Restriction map for
pFN21AE3342
(Circular) (Six-base) MAPSORT of: pFN21AE3342.seq Check: 4203 from: 1 to: 7666
pFN21AE3342 7666 bp
With 182 enzymes: SgfI *
March 28, 2012 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3863 3863
Size: 7666
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3467 5476 278
Size: 53 83 186 936 1931 2009 2468
Fragments arranged by size:
2468 2009 1931 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3771 5009 1937
Size: 1834 1238 4594
Fragments arranged by size:
4594 1834 1238
Acc65I G'GTAC_C
Cuts at: 3301 4191 4987 3301
Size: 890 796 5980
Fragments arranged by size:
5980 890 796
AclI AA'CG_TT
Cuts at: 1563 5793 6166 1563
Size: 4230 373 3063
Fragments arranged by size:
4230 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 3479 3515 3971 5729 7113 7381 1632
Size: 482 1365 36 456 1758 1384 268 1917
Fragments arranged by size:
1917 1758 1384 1365 482 456 268 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7469
AflIII A'CryG_T
Cuts at: 1203 2259 6565 7542 1203
Size: 1056 4306 977 1327
Fragments arranged by size:
4306 1327 1056 977
AgeI A'CCGG_T
Cuts at: 6497 6497
Size: 7666
AhdI GACnn_n'nnGTC
Cuts at: 2496 6395 2496
Size: 3899 3767
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7666
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2579 3703 1184
Size: 1395 1124 5147
Fragments arranged by size:
5147 1395 1124
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2999 3031 3224 3256 2999
Size: 32 193 32 7409
Fragments arranged by size:
7409 193 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4796 5409 6981 4796
Size: 613 1572 5481
Fragments arranged by size:
5481 1572 613
ApaI G_GGCC'C
Cuts at: 3261 3555 3261
Size: 294 7372
ApaLI G'TGCA_C
Cuts at: 2955 5723 6879 2955
Size: 2768 1156 3742
Fragments arranged by size:
3742 2768 1156
ApoI r'AATT_y
Cuts at: 1493 3818 4975 5201 5353 7462 1493
Size: 2325 1157 226 152 2109 1697
Fragments arranged by size:
2325 2109 1697 1157 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6220 160
Size: 872 5188 1606
Fragments arranged by size:
5188 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7666
AvaI C'yCGr_G
Cuts at: 1870 1943 2203 2288 2307 2640 2949 3153 3375
Size: 73 260 85 19 333 309 204 222
Cuts at: 3375 4180 4668 4933 4991 1870
Size: 805 488 265 58 4545
Fragments arranged by size:
4545 805 488 333 309 265 260 222
204 85 73 58 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3753 3786 3753
Size: 33 7633
BamHI G'GATC_C
Cuts at: 4996 5363 4996
Size: 367 7299
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2549 2825 3301 3379
Size: 325 217 627 462 300 276 476 78
Cuts at: 3379 3428 4100 4191 4987 6442 618
Size: 49 672 91 796 1455 1842
Fragments arranged by size:
1842 1455 796 672 627 476 462 325
300 276 217 91 78 49
BanII G_rGCy'C
Cuts at: 1391 2013 3171 3261 3555 4334 4985 1391
Size: 622 1158 90 294 779 651 4072
Fragments arranged by size:
4072 1158 779 651 622 294 90
BbeI G_GCGC'C
Cuts at: 2253 3432 4104 2253
Size: 1179 672 5815
Fragments arranged by size:
5815 1179 672
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2335 3467 3801 928
Size: 971 436 1132 334 4793
Fragments arranged by size:
4793 1132 971 436 334
BbvCI CC'TCA_GC
Cuts at: 2790 4836 2790
Size: 2046 5620
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3622 3656 3708 3742 5857 5891 1903
Size: 34 1685 34 52 34 2115 34 3678
Fragments arranged by size:
3678 2115 1685 52 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3250 5558 6768 3250
Size: 2308 1210 4148
Fragments arranged by size:
4148 2308 1210
BclI T'GATC_A
Cuts at: 1548 4491 1548
Size: 2943 4723
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2558 3009 4044
Size: 107 122 71 631 605 886 451 1035
Cuts at: 4044 4373 6277 136
Size: 329 1904 1525
Fragments arranged by size:
1904 1525 1035 886 631 605 451 329
122 107 71
BglII A'GATC_T
Cuts at: 4827 7661 4827
Size: 2834 4832
BlpI GC'TnA_GC
Cuts at: 2510 5083 2510
Size: 2573 5093
Bme1580I G_kGCm'C
Cuts at: 1790 2203 2658 2821 2828 2938 2959 3040 3261
Size: 413 455 163 7 110 21 81 221
Cuts at: 3261 3382 3555 3635 5727 6883 1790
Size: 121 173 80 2092 1156 2573
Fragments arranged by size:
2573 2092 1156 455 413 221 173 163
121 110 81 80 21 7
BmgBI CAC'GTC
Cuts at: 1318 2191 4489 1318
Size: 873 2298 4495
Fragments arranged by size:
4495 2298 873
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 4477 5397
Size: 416 53 83 399 24 1961 1099 920
Cuts at: 5397 6355 442
Size: 958 1753
Fragments arranged by size:
1961 1753 1099 958 920 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7666
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3083 4324 3083
Size: 1241 6425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3065 4061 4319 4435 6326 1239
Size: 1826 996 258 116 1891 2579
Fragments arranged by size:
2579 1891 1826 996 258 116
Bpu10I CC'TnA_GC
Cuts at: 2790 3247 4836 2790
Size: 457 1589 5620
Fragments arranged by size:
5620 1589 457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3373 3890 5791 6656 6954 3373
Size: 517 1901 865 298 4085
Fragments arranged by size:
4085 1901 865 517 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2051 2324 3123 4215 6329 882
Size: 617 552 273 799 1092 2114 2219
Fragments arranged by size:
2219 2114 1092 799 617 552 273
BsaAI yAC'GTr
Cuts at: 493 1204 2260 5457 493
Size: 711 1056 3197 2702
Fragments arranged by size:
3197 2702 1056 711
BsaBI GATnn'nnATC
Cuts at: 4352 7660 4352
Size: 3308 4358
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2250 3429 3464 4101
Size: 53 83 186 936 717 1179 35 637
Cuts at: 4101 5473 5855 6490 275
Size: 1372 382 635 1451
Fragments arranged by size:
1451 1372 1179 936 717 637 635 382
186 83 53 35
BsaWI w'CCGG_w
Cuts at: 4386 4584 4638 6099 6497 6771 6918 7329 4386
Size: 198 54 1461 398 274 147 411 4723
Fragments arranged by size:
4723 1461 411 398 274 198 147 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2956 2986 2999 3029 4316
Size: 30 165 30 1553 30 13 30 1287
Cuts at: 4316 4346 4430 4448 4460 4478 4680 4710 1178
Size: 30 84 18 12 18 202 30 4134
Fragments arranged by size:
4134 1553 1287 202 165 84 30 30
30 30 30 30 18 18 13 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3004 4316 1186
Size: 1818 1312 4536
Fragments arranged by size:
4536 1818 1312
BseYI C'CCAG_C
Cuts at: 2028 2312 2598 2702 2708 2869 3344 3440 6869
Size: 284 286 104 6 161 475 96 3429
Cuts at: 6869 7365 2028
Size: 496 2329
Fragments arranged by size:
3429 2329 496 475 286 284 161 104
96 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2213 2558 2947 3005 3539 3891 1163
Size: 573 477 345 389 58 534 352 4938
Fragments arranged by size:
4938 573 534 477 389 352 345 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5116 5877 6905 1540
Size: 275 186 3115 761 1028 2301
Fragments arranged by size:
3115 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2013 2773 2959 4023 4334 5727 5812 6883 2013
Size: 760 186 1064 311 1393 85 1071 2796
Fragments arranged by size:
2796 1393 1071 1064 760 311 186 85
BsmI GAATG_Cn'
Cuts at: 4594 5177 5270 4594
Size: 583 93 6990
Fragments arranged by size:
6990 583 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2203 2658 2773 2821 2828 2938
Size: 399 223 190 455 115 48 7 110
Cuts at: 2938 2959 3040 3171 3261 3382 3555 3635 4023
Size: 21 81 131 90 121 173 80 388
Cuts at: 4023 4334 4985 5727 5812 6883 1391
Size: 311 651 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 455 399 388 311
223 190 173 131 121 115 110 90
85 81 80 48 21 7
BspEI T'CCGG_A
Cuts at: 4386 4638 7329 4386
Size: 252 2691 4723
Fragments arranged by size:
4723 2691 252
BspHI T'CATG_A
Cuts at: 4071 5555 7285 4071
Size: 1484 1730 4452
Fragments arranged by size:
4452 1730 1484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3511 3863 4851 5021 844
Size: 2667 352 988 170 3489
Fragments arranged by size:
3489 2667 988 352 170
BsrBI CCG'CTC
Cuts at: 1394 1823 2142 2509 3970 5553 1394
Size: 429 319 367 1461 1583 3507
Fragments arranged by size:
3507 1583 1461 429 367 319
BsrDI GCAATG_nn'
Cuts at: 65 1248 3712 4377 6161 6335 65
Size: 1183 2464 665 1784 174 1396
Fragments arranged by size:
2464 1784 1396 1183 665 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2663 2749 3542 4515 4951 6310 6497
Size: 110 853 86 793 973 436 1359 187
Cuts at: 6497 1700
Size: 2869
Fragments arranged by size:
2869 1359 973 853 793 436 187 110
86
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7666
BssHII G'CGCG_C
Cuts at: 1925 3530 1925
Size: 1605 6061
BssSI C'ACGA_G
Cuts at: 4154 4327 5726 6738 4154
Size: 173 1399 1012 5082
Fragments arranged by size:
5082 1399 1012 173
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2167 794
Size: 1373 6293
BstBI TT'CG_AA
Cuts at: 6470 6470
Size: 7666
BstEII G'GTnAC_C
Cuts at: 1184 4051 1184
Size: 2867 4799
BstXI CCAn_nnnn'nTGG
Cuts at: 3514 4765 3514
Size: 1251 6415
BstYI r'GATC_y
Cuts at: 1972 4827 4996 5363 5748 5765 7217 7303 7315
Size: 2855 169 367 385 17 1452 86 12
Cuts at: 7315 7661 1972
Size: 346 1977
Fragments arranged by size:
2855 1977 1452 385 367 346 169 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2112 3692 2112
Size: 1580 6086
BtgI C'CryG_G
Cuts at: 513 1065 2004 2240 2373 2430 2658 2984 3252
Size: 552 939 236 133 57 228 326 268
Cuts at: 3252 513
Size: 4927
Fragments arranged by size:
4927 939 552 326 268 236 228 133
57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2373 4159 487
Size: 683 56 1147 1786 3994
Fragments arranged by size:
3994 1786 1147 683 56
BtsI GCAGTG_nn'
Cuts at: 2132 3222 3318 5182 5976 5996 2132
Size: 1090 96 1864 794 20 3802
Fragments arranged by size:
3802 1864 1090 794 96 20
ClaI AT'CG_AT
Cuts at: 1554 3834 1554
Size: 2280 5386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7631
DraI TTT'AAA
Cuts at: 4970 4970
Size: 7666
DraIII CAC_nnn'GTG
Cuts at: 2167 2805 3386 2167
Size: 638 581 6447
Fragments arranged by size:
6447 638 581
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3407 6673 809
Size: 507 2091 3266 1802
Fragments arranged by size:
3266 2091 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2370 2427
Size: 54 1425 50 275 18 210 330 57
Cuts at: 2427 2661 2731 3055 3559 4890 5113 6002 7631
Size: 234 70 324 504 1331 223 889 1629
Cuts at: 7631 8
Size: 43
Fragments arranged by size:
1629 1425 1331 889 504 330 324 275
234 223 210 70 57 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5113 1537
Size: 275 3301 4090
Fragments arranged by size:
4090 3301 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 2585 3852 4315 5596 1353
Size: 696 536 1267 463 1281 3423
Fragments arranged by size:
3423 1281 1267 696 536 463
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4025 4390 6253 6627 6773 387
Size: 1064 138 2436 365 1863 374 146 1280
Fragments arranged by size:
2436 1863 1280 1064 374 365 146 138
EcoICRI GAG'CTC
Cuts at: 2011 4332 2011
Size: 2321 5345
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 3065 3479 3515 3971 4061 4319
Size: 393 482 951 414 36 456 90 258
Cuts at: 4319 4435 5729 6326 7113 7381 1239
Size: 116 1294 597 787 268 1524
Fragments arranged by size:
1524 1294 951 787 597 482 456 414
393 268 258 116 90 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 3518 2150
Size: 1368 6298
EcoO109I rG'GnC_Cy
Cuts at: 2236 2853 3064 3157 3257 3437 3552 3910 4683
Size: 617 211 93 100 180 115 358 773
Cuts at: 4683 4840 2236
Size: 157 5062
Fragments arranged by size:
5062 773 617 358 211 180 157 115
100 93
EcoRI G'AATT_C
Cuts at: 4975 5353 4975
Size: 378 7288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2539 4903 2350
Size: 189 2364 5113
Fragments arranged by size:
5113 2364 189
FspI TGC'GCA
Cuts at: 2171 6172 2171
Size: 4001 3665
FspAI rTGC'GCAy
Cuts at: 2171 2171
Size: 7666
HaeII r_GCGC'y
Cuts at: 1678 2253 2320 2455 3432 4104 4623 6813 1678
Size: 575 67 135 977 672 519 2190 2531
Fragments arranged by size:
2531 2190 977 672 575 519 135 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2095 2127 2282 2314 2482
Size: 32 135 32 523 32 155 32 168
Cuts at: 2482 2514 2623 2655 2889 2921 3732 3764 4430
Size: 32 109 32 234 32 811 32 666
Cuts at: 4430 4462 6307 6339 6381 6413 1373
Size: 32 1845 32 42 32 2626
Fragments arranged by size:
2626 1845 811 666 523 234 168 155
135 109 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4249 5010 5256 669
Size: 988 281 2311 761 246 3079
Fragments arranged by size:
3079 2311 988 761 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7666
HpaI GTT'AAC
Cuts at: 5256 5256
Size: 7666
KasI G'GCGC_C
Cuts at: 2249 3428 4100 2249
Size: 1179 672 5815
Fragments arranged by size:
5815 1179 672
KpnI G_GTAC'C
Cuts at: 3305 4195 4991 3305
Size: 890 796 5980
Fragments arranged by size:
5980 890 796
MfeI C'AATT_G
Cuts at: 5265 5265
Size: 7666
MluI A'CGCG_T
Cuts at: 7542 7542
Size: 7666
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3342 6780 6964 1305
Size: 2037 3438 184 2007
Fragments arranged by size:
3438 2037 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2733 4892 10
Size: 54 1425 343 901 2159 2784
Fragments arranged by size:
2784 2159 1425 901 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 3326 4763 5624 5983 6142 518
Size: 648 1248 912 1437 861 359 159 2042
Fragments arranged by size:
2042 1437 1248 912 861 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2432 2602 2766 2789 2986 3084 3616 3976
Size: 826 170 164 23 197 98 532 360
Cuts at: 3976 4708 4796 5082 5759 6907 7152 1606
Size: 732 88 286 677 1148 245 2120
Fragments arranged by size:
2120 1148 826 732 677 532 360 286
245 197 170 164 98 88 23
NaeI GCC'GGC
Cuts at: 3544 3544
Size: 7666
NarI GG'CG_CC
Cuts at: 2250 3429 4101 2250
Size: 1179 672 5815
Fragments arranged by size:
5815 1179 672
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6175
Fragments arranged by size:
6175 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7666
NgoMIV G'CCGG_C
Cuts at: 3542 3542
Size: 7666
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7666
NotI GC'GGCC_GC
Cuts at: 5113 5113
Size: 7666
NspI r_CATG'y
Cuts at: 1132 1229 4066 4165 4189 5024 6569 1132
Size: 97 2837 99 24 835 1545 2229
Fragments arranged by size:
2837 2229 1545 835 99 97 24
PasI CC'CwG_GG
Cuts at: 1348 2456 2821 3171 3914 4336 1348
Size: 1108 365 350 743 422 4678
Fragments arranged by size:
4678 1108 743 422 365 350
PciI A'CATG_T
Cuts at: 6565 6565
Size: 7666
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4156 4359 1108
Size: 3048 203 4415
Fragments arranged by size:
4415 3048 203
PfoI T'CCnGG_A
Cuts at: 2538 2625 4296 4698 2538
Size: 87 1671 402 5506
Fragments arranged by size:
5506 1671 402 87
PmeI GTTT'AAAC
Cuts at: 4970 4970
Size: 7666
PmlI CAC'GTG
Cuts at: 2260 2260
Size: 7666
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2999 3031 5735 5767 7248 7280 2999
Size: 32 2704 32 1481 32 3385
Fragments arranged by size:
3385 2704 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2236 2853 3064 3157 3437 4840 2236
Size: 617 211 93 280 1403 5062
Fragments arranged by size:
5062 1403 617 280 211 93
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7666
PsiI TTA'TAA
Cuts at: 5236 5236
Size: 7666
PspOMI G'GGCC_C
Cuts at: 3257 3551 3257
Size: 294 7372
PspXI vC'TCGA_Gb
Cuts at: 2288 2288
Size: 7666
PstI C_TGCA'G
Cuts at: 830 2765 3313 5018 830
Size: 1935 548 1705 3478
Fragments arranged by size:
3478 1935 1705 548
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7666
PvuII CAG'CTG
Cuts at: 2602 2766 2789 3616 2602
Size: 164 23 827 6652
Fragments arranged by size:
6652 827 164 23
SacI G_AGCT'C
Cuts at: 2013 4334 2013
Size: 2321 5345
SacII CC_GC'GG
Cuts at: 2433 2987 2433
Size: 554 7112
SalI G'TCGA_C
Cuts at: 1936 5008 1936
Size: 3072 4594
SanDI GG'GwC_CC
Cuts at: 2236 3437 2236
Size: 1201 6465
SbfI CC_TGCA'GG
Cuts at: 5018 5018
Size: 7666
ScaI AGT'ACT
Cuts at: 5914 7626 5914
Size: 1712 5954
SexAI A'CCwGG_T
Cuts at: 3159 3513 3159
Size: 354 7312
SfcI C'TryA_G
Cuts at: 826 1045 2761 3309 5014 6149 6830 7021 826
Size: 219 1716 548 1705 1135 681 191 1471
Fragments arranged by size:
1716 1705 1471 1135 681 548 219 191
SfoI GGC'GCC
Cuts at: 2251 3430 4102 2251
Size: 1179 672 5815
Fragments arranged by size:
5815 1179 672
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7666
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7666
SmaI CCC'GGG
Cuts at: 1872 2205 2951 3155 3377 4670 4935 4993 1872
Size: 333 746 204 222 1293 265 58 4545
Fragments arranged by size:
4545 1293 746 333 265 222 204 58
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2288 3352 3905 5770 6671 6933
Size: 197 926 345 1064 553 1865 901 262
Cuts at: 6933 820
Size: 1553
Fragments arranged by size:
1865 1553 1064 926 901 553 345 262
197
SnaBI TAC'GTA
Cuts at: 493 5457 493
Size: 4964 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7666
SphI G_CATG'C
Cuts at: 1132 4066 4165 4189 5024 1132
Size: 2934 99 24 835 3774
Fragments arranged by size:
3774 2934 835 99 24
SspI AAT'ATT
Cuts at: 5 52 3725 5590 5
Size: 47 3673 1865 2081
Fragments arranged by size:
3673 2081 1865 47
StuI AGG'CCT
Cuts at: 1517 3998 1517
Size: 2481 5185
StyI C'CwwG_G
Cuts at: 513 1065 2004 5105 513
Size: 552 939 3101 3074
Fragments arranged by size:
3101 3074 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2089 3163 3309 3358 3397 3635 5693
Size: 1255 509 1074 146 49 39 238 2058
Cuts at: 5693 5710 5863 6048 6493 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2058 1498 1255 1074 509 445 238 185
153 146 49 39 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5912 7624 96
Size: 274 80 33 51 1443 3935 1712 138
Fragments arranged by size:
3935 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2111 2143 2720 2752 2956 2988 4680 4712 4895
Size: 32 577 32 204 32 1692 32 183
Cuts at: 4895 4927 7626 7658 2111
Size: 32 2699 32 2119
Fragments arranged by size:
2699 2119 1692 577 204 183 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3334 1316
Size: 2018 5648
XbaI T'CTAG_A
Cuts at: 5002 5002
Size: 7666
XhoI C'TCGA_G
Cuts at: 1943 2288 1943
Size: 345 7321
XmaI C'CCGG_G
Cuts at: 1870 2203 2949 3153 3375 4668 4933 4991 1870
Size: 333 746 204 222 1293 265 58 4545
Fragments arranged by size:
4545 1293 746 333 265 222 204 58
XmnI GAAnn'nnTTC
Cuts at: 5795 5795
Size: 7666
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3465 5474 276
Size: 53 83 186 936 1931 2009 2468
Fragments arranged by size:
2468 2009 1931 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BsiWI BsmBI BstZ17I EcoRV
FseI NruI NsiI PacI PsrI RsrII SapI SfiI
SrfI SwaI XcmI