Restriction map for
pFN21AE3393
(Circular) (Six-base) MAPSORT of: pFN21AE3393.seq Check: 2710 from: 1 to: 8269
pFN21AE3393 8269 bp
With 182 enzymes: SgfI *
September 21, 2017 12:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4990 4990
Size: 8269
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6079 278
Size: 53 83 186 936 4543 2468
Fragments arranged by size:
4543 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2270 2700 5612 1937
Size: 333 430 2912 4594
Fragments arranged by size:
4594 2912 430 333
Acc65I G'GTAC_C
Cuts at: 5171 5590 5171
Size: 419 7850
AclI AA'CG_TT
Cuts at: 1563 6396 6769 1563
Size: 4833 373 3063
Fragments arranged by size:
4833 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2478 2732 2877 2952 3028 3708 4298 4827
Size: 846 254 145 75 76 680 590 529
Cuts at: 4827 5124 5433 6332 7716 7984 1632
Size: 297 309 899 1384 268 1917
Fragments arranged by size:
1917 1384 899 846 680 590 529 309
297 268 254 145 76 75
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8072
AflIII A'CryG_T
Cuts at: 1203 7168 8145 1203
Size: 5965 977 1327
Fragments arranged by size:
5965 1327 977
AgeI A'CCGG_T
Cuts at: 7100 7100
Size: 8269
AhdI GACnn_n'nnGTC
Cuts at: 6998 6998
Size: 8269
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8269
AlwNI CAG_nnn'CTG
Cuts at: 5510 6012 7584 5510
Size: 502 1572 6195
Fragments arranged by size:
6195 1572 502
ApaLI G'TGCA_C
Cuts at: 6326 7482 6326
Size: 1156 7113
ApoI r'AATT_y
Cuts at: 1493 2663 3105 3136 3436 3774 4129 4180 4699
Size: 1170 442 31 300 338 355 51 519
Cuts at: 4699 5578 5804 5956 8065 1493
Size: 879 226 152 2109 1697
Fragments arranged by size:
2109 1697 1170 879 519 442 355 338
300 226 152 51 31
AseI AT'TA_AT
Cuts at: 160 1032 5249 6823 160
Size: 872 4217 1574 1606
Fragments arranged by size:
4217 1606 1574 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8269
AvaI C'yCGr_G
Cuts at: 1870 1943 3426 5594 1870
Size: 73 1483 2168 4545
Fragments arranged by size:
4545 2168 1483 73
BamHI G'GATC_C
Cuts at: 5599 5966 5599
Size: 367 7902
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2908 5171 5590 7045
Size: 325 217 627 282 839 2263 419 1455
Cuts at: 7045 618
Size: 1842
Fragments arranged by size:
2263 1842 1455 839 627 419 325 282
217
BanII G_rGCy'C
Cuts at: 1391 2730 5588 1391
Size: 1339 2858 4072
Fragments arranged by size:
4072 2858 1339
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2426 2505 2982 3480 3714 3770 4393
Size: 971 527 79 477 498 234 56 623
Cuts at: 4393 4488 928
Size: 95 4709
Fragments arranged by size:
4709 971 623 527 498 477 234 95
79 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2036 2070 6460 6494 1903
Size: 34 99 34 4390 34 3678
Fragments arranged by size:
4390 3678 99 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2586 6161 7371 2586
Size: 3575 1210 3484
Fragments arranged by size:
3575 3484 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8269
BfrBI ATG'CAT
Cuts at: 4767 4767
Size: 8269
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 6880 136
Size: 107 122 71 631 605 337 4871 1525
Fragments arranged by size:
4871 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2438 3022 4405 8264 2438
Size: 584 1383 3859 2443
Fragments arranged by size:
3859 2443 1383 584
BlpI GC'TnA_GC
Cuts at: 5686 5686
Size: 8269
Bme1580I G_kGCm'C
Cuts at: 1790 6330 7486 1790
Size: 4540 1156 2573
Fragments arranged by size:
4540 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8269
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3569 6000 6958
Size: 416 53 83 399 24 2152 2431 958
Cuts at: 6958 442
Size: 1753
Fragments arranged by size:
2431 2152 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8269
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2460 3441 4978 5371 2460
Size: 981 1537 393 5358
Fragments arranged by size:
5358 1537 981 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2388 2936 6929 1239
Size: 1149 548 3993 2579
Fragments arranged by size:
3993 2579 1149 548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2840 4635 5097 6394 7259 7557 2840
Size: 1795 462 1297 865 298 3552
Fragments arranged by size:
3552 1795 1297 865 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5126 6932 882
Size: 617 3627 1806 2219
Fragments arranged by size:
3627 2219 1806 617
BsaAI yAC'GTr
Cuts at: 493 1204 6060 493
Size: 711 4856 2702
Fragments arranged by size:
4856 2702 711
BsaBI GATnn'nnATC
Cuts at: 3027 8263 3027
Size: 5236 3033
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6076 6458 7093 275
Size: 53 83 186 936 4543 382 635 1451
Fragments arranged by size:
4543 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2716 6702 7100 7374 7521 7932 2716
Size: 3986 398 274 147 411 3053
Fragments arranged by size:
3986 3053 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2184 2214 1178
Size: 30 165 30 781 30 7233
Fragments arranged by size:
7233 781 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3209 3995 5449 1186
Size: 2023 786 1454 4006
Fragments arranged by size:
4006 2023 1454 786
BseYI C'CCAG_C
Cuts at: 2597 2851 7472 7968 2597
Size: 254 4621 496 2898
Fragments arranged by size:
4621 2898 496 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4209 1163
Size: 573 2473 5223
Fragments arranged by size:
5223 2473 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5719 6480 7508 1540
Size: 275 186 3718 761 1028 2301
Fragments arranged by size:
3718 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2730 6330 6415 7486 2730
Size: 3600 85 1071 3513
Fragments arranged by size:
3600 3513 1071 85
BsmI GAATG_Cn'
Cuts at: 3123 4740 5567 5780 5873 3123
Size: 1617 827 213 93 5519
Fragments arranged by size:
5519 1617 827 213 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2302 2516 2302
Size: 214 8055
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2730 5588 6330 6415 7486 1391
Size: 399 940 2858 742 85 1071 2174
Fragments arranged by size:
2858 2174 1071 940 742 399 85
BspEI T'CCGG_A
Cuts at: 7932 7932
Size: 8269
BspHI T'CATG_A
Cuts at: 6158 7888 6158
Size: 1730 6539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4990 5624 844
Size: 4146 634 3489
Fragments arranged by size:
4146 3489 634
BsrBI CCG'CTC
Cuts at: 1394 1823 6156 1394
Size: 429 4333 3507
Fragments arranged by size:
4333 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4854 6764 6938 65
Size: 1183 3606 1910 174 1396
Fragments arranged by size:
3606 1910 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6913 7100 1700
Size: 110 5103 187 2869
Fragments arranged by size:
5103 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5188 96
Size: 5092 3177
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8269
BssSI C'ACGA_G
Cuts at: 6329 7341 6329
Size: 1012 7257
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8269
BstBI TT'CG_AA
Cuts at: 7073 7073
Size: 8269
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8269
BstXI CCAn_nnnn'nTGG
Cuts at: 2152 2152
Size: 8269
BstYI r'GATC_y
Cuts at: 1972 2222 2438 3022 4405 4676 5599 5966 6351
Size: 250 216 584 1383 271 923 367 385
Cuts at: 6351 6368 7820 7906 7918 8264 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1383 923 584 385 367 346
271 250 216 86 17 12
BstZ17I GTA'TAC
Cuts at: 2271 2271
Size: 8269
Bsu36I CC'TnA_GG
Cuts at: 2913 3345 2913
Size: 432 7837
BtgI C'CryG_G
Cuts at: 513 1065 2274 513
Size: 552 1209 6508
Fragments arranged by size:
6508 1209 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4578 487
Size: 683 56 3352 4178
Fragments arranged by size:
4178 3352 683 56
BtsI GCAGTG_nn'
Cuts at: 2488 5785 6579 6599 2488
Size: 3297 794 20 4158
Fragments arranged by size:
4158 3297 794 20
ClaI AT'CG_AT
Cuts at: 1554 3357 1554
Size: 1803 6466
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2991 3026 529
Size: 35 2427 35 5772
Fragments arranged by size:
5772 2427 35 35
DraI TTT'AAA
Cuts at: 4017 5573 4017
Size: 1556 6713
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7276 809
Size: 507 5960 1802
Fragments arranged by size:
5960 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4441 5716 6605
Size: 54 1425 50 275 18 2611 1275 889
Cuts at: 6605 8234 8
Size: 1629 43
Fragments arranged by size:
2611 1629 1425 1275 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5716 1537
Size: 275 3904 4090
Fragments arranged by size:
4090 3904 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2451 2481 2865 2898 2928 3038 3047 3285
Size: 1098 30 384 33 30 110 9 238
Cuts at: 3285 3329 3378 3456 3630 3636 5216 6199 1353
Size: 44 49 78 174 6 1580 983 3423
Fragments arranged by size:
3423 1580 1098 983 384 238 174 110
78 49 44 33 30 30 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6856 7230 7376 387
Size: 1064 138 5267 374 146 1280
Fragments arranged by size:
5267 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2728 2728
Size: 8269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 2478 2732 2877 2936 2952 3028
Size: 393 756 90 254 145 59 16 76
Cuts at: 3028 3708 4298 4827 5124 5433 6332 6929 7716
Size: 680 590 529 297 309 899 597 787
Cuts at: 7716 7984 1239
Size: 268 1524
Fragments arranged by size:
1524 899 787 756 680 597 590 529
393 309 297 268 254 145 90 76
59 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2132 2132
Size: 8269
EcoO109I rG'GnC_Cy
Cuts at: 2177 2177
Size: 8269
EcoRI G'AATT_C
Cuts at: 3136 5578 5956 3136
Size: 2442 378 5449
Fragments arranged by size:
5449 2442 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2457 2457
Size: 8269
FspI TGC'GCA
Cuts at: 6775 6775
Size: 8269
HaeII r_GCGC'y
Cuts at: 1678 2509 7416 1678
Size: 831 4907 2531
Fragments arranged by size:
4907 2531 831
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3611 3643 3837 3869 4390
Size: 32 135 32 2039 32 194 32 521
Cuts at: 4390 4422 5120 5152 6910 6942 6984 7016 1373
Size: 32 698 32 1758 32 42 32 2626
Fragments arranged by size:
2626 2039 1758 698 521 194 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2253 5613 5859 669
Size: 988 281 315 3360 246 3079
Fragments arranged by size:
3360 3079 988 315 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8269
HpaI GTT'AAC
Cuts at: 5859 5859
Size: 8269
KpnI G_GTAC'C
Cuts at: 5175 5594 5175
Size: 419 7850
MfeI C'AATT_G
Cuts at: 2150 5868 2150
Size: 3718 4551
MluI A'CGCG_T
Cuts at: 8145 8145
Size: 8269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2170 4270 4795 5026 7383 7567 1305
Size: 865 2100 525 231 2357 184 2007
Fragments arranged by size:
2357 2100 2007 865 525 231 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4443 10
Size: 54 1425 343 2611 3836
Fragments arranged by size:
3836 2611 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2618 3063 3240 3840 5513 6227 6586
Size: 648 1452 445 177 600 1673 714 359
Cuts at: 6586 6745 518
Size: 159 2042
Fragments arranged by size:
2042 1673 1452 714 648 600 445 359
177 159
MspA1I CmG'CkG
Cuts at: 1606 2601 2855 3143 4805 5685 6362 7510 7755
Size: 995 254 288 1662 880 677 1148 245
Cuts at: 7755 1606
Size: 2120
Fragments arranged by size:
2120 1662 1148 995 880 677 288 254
245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7717
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8269
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8269
NotI GC'GGCC_GC
Cuts at: 5716 5716
Size: 8269
NsiI A_TGCA'T
Cuts at: 4769 4769
Size: 8269
NspI r_CATG'y
Cuts at: 1132 1229 4042 5627 7172 1132
Size: 97 2813 1585 1545 2229
Fragments arranged by size:
2813 2229 1585 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8269
PciI A'CATG_T
Cuts at: 7168 7168
Size: 8269
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5234 1108
Size: 4126 4143
PmeI GTTT'AAAC
Cuts at: 5573 5573
Size: 8269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6338 6370 7851 7883 6338
Size: 32 1481 32 6724
Fragments arranged by size:
6724 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2177 2177
Size: 8269
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8269
PsiI TTA'TAA
Cuts at: 2964 4424 5839 2964
Size: 1460 1415 5394
Fragments arranged by size:
5394 1460 1415
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4293 4325 4293
Size: 32 8237
PstI C_TGCA'G
Cuts at: 830 3535 5296 5621 830
Size: 2705 1761 325 3478
Fragments arranged by size:
3478 2705 1761 325
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8269
PvuII CAG'CTG
Cuts at: 2601 2855 3143 4805 2601
Size: 254 288 1662 6065
Fragments arranged by size:
6065 1662 288 254
SacI G_AGCT'C
Cuts at: 2730 2730
Size: 8269
SalI G'TCGA_C
Cuts at: 1936 5611 1936
Size: 3675 4594
SbfI CC_TGCA'GG
Cuts at: 5621 5621
Size: 8269
ScaI AGT'ACT
Cuts at: 3200 6517 8229 3200
Size: 3317 1712 3240
Fragments arranged by size:
3317 3240 1712
SfcI C'TryA_G
Cuts at: 826 1045 2673 3531 4527 4995 5176 5292 5375
Size: 219 1628 858 996 468 181 116 83
Cuts at: 5375 5556 5617 6752 7433 7624 826
Size: 181 61 1135 681 191 1471
Fragments arranged by size:
1628 1471 1135 996 858 681 468 219
191 181 181 116 83 61
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8269
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8269
SmaI CCC'GGG
Cuts at: 1872 5596 1872
Size: 3724 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2819 3426 4650 5112 6373 7274
Size: 197 926 876 607 1224 462 1261 901
Cuts at: 7274 7536 820
Size: 262 1553
Fragments arranged by size:
1553 1261 1224 926 901 876 607 462
262 197
SnaBI TAC'GTA
Cuts at: 493 6060 493
Size: 5567 2702
SpeI A'CTAG_T
Cuts at: 152 4781 152
Size: 4629 3640
SphI G_CATG'C
Cuts at: 1132 5627 1132
Size: 4495 3774
SspI AAT'ATT
Cuts at: 5 52 3079 3217 4822 5393 6193 5
Size: 47 3027 138 1605 571 800 2081
Fragments arranged by size:
3027 2081 1605 800 571 138 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8269
StyI C'CwwG_G
Cuts at: 513 1065 3118 4872 5708 513
Size: 552 2053 1754 836 3074
Fragments arranged by size:
3074 2053 1754 836 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3859 6296 6313 6466 6651 7096 325
Size: 1255 2279 2437 17 153 185 445 1498
Fragments arranged by size:
2437 2279 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3198 4309 5188
Size: 274 80 33 51 1443 1221 1111 879
Cuts at: 5188 5342 5548 6515 8227 96
Size: 154 206 967 1712 138
Fragments arranged by size:
1712 1443 1221 1111 967 879 274 206
154 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3723 3755 8229 8261 3723
Size: 32 4474 32 3731
Fragments arranged by size:
4474 3731 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8269
XbaI T'CTAG_A
Cuts at: 5605 5605
Size: 8269
XhoI C'TCGA_G
Cuts at: 1943 3426 1943
Size: 1483 6786
XmaI C'CCGG_G
Cuts at: 1870 5594 1870
Size: 3724 4545
XmnI GAAnn'nnTTC
Cuts at: 6398 6398
Size: 8269
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6077 276
Size: 53 83 186 936 4543 2468
Fragments arranged by size:
4543 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI