Restriction map for
pFN21AE3356
(Circular) (Six-base) MAPSORT of: pFN21AE3356.seq Check: 9719 from: 1 to: 7849
pFN21AE3356 7849 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3727 3727
Size: 7849
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5659 278
Size: 53 83 186 936 4123 2468
Fragments arranged by size:
4123 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4258 5192 1937
Size: 2321 934 4594
Fragments arranged by size:
4594 2321 934
Acc65I G'GTAC_C
Cuts at: 2738 5170 2738
Size: 2432 5417
AclI AA'CG_TT
Cuts at: 1563 5976 6349 1563
Size: 4413 373 3063
Fragments arranged by size:
4413 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3348 3618 3651 4404 4478 4784 5912 7296
Size: 1716 270 33 753 74 306 1128 1384
Cuts at: 7296 7564 1632
Size: 268 1917
Fragments arranged by size:
1917 1716 1384 1128 753 306 270 268
74 33
AfeI AGC'GCT
Cuts at: 3772 3772
Size: 7849
AflII C'TTAA_G
Cuts at: 820 1017 3731 820
Size: 197 2714 4938
Fragments arranged by size:
4938 2714 197
AflIII A'CryG_T
Cuts at: 1203 2251 2481 3260 6748 7725 1203
Size: 1048 230 779 3488 977 1327
Fragments arranged by size:
3488 1327 1048 977 779 230
AgeI A'CCGG_T
Cuts at: 6680 6680
Size: 7849
AhdI GACnn_n'nnGTC
Cuts at: 6578 6578
Size: 7849
AleI CACnn'nnGTG
Cuts at: 1166 5000 1166
Size: 3834 4015
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4039 1184
Size: 2855 4994
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2192 2224 2338 2370 2192
Size: 32 114 32 7671
Fragments arranged by size:
7671 114 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4195 4900 5592 7164 4195
Size: 705 692 1572 4880
Fragments arranged by size:
4880 1572 705 692
ApaLI G'TGCA_C
Cuts at: 2618 2714 5906 7062 2618
Size: 96 3192 1156 3405
Fragments arranged by size:
3405 3192 1156 96
ApoI r'AATT_y
Cuts at: 1493 2384 3509 3670 4066 4736 5104 5158 5384
Size: 891 1125 161 396 670 368 54 226
Cuts at: 5384 5536 7645 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1125 891 670 396 368 226
161 152 54
AseI AT'TA_AT
Cuts at: 160 1032 6403 160
Size: 872 5371 1606
Fragments arranged by size:
5371 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7849
AvaI C'yCGr_G
Cuts at: 1870 1943 2785 4574 4799 5174 1870
Size: 73 842 1789 225 375 4545
Fragments arranged by size:
4545 1789 842 375 225 73
BamHI G'GATC_C
Cuts at: 3094 5179 5546 3094
Size: 2085 367 5397
Fragments arranged by size:
5397 2085 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2738 3692 4681 5170 6625
Size: 325 217 627 951 954 989 489 1455
Cuts at: 6625 618
Size: 1842
Fragments arranged by size:
1842 1455 989 954 951 627 489 325
217
BanII G_rGCy'C
Cuts at: 1391 4084 4391 4749 4928 5168 1391
Size: 2693 307 358 179 240 4072
Fragments arranged by size:
4072 2693 358 307 240 179
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2244 3336 3542 928
Size: 971 345 1092 206 5235
Fragments arranged by size:
5235 1092 971 345 206
BbvCI CC'TCA_GC
Cuts at: 2868 2868
Size: 7849
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3018 3052 3575 3609 5010
Size: 34 50 34 997 34 523 34 1401
Cuts at: 5010 5044 6040 6074 1903
Size: 34 996 34 3678
Fragments arranged by size:
3678 1401 997 996 523 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4896 5741 6951 4896
Size: 845 1210 5794
Fragments arranged by size:
5794 1210 845
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7849
BfrBI ATG'CAT
Cuts at: 2862 2862
Size: 7849
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6460 136
Size: 107 122 71 631 605 4788 1525
Fragments arranged by size:
4788 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7844 7844
Size: 7849
BlpI GC'TnA_GC
Cuts at: 5266 5266
Size: 7849
Bme1580I G_kGCm'C
Cuts at: 1790 2622 2718 4684 5910 7066 1790
Size: 832 96 1966 1226 1156 2573
Fragments arranged by size:
2573 1966 1226 1156 832 96
BmgBI CAC'GTC
Cuts at: 1318 3261 1318
Size: 1943 5906
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3102 5580 6538
Size: 416 53 83 399 24 1685 2478 958
Cuts at: 6538 442
Size: 1753
Fragments arranged by size:
2478 1753 1685 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7849
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 7849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3169 4217 4912 6509 1239
Size: 1930 1048 695 1597 2579
Fragments arranged by size:
2579 1930 1597 1048 695
Bpu10I CC'TnA_GC
Cuts at: 2868 4922 2868
Size: 2054 5795
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2836 5974 6839 7137 2836
Size: 3138 865 298 3548
Fragments arranged by size:
3548 3138 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2812 4004 6512 882
Size: 617 1313 1192 2508 2219
Fragments arranged by size:
2508 2219 1313 1192 617
BsaAI yAC'GTr
Cuts at: 493 1204 5640 493
Size: 711 4436 2702
Fragments arranged by size:
4436 2702 711
BsaBI GATnn'nnATC
Cuts at: 7843 7843
Size: 7849
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5656 6038 6673 275
Size: 53 83 186 936 4123 382 635 1451
Fragments arranged by size:
4123 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3204 3955 4265 4555 6282 6680 6954 7101 7512
Size: 751 310 290 1727 398 274 147 411
Cuts at: 7512 3204
Size: 3541
Fragments arranged by size:
3541 1727 751 411 398 310 290 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2338 2368 2495 2525 2746
Size: 30 165 30 935 30 127 30 221
Cuts at: 2746 2776 3250 3280 3937 3967 1178
Size: 30 474 30 657 30 5060
Fragments arranged by size:
5060 935 657 474 221 165 127 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2629 3939 3942 1186
Size: 1443 1310 3 5093
Fragments arranged by size:
5093 1443 1310 3
BseYI C'CCAG_C
Cuts at: 4285 7052 7548 4285
Size: 2767 496 4586
Fragments arranged by size:
4586 2767 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3048 4536 1163
Size: 573 1312 1488 4476
Fragments arranged by size:
4476 1488 1312 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2628 5299 6060 7088 1540
Size: 275 186 627 2671 761 1028 2301
Fragments arranged by size:
2671 2301 1028 761 627 275 186
BsiHKAI G_wGCw'C
Cuts at: 2622 2706 2718 2969 4928 5910 5995 7066 2622
Size: 84 12 251 1959 982 85 1071 3405
Fragments arranged by size:
3405 1959 1071 982 251 85 84 12
BsmI GAATG_Cn'
Cuts at: 2781 5122 5360 5453 2781
Size: 2341 238 93 5177
Fragments arranged by size:
5177 2341 238 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2622 2706 2718 2969 4084 4391 4684
Size: 399 832 84 12 251 1115 307 293
Cuts at: 4684 4749 4928 5168 5910 5995 7066 1391
Size: 65 179 240 742 85 1071 2174
Fragments arranged by size:
2174 1115 1071 832 742 399 307 293
251 240 179 85 84 65 12
BspEI T'CCGG_A
Cuts at: 3955 7512 3955
Size: 3557 4292
BspHI T'CATG_A
Cuts at: 5738 7468 5738
Size: 1730 6119
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3727 5204 844
Size: 2883 1477 3489
Fragments arranged by size:
3489 2883 1477
BsrBI CCG'CTC
Cuts at: 1394 1823 5736 1394
Size: 429 3913 3507
Fragments arranged by size:
3913 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6344 6518 65
Size: 1183 5096 174 1396
Fragments arranged by size:
5096 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2268 3212 6493 6680 1700
Size: 110 458 944 3281 187 2869
Fragments arranged by size:
3281 2869 944 458 187 110
BsrGI T'GTAC_A
Cuts at: 96 2052 96
Size: 1956 5893
BssHII G'CGCG_C
Cuts at: 1925 2748 1925
Size: 823 7026
BssSI C'ACGA_G
Cuts at: 5909 6921 5909
Size: 1012 6837
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2211 4900 794
Size: 1417 2689 3743
Fragments arranged by size:
3743 2689 1417
BstBI TT'CG_AA
Cuts at: 6653 6653
Size: 7849
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7849
BstXI CCAn_nnnn'nTGG
Cuts at: 3238 3238
Size: 7849
BstYI r'GATC_y
Cuts at: 1972 2436 3094 3335 5179 5546 5931 5948 7400
Size: 464 658 241 1844 367 385 17 1452
Cuts at: 7400 7486 7498 7844 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1844 1452 658 464 385 367 346
241 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3067 3084 3303 4074 4676 513
Size: 552 2002 17 219 771 602 3686
Fragments arranged by size:
3686 2002 771 602 552 219 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7110
Fragments arranged by size:
7110 683 56
BtsI GCAGTG_nn'
Cuts at: 3130 5365 6159 6179 3130
Size: 2235 794 20 4800
Fragments arranged by size:
4800 2235 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7849
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7814
DraI TTT'AAA
Cuts at: 4065 5153 4065
Size: 1088 6761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6856 809
Size: 507 5540 1802
Fragments arranged by size:
5540 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2625 5296 6185
Size: 54 1425 50 275 18 795 2671 889
Cuts at: 6185 7814 8
Size: 1629 43
Fragments arranged by size:
2671 1629 1425 889 795 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2625 5296 1537
Size: 275 813 2671 4090
Fragments arranged by size:
4090 2671 813 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4089 4599 4687 4810 5779 1353
Size: 2736 510 88 123 969 3423
Fragments arranged by size:
3423 2736 969 510 123 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3953 6436 6810 6956 387
Size: 1064 138 2364 2483 374 146 1280
Fragments arranged by size:
2483 2364 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4926 4926
Size: 7849
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3169 3348 3618 3651 4217 4404 4478
Size: 393 1537 179 270 33 566 187 74
Cuts at: 4478 4784 4912 5912 6509 7296 7564 1239
Size: 306 128 1000 597 787 268 1524
Fragments arranged by size:
1537 1524 1000 787 597 566 393 306
270 268 187 179 128 74 33
EcoNI CCTnn'n_nnAGG
Cuts at: 4398 4398
Size: 7849
EcoO109I rG'GnC_Cy
Cuts at: 4408 4408
Size: 7849
EcoRI G'AATT_C
Cuts at: 5158 5536 5158
Size: 378 7471
FspI TGC'GCA
Cuts at: 3660 6355 3660
Size: 2695 5154
HaeII r_GCGC'y
Cuts at: 1678 3080 3774 4901 6996 1678
Size: 1402 694 1127 2095 2531
Fragments arranged by size:
2531 2095 1402 1127 694
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2746 2778 3250
Size: 32 135 32 530 32 612 32 472
Cuts at: 3250 3282 4150 4182 6490 6522 6564 6596 1373
Size: 32 868 32 2308 32 42 32 2626
Fragments arranged by size:
2626 2308 868 612 530 472 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4259 4792 5193 5439 669
Size: 988 281 2321 533 401 246 3079
Fragments arranged by size:
3079 2321 988 533 401 281 246
HindIII A'AGCT_T
Cuts at: 748 3150 748
Size: 2402 5447
HpaI GTT'AAC
Cuts at: 5439 5439
Size: 7849
KpnI G_GTAC'C
Cuts at: 2742 5174 2742
Size: 2432 5417
MfeI C'AATT_G
Cuts at: 4090 5448 4090
Size: 1358 6491
MluI A'CGCG_T
Cuts at: 7725 7725
Size: 7849
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6963 7147 1305
Size: 5658 184 2007
Fragments arranged by size:
5658 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6027
Fragments arranged by size:
6027 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3236 3486 3502 3816 5000 5807 6166
Size: 648 2070 250 16 314 1184 807 359
Cuts at: 6166 6325 518
Size: 159 2042
Fragments arranged by size:
2070 2042 1184 807 648 359 314 250
159 16
MspA1I CmG'CkG
Cuts at: 1606 2135 2905 3172 3650 3824 4019 5265 5942
Size: 529 770 267 478 174 195 1246 677
Cuts at: 5942 7090 7335 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1246 1148 770 677 529 478 267
245 195 174
NaeI GCC'GGC
Cuts at: 3214 3214
Size: 7849
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7297
NdeI CA'TA_TG
Cuts at: 387 4842 387
Size: 4455 3394
NgoMIV G'CCGG_C
Cuts at: 3212 3212
Size: 7849
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7849
NotI GC'GGCC_GC
Cuts at: 5296 5296
Size: 7849
NsiI A_TGCA'T
Cuts at: 2864 2864
Size: 7849
NspI r_CATG'y
Cuts at: 1132 1229 2032 2209 2255 2485 2530 5207 6752
Size: 97 803 177 46 230 45 2677 1545
Cuts at: 6752 1132
Size: 2229
Fragments arranged by size:
2677 2229 1545 803 230 177 97 46
45
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7849
PciI A'CATG_T
Cuts at: 2251 2481 6748 2251
Size: 230 4267 3352
Fragments arranged by size:
4267 3352 230
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3172 4195 1108
Size: 2064 1023 4762
Fragments arranged by size:
4762 2064 1023
PfoI T'CCnGG_A
Cuts at: 4687 4687
Size: 7849
PmeI GTTT'AAAC
Cuts at: 5153 5153
Size: 7849
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2338 2370 5918 5950 7431 7463 2338
Size: 32 3548 32 1481 32 2724
Fragments arranged by size:
3548 2724 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4765 1613
Size: 3152 4697
PsiI TTA'TAA
Cuts at: 5419 5419
Size: 7849
PspXI vC'TCGA_Gb
Cuts at: 2785 2785
Size: 7849
PstI C_TGCA'G
Cuts at: 830 2216 2571 2805 3529 4028 4732 5201 830
Size: 1386 355 234 724 499 704 469 3478
Fragments arranged by size:
3478 1386 724 704 499 469 355 234
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7849
PvuII CAG'CTG
Cuts at: 2905 3172 3824 4019 2905
Size: 267 652 195 6735
Fragments arranged by size:
6735 652 267 195
SacI G_AGCT'C
Cuts at: 4928 4928
Size: 7849
SalI G'TCGA_C
Cuts at: 1936 4257 5191 1936
Size: 2321 934 4594
Fragments arranged by size:
4594 2321 934
SapI GCTCTTCn'nnn_
Cuts at: 4089 4089
Size: 7849
SbfI CC_TGCA'GG
Cuts at: 5201 5201
Size: 7849
ScaI AGT'ACT
Cuts at: 6097 7809 6097
Size: 1712 6137
SfcI C'TryA_G
Cuts at: 826 1045 2212 2567 2801 3525 3925 4024 4728
Size: 219 1167 355 234 724 400 99 704
Cuts at: 4728 5197 6332 7013 7204 826
Size: 469 1135 681 191 1471
Fragments arranged by size:
1471 1167 1135 724 704 681 469 400
355 234 219 191 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7849
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7849
SmaI CCC'GGG
Cuts at: 1872 4801 5176 1872
Size: 2929 375 4545
Fragments arranged by size:
4545 2929 375
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2785 2815 3731 5953 6854 7116
Size: 197 926 842 30 916 2222 901 262
Cuts at: 7116 820
Size: 1553
Fragments arranged by size:
2222 1553 926 916 901 842 262 197
30
SnaBI TAC'GTA
Cuts at: 493 5640 493
Size: 5147 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7849
SphI G_CATG'C
Cuts at: 1132 5207 1132
Size: 4075 3774
SspI AAT'ATT
Cuts at: 5 52 3466 5773 5
Size: 47 3414 2307 2081
Fragments arranged by size:
3414 2307 2081 47
StuI AGG'CCT
Cuts at: 1517 3033 5050 1517
Size: 1516 2017 4316
Fragments arranged by size:
4316 2017 1516
StyI C'CwwG_G
Cuts at: 513 1065 2196 2358 2507 3933 4492 4913 5288
Size: 552 1131 162 149 1426 559 421 375
Cuts at: 5288 513
Size: 3074
Fragments arranged by size:
3074 1426 1131 559 552 421 375 162
149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3986 5876 5893 6046 6231 6676 325
Size: 1255 2406 1890 17 153 185 445 1498
Fragments arranged by size:
2406 1890 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 6095 7807
Size: 274 80 33 51 1443 75 4043 1712
Cuts at: 7807 96
Size: 138
Fragments arranged by size:
4043 1712 1443 274 138 80 75 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4281 4313 7809 7841 4281
Size: 32 3496 32 4289
Fragments arranged by size:
4289 3496 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7849
XbaI T'CTAG_A
Cuts at: 5185 5185
Size: 7849
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3235 4840 3235
Size: 1605 6244
XhoI C'TCGA_G
Cuts at: 1943 2785 1943
Size: 842 7007
XmaI C'CCGG_G
Cuts at: 1870 4799 5174 1870
Size: 2929 375 4545
Fragments arranged by size:
4545 2929 375
XmnI GAAnn'nnTTC
Cuts at: 5978 5978
Size: 7849
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5657 276
Size: 53 83 186 936 4123 2468
Fragments arranged by size:
4123 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI
NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI