Restriction map for
pFN21AE3379
(Circular) (Six-base) MAPSORT of: pFN21AE3379.seq Check: 495 from: 1 to: 8095
pFN21AE3379 8095 bp
With 182 enzymes: SgfI *
February 28, 2012 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5370 5370
Size: 8095
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2954 5241 5905 278
Size: 53 83 186 936 1418 2287 664 2468
Fragments arranged by size:
2468 2287 1418 936 664 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5438 1937
Size: 3501 4594
Acc65I G'GTAC_C
Cuts at: 4377 5416 4377
Size: 1039 7056
AclI AA'CG_TT
Cuts at: 1563 6222 6595 1563
Size: 4659 373 3063
Fragments arranged by size:
4659 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2489 4403 4712 4799 5066 6158 7542 7810
Size: 857 1914 309 87 267 1092 1384 268
Cuts at: 7810 1632
Size: 1917
Fragments arranged by size:
1917 1914 1384 1092 857 309 268 267
87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7898
AflIII A'CryG_T
Cuts at: 1203 3045 4540 6994 7971 1203
Size: 1842 1495 2454 977 1327
Fragments arranged by size:
2454 1842 1495 1327 977
AgeI A'CCGG_T
Cuts at: 6926 6926
Size: 8095
AhdI GACnn_n'nnGTC
Cuts at: 2511 3926 5218 6824 2511
Size: 1415 1292 1606 3782
Fragments arranged by size:
3782 1606 1415 1292
AleI CACnn'nnGTG
Cuts at: 1166 2501 1166
Size: 1335 6760
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5298 1184
Size: 4114 3981
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4025 4057 4813 4845 4025
Size: 32 756 32 7275
Fragments arranged by size:
7275 756 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2888 3406 3673 4423 4969 5838 7410 2888
Size: 518 267 750 546 869 1572 3573
Fragments arranged by size:
3573 1572 869 750 546 518 267
ApaI G_GGCC'C
Cuts at: 2091 3425 3973 4162 4901 5272 2091
Size: 1334 548 189 739 371 4914
Fragments arranged by size:
4914 1334 739 548 371 189
ApaLI G'TGCA_C
Cuts at: 6152 7308 6152
Size: 1156 6939
ApoI r'AATT_y
Cuts at: 1493 4464 5404 5630 5782 7891 1493
Size: 2971 940 226 152 2109 1697
Fragments arranged by size:
2971 2109 1697 940 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6649 160
Size: 872 5617 1606
Fragments arranged by size:
5617 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8095
AvaI C'yCGr_G
Cuts at: 1870 1943 2925 3651 3965 4325 5420 1870
Size: 73 982 726 314 360 1095 4545
Fragments arranged by size:
4545 1095 982 726 360 314 73
BamHI G'GATC_C
Cuts at: 4938 5425 5792 4938
Size: 487 367 7241
Fragments arranged by size:
7241 487 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2782 3053 3556 4377 4514
Size: 325 217 627 995 271 503 821 137
Cuts at: 4514 5096 5416 6871 618
Size: 582 320 1455 1842
Fragments arranged by size:
1842 1455 995 821 627 582 503 325
320 271 217 137
BanII G_rGCy'C
Cuts at: 1391 2052 2091 2196 2493 2724 3063 3425 3570
Size: 661 39 105 297 231 339 362 145
Cuts at: 3570 3758 3787 3973 4162 4203 4321 4491 4857
Size: 188 29 186 189 41 118 170 366
Cuts at: 4857 4901 5272 5414 1391
Size: 44 371 142 4072
Fragments arranged by size:
4072 661 371 366 362 339 297 231
189 188 186 170 145 142 118 105
44 41 39 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2311 3340 4797 4845 928
Size: 971 412 1029 1457 48 4178
Fragments arranged by size:
4178 1457 1029 971 412 48
