cy
Cuts at: 3829 5287 3829
Size: 1458 8086
EcoRI G'AATT_C
Cuts at: 4106 6853 7231 4106
Size: 2747 378 6419
Fragments arranged by size:
6419 2747 378
EcoRV GAT'ATC
Cuts at: 3928 3928
Size: 9544
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2690 5706 6438 2690
Size: 3016 732 5796
Fragments arranged by size:
5796 3016 732
FspI TGC'GCA
Cuts at: 8050 8050
Size: 9544
HaeII r_GCGC'y
Cuts at: 1678 8691 1678
Size: 7013 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2786 2818 3697 3729 3912
Size: 32 135 32 1214 32 879 32 183
Cuts at: 3912 3944 5126 5158 5711 5743 6184 6216 8185
Size: 32 1182 32 553 32 441 32 1969
Cuts at: 8185 8217 8259 8291 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1969 1214 1182 879 553 441 183
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3460 6888 7134 669
Size: 988 281 1522 3428 246 3079
Fragments arranged by size:
3428 3079 1522 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2588 3993 748
Size: 1840 1405 6299
Fragments arranged by size:
6299 1840 1405
HpaI GTT'AAC
Cuts at: 3460 7134 3460
Size: 3674 5870
KpnI G_GTAC'C
Cuts at: 6869 6869
Size: 9544
MfeI C'AATT_G
Cuts at: 5316 5854 7143 5316
Size: 538 1289 7717
Fragments arranged by size:
7717 1289 538
MluI A'CGCG_T
Cuts at: 9420 9420
Size: 9544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2125 2394 3795 4185 4248 5128 5768 6113
Size: 820 269 1401 390 63 880 640 345
Cuts at: 6113 6617 8658 8842 1305
Size: 504 2041 184 2007
Fragments arranged by size:
2041 2007 1401 880 820 640 504 390
345 269 184 63
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2554 3478 4593 10
Size: 54 1425 343 722 924 1115 4961
Fragments arranged by size:
4961 1425 1115 924 722 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3219 5120 5622 5844 6158 7502 7861
Size: 648 2053 1901 502 222 314 1344 359
Cuts at: 7861 8020 518
Size: 159 2042
Fragments arranged by size:
2053 2042 1901 1344 648 502 359 314
222 159
MspA1I CmG'CkG
Cuts at: 1606 2165 3718 6000 6960 7637 8785 9030 1606
Size: 559 1553 2282 960 677 1148 245 2120
Fragments arranged by size:
2282 2120 1553 1148 960 677 559 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2808 3369 513
Size: 552 939 804 561 6688
Fragments arranged by size:
6688 939 804 561 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9544
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9544
NotI GC'GGCC_GC
Cuts at: 6991 6991
Size: 9544
NspI r_CATG'y
Cuts at: 1132 1229 2310 2822 3385 4100 5751 6902 8447
Size: 97 1081 512 563 715 1651 1151 1545
Cuts at: 8447 1132
Size: 2229
Fragments arranged by size:
2229 1651 1545 1151 1081 715 563 512
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9544
PciI A'CATG_T
Cuts at: 3381 4096 5747 8443 3381
Size: 715 1651 2696 4482
Fragments arranged by size:
4482 2696 1651 715
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3980 1108
Size: 2872 6672
PfoI T'CCnGG_A
Cuts at: 2952 6313 2952
Size: 3361 6183
PmeI GTTT'AAAC
Cuts at: 6848 6848
Size: 9544
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5864 5896 7613 7645 9126 9158 5864
Size: 32 1717 32 1481 32 6250
Fragments arranged by size:
6250 1717 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5287 5287
Size: 9544
PshAI GACnn'nnGTC
Cuts at: 1613 3081 1613
Size: 1468 8076
PsiI TTA'TAA
Cuts at: 2531 7114 2531
Size: 4583 4961
PspXI vC'TCGA_Gb
Cuts at: 5181 5181
Size: 9544
PstI C_TGCA'G
Cuts at: 830 2039 3069 6602 6896 830
Size: 1209 1030 3533 294 3478
Fragments arranged by size:
3533 3478 1209 1030 294
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9544
PvuII CAG'CTG
Cuts at: 2165 3718 2165
Size: 1553 7991
SacI G_AGCT'C
Cuts at: 4261 5021 4261
Size: 760 8784
SalI G'TCGA_C
Cuts at: 1936 6886 1936
Size: 4950 4594
SapI GCTCTTCn'nnn_
Cuts at: 4820 4820
Size: 9544
SbfI CC_TGCA'GG
Cuts at: 6896 6896
Size: 9544
ScaI AGT'ACT
Cuts at: 3582 7792 9504 3582
Size: 4210 1712 3622
Fragments arranged by size:
4210 3622 1712
SexAI A'CCwGG_T
Cuts at: 5703 5703
Size: 9544
SfcI C'TryA_G
Cuts at: 826 1045 2035 3065 3817 6553 6598 6892 8027
Size: 219 990 1030 752 2736 45 294 1135
Cuts at: 8027 8708 8899 826
Size: 681 191 1471
Fragments arranged by size:
2736 1471 1135 1030 990 752 681 294
219 191 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9544
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9544
SmaI CCC'GGG
Cuts at: 1872 3350 6871 1872
Size: 1478 3521 4545
Fragments arranged by size:
4545 3521 1478
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3019 4052 5181 5305 6324 7648
Size: 197 926 1076 1033 1129 124 1019 1324
Cuts at: 7648 8549 8811 820
Size: 901 262 1553
Fragments arranged by size:
1553 1324 1129 1076 1033 1019 926 901
262 197 124
SnaBI TAC'GTA
Cuts at: 493 7335 493
Size: 6842 2702
SpeI A'CTAG_T
Cuts at: 152 3583 152
Size: 3431 6113
SphI G_CATG'C
Cuts at: 1132 6902 1132
Size: 5770 3774
SspI AAT'ATT
Cuts at: 5 52 7468 5
Size: 47 7416 2081
Fragments arranged by size:
7416 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9544
StyI C'CwwG_G
Cuts at: 513 1065 2004 2808 3369 3492 6663 6983 513
Size: 552 939 804 561 123 3171 320 3074
Fragments arranged by size:
3171 3074 939 804 561 552 320 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3617 6303 6402 7571 7588 7741 7926
Size: 1255 2037 2686 99 1169 17 153 185
Cuts at: 7926 8371 325
Size: 445 1498
Fragments arranged by size:
2686 2037 1498 1255 1169 445 185 153
99 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2331 2460 2715
Size: 274 80 33 51 1443 354 129 255
Cuts at: 2715 3580 4161 6830 7790 9502 96
Size: 865 581 2669 960 1712 138
Fragments arranged by size:
2669 1712 1443 960 865 581 354 274
255 138 129 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4666 4698 4777 4809 5442 5474 9504 9536 4666
Size: 32 79 32 633 32 4030 32 4674
Fragments arranged by size:
4674 4030 633 79 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9544
XbaI T'CTAG_A
Cuts at: 6880 6880
Size: 9544
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4840 5891 4840
Size: 1051 8493
XhoI C'TCGA_G
Cuts at: 1943 5181 6324 1943
Size: 3238 1143 5163
Fragments arranged by size:
5163 3238 1143
XmaI C'CCGG_G
Cuts at: 1870 3348 6869 1870
Size: 1478 3521 4545
Fragments arranged by size:
4545 3521 1478
XmnI GAAnn'nnTTC
Cuts at: 3675 7673 3675
Size: 3998 5546
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7352 276
Size: 53 83 186 936 5818 2468
Fragments arranged by size:
5818 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI DraIII FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI