Restriction map for
pFN21AE3446
(Circular) (Six-base) MAPSORT of: pFN21AE3446.seq Check: 910 from: 1 to: 5866
pFN21AE3446 5866 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3676 278
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2373 3209 1937
Size: 436 836 4594
Fragments arranged by size:
4594 836 436
Acc65I G'GTAC_C
Cuts at: 3187 3187
Size: 5866
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2513 2527 3929 5313 5581 1632
Size: 881 14 1402 1384 268 1917
Fragments arranged by size:
1917 1402 1384 881 268 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5669
AflIII A'CryG_T
Cuts at: 1203 4765 5742 1203
Size: 3562 977 1327
Fragments arranged by size:
3562 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 4595 4595
Size: 5866
AleI CACnn'nnGTG
Cuts at: 1166 2363 1166
Size: 1197 4669
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2548 1184
Size: 1364 4502
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2405 2437 2813 2845 2405
Size: 32 376 32 5426
Fragments arranged by size:
5426 376 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2219 2550 3609 5181 2219
Size: 331 1059 1572 2904
Fragments arranged by size:
2904 1572 1059 331
ApaI G_GGCC'C
Cuts at: 3083 3083
Size: 5866
ApaLI G'TGCA_C
Cuts at: 3923 5079 3923
Size: 1156 4710
ApoI r'AATT_y
Cuts at: 1493 3175 3401 3553 5662 1493
Size: 1682 226 152 2109 1697
Fragments arranged by size:
2109 1697 1682 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 3191 1870
Size: 73 1248 4545
Fragments arranged by size:
4545 1248 73
BamHI G'GATC_C
Cuts at: 2757 3196 3563 2757
Size: 439 367 5060
Fragments arranged by size:
5060 439 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2013 3187 4642 618
Size: 325 217 627 226 1174 1455 1842
Fragments arranged by size:
1842 1455 1174 627 325 226 217
BanII G_rGCy'C
Cuts at: 1391 2997 3068 3083 3185 1391
Size: 1606 71 15 102 4072
Fragments arranged by size:
4072 1606 102 71 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4895
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2971 3005 4057 4091 1903
Size: 34 1034 34 1052 34 3678
Fragments arranged by size:
3678 1052 1034 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3758 4968 3758
Size: 1210 4656
BclI T'GATC_A
Cuts at: 1548 2232 2928 1548
Size: 684 696 4486
Fragments arranged by size:
4486 696 684
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4477 136
Size: 107 122 71 631 605 2805 1525
Fragments arranged by size:
2805 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2418 5861 2418
Size: 3443 2423
BlpI GC'TnA_GC
Cuts at: 2626 3283 2626
Size: 657 5209
Bme1580I G_kGCm'C
Cuts at: 1790 2792 3083 3927 5083 1790
Size: 1002 291 844 1156 2573
Fragments arranged by size:
2573 1156 1002 844 291
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5866
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3597 4555 442
Size: 416 53 83 399 24 2180 958 1753
Fragments arranged by size:
2180 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2609 2662 2878 2927 3012 3158 4526
Size: 893 477 53 216 49 85 146 1368
Cuts at: 4526 1239
Size: 2579
Fragments arranged by size:
2579 1368 893 477 216 146 85 53
49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 3991 4856 5154 2156
Size: 1835 865 298 2868
Fragments arranged by size:
2868 1835 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4529 882
Size: 617 3030 2219
Fragments arranged by size:
3030 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3657 493
Size: 711 2453 2702
Fragments arranged by size:
2702 2453 711
BsaBI GATnn'nnATC
Cuts at: 2762 5860 2762
Size: 3098 2768
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3673 4055 4690 275
Size: 53 83 186 936 2140 382 635 1451
Fragments arranged by size:
2140 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2562 3050 4299 4697 4971 5118 5529 2562
Size: 488 1249 398 274 147 411 2899
Fragments arranged by size:
2899 1249 488 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2405 2435 2876 2903 2906
Size: 30 165 30 1002 30 441 27 3
Cuts at: 2906 2933 3131 3161 1178
Size: 27 198 30 3883
Fragments arranged by size:
3883 1002 441 198 165 30 30 30
30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5866
BseYI C'CCAG_C
Cuts at: 2741 5069 5565 2741
Size: 2328 496 3042
Fragments arranged by size:
3042 2328 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2762 1163
Size: 573 1026 4267
Fragments arranged by size:
4267 1026 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 4077 5105 1540
Size: 275 186 1315 761 1028 2301
Fragments arranged by size:
2301 1315 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2316 2997 3927 4012 5083 2316
Size: 681 930 85 1071 3099
Fragments arranged by size:
3099 1071 930 681 85
BsmI GAATG_Cn'
Cuts at: 3377 3470 3377
Size: 93 5773
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2316 2792 2997 3068 3083 3185 3927
Size: 399 526 476 205 71 15 102 742
Cuts at: 3927 4012 5083 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 476 399 205 102
85 71 15
BspEI T'CCGG_A
Cuts at: 5529 5529
Size: 5866
BspHI T'CATG_A
Cuts at: 3755 5485 3755
Size: 1730 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3221 844
Size: 2377 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2814 3753 1394
Size: 429 991 939 3507
Fragments arranged by size:
3507 991 939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2079 2143 4361 4535 65
Size: 1183 831 64 2218 174 1396
Fragments arranged by size:
2218 1396 1183 831 174 64
BsrFI r'CCGG_y
Cuts at: 1700 1810 2098 2304 2667 4510 4697 1700
Size: 110 288 206 363 1843 187 2869
Fragments arranged by size:
2869 1843 363 288 206 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5866
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5866
BssSI C'ACGA_G
Cuts at: 2983 3926 4938 2983
Size: 943 1012 3911
Fragments arranged by size:
3911 1012 943
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2020 794
Size: 1226 4640
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5866
BstYI r'GATC_y
Cuts at: 1972 2418 2757 3196 3563 3948 3965 5417 5503
Size: 446 339 439 367 385 17 1452 86
Cuts at: 5503 5515 5861 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 446 439 385 367 346 339
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2877 3059 513
Size: 552 1812 182 3320
Fragments arranged by size:
3320 1812 552 182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5127
Fragments arranged by size:
5127 683 56
BtsI GCAGTG_nn'
Cuts at: 3382 4176 4196 3382
Size: 794 20 5052
Fragments arranged by size:
5052 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 3170 3170
Size: 5866
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4873 809
Size: 507 3557 1802
Fragments arranged by size:
3557 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2712 3042 3313
Size: 54 1425 50 275 18 882 330 271
Cuts at: 3313 4202 5831 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 882 330 275 271 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3313 1537
Size: 275 1501 4090
Fragments arranged by size:
4090 1501 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2321 3796 1353
Size: 968 1475 3423
Fragments arranged by size:
3423 1475 968
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4453 4827 4973 387
Size: 1064 138 2864 374 146 1280
Fragments arranged by size:
2864 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2995 2995
Size: 5866
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2513 2527 2609 2662 2878 2927
Size: 393 500 381 14 82 53 216 49
Cuts at: 2927 3012 3158 3929 4526 5313 5581 1239
Size: 85 146 771 597 787 268 1524
Fragments arranged by size:
1524 787 771 597 500 393 381 268
216 146 85 82 53 49 14
EcoO109I rG'GnC_Cy
Cuts at: 3004 3004
Size: 5866
EcoRI G'AATT_C
Cuts at: 3175 3553 3175
Size: 378 5488
FspI TGC'GCA
Cuts at: 4372 4372
Size: 5866
HaeII r_GCGC'y
Cuts at: 1678 3027 5013 1678
Size: 1349 1986 2531
Fragments arranged by size:
2531 1986 1349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4507 4539 4581 4613 1373
Size: 32 135 32 2935 32 42 32 2626
Fragments arranged by size:
2935 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3210 3456 669
Size: 988 281 1272 246 3079
Fragments arranged by size:
3079 1272 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5866
HpaI GTT'AAC
Cuts at: 3456 3456
Size: 5866
KpnI G_GTAC'C
Cuts at: 3191 3191
Size: 5866
MfeI C'AATT_G
Cuts at: 2425 3465 2425
Size: 1040 4826
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4980 5164 1305
Size: 3675 184 2007
Fragments arranged by size:
3675 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2714 3044 10
Size: 54 1425 343 882 330 2832
Fragments arranged by size:
2832 1425 882 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 2705 2708 2855 3824 4183 4342
Size: 648 1197 342 3 147 969 359 159
Cuts at: 4342 518
Size: 2042
Fragments arranged by size:
2042 1197 969 648 359 342 159 147
3
MspA1I CmG'CkG
Cuts at: 1606 2937 3282 3959 5107 5352 1606
Size: 1331 345 677 1148 245 2120
Fragments arranged by size:
2120 1331 1148 677 345 245
NaeI GCC'GGC
Cuts at: 2306 2306
Size: 5866
NcoI C'CATG_G
Cuts at: 513 1065 3059 513
Size: 552 1994 3320
Fragments arranged by size:
3320 1994 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5866
NgoMIV G'CCGG_C
Cuts at: 2304 2304
Size: 5866
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NspI r_CATG'y
Cuts at: 1132 1229 2675 2869 3224 4769 1132
Size: 97 1446 194 355 1545 2229
Fragments arranged by size:
2229 1545 1446 355 194 97
PasI CC'CwG_GG
Cuts at: 1348 2605 1348
Size: 1257 4609
PciI A'CATG_T
Cuts at: 4765 4765
Size: 5866
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5866
PfoI T'CCnGG_A
Cuts at: 2177 2586 2177
Size: 409 5457
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2405 2437 3935 3967 5448 5480 2405
Size: 32 1498 32 1481 32 2791
Fragments arranged by size:
2791 1498 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3004 3004
Size: 5866
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5866
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PspOMI G'GGCC_C
Cuts at: 3079 3079
Size: 5866
PstI C_TGCA'G
Cuts at: 830 2025 3218 830
Size: 1195 1193 3478
Fragments arranged by size:
3478 1195 1193
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
SacI G_AGCT'C
Cuts at: 2997 2997
Size: 5866
SalI G'TCGA_C
Cuts at: 1936 3208 1936
Size: 1272 4594
SanDI GG'GwC_CC
Cuts at: 3004 3004
Size: 5866
SapI GCTCTTCn'nnn_
Cuts at: 2321 2321
Size: 5866
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 4114 5826 4114
Size: 1712 4154
SfcI C'TryA_G
Cuts at: 826 1045 2021 3214 4349 5030 5221 826
Size: 219 976 1193 1135 681 191 1471
Fragments arranged by size:
1471 1193 1135 976 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 2098 1700
Size: 398 5468
SmaI CCC'GGG
Cuts at: 1872 3193 1872
Size: 1321 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2171 3970 4871 5133 820
Size: 197 926 228 1799 901 262 1553
Fragments arranged by size:
1799 1553 926 901 262 228 197
SnaBI TAC'GTA
Cuts at: 493 3657 493
Size: 3164 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 2675 2869 3224 1132
Size: 1543 194 355 3774
Fragments arranged by size:
3774 1543 355 194
SspI AAT'ATT
Cuts at: 5 52 3790 5
Size: 47 3738 2081
Fragments arranged by size:
3738 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5866
StyI C'CwwG_G
Cuts at: 513 1065 2085 3059 3305 513
Size: 552 1020 974 246 3074
Fragments arranged by size:
3074 1020 974 552 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3893 3910 4063 4248 4693 325
Size: 1255 2313 17 153 185 445 1498
Fragments arranged by size:
2313 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2883 4112 5824
Size: 274 80 33 51 1443 906 1229 1712
Cuts at: 5824 96
Size: 138
Fragments arranged by size:
1712 1443 1229 906 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2159 2191 5826 5858 2159
Size: 32 3635 32 2167
Fragments arranged by size:
3635 2167 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3114 1316
Size: 1798 4068
XbaI T'CTAG_A
Cuts at: 3202 3202
Size: 5866
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5866
XmaI C'CCGG_G
Cuts at: 1870 3191 1870
Size: 1321 4545
XmnI GAAnn'nnTTC
Cuts at: 3995 3995
Size: 5866
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3674 276
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII
SexAI SfiI SfoI SrfI SwaI XcmI