BbvCI CC'TCA_GC
Cuts at: 3198 3198
Size: 8095
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6286 6320 1903
Size: 34 50 34 4265 34 3678
Fragments arranged by size:
4265 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5987 7197 5987
Size: 1210 6885
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8095
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2719 3075 3939
Size: 107 122 71 631 605 1047 356 864
Cuts at: 3939 3992 4448 4457 4562 6706 136
Size: 53 456 9 105 2144 1525
Fragments arranged by size:
2144 1525 1047 864 631 605 456 356
122 107 105 71 53 9
BglII A'GATC_T
Cuts at: 8090 8090
Size: 8095
BlpI GC'TnA_GC
Cuts at: 2487 5150 5512 2487
Size: 2663 362 5070
Fragments arranged by size:
5070 2663 362
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2787 3425 3973 4162 4517 4901 5272
Size: 301 696 638 548 189 355 384 371
Cuts at: 5272 6156 7312 1790
Size: 884 1156 2573
Fragments arranged by size:
2573 1156 884 696 638 548 384 371
355 301 189
BmgBI CAC'GTC
Cuts at: 1318 4057 5179 1318
Size: 2739 1122 4234
Fragments arranged by size:
4234 2739 1122
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2805 3172 3328
Size: 416 53 83 399 24 1388 367 156
Cuts at: 3328 4556 5826 6784 442
Size: 1228 1270 958 1753
Fragments arranged by size:
1753 1388 1270 1228 958 416 399 367
156 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8095
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2608 3589 4340 5032 5214 5319 2215
Size: 393 981 751 692 182 105 4991
Fragments arranged by size:
4991 981 751 692 393 182 105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2196 2357 2626 2630 2740 2829 2997
Size: 890 67 161 269 4 110 89 168
Cuts at: 2997 3134 3158 3899 4277 4445 4904 6755 1239
Size: 137 24 741 378 168 459 1851 2579
Fragments arranged by size:
2579 1851 890 741 459 378 269 168
168 161 137 110 89 67 24 4
Bpu10I CC'TnA_GC
Cuts at: 2345 3009 3198 3674 3757 5144 2345
Size: 664 189 476 83 1387 5296
Fragments arranged by size:
5296 1387 664 476 189 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4690 6220 7085 7383 4690
Size: 1530 865 298 5402
Fragments arranged by size:
5402 1530 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2267 2758 2989 3466 4709 6758 882
Size: 617 768 491 231 477 1243 2049 2219
Fragments arranged by size:
2219 2049 1243 768 617 491 477 231
BsaAI yAC'GTr
Cuts at: 493 1204 3048 3546 3736 4477 5886 493
Size: 711 1844 498 190 741 1409 2702
Fragments arranged by size:
2702 1844 1409 741 711 498 190
BsaBI GATnn'nnATC
Cuts at: 8089 8089
Size: 8095
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2951 3687 3894 5238
Size: 53 83 186 936 1418 736 207 1344
Cuts at: 5238 5330 5902 6284 6919 275
Size: 92 572 382 635 1451
Fragments arranged by size:
1451 1418 1344 936 736 635 572 382
207 186 92 83 53
BsaWI w'CCGG_w
Cuts at: 5209 6528 6926 7200 7347 7758 5209
Size: 1319 398 274 147 411 5546
Fragments arranged by size:
5546 1319 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4025 4055 4473 4503 1178
Size: 30 165 30 2622 30 418 30 4770
Fragments arranged by size:
4770 2622 418 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2465 3133 3524 3623 3626 3785 4469 4754
Size: 1279 668 391 99 3 159 684 285
Cuts at: 4754 1186
Size: 4527
Fragments arranged by size:
4527 1279 684 668 391 285 159 99
3
BseYI C'CCAG_C
Cuts at: 2030 2331 2897 3259 3293 3937 4003 4130 4280
Size: 301 566 362 34 644 66 127 150
Cuts at: 4280 4847 4894 5040 5271 7298 7794 2030
Size: 567 47 146 231 2027 496 2331
Fragments arranged by size:
2331 2027 644 567 566 496 362 301
231 150 146 127 66 47 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3122 3521 3966 4418 4829 1163
Size: 573 1386 399 445 452 411 4429
Fragments arranged by size:
4429 1386 573 452 445 411 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3233 3444 4498 5545 6306 7334
Size: 275 186 1232 211 1054 1047 761 1028
Cuts at: 7334 1540
Size: 2301
Fragments arranged by size:
2301 1232 1054 1047 1028 761 275 211
186
BsiHKAI G_wGCw'C
Cuts at: 2196 2946 3225 3758 3774 4203 4491 4857 6156
Size: 750 279 533 16 429 288 366 1299
Cuts at: 6156 6241 7312 2196
Size: 85 1071 2979
Fragments arranged by size:
2979 1299 1071 750 533 429 366 288
279 85 16
BsmI GAATG_Cn'
Cuts at: 5606 5699 5606
Size: 93 8002
BsmBI CGTCTCn'nnnn_
Cuts at: 5306 5306
Size: 8095
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2091 2196 2493 2724 2787 2946
Size: 399 262 39 105 297 231 63 159
Cuts at: 2946 3063 3225 3425 3570 3758 3774 3787 3973
Size: 117 162 200 145 188 16 13 186
Cuts at: 3973 4162 4203 4321 4491 4517 4857 4901 5272
Size: 189 41 118 170 26 340 44 371
Cuts at: 5272 5414 6156 6241 7312 1391
Size: 142 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 371 340 297 262
231 200 189 188 186 170 162 159
145 142 118 117 105 85 63 44
41 39 26 16 13
BspEI T'CCGG_A
Cuts at: 5209 7758 5209
Size: 2549 5546
BspHI T'CATG_A
Cuts at: 5984 7714 5984
Size: 1730 6365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2901 2920 5370 5450 844
Size: 2057 19 2450 80 3489
Fragments arranged by size:
3489 2450 2057 80 19
BsrBI CCG'CTC
Cuts at: 1394 1823 3033 3142 5982 1394
Size: 429 1210 109 2840 3507
Fragments arranged by size:
3507 2840 1210 429 109
BsrDI GCAATG_nn'
Cuts at: 65 1248 3364 4151 6590 6764 65
Size: 1183 2116 787 2439 174 1396
Fragments arranged by size:
2439 2116 1396 1183 787 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2850 3013 3225 3324 3553 3559 3593
Size: 110 1040 163 212 99 229 6 34
Cuts at: 3593 3804 6739 6926 1700
Size: 211 2935 187 2869
Fragments arranged by size:
2935 2869 1040 229 212 211 187 163
110 99 34 6
BsrGI T'GTAC_A
Cuts at: 96 5157 96
Size: 5061 3034
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8095
BssSI C'ACGA_G
Cuts at: 3243 3434 4445 4631 5199 6155 7167 3243
Size: 191 1011 186 568 956 1012 4171
Fragments arranged by size:
4171 1012 1011 956 568 191 186
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3406 4366 794
Size: 2612 960 4523
Fragments arranged by size:
4523 2612 960
BstBI TT'CG_AA
Cuts at: 6899 6899
Size: 8095
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8095
BstXI CCAn_nnnn'nTGG
Cuts at: 2083 2083
Size: 8095
BstYI r'GATC_y
Cuts at: 1972 3094 3403 4938 5425 5792 6177 6194 7646
Size: 1122 309 1535 487 367 385 17 1452
Cuts at: 7646 7732 7744 8090 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1535 1452 1122 487 385 367 346
309 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2673 2673
Size: 8095
BtgI C'CryG_G
Cuts at: 513 1065 2076 2716 2773 2827 3714 4100 513
Size: 552 1011 640 57 54 887 386 4508
Fragments arranged by size:
4508 1011 887 640 552 386 57 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2139 4085 5336 487
Size: 683 56 913 1946 1251 3246
Fragments arranged by size:
3246 1946 1251 913 683 56
BtsI GCAGTG_nn'
Cuts at: 2561 3035 3306 3858 4403 4435 5611 6405 6425
Size: 474 271 552 545 32 1176 794 20
Cuts at: 6425 2561
Size: 4231
Fragments arranged by size:
4231 1176 794 552 545 474 271 32
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8095
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8060
DraI TTT'AAA
Cuts at: 5399 5399
Size: 8095
DraIII CAC_nnn'GTG
Cuts at: 2069 2069
Size: 8095
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2315 5012 7102 809
Size: 507 999 2697 2090 1802
Fragments arranged by size:
2697 2090 1802 999 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2596 2852 3150
Size: 54 1425 50 275 18 766 256 298
Cuts at: 3150 3230 3322 3441 3591 3806 3984 4074 4495
Size: 80 92 119 150 215 178 90 421
Cuts at: 4495 5542 6431 8060 8
Size: 1047 889 1629 43
Fragments arranged by size:
1629 1425 1047 889 766 421 298 275
256 215 178 150 119 92 90 80
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3230 3441 4495 5542 1537
Size: 275 1418 211 1054 1047 4090
Fragments arranged by size:
4090 1418 1054 1047 275 211
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 2265 3416 3464 4229 6025 1353
Size: 825 87 1151 48 765 1796 3423
Fragments arranged by size:
3423 1796 1151 825 765 87 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3578 6682 7056 7202 387
Size: 1064 138 1989 3104 374 146 1280
Fragments arranged by size:
3104 1989 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2194 3756 4201 4489 4855 2194
Size: 1562 445 288 366 5434
Fragments arranged by size:
5434 1562 445 366 288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2196 2357 2489 2626 2630 2740
Size: 393 497 67 161 132 137 4 110
Cuts at: 2740 2829 2997 3134 3158 3899 4277 4403 4445
Size: 89 168 137 24 741 378 126 42
Cuts at: 4445 4712 4799 4904 5066 6158 6755 7542 7810
Size: 267 87 105 162 1092 597 787 268
Cuts at: 7810 1239
Size: 1524
Fragments arranged by size:
1524 1092 787 741 597 497 393 378
268 267 168 162 161 137 137 132
126 110 105 89 87 67 42 24
4
EcoNI CCTnn'n_nnAGG
Cuts at: 2348 3086 3197 3677 4031 4905 2348
Size: 738 111 480 354 874 5538
Fragments arranged by size:
5538 874 738 480 354 111
EcoO109I rG'GnC_Cy
Cuts at: 2034 2087 2088 2341 2678 3871 3969 3970 4062
Size: 53 1 253 337 1193 98 1 92
Cuts at: 4062 4158 4159 4239 4870 4898 4910 5116 5268
Size: 96 1 80 631 28 12 206 152
Cuts at: 5268 2034
Size: 4861
Fragments arranged by size:
4861 1193 631 337 253 206 152 98
96 92 80 53 28 12 1 1
1
EcoRI G'AATT_C
Cuts at: 5404 5782 5404
Size: 378 7717
FseI GG_CCGG'CC
Cuts at: 3597 3597
Size: 8095
FspI TGC'GCA
Cuts at: 6601 6601
Size: 8095
HaeII r_GCGC'y
Cuts at: 1678 4688 7242 1678
Size: 3010 2554 2531
Fragments arranged by size:
3010 2554 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2497 2529 2808 2840 3912
Size: 32 135 32 925 32 279 32 1072
Cuts at: 3912 3944 5204 5236 6736 6768 6810 6842 1373
Size: 32 1260 32 1500 32 42 32 2626
