Restriction map for
pFN21AE3388
(Circular) (Six-base) MAPSORT of: pFN21AE3388.seq Check: 478 from: 1 to: 8347
pFN21AE3388 8347 bp
With 182 enzymes: SgfI *
March 28, 2012 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4738 4738
Size: 8347
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6157 278
Size: 53 83 186 936 4621 2468
Fragments arranged by size:
4621 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5690 1937
Size: 3753 4594
Acc65I G'GTAC_C
Cuts at: 4568 5668 4568
Size: 1100 7247
AclI AA'CG_TT
Cuts at: 1563 6474 6847 1563
Size: 4911 373 3063
Fragments arranged by size:
4911 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3391 3747 3872 4493 4871 5122 6410 7794
Size: 1759 356 125 621 378 251 1288 1384
Cuts at: 7794 8062 1632
Size: 268 1917
Fragments arranged by size:
1917 1759 1384 1288 621 378 356 268
251 125
AfeI AGC'GCT
Cuts at: 2615 2615
Size: 8347
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8150
AflIII A'CryG_T
Cuts at: 1203 7246 8223 1203
Size: 6043 977 1327
Fragments arranged by size:
6043 1327 977
AgeI A'CCGG_T
Cuts at: 7178 7178
Size: 8347
AhdI GACnn_n'nnGTC
Cuts at: 3670 7076 3670
Size: 3406 4941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2798 3344 3805 1184
Size: 1614 546 461 5726
Fragments arranged by size:
5726 1614 546 461
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5412 5444 5412
Size: 32 8315
AlwNI CAG_nnn'CTG
Cuts at: 3753 4819 5029 5481 6090 7662 3753
Size: 1066 210 452 609 1572 4438
Fragments arranged by size:
4438 1572 1066 609 452 210
ApaLI G'TGCA_C
Cuts at: 2749 6404 7560 2749
Size: 3655 1156 3536
Fragments arranged by size:
3655 3536 1156
ApoI r'AATT_y
Cuts at: 1493 2597 5351 5656 5882 6034 8143 1493
Size: 1104 2754 305 226 152 2109 1697
Fragments arranged by size:
2754 2109 1697 1104 305 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4233 6901 160
Size: 872 3201 2668 1606
Fragments arranged by size:
3201 2668 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8347
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2090 3243 3819 4662 5672 1870
Size: 73 123 24 1153 576 843 1010 4545
Fragments arranged by size:
4545 1153 1010 843 576 123 73 24
AvrII C'CTAG_G
Cuts at: 4090 4090
Size: 8347
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2494 2527 2494
Size: 33 8314
BamHI G'GATC_C
Cuts at: 5197 5595 5677 6044 5197
Size: 398 82 367 7500
Fragments arranged by size:
7500 398 367 82
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 4568 5668 7123 618
Size: 325 217 627 234 2547 1100 1455 1842
Fragments arranged by size:
2547 1842 1455 1100 627 325 234 217
BanII G_rGCy'C
Cuts at: 1391 2091 2103 2460 3263 3590 5103 5146 5298
Size: 700 12 357 803 327 1513 43 152
Cuts at: 5298 5425 5525 5532 5666 1391
Size: 127 100 7 134 4072
Fragments arranged by size:
4072 1513 803 700 357 327 152 134
127 100 43 12 7
BbeI G_GCGC'C
Cuts at: 2025 2025
Size: 8347
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2165 2968 3049 3408 3453 3581 4499
Size: 971 266 803 81 359 45 128 918
Cuts at: 4499 4869 928
Size: 370 4406
Fragments arranged by size:
4406 971 918 803 370 359 266 128
81 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6538 6572 1903
Size: 34 4601 34 3678
Fragments arranged by size:
4601 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4997 6239 7449 4997
Size: 1242 1210 5895
Fragments arranged by size:
5895 1242 1210
BclI T'GATC_A
Cuts at: 1548 4068 1548
Size: 2520 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2264 2868
Size: 107 122 71 631 605 337 255 604
Cuts at: 2868 3722 4134 5642 6958 136
Size: 854 412 1508 1316 1525
Fragments arranged by size:
1525 1508 1316 854 631 605 604 412
337 255 122 107 71
BglII A'GATC_T
Cuts at: 3294 4713 8342 3294
Size: 1419 3629 3299
Fragments arranged by size:
3629 3299 1419
BlpI GC'TnA_GC
Cuts at: 3262 5618 5764 3262
Size: 2356 146 5845
Fragments arranged by size:
5845 2356 146
Bme1580I G_kGCm'C
Cuts at: 1790 2753 2864 3986 4813 6408 7564 1790
Size: 963 111 1122 827 1595 1156 2573
Fragments arranged by size:
2573 1595 1156 1122 963 827 111
BmgBI CAC'GTC
Cuts at: 1318 2139 2568 1318
Size: 821 429 7097
Fragments arranged by size:
7097 821 429
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2458 3260 4497
Size: 416 53 83 399 24 1041 802 1237
Cuts at: 4497 4690 6078 7036 442
Size: 193 1388 958 1753
Fragments arranged by size:
1753 1388 1237 1041 958 802 416 399
193 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8347
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 3845 2122
Size: 1723 6624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4475 4493 4640 5486 5561 7007 1239
Size: 3236 18 147 846 75 1446 2579
Fragments arranged by size:
3236 2579 1446 846 147 75 18
Bpu10I CC'TnA_GC
Cuts at: 5252 5252
Size: 8347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3638 5540 6472 7337 7635 3638
Size: 1902 932 865 298 4350
Fragments arranged by size:
4350 1902 932 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3762 7010 882
Size: 617 2263 3248 2219
Fragments arranged by size:
3248 2263 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6138 493
Size: 711 4934 2702
Fragments arranged by size:
4934 2702 711
BsaBI GATnn'nnATC
Cuts at: 4718 8341 4718
Size: 3623 4724
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 6154 6536 7171
Size: 53 83 186 936 489 4132 382 635
Cuts at: 7171 275
Size: 1451
Fragments arranged by size:
4132 1451 936 635 489 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3120 3764 6780 7178 7452 7599 8010 3120
Size: 644 3016 398 274 147 411 3457
Fragments arranged by size:
3457 3016 644 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4907 4937 5412 5442 1178
Size: 30 165 30 3504 30 475 30 4083
Fragments arranged by size:
4083 3504 475 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 3373 3376 3499 3608 3734 3974 4042
Size: 898 1289 3 123 109 126 240 68
Cuts at: 4042 4943 4946 5462 1186
Size: 901 3 516 4071
Fragments arranged by size:
4071 1289 901 898 516 240 126 123
109 68 3 3
BseYI C'CCAG_C
Cuts at: 3156 3205 3255 3282 4116 4535 5218 7550 8046
Size: 49 50 27 834 419 683 2332 496
Cuts at: 8046 3156
Size: 3457
Fragments arranged by size:
3457 2332 834 683 496 419 50 49
27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2456 4396 4418 4766 4892 5519 1163
Size: 573 720 1940 22 348 126 627 3991
Fragments arranged by size:
3991 1940 720 627 573 348 126 22
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2667 3582 3803 5797 6558 7586
Size: 275 186 666 915 221 1994 761 1028
Cuts at: 7586 1540
Size: 2301
Fragments arranged by size:
2301 1994 1028 915 761 666 275 221
186
BsiHKAI G_wGCw'C
Cuts at: 2451 2753 3525 3590 3623 4365 4847 5146 5230
Size: 302 772 65 33 742 482 299 84
Cuts at: 5230 5472 5532 6408 6493 7564 2451
Size: 242 60 876 85 1071 3234
Fragments arranged by size:
3234 1071 876 772 742 482 302 299
242 85 84 65 60 33
BsmI GAATG_Cn'
Cuts at: 3913 5858 5951 3913
Size: 1945 93 6309
Fragments arranged by size:
6309 1945 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3524 4663 5150 3524
Size: 1139 487 6721
Fragments arranged by size:
6721 1139 487
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2103 2451 2460 2753 2864 3263
Size: 399 301 12 348 9 293 111 399
Cuts at: 3263 3525 3590 3623 3986 4365 4813 4847 5103
Size: 262 65 33 363 379 448 34 256
Cuts at: 5103 5146 5230 5298 5425 5472 5525 5532 5666
Size: 43 84 68 127 47 53 7 134
Cuts at: 5666 6408 6493 7564 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 448 399 399 379 363
348 301 293 262 256 134 127 111
85 84 68 65 53 47 43 34
33 12 9 7
BspEI T'CCGG_A
Cuts at: 3120 8010 3120
Size: 4890 3457
BspHI T'CATG_A
Cuts at: 3066 4293 6236 7966 3066
Size: 1227 1943 1730 3447
Fragments arranged by size:
3447 1943 1730 1227
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4738 5702 844
Size: 3894 964 3489
Fragments arranged by size:
3894 3489 964
BsrBI CCG'CTC
Cuts at: 1394 1823 3220 5545 6234 1394
Size: 429 1397 2325 689 3507
Fragments arranged by size:
3507 2325 1397 689 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2542 2709 3711 6842 7016 65
Size: 1183 1294 167 1002 3131 174 1396
Fragments arranged by size:
3131 1396 1294 1183 1002 174 167
BsrFI r'CCGG_y
Cuts at: 1700 1810 6991 7178 1700
Size: 110 5181 187 2869
Fragments arranged by size:
5181 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8347
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8347
BssSI C'ACGA_G
Cuts at: 5360 6407 7419 5360
Size: 1047 1012 6288
Fragments arranged by size:
6288 1047 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2541 2757 3753 794
Size: 1747 216 996 5388
Fragments arranged by size:
5388 1747 996 216
BstBI TT'CG_AA
Cuts at: 7151 7151
Size: 8347
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8347
BstXI CCAn_nnnn'nTGG
Cuts at: 3331 4945 5062 3331
Size: 1614 117 6616
Fragments arranged by size:
6616 1614 117
BstYI r'GATC_y
Cuts at: 1972 2148 2293 3294 4158 4713 5197 5323 5595
Size: 176 145 1001 864 555 484 126 272
Cuts at: 5595 5677 6044 6429 6446 7898 7984 7996 8342
Size: 82 367 385 17 1452 86 12 346
Cuts at: 8342 1972
Size: 1977
Fragments arranged by size:
1977 1452 1001 864 555 484 385 367
346 272 176 145 126 86 82 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2820 2898 5061 5273 513
Size: 552 939 816 78 2163 212 3587
Fragments arranged by size:
3587 2163 939 816 552 212 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3285 4371 487
Size: 683 56 2059 1086 4463
Fragments arranged by size:
4463 2059 1086 683 56
BtsI GCAGTG_nn'
Cuts at: 2782 5158 5350 5863 6657 6677 2782
Size: 2376 192 513 794 20 4452
Fragments arranged by size:
4452 2376 794 513 192 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2752 2787 529
Size: 35 2188 35 6089
Fragments arranged by size:
6089 2188 35 35
DraI TTT'AAA
Cuts at: 5374 5651 5374
Size: 277 8070
DraIII CAC_nnn'GTG
Cuts at: 2757 2835 3499 3979 5274 2757
Size: 78 664 480 1295 5830
Fragments arranged by size:
5830 1295 664 480 78
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7354 809
Size: 507 6038 1802
Fragments arranged by size:
6038 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3513 3609 3800
Size: 54 1425 50 275 18 1683 96 191
Cuts at: 3800 4653 5794 6683 8312 8
Size: 853 1141 889 1629 43
Fragments arranged by size:
1683 1629 1425 1141 889 853 275 191
96 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3800 5794 1537
Size: 275 1988 1994 4090
Fragments arranged by size:
4090 1994 1988 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3443 5301 5585 6277 1353
Size: 2090 1858 284 692 3423
Fragments arranged by size:
3423 2090 1858 692 284
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2019 2043 2111 4101 5190 5345
Size: 1064 138 430 24 68 1990 1089 155
Cuts at: 5345 6934 7308 7454 387
Size: 1589 374 146 1280
Fragments arranged by size:
1990 1589 1280 1089 1064 430 374 155
146 138 68 24
EcoICRI GAG'CTC
Cuts at: 3588 5144 5530 3588
Size: 1556 386 6405
Fragments arranged by size:
6405 1556 386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3391 3747 3872 4475 4493 4640 4871
Size: 393 1759 356 125 603 18 147 231
Cuts at: 4871 5122 5486 5561 6410 7007 7794 8062 1239
Size: 251 364 75 849 597 787 268 1524
Fragments arranged by size:
1759 1524 849 787 603 597 393 364
356 268 251 231 147 125 75 18
EcoO109I rG'GnC_Cy
Cuts at: 2036 3160 4015 4972 5551 5560 2036
Size: 1124 855 957 579 9 4823
Fragments arranged by size:
4823 1124 957 855 579 9
EcoRI G'AATT_C
Cuts at: 5656 6034 5656
Size: 378 7969
EcoRV GAT'ATC
Cuts at: 4332 4332
Size: 8347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2395 5515 2395
Size: 3120 5227
FspI TGC'GCA
Cuts at: 5150 6853 5150
Size: 1703 6644
HaeII r_GCGC'y
Cuts at: 1678 2025 2617 2745 2803 5631 7494 1678
Size: 347 592 128 58 2828 1863 2531
Fragments arranged by size:
2828 2531 1863 592 347 128 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2468 2500 2540
Size: 32 135 32 488 32 376 32 40
Cuts at: 2540 2572 3479 3511 3656 3688 4907 4939 5308
Size: 32 907 32 145 32 1219 32 369
Cuts at: 5308 5340 6988 7020 7062 7094 1373
Size: 32 1648 32 42 32 2626
Fragments arranged by size:
2626 1648 1219 907 488 376 369 145
135 42 40 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2338 5691 5937 669
Size: 988 281 400 3353 246 3079
Fragments arranged by size:
3353 3079 988 400 281 246
HindIII A'AGCT_T
Cuts at: 748 2231 748
Size: 1483 6864
HpaI GTT'AAC
Cuts at: 5937 5937
Size: 8347
KasI G'GCGC_C
Cuts at: 2021 2021
Size: 8347
KpnI G_GTAC'C
Cuts at: 4572 5672 4572
Size: 1100 7247
MfeI C'AATT_G
Cuts at: 2497 5946 2497
Size: 3449 4898
MluI A'CGCG_T
Cuts at: 8223 8223
Size: 8347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2103 2176 2700 3288 7461 7645 1305
Size: 798 73 524 588 4173 184 2007
Fragments arranged by size:
4173 2007 798 588 524 184 73
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3611 10
Size: 54 1425 343 1779 4746
Fragments arranged by size:
4746 1779 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3326 4649 4943 6305 6664 6823 518
Size: 648 2160 1323 294 1362 359 159 2042
Fragments arranged by size:
2160 2042 1362 1323 648 359 294 159
MspA1I CmG'CkG
Cuts at: 1606 2943 3205 5194 5218 5763 6440 7588 7833
Size: 1337 262 1989 24 545 677 1148 245
Cuts at: 7833 1606
Size: 2120
Fragments arranged by size:
2120 1989 1337 1148 677 545 262 245
24
NarI GG'CG_CC
Cuts at: 2022 2022
Size: 8347
NcoI C'CATG_G
Cuts at: 513 1065 2004 2820 5061 513
Size: 552 939 816 2241 3799
Fragments arranged by size:
3799 2241 939 816 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8347
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8347
NotI GC'GGCC_GC
Cuts at: 5794 5794
Size: 8347
NspI r_CATG'y
Cuts at: 1132 1229 4648 5705 7250 1132
Size: 97 3419 1057 1545 2229
Fragments arranged by size:
3419 2229 1545 1057 97
PacI TTA_AT'TAA
Cuts at: 4233 4233
Size: 8347
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8347
PciI A'CATG_T
Cuts at: 7246 7246
Size: 8347
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2719 3298 4560 1108
Size: 1611 579 1262 4895
Fragments arranged by size:
4895 1611 1262 579
PfoI T'CCnGG_A
Cuts at: 5118 5118
Size: 8347
PmeI GTTT'AAAC
Cuts at: 5651 5651
Size: 8347
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5412 5444 6416 6448 7929 7961 5412
Size: 32 972 32 1481 32 5798
Fragments arranged by size:
5798 1481 972 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2036 3160 4015 4972 5551 5560 2036
Size: 1124 855 957 579 9 4823
Fragments arranged by size:
4823 1124 957 855 579 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8347
PsiI TTA'TAA
Cuts at: 5917 5917
Size: 8347
PstI C_TGCA'G
Cuts at: 830 2793 5111 5193 5699 830
Size: 1963 2318 82 506 3478
Fragments arranged by size:
3478 2318 1963 506 82
PvuI CG_AT'CG
Cuts at: 2001 2667 2001
Size: 666 7681
PvuII CAG'CTG
Cuts at: 2943 3205 5194 5218 2943
Size: 262 1989 24 6072
Fragments arranged by size:
6072 1989 262 24
SacI G_AGCT'C
Cuts at: 3590 5146 5532 3590
Size: 1556 386 6405
Fragments arranged by size:
6405 1556 386
SalI G'TCGA_C
Cuts at: 1936 5689 1936
Size: 3753 4594
SanDI GG'GwC_CC
Cuts at: 2036 4015 2036
Size: 1979 6368
SbfI CC_TGCA'GG
Cuts at: 2793 5699 2793
Size: 2906 5441
ScaI AGT'ACT
Cuts at: 3249 6595 8307 3249
Size: 3346 1712 3289
Fragments arranged by size:
3346 3289 1712
SfcI C'TryA_G
Cuts at: 826 1045 2789 4676 5107 5189 5695 6830 7511
Size: 219 1744 1887 431 82 506 1135 681
Cuts at: 7511 7702 826
Size: 191 1471
Fragments arranged by size:
1887 1744 1471 1135 681 506 431 219
191 82
SfoI GGC'GCC
Cuts at: 2023 2023
Size: 8347
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8347
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8347
SmaI CCC'GGG
Cuts at: 1872 5674 1872
Size: 3802 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3653 3819 4662 5555 6451 7352
Size: 197 926 1710 166 843 893 896 901
Cuts at: 7352 7614 820
Size: 262 1553
Fragments arranged by size:
1710 1553 926 901 896 893 843 262
197 166
SnaBI TAC'GTA
Cuts at: 493 6138 493
Size: 5645 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8347
SphI G_CATG'C
Cuts at: 1132 4648 5705 1132
Size: 3516 1057 3774
Fragments arranged by size:
3774 3516 1057
SspI AAT'ATT
Cuts at: 5 52 6271 5
Size: 47 6219 2081
Fragments arranged by size:
6219 2081 47
StuI AGG'CCT
Cuts at: 1517 3380 3566 3680 4763 1517
Size: 1863 186 114 1083 5101
Fragments arranged by size:
5101 1863 1083 186 114
StyI C'CwwG_G
Cuts at: 513 1065 2004 2820 3660 4020 4090 4572 4723
Size: 552 939 816 840 360 70 482 151
Cuts at: 4723 5061 5574 5786 513
Size: 338 513 212 3074
Fragments arranged by size:
3074 939 840 816 552 513 482 360
338 212 151 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2477 2911 3296 3596 4380 4955 5259
Size: 1255 897 434 385 300 784 575 304
Cuts at: 5259 6374 6391 6544 6729 7174 325
Size: 1115 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1115 897 784 575 445 434
385 304 300 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2466 3247 6593
Size: 274 80 33 51 1443 489 781 3346
Cuts at: 6593 8305 96
Size: 1712 138
Fragments arranged by size:
3346 1712 1443 781 489 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2564 2596 5587 5619 8307 8339 2564
Size: 32 2991 32 2688 32 2572
Fragments arranged by size:
2991 2688 2572 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8347
XbaI T'CTAG_A
Cuts at: 5683 5683
Size: 8347
XhoI C'TCGA_G
Cuts at: 1943 3819 4662 1943
Size: 1876 843 5628
Fragments arranged by size:
5628 1876 843
XmaI C'CCGG_G
Cuts at: 1870 5672 1870
Size: 3802 4545
XmnI GAAnn'nnTTC
Cuts at: 3542 5135 6476 3542
Size: 1593 1341 5413
Fragments arranged by size:
5413 1593 1341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6155 276
Size: 53 83 186 936 4621 2468
Fragments arranged by size:
4621 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
ApaI AscI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoNI
FseI FspAI NaeI NgoMIV NruI NsiI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI
SwaI XcmI