Restriction map for
pFN21AE3420
(Circular) (Six-base) MAPSORT of: pFN21AE3420.seq Check: 8274 from: 1 to: 6154
pFN21AE3420 6154 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3964 278
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3497 1937
Size: 1560 4594
Acc65I G'GTAC_C
Cuts at: 3475 3475
Size: 6154
AclI AA'CG_TT
Cuts at: 1563 2746 4281 4654 1563
Size: 1183 1535 373 3063
Fragments arranged by size:
3063 1535 1183 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2589 3429 4217 5601 5869 1632
Size: 957 840 788 1384 268 1917
Fragments arranged by size:
1917 1384 957 840 788 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5957
AflIII A'CryG_T
Cuts at: 1203 3221 5053 6030 1203
Size: 2018 1832 977 1327
Fragments arranged by size:
2018 1832 1327 977
AgeI A'CCGG_T
Cuts at: 4985 4985
Size: 6154
AhdI GACnn_n'nnGTC
Cuts at: 4883 4883
Size: 6154
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6154
AlwNI CAG_nnn'CTG
Cuts at: 3897 5469 3897
Size: 1572 4582
ApaLI G'TGCA_C
Cuts at: 4211 5367 4211
Size: 1156 4998
ApoI r'AATT_y
Cuts at: 1493 2140 2280 2287 2407 2475 2559 2658 2895
Size: 647 140 7 120 68 84 99 237
Cuts at: 2895 2979 3231 3315 3463 3689 3841 5950 1493
Size: 84 252 84 148 226 152 2109 1697
Fragments arranged by size:
2109 1697 647 252 237 226 152 148
140 120 99 84 84 84 68 7
AseI AT'TA_AT
Cuts at: 160 1032 4708 160
Size: 872 3676 1606
Fragments arranged by size:
3676 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
AvaI C'yCGr_G
Cuts at: 1870 1943 3479 1870
Size: 73 1536 4545
Fragments arranged by size:
4545 1536 73
BamHI G'GATC_C
Cuts at: 3484 3851 3484
Size: 367 5787
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3475 4930 618
Size: 325 217 627 1688 1455 1842
Fragments arranged by size:
1842 1688 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3473 1391
Size: 2082 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5183
BbvCI CC'TCA_GC
Cuts at: 3124 3124
Size: 6154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4345 4379 1903
Size: 34 2408 34 3678
Fragments arranged by size:
3678 2408 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4046 5256 4046
Size: 1210 4944
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6154
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4765 136
Size: 107 122 71 631 605 3093 1525
Fragments arranged by size:
3093 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6149 6149
Size: 6154
BlpI GC'TnA_GC
Cuts at: 3571 3571
Size: 6154
Bme1580I G_kGCm'C
Cuts at: 1790 4215 5371 1790
Size: 2425 1156 2573
Fragments arranged by size:
2573 2425 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2661 3885 4843
Size: 416 53 83 399 24 1244 1224 958
Cuts at: 4843 442
Size: 1753
Fragments arranged by size:
1753 1244 1224 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6154
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3266 3266
Size: 6154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2505 2841 3009 3093 3177 3261 3345 4814
Size: 1266 336 168 84 84 84 84 1469
Cuts at: 4814 1239
Size: 2579
Fragments arranged by size:
2579 1469 1266 336 168 84 84 84
84
Bpu10I CC'TnA_GC
Cuts at: 3124 3124
Size: 6154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2254 4279 5144 5442 2254
Size: 2025 865 298 2966
Fragments arranged by size:
2966 2025 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4817 882
Size: 617 3318 2219
Fragments arranged by size:
3318 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3945 493
Size: 711 2741 2702
Fragments arranged by size:
2741 2702 711
BsaBI GATnn'nnATC
Cuts at: 6148 6148
Size: 6154
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3961 4343 4978 275
Size: 53 83 186 936 2428 382 635 1451
Fragments arranged by size:
2428 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2735 4587 4985 5259 5406 5817 2735
Size: 1852 398 274 147 411 3072
