Restriction map for
pFN21AE3340
(Circular) (Six-base) MAPSORT of: pFN21AE3340.seq Check: 7174 from: 1 to: 7657
pFN21AE3340 7657 bp
With 182 enzymes: SgfI *
March 28, 2012 12:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2774 2774
Size: 7657
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3014 5467 278
Size: 53 83 186 936 1478 2453 2468
Fragments arranged by size:
2468 2453 1478 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2415 5000 1937
Size: 478 2585 4594
Fragments arranged by size:
4594 2585 478
Acc65I G'GTAC_C
Cuts at: 4978 4978
Size: 7657
AclI AA'CG_TT
Cuts at: 1563 2066 5784 6157 1563
Size: 503 3718 373 3063
Fragments arranged by size:
3718 3063 503 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2868 3983 4395 5720 7104 7372 1632
Size: 1236 1115 412 1325 1384 268 1917
Fragments arranged by size:
1917 1384 1325 1236 1115 412 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7460
AflIII A'CryG_T
Cuts at: 1203 6556 7533 1203
Size: 5353 977 1327
Fragments arranged by size:
5353 1327 977
AgeI A'CCGG_T
Cuts at: 2918 6488 2918
Size: 3570 4087
AhdI GACnn_n'nnGTC
Cuts at: 6386 6386
Size: 7657
AleI CACnn'nnGTG
Cuts at: 1166 4691 1166
Size: 3525 4132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2628 1184
Size: 1444 6213
AlwNI CAG_nnn'CTG
Cuts at: 2051 3580 4708 5400 6972 2051
Size: 1529 1128 692 1572 2736
Fragments arranged by size:
2736 1572 1529 1128 692
ApaI G_GGCC'C
Cuts at: 2251 3199 2251
Size: 948 6709
ApaLI G'TGCA_C
Cuts at: 5714 6870 5714
Size: 1156 6501
ApoI r'AATT_y
Cuts at: 1493 2608 4966 5192 5344 7453 1493
Size: 1115 2358 226 152 2109 1697
Fragments arranged by size:
2358 2109 1697 1115 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6211 160
Size: 872 5179 1606
Fragments arranged by size:
5179 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7657
AvaI C'yCGr_G
Cuts at: 1870 1943 2667 4982 1870
Size: 73 724 2315 4545
Fragments arranged by size:
4545 2315 724 73
BamHI G'GATC_C
Cuts at: 2315 3808 4987 5354 2315
Size: 1493 1179 367 4618
Fragments arranged by size:
4618 1493 1179 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2746 3030 4978 6433 618
Size: 325 217 627 959 284 1948 1455 1842
Fragments arranged by size:
1948 1842 1455 959 627 325 284 217
BanII G_rGCy'C
Cuts at: 1391 2202 2251 3199 4976 1391
Size: 811 49 948 1777 4072
Fragments arranged by size:
4072 1777 948 811 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2185 2569 4857 928
Size: 971 286 384 2288 3728
Fragments arranged by size:
3728 2288 971 384 286
BbvCI CC'TCA_GC
Cuts at: 3608 3608
Size: 7657
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2176 2210 2748 2782 3957 3991 5848
Size: 34 239 34 538 34 1175 34 1857
Cuts at: 5848 5882 1903
Size: 34 3678
Fragments arranged by size:
3678 1857 1175 538 239 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5549 6759 5549
Size: 1210 6447
BclI T'GATC_A
Cuts at: 1548 4086 1548
Size: 2538 5119
BfrBI ATG'CAT
Cuts at: 2679 2679
Size: 7657
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2755 6268 136
Size: 107 122 71 631 605 1083 3513 1525
Fragments arranged by size:
3513 1525 1083 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3780 7652 3780
Size: 3872 3785
BlpI GC'TnA_GC
Cuts at: 4763 5074 4763
Size: 311 7346
Bme1580I G_kGCm'C
Cuts at: 1790 2251 2675 2709 3147 3199 3529 5718 6874
Size: 461 424 34 438 52 330 2189 1156
Cuts at: 6874 1790
Size: 2573
Fragments arranged by size:
2573 2189 1156 461 438 424 330 52
34
BmgBI CAC'GTC
Cuts at: 1318 2704 3271 1318
Size: 1386 567 5704
Fragments arranged by size:
5704 1386 567
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5388 6346 442
Size: 416 53 83 399 24 3971 958 1753
Fragments arranged by size:
3971 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7657
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2458 2670 3460 2374
Size: 84 212 790 6571
Fragments arranged by size:
6571 790 212 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2931 3044 3226 3725 4073 4295 4365 4808
Size: 1692 113 182 499 348 222 70 443
Cuts at: 4808 6317 1239
Size: 1509 2579
Fragments arranged by size:
2579 1692 1509 499 443 348 222 182
113 70
Bpu10I CC'TnA_GC
Cuts at: 3608 4178 3608
Size: 570 7087
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4652 5782 6647 6945 4652
Size: 1130 865 298 5364
Fragments arranged by size:
5364 1130 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2321 4465 6320 882
Size: 617 822 2144 1855 2219
Fragments arranged by size:
2219 2144 1855 822 617
BsaAI yAC'GTr
Cuts at: 493 1204 3946 5448 493
Size: 711 2742 1502 2702
Fragments arranged by size:
2742 2702 1502 711
BsaBI GATnn'nnATC
Cuts at: 3779 7651 3779
Size: 3872 3785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2894 3011 5464 5846
Size: 53 83 186 936 1361 117 2453 382
Cuts at: 5846 6481 275
Size: 635 1451
Fragments arranged by size:
2453 1451 1361 936 635 382 186 117
83 53
BsaWI w'CCGG_w
Cuts at: 2918 3411 4596 4650 6090 6488 6762 6909 7320
Size: 493 1185 54 1440 398 274 147 411
Cuts at: 7320 2918
Size: 3255
Fragments arranged by size:
3255 1440 1185 493 411 398 274 147
54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2013 2016 2043 2046 2852
Size: 30 165 30 610 3 27 3 806
Cuts at: 2852 2864 2882 2894 4328 4358 1178
Size: 12 18 12 1434 30 4477
Fragments arranged by size:
4477 1434 806 610 165 30 30 30
27 18 12 12 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2021 3361 3436 1186
Size: 835 1340 75 5407
Fragments arranged by size:
5407 1340 835 75
BseYI C'CCAG_C
Cuts at: 2098 2594 3154 4701 6860 7356 2098
Size: 496 560 1547 2159 496 2399
Fragments arranged by size:
2399 2159 1547 560 496 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2890 1163
Size: 573 1154 5930
Fragments arranged by size:
5930 1154 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3648 4717 5107 5868 6896 1540
Size: 275 186 1647 1069 390 761 1028 2301
Fragments arranged by size:
2301 1647 1069 1028 761 390 275 186
BsiHKAI G_wGCw'C
Cuts at: 2651 5718 5803 6874 2651
Size: 3067 85 1071 3434
Fragments arranged by size:
3434 3067 1071 85
BsmI GAATG_Cn'
Cuts at: 4908 5168 5261 4908
Size: 260 93 7304
Fragments arranged by size:
7304 260 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2901 3002 3181 3277 2901
Size: 101 179 96 7281
Fragments arranged by size:
7281 179 101 96
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2251 2651 2675 2709 3147 3199
Size: 399 412 49 400 24 34 438 52
Cuts at: 3199 3529 4976 5718 5803 6874 1391
Size: 330 1447 742 85 1071 2174
Fragments arranged by size:
2174 1447 1071 742 438 412 400 399
330 85 52 49 34 24
BspEI T'CCGG_A
Cuts at: 4650 7320 4650
Size: 2670 4987
BspHI T'CATG_A
Cuts at: 4083 5546 7276 4083
Size: 1463 1730 4464
Fragments arranged by size:
4464 1730 1463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2774 5012 844
Size: 1930 2238 3489
Fragments arranged by size:
3489 2238 1930
BsrBI CCG'CTC
Cuts at: 1394 1823 2880 2933 4719 5544 1394
Size: 429 1057 53 1786 825 3507
Fragments arranged by size:
3507 1786 1057 825 429 53
BsrDI GCAATG_nn'
Cuts at: 65 1248 2194 2364 2421 2992 4956 6152 6326
Size: 1183 946 170 57 571 1964 1196 174
Cuts at: 6326 65
Size: 1396
Fragments arranged by size:
1964 1396 1196 1183 946 571 174 170
57
BsrFI r'CCGG_y
Cuts at: 1700 1810 2238 2918 4527 4605 6301 6488 1700
Size: 110 428 680 1609 78 1696 187 2869
Fragments arranged by size:
2869 1696 1609 680 428 187 110 78
BsrGI T'GTAC_A
Cuts at: 96 3726 3903 96
Size: 3630 177 3850
Fragments arranged by size:
3850 3630 177
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7657
BssSI C'ACGA_G
Cuts at: 5717 6729 5717
Size: 1012 6645
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2051 2811 3090 3567 794
Size: 1257 760 279 477 4884
Fragments arranged by size:
4884 1257 760 477 279
BstBI TT'CG_AA
Cuts at: 6461 6461
Size: 7657
BstEII G'GTnAC_C
Cuts at: 1184 4063 4831 1184
Size: 2879 768 4010
Fragments arranged by size:
4010 2879 768
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3022 3154 4348 4777 2011
Size: 1011 132 1194 429 4891
Fragments arranged by size:
4891 1194 1011 429 132
BstYI r'GATC_y
Cuts at: 1972 2315 3408 3780 3808 4327 4359 4896 4987
Size: 343 1093 372 28 519 32 537 91
Cuts at: 4987 5354 5739 5756 7208 7294 7306 7652 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1093 537 519 385 372 367
346 343 91 86 32 28 17 12
Bsu36I CC'TnA_GG
Cuts at: 4130 4130
Size: 7657
BtgI C'CryG_G
Cuts at: 513 1065 2004 3450 4542 4749 513
Size: 552 939 1446 1092 207 3421
Fragments arranged by size:
3421 1446 1092 939 552 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2069 2237 2773 4171 4375 487
Size: 683 56 843 168 536 1398 204 3769
Fragments arranged by size:
3769 1398 843 683 536 204 168 56
BtsI GCAGTG_nn'
Cuts at: 2052 2622 2860 4803 5173 5967 5987 2052
Size: 570 238 1943 370 794 20 3722
Fragments arranged by size:
3722 1943 794 570 370 238 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7657
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3538 3573 529
Size: 35 2974 35 4613
Fragments arranged by size:
4613 2974 35 35
DraI TTT'AAA
Cuts at: 4961 4961
Size: 7657
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6664 809
Size: 507 5348 1802
Fragments arranged by size:
5348 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 3365 3453
Size: 54 1425 50 275 18 279 1256 88
Cuts at: 3453 3645 3675 3948 5104 5993 7622 8
Size: 192 30 273 1156 889 1629 43
Fragments arranged by size:
1629 1425 1256 1156 889 279 275 273
192 88 54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3645 5104 1537
Size: 275 1833 1459 4090
Fragments arranged by size:
4090 1833 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2565 3864 5587 1353
Size: 1212 1299 1723 3423
Fragments arranged by size:
3423 1723 1299 1212
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6244 6618 6764 387
Size: 1064 138 4655 374 146 1280
Fragments arranged by size:
4655 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2868 2931 3044 3226 3725 3983 4073
Size: 393 1236 63 113 182 499 258 90
Cuts at: 4073 4295 4365 4395 4808 5720 6317 7104 7372
Size: 222 70 30 413 912 597 787 268
Cuts at: 7372 1239
Size: 1524
Fragments arranged by size:
1524 1236 912 787 597 499 413 393
268 258 222 182 113 90 70 63
30
EcoO109I rG'GnC_Cy
Cuts at: 2247 2398 2978 4234 2247
Size: 151 580 1256 5670
Fragments arranged by size:
5670 1256 580 151
EcoRI G'AATT_C
Cuts at: 4966 5344 4966
Size: 378 7279
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2347 2401 3271 3586 2347
Size: 54 870 315 6418
Fragments arranged by size:
6418 870 315 54
FspI TGC'GCA
Cuts at: 6163 6163
Size: 7657
HaeII r_GCGC'y
Cuts at: 1678 4770 6804 1678
Size: 3092 2034 2531
Fragments arranged by size:
3092 2531 2034
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2098 2130 2636 2668 3584
Size: 32 135 32 526 32 506 32 916
Cuts at: 3584 3616 4307 4339 4344 4376 6298 6330 6372
Size: 32 691 32 5 32 1922 32 42
Cuts at: 6372 6404 1373
Size: 32 2626
Fragments arranged by size:
2626 1922 916 691 526 506 135 42
32 32 32 32 32 32 32 32
32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3280 4261 4630 5001 5247 669
Size: 988 281 1342 981 369 371 246 3079
Fragments arranged by size:
3079 1342 988 981 371 369 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7657
HpaI GTT'AAC
Cuts at: 5247 5247
Size: 7657
KpnI G_GTAC'C
Cuts at: 4982 4982
Size: 7657
MfeI C'AATT_G
Cuts at: 2212 5256 2212
Size: 3044 4613
MluI A'CGCG_T
Cuts at: 7533 7533
Size: 7657
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2894 2895 4311 4515 6771 6955 1305
Size: 1589 1 1416 204 2256 184 2007
Fragments arranged by size:
2256 2007 1589 1416 204 184 1
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3367 3455 3677 3950 10
Size: 54 1425 343 1535 88 222 273 3717
Fragments arranged by size:
3717 1535 1425 343 273 222 88 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 4691 4775 4838 5615 5974 6133
Size: 648 1101 2424 84 63 777 359 159
Cuts at: 6133 518
Size: 2042
Fragments arranged by size:
2424 2042 1101 777 648 359 159 84
63
MspA1I CmG'CkG
Cuts at: 1606 3120 3154 3619 3644 4556 4828 5073 5750
Size: 1514 34 465 25 912 272 245 677
Cuts at: 5750 6898 7143 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1514 1148 912 677 465 272 245
245 34 25
NaeI GCC'GGC
Cuts at: 4607 4607
Size: 7657
NcoI C'CATG_G
Cuts at: 513 1065 2004 3450 4542 513
Size: 552 939 1446 1092 3628
Fragments arranged by size:
3628 1446 1092 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7657
NgoMIV G'CCGG_C
Cuts at: 4605 4605
Size: 7657
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7657
NotI GC'GGCC_GC
Cuts at: 5104 5104
Size: 7657
NsiI A_TGCA'T
Cuts at: 2681 2681
Size: 7657
NspI r_CATG'y
Cuts at: 1132 1229 2679 2805 2961 5015 6560 1132
Size: 97 1450 126 156 2054 1545 2229
Fragments arranged by size:
2229 2054 1545 1450 156 126 97
PasI CC'CwG_GG
Cuts at: 1348 3770 1348
Size: 2422 5235
PciI A'CATG_T
Cuts at: 6556 6556
Size: 7657
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4168 1108
Size: 3060 4597
PfoI T'CCnGG_A
Cuts at: 3396 3825 4311 3396
Size: 429 486 6742
Fragments arranged by size:
6742 486 429
PmeI GTTT'AAAC
Cuts at: 4961 4961
Size: 7657
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5726 5758 7239 7271 5726
Size: 32 1481 32 6112
Fragments arranged by size:
6112 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2978 4234 2978
Size: 1256 6401
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7657
PsiI TTA'TAA
Cuts at: 5227 5227
Size: 7657
PspOMI G'GGCC_C
Cuts at: 2247 3195 2247
Size: 948 6709
PstI C_TGCA'G
Cuts at: 830 2095 2294 2431 2821 2890 5009 830
Size: 1265 199 137 390 69 2119 3478
Fragments arranged by size:
3478 2119 1265 390 199 137 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7657
PvuII CAG'CTG
Cuts at: 3154 3619 4556 4828 3154
Size: 465 937 272 5983
Fragments arranged by size:
5983 937 465 272
RsrII CG'GwC_CG
Cuts at: 2916 2916
Size: 7657
SalI G'TCGA_C
Cuts at: 1936 4999 1936
Size: 3063 4594
SbfI CC_TGCA'GG
Cuts at: 2821 5009 2821
Size: 2188 5469
ScaI AGT'ACT
Cuts at: 5905 7617 5905
Size: 1712 5945
SexAI A'CCwGG_T
Cuts at: 2178 4269 2178
Size: 2091 5566
SfcI C'TryA_G
Cuts at: 826 1045 2091 2290 2427 2817 2886 3691 5005
Size: 219 1046 199 137 390 69 805 1314
Cuts at: 5005 6140 6821 7012 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1314 1135 1046 805 681 390 219
199 191 137 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7657
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7657
SmaI CCC'GGG
Cuts at: 1872 4984 1872
Size: 3112 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4667 5761 6662 6924 820
Size: 197 926 2724 1094 901 262 1553
Fragments arranged by size:
2724 1553 1094 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5448 493
Size: 4955 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7657
SphI G_CATG'C
Cuts at: 1132 2805 2961 5015 1132
Size: 1673 156 2054 3774
Fragments arranged by size:
3774 2054 1673 156
SspI AAT'ATT
Cuts at: 5 52 5581 5
Size: 47 5529 2081
Fragments arranged by size:
5529 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7657
StyI C'CwwG_G
Cuts at: 513 1065 2004 3038 3450 3669 3837 4347 4542
Size: 552 939 1034 412 219 168 510 195
Cuts at: 4542 4875 5096 513
Size: 333 221 3074
Fragments arranged by size:
3074 1034 939 552 510 412 333 221
219 195 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4555 4850 5684 5701 5854 6039 6484
Size: 1255 2975 295 834 17 153 185 445
Cuts at: 6484 325
Size: 1498
Fragments arranged by size:
2975 1498 1255 834 445 295 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3726 3903 4785
Size: 274 80 33 51 1443 1749 177 882
Cuts at: 4785 5903 7615 96
Size: 1118 1712 138
Fragments arranged by size:
1749 1712 1443 1118 882 274 177 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2852 2884 4312 4344 7617 7649 2852
Size: 32 1428 32 3273 32 2860
Fragments arranged by size:
3273 2860 1428 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2360 1316
Size: 1044 6613
XbaI T'CTAG_A
Cuts at: 4993 4993
Size: 7657
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2453 3230 2453
Size: 777 6880
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7657
XmaI C'CCGG_G
Cuts at: 1870 4982 1870
Size: 3112 4545
XmnI GAAnn'nnTTC
Cuts at: 5786 5786
Size: 7657
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3012 5465 276
Size: 53 83 186 936 1478 2453 2468
Fragments arranged by size:
2468 2453 1478 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI
NruI PacI PmlI PspXI PsrI SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI