Restriction map for
pFN21AE3353
(Circular) (Six-base) MAPSORT of: pFN21AE3353.seq Check: 2709 from: 1 to: 7837
pFN21AE3353 7837 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2225 2719 3844 4203 2225
Size: 494 1125 359 5859
Fragments arranged by size:
5859 1125 494 359
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4401 5647 278
Size: 53 83 186 936 2865 1246 2468
Fragments arranged by size:
2865 2468 1246 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2094 5180 1937
Size: 157 3086 4594
Fragments arranged by size:
4594 3086 157
Acc65I G'GTAC_C
Cuts at: 2546 5158 2546
Size: 2612 5225
AclI AA'CG_TT
Cuts at: 1563 2583 5964 6337 1563
Size: 1020 3381 373 3063
Fragments arranged by size:
3381 3063 1020 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3742 4100 4251 4601 5060 5900 7284 7552
Size: 2110 358 151 350 459 840 1384 268
Cuts at: 7552 1632
Size: 1917
Fragments arranged by size:
2110 1917 1384 840 459 358 350 268
151
AflII C'TTAA_G
Cuts at: 820 1017 3299 820
Size: 197 2282 5358
Fragments arranged by size:
5358 2282 197
AflIII A'CryG_T
Cuts at: 1203 3324 5115 6736 7713 1203
Size: 2121 1791 1621 977 1327
Fragments arranged by size:
2121 1791 1621 1327 977
AgeI A'CCGG_T
Cuts at: 6668 6668
Size: 7837
AhdI GACnn_n'nnGTC
Cuts at: 6566 6566
Size: 7837
AleI CACnn'nnGTG
Cuts at: 1166 4664 1166
Size: 3498 4339
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2816 3077 1184
Size: 1632 261 5944
Fragments arranged by size:
5944 1632 261
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2954 2986 2954
Size: 32 7805
AlwNI CAG_nnn'CTG
Cuts at: 2149 2473 2698 3127 3838 5580 7152 2149
Size: 324 225 429 711 1742 1572 2834
Fragments arranged by size:
2834 1742 1572 711 429 324 225
ApaI G_GGCC'C
Cuts at: 2572 3916 2572
Size: 1344 6493
ApaLI G'TGCA_C
Cuts at: 4249 5894 7050 4249
Size: 1645 1156 5036
Fragments arranged by size:
5036 1645 1156
ApoI r'AATT_y
Cuts at: 1493 2033 3896 4756 4859 5146 5372 5524 7633
Size: 540 1863 860 103 287 226 152 2109
Cuts at: 7633 1493
Size: 1697
Fragments arranged by size:
2109 1863 1697 860 540 287 226 152
103
AseI AT'TA_AT
Cuts at: 160 1032 6391 160
Size: 872 5359 1606
Fragments arranged by size:
5359 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7837
AvaI C'yCGr_G
Cuts at: 1870 1943 2198 3431 4594 5162 1870
Size: 73 255 1233 1163 568 4545
Fragments arranged by size:
4545 1233 1163 568 255 73
BamHI G'GATC_C
Cuts at: 5167 5534 5167
Size: 367 7470
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2154 2546 3951 4908 5158
Size: 325 217 627 367 392 1405 957 250
Cuts at: 5158 6613 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1405 957 627 392 367 325
250 217
BanII G_rGCy'C
Cuts at: 1391 2523 2572 2985 3111 3916 4112 4971 5156
Size: 1132 49 413 126 805 196 859 185
Cuts at: 5156 1391
Size: 4072
Fragments arranged by size:
4072 1132 859 805 413 196 185 126
49
BbeI G_GCGC'C
Cuts at: 4912 4912
Size: 7837
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2721 4124 4847 928
Size: 971 822 1403 723 3918
Fragments arranged by size:
3918 1403 971 822 723
BbvCI CC'TCA_GC
Cuts at: 2129 2129
Size: 7837
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6028 6062 1903
Size: 34 50 34 4007 34 3678
Fragments arranged by size:
4007 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5729 6939 5729
Size: 1210 6627
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7837
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3212 3780 6448
Size: 107 122 71 631 605 1540 568 2668
Cuts at: 6448 136
Size: 1525
Fragments arranged by size:
2668 1540 1525 631 605 568 122 107
71
BglII A'GATC_T
Cuts at: 3474 7832 3474
Size: 4358 3479
BlpI GC'TnA_GC
Cuts at: 4106 4970 5254 4106
Size: 864 284 6689
Fragments arranged by size:
6689 864 284
Bme1580I G_kGCm'C
Cuts at: 1790 2572 2665 3377 3916 4253 5898 7054 1790
Size: 782 93 712 539 337 1645 1156 2573
Fragments arranged by size:
2573 1645 1156 782 712 539 337 93
BmgBI CAC'GTC
Cuts at: 1318 2278 2638 1318
Size: 960 360 6517
Fragments arranged by size:
6517 960 360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2158 5024 5568
Size: 416 53 83 399 24 741 2866 544
Cuts at: 5568 6526 442
Size: 958 1753
Fragments arranged by size:
2866 1753 958 741 544 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3644 1056
Size: 2588 5249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 4204 2407
Size: 1797 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 2537 2981 3149 3488 3569 3764 4028
Size: 1031 267 444 168 339 81 195 264
Cuts at: 4028 6497 1239
Size: 2469 2579
Fragments arranged by size:
2579 2469 1031 444 339 267 264 195
168 81
Bpu10I CC'TnA_GC
Cuts at: 2129 3734 4965 2129
Size: 1605 1231 5001
Fragments arranged by size:
5001 1605 1231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3845 4982 5962 6827 7125 3845
Size: 1137 980 865 298 4557
Fragments arranged by size:
4557 1137 980 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6500 882
Size: 617 5001 2219
Fragments arranged by size:
5001 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3778 3973 5628 493
Size: 711 2574 195 1655 2702
Fragments arranged by size:
2702 2574 1655 711 195
BsaBI GATnn'nnATC
Cuts at: 7831 7831
Size: 7837
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2266 4398 4909 5644
Size: 53 83 186 936 733 2132 511 735
Cuts at: 5644 6026 6661 275
Size: 382 635 1451
Fragments arranged by size:
2132 1451 936 735 733 635 511 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 6270 6668 6942 7089 7500 6270
Size: 398 274 147 411 6607
Fragments arranged by size:
6607 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2954 2984 3098 3128 3767
Size: 30 165 30 1551 30 114 30 639
Cuts at: 3767 3797 3944 3974 1178
Size: 30 147 30 5041
Fragments arranged by size:
5041 1551 639 165 147 114 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2351 2462 2618 2833 3533 3536 4316
Size: 877 288 111 156 215 700 3 780
Cuts at: 4316 4637 5042 5045 1186
Size: 321 405 3 3978
Fragments arranged by size:
3978 877 780 700 405 321 288 215
156 111 3 3
BseYI C'CCAG_C
Cuts at: 2473 2478 3484 4214 4669 7040 7536 2473
Size: 5 1006 730 455 2371 496 2774
Fragments arranged by size:
2774 2371 1006 730 496 455 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 2786 3053 3872 1163
Size: 573 564 486 267 819 5128
Fragments arranged by size:
5128 819 573 564 486 267
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2326 2457 4684 5287 6048 7076
Size: 275 186 325 131 2227 603 761 1028
Cuts at: 7076 1540
Size: 2301
Fragments arranged by size:
2301 2227 1028 761 603 325 275 186
131
BsiHKAI G_wGCw'C
Cuts at: 2985 3104 3582 3627 3713 3861 4112 4253 4971
Size: 119 478 45 86 148 251 141 718
Cuts at: 4971 5898 5983 7054 2985
Size: 927 85 1071 3768
Fragments arranged by size:
3768 1071 927 718 478 251 148 141
119 86 85 45
BsmI GAATG_Cn'
Cuts at: 5348 5441 5348
Size: 93 7744
BsmBI CGTCTCn'nnnn_
Cuts at: 3176 3176
Size: 7837
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2523 2572 2665 2985 3104 3111 3377
Size: 399 733 49 93 320 119 7 266
Cuts at: 3377 3582 3627 3713 3861 3916 4112 4253 4971
Size: 205 45 86 148 55 196 141 718
Cuts at: 4971 5156 5898 5983 7054 1391
Size: 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 733 718 399 320 266
205 196 185 148 141 119 93 86
85 55 49 45 7
BspEI T'CCGG_A
Cuts at: 7500 7500
Size: 7837
BspHI T'CATG_A
Cuts at: 4701 5726 7456 4701
Size: 1025 1730 5082
Fragments arranged by size:
5082 1730 1025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2225 2719 3162 3711 3844 3846 4203 5125
Size: 1381 494 443 549 133 2 357 922
Cuts at: 5125 5192 844
Size: 67 3489
Fragments arranged by size:
3489 1381 922 549 494 443 357 133
67 2
BsrBI CCG'CTC
Cuts at: 1394 1823 2176 2770 3016 3553 3960 5724 1394
Size: 429 353 594 246 537 407 1764 3507
Fragments arranged by size:
3507 1764 594 537 429 407 353 246
BsrDI GCAATG_nn'
Cuts at: 65 1248 2125 3255 6332 6506 65
Size: 1183 877 1130 3077 174 1396
Fragments arranged by size:
3077 1396 1183 1130 877 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2463 6481 6668 1700
Size: 110 653 4018 187 2869
Fragments arranged by size:
4018 2869 653 187 110
BsrGI T'GTAC_A
Cuts at: 96 4290 96
Size: 4194 3643
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7837
BssSI C'ACGA_G
Cuts at: 2381 3581 5897 6909 2381
Size: 1200 2316 1012 3309
Fragments arranged by size:
3309 2316 1200 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3850 4366 794
Size: 3056 516 4265
Fragments arranged by size:
4265 3056 516
BstBI TT'CG_AA
Cuts at: 6641 6641
Size: 7837
BstEII G'GTnAC_C
Cuts at: 1184 3658 1184
Size: 2474 5363
BstYI r'GATC_y
Cuts at: 1972 3474 5167 5534 5919 5936 7388 7474 7486
Size: 1502 1693 367 385 17 1452 86 12
Cuts at: 7486 7832 1972
Size: 346 1977
Fragments arranged by size:
1977 1693 1502 1452 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4427 4427
Size: 7837
BtgI C'CryG_G
Cuts at: 513 1065 2028 2829 3049 3369 4912 513
Size: 552 963 801 220 320 1543 3438
Fragments arranged by size:
3438 1543 963 801 552 320 220
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4489 487
Size: 683 56 3263 3835
Fragments arranged by size:
3835 3263 683 56
BtsI GCAGTG_nn'
Cuts at: 4253 5353 6147 6167 4253
Size: 1100 794 20 5923
Fragments arranged by size:
5923 1100 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7837
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7802
DraI TTT'AAA
Cuts at: 5141 5141
Size: 7837
DraIII CAC_nnn'GTG
Cuts at: 3451 3666 5116 3451
Size: 215 1450 6172
Fragments arranged by size:
6172 1450 215
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6844 809
Size: 507 5528 1802
Fragments arranged by size:
5528 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2454 3716 3918
Size: 54 1425 50 275 18 624 1262 202
Cuts at: 3918 3934 4656 4741 5284 6173 7802 8
Size: 16 722 85 543 889 1629 43
Fragments arranged by size:
1629 1425 1262 889 722 624 543 275
202 85 54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2454 5284 1537
Size: 275 642 2830 4090
Fragments arranged by size:
4090 2830 642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2183 4928 5767 1353
Size: 830 2745 839 3423
Fragments arranged by size:
3423 2745 839 830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3260 6424 6798 6944 387
Size: 1064 138 1671 3164 374 146 1280
Fragments arranged by size:
3164 1671 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2983 4110 4969 2983
Size: 1127 859 5851
Fragments arranged by size:
5851 1127 859
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2537 2981 3149 3488 3569 3742
Size: 393 638 267 444 168 339 81 173
Cuts at: 3742 3764 4028 4100 4251 4601 5060 5900 6497
Size: 22 264 72 151 350 459 840 597
Cuts at: 6497 7284 7552 1239
Size: 787 268 1524
Fragments arranged by size:
1524 840 787 638 597 459 444 393
350 339 268 267 264 173 168 151
81 72 22
EcoNI CCTnn'n_nnAGG
Cuts at: 3431 4018 4358 4478 3431
Size: 587 340 120 6790
Fragments arranged by size:
6790 587 340 120
EcoO109I rG'GnC_Cy
Cuts at: 2540 2569 3589 3913 4084 4183 4603 4890 2540
Size: 29 1020 324 171 99 420 287 5487
Fragments arranged by size:
5487 1020 420 324 287 171 99 29
EcoRI G'AATT_C
Cuts at: 2033 5146 5524 2033
Size: 3113 378 4346
Fragments arranged by size:
4346 3113 378
FspI TGC'GCA
Cuts at: 3383 6343 3383
Size: 2960 4877
HaeII r_GCGC'y
Cuts at: 1678 2651 3408 3641 3741 4912 6984 1678
Size: 973 757 233 100 1171 2072 2531
Fragments arranged by size:
2531 2072 1171 973 757 233 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 6478 6510 6552 6584 1373
Size: 32 135 32 4906 32 42 32 2626
Fragments arranged by size:
4906 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2071 2581 2814 5181 5427 669
Size: 988 281 133 510 233 2367 246 3079
Fragments arranged by size:
3079 2367 988 510 281 246 233 133
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7837
HpaI GTT'AAC
Cuts at: 5427 5427
Size: 7837
KasI G'GCGC_C
Cuts at: 4908 4908
Size: 7837
KpnI G_GTAC'C
Cuts at: 2550 5162 2550
Size: 2612 5225
MfeI C'AATT_G
Cuts at: 5436 5436
Size: 7837
MluI A'CGCG_T
Cuts at: 7713 7713
Size: 7837
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4540 6951 7135 1305
Size: 3235 2411 184 2007
Fragments arranged by size:
3235 2411 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3920 4658 4743 10
Size: 54 1425 343 2088 738 85 3104
Fragments arranged by size:
3104 2088 1425 738 343 85 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3681 4043 4256 4481 4664 5795 6154
Size: 648 2515 362 213 225 183 1131 359
Cuts at: 6154 6313 518
Size: 159 2042
Fragments arranged by size:
2515 2042 1131 648 362 359 225 213
183 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2234 2473 2515 2644 2698 2796 3124
Size: 540 88 239 42 129 54 98 328
Cuts at: 3124 3410 3529 3535 3847 4314 4723 4974 5253
Size: 286 119 6 312 467 409 251 279
Cuts at: 5253 5930 7078 7323 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 540 467 409 328 312
286 279 251 245 239 129 119 98
88 54 42 6
NaeI GCC'GGC
Cuts at: 2465 2465
Size: 7837
NarI GG'CG_CC
Cuts at: 4909 4909
Size: 7837
NcoI C'CATG_G
Cuts at: 513 1065 2028 2829 513
Size: 552 963 801 5521
Fragments arranged by size:
5521 963 801 552
NdeI CA'TA_TG
Cuts at: 387 4663 387
Size: 4276 3561
NgoMIV G'CCGG_C
Cuts at: 2463 2463
Size: 7837
NheI G'CTAG_C
Cuts at: 1052 3640 1052
Size: 2588 5249
NotI GC'GGCC_GC
Cuts at: 2454 5284 2454
Size: 2830 5007
NspI r_CATG'y
Cuts at: 1132 1229 2188 3328 3512 4162 4369 5195 6740
Size: 97 959 1140 184 650 207 826 1545
Cuts at: 6740 1132
Size: 2229
Fragments arranged by size:
2229 1545 1140 959 826 650 207 184
97
PasI CC'CwG_GG
Cuts at: 1348 2012 1348
Size: 664 7173
PciI A'CATG_T
Cuts at: 3324 6736 3324
Size: 3412 4425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3261 4393 4750 1108
Size: 2153 1132 357 4195
Fragments arranged by size:
4195 2153 1132 357
PfoI T'CCnGG_A
Cuts at: 3546 3618 3630 4005 3546
Size: 72 12 375 7378
Fragments arranged by size:
7378 375 72 12
PmeI GTTT'AAAC
Cuts at: 5141 5141
Size: 7837
PmlI CAC'GTG
Cuts at: 3973 3973
Size: 7837
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2954 2986 3303 3335 5906 5938 7419 7451 2954
Size: 32 317 32 2571 32 1481 32 3340
Fragments arranged by size:
3340 2571 1481 317 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3589 3589
Size: 7837
PshAI GACnn'nnGTC
Cuts at: 1613 2831 1613
Size: 1218 6619
PsiI TTA'TAA
Cuts at: 5407 5407
Size: 7837
PspOMI G'GGCC_C
Cuts at: 2568 3912 2568
Size: 1344 6493
PstI C_TGCA'G
Cuts at: 830 2496 2514 2730 2795 3534 3846 4022 4200
Size: 1666 18 216 65 739 312 176 178
Cuts at: 4200 4380 5189 830
Size: 180 809 3478
Fragments arranged by size:
3478 1666 809 739 312 216 180 178
176 65 18
PvuI CG_AT'CG
Cuts at: 2001 4684 2001
Size: 2683 5154
PvuII CAG'CTG
Cuts at: 2146 2473 2515 2796 3529 3535 3847 4723 4974
Size: 327 42 281 733 6 312 876 251
Cuts at: 4974 2146
Size: 5009
Fragments arranged by size:
5009 876 733 327 312 281 251 42
6
SacI G_AGCT'C
Cuts at: 2985 4112 4971 2985
Size: 1127 859 5851
Fragments arranged by size:
5851 1127 859
SalI G'TCGA_C
Cuts at: 1936 5179 1936
Size: 3243 4594
SapI GCTCTTCn'nnn_
Cuts at: 2183 2183
Size: 7837
SbfI CC_TGCA'GG
Cuts at: 5189 5189
Size: 7837
ScaI AGT'ACT
Cuts at: 6085 7797 6085
Size: 1712 6125
SexAI A'CCwGG_T
Cuts at: 3591 3591
Size: 7837
SfcI C'TryA_G
Cuts at: 826 1045 2492 2510 2726 2791 3530 3842 4018
Size: 219 1447 18 216 65 739 312 176
Cuts at: 4018 4196 4376 5185 6320 7001 7192 826
Size: 178 180 809 1135 681 191 1471
Fragments arranged by size:
1471 1447 1135 809 739 681 312 219
216 191 180 178 176 65 18
SfoI GGC'GCC
Cuts at: 4910 4910
Size: 7837
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7837
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7837
SmaI CCC'GGG
Cuts at: 1872 4596 5164 1872
Size: 2724 568 4545
Fragments arranged by size:
4545 2724 568
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3299 3860 4997 5941 6842 7104
Size: 197 926 1356 561 1137 944 901 262
Cuts at: 7104 820
Size: 1553
Fragments arranged by size:
1553 1356 1137 944 926 901 561 262
197
SnaBI TAC'GTA
Cuts at: 493 5628 493
Size: 5135 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7837
SphI G_CATG'C
Cuts at: 1132 4162 5195 1132
Size: 3030 1033 3774
Fragments arranged by size:
3774 3030 1033
SspI AAT'ATT
Cuts at: 5 52 5761 5
Size: 47 5709 2081
Fragments arranged by size:
5709 2081 47
StuI AGG'CCT
Cuts at: 1517 2204 2376 2486 4013 4172 4473 4959 1517
Size: 687 172 110 1527 159 301 486 4395
Fragments arranged by size:
4395 1527 687 486 301 172 159 110
StyI C'CwwG_G
Cuts at: 513 1065 2028 2043 2631 2829 4137 5276 513
Size: 552 963 15 588 198 1308 1139 3074
Fragments arranged by size:
3074 1308 1139 963 588 552 198 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4046 5864 5881 6034 6219 6664 325
Size: 1255 2466 1818 17 153 185 445 1498
Fragments arranged by size:
2466 1818 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 2784 3453
Size: 274 80 33 51 1443 519 288 669
Cuts at: 3453 4290 6083 7795 96
Size: 837 1793 1712 138
Fragments arranged by size:
1793 1712 1443 837 669 519 288 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3098 3130 3581 3613 3767 3799 3944 3976 4184
Size: 32 451 32 154 32 145 32 208
Cuts at: 4184 4216 4388 4420 5102 5134 7797 7829 3098
Size: 32 172 32 682 32 2663 32 3106
Fragments arranged by size:
3106 2663 682 451 208 172 154 145
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3076 4097 4817 1316
Size: 1760 1021 720 4336
Fragments arranged by size:
4336 1760 1021 720
XbaI T'CTAG_A
Cuts at: 5173 5173
Size: 7837
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2189 3901 4222 4666 4736 2189
Size: 1712 321 444 70 5290
Fragments arranged by size:
5290 1712 444 321 70
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7837
XmaI C'CCGG_G
Cuts at: 1870 4594 5162 1870
Size: 2724 568 4545
Fragments arranged by size:
4545 2724 568
XmnI GAAnn'nnTTC
Cuts at: 4094 5045 5966 4094
Size: 951 921 5965
Fragments arranged by size:
5965 951 921
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4399 5645 276
Size: 53 83 186 936 2865 1246 2468
Fragments arranged by size:
2865 2468 1246 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I
EcoRV FalI FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SacII SanDI SfiI SrfI SwaI