Restriction map for
pFN21AE3426
(Circular) (Six-base) MAPSORT of: pFN21AE3426.seq Check: 9403 from: 1 to: 6484
pFN21AE3426 6484 bp
With 182 enzymes: SgfI *
March 28, 2012 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3670 3757 3670
Size: 87 6397
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2097 4294 278
Size: 53 83 186 936 561 2197 2468
Fragments arranged by size:
2468 2197 936 561 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3827 1937
Size: 1890 4594
Acc65I G'GTAC_C
Cuts at: 2310 3805 2310
Size: 1495 4989
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4547 5931 6199 1632
Size: 2915 1384 268 1917
Fragments arranged by size:
2915 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 5383 6360 1203
Size: 4180 977 1327
Fragments arranged by size:
4180 1327 977
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 5213 5213
Size: 6484
AleI CACnn'nnGTG
Cuts at: 1166 3236 3513 3515 1166
Size: 2070 277 2 4135
Fragments arranged by size:
4135 2070 277 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6484
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2103 2135 3674 3706 2103
Size: 32 1539 32 4881
Fragments arranged by size:
4881 1539 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4227 5799 4227
Size: 1572 4912
ApaLI G'TGCA_C
Cuts at: 4541 5697 4541
Size: 1156 5328
ApoI r'AATT_y
Cuts at: 1493 2519 3793 4019 4171 6280 1493
Size: 1026 1274 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 1026 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 3647 3809 1870
Size: 73 1704 162 4545
Fragments arranged by size:
4545 1704 162 73
BamHI G'GATC_C
Cuts at: 3814 4181 3814
Size: 367 6117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2293 2310 2319 2368 2473
Size: 325 217 627 506 17 9 49 105
Cuts at: 2473 2744 2803 3097 3805 5260 618
Size: 271 59 294 708 1455 1842
Fragments arranged by size:
1842 1455 708 627 506 325 294 271
217 105 59 49 17 9
BanII G_rGCy'C
Cuts at: 1391 3071 3803 1391
Size: 1680 732 4072
Fragments arranged by size:
4072 1680 732
BbeI G_GCGC'C
Cuts at: 2323 2748 2807 2323
Size: 425 59 6000
Fragments arranged by size:
6000 425 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2974 2998 3265 928
Size: 971 1075 24 267 4147
Fragments arranged by size:
4147 1075 971 267 24
BbvCI CC'TCA_GC
Cuts at: 3557 3600 3557
Size: 43 6441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4675 4709 1903
Size: 34 2738 34 3678
Fragments arranged by size:
3678 2738 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4376 5586 4376
Size: 1210 5274
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3742 5095 136
Size: 107 122 71 631 605 2070 1353 1525
Fragments arranged by size:
2070 1525 1353 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2115 6479 2115
Size: 4364 2120
BlpI GC'TnA_GC
Cuts at: 2060 2763 3901 2060
Size: 703 1138 4643
Fragments arranged by size:
4643 1138 703
Bme1580I G_kGCm'C
Cuts at: 1790 2371 2394 2484 3102 4545 5701 1790
Size: 581 23 90 618 1443 1156 2573
Fragments arranged by size:
2573 1443 1156 618 581 90 23
BmgBI CAC'GTC
Cuts at: 1318 2493 1318
Size: 1175 5309
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2137 4215 5173
Size: 416 53 83 399 24 720 2078 958
Cuts at: 5173 442
Size: 1753
Fragments arranged by size:
2078 1753 958 720 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3745 3745
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3659 5144 1239
Size: 2420 1485 2579
Fragments arranged by size:
2579 2420 1485
Bpu10I CC'TnA_GC
Cuts at: 2136 3557 3600 3709 3738 2136
Size: 1421 43 109 29 4882
Fragments arranged by size:
4882 1421 109 43 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 3085 4609 5474 5772 2144
Size: 941 1524 865 298 2856
Fragments arranged by size:
2856 1524 941 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3170 3304 3635 3644 5147 882
Size: 617 1671 134 331 9 1503 2219
Fragments arranged by size:
2219 1671 1503 617 331 134 9
BsaAI yAC'GTr
Cuts at: 493 1204 4275 493
Size: 711 3071 2702
Fragments arranged by size:
3071 2702 711
BsaBI GATnn'nnATC
Cuts at: 2212 6478 2212
Size: 4266 2218
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 2320 2603 2745
Size: 53 83 186 936 561 226 283 142
Cuts at: 2745 2804 3258 4291 4673 5308 275
Size: 59 454 1033 382 635 1451
Fragments arranged by size:
1451 1033 936 635 561 454 382 283
226 186 142 83 59 53
BsaWI w'CCGG_w
Cuts at: 2398 3213 4917 5315 5589 5736 6147 2398
Size: 815 1704 398 274 147 411 2735
Fragments arranged by size:
2735 1704 815 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2900 2930 3053 3083 1178
Size: 30 165 30 1497 30 123 30 4579
Fragments arranged by size:
4579 1497 165 123 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2294 2792 2795 3737 1186
Size: 1108 498 3 942 3933
Fragments arranged by size:
3933 1108 942 498 3
BseYI C'CCAG_C
Cuts at: 2362 2463 2988 3057 3631 5687 6183 2362
Size: 101 525 69 574 2056 496 2663
Fragments arranged by size:
2663 2056 574 525 496 101 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2111 2633 2638 2906 1163
Size: 573 375 522 5 268 4741
Fragments arranged by size:
4741 573 522 375 268 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3190 3934 4695 5723 1540
Size: 275 186 1189 744 761 1028 2301
Fragments arranged by size:
2301 1189 1028 761 744 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 2137 2625 2736 3071 3123 3159 3243 4545
Size: 76 488 111 335 52 36 84 1302
Cuts at: 4545 4630 5701 2061
Size: 85 1071 2844
Fragments arranged by size:
2844 1302 1071 488 335 111 85 84
76 52 36
BsiWI C'GTAC_G
Cuts at: 2070 2070
Size: 6484
BsmI GAATG_Cn'
Cuts at: 3995 4088 3995
Size: 93 6391
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2137 2371 2394 2484 2625 2736
Size: 399 271 76 234 23 90 141 111
Cuts at: 2736 3071 3102 3123 3159 3243 3803 4545 4630
Size: 335 31 21 36 84 560 742 85
Cuts at: 4630 5701 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 560 399 335 271 234
141 111 90 85 84 76 36 31
23 21
BspEI T'CCGG_A
Cuts at: 2398 6147 2398
Size: 3749 2735
BspHI T'CATG_A
Cuts at: 2097 4373 6103 2097
Size: 2276 1730 2478
Fragments arranged by size:
2478 2276 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2298 2928 3670 3757 3839 844
Size: 1454 630 742 87 82 3489
Fragments arranged by size:
3489 1454 742 630 87 82
BsrBI CCG'CTC
Cuts at: 1394 1823 3742 4371 1394
Size: 429 1919 629 3507
Fragments arranged by size:
3507 1919 629 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2461 4979 5153 65
Size: 1183 1213 2518 174 1396
Fragments arranged by size:
2518 1396 1213 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2831 3185 3189 5128 5315 1700
Size: 110 1021 354 4 1939 187 2869
Fragments arranged by size:
2869 1939 1021 354 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6484
BssHII G'CGCG_C
Cuts at: 1925 2701 2788 1925
Size: 776 87 5621
Fragments arranged by size:
5621 776 87
BssSI C'ACGA_G
Cuts at: 2538 4544 5556 2538
Size: 2006 1012 3466
Fragments arranged by size:
3466 2006 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6484
BstBI TT'CG_AA
Cuts at: 5288 5288
Size: 6484
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6484
BstXI CCAn_nnnn'nTGG
Cuts at: 2191 2191
Size: 6484
BstYI r'GATC_y
Cuts at: 1972 2115 3814 4181 4566 4583 6035 6121 6133
Size: 143 1699 367 385 17 1452 86 12
Cuts at: 6133 6479 1972
Size: 346 1977
Fragments arranged by size:
1977 1699 1452 385 367 346 143 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2580 3498 3666 513
Size: 552 939 576 918 168 3331
Fragments arranged by size:
3331 939 918 576 552 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2779 487
Size: 683 56 1553 4192
Fragments arranged by size:
4192 1553 683 56
BtsI GCAGTG_nn'
Cuts at: 2650 3576 4000 4794 4814 2650
Size: 926 424 794 20 4320
Fragments arranged by size:
4320 926 794 424 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 3788 3788
Size: 6484
DraIII CAC_nnn'GTG
Cuts at: 2375 3054 2375
Size: 679 5805
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2218 5491 809
Size: 507 902 3273 1802
Fragments arranged by size:
3273 1802 902 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2357 2880 3042
Size: 54 1425 50 275 18 527 523 162
Cuts at: 3042 3187 3279 3592 3610 3743 3931 4820 6449
Size: 145 92 313 18 133 188 889 1629
Cuts at: 6449 8
Size: 43
Fragments arranged by size:
1629 1425 889 527 523 313 275 188
162 145 133 92 54 50 43 18
18
EagI C'GGCC_G
Cuts at: 1537 1812 3187 3931 1537
Size: 275 1375 744 4090
Fragments arranged by size:
4090 1375 744 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3140 3347 3759 4414 1353
Size: 1787 207 412 655 3423
Fragments arranged by size:
3423 1787 655 412 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5071 5445 5591 387
Size: 1064 138 3482 374 146 1280
Fragments arranged by size:
3482 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3069 3069
Size: 6484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3659 4547 5144 5931 6199 1239
Size: 393 2027 888 597 787 268 1524
Fragments arranged by size:
2027 1524 888 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2638 2774 3719 3758 2638
Size: 136 945 39 5364
Fragments arranged by size:
5364 945 136 39
EcoO109I rG'GnC_Cy
Cuts at: 2167 3627 2167
Size: 1460 5024
EcoRI G'AATT_C
Cuts at: 3793 4171 3793
Size: 378 6106
FspI TGC'GCA
Cuts at: 4990 4990
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 2039 2323 2748 2770 2807 5631 1678
Size: 361 284 425 22 37 2824 2531
Fragments arranged by size:
2824 2531 425 361 284 37 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2237 2269 3053 3085 3351
Size: 32 135 32 665 32 784 32 266
Cuts at: 3351 3383 5125 5157 5199 5231 1373
Size: 32 1742 32 42 32 2626
Fragments arranged by size:
2626 1742 784 665 266 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3828 4074 669
Size: 988 281 1890 246 3079
Fragments arranged by size:
3079 1890 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KasI G'GCGC_C
Cuts at: 2319 2744 2803 2319
Size: 425 59 6000
Fragments arranged by size:
6000 425 59
KpnI G_GTAC'C
Cuts at: 2314 3809 2314
Size: 1495 4989
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5598 5782 1305
Size: 4293 184 2007
Fragments arranged by size:
4293 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2882 3044 3281 3612 10
Size: 54 1425 343 1050 162 237 331 2882
Fragments arranged by size:
2882 1425 1050 343 331 237 162 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2672 2936 3095 3236 3513 3515 4442
Size: 648 1506 264 159 141 277 2 927
Cuts at: 4442 4801 4960 518
Size: 359 159 2042
Fragments arranged by size:
2042 1506 927 648 359 277 264 159
159 141 2
MspA1I CmG'CkG
Cuts at: 1606 2362 2463 2828 3002 3432 3471 3604 3900
Size: 756 101 365 174 430 39 133 296
Cuts at: 3900 4577 5725 5970 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 756 677 430 365 296 245
174 133 101 39
NaeI GCC'GGC
Cuts at: 2833 3187 2833
Size: 354 6130
NarI GG'CG_CC
Cuts at: 2320 2745 2804 2320
Size: 425 59 6000
Fragments arranged by size:
6000 425 59
NcoI C'CATG_G
Cuts at: 513 1065 2004 3498 3666 513
Size: 552 939 1494 168 3331
Fragments arranged by size:
3331 1494 939 552 168
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NgoMIV G'CCGG_C
Cuts at: 2831 3185 2831
Size: 354 6130
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NspI r_CATG'y
Cuts at: 1132 1229 2677 3842 5387 1132
Size: 97 1448 1165 1545 2229
Fragments arranged by size:
2229 1545 1448 1165 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6484
PciI A'CATG_T
Cuts at: 5383 5383
Size: 6484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6484
PfoI T'CCnGG_A
Cuts at: 3071 3071
Size: 6484
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2167 3627 2167
Size: 1460 5024
PshAI GACnn'nnGTC
Cuts at: 1613 3524 1613
Size: 1911 4573
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PstI C_TGCA'G
Cuts at: 830 2175 2849 3836 830
Size: 1345 674 987 3478
Fragments arranged by size:
3478 1345 987 674
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 2463 3604 2463
Size: 1141 5343
SacI G_AGCT'C
Cuts at: 3071 3071
Size: 6484
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SanDI GG'GwC_CC
Cuts at: 3627 3627
Size: 6484
SapI GCTCTTCn'nnn_
Cuts at: 3347 3347
Size: 6484
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 4732 6444 4732
Size: 1712 4772
SexAI A'CCwGG_T
Cuts at: 2371 2371
Size: 6484
SfcI C'TryA_G
Cuts at: 826 1045 2171 2503 2845 3484 3758 3832 4967
Size: 219 1126 332 342 639 274 74 1135
Cuts at: 4967 5648 5839 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1126 681 639 342 332 274
219 191 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 3742 3742
Size: 6484
SfoI GGC'GCC
Cuts at: 2321 2746 2805 2321
Size: 425 59 6000
Fragments arranged by size:
6000 425 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 3811 1872
Size: 1939 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 3064 3647 4588 5489 5751
Size: 197 926 216 905 583 941 901 262
Cuts at: 5751 820
Size: 1553
Fragments arranged by size:
1553 941 926 905 901 583 262 216
197
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 3842 1132
Size: 2710 3774
SspI AAT'ATT
Cuts at: 5 52 4408 5
Size: 47 4356 2081
Fragments arranged by size:
4356 2081 47
StuI AGG'CCT
Cuts at: 1517 3714 3728 1517
Size: 2197 14 4273
Fragments arranged by size:
4273 2197 14
StyI C'CwwG_G
Cuts at: 513 1065 2004 3498 3666 3678 3923 513
Size: 552 939 1494 168 12 245 3074
Fragments arranged by size:
3074 1494 939 552 245 168 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2311 4511 4528 4681 4866 5311 325
Size: 1255 731 2200 17 153 185 445 1498
Fragments arranged by size:
2200 1498 1255 731 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4730 6442 96
Size: 274 80 33 51 1443 2753 1712 138
Fragments arranged by size:
2753 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6444 6476 6444
Size: 32 6452
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6484
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XhoI C'TCGA_G
Cuts at: 1943 3647 1943
Size: 1704 4780
XmaI C'CCGG_G
Cuts at: 1870 3809 1870
Size: 1939 4545
XmnI GAAnn'nnTTC
Cuts at: 4613 4613
Size: 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2095 4292 276
Size: 53 83 186 936 561 2197 2468
Fragments arranged by size:
2468 2197 936 561 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BsmBI BstZ17I
Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SrfI SwaI
XcmI