Restriction map for
pFN21AE3396
(Circular) (Six-base) MAPSORT of: pFN21AE3396.seq Check: 1031 from: 1 to: 8530
pFN21AE3396 8530 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6340 278
Size: 53 83 186 936 4804 2468
Fragments arranged by size:
4804 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2965 4656 5873 1937
Size: 1028 1691 1217 4594
Fragments arranged by size:
4594 1691 1217 1028
Acc65I G'GTAC_C
Cuts at: 3963 5851 3963
Size: 1888 6642
AclI AA'CG_TT
Cuts at: 1563 6657 7030 1563
Size: 5094 373 3063
Fragments arranged by size:
5094 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2429 2537 2739 3682 4550 6593 7977 8245
Size: 797 108 202 943 868 2043 1384 268
Cuts at: 8245 1632
Size: 1917
Fragments arranged by size:
2043 1917 1384 943 868 797 268 202
108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8333
AflIII A'CryG_T
Cuts at: 1203 4131 7429 8406 1203
Size: 2928 3298 977 1327
Fragments arranged by size:
3298 2928 1327 977
AgeI A'CCGG_T
Cuts at: 7361 7361
Size: 8530
AhdI GACnn_n'nnGTC
Cuts at: 2611 7259 2611
Size: 4648 3882
AleI CACnn'nnGTG
Cuts at: 1166 2985 3119 1166
Size: 1819 134 6577
Fragments arranged by size:
6577 1819 134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5750 1184
Size: 4566 3964
AlwNI CAG_nnn'CTG
Cuts at: 3217 4444 6273 7845 3217
Size: 1227 1829 1572 3902
Fragments arranged by size:
3902 1829 1572 1227
ApaLI G'TGCA_C
Cuts at: 6587 7743 6587
Size: 1156 7374
ApoI r'AATT_y
Cuts at: 1493 2303 2449 3314 4709 5607 5839 6065 6217
Size: 810 146 865 1395 898 232 226 152
Cuts at: 6217 8326 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1395 898 865 810 232 226
152 146
AseI AT'TA_AT
Cuts at: 160 1032 2766 3683 7084 160
Size: 872 1734 917 3401 1606
Fragments arranged by size:
3401 1734 1606 917 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8530
AvaI C'yCGr_G
Cuts at: 1870 1943 3450 5855 1870
Size: 73 1507 2405 4545
Fragments arranged by size:
4545 2405 1507 73
AvrII C'CTAG_G
Cuts at: 2375 2375
Size: 8530
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3993 4026 3993
Size: 33 8497
BamHI G'GATC_C
Cuts at: 5860 6227 5860
Size: 367 8163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3837 3963 5851 7306 618
Size: 325 217 627 2050 126 1888 1455 1842
Fragments arranged by size:
2050 1888 1842 1455 627 325 217 126
BanII G_rGCy'C
Cuts at: 1391 3636 5743 5849 1391
Size: 2245 2107 106 4072
Fragments arranged by size:
4072 2245 2107 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3173 5445 928
Size: 971 1274 2272 4013
Fragments arranged by size:
4013 2272 1274 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6721 6755 1903
Size: 34 4784 34 3678
Fragments arranged by size:
4784 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4764 6422 7632 4764
Size: 1658 1210 5662
Fragments arranged by size:
5662 1658 1210
BclI T'GATC_A
Cuts at: 1548 5682 1548
Size: 4134 4396
BfrBI ATG'CAT
Cuts at: 4245 4245
Size: 8530
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7141 136
Size: 107 122 71 631 605 5469 1525
Fragments arranged by size:
5469 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2694 2887 3256 8525 2694
Size: 193 369 5269 2699
Fragments arranged by size:
5269 2699 369 193
BlpI GC'TnA_GC
Cuts at: 3630 5947 3630
Size: 2317 6213
Bme1580I G_kGCm'C
Cuts at: 1790 2982 5829 6591 7747 1790
Size: 1192 2847 762 1156 2573
Fragments arranged by size:
2847 2573 1192 1156 762
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8530
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2149 3583 4975
Size: 416 53 83 399 24 732 1434 1392
Cuts at: 4975 5006 6261 7219 442
Size: 31 1255 958 1753
Fragments arranged by size:
1753 1434 1392 1255 958 732 416 399
83 53 31 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2584 2880 3620 4350 5765 7190 1239
Size: 1345 296 740 730 1415 1425 2579
Fragments arranged by size:
2579 1425 1415 1345 740 730 296
Bpu10I CC'TnA_GC
Cuts at: 3918 5413 3918
Size: 1495 7035
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2253 3074 4578 5699 6655 7520 7818 2253