Fragments arranged by size:
3072 1852 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5929
Fragments arranged by size:
5929 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6154
BseYI C'CCAG_C
Cuts at: 5357 5853 5357
Size: 496 5658
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5581
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3604 4365 5393 1540
Size: 275 186 1603 761 1028 2301
Fragments arranged by size:
2301 1603 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4215 4300 5371 4215
Size: 85 1071 4998
Fragments arranged by size:
4998 1071 85
BsmI GAATG_Cn'
Cuts at: 2768 3665 3758 2768
Size: 897 93 5164
Fragments arranged by size:
5164 897 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3473 4215 4300 5371 1391
Size: 399 1683 742 85 1071 2174
Fragments arranged by size:
2174 1683 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5817 5817
Size: 6154
BspHI T'CATG_A
Cuts at: 4043 5773 4043
Size: 1730 4424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3509 844
Size: 2665 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4041 1394
Size: 429 2218 3507
Fragments arranged by size:
3507 2218 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4649 4823 65
Size: 1183 3401 174 1396
Fragments arranged by size:
3401 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4798 4985 1700
Size: 110 2988 187 2869
Fragments arranged by size:
2988 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2832 96
Size: 2736 3418
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6154
BssSI C'ACGA_G
Cuts at: 4214 5226 4214
Size: 1012 5142
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6154
BstBI TT'CG_AA
Cuts at: 4958 4958
Size: 6154
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6154
BstYI r'GATC_y
Cuts at: 1972 3484 3851 4236 4253 5705 5791 5803 6149
Size: 1512 367 385 17 1452 86 12 346
Cuts at: 6149 1972
Size: 1977
Fragments arranged by size:
1977 1512 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5602
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5415
Fragments arranged by size:
5415 683 56
BtsI GCAGTG_nn'
Cuts at: 3670 4464 4484 3670
Size: 794 20 5340
Fragments arranged by size:
5340 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6119
DraI TTT'AAA
Cuts at: 2332 2947 3458 2332
Size: 615 511 5028
Fragments arranged by size:
5028 615 511
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5161 809
Size: 507 3845 1802
Fragments arranged by size:
3845 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2069 3601 4490
Size: 54 1425 50 275 18 239 1532 889
Cuts at: 4490 6119 8
Size: 1629 43
Fragments arranged by size:
1629 1532 1425 889 275 239 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3601 1537
Size: 275 1789 4090
Fragments arranged by size:
4090 1789 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 2428 2565 3405 4084 1353
Size: 650 425 137 840 679 3423
Fragments arranged by size:
3423 840 679 650 425 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4741 5115 5261 387
Size: 1064 138 3152 374 146 1280
Fragments arranged by size:
3152 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2505 2589 2841 3009 3093 3177 3261
Size: 393 873 84 252 168 84 84 84
Cuts at: 3261 3345 3429 4217 4814 5601 5869 1239
Size: 84 84 788 597 787 268 1524
Fragments arranged by size:
1524 873 788 787 597 393 268 252
168 84 84 84 84 84 84
EcoRI G'AATT_C
Cuts at: 2475 2559 2895 3315 3463 3841 2475
Size: 84 336 420 148 378 4788
Fragments arranged by size:
4788 420 378 336 148 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2109 2109
Size: 6154
FspI TGC'GCA
Cuts at: 4660 4660
Size: 6154
HaeII r_GCGC'y
Cuts at: 1678 5301 1678
Size: 3623 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4795 4827 4869 4901 1373
Size: 32 135 32 3223 32 42 32 2626
Fragments arranged by size:
3223 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3498 3744 669
Size: 988 281 1560 246 3079
Fragments arranged by size:
3079 1560 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2708 2772 2940 3024 3360 3444 748
Size: 1960 64 168 84 336 84 3458
Fragments arranged by size:
3458 1960 336 168 84 84 64
HpaI GTT'AAC
Cuts at: 3744 3744
Size: 6154
KpnI G_GTAC'C
Cuts at: 3479 3479
Size: 6154
MfeI C'AATT_G
Cuts at: 3753 3753
Size: 6154
MluI A'CGCG_T
Cuts at: 6030 6030
Size: 6154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5268 5452 1305
Size: 3963 184 2007
Fragments arranged by size:
3963 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2071 10
Size: 54 1425 343 239 4093
Fragments arranged by size:
4093 1425 343 239 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4112 4471 4630 518
Size: 648 2946 359 159 2042
Fragments arranged by size:
2946 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3570 4247 5395 5640 1606
Size: 1964 677 1148 245 2120
Fragments arranged by size:
2120 1964 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5602
NdeI CA'TA_TG
Cuts at: 387 2428 387
Size: 2041 4113
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6154
NotI GC'GGCC_GC
Cuts at: 3601 3601
Size: 6154
NspI r_CATG'y
Cuts at: 1132 1229 2365 3225 3512 5057 1132
Size: 97 1136 860 287 1545 2229
Fragments arranged by size:
2229 1545 1136 860 287 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6154
PciI A'CATG_T
Cuts at: 3221 5053 3221
Size: 1832 4322
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6154
PmeI GTTT'AAAC
Cuts at: 3458 3458
Size: 6154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4223 4255 5736 5768 4223
Size: 32 1481 32 4609
Fragments arranged by size:
4609 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6154
PsiI TTA'TAA
Cuts at: 3724 3724
Size: 6154
PstI C_TGCA'G
Cuts at: 830 3506 830
Size: 2676 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6154
SalI G'TCGA_C
Cuts at: 1936 3496 1936
Size: 1560 4594
SapI GCTCTTCn'nnn_
Cuts at: 2428 2428
Size: 6154
SbfI CC_TGCA'GG
Cuts at: 3506 3506
Size: 6154
ScaI AGT'ACT
Cuts at: 4402 6114 4402
Size: 1712 4442
SfcI C'TryA_G
Cuts at: 826 1045 3502 4637 5318 5509 826
Size: 219 2457 1135 681 191 1471
Fragments arranged by size:
2457 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6154
SmaI CCC'GGG
Cuts at: 1872 3481 1872
Size: 1609 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2233 4258 5159 5421 820
Size: 197 926 290 2025 901 262 1553
Fragments arranged by size:
2025 1553 926 901 290 262 197
SnaBI TAC'GTA
Cuts at: 493 3945 493
Size: 3452 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6154
SphI G_CATG'C
Cuts at: 1132 2365 3512 1132
Size: 1233 1147 3774
Fragments arranged by size:
3774 1233 1147
SspI AAT'ATT
Cuts at: 5 52 2244 4078 5
Size: 47 2192 1834 2081
Fragments arranged by size:
2192 2081 1834 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6154
StyI C'CwwG_G
Cuts at: 513 1065 3593 513
Size: 552 2528 3074
Fragments arranged by size:
3074 2528 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4181 4198 4351 4536 4981 325
Size: 1255 2601 17 153 185 445 1498
Fragments arranged by size:
2601 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2808 2832 3218
Size: 274 80 33 51 1443 831 24 386
Cuts at: 3218 3420 4400 6112 96
Size: 202 980 1712 138
Fragments arranged by size:
1712 1443 980 831 386 274 202 138
80 51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6114 6146 6114
Size: 32 6122
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6154
XbaI T'CTAG_A
Cuts at: 3490 3490
Size: 6154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6154
XmaI C'CCGG_G
Cuts at: 1870 3479 1870
Size: 1609 4545
XmnI GAAnn'nnTTC
Cuts at: 4283 4283
Size: 6154
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3962 276
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI