Restriction map for
pFN21AE3442
(Circular) (Six-base) MAPSORT of: pFN21AE3442.seq Check: 6869 from: 1 to: 8668
pFN21AE3442 8668 bp
With 182 enzymes: SgfI *
October 21, 2016 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3496 3496
Size: 8668
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6478 278
Size: 53 83 186 936 4942 2468
Fragments arranged by size:
4942 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6011 1937
Size: 4074 4594
Acc65I G'GTAC_C
Cuts at: 4897 5592 5989 4897
Size: 695 397 7576
Fragments arranged by size:
7576 695 397
AclI AA'CG_TT
Cuts at: 1563 6795 7168 1563
Size: 5232 373 3063
Fragments arranged by size:
5232 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2681 3393 3719 4109 4213 5929 6731 8115
Size: 1049 712 326 390 104 1716 802 1384
Cuts at: 8115 8383 1632
Size: 268 1917
Fragments arranged by size:
1917 1716 1384 1049 802 712 390 326
268 104
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8471
AflIII A'CryG_T
Cuts at: 1203 5897 7567 8544 1203
Size: 4694 1670 977 1327
Fragments arranged by size:
4694 1670 1327 977
AgeI A'CCGG_T
Cuts at: 5393 7499 5393
Size: 2106 6562
AhdI GACnn_n'nnGTC
Cuts at: 7397 7397
Size: 8668
AleI CACnn'nnGTG
Cuts at: 1166 3129 1166
Size: 1963 6705
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4402 4422 4617 1184
Size: 3218 20 195 5235
Fragments arranged by size:
5235 3218 195 20
AlwNI CAG_nnn'CTG
Cuts at: 2659 2815 3094 3260 3694 3697 4855 5056 5188
Size: 156 279 166 434 3 1158 201 132
Cuts at: 5188 6411 7983 2659
Size: 1223 1572 3344
Fragments arranged by size:
3344 1572 1223 1158 434 279 201 166
156 132 3
ApaI G_GGCC'C
Cuts at: 2070 2730 2824 3638 5638 2070
Size: 660 94 814 2000 5100
Fragments arranged by size:
5100 2000 814 660 94
ApaLI G'TGCA_C
Cuts at: 4806 6725 7881 4806
Size: 1919 1156 5593
Fragments arranged by size:
5593 1919 1156
ApoI r'AATT_y
Cuts at: 1493 2581 3337 4068 4083 5977 6203 6355 8464
Size: 1088 756 731 15 1894 226 152 2109
Cuts at: 8464 1493
Size: 1697
Fragments arranged by size:
2109 1894 1697 1088 756 731 226 152
15
AseI AT'TA_AT
Cuts at: 160 1032 7222 160
Size: 872 6190 1606
Fragments arranged by size:
6190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8668
AvaI C'yCGr_G
Cuts at: 1870 1943 3207 4701 5588 5631 5993 1870
Size: 73 1264 1494 887 43 362 4545
Fragments arranged by size:
4545 1494 1264 887 362 73 43
BamHI G'GATC_C
Cuts at: 5998 6365 5998
Size: 367 8301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2780 4575 4897 5503 5592
Size: 325 217 627 993 1795 322 606 89
Cuts at: 5592 5989 7444 618
Size: 397 1455 1842
Fragments arranged by size:
1842 1795 1455 993 627 606 397 325
322 217 89
BanII G_rGCy'C
Cuts at: 1391 2070 2730 2824 3214 3638 3779 3815 5589
Size: 679 660 94 390 424 141 36 1774
Cuts at: 5589 5638 5987 1391
Size: 49 349 4072
Fragments arranged by size:
4072 1774 679 660 424 390 349 141
94 49 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2669 4028 5760 928
Size: 971 770 1359 1732 3836
Fragments arranged by size:
3836 1732 1359 971 770
BbvCI CC'TCA_GC
Cuts at: 4107 4565 4107
Size: 458 8210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6859 6893 1903
Size: 34 4922 34 3678
Fragments arranged by size:
4922 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5448 6560 7770 5448
Size: 1112 1210 6346
Fragments arranged by size:
6346 1210 1112
BclI T'GATC_A
Cuts at: 1548 3121 3294 1548
Size: 1573 173 6922
Fragments arranged by size:
6922 1573 173
BfrBI ATG'CAT
Cuts at: 5275 5275
Size: 8668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3642 7279
Size: 107 122 71 631 605 337 1633 3637
Cuts at: 7279 136
Size: 1525
Fragments arranged by size:
3637 1633 1525 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2934 3448 8663 2934
Size: 514 5215 2939
Fragments arranged by size:
5215 2939 514
BlpI GC'TnA_GC
Cuts at: 4457 5918 6085 4457
Size: 1461 167 7040
Fragments arranged by size:
7040 1461 167
Bme1580I G_kGCm'C
Cuts at: 1790 2070 2730 2785 2824 3638 4578 4810 5206
Size: 280 660 55 39 814 940 232 396
Cuts at: 5206 5508 5638 6729 7885 1790
Size: 302 130 1091 1156 2573
Fragments arranged by size:
2573 1156 1091 940 814 660 396 302
280 232 130 55 39
BmgBI CAC'GTC
Cuts at: 1318 2501 4836 1318
Size: 1183 2335 5150
Fragments arranged by size:
5150 2335 1183
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2064 4233 4297
Size: 416 53 83 399 24 647 2169 64
Cuts at: 4297 4509 4797 6399 7357 442
Size: 212 288 1602 958 1753
Fragments arranged by size:
2169 1753 1602 958 647 416 399 288
212 83 64 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8668
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 3793 3877 5112 5317 2777
Size: 1016 84 1235 205 6128
Fragments arranged by size:
6128 1235 1016 205 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2764 3498 4037 4058 4154 4471 5535 5624
Size: 1525 734 539 21 96 317 1064 89
Cuts at: 5624 7328 1239
Size: 1704 2579
Fragments arranged by size:
2579 1704 1525 1064 734 539 317 96
89 21
Bpu10I CC'TnA_GC
Cuts at: 4107 4565 4107
Size: 458 8210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3596 5407 6793 7658 7956 3596
Size: 1811 1386 865 298 4308
Fragments arranged by size:
4308 1811 1386 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7331 882
Size: 617 5832 2219
Fragments arranged by size:
5832 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2767 5898 6459 493
Size: 711 1563 3131 561 2702
Fragments arranged by size:
3131 2702 1563 711 561
BsaBI GATnn'nnATC
Cuts at: 2598 8662 2598
Size: 6064 2604
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2967 6475 6857 7492
Size: 53 83 186 936 1434 3508 382 635
Cuts at: 7492 275
Size: 1451
Fragments arranged by size:
3508 1451 1434 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5393 7101 7499 7773 7920 8331 5393
Size: 1708 398 274 147 411 5730
Fragments arranged by size:
5730 1708 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3812 3842 4783 4813 1178
Size: 30 165 30 2409 30 941 30 5033
Fragments arranged by size:
5033 2409 941 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3415 3678 3785 4095 4356 5306 5869 1186
Size: 2229 263 107 310 261 950 563 3985
Fragments arranged by size:
3985 2229 950 563 310 263 261 107
BseYI C'CCAG_C
Cuts at: 2107 2155 2304 3687 4026 4712 5099 5181 5507
Size: 48 149 1383 339 686 387 82 326
Cuts at: 5507 5596 5717 7871 8367 2107
Size: 89 121 2154 496 2408
Fragments arranged by size:
2408 2154 1383 686 496 387 339 326
149 121 89 82 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2676 3308 3824 3827 4632 1163
Size: 573 940 632 516 3 805 5199
Fragments arranged by size:
5199 940 805 632 573 516 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2139 2212 6118 6879 7907 1540
Size: 275 186 138 73 3906 761 1028 2301
Fragments arranged by size:
3906 2301 1028 761 275 186 138 73
BsiHKAI G_wGCw'C
Cuts at: 3193 3779 3815 4810 5622 5844 6729 6814 7885
Size: 586 36 995 812 222 885 85 1071
Cuts at: 7885 3193
Size: 3976
Fragments arranged by size:
3976 1071 995 885 812 586 222 85
36
BsmI GAATG_Cn'
Cuts at: 3005 4933 5275 5486 6179 6272 3005
Size: 1928 342 211 693 93 5401
Fragments arranged by size:
5401 1928 693 342 211 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3188 3188
Size: 8668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2730 2785 2824 3193 3214 3638
Size: 399 280 660 55 39 369 21 424
Cuts at: 3638 3779 3815 4578 4810 5206 5508 5589 5622
Size: 141 36 763 232 396 302 81 33
Cuts at: 5622 5638 5844 5987 6729 6814 7885 1391
Size: 16 206 143 742 85 1071 2174
Fragments arranged by size:
2174 1071 763 742 660 424 399 396
369 302 280 232 206 143 141 85
81 55 39 36 33 21 16
BspEI T'CCGG_A
Cuts at: 8331 8331
Size: 8668
BspHI T'CATG_A
Cuts at: 5836 6557 8287 5836
Size: 721 1730 6217
Fragments arranged by size:
6217 1730 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3245 3496 4394 4411 4885 6023 844
Size: 2401 251 898 17 474 1138 3489
Fragments arranged by size:
3489 2401 1138 898 474 251 17
BsrBI CCG'CTC
Cuts at: 1394 1823 2025 6555 1394
Size: 429 202 4530 3507
Fragments arranged by size:
4530 3507 429 202
BsrDI GCAATG_nn'
Cuts at: 65 1248 7163 7337 65
Size: 1183 5915 174 1396
Fragments arranged by size:
5915 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2138 3873 4943 5263 5393 7312 7499
Size: 110 328 1735 1070 320 130 1919 187
Cuts at: 7499 1700
Size: 2869
Fragments arranged by size:
2869 1919 1735 1070 328 320 187 130
110
BsrGI T'GTAC_A
Cuts at: 96 5680 96
Size: 5584 3084
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8668
BssSI C'ACGA_G
Cuts at: 5302 5711 6728 7740 5302
Size: 409 1017 1012 6230
Fragments arranged by size:
6230 1017 1012 409
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2815 3260 3556 4855 794
Size: 2021 445 296 1299 4607
Fragments arranged by size:
4607 2021 1299 445 296
BstBI TT'CG_AA
Cuts at: 7472 7472
Size: 8668
BstEII G'GTnAC_C
Cuts at: 1184 4423 1184
Size: 3239 5429
BstXI CCAn_nnnn'nTGG
Cuts at: 4555 4555
Size: 8668
BstYI r'GATC_y
Cuts at: 1972 2905 2934 3448 5998 6365 6750 6767 8219
Size: 933 29 514 2550 367 385 17 1452
Cuts at: 8219 8305 8317 8663 1972
Size: 86 12 346 1977
Fragments arranged by size:
2550 1977 1452 933 514 385 367 346
86 29 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2170 2473 2925 3060 3707
Size: 552 939 66 100 303 452 135 647
Cuts at: 3707 5245 513
Size: 1538 3936
Fragments arranged by size:
3936 1538 939 647 552 452 303 135
100 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7929
Fragments arranged by size:
7929 683 56
BtsI GCAGTG_nn'
Cuts at: 2804 3092 3750 4270 4333 5568 6184 6978 6998
Size: 288 658 520 63 1235 616 794 20
Cuts at: 6998 2804
Size: 4474
Fragments arranged by size:
4474 1235 794 658 616 520 288 63
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4719 4754 529
Size: 35 4155 35 4443
Fragments arranged by size:
4443 4155 35 35
DraI TTT'AAA
Cuts at: 2571 5874 5972 2571
Size: 3303 98 5267
Fragments arranged by size:
5267 3303 98
DraIII CAC_nnn'GTG
Cuts at: 2467 3412 2467
Size: 945 7723
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3097 7675 809
Size: 507 1781 4578 1802
Fragments arranged by size:
4578 1802 1781 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2140 3057
Size: 54 1425 50 275 18 306 4 917
Cuts at: 3057 3643 4557 4945 6115 7004 8633 8
Size: 586 914 388 1170 889 1629 43
Fragments arranged by size:
1629 1425 1170 917 914 889 586 388
306 275 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2136 6115 1537
Size: 275 324 3979 4090
Fragments arranged by size:
4090 3979 324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2645 3354 4128 4232 6598 1353
Size: 1292 709 774 104 2366 3423
Fragments arranged by size:
3423 2366 1292 774 709 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2079 2876 3260 7255 7629 7775
Size: 1064 138 490 797 384 3995 374 146
Cuts at: 7775 387
Size: 1280
Fragments arranged by size:
3995 1280 1064 797 490 384 374 146
138
EcoICRI GAG'CTC
Cuts at: 3777 3813 3777
Size: 36 8632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2681 2764 3393 3498 3719 4037 4058
Size: 393 1049 83 629 105 221 318 21
Cuts at: 4058 4109 4154 4213 4471 5535 5624 5929 6731
Size: 51 45 59 258 1064 89 305 802
Cuts at: 6731 7328 8115 8383 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1064 1049 802 787 629 597 393
318 305 268 258 221 105 89 83
59 51 45 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3041 4731 5523 3041
Size: 1690 792 6186
Fragments arranged by size:
6186 1690 792
EcoO109I rG'GnC_Cy
Cuts at: 2103 3635 3713 4852 5407 5628 5635 2103
Size: 1532 78 1139 555 221 7 5136
Fragments arranged by size:
5136 1532 1139 555 221 78 7
EcoRI G'AATT_C
Cuts at: 5977 6355 5977
Size: 378 8290
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2248 4937 5341 2248
Size: 2689 404 5575
Fragments arranged by size:
5575 2689 404
FseI GG_CCGG'CC
Cuts at: 2142 2142
Size: 8668
FspI TGC'GCA
Cuts at: 7174 7174
Size: 8668
HaeII r_GCGC'y
Cuts at: 1678 2920 3706 5131 7815 1678
Size: 1242 786 1425 2684 2531
Fragments arranged by size:
2684 2531 1425 1242 786
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2404 2436 3657 3689 4264
Size: 32 135 32 832 32 1221 32 575
Cuts at: 4264 4296 5399 5431 5505 5537 7309 7341 7383
Size: 32 1103 32 74 32 1772 32 42
Cuts at: 7383 7415 1373
Size: 32 2626
Fragments arranged by size:
2626 1772 1221 1103 832 575 135 74
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3484 4450 6012 6258 669
Size: 988 281 1546 966 1562 246 3079
Fragments arranged by size:
3079 1562 1546 988 966 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8668
HpaI GTT'AAC
Cuts at: 6258 6258
Size: 8668
KpnI G_GTAC'C
Cuts at: 4901 5596 5993 4901
Size: 695 397 7576
Fragments arranged by size:
7576 695 397
MfeI C'AATT_G
Cuts at: 6267 6267
Size: 8668
MluI A'CGCG_T
Cuts at: 8544 8544
Size: 8668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2483 2528 3700 3855 4775 5727 7782 7966
Size: 1178 45 1172 155 920 952 2055 184
Cuts at: 7966 1305
Size: 2007
Fragments arranged by size:
2055 2007 1178 1172 952 920 184 155
45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3059 4559 10
Size: 54 1425 343 1227 1500 4119
Fragments arranged by size:
4119 1500 1425 1227 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3129 5209 5250 5463 5896 6626 6985
Size: 648 1963 2080 41 213 433 730 359
Cuts at: 6985 7144 518
Size: 159 2042
Fragments arranged by size:
2080 2042 1963 730 648 433 359 213
159 41
MspA1I CmG'CkG
Cuts at: 1606 2072 2120 2172 2308 2528 2656 3292 3619
Size: 466 48 52 136 220 128 636 327
Cuts at: 3619 3694 3955 3976 4478 4597 5686 6084 6761
Size: 75 261 21 502 119 1089 398 677
Cuts at: 6761 7909 8154 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1089 677 636 502 466 398
327 261 245 220 136 128 119 75
52 48 21
NaeI GCC'GGC
Cuts at: 2140 5265 2140
Size: 3125 5543
NcoI C'CATG_G
Cuts at: 513 1065 2004 2473 2925 3060 513
Size: 552 939 469 452 135 6121
Fragments arranged by size:
6121 939 552 469 452 135
NdeI CA'TA_TG
Cuts at: 387 2892 4038 387
Size: 2505 1146 5017
Fragments arranged by size:
5017 2505 1146
NgoMIV G'CCGG_C
Cuts at: 2138 5263 2138
Size: 3125 5543
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8668
NotI GC'GGCC_GC
Cuts at: 6115 6115
Size: 8668
NsiI A_TGCA'T
Cuts at: 5277 5277
Size: 8668
NspI r_CATG'y
Cuts at: 1132 1229 3205 3686 4137 4456 4468 6026 7571
Size: 97 1976 481 451 319 12 1558 1545
Cuts at: 7571 1132
Size: 2229
Fragments arranged by size:
2229 1976 1558 1545 481 451 319 97
12
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8668
PciI A'CATG_T
Cuts at: 7567 7567
Size: 8668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2398 2467 2795 2896 3316 5449 1108
Size: 1290 69 328 101 420 2133 4327
Fragments arranged by size:
4327 2133 1290 420 328 101 69
PfoI T'CCnGG_A
Cuts at: 2337 3475 5551 2337
Size: 1138 2076 5454
Fragments arranged by size:
5454 2076 1138
PmeI GTTT'AAAC
Cuts at: 5972 5972
Size: 8668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 6737 6769 8250 8282 4346
Size: 32 2359 32 1481 32 4732
Fragments arranged by size:
4732 2359 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2103 3713 5407 2103
Size: 1610 1694 5364
Fragments arranged by size:
5364 1694 1610
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8668
PsiI TTA'TAA
Cuts at: 6238 6238
Size: 8668
PspOMI G'GGCC_C
Cuts at: 2066 2726 2820 3634 5634 2066
Size: 660 94 814 2000 5100
