Restriction map for
pFN21AE3355
(Circular) (Six-base) MAPSORT of: pFN21AE3355.seq Check: 1645 from: 1 to: 7846
pFN21AE3355 7846 bp
With 182 enzymes: SgfI *
August 27, 2018 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2036 2036
Size: 7846
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4125 5656 278
Size: 53 83 186 936 2589 1531 2468
Fragments arranged by size:
2589 2468 1531 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4673 4710 5189 1937
Size: 2736 37 479 4594
Fragments arranged by size:
4594 2736 479 37
Acc65I G'GTAC_C
Cuts at: 5167 5167
Size: 7846
AclI AA'CG_TT
Cuts at: 1563 5973 6346 1563
Size: 4410 373 3063
Fragments arranged by size:
4410 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2702 3060 4515 4579 4940 5015 5909 7293
Size: 1070 358 1455 64 361 75 894 1384
Cuts at: 7293 7561 1632
Size: 268 1917
Fragments arranged by size:
1917 1455 1384 1070 894 361 358 268
75 64
AfeI AGC'GCT
Cuts at: 2254 4992 2254
Size: 2738 5108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7649
AflIII A'CryG_T
Cuts at: 1203 2804 3658 6745 7722 1203
Size: 1601 854 3087 977 1327
Fragments arranged by size:
3087 1601 1327 977 854
AgeI A'CCGG_T
Cuts at: 3623 6677 3623
Size: 3054 4792
AhdI GACnn_n'nnGTC
Cuts at: 6575 6575
Size: 7846
AleI CACnn'nnGTG
Cuts at: 1166 3531 1166
Size: 2365 5481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4658 1184
Size: 3474 4372
AlwNI CAG_nnn'CTG
Cuts at: 3697 5589 7161 3697
Size: 1892 1572 4382
Fragments arranged by size:
4382 1892 1572
ApaI G_GGCC'C
Cuts at: 2633 2984 2633
Size: 351 7495
ApaLI G'TGCA_C
Cuts at: 5903 7059 5903
Size: 1156 6690
ApoI r'AATT_y
Cuts at: 1493 2927 5155 5381 5533 7642 1493
Size: 1434 2228 226 152 2109 1697
Fragments arranged by size:
2228 2109 1697 1434 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3876 6400 160
Size: 872 2844 2524 1606
Fragments arranged by size:
2844 2524 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7846
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2616 2633 2841 2955 3075 3238
Size: 73 120 553 17 208 114 120 163
Cuts at: 3238 5171 1870
Size: 1933 4545
Fragments arranged by size:
4545 1933 553 208 163 120 120 114
73 17
BamHI G'GATC_C
Cuts at: 3024 3566 5176 5543 3024
Size: 542 1610 367 5327
Fragments arranged by size:
5327 1610 542 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2222 2242 2349 3071 3755
Size: 325 217 627 435 20 107 722 684
Cuts at: 3755 4385 5167 6622 618
Size: 630 782 1455 1842
Fragments arranged by size:
1842 1455 782 722 684 630 627 435
325 217 107 20
BanII G_rGCy'C
Cuts at: 1391 2633 2956 2984 3298 4315 5165 1391
Size: 1242 323 28 314 1017 850 4072
Fragments arranged by size:
4072 1242 1017 850 323 314 28
BbeI G_GCGC'C
Cuts at: 2226 2353 3759 2226
Size: 127 1406 6313
Fragments arranged by size:
6313 1406 127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2749 2967 928
Size: 971 850 218 5807
Fragments arranged by size:
5807 971 850 218
BbvCI CC'TCA_GC
Cuts at: 4196 4566 4865 4196
Size: 370 299 7177
Fragments arranged by size:
7177 370 299
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2816 2850 6037 6071 1903
Size: 34 879 34 3187 34 3678
Fragments arranged by size:
3678 3187 879 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5738 6948 5738
Size: 1210 6636
BclI T'GATC_A
Cuts at: 1548 2889 1548
Size: 1341 6505
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2327 4761 6457
Size: 107 122 71 631 605 655 2434 1696
Cuts at: 6457 136
Size: 1525
Fragments arranged by size:
2434 1696 1525 655 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3609 7841 3609
Size: 4232 3614
BlpI GC'TnA_GC
Cuts at: 2249 4326 5263 2249
Size: 2077 937 4832
Fragments arranged by size:
4832 2077 937
Bme1580I G_kGCm'C
Cuts at: 1790 2182 2481 2633 2984 3392 5907 7063 1790
Size: 392 299 152 351 408 2515 1156 2573
Fragments arranged by size:
2573 2515 1156 408 392 351 299 152
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7846
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3144 4899 5577
Size: 416 53 83 399 24 1727 1755 678
Cuts at: 5577 6535 442
Size: 958 1753
Fragments arranged by size:
1755 1753 1727 958 678 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3471 1056
Size: 2415 5431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2714 3473 4496 6506 1239
Size: 1475 759 1023 2010 2579
Fragments arranged by size:
2579 2010 1475 1023 759
Bpu10I CC'TnA_GC
Cuts at: 2111 4196 4314 4566 4865 2111
Size: 2085 118 252 299 5092
Fragments arranged by size:
5092 2085 299 252 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2249 3923 5971 6836 7134 2249
Size: 1674 2048 865 298 2961
Fragments arranged by size:
2961 2048 1674 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3192 6509 882
Size: 617 1693 3317 2219
Fragments arranged by size:
3317 2219 1693 617
BsaAI yAC'GTr
Cuts at: 493 1204 2807 2895 5637 493
Size: 711 1603 88 2742 2702
Fragments arranged by size:
2742 2702 1603 711 88
BsaBI GATnn'nnATC
Cuts at: 7840 7840
Size: 7846
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2223 2350 2562 3756
Size: 53 83 186 936 690 127 212 1194
Cuts at: 3756 4122 5653 6035 6670 275
Size: 366 1531 382 635 1451
Fragments arranged by size:
1531 1451 1194 936 690 635 382 366
212 186 127 83 53
BsaWI w'CCGG_w
Cuts at: 3623 4263 6279 6677 6951 7098 7509 3623
Size: 640 2016 398 274 147 411 3960
Fragments arranged by size:
3960 2016 640 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4542 4572 4938 4968 1178
Size: 30 165 30 3139 30 366 30 4056
Fragments arranged by size:
4056 3139 366 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3288 4999 1186
Size: 2102 1711 4033
Fragments arranged by size:
4033 2102 1711
BseYI C'CCAG_C
Cuts at: 2449 2977 3489 4321 4904 7049 7545 2449
Size: 528 512 832 583 2145 496 2750
Fragments arranged by size:
2750 2145 832 583 528 512 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2618 2814 3555 1163
Size: 573 882 196 741 5454
Fragments arranged by size:
5454 882 741 573 196
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5296 6057 7085 1540
Size: 275 186 3295 761 1028 2301
Fragments arranged by size:
3295 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3978 4637 5907 5992 7063 3978
Size: 659 1270 85 1071 4761
Fragments arranged by size:
4761 1270 1071 659 85
BsmI GAATG_Cn'
Cuts at: 5357 5450 5357
Size: 93 7753
BsmBI CGTCTCn'nnnn_
Cuts at: 4715 4715
Size: 7846
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2182 2481 2633 2956 2984 3298 3392
Size: 399 392 299 152 323 28 314 94
Cuts at: 3392 3978 4315 4637 5165 5907 5992 7063 1391
Size: 586 337 322 528 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 586 528 399 392 337
323 322 314 299 152 94 85 28
BspEI T'CCGG_A
Cuts at: 7509 7509
Size: 7846
BspHI T'CATG_A
Cuts at: 3585 5735 7465 3585
Size: 2150 1730 3966
Fragments arranged by size:
3966 2150 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2036 2202 2295 3682 4636 4685 5201 844
Size: 1192 166 93 1387 954 49 516 3489
Fragments arranged by size:
3489 1387 1192 954 516 166 93 49
BsrBI CCG'CTC
Cuts at: 1394 1823 4330 5122 5733 1394
Size: 429 2507 792 611 3507
Fragments arranged by size:
3507 2507 792 611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6341 6515 65
Size: 1183 5093 174 1396
Fragments arranged by size:
5093 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2535 2817 3623 6490 6677 1700
Size: 110 725 282 806 2867 187 2869
Fragments arranged by size:
2869 2867 806 725 282 187 110
BsrGI T'GTAC_A
Cuts at: 96 4558 96
Size: 4462 3384
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7846
BssSI C'ACGA_G
Cuts at: 3804 5906 6918 3804
Size: 2102 1012 4732
Fragments arranged by size:
4732 2102 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7846
BstBI TT'CG_AA
Cuts at: 3091 6650 3091
Size: 3559 4287
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7846
BstXI CCAn_nnnn'nTGG
Cuts at: 3955 4396 4438 3955
Size: 441 42 7363
Fragments arranged by size:
7363 441 42
BstYI r'GATC_y
Cuts at: 1972 2709 3024 3566 3609 4998 5176 5543 5928
Size: 737 315 542 43 1389 178 367 385
Cuts at: 5928 5945 7397 7483 7495 7841 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1389 737 542 385 367 346
315 178 86 43 17 12
BstZ17I GTA'TAC
Cuts at: 4674 4674
Size: 7846
BtgI C'CryG_G
Cuts at: 513 1065 2004 2132 2187 3192 3595 513
Size: 552 939 128 55 1005 403 4764
Fragments arranged by size:
4764 1005 939 552 403 128 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2986 3909 4318 487
Size: 683 56 1760 923 409 4015
Fragments arranged by size:
4015 1760 923 683 409 56
BtsI GCAGTG_nn'
Cuts at: 2180 4171 4959 5362 6156 6176 2180
Size: 1991 788 403 794 20 3850
Fragments arranged by size:
3850 1991 794 788 403 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7846
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4363 4398 529
Size: 35 3799 35 3977
Fragments arranged by size:
3977 3799 35 35
DraI TTT'AAA
Cuts at: 5150 5150
Size: 7846
DraIII CAC_nnn'GTG
Cuts at: 2608 2608
Size: 7846
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6853 809
Size: 507 5537 1802
Fragments arranged by size:
5537 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2129 3999 4238
Size: 54 1425 50 275 18 299 1870 239
Cuts at: 4238 4250 4331 5293 6182 7811 8
Size: 12 81 962 889 1629 43
Fragments arranged by size:
1870 1629 1425 962 889 299 275 239
81 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 5293 1537
Size: 275 3481 4090
Fragments arranged by size:
4090 3481 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4275 5776 1353
Size: 2922 1501 3423
Fragments arranged by size:
3423 2922 1501
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4832 6433 6807 6953 387
Size: 1064 138 3243 1601 374 146 1280
Fragments arranged by size:
3243 1601 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2702 2714 3060 3473 4496 4515 4579
Size: 393 1070 12 346 413 1023 19 64
Cuts at: 4579 4940 5015 5909 6506 7293 7561 1239
Size: 361 75 894 597 787 268 1524
Fragments arranged by size:
1524 1070 1023 894 787 597 413 393
361 346 268 75 64 19 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2717 2864 3887 2717
Size: 147 1023 6676
Fragments arranged by size:
6676 1023 147
EcoO109I rG'GnC_Cy
Cuts at: 2141 2297 2572 2629 2630 2798 2857 2981 4186
Size: 156 275 57 1 168 59 124 1205
Cuts at: 4186 4318 5129 2141
Size: 132 811 4858
Fragments arranged by size:
4858 1205 811 275 168 156 132 124
59 57 1
EcoRI G'AATT_C
Cuts at: 2927 5155 5533 2927
Size: 2228 378 5240
Fragments arranged by size:
5240 2228 378
EcoRV GAT'ATC
Cuts at: 3122 3122
Size: 7846
FseI GG_CCGG'CC
Cuts at: 2821 2821
Size: 7846
FspI TGC'GCA
Cuts at: 4415 6352 4415
Size: 1937 5909
HaeII r_GCGC'y
Cuts at: 1678 2012 2226 2256 2353 3759 4369 4994 6993
Size: 334 214 30 97 1406 610 625 1999
Cuts at: 6993 1678
Size: 2531
Fragments arranged by size:
2531 1999 1406 625 610 334 214 97
30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2719 2751 3326 3358 6487
Size: 32 135 32 1147 32 575 32 3129
Cuts at: 6487 6519 6561 6593 1373
Size: 32 42 32 2626
Fragments arranged by size:
3129 2626 1147 575 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4775 5190 5436 669
Size: 988 281 2837 415 246 3079
Fragments arranged by size:
3079 2837 988 415 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7846
HpaI GTT'AAC
Cuts at: 5436 5436
Size: 7846
KasI G'GCGC_C
Cuts at: 2222 2349 3755 2222
Size: 127 1406 6313
Fragments arranged by size:
6313 1406 127
KpnI G_GTAC'C
Cuts at: 5171 5171
Size: 7846
MfeI C'AATT_G
Cuts at: 2485 3375 5445 2485
Size: 890 2070 4886
Fragments arranged by size:
4886 2070 890
MluI A'CGCG_T
Cuts at: 7722 7722
Size: 7846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3972 6960 7144 1305
Size: 2667 2988 184 2007
Fragments arranged by size:
2988 2667 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2131 4001 10
Size: 54 1425 343 299 1870 3855
Fragments arranged by size:
3855 1870 1425 343 299 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3531 4106 4640 5804 6163 6322 518
Size: 648 2365 575 534 1164 359 159 2042
Fragments arranged by size:
2365 2042 1164 648 575 534 359 159
MspA1I CmG'CkG
Cuts at: 1606 2147 2248 4237 4325 4864 5262 5939 7087
Size: 541 101 1989 88 539 398 677 1148
Cuts at: 7087 7332 1606
Size: 245 2120
Fragments arranged by size:
2120 1989 1148 677 541 539 398 245
101 88
NaeI GCC'GGC
Cuts at: 2819 2819
Size: 7846
NarI GG'CG_CC
Cuts at: 2223 2350 3756 2223
Size: 127 1406 6313
Fragments arranged by size:
6313 1406 127
NcoI C'CATG_G
Cuts at: 513 1065 2004 3192 3595 513
Size: 552 939 1188 403 4764
Fragments arranged by size:
4764 1188 939 552 403
NdeI CA'TA_TG
Cuts at: 387 3714 387
Size: 3327 4519
NgoMIV G'CCGG_C
Cuts at: 2817 2817
Size: 7846
NheI G'CTAG_C
Cuts at: 1052 3467 1052
Size: 2415 5431
NotI GC'GGCC_GC
Cuts at: 5293 5293
Size: 7846
NspI r_CATG'y
Cuts at: 1132 1229 3475 3662 4111 5204 6749 1132
Size: 97 2246 187 449 1093 1545 2229
Fragments arranged by size:
2246 2229 1545 1093 449 187 97
PasI CC'CwG_GG
Cuts at: 1348 3282 1348
Size: 1934 5912
PciI A'CATG_T
Cuts at: 3658 6745 3658
Size: 3087 4759
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4340 4528 1108
Size: 3232 188 4426
Fragments arranged by size:
4426 3232 188
PfoI T'CCnGG_A
Cuts at: 5134 5134
Size: 7846
PmeI GTTT'AAAC
Cuts at: 5150 5150
Size: 7846
PmlI CAC'GTG
Cuts at: 2807 2807
Size: 7846
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5915 5947 7428 7460 5915
Size: 32 1481 32 6301
Fragments arranged by size:
6301 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2141 2297 4318 5129 2141
Size: 156 2021 811 4858
Fragments arranged by size:
4858 2021 811 156
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7846
PsiI TTA'TAA
Cuts at: 5416 5416
Size: 7846
PspOMI G'GGCC_C
Cuts at: 2629 2980 2629
Size: 351 7495
PstI C_TGCA'G
Cuts at: 830 2580 3229 3504 4149 5198 830
Size: 1750 649 275 645 1049 3478
Fragments arranged by size:
3478 1750 1049 649 645 275
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7846
PvuII CAG'CTG
Cuts at: 4325 4864 4325
Size: 539 7307
SalI G'TCGA_C
Cuts at: 1936 5188 1936
Size: 3252 4594
SanDI GG'GwC_CC
Cuts at: 2141 2141
Size: 7846
SapI GCTCTTCn'nnn_
Cuts at: 4275 4275
Size: 7846
SbfI CC_TGCA'GG
Cuts at: 2580 4149 5198 2580
Size: 1569 1049 5228
Fragments arranged by size:
5228 1569 1049
ScaI AGT'ACT
Cuts at: 6094 7806 6094
Size: 1712 6134
SexAI A'CCwGG_T
Cuts at: 2604 2604
Size: 7846
SfcI C'TryA_G
Cuts at: 826 1045 2576 2811 3225 3500 4126 4145 5194
Size: 219 1531 235 414 275 626 19 1049
Cuts at: 5194 6329 7010 7201 826
Size: 1135 681 191 1471
Fragments arranged by size:
1531 1471 1135 1049 681 626 414 275
235 219 191 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 4761 4761
Size: 7846
SfoI GGC'GCC
Cuts at: 2224 2351 3757 2224
Size: 127 1406 6313
Fragments arranged by size:
6313 1406 127
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7846
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7846
SmaI CCC'GGG
Cuts at: 1872 2065 2843 2957 3077 5173 1872
Size: 193 778 114 120 2096 4545
Fragments arranged by size:
4545 2096 778 193 120 114
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2264 3938 5950 6851 7113 820
Size: 197 926 321 1674 2012 901 262 1553
Fragments arranged by size:
2012 1674 1553 926 901 321 262 197
SnaBI TAC'GTA
Cuts at: 493 5637 493
Size: 5144 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7846
SphI G_CATG'C
Cuts at: 1132 4111 5204 1132
Size: 2979 1093 3774
Fragments arranged by size:
3774 2979 1093
SspI AAT'ATT
Cuts at: 5 52 5770 5
Size: 47 5718 2081
Fragments arranged by size:
5718 2081 47
StuI AGG'CCT
Cuts at: 1517 2870 4469 4571 1517
Size: 1353 1599 102 4792
Fragments arranged by size:
4792 1599 1353 102
StyI C'CwwG_G
Cuts at: 513 1065 2004 3192 3595 5019 5285 513
Size: 552 939 1188 403 1424 266 3074
Fragments arranged by size:
3074 1424 1188 939 552 403 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2441 2660 3180 3274 5873 5890 6043
Size: 1255 861 219 520 94 2599 17 153
Cuts at: 6043 6228 6673 325
Size: 185 445 1498
Fragments arranged by size:
2599 1498 1255 861 520 445 219 185
153 94 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4558 6092 7804
Size: 274 80 33 51 1443 2581 1534 1712
Cuts at: 7804 96
Size: 138
Fragments arranged by size:
2581 1712 1534 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2133 2165 2482 2514 4938 4970 7806 7838 2133
Size: 32 317 32 2424 32 2836 32 2141
Fragments arranged by size:
2836 2424 2141 317 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3179 1316
Size: 1863 5983
XbaI T'CTAG_A
Cuts at: 3826 5182 3826
Size: 1356 6490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3199 3955 3199
Size: 756 7090
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7846
XmaI C'CCGG_G
Cuts at: 1870 2063 2841 2955 3075 5171 1870
Size: 193 778 114 120 2096 4545
Fragments arranged by size:
4545 2096 778 193 120 114
XmnI GAAnn'nnTTC
Cuts at: 5975 5975
Size: 7846
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4123 5654 276
Size: 53 83 186 936 2589 1531 2468
Fragments arranged by size:
2589 2468 1531 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI Bsu36I
EcoICRI FalI FspAI NruI NsiI PacI PspXI PsrI
RsrII SacI SacII SrfI SwaI