Restriction map for
pFN21AE3405
(Circular) (Six-base) MAPSORT of: pFN21AE3405.seq Check: 8536 from: 1 to: 5341
pFN21AE3405 5341 bp
With 182 enzymes: SgfI *
March 28, 2012 12:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2489 3151 278
Size: 53 83 186 936 953 662 2468
Fragments arranged by size:
2468 953 936 662 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2163 2684 1937
Size: 226 521 4594
Fragments arranged by size:
4594 521 226
Acc65I G'GTAC_C
Cuts at: 2662 2662
Size: 5341
AclI AA'CG_TT
Cuts at: 1563 3468 3841 1563
Size: 1905 373 3063
Fragments arranged by size:
3063 1905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3404 4788 5056 1632
Size: 1772 1384 268 1917
Fragments arranged by size:
1917 1772 1384 268
AfeI AGC'GCT
Cuts at: 2114 2114
Size: 5341
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5144
AflIII A'CryG_T
Cuts at: 1203 4240 5217 1203
Size: 3037 977 1327
Fragments arranged by size:
3037 1327 977
AgeI A'CCGG_T
Cuts at: 4172 4172
Size: 5341
AhdI GACnn_n'nnGTC
Cuts at: 4070 4070
Size: 5341
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5341
AlwNI CAG_nnn'CTG
Cuts at: 2386 2449 3084 4656 2386
Size: 63 635 1572 3071
Fragments arranged by size:
3071 1572 635 63
ApaI G_GGCC'C
Cuts at: 2021 2564 2021
Size: 543 4798
ApaLI G'TGCA_C
Cuts at: 3398 4554 3398
Size: 1156 4185
ApoI r'AATT_y
Cuts at: 1493 2650 2876 3028 5137 1493
Size: 1157 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3895 160
Size: 872 2863 1606
Fragments arranged by size:
2863 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 2500 2666 1870
Size: 73 83 474 166 4545
Fragments arranged by size:
4545 474 166 83 73
BamHI G'GATC_C
Cuts at: 2671 3038 2671
Size: 367 4974
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2126 2329 2662 4117 618
Size: 325 217 627 339 203 333 1455 1842
Fragments arranged by size:
1842 1455 627 339 333 325 217 203
BanII G_rGCy'C
Cuts at: 1391 2021 2318 2369 2544 2564 2585 2660 1391
Size: 630 297 51 175 20 21 75 4072
Fragments arranged by size:
4072 630 297 175 75 51 21 20
BbeI G_GCGC'C
Cuts at: 2130 2130
Size: 5341
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2183 928
Size: 971 284 4086
Fragments arranged by size:
4086 971 284
BbvCI CC'TCA_GC
Cuts at: 2408 2436 2408
Size: 28 5313
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2596 2630 3532 3566 1903
Size: 34 659 34 902 34 3678
Fragments arranged by size:
3678 902 659 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3233 4443 3233
Size: 1210 4131
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2282 3952 136
Size: 107 122 71 631 605 610 1670 1525
Fragments arranged by size:
1670 1525 631 610 605 122 107 71
BglII A'GATC_T
Cuts at: 2214 5336 2214
Size: 3122 2219
BlpI GC'TnA_GC
Cuts at: 2454 2758 2454
Size: 304 5037
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2564 3402 4558 1790
Size: 231 543 838 1156 2573
Fragments arranged by size:
2573 1156 838 543 231
BmgBI CAC'GTC
Cuts at: 1318 2100 1318
Size: 782 4559
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3072 4030 442
Size: 416 53 83 399 24 1655 958 1753
Fragments arranged by size:
1753 1655 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 4001 1239
Size: 965 1797 2579
Fragments arranged by size:
2579 1797 965
Bpu10I CC'TnA_GC
Cuts at: 2408 2436 2408
Size: 28 5313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3466 4331 4629 3466
Size: 865 298 4178
Fragments arranged by size:
4178 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2431 4004 882
Size: 617 932 1573 2219
Fragments arranged by size:
2219 1573 932 617
BsaAI yAC'GTr
Cuts at: 493 1204 3132 493
Size: 711 1928 2702
Fragments arranged by size:
2702 1928 711
BsaBI GATnn'nnATC
Cuts at: 5335 5335
Size: 5341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2127 2160 2486 3148
Size: 53 83 186 936 594 33 326 662
Cuts at: 3148 3530 4165 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 662 635 594 382 326 186
83 53 33
BsaWI w'CCGG_w
Cuts at: 3774 4172 4446 4593 5004 3774
Size: 398 274 147 411 4111
Fragments arranged by size:
4111 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5116
Fragments arranged by size:
5116 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2326 2528 1186
Size: 1140 202 3999
Fragments arranged by size:
3999 1140 202
BseYI C'CCAG_C
Cuts at: 2625 4544 5040 2625
Size: 1919 496 2926
Fragments arranged by size:
2926 1919 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2129 2399 1163
Size: 573 393 270 4105
Fragments arranged by size:
4105 573 393 270
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2229 2791 3552 4580 1540
Size: 275 186 228 562 761 1028 2301
Fragments arranged by size:
2301 1028 761 562 275 228 186
BsiHKAI G_wGCw'C
Cuts at: 2318 2460 2544 3402 3487 4558 2318
Size: 142 84 858 85 1071 3101
Fragments arranged by size:
3101 1071 858 142 85 84
BsmI GAATG_Cn'
Cuts at: 2852 2945 2852
Size: 93 5248
BsmBI CGTCTCn'nnnn_
Cuts at: 2477 2477
Size: 5341
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2318 2369 2460 2544 2564 2585
Size: 399 231 297 51 91 84 20 21
Cuts at: 2585 2660 3402 3487 4558 1391
Size: 75 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 297 231 91 85
84 75 51 21 20
BspEI T'CCGG_A
Cuts at: 5004 5004
Size: 5341
BspHI T'CATG_A
Cuts at: 3230 4960 3230
Size: 1730 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2696 844
Size: 1852 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2239 3228 1394
Size: 429 416 989 3507
Fragments arranged by size:
3507 989 429 416
BsrDI GCAATG_nn'
Cuts at: 65 1248 3836 4010 65
Size: 1183 2588 174 1396
Fragments arranged by size:
2588 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2123 2228 3985 4172 1700
Size: 110 313 105 1757 187 2869
Fragments arranged by size:
2869 1757 313 187 110 105
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5341
BssSI C'ACGA_G
Cuts at: 3401 4413 3401
Size: 1012 4329
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2100 794
Size: 1306 4035
BstBI TT'CG_AA
Cuts at: 4145 4145
Size: 5341
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5341
BstYI r'GATC_y
Cuts at: 1972 2187 2214 2671 3038 3423 3440 4892 4978
Size: 215 27 457 367 385 17 1452 86
Cuts at: 4978 4990 5336 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 457 385 367 346 215 86
27 17 12
Bsu36I CC'TnA_GG
Cuts at: 2525 2525
Size: 5341
BtgI C'CryG_G
Cuts at: 513 1065 2004 2535 513
Size: 552 939 531 3319
Fragments arranged by size:
3319 939 552 531
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4602
Fragments arranged by size:
4602 683 56
BtsI GCAGTG_nn'
Cuts at: 2278 2857 3651 3671 2278
Size: 579 794 20 3948
Fragments arranged by size:
3948 794 579 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5306
DraI TTT'AAA
Cuts at: 2645 2645
Size: 5341
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4348 809
Size: 507 3032 1802
Fragments arranged by size:
3032 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 2230 2532
Size: 54 1425 50 275 18 396 4 302
Cuts at: 2532 2788 3677 5306 8
Size: 256 889 1629 43
Fragments arranged by size:
1629 1425 889 396 302 275 256 54
50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2226 2788 1537
Size: 275 414 562 4090
Fragments arranged by size:
4090 562 414 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2573 3271 1353
Size: 1220 698 3423
Fragments arranged by size:
3423 1220 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3928 4302 4448 387
Size: 1064 138 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2316 2542 2316
Size: 226 5115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 3404 4001 4788 5056 1239
Size: 393 572 1200 597 787 268 1524
Fragments arranged by size:
1524 1200 787 597 572 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2524 2524
Size: 5341
EcoO109I rG'GnC_Cy
Cuts at: 2032 2560 2561 2032
Size: 528 1 4812
Fragments arranged by size:
4812 528 1
EcoRI G'AATT_C
Cuts at: 2650 3028 2650
Size: 378 4963
FseI GG_CCGG'CC
Cuts at: 2232 2232
Size: 5341
FspI TGC'GCA
Cuts at: 3847 3847
Size: 5341
HaeII r_GCGC'y
Cuts at: 1678 2116 2130 2143 4488 1678
Size: 438 14 13 2345 2531
Fragments arranged by size:
2531 2345 438 14 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3982 4014 4056 4088 1373
Size: 32 135 32 2410 32 42 32 2626
Fragments arranged by size:
2626 2410 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2164 2685 2931 669
Size: 988 281 226 521 246 3079
Fragments arranged by size:
3079 988 521 281 246 226
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5341
HpaI GTT'AAC
Cuts at: 2931 2931
Size: 5341
KasI G'GCGC_C
Cuts at: 2126 2126
Size: 5341
KpnI G_GTAC'C
Cuts at: 2666 2666
Size: 5341
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 5341
MluI A'CGCG_T
Cuts at: 5217 5217
Size: 5341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2394 4455 4639 1305
Size: 1089 2061 184 2007
Fragments arranged by size:
2061 2007 1089 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2534 10
Size: 54 1425 343 702 2817
Fragments arranged by size:
2817 1425 702 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3299 3658 3817 518
Size: 648 2133 359 159 2042
Fragments arranged by size:
2133 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2383 2599 2757 3434 4582 4827 1606
Size: 777 216 158 677 1148 245 2120
Fragments arranged by size:
2120 1148 777 677 245 216 158
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 5341
NarI GG'CG_CC
Cuts at: 2127 2127
Size: 5341
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3850
Fragments arranged by size:
3850 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5341
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5341
NotI GC'GGCC_GC
Cuts at: 2788 2788
Size: 5341
NspI r_CATG'y
Cuts at: 1132 1229 2699 4244 1132
Size: 97 1470 1545 2229
Fragments arranged by size:
2229 1545 1470 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5341
PciI A'CATG_T
Cuts at: 4240 4240
Size: 5341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5341
PmeI GTTT'AAAC
Cuts at: 2645 2645
Size: 5341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 4923 4955 3410
Size: 32 1481 32 3796
Fragments arranged by size:
3796 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2032 2032
Size: 5341
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5341
PsiI TTA'TAA
Cuts at: 2911 2911
Size: 5341
PspOMI G'GGCC_C
Cuts at: 2017 2560 2017
Size: 543 4798
PspXI vC'TCGA_Gb
Cuts at: 2500 2500
Size: 5341
PstI C_TGCA'G
Cuts at: 830 2214 2693 830
Size: 1384 479 3478
Fragments arranged by size:
3478 1384 479
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5341
PvuII CAG'CTG
Cuts at: 2383 2599 2383
Size: 216 5125
SacI G_AGCT'C
Cuts at: 2318 2544 2318
Size: 226 5115
SalI G'TCGA_C
Cuts at: 1936 2162 2683 1936
Size: 226 521 4594
Fragments arranged by size:
4594 521 226
SanDI GG'GwC_CC
Cuts at: 2032 2032
Size: 5341
SapI GCTCTTCn'nnn_
Cuts at: 2573 2573
Size: 5341
SbfI CC_TGCA'GG
Cuts at: 2693 2693
Size: 5341
ScaI AGT'ACT
Cuts at: 3589 5301 3589
Size: 1712 3629
SfcI C'TryA_G
Cuts at: 826 1045 2210 2689 3824 4505 4696 826
Size: 219 1165 479 1135 681 191 1471
Fragments arranged by size:
1471 1165 1135 681 479 219 191
SfoI GGC'GCC
Cuts at: 2128 2128
Size: 5341
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5341
SmaI CCC'GGG
Cuts at: 1872 2028 2668 1872
Size: 156 640 4545
Fragments arranged by size:
4545 640 156
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2500 3445 4346 4608 820
Size: 197 926 557 945 901 262 1553
Fragments arranged by size:
1553 945 926 901 557 262 197
SnaBI TAC'GTA
Cuts at: 493 3132 493
Size: 2639 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5341
SphI G_CATG'C
Cuts at: 1132 2699 1132
Size: 1567 3774
SspI AAT'ATT
Cuts at: 5 52 3265 5
Size: 47 3213 2081
Fragments arranged by size:
3213 2081 47
StuI AGG'CCT
Cuts at: 1517 2519 1517
Size: 1002 4339
StyI C'CwwG_G
Cuts at: 513 1065 2004 2430 2493 2780 513
Size: 552 939 426 63 287 3074
Fragments arranged by size:
3074 939 552 426 287 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2270 3368 3385 3538 3723 4168 325
Size: 1255 690 1098 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1098 690 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3587 5299 96
Size: 274 80 33 51 1443 1610 1712 138
Fragments arranged by size:
1712 1610 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5301 5333 5301
Size: 32 5309
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5341
XbaI T'CTAG_A
Cuts at: 2677 2677
Size: 5341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 2179
Size: 5341
XhoI C'TCGA_G
Cuts at: 1943 2500 1943
Size: 557 4784
XmaI C'CCGG_G
Cuts at: 1870 2026 2666 1870
Size: 156 640 4545
Fragments arranged by size:
4545 640 156
XmnI GAAnn'nnTTC
Cuts at: 3470 3470
Size: 5341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2487 3149 276
Size: 53 83 186 936 953 662 2468
Fragments arranged by size:
2468 953 936 662 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I DraIII EcoRV FalI FspAI NruI NsiI
PacI PfoI PmlI PsrI RsrII SacII SexAI SfiI
SrfI SwaI