Fragments arranged by size:
5100 2000 814 660 94
PstI C_TGCA'G
Cuts at: 830 2811 3099 3456 3507 3960 3981 4483 6020
Size: 1981 288 357 51 453 21 502 1537
Cuts at: 6020 830
Size: 3478
Fragments arranged by size:
3478 1981 1537 502 453 357 288 51
21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8668
PvuII CAG'CTG
Cuts at: 2308 2528 2656 3292 3619 3694 3955 3976 4478
Size: 220 128 636 327 75 261 21 502
Cuts at: 4478 4597 5686 2308
Size: 119 1089 5290
Fragments arranged by size:
5290 1089 636 502 327 261 220 128
119 75 21
SacI G_AGCT'C
Cuts at: 3779 3815 3779
Size: 36 8632
SacII CC_GC'GG
Cuts at: 2073 2173 2073
Size: 100 8568
SalI G'TCGA_C
Cuts at: 1936 6010 1936
Size: 4074 4594
SanDI GG'GwC_CC
Cuts at: 2103 3713 5407 2103
Size: 1610 1694 5364
Fragments arranged by size:
5364 1694 1610
SapI GCTCTTCn'nnn_
Cuts at: 3354 3354
Size: 8668
SbfI CC_TGCA'GG
Cuts at: 3507 6020 3507
Size: 2513 6155
ScaI AGT'ACT
Cuts at: 6916 8628 6916
Size: 1712 6956
SexAI A'CCwGG_T
Cuts at: 2391 2463 2666 2391
Size: 72 203 8393
Fragments arranged by size:
8393 203 72
SfcI C'TryA_G
Cuts at: 826 1045 2807 3095 3127 3139 3452 3503 3599
Size: 219 1762 288 32 12 313 51 96
Cuts at: 3599 3614 3956 3977 4479 4720 4844 6016 7151
Size: 15 342 21 502 241 124 1172 1135
Cuts at: 7151 7832 8023 826
Size: 681 191 1471
Fragments arranged by size:
1762 1471 1172 1135 681 502 342 313
288 241 219 191 124 96 51 32
21 15 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 3642 3642
Size: 8668
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8668
SmaI CCC'GGG
Cuts at: 1872 4703 5633 5995 1872
Size: 2831 930 362 4545
Fragments arranged by size:
4545 2831 930 362
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3575 5386 6772 7673 7935 820
Size: 197 926 1632 1811 1386 901 262 1553
Fragments arranged by size:
1811 1632 1553 1386 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6459 493
Size: 5966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8668
SphI G_CATG'C
Cuts at: 1132 3205 3686 6026 1132
Size: 2073 481 2340 3774
Fragments arranged by size:
3774 2340 2073 481
SspI AAT'ATT
Cuts at: 5 52 2454 6592 5
Size: 47 2402 4138 2081
Fragments arranged by size:
4138 2402 2081 47
StuI AGG'CCT
Cuts at: 1517 3905 1517
Size: 2388 6280
StyI C'CwwG_G
Cuts at: 513 1065 2004 2268 2274 2473 2749 2925 3060
Size: 552 939 264 6 199 276 176 135
Cuts at: 3060 3640 5074 5104 5316 6107 513
Size: 580 1434 30 212 791 3074
Fragments arranged by size:
3074 1434 939 791 580 552 276 264
212 199 176 135 30 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3404 5248 5859 6695 6712 6865 7050
Size: 1255 1824 1844 611 836 17 153 185
Cuts at: 7050 7495 325
Size: 445 1498
Fragments arranged by size:
1844 1824 1498 1255 836 611 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3579 5348 5680
Size: 274 80 33 51 1443 1602 1769 332
Cuts at: 5680 6914 8626 96
Size: 1234 1712 138
Fragments arranged by size:
1769 1712 1602 1443 1234 332 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4783 4815 5288 5320 5547 5579 8628 8660 4783
Size: 32 473 32 227 32 3049 32 4791
Fragments arranged by size:
4791 3049 473 227 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8668
XbaI T'CTAG_A
Cuts at: 6004 6004
Size: 8668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3067 3067
Size: 8668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8668
XmaI C'CCGG_G
Cuts at: 1870 4701 5631 5993 1870
Size: 2831 930 362 4545
Fragments arranged by size:
4545 2831 930 362
XmnI GAAnn'nnTTC
Cuts at: 2333 4640 6797 2333
Size: 2307 2157 4204
Fragments arranged by size:
4204 2307 2157
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6476 276
Size: 53 83 186 936 4942 2468
Fragments arranged by size:
4942 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
Bsu36I EcoRV FspAI KasI NarI NruI PacI PmlI
PspXI PsrI RsrII SfoI SrfI SwaI