Restriction map for
pFN21AE3363
(Circular) (Six-base) MAPSORT of: pFN21AE3363.seq Check: 3446 from: 1 to: 7915
pFN21AE3363 7915 bp
With 182 enzymes: SgfI *
November 16, 2016 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3182 4001 3182
Size: 819 7096
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5725 278
Size: 53 83 186 936 4189 2468
Fragments arranged by size:
4189 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3111 3168 5258 1937
Size: 1174 57 2090 4594
Fragments arranged by size:
4594 2090 1174 57
Acc65I G'GTAC_C
Cuts at: 2084 3438 5236 2084
Size: 1354 1798 4763
Fragments arranged by size:
4763 1798 1354
AclI AA'CG_TT
Cuts at: 1563 6042 6415 1563
Size: 4479 373 3063
Fragments arranged by size:
4479 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2417 3269 5149 5978 7362 7630 1632
Size: 785 852 1880 829 1384 268 1917
Fragments arranged by size:
1917 1880 1384 852 829 785 268
AfeI AGC'GCT
Cuts at: 4190 5129 4190
Size: 939 6976
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7718
AflIII A'CryG_T
Cuts at: 1203 2188 6814 7791 1203
Size: 985 4626 977 1327
Fragments arranged by size:
4626 1327 985 977
AgeI A'CCGG_T
Cuts at: 6746 6746
Size: 7915
AhdI GACnn_n'nnGTC
Cuts at: 3212 6644 3212
Size: 3432 4483
AleI CACnn'nnGTG
Cuts at: 1166 3538 1166
Size: 2372 5543
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7915
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2717 2749 3359 3391 2717
Size: 32 610 32 7241
Fragments arranged by size:
7241 610 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2387 4525 4663 5658 7230 2387
Size: 2138 138 995 1572 3072
Fragments arranged by size:
3072 2138 1572 995 138
ApaI G_GGCC'C
Cuts at: 2183 2215 2930 4082 5140 2183
Size: 32 715 1152 1058 4958
Fragments arranged by size:
4958 1152 1058 715 32
ApaLI G'TGCA_C
Cuts at: 5972 7128 5972
Size: 1156 6759
ApoI r'AATT_y
Cuts at: 1493 5224 5450 5602 7711 1493
Size: 3731 226 152 2109 1697
Fragments arranged by size:
3731 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6469 160
Size: 872 5437 1606
Fragments arranged by size:
5437 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7915
AvaI C'yCGr_G
Cuts at: 1870 1943 2313 2764 3678 3687 4220 4928 5090
Size: 73 370 451 914 9 533 708 162
Cuts at: 5090 5240 1870
Size: 150 4545
Fragments arranged by size:
4545 914 708 533 451 370 162 150
73 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2177 2210 3430 3463 2177
Size: 33 1220 33 6629
Fragments arranged by size:
6629 1220 33 33
BamHI G'GATC_C
Cuts at: 2021 5245 5612 2021
Size: 3224 367 4324
Fragments arranged by size:
4324 3224 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2142 2294 2466 2692
Size: 325 217 627 297 58 152 172 226
Cuts at: 2692 3438 3568 4133 4906 5236 6691 618
Size: 746 130 565 773 330 1455 1842
Fragments arranged by size:
1842 1455 773 746 627 565 330 325
297 226 217 172 152 130 58
BanII G_rGCy'C
Cuts at: 1391 2183 2215 2930 3060 4082 4325 4614 5140
Size: 792 32 715 130 1022 243 289 526
Cuts at: 5140 5234 1391
Size: 94 4072
Fragments arranged by size:
4072 1022 792 715 526 289 243 130
94 32
BbeI G_GCGC'C
Cuts at: 2146 2146
Size: 7915
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2095 2979 3376 928
Size: 971 196 884 397 5467
Fragments arranged by size:
5467 971 884 397 196
BbvCI CC'TCA_GC
Cuts at: 4282 4709 4856 5085 5141 4282
Size: 427 147 229 56 7056
Fragments arranged by size:
7056 427 229 147 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6106 6140 1903
Size: 34 4169 34 3678
Fragments arranged by size:
4169 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5807 7017 5807
Size: 1210 6705
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7915
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3191 3244 4142
Size: 107 122 71 631 605 1519 53 898
Cuts at: 4142 6526 136
Size: 2384 1525
Fragments arranged by size:
2384 1525 1519 898 631 605 122 107
71 53
BglII A'GATC_T
Cuts at: 7910 7910
Size: 7915
BlpI GC'TnA_GC
Cuts at: 2054 4608 5332 2054
Size: 2554 724 4637
Fragments arranged by size:
4637 2554 724
Bme1580I G_kGCm'C
Cuts at: 1790 2183 2215 2469 2930 3240 3573 3751 4082
Size: 393 32 254 461 310 333 178 331
Cuts at: 4082 4825 5140 5976 7132 1790
Size: 743 315 836 1156 2573
Fragments arranged by size:
2573 1156 836 743 461 393 333 331
315 310 254 178 32
BmgBI CAC'GTC
Cuts at: 1318 2704 3964 1318
Size: 1386 1260 5269
Fragments arranged by size:
5269 1386 1260
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5646 6604 442
Size: 416 53 83 399 24 4229 958 1753
Fragments arranged by size:
4229 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2349 3293 4961 6575 1239
Size: 1110 944 1668 1614 2579
Fragments arranged by size:
2579 1668 1614 1110 944
Bpu10I CC'TnA_GC
Cuts at: 2258 2580 4282 4449 4709 4856 5085 5141 2258
Size: 322 1702 167 260 147 229 56 5032
Fragments arranged by size:
5032 1702 322 260 229 167 147 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2674 3494 4637 6040 6905 7203 2674
Size: 820 1143 1403 865 298 3386
Fragments arranged by size:
3386 1403 1143 865 820 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2862 3849 4469 4641 6578 882
Size: 617 1363 987 620 172 1937 2219
Fragments arranged by size:
2219 1937 1363 987 620 617 172
BsaAI yAC'GTr
Cuts at: 493 1204 5706 493
Size: 711 4502 2702
Fragments arranged by size:
4502 2702 711
BsaBI GATnn'nnATC
Cuts at: 3356 3929 7909 3356
Size: 573 3980 3362
Fragments arranged by size:
3980 3362 573
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2143 5722 6104 6739
Size: 53 83 186 936 610 3579 382 635
Cuts at: 6739 275
Size: 1451
Fragments arranged by size:
3579 1451 936 635 610 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4533 6348 6746 7020 7167 7578 4533
Size: 1815 398 274 147 411 4870
Fragments arranged by size:
4870 1815 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2717 2747 4751 4781 5144
Size: 30 165 30 1314 30 2004 30 363
Cuts at: 5144 5174 1178
Size: 30 3919
Fragments arranged by size:
3919 2004 1314 363 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2738 3032 3290 4082 4244 4475 1186
Size: 1552 294 258 792 162 231 4626
Fragments arranged by size:
4626 1552 792 294 258 231 162
BseYI C'CCAG_C
Cuts at: 2077 2754 3059 3460 3528 3659 3913 4162 4408
Size: 677 305 401 68 131 254 249 246
Cuts at: 4408 5066 5133 7118 7614 2077
Size: 658 67 1985 496 2378
Fragments arranged by size:
2378 1985 677 658 496 401 305 254
249 246 131 68 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7342
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5365 6126 7154 1540
Size: 275 186 3364 761 1028 2301
Fragments arranged by size:
3364 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3078 4764 5976 6061 7132 3078
Size: 1686 1212 85 1071 3861
Fragments arranged by size:
3861 1686 1212 1071 85
BsiWI C'GTAC_G
Cuts at: 3803 3803
Size: 7915
BsmI GAATG_Cn'
Cuts at: 4250 5426 5519 4250
Size: 1176 93 6646
Fragments arranged by size:
6646 1176 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2183 2215 2469 2930 3060 3078 3240
Size: 399 393 32 254 461 130 18 162
Cuts at: 3240 3573 3751 4082 4325 4614 4764 4825 5140
Size: 333 178 331 243 289 150 61 315
Cuts at: 5140 5234 5976 6061 7132 1391
Size: 94 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 461 399 393 333 331
315 289 254 243 178 162 150 130
94 85 61 32 18
BspEI T'CCGG_A
Cuts at: 4533 7578 4533
Size: 3045 4870
BspHI T'CATG_A
Cuts at: 5804 7534 5804
Size: 1730 6185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2372 3038 3182 4001 5270 844
Size: 1528 666 144 819 1269 3489
Fragments arranged by size:
3489 1528 1269 819 666 144
BsrBI CCG'CTC
Cuts at: 1394 1823 2233 2551 5802 1394
Size: 429 410 318 3251 3507
Fragments arranged by size:
3507 3251 429 410 318
BsrDI GCAATG_nn'
Cuts at: 65 1248 2658 2737 2893 3714 3996 4152 6410
Size: 1183 1410 79 156 821 282 156 2258
Cuts at: 6410 6584 65
Size: 174 1396
Fragments arranged by size:
2258 1410 1396 1183 821 282 174 156
156 79
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 2277 3464 5154 5212 6559 6746
Size: 110 230 237 1187 1690 58 1347 187
Cuts at: 6746 1700
Size: 2869
Fragments arranged by size:
2869 1690 1347 1187 237 230 187 110
58
BsrGI T'GTAC_A
Cuts at: 96 4869 96
Size: 4773 3142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7915
BssSI C'ACGA_G
Cuts at: 3125 5975 6987 3125
Size: 2850 1012 4053
Fragments arranged by size:
4053 2850 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4011 794
Size: 3217 4698
BstBI TT'CG_AA
Cuts at: 6719 6719
Size: 7915
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7915
BstXI CCAn_nnnn'nTGG
Cuts at: 2462 2941 3913 4118 5185 2462
Size: 479 972 205 1067 5192
Fragments arranged by size:
5192 1067 972 479 205
BstYI r'GATC_y
Cuts at: 1972 2021 2421 2895 4290 4639 5245 5612 5997
Size: 49 400 474 1395 349 606 367 385
Cuts at: 5997 6014 7466 7552 7564 7910 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1395 606 474 400 385 367
349 346 86 49 17 12
Bsu36I CC'TnA_GG
Cuts at: 3972 4664 3972
Size: 692 7223
BtgI C'CryG_G
Cuts at: 513 1065 2004 2993 3100 3564 4332 4379 5023
Size: 552 939 989 107 464 768 47 644
Cuts at: 5023 5199 513
Size: 176 3229
Fragments arranged by size:
3229 989 939 768 644 552 464 176
107 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7176
Fragments arranged by size:
7176 683 56
BtsI GCAGTG_nn'
Cuts at: 2132 2350 2780 3545 3845 5431 6225 6245 2132
Size: 218 430 765 300 1586 794 20 3802
Fragments arranged by size:
3802 1586 794 765 430 300 218 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7915
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2827 2862 529
Size: 35 2263 35 5582
Fragments arranged by size:
5582 2263 35 35
DraI TTT'AAA
Cuts at: 5219 5219
Size: 7915
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6922 809
Size: 507 5606 1802
Fragments arranged by size:
5606 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2567 2623
Size: 54 1425 50 275 18 198 539 56
Cuts at: 2623 2879 2911 3138 3183 3228 4335 5362 6251
Size: 256 32 227 45 45 1107 1027 889
Cuts at: 6251 7880 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1107 1027 889 539 275 256
227 198 56 54 50 45 45 43
32 18
EagI C'GGCC_G
Cuts at: 1537 1812 5362 1537
Size: 275 3550 4090
Fragments arranged by size:
4090 3550 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4409 4487 5845 1353
Size: 3056 78 1358 3423
Fragments arranged by size:
3423 3056 1358 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4883 6502 6876 7022 387
Size: 1064 138 3294 1619 374 146 1280
Fragments arranged by size:
3294 1619 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2349 2417 3269 3293 4961 5149 5978
Size: 393 717 68 852 24 1668 188 829
Cuts at: 5978 6575 7362 7630 1239
Size: 597 787 268 1524
Fragments arranged by size:
1668 1524 852 829 787 717 597 393
268 188 68 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3308 4442 4859 3308
Size: 1134 417 6364
Fragments arranged by size:
6364 1134 417
EcoO109I rG'GnC_Cy
Cuts at: 2211 2212 2384 2502 2926 3396 3663 3917 4033
Size: 1 172 118 424 470 267 254 116
Cuts at: 4033 4078 4079 5137 2211
Size: 45 1 1058 4989
Fragments arranged by size:
4989 1058 470 424 267 254 172 118
116 45 1 1
EcoRI G'AATT_C
Cuts at: 5224 5602 5224
Size: 378 7537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2969 4390 2969
Size: 1421 6494
FspI TGC'GCA
Cuts at: 3404 6421 3404
Size: 3017 4898
HaeII r_GCGC'y
Cuts at: 1678 2146 2153 4192 5131 5184 7062 1678
Size: 468 7 2039 939 53 1878 2531
Fragments arranged by size:
2531 2039 1878 939 468 53 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3198 3230 4751 4783 5144
Size: 32 135 32 1626 32 1521 32 361
Cuts at: 5144 5176 6556 6588 6630 6662 1373
Size: 32 1380 32 42 32 2626
Fragments arranged by size:
2626 1626 1521 1380 361 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3217 5259 5505 669
Size: 988 281 1279 2042 246 3079
Fragments arranged by size:
3079 2042 1279 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2671 748
Size: 1923 5992
HpaI GTT'AAC
Cuts at: 5505 5505
Size: 7915
KasI G'GCGC_C
Cuts at: 2142 2142
Size: 7915
KpnI G_GTAC'C
Cuts at: 2088 3442 5240 2088
Size: 1354 1798 4763
Fragments arranged by size:
4763 1798 1354
MfeI C'AATT_G
Cuts at: 5514 5514
Size: 7915
MluI A'CGCG_T
Cuts at: 7791 7791
Size: 7915
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3747 4053 4524 4794 7029 7213 1305
Size: 2442 306 471 270 2235 184 2007
Fragments arranged by size:
2442 2235 2007 471 306 270 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2625 2913 3140 3230 10
Size: 54 1425 343 793 288 227 90 4695
Fragments arranged by size:
4695 1425 793 343 288 227 90 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2460 3224 3538 4157 5873 6232 6391
Size: 648 1294 764 314 619 1716 359 159
Cuts at: 6391 518
Size: 2042
Fragments arranged by size:
2042 1716 1294 764 648 619 359 314
159
MspA1I CmG'CkG
Cuts at: 1606 2074 2270 2573 2904 3008 3202 3271 3868
Size: 468 196 303 331 104 194 69 597
Cuts at: 3868 4011 4139 4334 4525 4912 5331 6008 7156
Size: 143 128 195 191 387 419 677 1148
Cuts at: 7156 7401 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 597 468 419 387 331
303 245 196 195 194 191 143 128
104 69
NaeI GCC'GGC
Cuts at: 3466 3466
Size: 7915
NarI GG'CG_CC
Cuts at: 2143 2143
Size: 7915
NcoI C'CATG_G
Cuts at: 513 1065 2004 3100 5023 513
Size: 552 939 1096 1923 3405
Fragments arranged by size:
3405 1923 1096 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7915
NgoMIV G'CCGG_C
Cuts at: 3464 3464
Size: 7915
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7915
NotI GC'GGCC_GC
Cuts at: 5362 5362
Size: 7915
NspI r_CATG'y
Cuts at: 1132 1229 2192 2980 5273 6818 1132
Size: 97 963 788 2293 1545 2229
Fragments arranged by size:
2293 2229 1545 963 788 97
PasI CC'CwG_GG
Cuts at: 1348 2245 3850 4750 4849 5185 1348
Size: 897 1605 900 99 336 4078
Fragments arranged by size:
4078 1605 900 897 336 99
PciI A'CATG_T
Cuts at: 2188 6814 2188
Size: 4626 3289
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2308 2704 3066 3130 3499 3964 4621 1108
Size: 1200 396 362 64 369 465 657 4402
Fragments arranged by size:
4402 1200 657 465 396 369 362 64
PfoI T'CCnGG_A
Cuts at: 2220 2940 3897 4938 2220
Size: 720 957 1041 5197
Fragments arranged by size:
5197 1041 957 720
PmeI GTTT'AAAC
Cuts at: 5219 5219
Size: 7915
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2717 2749 5984 6016 7497 7529 2717
Size: 32 3235 32 1481 32 3103
Fragments arranged by size:
3235 3103 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2384 2502 3396 3663 3917 2384
Size: 118 894 267 254 6382
Fragments arranged by size:
6382 894 267 254 118
PshAI GACnn'nnGTC
Cuts at: 1613 2438 2873 1613
Size: 825 435 6655
Fragments arranged by size:
6655 825 435
PsiI TTA'TAA
Cuts at: 5485 5485
Size: 7915
PspOMI G'GGCC_C
Cuts at: 2179 2211 2926 4078 5136 2179
Size: 32 715 1152 1058 4958
Fragments arranged by size:
4958 1152 1058 715 32
PstI C_TGCA'G
Cuts at: 830 2139 2286 2775 3840 4212 4395 4503 5267
Size: 1309 147 489 1065 372 183 108 764
Cuts at: 5267 830
Size: 3478
Fragments arranged by size:
3478 1309 1065 764 489 372 183 147
108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7915
PvuII CAG'CTG
Cuts at: 2270 2573 3008 3271 4011 4525 2270
Size: 303 435 263 740 514 5660
Fragments arranged by size:
5660 740 514 435 303 263
SacII CC_GC'GG
Cuts at: 4335 4335
Size: 7915
SalI G'TCGA_C
Cuts at: 1936 5257 1936
Size: 3321 4594
SanDI GG'GwC_CC
Cuts at: 2502 3663 3917 2502
Size: 1161 254 6500
Fragments arranged by size:
6500 1161 254
SapI GCTCTTCn'nnn_
Cuts at: 4487 4487
Size: 7915
SbfI CC_TGCA'GG
Cuts at: 5267 5267
Size: 7915
ScaI AGT'ACT
Cuts at: 4883 5049 6163 7875 4883
Size: 166 1114 1712 4923
Fragments arranged by size:
4923 1712 1114 166
SfcI C'TryA_G
Cuts at: 826 1045 2135 2282 2771 3327 3836 4208 4391
Size: 219 1090 147 489 556 509 372 183
Cuts at: 4391 4499 5263 6398 7079 7270 826
Size: 108 764 1135 681 191 1471
Fragments arranged by size:
1471 1135 1090 764 681 556 509 489
372 219 191 183 147 108
SfoI GGC'GCC
Cuts at: 2144 2144
Size: 7915
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7915
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7915
SmaI CCC'GGG
Cuts at: 1872 3680 5242 1872
Size: 1808 1562 4545
Fragments arranged by size:
4545 1808 1562
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2653 3509 3687 4652 6019 6920
Size: 197 926 710 856 178 965 1367 901
Cuts at: 6920 7182 820
Size: 262 1553
Fragments arranged by size:
1553 1367 965 926 901 856 710 262
197 178
SnaBI TAC'GTA
Cuts at: 493 5706 493
Size: 5213 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7915
SphI G_CATG'C
Cuts at: 1132 5273 1132
Size: 4141 3774
SspI AAT'ATT
Cuts at: 5 52 3145 5839 5
Size: 47 3093 2694 2081
Fragments arranged by size:
3093 2694 2081 47
StuI AGG'CCT
Cuts at: 1517 4901 1517
Size: 3384 4531
StyI C'CwwG_G
Cuts at: 513 1065 2004 3042 3100 5023 5148 5354 513
Size: 552 939 1038 58 1923 125 206 3074
Fragments arranged by size:
3074 1923 1038 939 552 206 125 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2947 3113 3634 4121 4754 4894 5942
Size: 1255 1367 166 521 487 633 140 1048
Cuts at: 5942 5959 6112 6297 6742 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1367 1255 1048 633 521 487 445
185 166 153 140 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2193 4869 4881
Size: 274 80 33 51 1443 216 2676 12
Cuts at: 4881 5047 6161 7873 96
Size: 166 1114 1712 138
Fragments arranged by size:
2676 1712 1443 1114 274 216 166 138
80 51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2922 2954 7875 7907 2922
Size: 32 4921 32 2930
Fragments arranged by size:
4921 2930 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7915
XbaI T'CTAG_A
Cuts at: 5251 5251
Size: 7915
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2618 2686 4405 4723 2618
Size: 68 1719 318 5810
Fragments arranged by size:
5810 1719 318 68
XhoI C'TCGA_G
Cuts at: 1943 3687 1943
Size: 1744 6171
XmaI C'CCGG_G
Cuts at: 1870 3678 5240 1870
Size: 1808 1562 4545
Fragments arranged by size:
4545 1808 1562
XmnI GAAnn'nnTTC
Cuts at: 6044 6044
Size: 7915
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5723 276
Size: 53 83 186 936 4189 2468
Fragments arranged by size:
4189 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BplI BsmBI BstZ17I DraIII EcoICRI
EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SexAI SfiI SrfI SwaI