Restriction map for
pFN21AE3368
(Circular) (Six-base) MAPSORT of: pFN21AE3368.seq Check: 6864 from: 1 to: 7969
pFN21AE3368 7969 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2784 3576 2784
Size: 792 7177
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5779 278
Size: 53 83 186 936 4243 2468
Fragments arranged by size:
4243 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5312 1937
Size: 3375 4594
Acc65I G'GTAC_C
Cuts at: 2567 5290 2567
Size: 2723 5246
AclI AA'CG_TT
Cuts at: 1563 6096 6469 1563
Size: 4533 373 3063
Fragments arranged by size:
4533 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4586 4793 4906 4925 5163 5265 6032 7416
Size: 2954 207 113 19 238 102 767 1384
Cuts at: 7416 7684 1632
Size: 268 1917
Fragments arranged by size:
2954 1917 1384 767 268 238 207 113
102 19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7772
AflIII A'CryG_T
Cuts at: 1203 6868 7845 1203
Size: 5665 977 1327
Fragments arranged by size:
5665 1327 977
AgeI A'CCGG_T
Cuts at: 6800 6800
Size: 7969
AhdI GACnn_n'nnGTC
Cuts at: 6698 6698
Size: 7969
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7969
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7969
AlwNI CAG_nnn'CTG
Cuts at: 2059 2323 3043 5712 7284 2059
Size: 264 720 2669 1572 2744
Fragments arranged by size:
2744 2669 1572 720 264
ApaI G_GGCC'C
Cuts at: 2585 2676 3384 3647 2585
Size: 91 708 263 6907
Fragments arranged by size:
6907 708 263 91
ApaLI G'TGCA_C
Cuts at: 6026 7182 6026
Size: 1156 6813
ApoI r'AATT_y
Cuts at: 1493 5005 5120 5278 5504 5656 7765 1493
Size: 3512 115 158 226 152 2109 1697
Fragments arranged by size:
3512 2109 1697 226 158 152 115
AseI AT'TA_AT
Cuts at: 160 1032 6523 160
Size: 872 5491 1606
Fragments arranged by size:
5491 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7969
AvaI C'yCGr_G
Cuts at: 1870 1943 2434 3656 4649 5294 1870
Size: 73 491 1222 993 645 4545
Fragments arranged by size:
4545 1222 993 645 491 73
BamHI G'GATC_C
Cuts at: 5299 5666 5299
Size: 367 7602
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2279 2567 2711 2782 2900
Size: 325 217 627 492 288 144 71 118
Cuts at: 2900 3166 3400 3497 3540 4034 4605 4747 5290
Size: 266 234 97 43 494 571 142 543
Cuts at: 5290 6745 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 571 543 494 492 325
288 266 234 217 144 142 118 97
71 43
BanII G_rGCy'C
Cuts at: 1391 2365 2490 2585 2676 2937 3002 3276 3384
Size: 974 125 95 91 261 65 274 108
Cuts at: 3384 3647 3657 4518 4829 5210 5288 1391
Size: 263 10 861 311 381 78 4072
Fragments arranged by size:
4072 974 861 381 311 274 263 261
125 108 95 91 78 65 10
BbeI G_GCGC'C
Cuts at: 2904 3170 3501 2904
Size: 266 331 7372
Fragments arranged by size:
7372 331 266
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 4100 4282 4574 928
Size: 971 192 2009 182 292 4323
Fragments arranged by size:
4323 2009 971 292 192 182
BbvCI CC'TCA_GC
Cuts at: 3353 3353
Size: 7969
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2051 2085 2371 2405 2525 2559 3550
Size: 34 114 34 286 34 120 34 991
Cuts at: 3550 3584 6160 6194 1903
Size: 34 2576 34 3678
Fragments arranged by size:
3678 2576 991 286 120 114 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5861 7071 5861
Size: 1210 6759
BclI T'GATC_A
Cuts at: 1548 4779 1548
Size: 3231 4738
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3128 6580 136
Size: 107 122 71 631 605 1456 3452 1525
Fragments arranged by size:
3452 1525 1456 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4402 7964 4402
Size: 3562 4407
BlpI GC'TnA_GC
Cuts at: 2561 3921 4823 5386 2561
Size: 1360 902 563 5144
Fragments arranged by size:
5144 1360 902 563
Bme1580I G_kGCm'C
Cuts at: 1790 2282 2585 2676 2737 3213 3384 3405 3545
Size: 492 303 91 61 476 171 21 140
Cuts at: 3545 3647 6030 7186 1790
Size: 102 2383 1156 2573
Fragments arranged by size:
2573 2383 1156 492 476 303 171 140
102 91 61 21
BmgBI CAC'GTC
Cuts at: 1318 3514 4141 1318
Size: 2196 627 5146
Fragments arranged by size:
5146 2196 627
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2495 3200 4455
Size: 416 53 83 399 24 1078 705 1255
Cuts at: 4455 4795 5700 6658 442
Size: 340 905 958 1753
Fragments arranged by size:
1753 1255 1078 958 905 705 416 399
340 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7969
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4727 4727
Size: 7969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 2636 3182 3905 4924 6629 1239
Size: 1184 213 546 723 1019 1705 2579
Fragments arranged by size:
2579 1705 1184 1019 723 546 213
Bpu10I CC'TnA_GC
Cuts at: 3353 4296 5103 3353
Size: 943 807 6219
Fragments arranged by size:
6219 943 807
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2254 2351 6094 6959 7257 2254
Size: 97 3743 865 298 2966
Fragments arranged by size:
3743 2966 865 298 97
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2843 6632 882
Size: 617 1344 3789 2219
Fragments arranged by size:
3789 2219 1344 617
BsaAI yAC'GTr
Cuts at: 493 1204 2638 2941 3184 3832 5266 5760 493
Size: 711 1434 303 243 648 1434 494 2702
Fragments arranged by size:
2702 1434 1434 711 648 494 303 243
BsaBI GATnn'nnATC
Cuts at: 7963 7963
Size: 7969
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2840 2901 3167 3459
Size: 53 83 186 936 1307 61 266 292
Cuts at: 3459 3498 5776 6158 6793 275
Size: 39 2278 382 635 1451
Fragments arranged by size:
2278 1451 1307 936 635 382 292 266
186 83 61 53 39
BsaWI w'CCGG_w
Cuts at: 3534 3870 4122 4278 4950 6402 6800 7074 7221
Size: 336 252 156 672 1452 398 274 147
Cuts at: 7221 7632 3534
Size: 411 3871
Fragments arranged by size:
3871 1452 672 411 398 336 274 252
156 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7744
Fragments arranged by size:
7744 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2633 3482 4649 1186
Size: 1447 849 1167 4506
Fragments arranged by size:
4506 1447 1167 849
BseYI C'CCAG_C
Cuts at: 2516 2995 3212 3484 4517 4767 7172 7668 2516
Size: 479 217 272 1033 250 2405 496 2817
Fragments arranged by size:
2817 2405 1033 496 479 272 250 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2831 4426 1163
Size: 573 1095 1595 4706
Fragments arranged by size:
4706 1595 1095 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2633 2771 3315 5419 6180 7208
Size: 275 186 632 138 544 2104 761 1028
Cuts at: 7208 1540
Size: 2301
Fragments arranged by size:
2301 2104 1028 761 632 544 275 186
138
BsiHKAI G_wGCw'C
Cuts at: 2365 4302 4659 5109 5210 6030 6115 7186 2365
Size: 1937 357 450 101 820 85 1071 3148
Fragments arranged by size:
3148 1937 1071 820 450 357 101 85
BsiWI C'GTAC_G
Cuts at: 2634 2634
Size: 7969
BsmI GAATG_Cn'
Cuts at: 5480 5573 5480
Size: 93 7876
BsmBI CGTCTCn'nnnn_
Cuts at: 3753 3753
Size: 7969
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2282 2365 2490 2585 2676 2737 2937
Size: 399 492 83 125 95 91 61 200
Cuts at: 2937 3002 3213 3276 3384 3405 3545 3647 3657
Size: 65 211 63 108 21 140 102 10
Cuts at: 3657 4302 4518 4659 4829 5109 5210 5288 6030
Size: 645 216 141 170 280 101 78 742
Cuts at: 6030 6115 7186 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 645 492 399 280 216
211 200 170 141 140 125 108 102
101 95 91 85 83 78 65 63
61 21 10
BspEI T'CCGG_A
Cuts at: 4278 4950 7632 4278
Size: 672 2682 4615
Fragments arranged by size:
4615 2682 672
BspHI T'CATG_A
Cuts at: 5858 7588 5858
Size: 1730 6239
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2784 3576 5324 844
Size: 1940 792 1748 3489
Fragments arranged by size:
3489 1940 1748 792
BsrBI CCG'CTC
Cuts at: 1394 1823 2565 5856 1394
Size: 429 742 3291 3507
Fragments arranged by size:
3507 3291 742 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6464 6638 65
Size: 1183 5216 174 1396
Fragments arranged by size:
5216 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2049 2304 2531 2903 2966 3115 6613
Size: 110 239 255 227 372 63 149 3498
Cuts at: 6613 6800 1700
Size: 187 2869
Fragments arranged by size:
3498 2869 372 255 239 227 187 149
110 63
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7969
BssHII G'CGCG_C
Cuts at: 1925 2754 3175 3177 1925
Size: 829 421 2 6717
Fragments arranged by size:
6717 829 421 2
BssSI C'ACGA_G
Cuts at: 6029 7041 6029
Size: 1012 6957
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2059 2314 2452 3043 3325 3571 794
Size: 1265 255 138 591 282 246 5192
Fragments arranged by size:
5192 1265 591 282 255 246 138
BstBI TT'CG_AA
Cuts at: 2523 6773 2523
Size: 4250 3719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7969
BstXI CCAn_nnnn'nTGG
Cuts at: 2158 2614 2158
Size: 456 7513
BstYI r'GATC_y
Cuts at: 1972 3942 4402 5299 5666 6051 6068 7520 7606
Size: 1970 460 897 367 385 17 1452 86
Cuts at: 7606 7618 7964 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1970 1452 897 460 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4100 4830 4100
Size: 730 7239
BtgI C'CryG_G
Cuts at: 513 1065 2607 2627 2668 2973 3245 4992 513
Size: 552 1542 20 41 305 272 1747 3490
Fragments arranged by size:
3490 1747 1542 552 305 272 41 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4303 4627 487
Size: 683 56 3077 324 3829
Fragments arranged by size:
3829 3077 683 324 56
BtsI GCAGTG_nn'
Cuts at: 3116 5485 6279 6299 3116
Size: 2369 794 20 4786
Fragments arranged by size:
4786 2369 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7969
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7934
DraI TTT'AAA
Cuts at: 5273 5273
Size: 7969
DraIII CAC_nnn'GTG
Cuts at: 3334 3334
Size: 7969
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6976 809
Size: 507 5660 1802
Fragments arranged by size:
5660 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2356 2630 2677
Size: 54 1425 50 275 18 526 274 47
Cuts at: 2677 2768 3113 3312 3897 4164 4335 5416 6305
Size: 91 345 199 585 267 171 1081 889
Cuts at: 6305 7934 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1081 889 585 526 345 275
274 267 199 171 91 54 50 47
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2630 2768 3312 5416 1537
Size: 275 818 138 544 2104 4090
Fragments arranged by size:
4090 2104 818 544 275 138
EarI CTCTTCn'nnn_
Cuts at: 1353 3332 3759 3764 4718 5899 1353
Size: 1979 427 5 954 1181 3423
Fragments arranged by size:
3423 1979 1181 954 427 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3246 3540 3717 4900 6556 6930
Size: 1064 138 1657 294 177 1183 1656 374
Cuts at: 6930 7076 387
Size: 146 1280
Fragments arranged by size:
1657 1656 1280 1183 1064 374 294 177
146 138
EcoICRI GAG'CTC
Cuts at: 2363 5208 2363
Size: 2845 5124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 2636 3182 3905 4586 4793 4906
Size: 393 791 213 546 723 681 207 113
Cuts at: 4906 4924 4925 5163 5265 6032 6629 7416 7684
Size: 18 1 238 102 767 597 787 268
Cuts at: 7684 1239
Size: 1524
Fragments arranged by size:
1524 791 787 767 723 681 597 546
393 268 238 213 207 113 102 18
1
EcoNI CCTnn'n_nnAGG
Cuts at: 4166 5131 4166
Size: 965 7004
EcoO109I rG'GnC_Cy
Cuts at: 2581 3380 3381 3644 4840 2581
Size: 799 1 263 1196 5710
Fragments arranged by size:
5710 1196 799 263 1
EcoRI G'AATT_C
Cuts at: 5120 5278 5656 5120
Size: 158 378 7433
Fragments arranged by size:
7433 378 158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4070 4378 4070
Size: 308 7661
FspI TGC'GCA
Cuts at: 3578 3616 6475 3578
Size: 38 2859 5072
Fragments arranged by size:
5072 2859 38
HaeII r_GCGC'y
Cuts at: 1678 2598 2615 2904 3170 3485 3501 3687 4929
Size: 920 17 289 266 315 16 186 1242
Cuts at: 4929 7116 1678
Size: 2187 2531
Fragments arranged by size:
2531 2187 1242 920 315 289 266 186
17 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4523 4555 6610 6642 6684
Size: 32 135 32 2951 32 2055 32 42
Cuts at: 6684 6716 1373
Size: 32 2626
Fragments arranged by size:
2951 2626 2055 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3868 5313 5559 669
Size: 988 281 1930 1445 246 3079
Fragments arranged by size:
3079 1930 1445 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2587 5190 748
Size: 1839 2603 3527
Fragments arranged by size:
3527 2603 1839
HpaI GTT'AAC
Cuts at: 3868 5559 3868
Size: 1691 6278
KasI G'GCGC_C
Cuts at: 2900 3166 3497 2900
Size: 266 331 7372
Fragments arranged by size:
7372 331 266
KpnI G_GTAC'C
Cuts at: 2571 5294 2571
Size: 2723 5246
MfeI C'AATT_G
Cuts at: 5568 5568
Size: 7969
MluI A'CGCG_T
Cuts at: 7845 7845
Size: 7969
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3567 4681 7083 7267 1305
Size: 2262 1114 2402 184 2007
Fragments arranged by size:
2402 2262 2007 1114 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3899 4337 10
Size: 54 1425 343 2067 438 3642
Fragments arranged by size:
3642 2067 1425 438 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5927 6286 6445 518
Size: 648 4761 359 159 2042
Fragments arranged by size:
4761 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2291 2560 2629 2746 2975 2986 3247 3508
Size: 685 269 69 117 229 11 261 261
Cuts at: 3508 3883 4771 5385 6062 7210 7455 1606
Size: 375 888 614 677 1148 245 2120
Fragments arranged by size:
2120 1148 888 685 677 614 375 269
261 261 245 229 117 69 11
NaeI GCC'GGC
Cuts at: 2051 2905 3117 2051
Size: 854 212 6903
Fragments arranged by size:
6903 854 212
NarI GG'CG_CC
Cuts at: 2901 3167 3498 2901
Size: 266 331 7372
Fragments arranged by size:
7372 331 266
NcoI C'CATG_G
Cuts at: 513 1065 4992 513
Size: 552 3927 3490
Fragments arranged by size:
3927 3490 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7969
NgoMIV G'CCGG_C
Cuts at: 2049 2903 3115 2049
Size: 854 212 6903
Fragments arranged by size:
6903 854 212
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7969
NotI GC'GGCC_GC
Cuts at: 2768 5416 2768
Size: 2648 5321
NspI r_CATG'y
Cuts at: 1132 1229 2993 3476 5327 6872 1132
Size: 97 1764 483 1851 1545 2229
Fragments arranged by size:
2229 1851 1764 1545 483 97
PasI CC'CwG_GG
Cuts at: 1348 2797 4024 1348
Size: 1449 1227 5293
Fragments arranged by size:
5293 1449 1227
PciI A'CATG_T
Cuts at: 6868 6868
Size: 7969
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 4357 4879 4993 5067 1108
Size: 1242 2007 522 114 74 4010
Fragments arranged by size:
4010 2007 1242 522 114 74
PmeI GTTT'AAAC
Cuts at: 5273 5273
Size: 7969
PmlI CAC'GTG
Cuts at: 3184 3832 3184
Size: 648 7321
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6038 6070 7551 7583 6038
Size: 32 1481 32 6424
Fragments arranged by size:
6424 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4840 4840
Size: 7969
PshAI GACnn'nnGTC
Cuts at: 1613 3305 1613
Size: 1692 6277
PsiI TTA'TAA
Cuts at: 5539 5539
Size: 7969
PspOMI G'GGCC_C
Cuts at: 2581 2672 3380 3643 2581
Size: 91 708 263 6907
Fragments arranged by size:
6907 708 263 91
PstI C_TGCA'G
Cuts at: 830 2290 2296 2319 2751 3150 3882 5321 830
Size: 1460 6 23 432 399 732 1439 3478
Fragments arranged by size:
3478 1460 1439 732 432 399 23 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7969
PvuII CAG'CTG
Cuts at: 2291 2560 2986 3883 4771 2291
Size: 269 426 897 888 5489
Fragments arranged by size:
5489 897 888 426 269
RsrII CG'GwC_CG
Cuts at: 3254 3254
Size: 7969
SacI G_AGCT'C
Cuts at: 2365 5210 2365
Size: 2845 5124
SacII CC_GC'GG
Cuts at: 2630 2976 3248 2630
Size: 346 272 7351
Fragments arranged by size:
7351 346 272
SalI G'TCGA_C
Cuts at: 1936 5311 1936
Size: 3375 4594
SapI GCTCTTCn'nnn_
Cuts at: 3332 3332
Size: 7969
SbfI CC_TGCA'GG
Cuts at: 5321 5321
Size: 7969
ScaI AGT'ACT
Cuts at: 6217 7929 6217
Size: 1712 6257
SfcI C'TryA_G
Cuts at: 826 1045 2286 2292 2315 2747 3146 3878 5317
Size: 219 1241 6 23 432 399 732 1439
Cuts at: 5317 6452 7133 7324 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1439 1241 1135 732 681 432 399
219 191 23 6
SfoI GGC'GCC
Cuts at: 2902 3168 3499 2902
Size: 266 331 7372
Fragments arranged by size:
7372 331 266
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7969
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7969
SmaI CCC'GGG
Cuts at: 1872 5296 1872
Size: 3424 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2233 2366 6073 6974 7236 820
Size: 197 926 290 133 3707 901 262 1553
Fragments arranged by size:
3707 1553 926 901 290 262 197 133
SnaBI TAC'GTA
Cuts at: 493 5760 493
Size: 5267 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7969
SphI G_CATG'C
Cuts at: 1132 3476 5327 1132
Size: 2344 1851 3774
Fragments arranged by size:
3774 2344 1851
SspI AAT'ATT
Cuts at: 5 52 4475 5893 5
Size: 47 4423 1418 2081
Fragments arranged by size:
4423 2081 1418 47
StuI AGG'CCT
Cuts at: 1517 5066 1517
Size: 3549 4420
StyI C'CwwG_G
Cuts at: 513 1065 2700 3462 3687 4992 5033 5061 5408
Size: 552 1635 762 225 1305 41 28 347
Cuts at: 5408 513
Size: 3074
Fragments arranged by size:
3074 1635 1305 762 552 347 225 41
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2709 2713 2964 3388 4174 5996 6013
Size: 1255 1129 4 251 424 786 1822 17
Cuts at: 6013 6166 6351 6796 325
Size: 153 185 445 1498
Fragments arranged by size:
1822 1498 1255 1129 786 445 424 251
185 153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2406 4227 6215
Size: 274 80 33 51 1443 429 1821 1988
Cuts at: 6215 7927 96
Size: 1712 138
Fragments arranged by size:
1988 1821 1712 1443 429 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3596 3628 7929 7961 3596
Size: 32 4301 32 3604
Fragments arranged by size:
4301 3604 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7969
XbaI T'CTAG_A
Cuts at: 5305 5305
Size: 7969
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2351 2351
Size: 7969
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7969
XmaI C'CCGG_G
Cuts at: 1870 5294 1870
Size: 3424 4545
XmnI GAAnn'nnTTC
Cuts at: 6098 6098
Size: 7969
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5777 276
Size: 53 83 186 936 4243 2468
Fragments arranged by size:
4243 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PfoI PspXI PsrI
SanDI SexAI SfiI SrfI SwaI