Restriction map for
pFN21AE3369
(Circular) (Six-base) MAPSORT of: pFN21AE3369.seq Check: 6564 from: 1 to: 7972
pFN21AE3369 7972 bp
With 182 enzymes: SgfI *
November 11, 2016 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5782 278
Size: 53 83 186 936 4246 2468
Fragments arranged by size:
4246 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4779 5315 1937
Size: 2842 536 4594
Fragments arranged by size:
4594 2842 536
Acc65I G'GTAC_C
Cuts at: 2506 4740 5293 2506
Size: 2234 553 5185
Fragments arranged by size:
5185 2234 553
AclI AA'CG_TT
Cuts at: 1563 6099 6472 1563
Size: 4536 373 3063
Fragments arranged by size:
4536 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2211 2950 3336 3566 3959 4934 5024 5160
Size: 579 739 386 230 393 975 90 136
Cuts at: 5160 5229 6035 7419 7687 1632
Size: 69 806 1384 268 1917
Fragments arranged by size:
1917 1384 975 806 739 579 393 386
268 230 136 90 69
AfeI AGC'GCT
Cuts at: 5044 5044
Size: 7972
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7775
AflIII A'CryG_T
Cuts at: 1203 6871 7848 1203
Size: 5668 977 1327
Fragments arranged by size:
5668 1327 977
AgeI A'CCGG_T
Cuts at: 6803 6803
Size: 7972
AhdI GACnn_n'nnGTC
Cuts at: 6701 6701
Size: 7972
AleI CACnn'nnGTG
Cuts at: 1166 2493 3101 4842 1166
Size: 1327 608 1741 4296
Fragments arranged by size:
4296 1741 1327 608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2798 1184
Size: 1614 6358
AlwNI CAG_nnn'CTG
Cuts at: 5038 5715 7287 5038
Size: 677 1572 5723
Fragments arranged by size:
5723 1572 677
ApaI G_GGCC'C
Cuts at: 2562 2562
Size: 7972
ApaLI G'TGCA_C
Cuts at: 3307 6029 7185 3307
Size: 2722 1156 4094
Fragments arranged by size:
4094 2722 1156
ApoI r'AATT_y
Cuts at: 1493 2128 3672 4896 5281 5507 5659 7768 1493
Size: 635 1544 1224 385 226 152 2109 1697
Fragments arranged by size:
2109 1697 1544 1224 635 385 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6526 160
Size: 872 5494 1606
Fragments arranged by size:
5494 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7972
AvaI C'yCGr_G
Cuts at: 1870 1943 2468 2837 3438 4413 5297 1870
Size: 73 525 369 601 975 884 4545
Fragments arranged by size:
4545 975 884 601 525 369 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3433 3466 3433
Size: 33 7939
BamHI G'GATC_C
Cuts at: 5302 5669 5302
Size: 367 7605
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2506 4682 4740 5293 6748
Size: 325 217 627 719 2176 58 553 1455
Cuts at: 6748 618
Size: 1842
Fragments arranged by size:
2176 1842 1455 719 627 553 325 217
58
BanII G_rGCy'C
Cuts at: 1391 2204 2562 5291 1391
Size: 813 358 2729 4072
Fragments arranged by size:
4072 2729 813 358
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2036 3171 5001 5214 5265 928
Size: 971 137 1135 1830 213 51 3635
Fragments arranged by size:
3635 1830 1135 971 213 137 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2858 2892 3724 3758 6163 6197 1903
Size: 34 921 34 832 34 2405 34 3678
Fragments arranged by size:
3678 2405 921 832 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5864 7074 5864
Size: 1210 6762
BclI T'GATC_A
Cuts at: 1548 3271 3618 1548
Size: 1723 347 5902
Fragments arranged by size:
5902 1723 347
BfrBI ATG'CAT
Cuts at: 4537 4964 4537
Size: 427 7545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2279 2885 6583
Size: 107 122 71 631 605 607 606 3698
Cuts at: 6583 136
Size: 1525
Fragments arranged by size:
3698 1525 631 607 606 605 122 107
71
BglII A'GATC_T
Cuts at: 4503 4665 7967 4503
Size: 162 3302 4508
Fragments arranged by size:
4508 3302 162
BlpI GC'TnA_GC
Cuts at: 2057 5389 2057
Size: 3332 4640
Bme1580I G_kGCm'C
Cuts at: 1790 2562 3311 6033 7189 1790
Size: 772 749 2722 1156 2573
Fragments arranged by size:
2722 2573 1156 772 749
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7972
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3297 5020 5703
Size: 416 53 83 399 24 1880 1723 683
Cuts at: 5703 6661 442
Size: 958 1753
Fragments arranged by size:
1880 1753 1723 958 683 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7972
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3451 3451
Size: 7972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2775 3700 3828 6632 1239
Size: 1536 925 128 2804 2579
Fragments arranged by size:
2804 2579 1536 925 128
Bpu10I CC'TnA_GC
Cuts at: 3156 4085 3156
Size: 929 7043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3222 3739 6097 6962 7260 3222
Size: 517 2358 865 298 3934
Fragments arranged by size:
3934 2358 865 517 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3482 4816 5192 6635 882
Size: 617 1983 1334 376 1443 2219
Fragments arranged by size:
2219 1983 1443 1334 617 376
BsaAI yAC'GTr
Cuts at: 493 1204 5763 493
Size: 711 4559 2702
Fragments arranged by size:
4559 2702 711
BsaBI GATnn'nnATC
Cuts at: 3617 7966 3617
Size: 4349 3623
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5779 6161 6796 275
Size: 53 83 186 936 4246 382 635 1451
Fragments arranged by size:
4246 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4743 6405 6803 7077 7224 7635 4743
Size: 1662 398 274 147 411 5080
Fragments arranged by size:
5080 1662 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4035 4065 1178
Size: 30 165 30 2632 30 5085
Fragments arranged by size:
5085 2632 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3500 5108 5111 1186
Size: 2314 1608 3 4047
Fragments arranged by size:
4047 2314 1608 3
BseYI C'CCAG_C
Cuts at: 3985 7175 7671 3985
Size: 3190 496 4286
Fragments arranged by size:
4286 3190 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2141 3979 1163
Size: 573 405 1838 5156
Fragments arranged by size:
5156 1838 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5422 6183 7211 1540
Size: 275 186 3421 761 1028 2301
Fragments arranged by size:
3421 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2204 3311 6033 6118 7189 2204
Size: 1107 2722 85 1071 2987
Fragments arranged by size:
2987 2722 1107 1071 85
BsmI GAATG_Cn'
Cuts at: 4650 5483 5576 4650
Size: 833 93 7046
Fragments arranged by size:
7046 833 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5150 5150
Size: 7972
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 2562 3311 5291 6033 6118 7189
Size: 399 414 358 749 1980 742 85 1071
Cuts at: 7189 1391
Size: 2174
Fragments arranged by size:
2174 1980 1071 749 742 414 399 358
85
BspEI T'CCGG_A
Cuts at: 7635 7635
Size: 7972
BspHI T'CATG_A
Cuts at: 3867 5861 7591 3867
Size: 1994 1730 4248
Fragments arranged by size:
4248 1994 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2518 2997 5327 844
Size: 1674 479 2330 3489
Fragments arranged by size:
3489 2330 1674 479
BsrBI CCG'CTC
Cuts at: 1394 1823 5859 1394
Size: 429 4036 3507
Fragments arranged by size:
4036 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6467 6641 65
Size: 1183 5219 174 1396
Fragments arranged by size:
5219 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3852 6616 6803 1700
Size: 110 2042 2764 187 2869
Fragments arranged by size:
2869 2764 2042 187 110
BsrGI T'GTAC_A
Cuts at: 96 2295 2820 96
Size: 2199 525 5248
Fragments arranged by size:
5248 2199 525
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7972
BssSI C'ACGA_G
Cuts at: 6032 7044 6032
Size: 1012 6960
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7972
BstBI TT'CG_AA
Cuts at: 4544 6776 4544
Size: 2232 5740
BstEII G'GTnAC_C
Cuts at: 1184 2701 1184
Size: 1517 6455
BstXI CCAn_nnnn'nTGG
Cuts at: 2815 3103 2815
Size: 288 7684
BstYI r'GATC_y
Cuts at: 1972 2647 3429 3905 4503 4665 5302 5669 6054
Size: 675 782 476 598 162 637 367 385
Cuts at: 6054 6071 7523 7609 7621 7967 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 782 675 637 598 476 385
367 346 162 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5091 5091
Size: 7972
BtgI C'CryG_G
Cuts at: 513 1065 2004 2844 3277 3453 3897 513
Size: 552 939 840 433 176 444 4588
Fragments arranged by size:
4588 939 840 552 444 433 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3478 3517 487
Size: 683 56 2252 39 4942
Fragments arranged by size:
4942 2252 683 56 39
BtsI GCAGTG_nn'
Cuts at: 2275 3885 5488 6282 6302 2275
Size: 1610 1603 794 20 3945
Fragments arranged by size:
3945 1610 1603 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7972
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7937
DraI TTT'AAA
Cuts at: 2628 5276 2628
Size: 2648 5324
DraIII CAC_nnn'GTG
Cuts at: 4631 4631
Size: 7972
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4334 6979 809
Size: 507 3018 2645 1802
Fragments arranged by size:
3018 2645 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3172 3399 3900
Size: 54 1425 50 275 18 1342 227 501
Cuts at: 3900 4521 4749 5419 6308 7937 8
Size: 621 228 670 889 1629 43
Fragments arranged by size:
1629 1425 1342 889 670 621 501 275
228 227 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5419 1537
Size: 275 3607 4090
Fragments arranged by size:
4090 3607 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 2322 3206 4814 4910 5136 5248 5902
Size: 837 132 884 1608 96 226 112 654
Cuts at: 5902 1353
Size: 3423
Fragments arranged by size:
3423 1608 884 837 654 226 132 112
96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6559 6933 7079 387
Size: 1064 138 4970 374 146 1280
Fragments arranged by size:
4970 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2202 2202
Size: 7972
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2211 2775 2950 3336 3566 3700 3828
Size: 393 579 564 175 386 230 134 128
Cuts at: 3828 3959 4934 5024 5160 5229 6035 6632 7419
Size: 131 975 90 136 69 806 597 787
Cuts at: 7419 7687 1239
Size: 268 1524
Fragments arranged by size:
1524 975 806 787 597 579 564 393
386 268 230 175 136 134 131 128
90 69
EcoO109I rG'GnC_Cy
Cuts at: 2544 2558 2559 4007 5101 2544
Size: 14 1 1448 1094 5415
Fragments arranged by size:
5415 1448 1094 14 1
EcoRI G'AATT_C
Cuts at: 2128 5281 5659 2128
Size: 3153 378 4441
Fragments arranged by size:
4441 3153 378
EcoRV GAT'ATC
Cuts at: 3045 4123 3045
Size: 1078 6894
FspI TGC'GCA
Cuts at: 6478 6478
Size: 7972
HaeII r_GCGC'y
Cuts at: 1678 5046 7119 1678
Size: 3368 2073 2531
Fragments arranged by size:
3368 2531 2073
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 3064 3096 3934
Size: 32 135 32 430 32 1030 32 838
Cuts at: 3934 3966 4035 4067 4346 4378 4625 4657 4909
Size: 32 69 32 279 32 247 32 252
Cuts at: 4909 4941 6613 6645 6687 6719 1373
Size: 32 1672 32 42 32 2626
Fragments arranged by size:
2626 1672 1030 838 430 279 252 247
135 69 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2734 2986 3234 4054 5316 5562
Size: 988 281 796 252 248 820 1262 246
Cuts at: 5562 669
Size: 3079
Fragments arranged by size:
3079 1262 988 820 796 281 252 248
246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7972
HpaI GTT'AAC
Cuts at: 3234 5562 3234
Size: 2328 5644
KpnI G_GTAC'C
Cuts at: 2510 4744 5297 2510
Size: 2234 553 5185
Fragments arranged by size:
5185 2234 553
MfeI C'AATT_G
Cuts at: 5571 5571
Size: 7972
MluI A'CGCG_T
Cuts at: 7848 7848
Size: 7972
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2955 4293 7086 7270 1305
Size: 1650 1338 2793 184 2007
Fragments arranged by size:
2793 2007 1650 1338 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3174 3401 4523 4751 10
Size: 54 1425 343 1342 227 1122 228 3231
Fragments arranged by size:
3231 1425 1342 1122 343 228 227 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 2493 2780 3101 3497 4202 4703
Size: 648 1197 130 287 321 396 705 501
Cuts at: 4703 4842 4946 5216 5930 6289 6448 518
Size: 139 104 270 714 359 159 2042
Fragments arranged by size:
2042 1197 714 705 648 501 396 359
321 287 270 159 139 130 104
MspA1I CmG'CkG
Cuts at: 1606 2125 2238 3314 3742 5388 6065 7213 7458
Size: 519 113 1076 428 1646 677 1148 245
Cuts at: 7458 1606
Size: 2120
Fragments arranged by size:
2120 1646 1148 1076 677 519 428 245
113
NcoI C'CATG_G
Cuts at: 513 1065 2004 3277 513
Size: 552 939 1273 5208
Fragments arranged by size:
5208 1273 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7972
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7972
NotI GC'GGCC_GC
Cuts at: 5419 5419
Size: 7972
NsiI A_TGCA'T
Cuts at: 4539 4966 4539
Size: 427 7545
NspI r_CATG'y
Cuts at: 1132 1229 4945 5330 6875 1132
Size: 97 3716 385 1545 2229
Fragments arranged by size:
3716 2229 1545 385 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7972
PciI A'CATG_T
Cuts at: 6871 6871
Size: 7972
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3776 1108
Size: 2668 5304
PfoI T'CCnGG_A
Cuts at: 3386 3386
Size: 7972
PmeI GTTT'AAAC
Cuts at: 5276 5276
Size: 7972
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6041 6073 7554 7586 6041
Size: 32 1481 32 6427
Fragments arranged by size:
6427 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2544 5101 2544
Size: 2557 5415
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7972
PsiI TTA'TAA
Cuts at: 5542 5542
Size: 7972
PspOMI G'GGCC_C
Cuts at: 2558 2558
Size: 7972
PstI C_TGCA'G
Cuts at: 830 3595 5324 830
Size: 2765 1729 3478
Fragments arranged by size:
3478 2765 1729
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7972
PvuII CAG'CTG
Cuts at: 2125 3314 3742 2125
Size: 1189 428 6355
Fragments arranged by size:
6355 1189 428
SacI G_AGCT'C
Cuts at: 2204 2204
Size: 7972
SalI G'TCGA_C
Cuts at: 1936 5314 1936
Size: 3378 4594
SapI GCTCTTCn'nnn_
Cuts at: 3206 3206
Size: 7972
SbfI CC_TGCA'GG
Cuts at: 5324 5324
Size: 7972
ScaI AGT'ACT
Cuts at: 6220 7932 6220
Size: 1712 6260
SexAI A'CCwGG_T
Cuts at: 5250 5250
Size: 7972
SfcI C'TryA_G
Cuts at: 826 1045 2553 3591 5320 6455 7136 7327 826
Size: 219 1508 1038 1729 1135 681 191 1471
Fragments arranged by size:
1729 1508 1471 1135 1038 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7972
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7972
SmaI CCC'GGG
Cuts at: 1872 5299 1872
Size: 3427 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3237 3754 6076 6977 7239 820
Size: 197 926 1294 517 2322 901 262 1553
Fragments arranged by size:
2322 1553 1294 926 901 517 262 197
SnaBI TAC'GTA
Cuts at: 493 5763 493
Size: 5270 2702
SpeI A'CTAG_T
Cuts at: 152 3781 152
Size: 3629 4343
SphI G_CATG'C
Cuts at: 1132 4945 5330 1132
Size: 3813 385 3774
Fragments arranged by size:
3813 3774 385
SspI AAT'ATT
Cuts at: 5 52 4265 5896 5
Size: 47 4213 1631 2081
Fragments arranged by size:
4213 2081 1631 47
StuI AGG'CCT
Cuts at: 1517 4371 1517
Size: 2854 5118
StyI C'CwwG_G
Cuts at: 513 1065 2004 3277 3523 4675 5411 513
Size: 552 939 1273 246 1152 736 3074
Fragments arranged by size:
3074 1273 1152 939 736 552 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4110 5999 6016 6169 6354 6799 325
Size: 1255 2530 1889 17 153 185 445 1498
Fragments arranged by size:
2530 1889 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2295 2820 6218
Size: 274 80 33 51 1443 318 525 3398
Cuts at: 6218 7930 96
Size: 1712 138
Fragments arranged by size:
3398 1712 1443 525 318 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2449 2481 3285 3313 3317 3345 3618 3650 4215
Size: 32 804 28 4 28 273 32 565
Cuts at: 4215 4247 4482 4514 7932 7964 2449
Size: 32 235 32 3418 32 2457
Fragments arranged by size:
3418 2457 804 565 273 235 32 32
32 32 32 28 28 4
Tth111I GACn'n_nGTC
Cuts at: 1316 3263 5084 1316
Size: 1947 1821 4204
Fragments arranged by size:
4204 1947 1821
XbaI T'CTAG_A
Cuts at: 2650 5308 2650
Size: 2658 5314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3259 3284 3259
Size: 25 7947
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7972
XmaI C'CCGG_G
Cuts at: 1870 5297 1870
Size: 3427 4545
XmnI GAAnn'nnTTC
Cuts at: 2942 3111 4896 6101 2942
Size: 169 1785 1205 4813
Fragments arranged by size:
4813 1785 1205 169
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5780 276
Size: 53 83 186 936 4246 2468
Fragments arranged by size:
4246 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BbvCI BsiWI BstZ17I
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI