Restriction map for
pFN21AE3385
(Circular) (Six-base) MAPSORT of: pFN21AE3385.seq Check: 9139 from: 1 to: 8218
pFN21AE3385 8218 bp
With 182 enzymes: SgfI *
March 28, 2012 12:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6028 278
Size: 53 83 186 936 4492 2468
Fragments arranged by size:
4492 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2510 5466 5561 1937
Size: 573 2956 95 4594
Fragments arranged by size:
4594 2956 573 95
Acc65I G'GTAC_C
Cuts at: 5539 5539
Size: 8218
AclI AA'CG_TT
Cuts at: 1563 6345 6718 1563
Size: 4782 373 3063
Fragments arranged by size:
4782 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2714 2888 3368 3992 4947 6281 7665 7933
Size: 1082 174 480 624 955 1334 1384 268
Cuts at: 7933 1632
Size: 1917
Fragments arranged by size:
1917 1384 1334 1082 955 624 480 268
174
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8021
AflIII A'CryG_T
Cuts at: 1203 2035 2820 3153 3471 3622 7117 8094 1203
Size: 832 785 333 318 151 3495 977 1327
Fragments arranged by size:
3495 1327 977 832 785 333 318 151
AgeI A'CCGG_T
Cuts at: 4238 7049 4238
Size: 2811 5407
AhdI GACnn_n'nnGTC
Cuts at: 3157 6947 3157
Size: 3790 4428
AleI CACnn'nnGTG
Cuts at: 1166 3716 4115 1166
Size: 2550 399 5269
Fragments arranged by size:
5269 2550 399
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2231 3848 4242 4761 4776 5202 5316 5340
Size: 1047 1617 394 519 15 426 114 24
Cuts at: 5340 1184
Size: 4062
Fragments arranged by size:
4062 1617 1047 519 426 394 114 24
15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2690 2722 2690
Size: 32 8186
AlwNI CAG_nnn'CTG
Cuts at: 4066 4090 4165 5195 5961 7533 4066
Size: 24 75 1030 766 1572 4751
Fragments arranged by size:
4751 1572 1030 766 75 24
ApaI G_GGCC'C
Cuts at: 3112 5012 5372 3112
Size: 1900 360 5958
Fragments arranged by size:
5958 1900 360
ApaLI G'TGCA_C
Cuts at: 4457 5171 6275 7431 4457
Size: 714 1104 1156 5244
Fragments arranged by size:
5244 1156 1104 714
ApoI r'AATT_y
Cuts at: 1493 5527 5753 5905 8014 1493
Size: 4034 226 152 2109 1697
Fragments arranged by size:
4034 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6772 160
Size: 872 5740 1606
Fragments arranged by size:
5740 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8218
AvaI C'yCGr_G
Cuts at: 1870 1943 4139 4524 5373 5401 5543 1870
Size: 73 2196 385 849 28 142 4545
Fragments arranged by size:
4545 2196 849 385 142 73 28
AvrII C'CTAG_G
Cuts at: 2956 2956
Size: 8218
BamHI G'GATC_C
Cuts at: 3004 5548 5915 3004
Size: 2544 367 5307
Fragments arranged by size:
5307 2544 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2663 4154 4882 5267 5539
Size: 325 217 627 876 1491 728 385 272
Cuts at: 5539 6994 618
Size: 1455 1842
Fragments arranged by size:
1842 1491 1455 876 728 627 385 325
272 217
BanII G_rGCy'C
Cuts at: 1391 3112 4000 4973 5012 5261 5372 5489 5537
Size: 1721 888 973 39 249 111 117 48
Cuts at: 5537 1391
Size: 4072
Fragments arranged by size:
4072 1721 973 888 249 117 111 48
39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2251 2824 3356 928
Size: 971 352 573 532 5790
Fragments arranged by size:
5790 971 573 532 352
BbvCI CC'TCA_GC
Cuts at: 2293 3790 2293
Size: 1497 6721
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6409 6443 1903
Size: 34 4472 34 3678
Fragments arranged by size:
4472 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5421 5468 6110 7320 5421
Size: 47 642 1210 6319
Fragments arranged by size:
6319 1210 642 47
BclI T'GATC_A
Cuts at: 1548 3226 1548
Size: 1678 6540
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4037 6829 136
Size: 107 122 71 631 605 2365 2792 1525
Fragments arranged by size:
2792 2365 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3651 8213 3651
Size: 4562 3656
BlpI GC'TnA_GC
Cuts at: 3999 5052 5635 3999
Size: 1053 583 6582
Fragments arranged by size:
6582 1053 583
Bme1580I G_kGCm'C
Cuts at: 1790 2280 2666 3112 3862 4461 4755 5012 5175
Size: 490 386 446 750 599 294 257 163
Cuts at: 5175 5372 6279 7435 1790
Size: 197 907 1156 2573
Fragments arranged by size:
2573 1156 907 750 599 490 446 386
294 257 197 163
BmgBI CAC'GTC
Cuts at: 1318 2508 3061 3625 3732 4507 1318
Size: 1190 553 564 107 775 5029
Fragments arranged by size:
5029 1190 775 564 553 107
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2128 2139 2906
Size: 416 53 83 399 24 711 11 767
Cuts at: 2906 5088 5949 6907 442
Size: 2182 861 958 1753
Fragments arranged by size:
2182 1753 958 861 767 711 416 399
83 53 24 11
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8218
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3570 4399 3570
Size: 829 7389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2903 4116 4195 4498 4696 4811 4915 4998
Size: 1664 1213 79 303 198 115 104 83
Cuts at: 4998 6878 1239
Size: 1880 2579
Fragments arranged by size:
2579 1880 1664 1213 303 198 115 104
83 79
Bpu10I CC'TnA_GC
Cuts at: 2293 3790 2293
Size: 1497 6721
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2381 3969 6343 7208 7506 2381
Size: 1588 2374 865 298 3093
Fragments arranged by size:
3093 2374 1588 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4136 6881 882
Size: 617 2637 2745 2219
Fragments arranged by size:
2745 2637 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2823 3718 4291 6009 493
Size: 711 1619 895 573 1718 2702
Fragments arranged by size:
2702 1718 1619 895 711 573
BsaBI GATnn'nnATC
Cuts at: 8212 8212
Size: 8218
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6025 6407 7042 275
Size: 53 83 186 936 4492 382 635 1451
Fragments arranged by size:
4492 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4238 6651 7049 7323 7470 7881 4238
Size: 2413 398 274 147 411 4575
Fragments arranged by size:
4575 2413 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2519 2549 4496 4526 4691
Size: 30 165 30 1116 30 1947 30 165
Cuts at: 4691 4721 4895 4925 4928 4958 1178
Size: 30 174 30 3 30 4438
Fragments arranged by size:
4438 1947 1116 174 165 165 30 30
30 30 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 2462 4312 4375 4625 4628 4699 1186
Size: 1240 36 1850 63 250 3 71 4705
Fragments arranged by size:
4705 1850 1240 250 71 63 36 3
BseYI C'CCAG_C
Cuts at: 3725 3816 4032 4056 4075 4158 4565 4748 5156
Size: 91 216 24 19 83 407 183 408
Cuts at: 5156 5217 5332 7421 7917 3725
Size: 61 115 2089 496 4026
Fragments arranged by size:
4026 2089 496 408 407 216 183 115
91 83 61 24 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2456 2585 4139 4212 4757 4786 5336
Size: 573 720 129 1554 73 545 29 550
Cuts at: 5336 1163
Size: 4045
Fragments arranged by size:
4045 1554 720 573 550 545 129 73
29
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5006 5668 6429 7457 1540
Size: 275 186 3005 662 761 1028 2301
Fragments arranged by size:
3005 2301 1028 761 662 275 186
BsiHKAI G_wGCw'C
Cuts at: 2585 2759 3080 4000 4461 4973 5175 6279 6364
Size: 174 321 920 461 512 202 1104 85
Cuts at: 6364 7435 2585
Size: 1071 3368
Fragments arranged by size:
3368 1104 1071 920 512 461 321 202
174 85
BsmI GAATG_Cn'
Cuts at: 2804 5729 5822 2804
Size: 2925 93 5200
Fragments arranged by size:
5200 2925 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3067 3067
Size: 8218
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2280 2585 2666 2759 3080 3112 3862
Size: 399 490 305 81 93 321 32 750
Cuts at: 3862 4000 4461 4755 4973 5012 5175 5261 5372
Size: 138 461 294 218 39 163 86 111
Cuts at: 5372 5489 5537 6279 6364 7435 1391
Size: 117 48 742 85 1071 2174
Fragments arranged by size:
2174 1071 750 742 490 461 399 321
305 294 218 163 138 117 111 93
86 85 81 48 39 32
BspEI T'CCGG_A
Cuts at: 7881 7881
Size: 8218
BspHI T'CATG_A
Cuts at: 6107 7837 6107
Size: 1730 6488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2914 5573 844
Size: 2070 2659 3489
Fragments arranged by size:
3489 2659 2070
BsrBI CCG'CTC
Cuts at: 1394 1823 6105 1394
Size: 429 4282 3507
Fragments arranged by size:
4282 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6713 6887 65
Size: 1183 5465 174 1396
Fragments arranged by size:
5465 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2218 3492 4238 4992 5245 5264 6862
Size: 110 408 1274 746 754 253 19 1598
Cuts at: 6862 7049 1700
Size: 187 2869
Fragments arranged by size:
2869 1598 1274 754 746 408 253 187
110 19
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8218
BssHII G'CGCG_C
Cuts at: 1925 5303 1925
Size: 3378 4840
BssSI C'ACGA_G
Cuts at: 6278 7290 6278
Size: 1012 7206
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2575 4066 5195 794
Size: 1781 1491 1129 3817
Fragments arranged by size:
3817 1781 1491 1129
BstBI TT'CG_AA
Cuts at: 7022 7022
Size: 8218
BstEII G'GTnAC_C
Cuts at: 1184 2287 4486 1184
Size: 1103 2199 4916
Fragments arranged by size:
4916 2199 1103
BstXI CCAn_nnnn'nTGG
Cuts at: 2390 2390
Size: 8218
BstYI r'GATC_y
Cuts at: 1972 2688 3004 3651 4011 4062 5548 5915 6300
Size: 716 316 647 360 51 1486 367 385
Cuts at: 6300 6317 7769 7855 7867 8213 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1486 1452 716 647 385 367 360
346 316 86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3801 4146 4452 4572 5067 5118
Size: 552 939 1797 345 306 120 495 51
Cuts at: 5118 5161 5271 513
Size: 43 110 3460
Fragments arranged by size:
3460 1797 939 552 495 345 306 120
110 51 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7479
Fragments arranged by size:
7479 683 56
BtsI GCAGTG_nn'
Cuts at: 2496 3816 4603 4798 5238 5322 5734 6528 6548
Size: 1320 787 195 440 84 412 794 20
Cuts at: 6548 2496
Size: 4166
Fragments arranged by size:
4166 1320 794 787 440 412 195 84
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8218
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8183
DraI TTT'AAA
Cuts at: 5522 5522
Size: 8218
DraIII CAC_nnn'GTG
Cuts at: 2284 5284 5314 2284
Size: 3000 30 5188
Fragments arranged by size:
5188 3000 30
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7225 809
Size: 507 5909 1802
Fragments arranged by size:
5909 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3197 4043 4101
Size: 54 1425 50 275 18 1367 846 58
Cuts at: 4101 5003 5665 6554 8183 8
Size: 902 662 889 1629 43
Fragments arranged by size:
1629 1425 1367 902 889 846 662 275
58 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5003 5665 1537
Size: 275 3191 662 4090
Fragments arranged by size:
4090 3191 662 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4011 4325 4966 6148 1353
Size: 2658 314 641 1182 3423
Fragments arranged by size:
3423 2658 1182 641 314
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2346 4184 6805 7179 7325 387
Size: 1064 138 757 1838 2621 374 146 1280
Fragments arranged by size:
2621 1838 1280 1064 757 374 146 138
EcoICRI GAG'CTC
Cuts at: 3998 4971 3998
Size: 973 7245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2714 2888 2903 3368 3992 4116 4195
Size: 393 1082 174 15 465 624 124 79
Cuts at: 4195 4498 4696 4811 4915 4947 4998 6281 6878
Size: 303 198 115 104 32 51 1283 597
Cuts at: 6878 7665 7933 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1283 1082 787 624 597 465 393
303 268 198 174 124 115 104 79
51 32 15
EcoO109I rG'GnC_Cy
Cuts at: 3108 3109 4053 5009 5353 5369 5377 3108
Size: 1 944 956 344 16 8 5949
Fragments arranged by size:
5949 956 944 344 16 8 1
EcoRI G'AATT_C
Cuts at: 5527 5905 5527
Size: 378 7840
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2256 2322 2256
Size: 66 8152
FspI TGC'GCA
Cuts at: 6724 6724
Size: 8218
HaeII r_GCGC'y
Cuts at: 1678 3285 5448 7365 1678
Size: 1607 2163 1917 2531
Fragments arranged by size:
2531 2163 1917 1607
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3018 3050 3143 3175 3308
Size: 32 135 32 1446 32 93 32 133
Cuts at: 3308 3340 4496 4528 5395 5427 6859 6891 6933
Size: 32 1156 32 867 32 1432 32 42
Cuts at: 6933 6965 1373
Size: 32 2626
Fragments arranged by size:
2626 1446 1432 1156 867 135 133 93
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4891 5500 5562 5808 669
Size: 988 281 2953 609 62 246 3079
Fragments arranged by size:
3079 2953 988 609 281 246 62
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8218
HpaI GTT'AAC
Cuts at: 5808 5808
Size: 8218
KpnI G_GTAC'C
Cuts at: 5543 5543
Size: 8218
MfeI C'AATT_G
Cuts at: 3147 4545 5817 3147
Size: 1398 1272 5548
Fragments arranged by size:
5548 1398 1272
MluI A'CGCG_T
Cuts at: 8094 8094
Size: 8218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 2562 2823 4039 4728 7332 7516 1305
Size: 834 423 261 1216 689 2604 184 2007
Fragments arranged by size:
2604 2007 1216 834 689 423 261 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4045 4103 10
Size: 54 1425 343 2213 58 4125
Fragments arranged by size:
4125 2213 1425 343 58 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2999 3716 4115 4758 4826 6176 6535
Size: 648 1833 717 399 643 68 1350 359
Cuts at: 6535 6694 518
Size: 159 2042
Fragments arranged by size:
2042 1833 1350 717 648 643 399 359
159 68
MspA1I CmG'CkG
Cuts at: 1606 2532 2560 3766 3794 3820 4165 4446 4537
Size: 926 28 1206 28 26 345 281 91
Cuts at: 4537 4674 4953 5002 5195 5201 5634 6311 7459
Size: 137 279 49 193 6 433 677 1148
Cuts at: 7459 7704 1606
Size: 245 2120
Fragments arranged by size:
2120 1206 1148 926 677 433 345 281
279 245 193 137 91 49 28 28
26 6
NaeI GCC'GGC
Cuts at: 5247 5247
Size: 8218
NcoI C'CATG_G
Cuts at: 513 1065 2004 3801 4146 4452 5118 513
Size: 552 939 1797 345 306 666 3613
Fragments arranged by size:
3613 1797 939 666 552 345 306
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8218
NgoMIV G'CCGG_C
Cuts at: 5245 5245
Size: 8218
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8218
NotI GC'GGCC_GC
Cuts at: 5665 5665
Size: 8218
NspI r_CATG'y
Cuts at: 1132 1229 2039 3157 3475 3829 4127 4190 5576
Size: 97 810 1118 318 354 298 63 1386
Cuts at: 5576 7121 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1386 1118 810 354 318 298
97 63
PasI CC'CwG_GG
Cuts at: 1348 3163 5254 1348
Size: 1815 2091 4312
Fragments arranged by size:
4312 2091 1815
PciI A'CATG_T
Cuts at: 2035 3153 3471 7117 2035
Size: 1118 318 3646 3136
Fragments arranged by size:
3646 3136 1118 318
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3646 4165 5284 1108
Size: 2538 519 1119 4042
Fragments arranged by size:
4042 2538 1119 519
PfoI T'CCnGG_A
Cuts at: 3208 4325 3208
Size: 1117 7101
PmeI GTTT'AAAC
Cuts at: 5522 5522
Size: 8218
PmlI CAC'GTG
Cuts at: 2823 3718 4291 2823
Size: 895 573 6750
Fragments arranged by size:
6750 895 573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6287 6319 7800 7832 6287
Size: 32 1481 32 6673
Fragments arranged by size:
6673 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4053 5353 5377 4053
Size: 1300 24 6894
Fragments arranged by size:
6894 1300 24
PshAI GACnn'nnGTC
Cuts at: 1613 2255 3773 4547 1613
Size: 642 1518 774 5284
Fragments arranged by size:
5284 1518 774 642
PsiI TTA'TAA
Cuts at: 4206 5788 4206
Size: 1582 6636
PspOMI G'GGCC_C
Cuts at: 3108 5008 5368 3108
Size: 1900 360 5958
Fragments arranged by size:
5958 1900 360
PstI C_TGCA'G
Cuts at: 830 3811 3837 4478 5191 5570 830
Size: 2981 26 641 713 379 3478
Fragments arranged by size:
3478 2981 713 641 379 26
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8218
PvuII CAG'CTG
Cuts at: 2560 3766 4165 4674 2560
Size: 1206 399 509 6104
Fragments arranged by size:
6104 1206 509 399
RsrII CG'GwC_CG
Cuts at: 5071 5071
Size: 8218
SacI G_AGCT'C
Cuts at: 4000 4973 4000
Size: 973 7245
SalI G'TCGA_C
Cuts at: 1936 5560 1936
Size: 3624 4594
SanDI GG'GwC_CC
Cuts at: 4053 4053
Size: 8218
SbfI CC_TGCA'GG
Cuts at: 5570 5570
Size: 8218
ScaI AGT'ACT
Cuts at: 6466 8178 6466
Size: 1712 6506
SexAI A'CCwGG_T
Cuts at: 4018 5280 5349 4018
Size: 1262 69 6887
Fragments arranged by size:
6887 1262 69
SfcI C'TryA_G
Cuts at: 826 1045 2080 3807 3833 4474 5187 5566 6701
Size: 219 1035 1727 26 641 713 379 1135
Cuts at: 6701 7382 7573 826
Size: 681 191 1471
Fragments arranged by size:
1727 1471 1135 1035 713 681 641 379
219 191 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8218
SgrAI Cr'CCGG_yG
Cuts at: 1700 5245 1700
Size: 3545 4673
SmaI CCC'GGG
Cuts at: 1872 4526 5375 5545 1872
Size: 2654 849 170 4545
Fragments arranged by size:
4545 2654 849 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2396 3984 6322 7223 7485 820
Size: 197 926 453 1588 2338 901 262 1553
Fragments arranged by size:
2338 1588 1553 926 901 453 262 197
SnaBI TAC'GTA
Cuts at: 493 6009 493
Size: 5516 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8218
SphI G_CATG'C
Cuts at: 1132 3829 4127 4190 5576 1132
Size: 2697 298 63 1386 3774
Fragments arranged by size:
3774 2697 1386 298 63
SspI AAT'ATT
Cuts at: 5 52 3272 6142 5
Size: 47 3220 2870 2081
Fragments arranged by size:
3220 2870 2081 47
StuI AGG'CCT
Cuts at: 1517 3923 5102 1517
Size: 2406 1179 4633
Fragments arranged by size:
4633 2406 1179
StyI C'CwwG_G
Cuts at: 513 1065 2004 2956 3066 3124 3801 3910 4146
Size: 552 939 952 110 58 677 109 236
Cuts at: 4146 4452 4779 5118 5493 5657 513
Size: 306 327 339 375 164 3074
Fragments arranged by size:
3074 952 939 677 552 375 339 327
306 236 164 110 109 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2473 2718 4121 4172 4268 4474 4485
Size: 1255 893 245 1403 51 96 206 11
Cuts at: 4485 5231 6245 6262 6415 6600 7045 325
Size: 746 1014 17 153 185 445 1498
Fragments arranged by size:
1498 1403 1255 1014 893 746 445 245
206 185 153 96 51 17 11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3405 6464 8176
Size: 274 80 33 51 1443 1428 3059 1712
Cuts at: 8176 96
Size: 138
Fragments arranged by size:
3059 1712 1443 1428 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4120 4152 4892 4895 4924 4927 8178 8210 4120
Size: 32 740 3 29 3 3251 32 4128
Fragments arranged by size:
4128 3251 740 32 32 29 3 3
Tth111I GACn'n_nGTC
Cuts at: 1316 2721 1316
Size: 1405 6813
XbaI T'CTAG_A
Cuts at: 3011 5554 3011
Size: 2543 5675
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3336 3808 4786 4858 5269 3336
Size: 472 978 72 411 6285
Fragments arranged by size:
6285 978 472 411 72
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8218
XmaI C'CCGG_G
Cuts at: 1870 4524 5373 5543 1870
Size: 2654 849 170 4545
Fragments arranged by size:
4545 2654 849 170
XmnI GAAnn'nnTTC
Cuts at: 6347 6347
Size: 8218
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6026 276
Size: 53 83 186 936 4492 2468
Fragments arranged by size:
4492 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI SacII SapI SfiI SfoI
SrfI SwaI