Fragments arranged by size:
2626 1500 1260 1072 925 279 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5439 5685 669
Size: 988 281 3501 246 3079
Fragments arranged by size:
3501 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2993 4802 5152 748
Size: 2245 1809 350 3691
Fragments arranged by size:
3691 2245 1809 350
HpaI GTT'AAC
Cuts at: 5685 5685
Size: 8095
KpnI G_GTAC'C
Cuts at: 4381 5420 4381
Size: 1039 7056
MfeI C'AATT_G
Cuts at: 5694 5694
Size: 8095
MluI A'CGCG_T
Cuts at: 7971 7971
Size: 8095
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3012 4114 7209 7393 1305
Size: 1707 1102 3095 184 2007
Fragments arranged by size:
3095 2007 1707 1102 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3152 3986 4076 10
Size: 54 1425 343 1320 834 90 4029
Fragments arranged by size:
4029 1425 1320 834 343 90 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2154 2501 3083 6053 6412 6571 518
Size: 648 988 347 582 2970 359 159 2042
Fragments arranged by size:
2970 2042 988 648 582 359 347 159
MspA1I CmG'CkG
Cuts at: 1606 2335 2486 2975 3178 4102 4308 4420 4644
Size: 729 151 489 203 924 206 112 224
Cuts at: 4644 4847 5044 5511 6188 7336 7581 1606
Size: 203 197 467 677 1148 245 2120
Fragments arranged by size:
2120 1148 924 729 677 489 467 245
224 206 203 203 197 151 112
NaeI GCC'GGC
Cuts at: 3326 3595 3326
Size: 269 7826
NcoI C'CATG_G
Cuts at: 513 1065 2076 2716 2773 3714 513
Size: 552 1011 640 57 941 4894
Fragments arranged by size:
4894 1011 941 640 552 57
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8095
NgoMIV G'CCGG_C
Cuts at: 3324 3593 3324
Size: 269 7826
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8095
NotI GC'GGCC_GC
Cuts at: 3230 5542 3230
Size: 2312 5783
NspI r_CATG'y
Cuts at: 1132 1229 2905 4544 5453 6998 1132
Size: 97 1676 1639 909 1545 2229
Fragments arranged by size:
2229 1676 1639 1545 909 97
PasI CC'CwG_GG
Cuts at: 1348 3418 1348
Size: 2070 6025
PciI A'CATG_T
Cuts at: 4540 6994 4540
Size: 2454 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8095
PfoI T'CCnGG_A
Cuts at: 2322 3798 5352 2322
Size: 1476 1554 5065
Fragments arranged by size:
5065 1554 1476
PmeI GTTT'AAAC
Cuts at: 5399 5399
Size: 8095
PmlI CAC'GTG
Cuts at: 3546 4477 3546
Size: 931 7164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 4730 4762 6164 6196 7677 7709 4025
Size: 32 673 32 1402 32 1481 32 4411
Fragments arranged by size:
4411 1481 1402 673 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2034 2678 4062 4870 2034
Size: 644 1384 808 5259
Fragments arranged by size:
5259 1384 808 644
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8095
PsiI TTA'TAA
Cuts at: 5665 5665
Size: 8095
PspOMI G'GGCC_C
Cuts at: 2087 3421 3969 4158 4897 5268 2087
Size: 1334 548 189 739 371 4914
Fragments arranged by size:
4914 1334 739 548 371 189
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5237 5269 5237
Size: 32 8063
PstI C_TGCA'G
Cuts at: 830 3042 3402 4395 4965 5447 830
Size: 2212 360 993 570 482 3478
Fragments arranged by size:
3478 2212 993 570 482 360
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8095
PvuII CAG'CTG
Cuts at: 2335 2486 2975 3178 4420 4644 4847 5044 2335
Size: 151 489 203 1242 224 203 197 5386
Fragments arranged by size:
5386 1242 489 224 203 203 197 151
RsrII CG'GwC_CG
Cuts at: 3551 3551
Size: 8095
SacI G_AGCT'C
Cuts at: 2196 3758 4203 4491 4857 2196
Size: 1562 445 288 366 5434
Fragments arranged by size:
5434 1562 445 366 288
SacII CC_GC'GG
Cuts at: 4103 4103
Size: 8095
SalI G'TCGA_C
Cuts at: 1936 5437 1936
Size: 3501 4594
SanDI GG'GwC_CC
Cuts at: 2678 2678
Size: 8095
SapI GCTCTTCn'nnn_
Cuts at: 3416 3416
Size: 8095
SbfI CC_TGCA'GG
Cuts at: 3402 5447 3402
Size: 2045 6050
ScaI AGT'ACT
Cuts at: 6343 8055 6343
Size: 1712 6383
SfcI C'TryA_G
Cuts at: 826 1045 3038 3398 4028 4391 4961 5443 6578
Size: 219 1993 360 630 363 570 482 1135
Cuts at: 6578 7259 7450 826
Size: 681 191 1471
Fragments arranged by size:
1993 1471 1135 681 630 570 482 363
360 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3939 3992 3939
Size: 53 8042
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8095
SgrAI Cr'CCGG_yG
Cuts at: 1700 3225 3559 1700
Size: 1525 334 6236
Fragments arranged by size:
6236 1525 334
SmaI CCC'GGG
Cuts at: 1872 3653 3967 5422 1872
Size: 1781 314 1455 4545
Fragments arranged by size:
4545 1781 1455 314
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4669 6199 7100 7362 820
Size: 197 926 2726 1530 901 262 1553
Fragments arranged by size:
2726 1553 1530 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5886 493
Size: 5393 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8095
SphI G_CATG'C
Cuts at: 1132 2905 5453 1132
Size: 1773 2548 3774
Fragments arranged by size:
3774 2548 1773
SspI AAT'ATT
Cuts at: 5 52 6019 5
Size: 47 5967 2081
Fragments arranged by size:
5967 2081 47
StuI AGG'CCT
Cuts at: 1517 3192 4556 1517
Size: 1675 1364 5056
Fragments arranged by size:
5056 1675 1364
StyI C'CwwG_G
Cuts at: 513 1065 2053 2076 2716 2773 3184 3216 3714
Size: 552 988 23 640 57 411 32 498
Cuts at: 3714 4226 4557 5052 5534 513
Size: 512 331 495 482 3074
Fragments arranged by size:
3074 988 640 552 512 498 495 482
411 331 57 32 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2975 4110 5084 6122 6139 6292 6477
Size: 1255 1395 1135 974 1038 17 153 185
Cuts at: 6477 6922 325
Size: 445 1498
Fragments arranged by size:
1498 1395 1255 1135 1038 974 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4543 5157 5244
Size: 274 80 33 51 1443 2566 614 87
Cuts at: 5244 6341 8053 96
Size: 1097 1712 138
Fragments arranged by size:
2566 1712 1443 1097 614 274 138 87
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2936 2968 4791 4823 8055 8087 2936
Size: 32 1823 32 3232 32 2944
Fragments arranged by size:
3232 2944 1823 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3344 1316
Size: 2028 6067
XbaI T'CTAG_A
Cuts at: 5431 5431
Size: 8095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2080 2011
Size: 69 8026
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8095
XmaI C'CCGG_G
Cuts at: 1870 3651 3965 5420 1870
Size: 1781 314 1455 4545
Fragments arranged by size:
4545 1781 1455 314
XmnI GAAnn'nnTTC
Cuts at: 6224 6224
Size: 8095
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2952 5239 5903 276
Size: 53 83 186 936 1418 2287 664 2468
Fragments arranged by size:
2468 2287 1418 936 664 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
EcoRV FalI FspAI KasI NarI NruI NsiI PacI
PspXI SexAI SfoI SrfI SwaI