Restriction map for
pFN21AE3466
(Circular) (Six-base) MAPSORT of: pFN21AE3466.seq Check: 4256 from: 1 to: 8875
pFN21AE3466 8875 bp
With 182 enzymes: SgfI *
August 1, 2019 17:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6685 278
Size: 53 83 186 936 5149 2468
Fragments arranged by size:
5149 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2370 5007 6218 1937
Size: 433 2637 1211 4594
Fragments arranged by size:
4594 2637 1211 433
Acc65I G'GTAC_C
Cuts at: 6196 6196
Size: 8875
AclI AA'CG_TT
Cuts at: 1563 7002 7375 1563
Size: 5439 373 3063
Fragments arranged by size:
5439 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 2939 4195 4626 4692 6938 8322 8590
Size: 590 717 1256 431 66 2246 1384 268
Cuts at: 8590 1632
Size: 1917
Fragments arranged by size:
2246 1917 1384 1256 717 590 431 268
66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8678
AflIII A'CryG_T
Cuts at: 1203 2434 4091 5094 5688 7774 8751 1203
Size: 1231 1657 1003 594 2086 977 1327
Fragments arranged by size:
2086 1657 1327 1231 1003 977 594
AgeI A'CCGG_T
Cuts at: 2574 7706 2574
Size: 5132 3743
AhdI GACnn_n'nnGTC
Cuts at: 2467 7604 2467
Size: 5137 3738
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2057 1184
Size: 873 8002
AlwNI CAG_nnn'CTG
Cuts at: 2847 3062 6618 8190 2847
Size: 215 3556 1572 3532
Fragments arranged by size:
3556 3532 1572 215
ApaI G_GGCC'C
Cuts at: 2986 5223 2986
Size: 2237 6638
ApaLI G'TGCA_C
Cuts at: 3720 6932 8088 3720
Size: 3212 1156 4507
Fragments arranged by size:
4507 3212 1156
ApoI r'AATT_y
Cuts at: 1493 2608 3217 3436 3788 4060 4242 4306 4792
Size: 1115 609 219 352 272 182 64 486
Cuts at: 4792 5316 5503 5648 6184 6410 6562 8671 1493
Size: 524 187 145 536 226 152 2109 1697
Fragments arranged by size:
2109 1697 1115 609 536 524 486 352
272 226 219 187 182 152 145 64
AseI AT'TA_AT
Cuts at: 160 1032 7429 160
Size: 872 6397 1606
Fragments arranged by size:
6397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8875
AvaI C'yCGr_G
Cuts at: 1870 1943 4321 6200 1870
Size: 73 2378 1879 4545
Fragments arranged by size:
4545 2378 1879 73
BamHI G'GATC_C
Cuts at: 3970 4479 6205 6572 3970
Size: 509 1726 367 6273
Fragments arranged by size:
6273 1726 509 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2198 3624 5964 6196 7651
Size: 325 217 627 411 1426 2340 232 1455
Cuts at: 7651 618
Size: 1842
Fragments arranged by size:
2340 1842 1455 1426 627 411 325 232
217
BanII G_rGCy'C
Cuts at: 1391 2511 2986 5223 5848 6194 1391
Size: 1120 475 2237 625 346 4072
Fragments arranged by size:
4072 2237 1120 625 475 346
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2238 5552 5911 928
Size: 971 339 3314 359 3892
Fragments arranged by size:
3892 3314 971 359 339
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7066 7100 1903
Size: 34 5129 34 3678
Fragments arranged by size:
5129 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5091 6108 6767 7977 5091
Size: 1017 659 1210 5989
Fragments arranged by size:
5989 1210 1017 659
BclI T'GATC_A
Cuts at: 1548 2386 1548
Size: 838 8037
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 7486 136
Size: 107 122 71 631 605 337 5477 1525
Fragments arranged by size:
5477 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3681 4891 5494 5542 8870 3681
Size: 1210 603 48 3328 3686
Fragments arranged by size:
3686 3328 1210 603 48
BlpI GC'TnA_GC
Cuts at: 6292 6292
Size: 8875
Bme1580I G_kGCm'C
Cuts at: 1790 2986 3724 4963 5223 6936 8092 1790
Size: 1196 738 1239 260 1713 1156 2573
Fragments arranged by size:
2573 1713 1239 1196 1156 738 260
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2271 3531 5217
Size: 416 53 83 399 24 854 1260 1686
Cuts at: 5217 6606 7564 442
Size: 1389 958 1753
Fragments arranged by size:
1753 1686 1389 1260 958 854 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2912 3146 3450 7535 1239
Size: 1673 234 304 4085 2579
Fragments arranged by size:
4085 2579 1673 304 234
Bpu10I CC'TnA_GC
Cuts at: 3659 3659
Size: 8875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7000 7865 8163 7000
Size: 865 298 7712
Fragments arranged by size:
7712 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3342 5463 7538 882
Size: 617 1843 2121 2075 2219
Fragments arranged by size:
2219 2121 2075 1843 617
BsaAI yAC'GTr
Cuts at: 493 1204 6666 493
Size: 711 5462 2702
Fragments arranged by size:
5462 2702 711
BsaBI GATnn'nnATC
Cuts at: 3249 8869 3249
Size: 5620 3255
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6682 7064 7699 275
Size: 53 83 186 936 5149 382 635 1451
Fragments arranged by size:
5149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2574 7308 7706 7980 8127 8538 2574
Size: 4734 398 274 147 411 2911
Fragments arranged by size:
4734 2911 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2538 2568 1178
Size: 30 165 30 1135 30 7485
Fragments arranged by size:
7485 1135 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 4926 5810 5856 1186
Size: 894 2846 884 46 4205
Fragments arranged by size:
4205 2846 894 884 46
BseYI C'CCAG_C
Cuts at: 2510 2985 3759 4138 8078 8574 2510
Size: 475 774 379 3940 496 2811
Fragments arranged by size:
3940 2811 774 496 475 379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 3817 5096 5222 1163
Size: 573 345 1736 1279 126 4816
Fragments arranged by size:
4816 1736 1279 573 345 126
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3647 6325 7086 8114 1540
Size: 275 186 1646 2678 761 1028 2301
Fragments arranged by size:
2678 2301 1646 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 3192 3724 3857 4280 5176 5820 6048 6936
Size: 1146 532 133 423 896 644 228 888
Cuts at: 6936 7021 8092 2046
Size: 85 1071 2829
Fragments arranged by size:
2829 1146 1071 896 888 644 532 423
228 133 85
BsmI GAATG_Cn'
Cuts at: 2638 2869 3095 3659 6386 6479 2638
Size: 231 226 564 2727 93 5034
Fragments arranged by size:
5034 2727 564 231 226 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2511 2986 3192 3724 3857 4280
Size: 399 256 465 475 206 532 133 423
Cuts at: 4280 4963 5176 5223 5820 5848 6048 6194 6936
Size: 683 213 47 597 28 200 146 742
Cuts at: 6936 7021 8092 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 683 597 532 475 465
423 399 256 213 206 200 146 133
85 47 28
BspEI T'CCGG_A
Cuts at: 8538 8538
Size: 8875
BspHI T'CATG_A
Cuts at: 3184 5394 6764 8494 3184
Size: 2210 1370 1730 3565
Fragments arranged by size:
3565 2210 1730 1370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2432 3385 4710 6230 844
Size: 1588 953 1325 1520 3489
Fragments arranged by size:
3489 1588 1520 1325 953
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 6762 1394
Size: 429 1840 3099 3507
Fragments arranged by size:
3507 3099 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2077 2633 4570 7370 7544 65
Size: 1183 829 556 1937 2800 174 1396
Fragments arranged by size:
2800 1937 1396 1183 829 556 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2574 7519 7706 1700
Size: 110 764 4945 187 2869
Fragments arranged by size:
4945 2869 764 187 110
BsrGI T'GTAC_A
Cuts at: 96 4510 5713 96
Size: 4414 1203 3258
Fragments arranged by size:
4414 3258 1203
BssHII G'CGCG_C
Cuts at: 1925 3236 1925
Size: 1311 7564
BssSI C'ACGA_G
Cuts at: 2039 6935 7947 2039
Size: 4896 1012 2967
Fragments arranged by size:
4896 2967 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3588 794
Size: 2794 6081
BstBI TT'CG_AA
Cuts at: 5500 5512 7679 5500
Size: 12 2167 6696
Fragments arranged by size:
6696 2167 12
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8875
BstXI CCAn_nnnn'nTGG
Cuts at: 5748 5748
Size: 8875
BstYI r'GATC_y
Cuts at: 1972 2924 3540 3681 3970 4225 4479 4891 5494
Size: 952 616 141 289 255 254 412 603
Cuts at: 5494 5542 6205 6572 6957 6974 8426 8512 8524
Size: 48 663 367 385 17 1452 86 12
Cuts at: 8524 8870 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 952 663 616 603 412 385
367 346 289 255 254 141 86 48
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2145 3934 513
Size: 552 1080 1789 5454
Fragments arranged by size:
5454 1789 1080 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4364 487
Size: 683 56 3138 4998
Fragments arranged by size:
4998 3138 683 56
BtsI GCAGTG_nn'
Cuts at: 2479 3190 4906 5205 6391 7185 7205 2479
Size: 711 1716 299 1186 794 20 4149
Fragments arranged by size:
4149 1716 1186 794 711 299 20
ClaI AT'CG_AT
Cuts at: 1554 3079 1554
Size: 1525 7350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8840
DraI TTT'AAA
Cuts at: 2223 3798 4101 6179 2223
Size: 1575 303 2078 4919
Fragments arranged by size:
4919 2078 1575 303
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2698 5095 7882 809
Size: 507 1382 2397 2787 1802
Fragments arranged by size:
2787 2397 1802 1382 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3931 6322 7211
Size: 54 1425 50 275 18 2101 2391 889
Cuts at: 7211 8840 8
Size: 1629 43
Fragments arranged by size:
2391 2101 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6322 1537
Size: 275 4510 4090
Fragments arranged by size:
4510 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2499 5254 6805 1353
Size: 1146 2755 1551 3423
Fragments arranged by size:
3423 2755 1551 1146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7462 7836 7982 387
Size: 1064 138 5873 374 146 1280
Fragments arranged by size:
5873 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2912 2939 3146 3450 4195 4626
Size: 393 590 690 27 207 304 745 431
Cuts at: 4626 4692 6938 7535 8322 8590 1239
Size: 66 2246 597 787 268 1524
Fragments arranged by size:
2246 1524 787 745 690 597 590 431
393 304 268 207 66 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2240 2240
Size: 8875
EcoO109I rG'GnC_Cy
Cuts at: 2011 2421 2982 2011
Size: 410 561 7904
Fragments arranged by size:
7904 561 410
EcoRI G'AATT_C
Cuts at: 4060 4306 6184 6562 4060
Size: 246 1878 378 6373
Fragments arranged by size:
6373 1878 378 246
EcoRV GAT'ATC
Cuts at: 2646 6015 2646
Size: 3369 5506
FspI TGC'GCA
Cuts at: 7381 7381
Size: 8875
HaeII r_GCGC'y
Cuts at: 1678 2541 8022 1678
Size: 863 5481 2531
Fragments arranged by size:
5481 2531 863
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 2538 2570 3658
Size: 32 135 32 881 32 53 32 1088
Cuts at: 3658 3690 5512 5544 5789 5821 7516 7548 7590
Size: 32 1822 32 245 32 1695 32 42
Cuts at: 7590 7622 1373
Size: 32 2626
Fragments arranged by size:
2626 1822 1695 1088 881 245 135 53
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2371 2413 3115 3139 3286 4376
Size: 988 281 433 42 702 24 147 1090
Cuts at: 4376 6219 6465 669
Size: 1843 246 3079
Fragments arranged by size:
3079 1843 1090 988 702 433 281 246
147 42 24
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8875
HpaI GTT'AAC
Cuts at: 6465 6465
Size: 8875
KpnI G_GTAC'C
Cuts at: 6200 6200
Size: 8875
MfeI C'AATT_G
Cuts at: 4077 6474 4077
Size: 2397 6478
MluI A'CGCG_T
Cuts at: 8751 8751
Size: 8875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2451 4274 4615 5277 5555 7989 8173 1305
Size: 1146 1823 341 662 278 2434 184 2007
Fragments arranged by size:
2434 2007 1823 1146 662 341 278 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3933 10
Size: 54 1425 343 2101 4952
Fragments arranged by size:
4952 2101 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2354 2882 3871 4337 5244 5415
Size: 648 873 315 528 989 466 907 171
Cuts at: 5415 6833 7192 7351 518
Size: 1418 359 159 2042
Fragments arranged by size:
2042 1418 989 907 873 648 528 466
359 315 171 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2147 4429 5464 6291 6968 8116 8361
Size: 412 129 2282 1035 827 677 1148 245
Cuts at: 8361 1606
Size: 2120
Fragments arranged by size:
2282 2120 1148 1035 827 677 412 245
129
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8323
NdeI CA'TA_TG
Cuts at: 387 3752 387
Size: 3365 5510
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8875
NotI GC'GGCC_GC
Cuts at: 6322 6322
Size: 8875
NspI r_CATG'y
Cuts at: 1132 1229 2438 4095 4374 5098 5210 5692 6233
Size: 97 1209 1657 279 724 112 482 541
Cuts at: 6233 7778 1132
Size: 1545 2229
Fragments arranged by size:
2229 1657 1545 1209 724 541 482 279
112 97
PasI CC'CwG_GG
Cuts at: 1348 2542 1348
Size: 1194 7681
PciI A'CATG_T
Cuts at: 2434 4091 5094 5688 7774 2434
Size: 1657 1003 594 2086 3535
Fragments arranged by size:
3535 2086 1657 1003 594
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3551 1108
Size: 2443 6432
PfoI T'CCnGG_A
Cuts at: 3444 6008 3444
Size: 2564 6311
PmeI GTTT'AAAC
Cuts at: 6179 6179
Size: 8875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6944 6976 8457 8489 6944
Size: 32 1481 32 7330
Fragments arranged by size:
7330 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2421 2421
Size: 8875
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8875
PsiI TTA'TAA
Cuts at: 6445 6445
Size: 8875
PspOMI G'GGCC_C
Cuts at: 2982 5219 2982
Size: 2237 6638
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3247 3279 3247
Size: 32 8843
PstI C_TGCA'G
Cuts at: 830 2309 3566 3584 6227 830
Size: 1479 1257 18 2643 3478
Fragments arranged by size:
3478 2643 1479 1257 18
PvuI CG_AT'CG
Cuts at: 2001 3647 2001
Size: 1646 7229
PvuII CAG'CTG
Cuts at: 4429 4429
Size: 8875
SacII CC_GC'GG
Cuts at: 2148 2148
Size: 8875
SalI G'TCGA_C
Cuts at: 1936 2369 6217 1936
Size: 433 3848 4594
Fragments arranged by size:
4594 3848 433
SapI GCTCTTCn'nnn_
Cuts at: 2499 2499
Size: 8875
SbfI CC_TGCA'GG
Cuts at: 6227 6227
Size: 8875
ScaI AGT'ACT
Cuts at: 7123 8835 7123
Size: 1712 7163
SfcI C'TryA_G
Cuts at: 826 1045 2305 3562 3580 4980 5004 5707 6223
Size: 219 1260 1257 18 1400 24 703 516
Cuts at: 6223 7358 8039 8230 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1400 1260 1257 1135 703 681 516
219 191 24 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8875
SmaI CCC'GGG
Cuts at: 1872 6202 1872
Size: 4330 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6979 7880 8142 820
Size: 197 926 5036 901 262 1553
Fragments arranged by size:
5036 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6666 493
Size: 6173 2702
SpeI A'CTAG_T
Cuts at: 152 5111 152
Size: 4959 3916
SphI G_CATG'C
Cuts at: 1132 6233 1132
Size: 5101 3774
SspI AAT'ATT
Cuts at: 5 52 6799 5
Size: 47 6747 2081
Fragments arranged by size:
6747 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8875
StyI C'CwwG_G
Cuts at: 513 1065 2055 2194 3817 4762 6314 513
Size: 552 990 139 1623 945 1552 3074
Fragments arranged by size:
3074 1623 1552 990 945 552 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2170 2295 5396 6902 6919 7072 7257
Size: 1255 590 125 3101 1506 17 153 185
Cuts at: 7257 7702 325
Size: 445 1498
Fragments arranged by size:
3101 1506 1498 1255 590 445 185 153
125 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 3953 4094
Size: 274 80 33 51 1443 276 1700 141
Cuts at: 4094 4510 4573 4927 5713 7121 8833 96
Size: 416 63 354 786 1408 1712 138
Fragments arranged by size:
1712 1700 1443 1408 786 416 354 276
274 141 138 80 63 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2051 3868 3900 8835 8867 2019
Size: 32 1817 32 4935 32 2027
Fragments arranged by size:
4935 2027 1817 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8875
XbaI T'CTAG_A
Cuts at: 2809 4064 6211 2809
Size: 1255 2147 5473
Fragments arranged by size:
5473 2147 1255
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6159 6159
Size: 8875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8875
XmaI C'CCGG_G
Cuts at: 1870 6200 1870
Size: 4330 4545
XmnI GAAnn'nnTTC
Cuts at: 2235 4908 7004 2235
Size: 2673 2096 4106
Fragments arranged by size:
4106 2673 2096
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6683 276
Size: 53 83 186 936 5149 2468
Fragments arranged by size:
5149 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI RsrII SacI SanDI SexAI
SfiI SfoI SrfI SwaI