Size: 821 1504 1121 956 865 298 2965
Fragments arranged by size:
2965 1504 1121 956 865 821 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7193 882
Size: 617 5694 2219
Fragments arranged by size:
5694 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3601 6321 493
Size: 711 2397 2720 2702
Fragments arranged by size:
2720 2702 2397 711
BsaBI GATnn'nnATC
Cuts at: 4337 8524 4337
Size: 4187 4343
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6337 6719 7354 275
Size: 53 83 186 936 4804 382 635 1451
Fragments arranged by size:
4804 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5372 6963 7361 7635 7782 8193 5372
Size: 1591 398 274 147 411 5709
Fragments arranged by size:
5709 1591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4763 4793 1178
Size: 30 165 30 3360 30 4915
Fragments arranged by size:
4915 3360 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2505 3165 3509 1186
Size: 856 463 660 344 6207
Fragments arranged by size:
6207 856 660 463 344
BseYI C'CCAG_C
Cuts at: 3798 7733 8229 3798
Size: 3935 496 4099
Fragments arranged by size:
4099 3935 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2221 4457 1163
Size: 573 485 2236 5236
Fragments arranged by size:
5236 2236 573 485
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5980 6741 7769 1540
Size: 275 186 3979 761 1028 2301
Fragments arranged by size:
3979 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3413 3636 4992 6591 6676 7747 3413
Size: 223 1356 1599 85 1071 4196
Fragments arranged by size:
4196 1599 1356 1071 223 85
BsmI GAATG_Cn'
Cuts at: 2352 2833 5139 5725 6041 6134 2352
Size: 481 2306 586 316 93 4748
Fragments arranged by size:
4748 2306 586 481 316 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2153 2153
Size: 8530
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2982 3413 3636 4992 5743 5829 5849
Size: 399 1192 431 223 1356 751 86 20
Cuts at: 5849 6591 6676 7747 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1356 1192 1071 751 742 431 399
223 86 85 20
BspEI T'CCGG_A
Cuts at: 8193 8193
Size: 8530
BspHI T'CATG_A
Cuts at: 2188 6419 8149 2188
Size: 4231 1730 2569
Fragments arranged by size:
4231 2569 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5262 5451 5885 844
Size: 4418 189 434 3489
Fragments arranged by size:
4418 3489 434 189
BsrBI CCG'CTC
Cuts at: 1394 1823 5749 6417 1394
Size: 429 3926 668 3507
Fragments arranged by size:
3926 3507 668 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4396 7025 7199 65
Size: 1183 3148 2629 174 1396
Fragments arranged by size:
3148 2629 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7174 7361 1700
Size: 110 5364 187 2869
Fragments arranged by size:
5364 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4335 96
Size: 4239 4291
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8530
BssSI C'ACGA_G
Cuts at: 4889 6590 7602 4889
Size: 1701 1012 5817
Fragments arranged by size:
5817 1701 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2424 4444 5356 794
Size: 1630 2020 912 3968
Fragments arranged by size:
3968 2020 1630 912
BstBI TT'CG_AA
Cuts at: 5204 7334 5204
Size: 2130 6400
BstEII G'GTnAC_C
Cuts at: 1184 3494 1184
Size: 2310 6220
BstXI CCAn_nnnn'nTGG
Cuts at: 4411 4904 5408 5822 4411
Size: 493 504 414 7119
Fragments arranged by size:
7119 504 493 414
BstYI r'GATC_y
Cuts at: 1972 2546 2694 2707 2794 2887 3256 4414 5509
Size: 574 148 13 87 93 369 1158 1095
Cuts at: 5509 5860 6227 6612 6629 8081 8167 8179 8525
Size: 351 367 385 17 1452 86 12 346
Cuts at: 8525 1972
Size: 1977
Fragments arranged by size:
1977 1452 1158 1095 574 385 369 367
351 346 148 93 87 86 17 13
12
Bsu36I CC'TnA_GG
Cuts at: 3042 5427 3042
Size: 2385 6145
BtgI C'CryG_G
Cuts at: 513 1065 2004 4782 513
Size: 552 939 2778 4261
Fragments arranged by size:
4261 2778 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5060 487
Size: 683 56 3834 3957
Fragments arranged by size:
3957 3834 683 56
BtsI GCAGTG_nn'
Cuts at: 3114 3126 3981 4146 5321 5492 6046 6840 6860
Size: 12 855 165 1175 171 554 794 20
Cuts at: 6860 3114
Size: 4784
Fragments arranged by size:
4784 1175 855 794 554 171 165 20
12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8530
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4771 4806 5061 5096 529
Size: 35 4207 35 255 35 3963
Fragments arranged by size:
4207 3963 255 35 35 35
DraI TTT'AAA
Cuts at: 5834 5834
Size: 8530
DraIII CAC_nnn'GTG
Cuts at: 4435 5277 4435
Size: 842 7688
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3204 5238 7537 809
Size: 507 1888 2034 2299 1802
Fragments arranged by size:
2299 2034 1888 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3507 5977 6866
Size: 54 1425 50 275 18 1677 2470 889
Cuts at: 6866 8495 8
Size: 1629 43
Fragments arranged by size:
2470 1677 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5977 1537
Size: 275 4165 4090
Fragments arranged by size:
4165 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2032 2451 3428 3849 4602 6460 1353
Size: 679 419 977 421 753 1858 3423
Fragments arranged by size:
3423 1858 977 753 679 421 419
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7117 7491 7637 387
Size: 1064 138 5528 374 146 1280
Fragments arranged by size:
5528 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3634 3634
Size: 8530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2429 2537 2584 2739 2880 3620 3682
Size: 393 797 108 47 155 141 740 62
Cuts at: 3682 4350 4550 5765 6593 7190 7977 8245 1239
Size: 668 200 1215 828 597 787 268 1524
Fragments arranged by size:
1524 1215 828 797 787 740 668 597
393 268 200 155 141 108 62 47
EcoO109I rG'GnC_Cy
Cuts at: 2396 3214 3444 3950 5422 5638 2396
Size: 818 230 506 1472 216 5288
Fragments arranged by size:
5288 1472 818 506 230 216
EcoRI G'AATT_C
Cuts at: 5839 6217 5839
Size: 378 8152
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2404 2829 4631 2404
Size: 425 1802 6303
Fragments arranged by size:
6303 1802 425
FspI TGC'GCA
Cuts at: 7036 7036
Size: 8530
HaeII r_GCGC'y
Cuts at: 1678 7677 1678
Size: 5999 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2597 2629 2858 2890 3160
Size: 32 135 32 1025 32 229 32 270
Cuts at: 3160 3192 3206 3225 3238 3257 4220 4252 4763
Size: 32 14 19 13 19 963 32 511
Cuts at: 4763 4795 5302 5334 5516 5548 5573 5605 7171
Size: 32 507 32 182 32 25 32 1566
Cuts at: 7171 7203 7245 7277 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1566 1025 963 511 507 270 229
182 135 42 32 32 32 32 32
32 32 32 32 32 32 32 25
19 19 14 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2993 4137 4792 5874 6120 669
Size: 988 281 1055 1144 655 1082 246 3079
Fragments arranged by size:
3079 1144 1082 1055 988 655 281 246
HindIII A'AGCT_T
Cuts at: 748 2748 2850 5654 748
Size: 2000 102 2804 3624
Fragments arranged by size:
3624 2804 2000 102
HpaI GTT'AAC
Cuts at: 6120 6120
Size: 8530
KpnI G_GTAC'C
Cuts at: 3967 5855 3967
Size: 1888 6642
MfeI C'AATT_G
Cuts at: 3062 3539 3663 6129 3062
Size: 477 124 2466 5463
Fragments arranged by size:
5463 2466 477 124
MluI A'CGCG_T
Cuts at: 8406 8406
Size: 8530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3050 7644 7828 1305
Size: 1745 4594 184 2007
Fragments arranged by size:
4594 2007 1745 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3509 10
Size: 54 1425 343 1677 5031
Fragments arranged by size:
5031 1677 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2985 3119 4151 5536 6488 6847 7006
Size: 648 1819 134 1032 1385 952 359 159
Cuts at: 7006 518
Size: 2042
Fragments arranged by size:
2042 1819 1385 1032 952 648 359 159
134
MspA1I CmG'CkG
Cuts at: 1606 3629 4528 5560 5946 6623 7771 8016 1606
Size: 2023 899 1032 386 677 1148 245 2120
Fragments arranged by size:
2120 2023 1148 1032 899 677 386 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7039
Fragments arranged by size:
7039 939 552
NdeI CA'TA_TG
Cuts at: 387 5023 387
Size: 4636 3894
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8530
NotI GC'GGCC_GC
Cuts at: 5977 5977
Size: 8530
NruI TCG'CGA
Cuts at: 5045 5045
Size: 8530
NsiI A_TGCA'T
Cuts at: 4247 4247
Size: 8530
NspI r_CATG'y
