Restriction map for
pFN21AE3357
(Circular) (Six-base) MAPSORT of: pFN21AE3357.seq Check: 6567 from: 1 to: 7870
pFN21AE3357 7870 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5680 278
Size: 53 83 186 936 4144 2468
Fragments arranged by size:
4144 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4431 5213 1937
Size: 2494 782 4594
Fragments arranged by size:
4594 2494 782
Acc65I G'GTAC_C
Cuts at: 2705 5191 2705
Size: 2486 5384
AclI AA'CG_TT
Cuts at: 1563 5997 6370 1563
Size: 4434 373 3063
Fragments arranged by size:
4434 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 2871 2963 3344 3483 4593 5933 7317
Size: 662 577 92 381 139 1110 1340 1384
Cuts at: 7317 7585 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1340 1110 662 577 381 268
139 92
AflII C'TTAA_G
Cuts at: 820 1017 2750 820
Size: 197 1733 5940
Fragments arranged by size:
5940 1733 197
AflIII A'CryG_T
Cuts at: 1203 6769 7746 1203
Size: 5566 977 1327
Fragments arranged by size:
5566 1327 977
AgeI A'CCGG_T
Cuts at: 6701 6701
Size: 7870
AhdI GACnn_n'nnGTC
Cuts at: 6599 6599
Size: 7870
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7870
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4989 5021 4989
Size: 32 7838
AlwNI CAG_nnn'CTG
Cuts at: 3533 5613 7185 3533
Size: 2080 1572 4218
Fragments arranged by size:
4218 2080 1572
ApaLI G'TGCA_C
Cuts at: 5927 7083 5927
Size: 1156 6714
ApoI r'AATT_y
Cuts at: 1493 2579 2656 2745 2942 3061 3137 3168 3185
Size: 1086 77 89 197 119 76 31 17
Cuts at: 3185 3374 4501 4622 4663 5179 5405 5557 7666
Size: 189 1127 121 41 516 226 152 2109
Cuts at: 7666 1493
Size: 1697
Fragments arranged by size:
2109 1697 1127 1086 516 226 197 189
152 121 119 89 77 76 41 31
17
AseI AT'TA_AT
Cuts at: 160 1032 2094 3740 6424 160
Size: 872 1062 1646 2684 1606
Fragments arranged by size:
2684 1646 1606 1062 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7870
AvaI C'yCGr_G
Cuts at: 1870 1943 5195 1870
Size: 73 3252 4545
Fragments arranged by size:
4545 3252 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2233 2259 2266 2292 2233
Size: 26 7 26 7811
Fragments arranged by size:
7811 26 26 7
BamHI G'GATC_C
Cuts at: 5200 5567 5200
Size: 367 7503
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2705 3610 4450 5191 6646
Size: 325 217 627 918 905 840 741 1455
Cuts at: 6646 618
Size: 1842
Fragments arranged by size:
1842 1455 918 905 840 741 627 325
217
BanII G_rGCy'C
Cuts at: 1391 3493 5189 1391
Size: 2102 1696 4072
Fragments arranged by size:
4072 2102 1696
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3163 4083 4392 928
Size: 971 1264 920 309 4406
Fragments arranged by size:
4406 1264 971 920 309
BbvCI CC'TCA_GC
Cuts at: 2190 2678 2190
Size: 488 7382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6061 6095 1903
Size: 34 4124 34 3678
Fragments arranged by size:
4124 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3576 5762 6972 3576
Size: 2186 1210 4474
Fragments arranged by size:
4474 2186 1210
BclI T'GATC_A
Cuts at: 1548 3844 1548
Size: 2296 5574
BfrBI ATG'CAT
Cuts at: 3368 3604 3368
Size: 236 7634
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6481 136
Size: 107 122 71 631 605 4809 1525
Fragments arranged by size:
4809 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2998 7865 2998
Size: 4867 3003
BlpI GC'TnA_GC
Cuts at: 5287 5287
Size: 7870
Bme1580I G_kGCm'C
Cuts at: 1790 3825 5931 7087 1790
Size: 2035 2106 1156 2573
Fragments arranged by size:
2573 2106 2035 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7870
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3838 4026 5601
Size: 416 53 83 399 24 2421 188 1575
Cuts at: 5601 6559 442
Size: 958 1753
Fragments arranged by size:
2421 1753 1575 958 416 399 188 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7870
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2484 2484
Size: 7870
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2310 3573 3688 4860 6530 1239
Size: 1071 1263 115 1172 1670 2579
Fragments arranged by size:
2579 1670 1263 1172 1071 115
Bpu10I CC'TnA_GC
Cuts at: 2190 2678 2190
Size: 488 7382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2347 2607 3367 4530 5995 6860 7158 2347
Size: 260 760 1163 1465 865 298 3059
Fragments arranged by size:
3059 1465 1163 865 760 298 260
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4206 6533 882
Size: 617 2707 2327 2219
Fragments arranged by size:
2707 2327 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5661 493
Size: 711 4457 2702
Fragments arranged by size:
4457 2702 711
BsaBI GATnn'nnATC
Cuts at: 7864 7864
Size: 7870
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5677 6059 6694 275
Size: 53 83 186 936 4144 382 635 1451
Fragments arranged by size:
4144 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3326 4051 6303 6701 6975 7122 7533 3326
Size: 725 2252 398 274 147 411 3663
Fragments arranged by size:
3663 2252 725 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4989 5019 1178
Size: 30 165 30 3586 30 4029
Fragments arranged by size:
4029 3586 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2870 4013 4613 1186
Size: 1027 657 1143 600 4443
Fragments arranged by size:
4443 1143 1027 657 600
BseYI C'CCAG_C
Cuts at: 3818 7073 7569 3818
Size: 3255 496 4119
Fragments arranged by size:
4119 3255 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5320 6081 7109 1540
Size: 275 186 3319 761 1028 2301
Fragments arranged by size:
3319 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5931 6016 7087 5931
Size: 85 1071 6714
Fragments arranged by size:
6714 1071 85
BsmI GAATG_Cn'
Cuts at: 2182 2351 5381 5474 2182
Size: 169 3030 93 4578
Fragments arranged by size:
4578 3030 169 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2852 4164 2852
Size: 1312 6558
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3493 3825 5189 5931 6016 7087 1391
Size: 399 1703 332 1364 742 85 1071 2174
Fragments arranged by size:
2174 1703 1364 1071 742 399 332 85
BspEI T'CCGG_A
Cuts at: 7533 7533
Size: 7870
BspHI T'CATG_A
Cuts at: 4875 5759 7489 4875
Size: 884 1730 5256
Fragments arranged by size:
5256 1730 884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5225 844
Size: 4381 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5757 1394
Size: 429 3934 3507
Fragments arranged by size:
3934 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2239 3030 6365 6539 65
Size: 1183 991 791 3335 174 1396
Fragments arranged by size:
3335 1396 1183 991 791 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6514 6701 1700
Size: 110 4704 187 2869
Fragments arranged by size:
4704 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3963 96
Size: 3867 4003
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7870
BssSI C'ACGA_G
Cuts at: 5930 6942 5930
Size: 1012 6858
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2023 4124 794
Size: 1229 2101 4540
Fragments arranged by size:
4540 2101 1229
BstBI TT'CG_AA
Cuts at: 4833 6674 4833
Size: 1841 6029
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7870
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 7870
BstYI r'GATC_y
Cuts at: 1972 2998 5200 5567 5952 5969 7421 7507 7519
Size: 1026 2202 367 385 17 1452 86 12
Cuts at: 7519 7865 1972
Size: 346 1977
Fragments arranged by size:
2202 1977 1452 1026 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2010 2886 513
Size: 552 945 876 5497
Fragments arranged by size:
5497 945 876 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3647 487
Size: 683 56 2421 4710
Fragments arranged by size:
4710 2421 683 56
BtsI GCAGTG_nn'
Cuts at: 2160 5386 6180 6200 2160
Size: 3226 794 20 3830
Fragments arranged by size:
3830 3226 794 20
ClaI AT'CG_AT
Cuts at: 1554 3778 4966 1554
Size: 2224 1188 4458
Fragments arranged by size:
4458 2224 1188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4038 4073 529
Size: 35 3474 35 4326
Fragments arranged by size:
4326 3474 35 35
DraI TTT'AAA
Cuts at: 5174 5174
Size: 7870
DraIII CAC_nnn'GTG
Cuts at: 3440 3440
Size: 7870
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6877 809
Size: 507 5561 1802
Fragments arranged by size:
5561 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2334 5317 6206
Size: 54 1425 50 275 18 504 2983 889
Cuts at: 6206 7835 8
Size: 1629 43
Fragments arranged by size:
2983 1629 1425 889 504 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5317 1537
Size: 275 3505 4090
Fragments arranged by size:
4090 3505 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2432 2847 2862 3249 3900 4783 4835 5161
Size: 1079 415 15 387 651 883 52 326
Cuts at: 5161 5800 1353
Size: 639 3423
Fragments arranged by size:
3423 1079 883 651 639 415 387 326
52 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6457 6831 6977 387
Size: 1064 138 4868 374 146 1280
Fragments arranged by size:
4868 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2310 2871 2963 3344 3483 3573
Size: 393 662 16 561 92 381 139 90
Cuts at: 3573 3688 4593 4860 5933 6530 7317 7585 1239
Size: 115 905 267 1073 597 787 268 1524
Fragments arranged by size:
1524 1073 905 787 662 597 561 393
381 268 267 139 115 92 90 16
EcoRI G'AATT_C
Cuts at: 3061 5179 5557 3061
Size: 2118 378 5374
Fragments arranged by size:
5374 2118 378
EcoRV GAT'ATC
Cuts at: 4776 4776
Size: 7870
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2324 2324
Size: 7870
FspI TGC'GCA
Cuts at: 6376 6376
Size: 7870
HaeII r_GCGC'y
Cuts at: 1678 7017 1678
Size: 5339 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4220 4252 6511 6543 6585
Size: 32 135 32 2648 32 2259 32 42
Cuts at: 6585 6617 1373
Size: 32 2626
Fragments arranged by size:
2648 2626 2259 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3412 5214 5460 669
Size: 988 281 1176 298 1802 246 3079
Fragments arranged by size:
3079 1802 1176 988 298 281 246
HindIII A'AGCT_T
Cuts at: 748 4134 4148 748
Size: 3386 14 4470
Fragments arranged by size:
4470 3386 14
HpaI GTT'AAC
Cuts at: 3114 5460 3114
Size: 2346 5524
KpnI G_GTAC'C
Cuts at: 2709 5195 2709
Size: 2486 5384
MfeI C'AATT_G
Cuts at: 2319 3732 5469 2319
Size: 1413 1737 4720
Fragments arranged by size:
4720 1737 1413
MluI A'CGCG_T
Cuts at: 7746 7746
Size: 7870
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4332 6984 7168 1305
Size: 3027 2652 184 2007
Fragments arranged by size:
3027 2652 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6048
Fragments arranged by size:
6048 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2249 2957 4305 5828 6187 6346
Size: 648 843 240 708 1348 1523 359 159
Cuts at: 6346 518
Size: 2042
Fragments arranged by size:
2042 1523 1348 843 708 648 359 240
159
MspA1I CmG'CkG
Cuts at: 1606 5286 5963 7111 7356 1606
Size: 3680 677 1148 245 2120
Fragments arranged by size:
3680 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2886 513
Size: 552 1821 5497
Fragments arranged by size:
5497 1821 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7870
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7870
NotI GC'GGCC_GC
Cuts at: 5317 5317
Size: 7870
NsiI A_TGCA'T
Cuts at: 3370 3606 3370
Size: 236 7634
NspI r_CATG'y
Cuts at: 1132 1229 2433 3220 3788 5228 6773 1132
Size: 97 1204 787 568 1440 1545 2229
Fragments arranged by size:
2229 1545 1440 1204 787 568 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7870
PciI A'CATG_T
Cuts at: 6769 6769
Size: 7870
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3440 1108
Size: 2332 5538
PfoI T'CCnGG_A
Cuts at: 3578 3578
Size: 7870
PmeI GTTT'AAAC
Cuts at: 5174 5174
Size: 7870
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4989 5021 5939 5971 7452 7484 4989
Size: 32 918 32 1481 32 5375
Fragments arranged by size:
5375 1481 918 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7870
PsiI TTA'TAA
Cuts at: 5440 5440
Size: 7870
PstI C_TGCA'G
Cuts at: 830 2031 5222 830
Size: 1201 3191 3478
Fragments arranged by size:
3478 3191 1201
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7870
SalI G'TCGA_C
Cuts at: 1936 5212 1936
Size: 3276 4594
SapI GCTCTTCn'nnn_
Cuts at: 3900 3900
Size: 7870
SbfI CC_TGCA'GG
Cuts at: 5222 5222
Size: 7870
ScaI AGT'ACT
Cuts at: 2928 6118 7830 2928
Size: 3190 1712 2968
Fragments arranged by size:
3190 2968 1712
SfcI C'TryA_G
Cuts at: 826 1045 2027 2111 2448 4947 5218 6353 7034
Size: 219 982 84 337 2499 271 1135 681
Cuts at: 7034 7225 826
Size: 191 1471
Fragments arranged by size:
2499 1471 1135 982 681 337 271 219
191 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7870
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7870
SmaI CCC'GGG
Cuts at: 1872 5197 1872
Size: 3325 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2326 2622 2750 3382 4545 5974
Size: 197 926 383 296 128 632 1163 1429
Cuts at: 5974 6875 7137 820
Size: 901 262 1553
Fragments arranged by size:
1553 1429 1163 926 901 632 383 296
262 197 128
SnaBI TAC'GTA
Cuts at: 493 5661 493
Size: 5168 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7870
SphI G_CATG'C
Cuts at: 1132 5228 1132
Size: 4096 3774
SspI AAT'ATT
Cuts at: 5 52 5794 5
Size: 47 5742 2081
Fragments arranged by size:
5742 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7870
StyI C'CwwG_G
Cuts at: 513 1065 2886 3345 3614 4290 4981 5309 513
Size: 552 1821 459 269 676 691 328 3074
Fragments arranged by size:
3074 1821 691 676 552 459 328 269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 5897 5914 6067 6252 6697 325
Size: 1255 537 3780 17 153 185 445 1498
Fragments arranged by size:
3780 1498 1255 537 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2926 3744 3870
Size: 274 80 33 51 1443 949 818 126
Cuts at: 3870 3963 6116 7828 96
Size: 93 2153 1712 138
Fragments arranged by size:
2153 1712 1443 949 818 274 138 126
93 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7830 7862 7830
Size: 32 7838
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7870
XbaI T'CTAG_A
Cuts at: 4225 5206 4225
Size: 981 6889
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3815 3815
Size: 7870
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7870
XmaI C'CCGG_G
Cuts at: 1870 5195 1870
Size: 3325 4545
XmnI GAAnn'nnTTC
Cuts at: 3168 4275 4665 5999 3168
Size: 1107 390 1334 5039
Fragments arranged by size:
5039 1334 1107 390
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5678 276
Size: 53 83 186 936 4144 2468
Fragments arranged by size:
4144 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BsiWI BstZ17I
Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI