(Circular) (Six-base) MAPSORT of: pFN21AE3363.seq Check: 3446 from: 1 to: 7915 pFN21AE3363 7915 bp With 182 enzymes: SgfI * November 16, 2016 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3182 4001 3182 Size: 819 7096 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5725 278 Size: 53 83 186 936 4189 2468 Fragments arranged by size: 4189 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3111 3168 5258 1937 Size: 1174 57 2090 4594 Fragments arranged by size: 4594 2090 1174 57 Acc65I G'GTAC_C Cuts at: 2084 3438 5236 2084 Size: 1354 1798 4763 Fragments arranged by size: 4763 1798 1354 AclI AA'CG_TT Cuts at: 1563 6042 6415 1563 Size: 4479 373 3063 Fragments arranged by size: 4479 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2417 3269 5149 5978 7362 7630 1632 Size: 785 852 1880 829 1384 268 1917 Fragments arranged by size: 1917 1880 1384 852 829 785 268 AfeI AGC'GCT Cuts at: 4190 5129 4190 Size: 939 6976 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7718 AflIII A'CryG_T Cuts at: 1203 2188 6814 7791 1203 Size: 985 4626 977 1327 Fragments arranged by size: 4626 1327 985 977 AgeI A'CCGG_T Cuts at: 6746 6746 Size: 7915 AhdI GACnn_n'nnGTC Cuts at: 3212 6644 3212 Size: 3432 4483 AleI CACnn'nnGTG Cuts at: 1166 3538 1166 Size: 2372 5543 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7915 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2717 2749 3359 3391 2717 Size: 32 610 32 7241 Fragments arranged by size: 7241 610 32 32 AlwNI CAG_nnn'CTG Cuts at: 2387 4525 4663 5658 7230 2387 Size: 2138 138 995 1572 3072 Fragments arranged by size: 3072 2138 1572 995 138 ApaI G_GGCC'C Cuts at: 2183 2215 2930 4082 5140 2183 Size: 32 715 1152 1058 4958 Fragments arranged by size: 4958 1152 1058 715 32 ApaLI G'TGCA_C Cuts at: 5972 7128 5972 Size: 1156 6759 ApoI r'AATT_y Cuts at: 1493 5224 5450 5602 7711 1493 Size: 3731 226 152 2109 1697 Fragments arranged by size: 3731 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6469 160 Size: 872 5437 1606 Fragments arranged by size: 5437 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7915 AvaI C'yCGr_G Cuts at: 1870 1943 2313 2764 3678 3687 4220 4928 5090 Size: 73 370 451 914 9 533 708 162 Cuts at: 5090 5240 1870 Size: 150 4545 Fragments arranged by size: 4545 914 708 533 451 370 162 150 73 9 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2177 2210 3430 3463 2177 Size: 33 1220 33 6629 Fragments arranged by size: 6629 1220 33 33 BamHI G'GATC_C Cuts at: 2021 5245 5612 2021 Size: 3224 367 4324 Fragments arranged by size: 4324 3224 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 2142 2294 2466 2692 Size: 325 217 627 297 58 152 172 226 Cuts at: 2692 3438 3568 4133 4906 5236 6691 618 Size: 746 130 565 773 330 1455 1842 Fragments arranged by size: 1842 1455 773 746 627 565 330 325 297 226 217 172 152 130 58 BanII G_rGCy'C Cuts at: 1391 2183 2215 2930 3060 4082 4325 4614 5140 Size: 792 32 715 130 1022 243 289 526 Cuts at: 5140 5234 1391 Size: 94 4072 Fragments arranged by size: 4072 1022 792 715 526 289 243 130 94 32 BbeI G_GCGC'C Cuts at: 2146 2146 Size: 7915 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2095 2979 3376 928 Size: 971 196 884 397 5467 Fragments arranged by size: 5467 971 884 397 196 BbvCI CC'TCA_GC Cuts at: 4282 4709 4856 5085 5141 4282 Size: 427 147 229 56 7056 Fragments arranged by size: 7056 427 229 147 56 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6106 6140 1903 Size: 34 4169 34 3678 Fragments arranged by size: 4169 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5807 7017 5807 Size: 1210 6705 BclI T'GATC_A Cuts at: 1548 1548 Size: 7915 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3191 3244 4142 Size: 107 122 71 631 605 1519 53 898 Cuts at: 4142 6526 136 Size: 2384 1525 Fragments arranged by size: 2384 1525 1519 898 631 605 122 107 71 53 BglII A'GATC_T Cuts at: 7910 7910 Size: 7915 BlpI GC'TnA_GC Cuts at: 2054 4608 5332 2054 Size: 2554 724 4637 Fragments arranged by size: 4637 2554 724 Bme1580I G_kGCm'C Cuts at: 1790 2183 2215 2469 2930 3240 3573 3751 4082 Size: 393 32 254 461 310 333 178 331 Cuts at: 4082 4825 5140 5976 7132 1790 Size: 743 315 836 1156 2573 Fragments arranged by size: 2573 1156 836 743 461 393 333 331 315 310 254 178 32 BmgBI CAC'GTC Cuts at: 1318 2704 3964 1318 Size: 1386 1260 5269 Fragments arranged by size: 5269 1386 1260 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5646 6604 442 Size: 416 53 83 399 24 4229 958 1753 Fragments arranged by size: 4229 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7915 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2349 3293 4961 6575 1239 Size: 1110 944 1668 1614 2579 Fragments arranged by size: 2579 1668 1614 1110 944 Bpu10I CC'TnA_GC Cuts at: 2258 2580 4282 4449 4709 4856 5085 5141 2258 Size: 322 1702 167 260 147 229 56 5032 Fragments arranged by size: 5032 1702 322 260 229 167 147 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2674 3494 4637 6040 6905 7203 2674 Size: 820 1143 1403 865 298 3386 Fragments arranged by size: 3386 1403 1143 865 820 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2862 3849 4469 4641 6578 882 Size: 617 1363 987 620 172 1937 2219 Fragments arranged by size: 2219 1937 1363 987 620 617 172 BsaAI yAC'GTr Cuts at: 493 1204 5706 493 Size: 711 4502 2702 Fragments arranged by size: 4502 2702 711 BsaBI GATnn'nnATC Cuts at: 3356 3929 7909 3356 Size: 573 3980 3362 Fragments arranged by size: 3980 3362 573 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2143 5722 6104 6739 Size: 53 83 186 936 610 3579 382 635 Cuts at: 6739 275 Size: 1451 Fragments arranged by size: 3579 1451 936 635 610 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4533 6348 6746 7020 7167 7578 4533 Size: 1815 398 274 147 411 4870 Fragments arranged by size: 4870 1815 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2717 2747 4751 4781 5144 Size: 30 165 30 1314 30 2004 30 363 Cuts at: 5144 5174 1178 Size: 30 3919 Fragments arranged by size: 3919 2004 1314 363 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2738 3032 3290 4082 4244 4475 1186 Size: 1552 294 258 792 162 231 4626 Fragments arranged by size: 4626 1552 792 294 258 231 162 BseYI C'CCAG_C Cuts at: 2077 2754 3059 3460 3528 3659 3913 4162 4408 Size: 677 305 401 68 131 254 249 246 Cuts at: 4408 5066 5133 7118 7614 2077 Size: 658 67 1985 496 2378 Fragments arranged by size: 2378 1985 677 658 496 401 305 254 249 246 131 68 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7342 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5365 6126 7154 1540 Size: 275 186 3364 761 1028 2301 Fragments arranged by size: 3364 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3078 4764 5976 6061 7132 3078 Size: 1686 1212 85 1071 3861 Fragments arranged by size: 3861 1686 1212 1071 85 BsiWI C'GTAC_G Cuts at: 3803 3803 Size: 7915 BsmI GAATG_Cn' Cuts at: 4250 5426 5519 4250 Size: 1176 93 6646 Fragments arranged by size: 6646 1176 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2183 2215 2469 2930 3060 3078 3240 Size: 399 393 32 254 461 130 18 162 Cuts at: 3240 3573 3751 4082 4325 4614 4764 4825 5140 Size: 333 178 331 243 289 150 61 315 Cuts at: 5140 5234 5976 6061 7132 1391 Size: 94 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 461 399 393 333 331 315 289 254 243 178 162 150 130 94 85 61 32 18 BspEI T'CCGG_A Cuts at: 4533 7578 4533 Size: 3045 4870 BspHI T'CATG_A Cuts at: 5804 7534 5804 Size: 1730 6185 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2372 3038 3182 4001 5270 844 Size: 1528 666 144 819 1269 3489 Fragments arranged by size: 3489 1528 1269 819 666 144 BsrBI CCG'CTC Cuts at: 1394 1823 2233 2551 5802 1394 Size: 429 410 318 3251 3507 Fragments arranged by size: 3507 3251 429 410 318 BsrDI GCAATG_nn' Cuts at: 65 1248 2658 2737 2893 3714 3996 4152 6410 Size: 1183 1410 79 156 821 282 156 2258 Cuts at: 6410 6584 65 Size: 174 1396 Fragments arranged by size: 2258 1410 1396 1183 821 282 174 156 156 79 BsrFI r'CCGG_y Cuts at: 1700 1810 2040 2277 3464 5154 5212 6559 6746 Size: 110 230 237 1187 1690 58 1347 187 Cuts at: 6746 1700 Size: 2869 Fragments arranged by size: 2869 1690 1347 1187 237 230 187 110 58 BsrGI T'GTAC_A Cuts at: 96 4869 96 Size: 4773 3142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7915 BssSI C'ACGA_G Cuts at: 3125 5975 6987 3125 Size: 2850 1012 4053 Fragments arranged by size: 4053 2850 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4011 794 Size: 3217 4698 BstBI TT'CG_AA Cuts at: 6719 6719 Size: 7915 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7915 BstXI CCAn_nnnn'nTGG Cuts at: 2462 2941 3913 4118 5185 2462 Size: 479 972 205 1067 5192 Fragments arranged by size: 5192 1067 972 479 205 BstYI r'GATC_y Cuts at: 1972 2021 2421 2895 4290 4639 5245 5612 5997 Size: 49 400 474 1395 349 606 367 385 Cuts at: 5997 6014 7466 7552 7564 7910 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1395 606 474 400 385 367 349 346 86 49 17 12 Bsu36I CC'TnA_GG Cuts at: 3972 4664 3972 Size: 692 7223 BtgI C'CryG_G Cuts at: 513 1065 2004 2993 3100 3564 4332 4379 5023 Size: 552 939 989 107 464 768 47 644 Cuts at: 5023 5199 513 Size: 176 3229 Fragments arranged by size: 3229 989 939 768 644 552 464 176 107 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7176 Fragments arranged by size: 7176 683 56 BtsI GCAGTG_nn' Cuts at: 2132 2350 2780 3545 3845 5431 6225 6245 2132 Size: 218 430 765 300 1586 794 20 3802 Fragments arranged by size: 3802 1586 794 765 430 300 218 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7915 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2827 2862 529 Size: 35 2263 35 5582 Fragments arranged by size: 5582 2263 35 35 DraI TTT'AAA Cuts at: 5219 5219 Size: 7915 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6922 809 Size: 507 5606 1802 Fragments arranged by size: 5606 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2567 2623 Size: 54 1425 50 275 18 198 539 56 Cuts at: 2623 2879 2911 3138 3183 3228 4335 5362 6251 Size: 256 32 227 45 45 1107 1027 889 Cuts at: 6251 7880 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1107 1027 889 539 275 256 227 198 56 54 50 45 45 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 5362 1537 Size: 275 3550 4090 Fragments arranged by size: 4090 3550 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4409 4487 5845 1353 Size: 3056 78 1358 3423 Fragments arranged by size: 3423 3056 1358 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4883 6502 6876 7022 387 Size: 1064 138 3294 1619 374 146 1280 Fragments arranged by size: 3294 1619 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2349 2417 3269 3293 4961 5149 5978 Size: 393 717 68 852 24 1668 188 829 Cuts at: 5978 6575 7362 7630 1239 Size: 597 787 268 1524 Fragments arranged by size: 1668 1524 852 829 787 717 597 393 268 188 68 24 EcoNI CCTnn'n_nnAGG Cuts at: 3308 4442 4859 3308 Size: 1134 417 6364 Fragments arranged by size: 6364 1134 417 EcoO109I rG'GnC_Cy Cuts at: 2211 2212 2384 2502 2926 3396 3663 3917 4033 Size: 1 172 118 424 470 267 254 116 Cuts at: 4033 4078 4079 5137 2211 Size: 45 1 1058 4989 Fragments arranged by size: 4989 1058 470 424 267 254 172 118 116 45 1 1 EcoRI G'AATT_C Cuts at: 5224 5602 5224 Size: 378 7537 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2969 4390 2969 Size: 1421 6494 FspI TGC'GCA Cuts at: 3404 6421 3404 Size: 3017 4898 HaeII r_GCGC'y Cuts at: 1678 2146 2153 4192 5131 5184 7062 1678 Size: 468 7 2039 939 53 1878 2531 Fragments arranged by size: 2531 2039 1878 939 468 53 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3198 3230 4751 4783 5144 Size: 32 135 32 1626 32 1521 32 361 Cuts at: 5144 5176 6556 6588 6630 6662 1373 Size: 32 1380 32 42 32 2626 Fragments arranged by size: 2626 1626 1521 1380 361 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3217 5259 5505 669 Size: 988 281 1279 2042 246 3079 Fragments arranged by size: 3079 2042 1279 988 281 246 HindIII A'AGCT_T Cuts at: 748 2671 748 Size: 1923 5992 HpaI GTT'AAC Cuts at: 5505 5505 Size: 7915 KasI G'GCGC_C Cuts at: 2142 2142 Size: 7915 KpnI G_GTAC'C Cuts at: 2088 3442 5240 2088 Size: 1354 1798 4763 Fragments arranged by size: 4763 1798 1354 MfeI C'AATT_G Cuts at: 5514 5514 Size: 7915 MluI A'CGCG_T Cuts at: 7791 7791 Size: 7915 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3747 4053 4524 4794 7029 7213 1305 Size: 2442 306 471 270 2235 184 2007 Fragments arranged by size: 2442 2235 2007 471 306 270 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2625 2913 3140 3230 10 Size: 54 1425 343 793 288 227 90 4695 Fragments arranged by size: 4695 1425 793 343 288 227 90 54 MslI CAynn'nnrTG Cuts at: 518 1166 2460 3224 3538 4157 5873 6232 6391 Size: 648 1294 764 314 619 1716 359 159 Cuts at: 6391 518 Size: 2042 Fragments arranged by size: 2042 1716 1294 764 648 619 359 314 159 MspA1I CmG'CkG Cuts at: 1606 2074 2270 2573 2904 3008 3202 3271 3868 Size: 468 196 303 331 104 194 69 597 Cuts at: 3868 4011 4139 4334 4525 4912 5331 6008 7156 Size: 143 128 195 191 387 419 677 1148 Cuts at: 7156 7401 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 597 468 419 387 331 303 245 196 195 194 191 143 128 104 69 NaeI GCC'GGC Cuts at: 3466 3466 Size: 7915 NarI GG'CG_CC Cuts at: 2143 2143 Size: 7915 NcoI C'CATG_G Cuts at: 513 1065 2004 3100 5023 513 Size: 552 939 1096 1923 3405 Fragments arranged by size: 3405 1923 1096 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7915 NgoMIV G'CCGG_C Cuts at: 3464 3464 Size: 7915 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7915 NotI GC'GGCC_GC Cuts at: 5362 5362 Size: 7915 NspI r_CATG'y Cuts at: 1132 1229 2192 2980 5273 6818 1132 Size: 97 963 788 2293 1545 2229 Fragments arranged by size: 2293 2229 1545 963 788 97 PasI CC'CwG_GG Cuts at: 1348 2245 3850 4750 4849 5185 1348 Size: 897 1605 900 99 336 4078 Fragments arranged by size: 4078 1605 900 897 336 99 PciI A'CATG_T Cuts at: 2188 6814 2188 Size: 4626 3289 PflMI CCAn_nnn'nTGG Cuts at: 1108 2308 2704 3066 3130 3499 3964 4621 1108 Size: 1200 396 362 64 369 465 657 4402 Fragments arranged by size: 4402 1200 657 465 396 369 362 64 PfoI T'CCnGG_A Cuts at: 2220 2940 3897 4938 2220 Size: 720 957 1041 5197 Fragments arranged by size: 5197 1041 957 720 PmeI GTTT'AAAC Cuts at: 5219 5219 Size: 7915 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2717 2749 5984 6016 7497 7529 2717 Size: 32 3235 32 1481 32 3103 Fragments arranged by size: 3235 3103 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2384 2502 3396 3663 3917 2384 Size: 118 894 267 254 6382 Fragments arranged by size: 6382 894 267 254 118 PshAI GACnn'nnGTC Cuts at: 1613 2438 2873 1613 Size: 825 435 6655 Fragments arranged by size: 6655 825 435 PsiI TTA'TAA Cuts at: 5485 5485 Size: 7915 PspOMI G'GGCC_C Cuts at: 2179 2211 2926 4078 5136 2179 Size: 32 715 1152 1058 4958 Fragments arranged by size: 4958 1152 1058 715 32 PstI C_TGCA'G Cuts at: 830 2139 2286 2775 3840 4212 4395 4503 5267 Size: 1309 147 489 1065 372 183 108 764 Cuts at: 5267 830 Size: 3478 Fragments arranged by size: 3478 1309 1065 764 489 372 183 147 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7915 PvuII CAG'CTG Cuts at: 2270 2573 3008 3271 4011 4525 2270 Size: 303 435 263 740 514 5660 Fragments arranged by size: 5660 740 514 435 303 263 SacII CC_GC'GG Cuts at: 4335 4335 Size: 7915 SalI G'TCGA_C Cuts at: 1936 5257 1936 Size: 3321 4594 SanDI GG'GwC_CC Cuts at: 2502 3663 3917 2502 Size: 1161 254 6500 Fragments arranged by size: 6500 1161 254 SapI GCTCTTCn'nnn_ Cuts at: 4487 4487 Size: 7915 SbfI CC_TGCA'GG Cuts at: 5267 5267 Size: 7915 ScaI AGT'ACT Cuts at: 4883 5049 6163 7875 4883 Size: 166 1114 1712 4923 Fragments arranged by size: 4923 1712 1114 166 SfcI C'TryA_G Cuts at: 826 1045 2135 2282 2771 3327 3836 4208 4391 Size: 219 1090 147 489 556 509 372 183 Cuts at: 4391 4499 5263 6398 7079 7270 826 Size: 108 764 1135 681 191 1471 Fragments arranged by size: 1471 1135 1090 764 681 556 509 489 372 219 191 183 147 108 SfoI GGC'GCC Cuts at: 2144 2144 Size: 7915 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7915 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7915 SmaI CCC'GGG Cuts at: 1872 3680 5242 1872 Size: 1808 1562 4545 Fragments arranged by size: 4545 1808 1562 SmlI C'TyrA_G Cuts at: 820 1017 1943 2653 3509 3687 4652 6019 6920 Size: 197 926 710 856 178 965 1367 901 Cuts at: 6920 7182 820 Size: 262 1553 Fragments arranged by size: 1553 1367 965 926 901 856 710 262 197 178 SnaBI TAC'GTA Cuts at: 493 5706 493 Size: 5213 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7915 SphI G_CATG'C Cuts at: 1132 5273 1132 Size: 4141 3774 SspI AAT'ATT Cuts at: 5 52 3145 5839 5 Size: 47 3093 2694 2081 Fragments arranged by size: 3093 2694 2081 47 StuI AGG'CCT Cuts at: 1517 4901 1517 Size: 3384 4531 StyI C'CwwG_G Cuts at: 513 1065 2004 3042 3100 5023 5148 5354 513 Size: 552 939 1038 58 1923 125 206 3074 Fragments arranged by size: 3074 1923 1038 939 552 206 125 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2947 3113 3634 4121 4754 4894 5942 Size: 1255 1367 166 521 487 633 140 1048 Cuts at: 5942 5959 6112 6297 6742 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1367 1255 1048 633 521 487 445 185 166 153 140 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2193 4869 4881 Size: 274 80 33 51 1443 216 2676 12 Cuts at: 4881 5047 6161 7873 96 Size: 166 1114 1712 138 Fragments arranged by size: 2676 1712 1443 1114 274 216 166 138 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2922 2954 7875 7907 2922 Size: 32 4921 32 2930 Fragments arranged by size: 4921 2930 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7915 XbaI T'CTAG_A Cuts at: 5251 5251 Size: 7915 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2618 2686 4405 4723 2618 Size: 68 1719 318 5810 Fragments arranged by size: 5810 1719 318 68 XhoI C'TCGA_G Cuts at: 1943 3687 1943 Size: 1744 6171 XmaI C'CCGG_G Cuts at: 1870 3678 5240 1870 Size: 1808 1562 4545 Fragments arranged by size: 4545 1808 1562 XmnI GAAnn'nnTTC Cuts at: 6044 6044 Size: 7915 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5723 276 Size: 53 83 186 936 4189 2468 Fragments arranged by size: 4189 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BfrBI BplI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI