(Circular) (Six-base) MAPSORT of: pFN21AE3344.seq Check: 1648 from: 1 to: 7717 pFN21AE3344 7717 bp With 182 enzymes: SgfI * August 14, 2013 14:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3157 4081 3157 Size: 924 6793 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2301 4719 5527 278 Size: 53 83 186 936 765 2418 808 2468 Fragments arranged by size: 2468 2418 936 808 765 186 83 53 AccI GT'mk_AC Cuts at: 1937 2301 5060 1937 Size: 364 2759 4594 Fragments arranged by size: 4594 2759 364 Acc65I G'GTAC_C Cuts at: 5038 5038 Size: 7717 AclI AA'CG_TT Cuts at: 1563 5844 6217 1563 Size: 4281 373 3063 Fragments arranged by size: 4281 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2255 2300 2323 2606 2711 2825 5780 7164 Size: 623 45 23 283 105 114 2955 1384 Cuts at: 7164 7432 1632 Size: 268 1917 Fragments arranged by size: 2955 1917 1384 623 283 268 114 105 45 23 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7520 AflIII A'CryG_T Cuts at: 1203 6616 7593 1203 Size: 5413 977 1327 Fragments arranged by size: 5413 1327 977 AgeI A'CCGG_T Cuts at: 6548 6548 Size: 7717 AhdI GACnn_n'nnGTC Cuts at: 2776 6446 2776 Size: 3670 4047 AleI CACnn'nnGTG Cuts at: 1166 4442 1166 Size: 3276 4441 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2645 1184 Size: 1461 6256 AlwNI CAG_nnn'CTG Cuts at: 2470 2701 2755 2875 2905 2992 3139 3295 3336 Size: 231 54 120 30 87 147 156 41 Cuts at: 3336 3454 3507 4006 4312 4734 4852 5460 7032 Size: 118 53 499 306 422 118 608 1572 Cuts at: 7032 2470 Size: 3155 Fragments arranged by size: 3155 1572 608 499 422 306 231 156 147 120 118 118 87 54 53 41 30 ApaLI G'TGCA_C Cuts at: 4270 5774 6930 4270 Size: 1504 1156 5057 Fragments arranged by size: 5057 1504 1156 ApoI r'AATT_y Cuts at: 1493 5026 5252 5404 7513 1493 Size: 3533 226 152 2109 1697 Fragments arranged by size: 3533 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6271 160 Size: 872 5239 1606 Fragments arranged by size: 5239 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7717 AvaI C'yCGr_G Cuts at: 1870 1943 2241 3532 3802 3835 4027 5013 5042 Size: 73 298 1291 270 33 192 986 29 Cuts at: 5042 1870 Size: 4545 Fragments arranged by size: 4545 1291 986 298 270 192 73 33 29 BamHI G'GATC_C Cuts at: 3696 5047 5414 3696 Size: 1351 367 5999 Fragments arranged by size: 5999 1351 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2460 3839 4202 4444 4832 Size: 325 217 627 673 1379 363 242 388 Cuts at: 4832 4891 5038 6493 618 Size: 59 147 1455 1842 Fragments arranged by size: 1842 1455 1379 673 627 388 363 325 242 217 147 59 BanII G_rGCy'C Cuts at: 1391 3273 3541 3585 3809 3879 4228 4406 5012 Size: 1882 268 44 224 70 349 178 606 Cuts at: 5012 5036 1391 Size: 24 4072 Fragments arranged by size: 4072 1882 606 349 268 224 178 70 44 24 BbeI G_GCGC'C Cuts at: 4895 4895 Size: 7717 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2243 928 Size: 971 344 6402 Fragments arranged by size: 6402 971 344 BbvCI CC'TCA_GC Cuts at: 2579 2579 Size: 7717 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2483 2517 5908 5942 1903 Size: 34 546 34 3391 34 3678 Fragments arranged by size: 3678 3391 546 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4180 5609 6819 4180 Size: 1429 1210 5078 Fragments arranged by size: 5078 1429 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7717 BfrBI ATG'CAT Cuts at: 3307 3737 3307 Size: 430 7287 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2210 4019 6328 Size: 107 122 71 631 605 538 1809 2309 Cuts at: 6328 136 Size: 1525 Fragments arranged by size: 2309 1809 1525 631 605 538 122 107 71 BglII A'GATC_T Cuts at: 7712 7712 Size: 7717 BlpI GC'TnA_GC Cuts at: 2600 4227 4949 5134 2600 Size: 1627 722 185 5183 Fragments arranged by size: 5183 1627 722 185 Bme1580I G_kGCm'C Cuts at: 1790 2220 3842 4274 4449 4506 4835 5778 6934 Size: 430 1622 432 175 57 329 943 1156 Cuts at: 6934 1790 Size: 2573 Fragments arranged by size: 2573 1622 1156 943 432 430 329 175 57 BmgBI CAC'GTC Cuts at: 1318 3390 4117 4777 1318 Size: 2072 727 660 4258 Fragments arranged by size: 4258 2072 727 660 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2980 5448 6406 Size: 416 53 83 399 24 1563 2468 958 Cuts at: 6406 442 Size: 1753 Fragments arranged by size: 2468 1753 1563 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7717 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3169 3169 Size: 7717 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 2543 2885 3406 3533 3764 3917 3932 Size: 1052 252 342 521 127 231 153 15 Cuts at: 3932 3983 4028 4204 4390 4624 4874 4948 6377 Size: 51 45 176 186 234 250 74 1429 Cuts at: 6377 1239 Size: 2579 Fragments arranged by size: 2579 1429 1052 521 342 252 250 234 231 186 176 153 127 74 51 45 15 Bpu10I CC'TnA_GC Cuts at: 2579 3552 3701 4526 2579 Size: 973 149 825 5770 Fragments arranged by size: 5770 973 825 149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3230 4600 4793 5842 6707 7005 3230 Size: 1370 193 1049 865 298 3942 Fragments arranged by size: 3942 1370 1049 865 298 193 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2360 2489 3081 3522 4872 6380 882 Size: 617 861 129 592 441 1350 1508 2219 Fragments arranged by size: 2219 1508 1350 861 617 592 441 129 BsaAI yAC'GTr Cuts at: 493 1204 4413 5508 493 Size: 711 3209 1095 2702 Fragments arranged by size: 3209 2702 1095 711 BsaBI GATnn'nnATC Cuts at: 4754 7711 4754 Size: 2957 4760 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2298 3666 4716 4892 Size: 53 83 186 936 765 1368 1050 176 Cuts at: 4892 5524 5906 6541 275 Size: 632 382 635 1451 Fragments arranged by size: 1451 1368 1050 936 765 635 632 382 186 176 83 53 BsaWI w'CCGG_w Cuts at: 6150 6548 6822 6969 7380 6150 Size: 398 274 147 411 6487 Fragments arranged by size: 6487 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2045 2075 2666 2696 4527 Size: 30 165 30 642 30 591 30 1831 Cuts at: 4527 4557 1178 Size: 30 4338 Fragments arranged by size: 4338 1831 642 591 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2180 2183 2429 2690 2804 2825 3929 Size: 880 114 3 246 261 114 21 1104 Cuts at: 3929 4487 4535 4604 4856 4886 1186 Size: 558 48 69 252 30 4017 Fragments arranged by size: 4017 1104 880 558 261 252 246 114 114 69 48 30 21 3 BseYI C'CCAG_C Cuts at: 2111 2390 3440 4388 4656 6920 7416 2111 Size: 279 1050 948 268 2264 496 2412 Fragments arranged by size: 2412 2264 1050 948 496 279 268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2162 2498 2597 4097 4720 4727 1163 Size: 573 426 336 99 1500 623 7 4153 Fragments arranged by size: 4153 1500 623 573 426 336 99 7 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5167 5928 6956 1540 Size: 275 186 3166 761 1028 2301 Fragments arranged by size: 3166 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2481 3118 3246 3585 4274 4497 5778 5863 6934 Size: 637 128 339 689 223 1281 85 1071 Cuts at: 6934 2481 Size: 3264 Fragments arranged by size: 3264 1281 1071 689 637 339 223 128 85 BsiWI C'GTAC_G Cuts at: 2095 2095 Size: 7717 BsmI GAATG_Cn' Cuts at: 3052 5228 5321 3052 Size: 2176 93 5448 Fragments arranged by size: 5448 2176 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3318 4783 3318 Size: 1465 6252 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 2481 3118 3246 3273 3541 3585 Size: 399 430 261 637 128 27 268 44 Cuts at: 3585 3809 3842 3879 4228 4274 4406 4449 4497 Size: 224 33 37 349 46 132 43 48 Cuts at: 4497 4506 4835 5012 5036 5778 5863 6934 1391 Size: 9 329 177 24 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 637 430 399 349 329 268 261 224 177 132 128 85 48 46 44 43 37 33 27 24 9 BspEI T'CCGG_A Cuts at: 7380 7380 Size: 7717 BspHI T'CATG_A Cuts at: 5606 7336 5606 Size: 1730 5987 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 3157 4081 5072 844 Size: 1295 1018 924 991 3489 Fragments arranged by size: 3489 1295 1018 991 924 BsrBI CCG'CTC Cuts at: 1394 1823 2935 3420 3663 5604 1394 Size: 429 1112 485 243 1941 3507 Fragments arranged by size: 3507 1941 1112 485 429 243 BsrDI GCAATG_nn' Cuts at: 65 1248 2629 3737 4096 6212 6386 65 Size: 1183 1381 1108 359 2116 174 1396 Fragments arranged by size: 2116 1396 1381 1183 1108 359 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6361 6548 1700 Size: 110 4551 187 2869 Fragments arranged by size: 4551 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3456 96 Size: 3360 4357 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7717 BssSI C'ACGA_G Cuts at: 4499 5777 6789 4499 Size: 1278 1012 5427 Fragments arranged by size: 5427 1278 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2701 794 Size: 1907 5810 BstBI TT'CG_AA Cuts at: 6521 6521 Size: 7717 BstEII G'GTnAC_C Cuts at: 1184 2311 3709 3796 4660 1184 Size: 1127 1398 87 864 4241 Fragments arranged by size: 4241 1398 1127 864 87 BstXI CCAn_nnnn'nTGG Cuts at: 4396 4396 Size: 7717 BstYI r'GATC_y Cuts at: 1972 3516 3696 4521 5047 5414 5799 5816 7268 Size: 1544 180 825 526 367 385 17 1452 Cuts at: 7268 7354 7366 7712 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1544 1452 825 526 385 367 346 180 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2756 2756 Size: 7717 BtgI C'CryG_G Cuts at: 513 1065 2004 2940 3278 3921 4062 513 Size: 552 939 936 338 643 141 4168 Fragments arranged by size: 4168 939 936 643 552 338 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2245 4954 487 Size: 683 56 1019 2709 3250 Fragments arranged by size: 3250 2709 1019 683 56 BtsI GCAGTG_nn' Cuts at: 4274 5233 6027 6047 4274 Size: 959 794 20 5944 Fragments arranged by size: 5944 959 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7717 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7682 DraI TTT'AAA Cuts at: 5021 5021 Size: 7717 DraIII CAC_nnn'GTG Cuts at: 4165 4165 Size: 7717 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6724 809 Size: 507 5408 1802 Fragments arranged by size: 5408 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2490 2646 3468 Size: 54 1425 50 275 18 660 156 822 Cuts at: 3468 4059 5164 6053 7682 8 Size: 591 1105 889 1629 43 Fragments arranged by size: 1629 1425 1105 889 822 660 591 275 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5164 1537 Size: 275 3352 4090 Fragments arranged by size: 4090 3352 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2970 3002 5647 1353 Size: 1617 32 2645 3423 Fragments arranged by size: 3423 2645 1617 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2864 3694 3830 4949 6304 6678 Size: 1064 138 1275 830 136 1119 1355 374 Cuts at: 6678 6824 387 Size: 146 1280 Fragments arranged by size: 1355 1280 1275 1119 1064 830 374 146 138 136 EcoICRI GAG'CTC Cuts at: 3583 3583 Size: 7717 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2291 2300 2323 2543 2606 2711 Size: 393 623 36 9 23 220 63 105 Cuts at: 2711 2825 2885 3406 3533 3764 3917 3932 3983 Size: 114 60 521 127 231 153 15 51 Cuts at: 3983 4028 4204 4390 4624 4874 4948 5780 6377 Size: 45 176 186 234 250 74 832 597 Cuts at: 6377 7164 7432 1239 Size: 787 268 1524 Fragments arranged by size: 1524 832 787 623 597 521 393 268 250 234 231 220 186 176 153 127 114 105 74 63 60 51 45 36 23 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 2135 2411 2672 2807 2879 3704 3914 4250 4369 Size: 276 261 135 72 825 210 336 119 Cuts at: 4369 2135 Size: 5483 Fragments arranged by size: 5483 825 336 276 261 210 135 119 72 EcoO109I rG'GnC_Cy Cuts at: 2509 4003 4108 4816 2509 Size: 1494 105 708 5410 Fragments arranged by size: 5410 1494 708 105 EcoRI G'AATT_C Cuts at: 5026 5404 5026 Size: 378 7339 FspI TGC'GCA Cuts at: 3203 6223 3203 Size: 3020 4697 HaeII r_GCGC'y Cuts at: 1678 2205 4895 6864 1678 Size: 527 2690 1969 2531 Fragments arranged by size: 2690 2531 1969 527 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2762 2794 4605 4637 6358 Size: 32 135 32 1190 32 1811 32 1721 Cuts at: 6358 6390 6432 6464 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1811 1721 1190 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2335 3973 4054 4786 5061 5307 Size: 988 281 397 1638 81 732 275 246 Cuts at: 5307 669 Size: 3079 Fragments arranged by size: 3079 1638 988 732 397 281 275 246 81 HindIII A'AGCT_T Cuts at: 748 748 Size: 7717 HpaI GTT'AAC Cuts at: 5307 5307 Size: 7717 KasI G'GCGC_C Cuts at: 4891 4891 Size: 7717 KpnI G_GTAC'C Cuts at: 5042 5042 Size: 7717 MfeI C'AATT_G Cuts at: 5316 5316 Size: 7717 MluI A'CGCG_T Cuts at: 7593 7593 Size: 7717 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3354 4362 6831 7015 1305 Size: 2049 1008 2469 184 2007 Fragments arranged by size: 2469 2049 2007 1008 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4061 10 Size: 54 1425 343 2229 3666 Fragments arranged by size: 3666 2229 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2568 4442 5675 6034 6193 518 Size: 648 1402 1874 1233 359 159 2042 Fragments arranged by size: 2042 1874 1402 1233 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2158 2470 2521 3220 3292 3507 3610 4653 Size: 552 312 51 699 72 215 103 1043 Cuts at: 4653 4723 4825 4953 5133 5810 6958 7203 1606 Size: 70 102 128 180 677 1148 245 2120 Fragments arranged by size: 2120 1148 1043 699 677 552 312 245 215 180 128 103 102 72 70 51 NarI GG'CG_CC Cuts at: 4892 4892 Size: 7717 NcoI C'CATG_G Cuts at: 513 1065 2004 4062 513 Size: 552 939 2058 4168 Fragments arranged by size: 4168 2058 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7717 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7717 NotI GC'GGCC_GC Cuts at: 5164 5164 Size: 7717 NsiI A_TGCA'T Cuts at: 3309 3739 3309 Size: 430 7287 NspI r_CATG'y Cuts at: 1132 1229 3311 4157 5075 6620 1132 Size: 97 2082 846 918 1545 2229 Fragments arranged by size: 2229 2082 1545 918 846 97 PasI CC'CwG_GG Cuts at: 1348 4506 1348 Size: 3158 4559 PciI A'CATG_T Cuts at: 6616 6616 Size: 7717 PflMI CCAn_nnn'nTGG Cuts at: 1108 3139 4006 4295 4675 1108 Size: 2031 867 289 380 4150 Fragments arranged by size: 4150 2031 867 380 289 PfoI T'CCnGG_A Cuts at: 2268 3519 3894 3999 4251 4626 4710 4851 2268 Size: 1251 375 105 252 375 84 141 5134 Fragments arranged by size: 5134 1251 375 375 252 141 105 84 PmeI GTTT'AAAC Cuts at: 5021 5021 Size: 7717 PmlI CAC'GTG Cuts at: 4413 4413 Size: 7717 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3898 5786 5818 7299 7331 3866 Size: 32 1888 32 1481 32 4252 Fragments arranged by size: 4252 1888 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4003 4108 4003 Size: 105 7612 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7717 PsiI TTA'TAA Cuts at: 3987 5287 3987 Size: 1300 6417 PspXI vC'TCGA_Gb Cuts at: 2241 2241 Size: 7717 PstI C_TGCA'G Cuts at: 830 2325 2412 2475 2634 2673 2706 3000 3057 Size: 1495 87 63 159 39 33 294 57 Cuts at: 3057 3078 3225 3341 3627 4092 5069 830 Size: 21 147 116 286 465 977 3478 Fragments arranged by size: 3478 1495 977 465 294 286 159 147 116 87 63 57 39 33 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7717 PvuII CAG'CTG Cuts at: 2158 2470 2521 3220 3507 3610 4723 4953 2158 Size: 312 51 699 287 103 1113 230 4922 Fragments arranged by size: 4922 1113 699 312 287 230 103 51 SacI G_AGCT'C Cuts at: 3585 3585 Size: 7717 SalI G'TCGA_C Cuts at: 1936 5059 1936 Size: 3123 4594 SbfI CC_TGCA'GG Cuts at: 2412 2673 5069 2412 Size: 261 2396 5060 Fragments arranged by size: 5060 2396 261 ScaI AGT'ACT Cuts at: 2969 4577 5965 7677 2969 Size: 1608 1388 1712 3009 Fragments arranged by size: 3009 1712 1608 1388 SexAI A'CCwGG_T Cuts at: 3186 3186 Size: 7717 SfcI C'TryA_G Cuts at: 826 1045 2321 2408 2471 2617 2630 2669 2702 Size: 219 1276 87 63 146 13 39 33 Cuts at: 2702 2996 3031 3053 3074 3221 3337 3623 4088 Size: 294 35 22 21 147 116 286 465 Cuts at: 4088 4820 5065 6200 6881 7072 826 Size: 732 245 1135 681 191 1471 Fragments arranged by size: 1471 1276 1135 732 681 465 294 286 245 219 191 147 146 116 87 63 39 35 33 22 21 13 SfoI GGC'GCC Cuts at: 4893 4893 Size: 7717 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7717 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7717 SmaI CCC'GGG Cuts at: 1872 3534 3804 3837 5044 1872 Size: 1662 270 33 1207 4545 Fragments arranged by size: 4545 1662 1207 270 33 SmlI C'TyrA_G Cuts at: 820 1017 1943 2241 3245 4579 4808 5821 6722 Size: 197 926 298 1004 1334 229 1013 901 Cuts at: 6722 6984 820 Size: 262 1553 Fragments arranged by size: 1553 1334 1013 1004 926 901 298 262 229 197 SnaBI TAC'GTA Cuts at: 493 5508 493 Size: 5015 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7717 SphI G_CATG'C Cuts at: 1132 3311 4157 5075 1132 Size: 2179 846 918 3774 Fragments arranged by size: 3774 2179 918 846 SrfI GCCC'GGGC Cuts at: 3837 3837 Size: 7717 SspI AAT'ATT Cuts at: 5 52 5641 5 Size: 47 5589 2081 Fragments arranged by size: 5589 2081 47 StuI AGG'CCT Cuts at: 1517 4601 1517 Size: 3084 4633 StyI C'CwwG_G Cuts at: 513 1065 2004 2142 4062 4341 4549 5156 513 Size: 552 939 138 1920 279 208 607 3074 Fragments arranged by size: 3074 1920 939 607 552 279 208 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2393 3857 4648 4715 5744 5761 5914 Size: 1255 813 1464 791 67 1029 17 153 Cuts at: 5914 6099 6544 325 Size: 185 445 1498 Fragments arranged by size: 1498 1464 1255 1029 813 791 445 185 153 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2967 3456 4575 Size: 274 80 33 51 1443 990 489 1119 Cuts at: 4575 5963 7675 96 Size: 1388 1712 138 Fragments arranged by size: 1712 1443 1388 1119 990 489 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2045 2077 7677 7709 2045 Size: 32 5600 32 2053 Fragments arranged by size: 5600 2053 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3188 4730 1316 Size: 1872 1542 4303 Fragments arranged by size: 4303 1872 1542 XbaI T'CTAG_A Cuts at: 5053 5053 Size: 7717 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2266 3400 2266 Size: 1134 6583 XhoI C'TCGA_G Cuts at: 1943 2241 1943 Size: 298 7419 XmaI C'CCGG_G Cuts at: 1870 3532 3802 3835 5042 1870 Size: 1662 270 33 1207 4545 Fragments arranged by size: 4545 1662 1207 270 33 XmnI GAAnn'nnTTC Cuts at: 5846 5846 Size: 7717 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2299 4717 5525 276 Size: 53 83 186 936 765 2418 808 2468 Fragments arranged by size: 2468 2418 936 808 765 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PspOMI PsrI RsrII SacII SanDI SapI SfiI SwaI