(Circular) (Six-base) MAPSORT of: pFN21AE3380.seq Check: 6659 from: 1 to: 8101
pFN21AE3380 8101 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5911 278
Size: 53 83 186 936 4375 2468
Fragments arranged by size:
4375 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5444 1937
Size: 3507 4594
Acc65I G'GTAC_C
Cuts at: 4159 4223 5422 4159
Size: 64 1199 6838
Fragments arranged by size:
6838 1199 64
AclI AA'CG_TT
Cuts at: 1563 6228 6601 1563
Size: 4665 373 3063
Fragments arranged by size:
4665 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2105 2445 2826 3263 3400 3523 4124 4640
Size: 473 340 381 437 137 123 601 516
Cuts at: 4640 4689 6164 7548 7816 1632
Size: 49 1475 1384 268 1917
Fragments arranged by size:
1917 1475 1384 601 516 473 437 381
340 268 137 123 49
AflII C'TTAA_G
Cuts at: 820 1017 2759 820
Size: 197 1742 6162
Fragments arranged by size:
6162 1742 197
AflIII A'CryG_T
Cuts at: 1203 4464 7000 7977 1203
Size: 3261 2536 977 1327
Fragments arranged by size:
3261 2536 1327 977
AgeI A'CCGG_T
Cuts at: 6932 6932
Size: 8101
AhdI GACnn_n'nnGTC
Cuts at: 6830 6830
Size: 8101
AleI CACnn'nnGTG
Cuts at: 1166 2240 4330 1166
Size: 1074 2090 4937
Fragments arranged by size:
4937 2090 1074
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8101
AlwNI CAG_nnn'CTG
Cuts at: 5844 7416 5844
Size: 1572 6529
ApaLI G'TGCA_C
Cuts at: 6158 7314 6158
Size: 1156 6945
ApoI r'AATT_y
Cuts at: 1493 2810 2889 3893 4189 4195 4553 5183 5410
Size: 1317 79 1004 296 6 358 630 227
Cuts at: 5410 5636 5788 7897 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1317 1004 630 358 296 227
226 152 79 6
AseI AT'TA_AT
Cuts at: 160 1032 3107 4784 6655 160
Size: 872 2075 1677 1871 1606
Fragments arranged by size:
2075 1871 1677 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8101
AvaI C'yCGr_G
Cuts at: 1870 1943 2415 5426 1870
Size: 73 472 3011 4545
Fragments arranged by size:
4545 3011 472 73
AvrII C'CTAG_G
Cuts at: 4492 4492
Size: 8101
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2228 2261 2747 2780 2228
Size: 33 486 33 7549
Fragments arranged by size:
7549 486 33 33
BamHI G'GATC_C
Cuts at: 4522 5431 5798 4522
Size: 909 367 6825
Fragments arranged by size:
6825 909 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4159 4223 4487 4505 5422
Size: 325 217 627 2372 64 264 18 917
Cuts at: 5422 6877 618
Size: 1455 1842
Fragments arranged by size:
2372 1842 1455 917 627 325 264 217
64 18
BanII G_rGCy'C
Cuts at: 1391 5420 1391
Size: 4029 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2299 3529 4065 4073 4521 928
Size: 971 400 1230 536 8 448 4508
Fragments arranged by size:
4508 1230 971 536 448 400 8
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6292 6326 1903
Size: 34 4355 34 3678
Fragments arranged by size:
4355 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2301 5993 7203 2301
Size: 3692 1210 3199
Fragments arranged by size:
3692 3199 1210
BclI T'GATC_A
Cuts at: 1548 5304 1548
Size: 3756 4345
BfrBI ATG'CAT
Cuts at: 3988 4173 3988
Size: 185 7916
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6712 136
Size: 107 122 71 631 605 5040 1525
Fragments arranged by size:
5040 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3430 4414 5295 8096 3430
Size: 984 881 2801 3435
Fragments arranged by size:
3435 2801 984 881
BlpI GC'TnA_GC
Cuts at: 5518 5518
Size: 8101
Bme1580I G_kGCm'C
Cuts at: 1790 4492 6162 7318 1790
Size: 2702 1670 1156 2573
Fragments arranged by size:
2702 2573 1670 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8101
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4638 5832 6790
Size: 416 53 83 399 24 3221 1194 958
Cuts at: 6790 442
Size: 1753
Fragments arranged by size:
3221 1753 1194 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8101
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3609 3609
Size: 8101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3156 4419 5055 5418 6761 1239
Size: 1917 1263 636 363 1343 2579
Fragments arranged by size:
2579 1917 1343 1263 636 363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6226 7091 7389 6226
Size: 865 298 6938
Fragments arranged by size:
6938 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6764 882
Size: 617 5265 2219
Fragments arranged by size:
5265 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5892 493
Size: 711 4688 2702
Fragments arranged by size:
4688 2702 711
BsaBI GATnn'nnATC
Cuts at: 3141 3912 4311 8095 3141
Size: 771 399 3784 3147
Fragments arranged by size:
3784 3147 771 399
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5908 6290 6925 275
Size: 53 83 186 936 4375 382 635 1451
Fragments arranged by size:
4375 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6534 6932 7206 7353 7764 6534
Size: 398 274 147 411 6871
Fragments arranged by size:
6871 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3837 3867 4207 4237 1178
Size: 30 165 30 2434 30 340 30 5042
Fragments arranged by size:
5042 2434 340 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2421 2864 4266 1186
Size: 1235 443 1402 5021
Fragments arranged by size:
5021 1402 1235 443
BseYI C'CCAG_C
Cuts at: 7304 7800 7304
Size: 496 7605
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4308 1163
Size: 573 2572 4956
Fragments arranged by size:
4956 2572 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5551 6312 7340 1540
Size: 275 186 3550 761 1028 2301
Fragments arranged by size:
3550 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4273 4677 6162 6247 7318 4273
Size: 404 1485 85 1071 5056
Fragments arranged by size:
5056 1485 1071 404 85
BsiWI C'GTAC_G
Cuts at: 3691 3691
Size: 8101
BsmI GAATG_Cn'
Cuts at: 4175 5321 5612 5705 4175
Size: 1146 291 93 6571
Fragments arranged by size:
6571 1146 291 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3855 3855
Size: 8101
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4273 4492 4677 5420 6162 6247 7318
Size: 399 2483 219 185 743 742 85 1071
Cuts at: 7318 1391
Size: 2174
Fragments arranged by size:
2483 2174 1071 743 742 399 219 185
85
BspEI T'CCGG_A
Cuts at: 7764 7764
Size: 8101
BspHI T'CATG_A
Cuts at: 2518 5307 5990 7720 2518
Size: 2789 683 1730 2899
Fragments arranged by size:
2899 2789 1730 683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3242 5456 844
Size: 2398 2214 3489
Fragments arranged by size:
3489 2398 2214
BsrBI CCG'CTC
Cuts at: 1394 1823 5988 1394
Size: 429 4165 3507
Fragments arranged by size:
4165 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5209 6596 6770 65
Size: 1183 3961 1387 174 1396
Fragments arranged by size:
3961 1396 1387 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4602 6745 6932 1700
Size: 110 2792 2143 187 2869
Fragments arranged by size:
2869 2792 2143 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8101
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8101
BssSI C'ACGA_G
Cuts at: 6161 7173 6161
Size: 1012 7089
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8101
BstBI TT'CG_AA
Cuts at: 6905 6905
Size: 8101
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8101
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2144 2011
Size: 133 7968
BstYI r'GATC_y
Cuts at: 1972 2110 2283 2655 3430 4414 4522 4930 5295
Size: 138 173 372 775 984 108 408 365
Cuts at: 5295 5356 5431 5798 6183 6200 7652 7738 7750
Size: 61 75 367 385 17 1452 86 12
Cuts at: 7750 8096 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 984 775 408 385 372 367
365 346 173 138 108 86 75 61
17 12
Bsu36I CC'TnA_GG
Cuts at: 3291 3291
Size: 8101
BtgI C'CryG_G
Cuts at: 513 1065 2004 4154 513
Size: 552 939 2150 4460
Fragments arranged by size:
4460 2150 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7362
Fragments arranged by size:
7362 683 56
BtsI GCAGTG_nn'
Cuts at: 2386 2556 2739 4252 5617 6411 6431 2386
Size: 170 183 1513 1365 794 20 4056
Fragments arranged by size:
4056 1513 1365 794 183 170 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8101
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3379 3414 529
Size: 35 2815 35 5216
Fragments arranged by size:
5216 2815 35 35
DraI TTT'AAA
Cuts at: 3685 3744 3837 4194 5405 3685
Size: 59 93 357 1211 6381
Fragments arranged by size:
6381 1211 357 93 59
DraIII CAC_nnn'GTG
Cuts at: 4233 4233
Size: 8101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7108 809
Size: 507 5792 1802
Fragments arranged by size:
5792 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2598 3658 5548
Size: 54 1425 50 275 18 768 1060 1890
Cuts at: 5548 6437 8066 8
Size: 889 1629 43
Fragments arranged by size:
1890 1629 1425 1060 889 768 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5548 1537
Size: 275 3736 4090
Fragments arranged by size:
4090 3736 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2059 2439 3378 3691 4533 4665 5156 5370
Size: 706 380 939 313 842 132 491 214
Cuts at: 5370 6031 1353
Size: 661 3423
Fragments arranged by size:
3423 939 842 706 661 491 380 313
214 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6688 7062 7208 387
Size: 1064 138 5099 374 146 1280
Fragments arranged by size:
5099 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2445 2826 3156 3263 3400 3523
Size: 393 473 340 381 330 107 137 123
Cuts at: 3523 4124 4419 4640 4689 5055 5418 6164 6761
Size: 601 295 221 49 366 363 746 597
Cuts at: 6761 7548 7816 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 746 601 597 473 393 381
366 363 340 330 295 268 221 137
123 107 49
EcoNI CCTnn'n_nnAGG
Cuts at: 5350 5350
Size: 8101
EcoRI G'AATT_C
Cuts at: 2889 5410 5788 2889
Size: 2521 378 5202
Fragments arranged by size:
5202 2521 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2450 2555 2647 2856 3105 3430 2450
Size: 105 92 209 249 325 7121
Fragments arranged by size:
7121 325 249 209 105 92
FspI TGC'GCA
Cuts at: 6607 6607
Size: 8101
HaeII r_GCGC'y
Cuts at: 1678 7248 1678
Size: 5570 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2275 2307 2640
Size: 32 135 32 458 32 213 32 333
Cuts at: 2640 2672 3324 3356 3837 3869 4060 4092 5155
Size: 32 652 32 481 32 191 32 1063
Cuts at: 5155 5187 5208 5240 6742 6774 6816 6848 1373
Size: 32 21 32 1502 32 42 32 2626
Fragments arranged by size:
2626 1502 1063 652 481 458 333 213
191 135 42 32 32 32 32 32
32 32 32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 2325 3719 4995 5445 5691 669
Size: 988 281 387 1394 1276 450 246 3079
Fragments arranged by size:
3079 1394 1276 988 450 387 281 246
HindIII A'AGCT_T
Cuts at: 748 2223 3087 3812 4652 748
Size: 1475 864 725 840 4197
Fragments arranged by size:
4197 1475 864 840 725
HpaI GTT'AAC
Cuts at: 4995 5691 4995
Size: 696 7405
KpnI G_GTAC'C
Cuts at: 4163 4227 5426 4163
Size: 64 1199 6838
Fragments arranged by size:
6838 1199 64
MfeI C'AATT_G
Cuts at: 5700 5700
Size: 8101
MluI A'CGCG_T
Cuts at: 7977 7977
Size: 8101
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7215 7399 1305
Size: 5910 184 2007
Fragments arranged by size:
5910 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2600 3660 10
Size: 54 1425 343 768 1060 4451
Fragments arranged by size:
4451 1425 1060 768 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2240 2316 4020 4168 4330 6059
Size: 648 843 231 76 1704 148 162 1729
Cuts at: 6059 6418 6577 518
Size: 359 159 2042
Fragments arranged by size:
2042 1729 1704 843 648 359 231 162
159 148 76
MspA1I CmG'CkG
Cuts at: 1606 5180 5517 6194 7342 7587 1606
Size: 3574 337 677 1148 245 2120
Fragments arranged by size:
3574 2120 1148 677 337 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4154 513
Size: 552 939 2150 4460
Fragments arranged by size:
4460 2150 939 552
NdeI CA'TA_TG
Cuts at: 387 3597 4804 387
Size: 3210 1207 3684
Fragments arranged by size:
3684 3210 1207
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8101
NotI GC'GGCC_GC
Cuts at: 5548 5548
Size: 8101
NsiI A_TGCA'T
Cuts at: 3990 4175 3990
Size: 185 7916
NspI r_CATG'y
Cuts at: 1132 1229 4468 5459 7004 1132
Size: 97 3239 991 1545 2229
Fragments arranged by size:
3239 2229 1545 991 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8101
PciI A'CATG_T
Cuts at: 4464 7000 4464
Size: 2536 5565
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4233 5061 1108
Size: 3125 828 4148
Fragments arranged by size:
4148 3125 828
PmeI GTTT'AAAC
Cuts at: 5405 5405
Size: 8101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6170 6202 7683 7715 6170
Size: 32 1481 32 6556
Fragments arranged by size:
6556 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3714 1613
Size: 2101 6000
PsiI TTA'TAA
Cuts at: 2771 2870 4355 5671 2771
Size: 99 1485 1316 5201
Fragments arranged by size:
5201 1485 1316 99
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2928 2960 2928
Size: 32 8069
PstI C_TGCA'G
Cuts at: 830 2790 3136 4247 4869 5453 830
Size: 1960 346 1111 622 584 3478
Fragments arranged by size:
3478 1960 1111 622 584 346
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8101
SalI G'TCGA_C
Cuts at: 1936 5443 1936
Size: 3507 4594
SapI GCTCTTCn'nnn_
Cuts at: 4665 5370 4665
Size: 705 7396
SbfI CC_TGCA'GG
Cuts at: 5453 5453
Size: 8101
ScaI AGT'ACT
Cuts at: 4666 6349 8061 4666
Size: 1683 1712 4706
Fragments arranged by size:
4706 1712 1683
SexAI A'CCwGG_T
Cuts at: 5041 5041
Size: 8101
SfcI C'TryA_G
Cuts at: 826 1045 2344 2786 3132 4243 4559 4616 4718
Size: 219 1299 442 346 1111 316 57 102
Cuts at: 4718 4756 4865 5449 6584 7265 7456 826
Size: 38 109 584 1135 681 191 1471
Fragments arranged by size:
1471 1299 1135 1111 681 584 442 346
316 219 191 109 102 57 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8101
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8101
SmaI CCC'GGG
Cuts at: 1872 5428 1872
Size: 3556 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2415 2759 6205 7106 7368 820
Size: 197 926 472 344 3446 901 262 1553
Fragments arranged by size:
3446 1553 926 901 472 344 262 197
SnaBI TAC'GTA
Cuts at: 493 5892 493
Size: 5399 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8101
SphI G_CATG'C
Cuts at: 1132 5459 1132
Size: 4327 3774
SspI AAT'ATT
Cuts at: 5 52 2971 3308 3871 4039 4796 6025 5
Size: 47 2919 337 563 168 757 1229 2081
Fragments arranged by size:
2919 2081 1229 757 563 337 168 47
StuI AGG'CCT
Cuts at: 1517 3589 4390 1517
Size: 2072 801 5228
Fragments arranged by size:
5228 2072 801
StyI C'CwwG_G
Cuts at: 513 1065 2004 2143 3655 3661 4154 4492 5540
Size: 552 939 139 1512 6 493 338 1048
Cuts at: 5540 513
Size: 3074
Fragments arranged by size:
3074 1512 1048 939 552 493 338 139
6
SwaI ATTT'AAAT
Cuts at: 4194 4194
Size: 8101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2311 6128 6145 6298 6483 6928 325
Size: 1255 731 3817 17 153 185 445 1498
Fragments arranged by size:
3817 1498 1255 731 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2338 3274 3387
Size: 274 80 33 51 1443 361 936 113
Cuts at: 3387 4664 4740 4869 6347 8059 96
Size: 1277 76 129 1478 1712 138
Fragments arranged by size:
1712 1478 1443 1277 936 361 274 138
129 113 80 76 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4012 4044 8061 8093 4012
Size: 32 4017 32 4020
Fragments arranged by size:
4020 4017 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2447 1316
Size: 1131 6970
XbaI T'CTAG_A
Cuts at: 3994 5437 3994
Size: 1443 6658
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3044 3044
Size: 8101
XhoI C'TCGA_G
Cuts at: 1943 2415 1943
Size: 472 7629
XmaI C'CCGG_G
Cuts at: 1870 5426 1870
Size: 3556 4545
XmnI GAAnn'nnTTC
Cuts at: 3413 3459 3772 6230 3413
Size: 46 313 2458 5284
Fragments arranged by size:
5284 2458 313 46
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5909 276
Size: 53 83 186 936 4375 2468
Fragments arranged by size:
4375 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI Bpu10I
BstZ17I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SfiI SfoI
SrfI