Restriction map for
pFN21AE3464
(Circular) (Six-base) MAPSORT of: pFN21AE3464.seq Check: 6950 from: 1 to: 8815
pFN21AE3464 8815 bp
With 182 enzymes: SgfI *
March 28, 2012 12:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2781 5222 2781
Size: 2441 6374
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3062 4818 6625 278
Size: 53 83 186 936 1526 1756 1807 2468
Fragments arranged by size:
2468 1807 1756 1526 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3681 6158 1937
Size: 1744 2477 4594
Fragments arranged by size:
4594 2477 1744
Acc65I G'GTAC_C
Cuts at: 2567 6136 2567
Size: 3569 5246
AclI AA'CG_TT
Cuts at: 1563 6942 7315 1563
Size: 5379 373 3063
Fragments arranged by size:
5379 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2321 2836 3482 3944 4058 4098 4184 4527
Size: 689 515 646 462 114 40 86 343
Cuts at: 4527 4855 5321 5612 6878 8262 8530 1632
Size: 328 466 291 1266 1384 268 1917
Fragments arranged by size:
1917 1384 1266 689 646 515 466 462
343 328 291 268 114 86 40
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8618
AflIII A'CryG_T
Cuts at: 1203 5622 7714 8691 1203
Size: 4419 2092 977 1327
Fragments arranged by size:
4419 2092 1327 977
AgeI A'CCGG_T
Cuts at: 7646 7646
Size: 8815
AhdI GACnn_n'nnGTC
Cuts at: 2640 4819 5626 7544 2640
Size: 2179 807 1918 3911
Fragments arranged by size:
3911 2179 1918 807
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4448 5573 1184
Size: 3264 1125 4426
Fragments arranged by size:
4426 3264 1125
AlwNI CAG_nnn'CTG
Cuts at: 2859 2869 4162 5333 6558 8130 2859
Size: 10 1293 1171 1225 1572 3544
Fragments arranged by size:
3544 1572 1293 1225 1171 10
ApaLI G'TGCA_C
Cuts at: 2986 6872 8028 2986
Size: 3886 1156 3773
Fragments arranged by size:
3886 3773 1156
ApoI r'AATT_y
Cuts at: 1493 2968 3174 3506 5708 6124 6350 6502 8611
Size: 1475 206 332 2202 416 226 152 2109
Cuts at: 8611 1493
Size: 1697
Fragments arranged by size:
2202 2109 1697 1475 416 332 226 206
152
AseI AT'TA_AT
Cuts at: 160 1032 7369 160
Size: 872 6337 1606
Fragments arranged by size:
6337 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8815
AvaI C'yCGr_G
Cuts at: 1870 1943 2291 2424 4048 5025 5903 6140 1870
Size: 73 348 133 1624 977 878 237 4545
Fragments arranged by size:
4545 1624 977 878 348 237 133 73
BamHI G'GATC_C
Cuts at: 3883 6145 6512 3883
Size: 2262 367 6186
Fragments arranged by size:
6186 2262 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2567 3435 5578 6136
Size: 325 217 627 288 492 868 2143 558
Cuts at: 6136 7591 618
Size: 1455 1842
Fragments arranged by size:
2143 1842 1455 868 627 558 492 325
288 217
BanII G_rGCy'C
Cuts at: 1391 2292 2460 3075 4599 5066 6134 1391
Size: 901 168 615 1524 467 1068 4072
Fragments arranged by size:
4072 1524 1068 901 615 467 168
BbeI G_GCGC'C
Cuts at: 2079 3439 2079
Size: 1360 7455
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3047 3470 3791 4002 4199 5144 5596
Size: 971 1148 423 321 211 197 945 452
Cuts at: 5596 928
Size: 4147
Fragments arranged by size:
4147 1148 971 945 452 423 321 211
197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4312 4346 4483 4517 4663 4697 7006
Size: 34 2375 34 137 34 146 34 2309
Cuts at: 7006 7040 1903
Size: 34 3678
Fragments arranged by size:
3678 2375 2309 146 137 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2501 6707 7917 2501
Size: 4206 1210 3399
Fragments arranged by size:
4206 3399 1210
BclI T'GATC_A
Cuts at: 1548 2253 3105 1548
Size: 705 852 7258
Fragments arranged by size:
7258 852 705
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3562 4924 7426
Size: 107 122 71 631 605 1890 1362 2502
Cuts at: 7426 136
Size: 1525
Fragments arranged by size:
2502 1890 1525 1362 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3537 8810 3537
Size: 5273 3542
BlpI GC'TnA_GC
Cuts at: 3141 6232 3141
Size: 3091 5724
Bme1580I G_kGCm'C
Cuts at: 1790 2990 3879 4293 4335 5952 6876 8032 1790
Size: 1200 889 414 42 1617 924 1156 2573
Fragments arranged by size:
2573 1617 1200 1156 924 889 414 42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8815
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2081 3760 6546
Size: 416 53 83 399 24 664 1679 2786
Cuts at: 6546 7504 442
Size: 958 1753
Fragments arranged by size:
2786 1753 1679 958 664 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8815
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 2968 4198 2179
Size: 789 1230 6796
Fragments arranged by size:
6796 1230 789
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 3346 4193 5840 5939 7475 1239
Size: 935 1172 847 1647 99 1536 2579
Fragments arranged by size:
2579 1647 1536 1172 935 847 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2372 3737 5822 6940 7805 8103 2372
Size: 1365 2085 1118 865 298 3084
Fragments arranged by size:
3084 2085 1365 1118 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2220 2795 3251 3573 3755 4065 5114
Size: 617 721 575 456 322 182 310 1049
Cuts at: 5114 5289 7478 882
Size: 175 2189 2219
Fragments arranged by size:
2219 2189 1049 721 617 575 456 322
310 182 175
BsaAI yAC'GTr
Cuts at: 493 1204 4798 5002 5224 6606 493
Size: 711 3594 204 222 1382 2702
Fragments arranged by size:
3594 2702 1382 711 222 204
BsaBI GATnn'nnATC
Cuts at: 2417 8809 2417
Size: 6392 2423
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 3059 3436 4815
Size: 53 83 186 936 543 983 377 1379
Cuts at: 4815 6622 7004 7639 275
Size: 1807 382 635 1451
Fragments arranged by size:
1807 1451 1379 983 936 635 543 382
377 186 83 53
BsaWI w'CCGG_w
Cuts at: 2124 3231 4342 5538 5581 7248 7646 7920 8067
Size: 1107 1111 1196 43 1667 398 274 147
Cuts at: 8067 8478 2124
Size: 411 2461
Fragments arranged by size:
2461 1667 1196 1111 1107 411 398 274
147 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4045 4075 4169 4199 5912
Size: 30 165 30 2642 30 94 30 1713
Cuts at: 5912 5942 1178
Size: 30 4051
Fragments arranged by size:
4051 2642 1713 165 94 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2558 3135 3267 4190 4361 4664 5903 5927
Size: 1372 577 132 923 171 303 1239 24
Cuts at: 5927 5996 1186
Size: 69 4005
Fragments arranged by size:
4005 1372 1239 923 577 303 171 132
69 24
BseYI C'CCAG_C
Cuts at: 4930 5053 5855 6013 8018 8514 4930
Size: 123 802 158 2005 496 5231
Fragments arranged by size:
5231 2005 802 496 158 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2671 2855 4196 4489 5834 1163
Size: 573 935 184 1341 293 1345 4144
Fragments arranged by size:
4144 1345 1341 935 573 293 184
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 4122 6265 7026 8054 1540
Size: 275 186 36 2085 2143 761 1028 2301
Fragments arranged by size:
2301 2143 2085 1028 761 275 186 36
BsiHKAI G_wGCw'C
Cuts at: 2460 2990 3075 4353 6876 6961 8032 2460
Size: 530 85 1278 2523 85 1071 3243
Fragments arranged by size:
3243 2523 1278 1071 530 85 85
BsmI GAATG_Cn'
Cuts at: 4133 6326 6419 4133
Size: 2193 93 6529
Fragments arranged by size:
6529 2193 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4672 4672
Size: 8815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2292 2460 2990 3075 3879 4293 4335
Size: 399 502 168 530 85 804 414 42
Cuts at: 4335 4353 4599 5066 5952 6134 6876 6961 8032
Size: 18 246 467 886 182 742 85 1071
Cuts at: 8032 1391
Size: 2174
Fragments arranged by size:
2174 1071 886 804 742 530 502 467
414 399 246 182 168 85 85 42
18
BspEI T'CCGG_A
Cuts at: 2124 3231 4342 8478 2124
Size: 1107 1111 4136 2461
Fragments arranged by size:
4136 2461 1111 1107
BspHI T'CATG_A
Cuts at: 2250 5199 6704 8434 2250
Size: 2949 1505 1730 2631
Fragments arranged by size:
2949 2631 1730 1505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2781 2813 2877 4218 4242 5222 5841 5856
Size: 1937 32 64 1341 24 980 619 15
Cuts at: 5856 6170 844
Size: 314 3489
Fragments arranged by size:
3489 1937 1341 980 619 314 64 32
24 15
BsrBI CCG'CTC
Cuts at: 1394 1823 5170 6702 1394
Size: 429 3347 1532 3507
Fragments arranged by size:
3507 3347 1532 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3453 5095 7310 7484 65
Size: 1183 2205 1642 2215 174 1396
Fragments arranged by size:
2215 2205 1642 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2019 4121 7459 7646 1700
Size: 110 209 2102 3338 187 2869
Fragments arranged by size:
3338 2869 2102 209 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8815
BssSI C'ACGA_G
Cuts at: 4336 6875 7887 4336
Size: 2539 1012 5264
Fragments arranged by size:
5264 2539 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2994 5396 794
Size: 2200 2402 4213
Fragments arranged by size:
4213 2402 2200
BstBI TT'CG_AA
Cuts at: 7619 7619
Size: 8815
BstEII G'GTnAC_C
Cuts at: 1184 3793 1184
Size: 2609 6206
BstXI CCAn_nnnn'nTGG
Cuts at: 2761 2761
Size: 8815
BstYI r'GATC_y
Cuts at: 1972 3537 3883 4158 6145 6512 6897 6914 8366
Size: 1565 346 275 1987 367 385 17 1452
Cuts at: 8366 8452 8464 8810 1972
Size: 86 12 346 1977
Fragments arranged by size:
1987 1977 1565 1452 385 367 346 346
275 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3308 3698 4458 3308
Size: 390 760 7665
Fragments arranged by size:
7665 760 390
BtgI C'CryG_G
Cuts at: 513 1065 2004 2079 2502 4293 513
Size: 552 939 75 423 1791 5035
Fragments arranged by size:
5035 1791 939 552 423 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4278 487
Size: 683 56 3052 5024
Fragments arranged by size:
5024 3052 683 56
BtsI GCAGTG_nn'
Cuts at: 2644 4477 6331 7125 7145 2644
Size: 1833 1854 794 20 4314
Fragments arranged by size:
4314 1854 1833 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4738 4773 5250 5285 529
Size: 35 4174 35 477 35 4059
Fragments arranged by size:
4174 4059 477 35 35 35
DraI TTT'AAA
Cuts at: 6119 6119
Size: 8815
DraIII CAC_nnn'GTG
Cuts at: 2757 4219 5227 2757
Size: 1462 1008 6345
Fragments arranged by size:
6345 1462 1008
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7822 809
Size: 507 6506 1802
Fragments arranged by size:
6506 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2034 2058
Size: 54 1425 50 275 18 177 27 24
Cuts at: 2058 2469 4119 4284 5324 5347 6262 7151 8780
Size: 411 1650 165 1040 23 915 889 1629
Cuts at: 8780 8
Size: 43
Fragments arranged by size:
1650 1629 1425 1040 915 889 411 275
177 165 54 50 43 27 24 23
18
EagI C'GGCC_G
Cuts at: 1537 1812 2034 4119 6262 1537
Size: 275 222 2085 2143 4090
Fragments arranged by size:
4090 2143 2085 275 222
EarI CTCTTCn'nnn_
Cuts at: 1353 2279 3368 4874 5297 6745 1353
Size: 926 1089 1506 423 1448 3423
Fragments arranged by size:
3423 1506 1448 1089 926 423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3185 4007 6097 7402 7776 7922
Size: 1064 138 1596 822 2090 1305 374 146
Cuts at: 7922 387
Size: 1280
Fragments arranged by size:
2090 1596 1305 1280 1064 822 374 146
138
EcoICRI GAG'CTC
Cuts at: 2458 3073 2458
Size: 615 8200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2321 2836 3346 3482 3944 4058
Size: 393 542 147 515 510 136 462 114
Cuts at: 4058 4098 4184 4193 4527 4855 5321 5612 5840
Size: 40 86 9 334 328 466 291 228
Cuts at: 5840 5939 6878 7475 8262 8530 1239
Size: 99 939 597 787 268 1524
Fragments arranged by size:
1524 939 787 597 542 515 510 466
462 393 334 328 291 268 228 147
136 114 99 86 40 9
EcoNI CCTnn'n_nnAGG
Cuts at: 4186 5186 5679 5837 4186
Size: 1000 493 158 7164
Fragments arranged by size:
7164 1000 493 158
EcoO109I rG'GnC_Cy
Cuts at: 2305 2866 3002 3613 4053 4254 5443 6006 2305
Size: 561 136 611 440 201 1189 563 5114
Fragments arranged by size:
5114 1189 611 563 561 440 201 136
EcoRI G'AATT_C
Cuts at: 2968 6124 6502 2968
Size: 3156 378 5281
Fragments arranged by size:
5281 3156 378
EcoRV GAT'ATC
Cuts at: 2122 2122
Size: 8815
FspI TGC'GCA
Cuts at: 5777 7321 5777
Size: 1544 7271
FspAI rTGC'GCAy
Cuts at: 5777 5777
Size: 8815
HaeII r_GCGC'y
Cuts at: 1678 2079 2842 3439 7962 1678
Size: 401 763 597 4523 2531
Fragments arranged by size:
4523 2531 763 597 401
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2626 2658 3072 3104 3467
Size: 32 135 32 1054 32 414 32 363
Cuts at: 3467 3499 3701 3733 3765 3875 3907 4045 4077
Size: 32 202 32 32 110 32 138 32
Cuts at: 4077 4138 4170 4805 4837 5019 5051 5612 5644
Size: 61 32 635 32 182 32 561 32
Cuts at: 5644 5948 5980 5990 6022 6082 6114 7456 7488
Size: 304 32 10 32 60 32 1342 32
Cuts at: 7488 7530 7562 1373
Size: 42 32 2626
Fragments arranged by size:
2626 1342 1054 635 561 414 363 304
202 182 138 135 110 61 60 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 5146 5830 6159 6405 669
Size: 988 281 3208 684 329 246 3079
Fragments arranged by size:
3208 3079 988 684 329 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8815
HpaI GTT'AAC
Cuts at: 6405 6405
Size: 8815
KasI G'GCGC_C
Cuts at: 2075 3435 2075
Size: 1360 7455
KpnI G_GTAC'C
Cuts at: 2571 6140 2571
Size: 3569 5246
MfeI C'AATT_G
Cuts at: 6414 6414
Size: 8815
MluI A'CGCG_T
Cuts at: 8691 8691
Size: 8815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2612 3650 3687 3729 3966 7929 8113 1305
Size: 1307 1038 37 42 237 3963 184 2007
Fragments arranged by size:
3963 2007 1307 1038 237 184 42 37
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2471 10
Size: 54 1425 343 177 462 6354
Fragments arranged by size:
6354 1425 462 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3779 5777 6056 6773 7132 7291 518
Size: 648 2613 1998 279 717 359 159 2042
Fragments arranged by size:
2613 2042 1998 717 648 359 279 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2081 3140 4930 6231 6908 8056 8301
Size: 438 37 1059 1790 1301 677 1148 245
Cuts at: 8301 1606
Size: 2120
Fragments arranged by size:
2120 1790 1301 1148 1059 677 438 245
37
NaeI GCC'GGC
Cuts at: 2021 4123 2021
Size: 2102 6713
NarI GG'CG_CC
Cuts at: 2076 3436 2076
Size: 1360 7455
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7324
Fragments arranged by size:
7324 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8815
NgoMIV G'CCGG_C
Cuts at: 2019 4121 2019
Size: 2102 6713
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8815
NotI GC'GGCC_GC
Cuts at: 2034 6262 2034
Size: 4228 4587
NspI r_CATG'y
Cuts at: 1132 1229 5626 5782 6173 7718 1132
Size: 97 4397 156 391 1545 2229
Fragments arranged by size:
4397 2229 1545 391 156 97
PasI CC'CwG_GG
Cuts at: 1348 3731 1348
Size: 2383 6432
PciI A'CATG_T
Cuts at: 5622 7714 5622
Size: 2092 6723
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3409 3669 3970 5224 1108
Size: 2301 260 301 1254 4699
Fragments arranged by size:
4699 2301 1254 301 260
PfoI T'CCnGG_A
Cuts at: 2493 2829 5817 2493
Size: 336 2988 5491
Fragments arranged by size:
5491 2988 336
PmeI GTTT'AAAC
Cuts at: 6119 6119
Size: 8815
PmlI CAC'GTG
Cuts at: 5224 5224
Size: 8815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2385 2417 5858 5890 6884 6916 8397 8429 2385
Size: 32 3441 32 994 32 1481 32 2771
Fragments arranged by size:
3441 2771 1481 994 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 2866 3002 4053 4254 5443 6006 2305
Size: 561 136 1051 201 1189 563 5114
Fragments arranged by size:
5114 1189 1051 563 561 201 136
PshAI GACnn'nnGTC
Cuts at: 1613 2559 3791 5748 1613
Size: 946 1232 1957 4680
Fragments arranged by size:
4680 1957 1232 946
PsiI TTA'TAA
Cuts at: 6385 6385
Size: 8815
PspXI vC'TCGA_Gb
Cuts at: 4048 4048
Size: 8815
PstI C_TGCA'G
Cuts at: 830 4437 4703 6167 830
Size: 3607 266 1464 3478
Fragments arranged by size:
3607 3478 1464 266
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8815
PvuII CAG'CTG
Cuts at: 3140 3140
Size: 8815
SacI G_AGCT'C
Cuts at: 2460 3075 2460
Size: 615 8200
SacII CC_GC'GG
Cuts at: 2082 2082
Size: 8815
SalI G'TCGA_C
Cuts at: 1936 6157 1936
Size: 4221 4594
SanDI GG'GwC_CC
Cuts at: 3002 3002
Size: 8815
SapI GCTCTTCn'nnn_
Cuts at: 2279 5297 2279
Size: 3018 5797
SbfI CC_TGCA'GG
Cuts at: 6167 6167
Size: 8815
ScaI AGT'ACT
Cuts at: 7063 8775 7063
Size: 1712 7103
SexAI A'CCwGG_T
Cuts at: 3969 4055 3969
Size: 86 8729
SfcI C'TryA_G
Cuts at: 826 1045 4433 4699 4729 6163 7298 7979 8170
Size: 219 3388 266 30 1434 1135 681 191
Cuts at: 8170 826
Size: 1471
Fragments arranged by size:
3388 1471 1434 1135 681 266 219 191
30
SfoI GGC'GCC
Cuts at: 2077 3437 2077
Size: 1360 7455
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8815
SmaI CCC'GGG
Cuts at: 1872 2426 6142 1872
Size: 554 3716 4545
Fragments arranged by size:
4545 3716 554
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2291 2387 3752 4048 5025 5837
Size: 197 926 348 96 1365 296 977 812
Cuts at: 5837 6919 7820 8082 820
Size: 1082 901 262 1553
Fragments arranged by size:
1553 1365 1082 977 926 901 812 348
296 262 197 96
SnaBI TAC'GTA
Cuts at: 493 6606 493
Size: 6113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8815
SphI G_CATG'C
Cuts at: 1132 6173 1132
Size: 5041 3774
SspI AAT'ATT
Cuts at: 5 52 4828 6739 5
Size: 47 4776 1911 2081
Fragments arranged by size:
4776 2081 1911 47
StuI AGG'CCT
Cuts at: 1517 2700 4357 5192 5685 1517
Size: 1183 1657 835 493 4647
Fragments arranged by size:
4647 1657 1183 835 493
StyI C'CwwG_G
Cuts at: 513 1065 2004 3012 3261 3291 3429 4258 4512
Size: 552 939 1008 249 30 138 829 254
Cuts at: 4512 5250 5680 6254 513
Size: 738 430 574 3074
Fragments arranged by size:
3074 1008 939 829 738 574 552 430
254 249 138 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3744 3977 5185 6090 6842 6859 7012
Size: 1255 2164 233 1208 905 752 17 153
Cuts at: 7012 7197 7642 325
Size: 185 445 1498
Fragments arranged by size:
2164 1498 1255 1208 905 752 445 233
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7061 8773 96
Size: 274 80 33 51 1443 5084 1712 138
Fragments arranged by size:
5084 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2777 2809 8775 8807 2777
Size: 32 5966 32 2785
Fragments arranged by size:
5966 2785 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4057 1316
Size: 2741 6074
XbaI T'CTAG_A
Cuts at: 6151 6151
Size: 8815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2758 2758
Size: 8815
XhoI C'TCGA_G
Cuts at: 1943 2291 4048 5025 1943
Size: 348 1757 977 5733
Fragments arranged by size:
5733 1757 977 348
XmaI C'CCGG_G
Cuts at: 1870 2424 6140 1870
Size: 554 3716 4545
Fragments arranged by size:
4545 3716 554
XmnI GAAnn'nnTTC
Cuts at: 3362 5207 6944 3362
Size: 1845 1737 5233
Fragments arranged by size:
5233 1845 1737
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3060 4816 6623 276
Size: 53 83 186 936 1526 1756 1807 2468
Fragments arranged by size:
2468 1807 1756 1526 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I FalI FseI NruI NsiI PacI
PspOMI PsrI RsrII SfiI SrfI SwaI