Cuts at: 1132 1229 4135 4245 5359 5888 7433 1132
Size: 97 2906 110 1114 529 1545 2229
Fragments arranged by size:
2906 2229 1545 1114 529 110 97
PasI CC'CwG_GG
Cuts at: 1348 3925 5759 1348
Size: 2577 1834 4119
Fragments arranged by size:
4119 2577 1834
PciI A'CATG_T
Cuts at: 4131 7429 4131
Size: 3298 5232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3505 3737 3960 4837 1108
Size: 2397 232 223 877 4801
Fragments arranged by size:
4801 2397 877 232 223
PfoI T'CCnGG_A
Cuts at: 3636 3636
Size: 8530
PmeI GTTT'AAAC
Cuts at: 5834 5834
Size: 8530
PmlI CAC'GTG
Cuts at: 3601 3601
Size: 8530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6599 6631 8112 8144 6599
Size: 32 1481 32 6985
Fragments arranged by size:
6985 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3214 3950 5422 5638 3214
Size: 736 1472 216 6106
Fragments arranged by size:
6106 1472 736 216
PshAI GACnn'nnGTC
Cuts at: 1613 2054 1613
Size: 441 8089
PsiI TTA'TAA
Cuts at: 4290 6100 4290
Size: 1810 6720
PstI C_TGCA'G
Cuts at: 830 2832 3121 5437 5882 830
Size: 2002 289 2316 445 3478
Fragments arranged by size:
3478 2316 2002 445 289
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8530
PvuII CAG'CTG
Cuts at: 3629 4528 5560 3629
Size: 899 1032 6599
Fragments arranged by size:
6599 1032 899
SacI G_AGCT'C
Cuts at: 3636 3636
Size: 8530
SalI G'TCGA_C
Cuts at: 1936 5872 1936
Size: 3936 4594
SapI GCTCTTCn'nnn_
Cuts at: 3428 3849 3428
Size: 421 8109
SbfI CC_TGCA'GG
Cuts at: 5882 5882
Size: 8530
ScaI AGT'ACT
Cuts at: 6778 8490 6778
Size: 1712 6818
SfcI C'TryA_G
Cuts at: 826 1045 2828 3117 4554 4653 5433 5878 7013
Size: 219 1783 289 1437 99 780 445 1135
Cuts at: 7013 7694 7885 826
Size: 681 191 1471
Fragments arranged by size:
1783 1471 1437 1135 780 681 445 289
219 191 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8530
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8530
SmaI CCC'GGG
Cuts at: 1872 5857 1872
Size: 3985 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2268 3053 4593 5714 6634 7535
Size: 197 926 325 785 1540 1121 920 901
Cuts at: 7535 7797 820
Size: 262 1553
Fragments arranged by size:
1553 1540 1121 926 920 901 785 325
262 197
SnaBI TAC'GTA
Cuts at: 493 6321 493
Size: 5828 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8530
SphI G_CATG'C
Cuts at: 1132 5359 5888 1132
Size: 4227 529 3774
Fragments arranged by size:
4227 3774 529
SspI AAT'ATT
Cuts at: 5 52 2176 3330 6454 5
Size: 47 2124 1154 3124 2081
Fragments arranged by size:
3124 2124 2081 1154 47
StuI AGG'CCT
Cuts at: 1517 2125 1517
Size: 608 7922
StyI C'CwwG_G
Cuts at: 513 1065 2004 2375 2937 3588 3736 3810 4476
Size: 552 939 371 562 651 148 74 666
Cuts at: 4476 4809 4818 5631 5796 5969 513
Size: 333 9 813 165 173 3074
Fragments arranged by size:
3074 939 813 666 651 562 552 371
333 173 165 148 74 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3779 4448 4518 6557 6574 6727 6912
Size: 1255 2199 669 70 2039 17 153 185
Cuts at: 6912 7357 325
Size: 445 1498
Fragments arranged by size:
2199 2039 1498 1255 669 445 185 153
70 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2105 4128 4335
Size: 274 80 33 51 1443 128 2023 207
Cuts at: 4335 6776 8488 96
Size: 2441 1712 138
Fragments arranged by size:
2441 2023 1712 1443 274 207 138 128
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8490 8522 8490
Size: 32 8498
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8530
XbaI T'CTAG_A
Cuts at: 5866 5866
Size: 8530
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5278 5278
Size: 8530
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8530
XmaI C'CCGG_G
Cuts at: 1870 5855 1870
Size: 3985 4545
XmnI GAAnn'nnTTC
Cuts at: 4611 6659 4611
Size: 2048 6482
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6338 276
Size: 53 83 186 936 4804 2468
Fragments arranged by size:
4804 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NruI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BplI
BsiWI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV PacI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI