Restriction map for
pFN21AE3461
(Circular) (Six-base) MAPSORT of: pFN21AE3461.seq Check: 3208 from: 1 to: 8764
pFN21AE3461 8764 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4471 6574 278
Size: 53 83 186 936 2935 2103 2468
Fragments arranged by size:
2935 2468 2103 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4004 5418 6107 1937
Size: 2067 1414 689 4594
Fragments arranged by size:
4594 2067 1414 689
Acc65I G'GTAC_C
Cuts at: 2294 6085 2294
Size: 3791 4973
AclI AA'CG_TT
Cuts at: 1563 6891 7264 1563
Size: 5328 373 3063
Fragments arranged by size:
5328 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2376 2511 4029 4094 4667 4753 5066 6014
Size: 744 135 1518 65 573 86 313 948
Cuts at: 6014 6827 8211 8479 1632
Size: 813 1384 268 1917
Fragments arranged by size:
1917 1518 1384 948 813 744 573 313
268 135 86 65
AflII C'TTAA_G
Cuts at: 820 1017 3395 3518 820
Size: 197 2378 123 6066
Fragments arranged by size:
6066 2378 197 123
AflIII A'CryG_T
Cuts at: 1203 5061 7663 8640 1203
Size: 3858 2602 977 1327
Fragments arranged by size:
3858 2602 1327 977
AgeI A'CCGG_T
Cuts at: 3828 7595 3828
Size: 3767 4997
AhdI GACnn_n'nnGTC
Cuts at: 7493 7493
Size: 8764
AleI CACnn'nnGTG
Cuts at: 1166 2265 1166
Size: 1099 7665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2571 1184
Size: 1387 7377
AlwNI CAG_nnn'CTG
Cuts at: 3797 4370 5464 6507 8079 3797
Size: 573 1094 1043 1572 4482
Fragments arranged by size:
4482 1572 1094 1043 573
ApaI G_GGCC'C
Cuts at: 5916 5976 5916
Size: 60 8704
ApaLI G'TGCA_C
Cuts at: 6821 7977 6821
Size: 1156 7608
ApoI r'AATT_y
Cuts at: 1493 3758 4143 5201 5241 5398 5474 6073 6299
Size: 2265 385 1058 40 157 76 599 226
Cuts at: 6299 6451 8560 1493
Size: 152 2109 1697
Fragments arranged by size:
2265 2109 1697 1058 599 385 226 157
152 76 40
AseI AT'TA_AT
Cuts at: 160 1032 2985 3879 7318 160
Size: 872 1953 894 3439 1606
Fragments arranged by size:
3439 1953 1606 894 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8764
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2883 3286 3489 5196 5220 6089
Size: 73 91 849 403 203 1707 24 869
Cuts at: 6089 1870
Size: 4545
Fragments arranged by size:
4545 1707 869 849 403 203 91 73
24
BamHI G'GATC_C
Cuts at: 2125 2251 6094 6461 2125
Size: 126 3843 367 4428
Fragments arranged by size:
4428 3843 367 126
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2273 2294 2330 3316 3749
Size: 325 217 627 486 21 36 986 433
Cuts at: 3749 6085 7540 618
Size: 2336 1455 1842
Fragments arranged by size:
2336 1842 1455 986 627 486 433 325
217 36 21
BanII G_rGCy'C
Cuts at: 1391 2306 4091 5345 5636 5853 5916 5976 6083
Size: 915 1785 1254 291 217 63 60 107
Cuts at: 6083 1391
Size: 4072
Fragments arranged by size:
4072 1785 1254 915 291 217 107 63
60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3654 4851 928
Size: 971 1755 1197 4841
Fragments arranged by size:
4841 1755 1197 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3267 3301 6955 6989 1903
Size: 34 1330 34 3654 34 3678
Fragments arranged by size:
3678 3654 1330 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2133 2611 4894 5148 5448 6656 7866 2133
Size: 478 2283 254 300 1208 1210 3031
Fragments arranged by size:
3031 2283 1210 1208 478 300 254
BclI T'GATC_A
Cuts at: 1548 3922 4897 1548
Size: 2374 975 5415
Fragments arranged by size:
5415 2374 975
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5980 7375 136
Size: 107 122 71 631 605 4308 1395 1525
Fragments arranged by size:
4308 1525 1395 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8759 8759
Size: 8764
BlpI GC'TnA_GC
Cuts at: 3457 6181 3457
Size: 2724 6040
Bme1580I G_kGCm'C
Cuts at: 1790 5916 5976 5994 6825 7981 1790
Size: 4126 60 18 831 1156 2573
Fragments arranged by size:
4126 2573 1156 831 60 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8764
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5946 6495 7453
Size: 416 53 83 399 24 4529 549 958
Cuts at: 7453 442
Size: 1753
Fragments arranged by size:
4529 1753 958 549 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2723 3291 4937 5758 5773 7424 1239
Size: 1484 568 1646 821 15 1651 2579
Fragments arranged by size:
2579 1651 1646 1484 821 568 15
Bpu10I CC'TnA_GC
Cuts at: 3240 4101 3240
Size: 861 7903
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2528 3244 6889 7754 8052 2528
Size: 716 3645 865 298 3240
Fragments arranged by size:
3645 3240 865 716 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4826 5704 7427 882
Size: 617 3327 878 1723 2219
Fragments arranged by size:
3327 2219 1723 878 617
BsaAI yAC'GTr
Cuts at: 493 1204 6555 493
Size: 711 5351 2702
Fragments arranged by size:
5351 2702 711
BsaBI GATnn'nnATC
Cuts at: 2256 4431 5253 8758 2256
Size: 2175 822 3505 2262
Fragments arranged by size:
3505 2262 2175 822
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3651 4468 6571 6953
Size: 53 83 186 936 2118 817 2103 382
Cuts at: 6953 7588 275
Size: 635 1451
Fragments arranged by size:
2118 2103 1451 936 817 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2106 3828 7197 7595 7869 8016 8427 2106
Size: 1722 3369 398 274 147 411 2443
Fragments arranged by size:
3369 2443 1722 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5771 5801 1178
Size: 30 165 30 4368 30 4141
Fragments arranged by size:
4368 4141 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3900 5162 5636 1186
Size: 2714 1262 474 4314
Fragments arranged by size:
4314 2714 1262 474
BseYI C'CCAG_C
Cuts at: 2440 2558 4190 4616 5637 5646 5915 7967 8463
Size: 118 1632 426 1021 9 269 2052 496
Cuts at: 8463 2440
Size: 2741
Fragments arranged by size:
2741 2052 1632 1021 496 426 269 118
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3213 3230 5618 1163
Size: 573 1477 17 2388 4309
Fragments arranged by size:
4309 2388 1477 573 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4733 5558 6214 6975 8003 1540
Size: 275 186 2732 825 656 761 1028 2301
Fragments arranged by size:
2732 2301 1028 825 761 656 275 186
BsiHKAI G_wGCw'C
Cuts at: 2884 3220 5636 5853 6825 6910 7981 2884
Size: 336 2416 217 972 85 1071 3667
Fragments arranged by size:
3667 2416 1071 972 336 217 85
BsmI GAATG_Cn'
Cuts at: 3679 5046 6275 6368 3679
Size: 1367 1229 93 6075
Fragments arranged by size:
6075 1367 1229 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2670 2670
Size: 8764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2306 2884 3220 4091 5345 5636 5853
Size: 399 516 578 336 871 1254 291 217
Cuts at: 5853 5916 5976 5994 6083 6825 6910 7981 1391
Size: 63 60 18 89 742 85 1071 2174
Fragments arranged by size:
2174 1254 1071 871 742 578 516 399
336 291 217 89 85 63 60 18
BspEI T'CCGG_A
Cuts at: 8427 8427
Size: 8764
BspHI T'CATG_A
Cuts at: 5446 6653 8383 5446
Size: 1207 1730 5827
Fragments arranged by size:
5827 1730 1207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2582 4598 6119 844
Size: 1738 2016 1521 3489
Fragments arranged by size:
3489 2016 1738 1521
BsrBI CCG'CTC
Cuts at: 1394 1823 3305 5571 6651 1394
Size: 429 1482 2266 1080 3507
Fragments arranged by size:
3507 2266 1482 1080 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4072 4765 7259 7433 65
Size: 1183 2824 693 2494 174 1396
Fragments arranged by size:
2824 2494 1396 1183 693 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3828 5557 7408 7595 1700
Size: 110 2018 1729 1851 187 2869
Fragments arranged by size:
2869 2018 1851 1729 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8764
BssSI C'ACGA_G
Cuts at: 5346 6824 7836 5346
Size: 1478 1012 6274
Fragments arranged by size:
6274 1478 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4755 5989 794
Size: 3961 1234 3569
Fragments arranged by size:
3961 3569 1234
BstBI TT'CG_AA
Cuts at: 3001 7568 3001
Size: 4567 4197
BstEII G'GTnAC_C
Cuts at: 1184 2095 3824 1184
Size: 911 1729 6124
Fragments arranged by size:
6124 1729 911
BstXI CCAn_nnnn'nTGG
Cuts at: 2136 4519 2136
Size: 2383 6381
BstYI r'GATC_y
Cuts at: 1972 2125 2251 3794 6094 6461 6846 6863 8315
Size: 153 126 1543 2300 367 385 17 1452
Cuts at: 8315 8401 8413 8759 1972
Size: 86 12 346 1977
Fragments arranged by size:
2300 1977 1543 1452 385 367 346 153
126 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2289 2612 3978 4727 4733 4803 5316
Size: 552 1224 323 1366 749 6 70 513
Cuts at: 5316 5393 5589 513
Size: 77 196 3688
Fragments arranged by size:
3688 1366 1224 749 552 513 323 196
77 70 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2101 2135 487
Size: 683 56 875 34 7116
Fragments arranged by size:
7116 875 683 56 34
BtsI GCAGTG_nn'
Cuts at: 2241 2553 3355 6280 7074 7094 2241
Size: 312 802 2925 794 20 3911
Fragments arranged by size:
3911 2925 802 794 312 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8729
DraI TTT'AAA
Cuts at: 6068 6068
Size: 8764
DraIII CAC_nnn'GTG
Cuts at: 3208 5584 3208
Size: 2376 6388
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7771 809
Size: 507 6455 1802
Fragments arranged by size:
6455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2059 3498 3511
Size: 54 1425 50 275 18 229 1439 13
Cuts at: 3511 4730 5543 5555 6211 7100 8729 8
Size: 1219 813 12 656 889 1629 43
Fragments arranged by size:
1629 1439 1425 1219 889 813 656 275
229 54 50 43 18 13 12
EagI C'GGCC_G
Cuts at: 1537 1812 4730 5555 6211 1537
Size: 275 2918 825 656 4090
Fragments arranged by size:
4090 2918 825 656 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2421 2487 2730 4333 5139 6694 1353
Size: 1068 66 243 1603 806 1555 3423
Fragments arranged by size:
3423 1603 1555 1068 806 243 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5533 5887 7351 7725 7871 387
Size: 1064 138 3944 354 1464 374 146 1280
Fragments arranged by size:
3944 1464 1280 1064 374 354 146 138
EcoICRI GAG'CTC
Cuts at: 5634 5851 5634
Size: 217 8547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2376 2511 2723 3291 4029 4094 4667
Size: 393 744 135 212 568 738 65 573
Cuts at: 4667 4753 4937 5066 5758 5773 6014 6827 7424
Size: 86 184 129 692 15 241 813 597
Cuts at: 7424 8211 8479 1239
Size: 787 268 1524
Fragments arranged by size:
1524 813 787 744 738 692 597 573
568 393 268 241 212 184 135 129
86 65 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2578 5736 5858 6042 2578
Size: 3158 122 184 5300
Fragments arranged by size:
5300 3158 184 122
EcoO109I rG'GnC_Cy
Cuts at: 2849 4631 5912 5947 5972 5973 6051 2849
Size: 1782 1281 35 25 1 78 5562
Fragments arranged by size:
5562 1782 1281 78 35 25 1
EcoRI G'AATT_C
Cuts at: 5398 6073 6451 5398
Size: 675 378 7711
Fragments arranged by size:
7711 675 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3797 5992 3797
Size: 2195 6569
FspI TGC'GCA
Cuts at: 7270 7270
Size: 8764
HaeII r_GCGC'y
Cuts at: 1678 5653 7911 1678
Size: 3975 2258 2531
Fragments arranged by size:
3975 2531 2258
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2662 2694 3238 3242 3270
Size: 32 135 32 1090 32 544 4 28
Cuts at: 3270 3274 3411 3443 3779 3811 4014 4046 4061
Size: 4 137 32 336 32 203 32 15
Cuts at: 4061 4093 4310 4342 4764 4796 4868 4900 5446
Size: 32 217 32 422 32 72 32 546
Cuts at: 5446 5478 7405 7437 7479 7511 1373
Size: 32 1927 32 42 32 2626
Fragments arranged by size:
2626 1927 1090 546 544 422 336 217
203 137 135 72 42 32 32 32
32 32 32 32 32 32 32 32
32 32 28 15 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2526 6108 6354 669
Size: 988 281 588 3582 246 3079
Fragments arranged by size:
3582 3079 988 588 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8764
HpaI GTT'AAC
Cuts at: 6354 6354
Size: 8764
KpnI G_GTAC'C
Cuts at: 2298 6089 2298
Size: 3791 4973
MfeI C'AATT_G
Cuts at: 4517 6363 4517
Size: 1846 6918
MluI A'CGCG_T
Cuts at: 8640 8640
Size: 8764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2743 4104 4644 7878 8062 1305
Size: 1438 1361 540 3234 184 2007
Fragments arranged by size:
3234 2007 1438 1361 540 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6942
Fragments arranged by size:
6942 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2166 2265 3608 4344 6722 7081
Size: 648 843 157 99 1343 736 2378 359
Cuts at: 7081 7240 518
Size: 159 2042
Fragments arranged by size:
2378 2042 1343 843 736 648 359 159
157 99
MspA1I CmG'CkG
Cuts at: 1606 2534 2614 4234 4735 4805 5395 5608 5614
Size: 928 80 1620 501 70 590 213 6
Cuts at: 5614 6180 6857 8005 8250 1606
Size: 566 677 1148 245 2120
Fragments arranged by size:
2120 1620 1148 928 677 590 566 501
245 213 80 70 6
NaeI GCC'GGC
Cuts at: 5559 5559
Size: 8764
NcoI C'CATG_G
Cuts at: 513 1065 3978 5316 513
Size: 552 2913 1338 3961
Fragments arranged by size:
3961 2913 1338 552
NdeI CA'TA_TG
Cuts at: 387 3030 5358 387
Size: 2643 2328 3793
Fragments arranged by size:
3793 2643 2328
NgoMIV G'CCGG_C
Cuts at: 5557 5557
Size: 8764
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8764
NotI GC'GGCC_GC
Cuts at: 6211 6211
Size: 8764
NspI r_CATG'y
Cuts at: 1132 1229 2771 5065 6122 7667 1132
Size: 97 1542 2294 1057 1545 2229
Fragments arranged by size:
2294 2229 1545 1542 1057 97
PasI CC'CwG_GG
Cuts at: 1348 2899 4639 5732 1348
Size: 1551 1740 1093 4380
Fragments arranged by size:
4380 1740 1551 1093
PciI A'CATG_T
Cuts at: 5061 7663 5061
Size: 2602 6162
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2520 3063 4915 1108
Size: 1412 543 1852 4957
Fragments arranged by size:
4957 1852 1412 543
PfoI T'CCnGG_A
Cuts at: 5789 5943 5789
Size: 154 8610
PmeI GTTT'AAAC
Cuts at: 6068 6068
Size: 8764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6833 6865 8346 8378 6833
Size: 32 1481 32 7219
Fragments arranged by size:
7219 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4631 5947 4631
Size: 1316 7448
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8764
PsiI TTA'TAA
Cuts at: 3357 6334 3357
Size: 2977 5787
PspOMI G'GGCC_C
Cuts at: 5912 5972 5912
Size: 60 8704
PspXI vC'TCGA_Gb
Cuts at: 2883 2883
Size: 8764
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5753 5785 5753
Size: 32 8732
PstI C_TGCA'G
Cuts at: 830 3085 3802 4677 6116 830
Size: 2255 717 875 1439 3478
Fragments arranged by size:
3478 2255 1439 875 717
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8764
PvuII CAG'CTG
Cuts at: 2534 4234 2534
Size: 1700 7064
SacI G_AGCT'C
Cuts at: 5636 5853 5636
Size: 217 8547
SacII CC_GC'GG
Cuts at: 2615 4736 4806 5396 2615
Size: 2121 70 590 5983
Fragments arranged by size:
5983 2121 590 70
SalI G'TCGA_C
Cuts at: 1936 6106 1936
Size: 4170 4594
SapI GCTCTTCn'nnn_
Cuts at: 2487 2487
Size: 8764
SbfI CC_TGCA'GG
Cuts at: 6116 6116
Size: 8764
ScaI AGT'ACT
Cuts at: 7012 8724 7012
Size: 1712 7052
SfcI C'TryA_G
Cuts at: 826 1045 2599 3081 3798 4159 4242 4673 5415
Size: 219 1554 482 717 361 83 431 742
Cuts at: 5415 5524 6112 7247 7928 8119 826
Size: 109 588 1135 681 191 1471
Fragments arranged by size:
1554 1471 1135 742 717 681 588 482
431 361 219 191 109 83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8764
SmaI CCC'GGG
Cuts at: 1872 2036 3491 6091 1872
Size: 164 1455 2600 4545
Fragments arranged by size:
4545 2600 1455 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2543 2883 3259 3395 3518 5196
Size: 197 926 600 340 376 136 123 1678
Cuts at: 5196 5220 6868 7769 8031 820
Size: 24 1648 901 262 1553
Fragments arranged by size:
1678 1648 1553 926 901 600 376 340
262 197 136 123 24
SnaBI TAC'GTA
Cuts at: 493 6555 493
Size: 6062 2702
SpeI A'CTAG_T
Cuts at: 152 2621 152
Size: 2469 6295
SphI G_CATG'C
Cuts at: 1132 6122 1132
Size: 4990 3774
SspI AAT'ATT
Cuts at: 5 52 6688 5
Size: 47 6636 2081
Fragments arranged by size:
6636 2081 47
StuI AGG'CCT
Cuts at: 1517 3804 1517
Size: 2287 6477
StyI C'CwwG_G
Cuts at: 513 1065 2121 3662 3805 3978 4383 4836 5316
Size: 552 1056 1541 143 173 405 453 480
Cuts at: 5316 5782 5967 6203 513
Size: 466 185 236 3074
Fragments arranged by size:
3074 1541 1056 552 480 466 453 405
236 185 173 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2697 3070 3675 5686 6791 6808 6961
Size: 1255 1117 373 605 2011 1105 17 153
Cuts at: 6961 7146 7591 325
Size: 185 445 1498
Fragments arranged by size:
2011 1498 1255 1117 1105 605 445 373
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5655 7010 8722
Size: 274 80 33 51 1443 3678 1355 1712
Cuts at: 8722 96
Size: 138
Fragments arranged by size:
3678 1712 1443 1355 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2379 2411 5570 5602 8724 8756 2379
Size: 32 3159 32 3122 32 2387
Fragments arranged by size:
3159 3122 2387 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4268 1316
Size: 2952 5812
XbaI T'CTAG_A
Cuts at: 6100 6100
Size: 8764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2683 2803 4624 5833 2683
Size: 120 1821 1209 5614
Fragments arranged by size:
5614 1821 1209 120
XhoI C'TCGA_G
Cuts at: 1943 2883 5196 5220 1943
Size: 940 2313 24 5487
Fragments arranged by size:
5487 2313 940 24
XmaI C'CCGG_G
Cuts at: 1870 2034 3489 6089 1870
Size: 164 1455 2600 4545
Fragments arranged by size:
4545 2600 1455 164
XmnI GAAnn'nnTTC
Cuts at: 2072 4144 6893 2072
Size: 2072 2749 3943
Fragments arranged by size:
3943 2749 2072
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4469 6572 276
Size: 53 83 186 936 2935 2103 2468
Fragments arranged by size:
2935 2468 2103 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI RsrII SanDI
SexAI SfiI SfoI SrfI SwaI