Restriction map for
pFN21AE3432
(Circular) (Six-base) MAPSORT of: pFN21AE3432.seq Check: 8278 from: 1 to: 7129
pFN21AE3432 7129 bp
With 182 enzymes: SgfI *
March 28, 2012 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4939 278
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3576 3813 4472 1937
Size: 1639 237 659 4594
Fragments arranged by size:
4594 1639 659 237
Acc65I G'GTAC_C
Cuts at: 4450 4450
Size: 7129
AclI AA'CG_TT
Cuts at: 1563 5256 5629 1563
Size: 3693 373 3063
Fragments arranged by size:
3693 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2373 5192 6576 6844 1632
Size: 741 2819 1384 268 1917
Fragments arranged by size:
2819 1917 1384 741 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6932
AflIII A'CryG_T
Cuts at: 1203 3162 6028 7005 1203
Size: 1959 2866 977 1327
Fragments arranged by size:
2866 1959 1327 977
AgeI A'CCGG_T
Cuts at: 5960 5960
Size: 7129
AhdI GACnn_n'nnGTC
Cuts at: 5858 5858
Size: 7129
AleI CACnn'nnGTG
Cuts at: 1166 3221 1166
Size: 2055 5074
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2088 1184
Size: 904 6225
AlwNI CAG_nnn'CTG
Cuts at: 2318 3898 3940 4420 4872 6444 2318
Size: 1580 42 480 452 1572 3003
Fragments arranged by size:
3003 1580 1572 480 452 42
ApaLI G'TGCA_C
Cuts at: 5186 6342 5186
Size: 1156 5973
ApoI r'AATT_y
Cuts at: 1493 2119 2736 3749 3784 4438 4664 4816 6925
Size: 626 617 1013 35 654 226 152 2109
Cuts at: 6925 1493
Size: 1697
Fragments arranged by size:
2109 1697 1013 654 626 617 226 152
35
AseI AT'TA_AT
Cuts at: 160 1032 5683 160
Size: 872 4651 1606
Fragments arranged by size:
4651 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
AvaI C'yCGr_G
Cuts at: 1870 1943 2295 2505 3068 4454 1870
Size: 73 352 210 563 1386 4545
Fragments arranged by size:
4545 1386 563 352 210 73
BamHI G'GATC_C
Cuts at: 2523 2718 2998 3153 3406 4459 4826 2523
Size: 195 280 155 253 1053 367 4826
Fragments arranged by size:
4826 1053 367 280 253 195 155
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2908 3133 3324 3610
Size: 325 217 627 285 836 225 191 286
Cuts at: 3610 4450 5905 618
Size: 840 1455 1842
Fragments arranged by size:
1842 1455 840 836 627 325 286 285
225 217 191
BanII G_rGCy'C
Cuts at: 1391 2177 2393 2758 4448 1391
Size: 786 216 365 1690 4072
Fragments arranged by size:
4072 1690 786 365 216
BbeI G_GCGC'C
Cuts at: 3328 3328
Size: 7129
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2352 3425 928
Size: 971 453 1073 4632
Fragments arranged by size:
4632 1073 971 453
BbvCI CC'TCA_GC
Cuts at: 4332 4332
Size: 7129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2384 2418 5320 5354 1903
Size: 34 447 34 2902 34 3678
Fragments arranged by size:
3678 2902 447 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5021 6231 5021
Size: 1210 5919
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7129
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3875 5740 136
Size: 107 122 71 631 605 2203 1865 1525
Fragments arranged by size:
2203 1865 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7124 7124
Size: 7129
BlpI GC'TnA_GC
Cuts at: 2757 3249 4546 2757
Size: 492 1297 5340
Fragments arranged by size:
5340 1297 492
Bme1580I G_kGCm'C
Cuts at: 1790 3054 3087 3175 3615 4160 5190 6346 1790
Size: 1264 33 88 440 545 1030 1156 2573
Fragments arranged by size:
2573 1264 1156 1030 545 440 88 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7129
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2269 3399 3669
Size: 416 53 83 399 24 852 1130 270
Cuts at: 3669 4086 4860 5818 442
Size: 417 774 958 1753
Fragments arranged by size:
1753 1130 958 852 774 417 416 399
270 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 7129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2676 3091 3198 3446 3997 5789 1239
Size: 1437 415 107 248 551 1792 2579
Fragments arranged by size:
2579 1792 1437 551 415 248 107
Bpu10I CC'TnA_GC
Cuts at: 4332 4332
Size: 7129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2786 3242 5254 6119 6417 2786
Size: 456 2012 865 298 3498
Fragments arranged by size:
3498 2012 865 456 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2537 2781 2952 5792 882
Size: 617 1038 244 171 2840 2219
Fragments arranged by size:
2840 2219 1038 617 244 171
BsaAI yAC'GTr
Cuts at: 493 1204 4920 493
Size: 711 3716 2702
Fragments arranged by size:
3716 2702 711
BsaBI GATnn'nnATC
Cuts at: 7123 7123
Size: 7129
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2953 3240 3325 4936
Size: 53 83 186 936 1420 287 85 1611
Cuts at: 4936 5318 5953 275
Size: 382 635 1451
Fragments arranged by size:
1611 1451 1420 936 635 382 287 186
85 83 53
BsaWI w'CCGG_w
Cuts at: 2928 5562 5960 6234 6381 6792 2928
Size: 2634 398 274 147 411 3265
Fragments arranged by size:
3265 2634 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4333 4363 1178
Size: 30 165 30 2930 30 3944
Fragments arranged by size:
3944 2930 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2179 2283 2351 2354 2465 2555 2570 1186
Size: 993 104 68 3 111 90 15 5745
Fragments arranged by size:
5745 993 111 104 90 68 15 3
BseYI C'CCAG_C
Cuts at: 2311 2519 2663 3080 3201 3286 6332 6828 2311
Size: 208 144 417 121 85 3046 496 2612
Fragments arranged by size:
3046 2612 496 417 208 144 121 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3520 1163
Size: 573 1784 4772
Fragments arranged by size:
4772 1784 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4579 5340 6368 1540
Size: 275 186 2578 761 1028 2301
Fragments arranged by size:
2578 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2146 3457 5190 5275 6346 2146
Size: 1311 1733 85 1071 2929
Fragments arranged by size:
2929 1733 1311 1071 85
BsmI GAATG_Cn'
Cuts at: 4147 4640 4733 4147
Size: 493 93 6543
Fragments arranged by size:
6543 493 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2146 2177 2393 2758 3054 3087 3175
Size: 399 356 31 216 365 296 33 88
Cuts at: 3175 3457 3615 4160 4448 5190 5275 6346 1391
Size: 282 158 545 288 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 545 399 365 356 296
288 282 216 158 88 85 33 31
BspEI T'CCGG_A
Cuts at: 2928 6792 2928
Size: 3864 3265
BspHI T'CATG_A
Cuts at: 5018 6748 5018
Size: 1730 5399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2706 4484 844
Size: 1862 1778 3489
Fragments arranged by size:
3489 1862 1778
BsrBI CCG'CTC
Cuts at: 1394 1823 3151 3253 5016 1394
Size: 429 1328 102 1763 3507
Fragments arranged by size:
3507 1763 1328 429 102
BsrDI GCAATG_nn'
Cuts at: 65 1248 2219 3732 5624 5798 65
Size: 1183 971 1513 1892 174 1396
Fragments arranged by size:
1892 1513 1396 1183 971 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2796 4174 5773 5960 1700
Size: 110 986 1378 1599 187 2869
Fragments arranged by size:
2869 1599 1378 986 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7129
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7129
BssSI C'ACGA_G
Cuts at: 5189 6201 5189
Size: 1012 6117
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3091 3898 4270 794
Size: 2297 807 372 3653
Fragments arranged by size:
3653 2297 807 372
BstBI TT'CG_AA
Cuts at: 5933 5933
Size: 7129
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7129
BstXI CCAn_nnnn'nTGG
Cuts at: 2258 3196 3320 3343 2258
Size: 938 124 23 6044
Fragments arranged by size:
6044 938 124 23
BstYI r'GATC_y
Cuts at: 1972 2523 2718 2809 2998 3153 3406 4459 4826
Size: 551 195 91 189 155 253 1053 367
Cuts at: 4826 5211 5228 6680 6766 6778 7124 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1053 551 385 367 346 253
195 189 155 91 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2208 3822 4133 4218 2208
Size: 1614 311 85 5119
Fragments arranged by size:
5119 1614 311 85
BtgI C'CryG_G
Cuts at: 513 1065 2004 2374 3615 513
Size: 552 939 370 1241 4027
Fragments arranged by size:
4027 1241 939 552 370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6390
Fragments arranged by size:
6390 683 56
BtsI GCAGTG_nn'
Cuts at: 2329 3388 4645 5439 5459 2329
Size: 1059 1257 794 20 3999
Fragments arranged by size:
3999 1257 1059 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7129
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7094
DraI TTT'AAA
Cuts at: 4433 4433
Size: 7129
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2200 2581 6136 809
Size: 507 884 381 3555 1802
Fragments arranged by size:
3555 1802 884 507 381
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2814 3631 4576
Size: 54 1425 50 275 18 984 817 945
Cuts at: 4576 5465 7094 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 984 945 889 817 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4576 1537
Size: 275 2764 4090
Fragments arranged by size:
4090 2764 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2328 2532 2556 2598 2870 3552 5059 1353
Size: 975 204 24 42 272 682 1507 3423
Fragments arranged by size:
3423 1507 975 682 272 204 42 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5716 6090 6236 387
Size: 1064 138 4127 374 146 1280
Fragments arranged by size:
4127 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2373 2676 3091 3198 3446 3997 5192
Size: 393 741 303 415 107 248 551 1195
Cuts at: 5192 5789 6576 6844 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1195 787 741 597 551 415 393
303 268 248 107
EcoNI CCTnn'n_nnAGG
Cuts at: 2583 2966 3464 2583
Size: 383 498 6248
Fragments arranged by size:
6248 498 383
EcoO109I rG'GnC_Cy
Cuts at: 2270 2380 2588 2617 4328 2270
Size: 110 208 29 1711 5071
Fragments arranged by size:
5071 1711 208 110 29
EcoRI G'AATT_C
Cuts at: 2736 4438 4816 2736
Size: 1702 378 5049
Fragments arranged by size:
5049 1702 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3946 3946
Size: 7129
FspI TGC'GCA
Cuts at: 5635 5635
Size: 7129
HaeII r_GCGC'y
Cuts at: 1678 2311 3328 3667 6276 1678
Size: 633 1017 339 2609 2531
Fragments arranged by size:
2609 2531 1017 633 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2423 2455 5770 5802 5844
Size: 32 135 32 851 32 3315 32 42
Cuts at: 5844 5876 1373
Size: 32 2626
Fragments arranged by size:
3315 2626 851 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2992 4473 4719 669
Size: 988 281 1054 1481 246 3079
Fragments arranged by size:
3079 1481 1054 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4005 748
Size: 3257 3872
HpaI GTT'AAC
Cuts at: 2992 4719 2992
Size: 1727 5402
KasI G'GCGC_C
Cuts at: 3324 3324
Size: 7129
KpnI G_GTAC'C
Cuts at: 4454 4454
Size: 7129
MfeI C'AATT_G
Cuts at: 4728 4728
Size: 7129
MluI A'CGCG_T
Cuts at: 7005 7005
Size: 7129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1998 2460 6243 6427 1305
Size: 693 462 3783 184 2007
Fragments arranged by size:
3783 2007 693 462 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2816 3633 10
Size: 54 1425 343 984 817 3506
Fragments arranged by size:
3506 1425 984 817 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3221 3318 5087 5446 5605 518
Size: 648 2055 97 1769 359 159 2042
Fragments arranged by size:
2055 2042 1769 648 359 159 97
MspA1I CmG'CkG
Cuts at: 1606 2258 2761 3205 3898 4040 4417 4545 5222
Size: 652 503 444 693 142 377 128 677
Cuts at: 5222 6370 6615 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 693 677 652 503 444 377
245 142 128
NarI GG'CG_CC
Cuts at: 3325 3325
Size: 7129
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5638
Fragments arranged by size:
5638 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7129
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7129
NotI GC'GGCC_GC
Cuts at: 4576 4576
Size: 7129
NspI r_CATG'y
Cuts at: 1132 1229 3094 3115 3166 4487 6032 1132
Size: 97 1865 21 51 1321 1545 2229
Fragments arranged by size:
2229 1865 1545 1321 97 51 21
PasI CC'CwG_GG
Cuts at: 1348 3004 1348
Size: 1656 5473
PciI A'CATG_T
Cuts at: 3162 6028 3162
Size: 2866 4263
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2225 2318 3718 1108
Size: 1117 93 1400 4519
Fragments arranged by size:
4519 1400 1117 93
PfoI T'CCnGG_A
Cuts at: 2721 3156 4020 2721
Size: 435 864 5830
Fragments arranged by size:
5830 864 435
PmeI GTTT'AAAC
Cuts at: 4433 4433
Size: 7129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5198 5230 6711 6743 5198
Size: 32 1481 32 5584
Fragments arranged by size:
5584 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2270 2380 2270
Size: 110 7019
PshAI GACnn'nnGTC
Cuts at: 1613 2275 1613
Size: 662 6467
PsiI TTA'TAA
Cuts at: 4699 4699
Size: 7129
PspXI vC'TCGA_Gb
Cuts at: 2295 2295
Size: 7129
PstI C_TGCA'G
Cuts at: 830 2107 2625 3099 3383 3903 4481 830
Size: 1277 518 474 284 520 578 3478
Fragments arranged by size:
3478 1277 578 520 518 474 284
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7129
PvuII CAG'CTG
Cuts at: 2258 3205 3898 4040 4417 2258
Size: 947 693 142 377 4970
Fragments arranged by size:
4970 947 693 377 142
SalI G'TCGA_C
Cuts at: 1936 4471 1936
Size: 2535 4594
SanDI GG'GwC_CC
Cuts at: 2270 2270
Size: 7129
SbfI CC_TGCA'GG
Cuts at: 4481 4481
Size: 7129
ScaI AGT'ACT
Cuts at: 3363 5377 7089 3363
Size: 2014 1712 3403
Fragments arranged by size:
3403 2014 1712
SexAI A'CCwGG_T
Cuts at: 3525 3525
Size: 7129
SfcI C'TryA_G
Cuts at: 826 1045 2103 2621 3095 3379 3899 4477 5612
Size: 219 1058 518 474 284 520 578 1135
Cuts at: 5612 6293 6484 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1058 681 578 520 518 474
284 219 191
SfoI GGC'GCC
Cuts at: 3326 3326
Size: 7129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7129
SmaI CCC'GGG
Cuts at: 1872 2507 4456 1872
Size: 635 1949 4545
Fragments arranged by size:
4545 1949 635
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2295 2801 3257 5233 6134 6396
Size: 197 926 352 506 456 1976 901 262
Cuts at: 6396 820
Size: 1553
Fragments arranged by size:
1976 1553 926 901 506 456 352 262
197
SnaBI TAC'GTA
Cuts at: 493 4920 493
Size: 4427 2702
SpeI A'CTAG_T
Cuts at: 152 3028 152
Size: 2876 4253
SphI G_CATG'C
Cuts at: 1132 4487 1132
Size: 3355 3774
SspI AAT'ATT
Cuts at: 5 52 5053 5
Size: 47 5001 2081
Fragments arranged by size:
5001 2081 47
StuI AGG'CCT
Cuts at: 1517 3926 1517
Size: 2409 4720
StyI C'CwwG_G
Cuts at: 513 1065 2004 3129 3336 3402 3870 4152 4237
Size: 552 939 1125 207 66 468 282 85
Cuts at: 4237 4248 4323 4568 513
Size: 11 75 245 3074
Fragments arranged by size:
3074 1125 939 552 468 282 245 207
85 75 66 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2561 3372 3559 5156 5173 5326 5511
Size: 1255 981 811 187 1597 17 153 185
Cuts at: 5511 5956 325
Size: 445 1498
Fragments arranged by size:
1597 1498 1255 981 811 445 187 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3361 5375 7087
Size: 274 80 33 51 1443 1384 2014 1712
Cuts at: 7087 96
Size: 138
Fragments arranged by size:
2014 1712 1443 1384 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3018 3050 4333 4365 7089 7121 3018
Size: 32 1283 32 2724 32 3026
Fragments arranged by size:
3026 2724 1283 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3494 1316
Size: 2178 4951
XbaI T'CTAG_A
Cuts at: 4465 4465
Size: 7129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 3719 3941 2167
Size: 1552 222 5355
Fragments arranged by size:
5355 1552 222
XhoI C'TCGA_G
Cuts at: 1943 2295 1943
Size: 352 6777
XmaI C'CCGG_G
Cuts at: 1870 2505 4454 1870
Size: 635 1949 4545
Fragments arranged by size:
4545 1949 635
XmnI GAAnn'nnTTC
Cuts at: 5258 5258
Size: 7129
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4937 276
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI
RsrII SacI SacII SapI SfiI SrfI SwaI