Restriction map for
pFN21AE3378
(Circular) (Six-base) MAPSORT of: pFN21AE3378.seq Check: 5955 from: 1 to: 8095
pFN21AE3378 8095 bp
With 182 enzymes: SgfI *
March 28, 2012 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5905 278
Size: 53 83 186 936 4369 2468
Fragments arranged by size:
4369 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3976 5438 1937
Size: 2039 1462 4594
Fragments arranged by size:
4594 2039 1462
Acc65I G'GTAC_C
Cuts at: 5416 5416
Size: 8095
AclI AA'CG_TT
Cuts at: 1563 6222 6595 1563
Size: 4659 373 3063
Fragments arranged by size:
4659 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2283 2357 3666 4299 4546 4697 4782 4962
Size: 651 74 1309 633 247 151 85 180
Cuts at: 4962 6158 7542 7810 1632
Size: 1196 1384 268 1917
Fragments arranged by size:
1917 1384 1309 1196 651 633 268 247
180 151 85 74
AfeI AGC'GCT
Cuts at: 2831 2831
Size: 8095
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7898
AflIII A'CryG_T
Cuts at: 1203 3940 6994 7971 1203
Size: 2737 3054 977 1327
Fragments arranged by size:
3054 2737 1327 977
AgeI A'CCGG_T
Cuts at: 6926 6926
Size: 8095
AhdI GACnn_n'nnGTC
Cuts at: 6824 6824
Size: 8095
AleI CACnn'nnGTG
Cuts at: 1166 4792 1166
Size: 3626 4469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2475 1184
Size: 1291 6804
AlwNI CAG_nnn'CTG
Cuts at: 2468 3464 5838 7410 2468
Size: 996 2374 1572 3153
Fragments arranged by size:
3153 2374 1572 996
ApaLI G'TGCA_C
Cuts at: 6152 7308 6152
Size: 1156 6939
ApoI r'AATT_y
Cuts at: 1493 2103 2122 2213 2244 2281 2411 3014 3391
Size: 610 19 91 31 37 130 603 377
Cuts at: 3391 3923 5116 5234 5404 5630 5782 7891 1493
Size: 532 1193 118 170 226 152 2109 1697
Fragments arranged by size:
2109 1697 1193 610 603 532 377 226
170 152 130 118 91 37 31 19
AseI AT'TA_AT
Cuts at: 160 1032 2481 4385 5310 6649 160
Size: 872 1449 1904 925 1339 1606
Fragments arranged by size:
1904 1606 1449 1339 925 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8095
AvaI C'yCGr_G
Cuts at: 1870 1943 3337 5420 1870
Size: 73 1394 2083 4545
Fragments arranged by size:
4545 2083 1394 73
BamHI G'GATC_C
Cuts at: 4645 5425 5792 4645
Size: 780 367 6948
Fragments arranged by size:
6948 780 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3235 5416 6871 618
Size: 325 217 627 1448 2181 1455 1842
Fragments arranged by size:
2181 1842 1455 1448 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5414 1391
Size: 4023 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2647 928
Size: 971 748 6376
Fragments arranged by size:
6376 971 748
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2228 2262 6286 6320 1903
Size: 34 291 34 4024 34 3678
Fragments arranged by size:
4024 3678 291 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3555 4775 5987 7197 3555
Size: 1220 1212 1210 4453
Fragments arranged by size:
4453 1220 1212 1210
BclI T'GATC_A
Cuts at: 1548 5347 1548
Size: 3799 4296
BfrBI ATG'CAT
Cuts at: 5225 5225
Size: 8095
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6706 136
Size: 107 122 71 631 605 5034 1525
Fragments arranged by size:
5034 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8090 8090
Size: 8095
BlpI GC'TnA_GC
Cuts at: 2154 5512 2154
Size: 3358 4737
Bme1580I G_kGCm'C
Cuts at: 1790 6156 7312 1790
Size: 4366 1156 2573
Fragments arranged by size:
4366 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8095
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3322 3585 5826
Size: 416 53 83 399 24 1905 263 2241
Cuts at: 5826 6784 442
Size: 958 1753
Fragments arranged by size:
2241 1905 1753 958 416 399 263 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8095
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2757 2757
Size: 8095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3306 5035 6755 1239
Size: 2067 1729 1720 2579
Fragments arranged by size:
2579 2067 1729 1720
Bpu10I CC'TnA_GC
Cuts at: 2148 4990 2148
Size: 2842 5253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2709 3897 4011 4636 6220 7085 7383 2709
Size: 1188 114 625 1584 865 298 3421
Fragments arranged by size:
3421 1584 1188 865 625 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3028 6758 882
Size: 617 1529 3730 2219
Fragments arranged by size:
3730 2219 1529 617
BsaAI yAC'GTr
Cuts at: 493 1204 2854 5886 493
Size: 711 1650 3032 2702
Fragments arranged by size:
3032 2702 1650 711
BsaBI GATnn'nnATC
Cuts at: 8089 8089
Size: 8095
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5902 6284 6919 275
Size: 53 83 186 936 4369 382 635 1451
Fragments arranged by size:
4369 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6528 6926 7200 7347 7758 6528
Size: 398 274 147 411 6865
Fragments arranged by size:
6865 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2580 2610 2758 2779 2788
Size: 30 165 30 1177 30 148 21 9
Cuts at: 2788 2809 2843 2873 3463 3493 4798 4828 1178
Size: 21 34 30 590 30 1305 30 4445
Fragments arranged by size:
4445 1305 1177 590 165 148 34 30
30 30 30 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2733 3900 1186
Size: 840 707 1167 5381
Fragments arranged by size:
5381 1167 840 707
BseYI C'CCAG_C
Cuts at: 7298 7794 7298
Size: 496 7599
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2528 3680 4467 4537 4683 1163
Size: 573 792 1152 787 70 146 4575
Fragments arranged by size:
4575 1152 792 787 573 146 70
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5545 6306 7334 1540
Size: 275 186 3544 761 1028 2301
Fragments arranged by size:
3544 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6156 6241 7312 6156
Size: 85 1071 6939
Fragments arranged by size:
6939 1071 85
BsmI GAATG_Cn'
Cuts at: 3048 5225 5606 5699 3048
Size: 2177 381 93 5444
Fragments arranged by size:
5444 2177 381 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 5414 6156 6241 7312 1391
Size: 399 3624 742 85 1071 2174
Fragments arranged by size:
3624 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7758 7758
Size: 8095
BspHI T'CATG_A
Cuts at: 3072 5984 7714 3072
Size: 2912 1730 3453
Fragments arranged by size:
3453 2912 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5450 844
Size: 4606 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4690 5982 1394
Size: 429 2867 1292 3507
Fragments arranged by size:
3507 2867 1292 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6590 6764 65
Size: 1183 5342 174 1396
Fragments arranged by size:
5342 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 6739 6926 1700
Size: 110 275 4654 187 2869
Fragments arranged by size:
4654 2869 275 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8095
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8095
BssSI C'ACGA_G
Cuts at: 6155 7167 6155
Size: 1012 7083
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2468 794
Size: 1674 6421
BstBI TT'CG_AA
Cuts at: 2248 4213 4234 6899 2248
Size: 1965 21 2665 3444
Fragments arranged by size:
3444 2665 1965 21
BstEII G'GTnAC_C
Cuts at: 1184 4598 1184
Size: 3414 4681
BstYI r'GATC_y
Cuts at: 1972 2185 2437 2654 3182 4645 5425 5792 6177
Size: 213 252 217 528 1463 780 367 385
Cuts at: 6177 6194 7646 7732 7744 8090 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1463 1452 780 528 385 367 346
252 217 213 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2117 3603 2117
Size: 1486 6609
BtgI C'CryG_G
Cuts at: 513 1065 4607 513
Size: 552 3542 4001
Fragments arranged by size:
4001 3542 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4729 487
Size: 683 56 3503 3853
Fragments arranged by size:
3853 3503 683 56
BtsI GCAGTG_nn'
Cuts at: 3349 3462 5611 6405 6425 3349
Size: 113 2149 794 20 5019
Fragments arranged by size:
5019 2149 794 113 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8095
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8060
DraI TTT'AAA
Cuts at: 3922 5035 5399 3922
Size: 1113 364 6618
Fragments arranged by size:
6618 1113 364
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7102 809
Size: 507 5786 1802
Fragments arranged by size:
5786 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5542 6431 8060
Size: 54 1425 50 275 18 3712 889 1629
Cuts at: 8060 8
Size: 43
Fragments arranged by size:
3712 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5542 1537
Size: 275 3730 4090
Fragments arranged by size:
4090 3730 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 2376 3870 4215 4914 6025 1353
Size: 650 373 1494 345 699 1111 3423
Fragments arranged by size:
3423 1494 1111 699 650 373 345
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6682 7056 7202 387
Size: 1064 138 5093 374 146 1280
Fragments arranged by size:
5093 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2357 3306 3666 4299 4546 4697
Size: 393 651 74 949 360 633 247 151
Cuts at: 4697 4782 4962 5035 6158 6755 7542 7810 1239
Size: 85 180 73 1123 597 787 268 1524
Fragments arranged by size:
1524 1123 949 787 651 633 597 393
360 268 247 180 151 85 74 73
EcoNI CCTnn'n_nnAGG
Cuts at: 2757 2778 2946 5245 2757
Size: 21 168 2299 5607
Fragments arranged by size:
5607 2299 168 21
EcoO109I rG'GnC_Cy
Cuts at: 4976 4976
Size: 8095
EcoRI G'AATT_C
Cuts at: 2411 5404 5782 2411
Size: 2993 378 4724
Fragments arranged by size:
4724 2993 378
EcoRV GAT'ATC
Cuts at: 4270 4270
Size: 8095
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4244 4244
Size: 8095
FspI TGC'GCA
Cuts at: 6601 6601
Size: 8095
HaeII r_GCGC'y
Cuts at: 1678 2833 7242 1678
Size: 1155 4409 2531
Fragments arranged by size:
4409 2531 1155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2758 2790 2976 3008 5318
Size: 32 135 32 1186 32 186 32 2310
Cuts at: 5318 5350 5359 5391 6736 6768 6810 6842 1373
Size: 32 9 32 1345 32 42 32 2626
Fragments arranged by size:
2626 2310 1345 1186 186 135 42 32
32 32 32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2178 2521 5439 5685 669
Size: 988 281 240 343 2918 246 3079
Fragments arranged by size:
3079 2918 988 343 281 246 240
HindIII A'AGCT_T
Cuts at: 748 3760 4944 748
Size: 3012 1184 3899
Fragments arranged by size:
3899 3012 1184
HpaI GTT'AAC
Cuts at: 2521 5685 2521
Size: 3164 4931
KpnI G_GTAC'C
Cuts at: 5420 5420
Size: 8095
MfeI C'AATT_G
Cuts at: 2406 5040 5694 2406
Size: 2634 654 4807
Fragments arranged by size:
4807 2634 654
MluI A'CGCG_T
Cuts at: 7971 7971
Size: 8095
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3997 5316 7209 7393 1305
Size: 2692 1319 1893 184 2007
Fragments arranged by size:
2692 2007 1893 1319 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6273
Fragments arranged by size:
6273 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3084 3380 4792 6053 6412 6571 518
Size: 648 1918 296 1412 1261 359 159 2042
Fragments arranged by size:
2042 1918 1412 1261 648 359 296 159
MspA1I CmG'CkG
Cuts at: 1606 2468 5511 6188 7336 7581 1606
Size: 862 3043 677 1148 245 2120
Fragments arranged by size:
3043 2120 1148 862 677 245
NaeI GCC'GGC
Cuts at: 2087 2087
Size: 8095
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7543
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8095
NgoMIV G'CCGG_C
Cuts at: 2085 2085
Size: 8095
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8095
NotI GC'GGCC_GC
Cuts at: 5542 5542
Size: 8095
NruI TCG'CGA
Cuts at: 5379 5379
Size: 8095
NsiI A_TGCA'T
Cuts at: 5227 5227
Size: 8095
NspI r_CATG'y
Cuts at: 1132 1229 3944 5225 5453 6998 1132
Size: 97 2715 1281 228 1545 2229
Fragments arranged by size:
2715 2229 1545 1281 228 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8095
PciI A'CATG_T
Cuts at: 3940 6994 3940
Size: 3054 5041
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8095
PmeI GTTT'AAAC
Cuts at: 5399 5399
Size: 8095
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6164 6196 7677 7709 6164
Size: 32 1481 32 6550
Fragments arranged by size:
6550 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4976 4976
Size: 8095
PshAI GACnn'nnGTC
Cuts at: 1613 2647 5068 1613
Size: 1034 2421 4640
Fragments arranged by size:
4640 2421 1034
PsiI TTA'TAA
Cuts at: 5665 5665
Size: 8095
PstI C_TGCA'G
Cuts at: 830 2464 5447 830
Size: 1634 2983 3478
Fragments arranged by size:
3478 2983 1634
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8095
PvuII CAG'CTG
Cuts at: 2468 2468
Size: 8095
SalI G'TCGA_C
Cuts at: 1936 5437 1936
Size: 3501 4594
SapI GCTCTTCn'nnn_
Cuts at: 2376 3870 2376
Size: 1494 6601
SbfI CC_TGCA'GG
Cuts at: 5447 5447
Size: 8095
ScaI AGT'ACT
Cuts at: 2296 4586 6343 8055 2296
Size: 2290 1757 1712 2336
Fragments arranged by size:
2336 2290 1757 1712
SfcI C'TryA_G
Cuts at: 826 1045 2460 3527 5443 6578 7259 7450 826
Size: 219 1415 1067 1916 1135 681 191 1471
Fragments arranged by size:
1916 1471 1415 1135 1067 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8095
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8095
SmaI CCC'GGG
Cuts at: 1872 5422 1872
Size: 3550 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2724 3912 4026 4651 6199 7100
Size: 197 926 781 1188 114 625 1548 901
Cuts at: 7100 7362 820
Size: 262 1553
Fragments arranged by size:
1553 1548 1188 926 901 781 625 262
197 114
SnaBI TAC'GTA
Cuts at: 493 2854 5886 493
Size: 2361 3032 2702
Fragments arranged by size:
3032 2702 2361
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8095
SphI G_CATG'C
Cuts at: 1132 5453 1132
Size: 4321 3774
SspI AAT'ATT
Cuts at: 5 52 4504 6019 5
Size: 47 4452 1515 2081
Fragments arranged by size:
4452 2081 1515 47
StuI AGG'CCT
Cuts at: 1517 2047 1517
Size: 530 7565
StyI C'CwwG_G
Cuts at: 513 1065 3768 5534 513
Size: 552 2703 1766 3074
Fragments arranged by size:
3074 2703 1766 552
SwaI ATTT'AAAT
Cuts at: 3922 3922
Size: 8095
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2224 5115 6122 6139 6292 6477 6922
Size: 1255 644 2891 1007 17 153 185 445
Cuts at: 6922 325
Size: 1498
Fragments arranged by size:
2891 1498 1255 1007 644 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2294 3952 4584
Size: 274 80 33 51 1443 317 1658 632
Cuts at: 4584 6341 8053 96
Size: 1757 1712 138
Fragments arranged by size:
1757 1712 1658 1443 632 317 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2580 2612 2688 2720 4798 4830 8055 8087 2580
Size: 32 76 32 2078 32 3225 32 2588
Fragments arranged by size:
3225 2588 2078 76 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8095
XbaI T'CTAG_A
Cuts at: 5431 5431
Size: 8095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2886 2886
Size: 8095
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8095
XmaI C'CCGG_G
Cuts at: 1870 5420 1870
Size: 3550 4545
XmnI GAAnn'nnTTC
Cuts at: 3613 4458 6224 3613
Size: 845 1766 5484
Fragments arranged by size:
5484 1766 845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5903 276
Size: 53 83 186 936 4369 2468
Fragments arranged by size:
4369 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FseI FspAI
KasI NarI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI