Restriction map for
pFN21AE3338
(Circular) (Six-base) MAPSORT of: pFN21AE3338.seq Check: 2862 from: 1 to: 7630
pFN21AE3338 7630 bp
With 182 enzymes: SgfI *
March 28, 2012 12:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5440 278
Size: 53 83 186 936 3904 2468
Fragments arranged by size:
3904 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4973 1937
Size: 3036 4594
Acc65I G'GTAC_C
Cuts at: 4951 4951
Size: 7630
AclI AA'CG_TT
Cuts at: 1563 2332 5757 6130 1563
Size: 769 3425 373 3063
Fragments arranged by size:
3425 3063 769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2705 3113 3333 4397 5693 7077 7345 1632
Size: 1073 408 220 1064 1296 1384 268 1917
Fragments arranged by size:
1917 1384 1296 1073 1064 408 268 220
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7433
AflIII A'CryG_T
Cuts at: 1203 2533 4629 6529 7506 1203
Size: 1330 2096 1900 977 1327
Fragments arranged by size:
2096 1900 1330 1327 977
AgeI A'CCGG_T
Cuts at: 6461 6461
Size: 7630
AhdI GACnn_n'nnGTC
Cuts at: 6359 6359
Size: 7630
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7630
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2069 3408 1184
Size: 885 1339 5406
Fragments arranged by size:
5406 1339 885
AlwNI CAG_nnn'CTG
Cuts at: 2411 2665 3623 5373 6945 2411
Size: 254 958 1750 1572 3096
Fragments arranged by size:
3096 1750 1572 958 254
ApaLI G'TGCA_C
Cuts at: 5687 6843 5687
Size: 1156 6474
ApoI r'AATT_y
Cuts at: 1493 2844 2902 3377 4282 4939 5165 5317 7426
Size: 1351 58 475 905 657 226 152 2109
Cuts at: 7426 1493
Size: 1697
Fragments arranged by size:
2109 1697 1351 905 657 475 226 152
58
AseI AT'TA_AT
Cuts at: 160 1032 6184 160
Size: 872 5152 1606
Fragments arranged by size:
5152 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7630
AvaI C'yCGr_G
Cuts at: 1870 1943 4955 1870
Size: 73 3012 4545
Fragments arranged by size:
4545 3012 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2281 2302 2314 2335 2281
Size: 21 12 21 7576
Fragments arranged by size:
7576 21 21 12
BamHI G'GATC_C
Cuts at: 4960 5327 4960
Size: 367 7263
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 3236 4898 4951 6406
Size: 325 217 627 258 1191 1662 53 1455
Cuts at: 6406 618
Size: 1842
Fragments arranged by size:
1842 1662 1455 1191 627 325 258 217
53
BanII G_rGCy'C
Cuts at: 1391 2607 4651 4949 1391
Size: 1216 2044 298 4072
Fragments arranged by size:
4072 2044 1216 298
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2535 3284 4230 4385 4816 4850 928
Size: 971 636 749 946 155 431 34 3708
Fragments arranged by size:
3708 971 946 749 636 431 155 34
BbvCI CC'TCA_GC
Cuts at: 3305 3368 3646 3305
Size: 63 278 7289
Fragments arranged by size:
7289 278 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5821 5855 1903
Size: 34 3884 34 3678
Fragments arranged by size:
3884 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5522 6732 5522
Size: 1210 6420
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7630
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6241 136
Size: 107 122 71 631 605 4569 1525
Fragments arranged by size:
4569 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2713 4030 7625 2713
Size: 1317 3595 2718
Fragments arranged by size:
3595 2718 1317
BlpI GC'TnA_GC
Cuts at: 5047 5047
Size: 7630
Bme1580I G_kGCm'C
Cuts at: 1790 3559 5691 6847 1790
Size: 1769 2132 1156 2573
Fragments arranged by size:
2573 2132 1769 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7630
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3511 3565 3634
Size: 416 53 83 399 24 2094 54 69
Cuts at: 3634 5361 6319 442
Size: 1727 958 1753
Fragments arranged by size:
2094 1753 1727 958 416 399 83 69
54 53 24
BmtI G_CTAG'C
Cuts at: 1056 4160 1056
Size: 3104 4526
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2381 2686 4678 2381
Size: 305 1992 5333
Fragments arranged by size:
5333 1992 305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 2314 2363 2825 3062 3123 3437 4577
Size: 816 259 49 462 237 61 314 1140
Cuts at: 4577 4703 6290 1239
Size: 126 1587 2579
Fragments arranged by size:
2579 1587 1140 816 462 314 259 237
126 61 49
Bpu10I CC'TnA_GC
Cuts at: 3305 3368 3646 3825 3305
Size: 63 278 179 7110
Fragments arranged by size:
7110 278 179 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3020 3887 4031 4126 5755 6620 6918 3020
Size: 867 144 95 1629 865 298 3732
Fragments arranged by size:
3732 1629 867 865 298 144 95
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2352 4679 6293 882
Size: 617 853 2327 1614 2219
Fragments arranged by size:
2327 2219 1614 853 617
BsaAI yAC'GTr
Cuts at: 493 1204 5421 493
Size: 711 4217 2702
Fragments arranged by size:
4217 2702 711
BsaBI GATnn'nnATC
Cuts at: 2418 4911 7624 2418
Size: 2493 2713 2424
Fragments arranged by size:
2713 2493 2424
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5437 5819 6454 275
Size: 53 83 186 936 3904 382 635 1451
Fragments arranged by size:
3904 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6063 6461 6735 6882 7293 6063
Size: 398 274 147 411 6400
Fragments arranged by size:
6400 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3620 3650 4421 4451 1178
Size: 30 165 30 2217 30 771 30 4357
Fragments arranged by size:
4357 2217 771 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3017 3080 4142 4178 4364 1186
Size: 1831 63 1062 36 186 4452
Fragments arranged by size:
4452 1831 1062 186 63 36
BseYI C'CCAG_C
Cuts at: 4395 4471 4527 6833 7329 4395
Size: 76 56 2306 496 4696
Fragments arranged by size:
4696 2306 496 76 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2678 3132 1163
Size: 573 942 454 5661
Fragments arranged by size:
5661 942 573 454
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5080 5841 6869 1540
Size: 275 186 3079 761 1028 2301
Fragments arranged by size:
3079 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2229 2607 5691 5776 6847 2229
Size: 378 3084 85 1071 3012
Fragments arranged by size:
3084 3012 1071 378 85
BsmI GAATG_Cn'
Cuts at: 2995 5141 5234 2995
Size: 2146 93 5391
Fragments arranged by size:
5391 2146 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3035 3035
Size: 7630
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2607 3559 4651 4949 5691 5776
Size: 399 439 378 952 1092 298 742 85
Cuts at: 5776 6847 1391
Size: 1071 2174
Fragments arranged by size:
2174 1092 1071 952 742 439 399 378
298 85
BspEI T'CCGG_A
Cuts at: 7293 7293
Size: 7630
BspHI T'CATG_A
Cuts at: 5519 7249 5519
Size: 1730 5900
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2832 4985 844
Size: 1988 2153 3489
Fragments arranged by size:
3489 2153 1988
BsrBI CCG'CTC
Cuts at: 1394 1823 2930 5517 1394
Size: 429 1107 2587 3507
Fragments arranged by size:
3507 2587 1107 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4521 6125 6299 65
Size: 1183 3273 1604 174 1396
Fragments arranged by size:
3273 1604 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6274 6461 1700
Size: 110 4464 187 2869
Fragments arranged by size:
4464 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7630
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7630
BssSI C'ACGA_G
Cuts at: 2790 5690 6702 2790
Size: 2900 1012 3718
Fragments arranged by size:
3718 2900 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4261 794
Size: 3467 4163
BstBI TT'CG_AA
Cuts at: 2848 6434 2848
Size: 3586 4044
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7630
BstYI r'GATC_y
Cuts at: 1972 2713 4030 4051 4113 4320 4960 5327 5712
Size: 741 1317 21 62 207 640 367 385
Cuts at: 5712 5729 7181 7267 7279 7625 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1317 741 640 385 367 346
207 86 62 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 4161 513
Size: 552 3096 3982
Fragments arranged by size:
3982 3096 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2717 4898 487
Size: 683 56 1491 2181 3219
Fragments arranged by size:
3219 2181 1491 683 56
BtsI GCAGTG_nn'
Cuts at: 2153 2661 2834 3120 4128 5146 5940 5960 2153
Size: 508 173 286 1008 1018 794 20 3823
Fragments arranged by size:
3823 1018 1008 794 508 286 173 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7630
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7595
DraI TTT'AAA
Cuts at: 4934 4934
Size: 7630
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6637 809
Size: 507 5321 1802
Fragments arranged by size:
5321 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2490 4164 5077
Size: 54 1425 50 275 18 660 1674 913
Cuts at: 5077 5966 7595 8
Size: 889 1629 43
Fragments arranged by size:
1674 1629 1425 913 889 660 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5077 1537
Size: 275 3265 4090
Fragments arranged by size:
4090 3265 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2131 2502 4335 4416 4731 5560 1353
Size: 778 371 1833 81 315 829 3423
Fragments arranged by size:
3423 1833 829 778 371 315 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6217 6591 6737 387
Size: 1064 138 4628 374 146 1280
Fragments arranged by size:
4628 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2605 2605
Size: 7630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2314 2363 2705 2825 3062 3113
Size: 393 423 259 49 342 120 237 51
Cuts at: 3113 3123 3333 3437 4397 4577 4703 5693 6290
Size: 10 210 104 960 180 126 990 597
Cuts at: 6290 7077 7345 1239
Size: 787 268 1524
Fragments arranged by size:
1524 990 960 787 597 423 393 342
268 259 237 210 180 126 120 104
51 49 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2681 2681
Size: 7630
EcoO109I rG'GnC_Cy
Cuts at: 3413 3484 3586 4482 3413
Size: 71 102 896 6561
Fragments arranged by size:
6561 896 102 71
EcoRI G'AATT_C
Cuts at: 2902 4939 5317 2902
Size: 2037 378 5215
Fragments arranged by size:
5215 2037 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2234 4777 2234
Size: 2543 5087
FspI TGC'GCA
Cuts at: 6136 6136
Size: 7630
HaeII r_GCGC'y
Cuts at: 1678 6777 1678
Size: 5099 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 2501 2503 2533
Size: 32 135 32 680 32 217 2 30
Cuts at: 2533 2535 2594 2626 2784 2816 3617 3620 3649
Size: 2 59 32 158 32 801 3 29
Cuts at: 3649 3652 3995 4027 6271 6303 6345 6377 1373
Size: 3 343 32 2244 32 42 32 2626
Fragments arranged by size:
2626 2244 801 680 343 217 158 135
59 42 32 32 32 32 32 32
32 32 30 29 3 3 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2284 4371 4974 5220 669
Size: 988 281 346 2087 603 246 3079
Fragments arranged by size:
3079 2087 988 603 346 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7630
HpaI GTT'AAC
Cuts at: 5220 5220
Size: 7630
KpnI G_GTAC'C
Cuts at: 4955 4955
Size: 7630
MfeI C'AATT_G
Cuts at: 3887 4259 5229 3887
Size: 372 970 6288
Fragments arranged by size:
6288 970 372
MluI A'CGCG_T
Cuts at: 7506 7506
Size: 7630
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2169 4005 6744 6928 1305
Size: 864 1836 2739 184 2007
Fragments arranged by size:
2739 2007 1836 864 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2492 4166 10
Size: 54 1425 343 660 1674 3474
Fragments arranged by size:
3474 1674 1425 660 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2051 3210 3852 5588 5947 6106 518
Size: 648 885 1159 642 1736 359 159 2042
Fragments arranged by size:
2042 1736 1159 885 648 642 359 159
MspA1I CmG'CkG
Cuts at: 1606 2033 2075 2197 2524 3472 3991 4001 5046
Size: 427 42 122 327 948 519 10 1045
Cuts at: 5046 5723 6871 7116 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1045 948 677 519 427 327
245 122 42 10
NcoI C'CATG_G
Cuts at: 513 1065 4161 513
Size: 552 3096 3982
Fragments arranged by size:
3982 3096 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7630
NheI G'CTAG_C
Cuts at: 1052 4156 1052
Size: 3104 4526
NotI GC'GGCC_GC
Cuts at: 5077 5077
Size: 7630
NspI r_CATG'y
Cuts at: 1132 1229 3157 4231 4633 4786 4988 6533 1132
Size: 97 1928 1074 402 153 202 1545 2229
Fragments arranged by size:
2229 1928 1545 1074 402 202 153 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7630
PciI A'CATG_T
Cuts at: 4629 6529 4629
Size: 1900 5730
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7630
PfoI T'CCnGG_A
Cuts at: 2612 2802 2612
Size: 190 7440
PmeI GTTT'AAAC
Cuts at: 4934 4934
Size: 7630
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2850 2882 3969 4001 5699 5731 7212 7244 2850
Size: 32 1087 32 1698 32 1481 32 3236
Fragments arranged by size:
3236 1698 1481 1087 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3484 3586 4482 3484
Size: 102 896 6632
Fragments arranged by size:
6632 896 102
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7630
PsiI TTA'TAA
Cuts at: 2916 5200 2916
Size: 2284 5346
PstI C_TGCA'G
Cuts at: 830 2529 2829 3567 4982 830
Size: 1699 300 738 1415 3478
Fragments arranged by size:
3478 1699 1415 738 300
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7630
PvuII CAG'CTG
Cuts at: 2033 2075 2524 3472 3991 4001 2033
Size: 42 449 948 519 10 5662
Fragments arranged by size:
5662 948 519 449 42 10
SacI G_AGCT'C
Cuts at: 2607 2607
Size: 7630
SalI G'TCGA_C
Cuts at: 1936 4972 1936
Size: 3036 4594
SbfI CC_TGCA'GG
Cuts at: 4982 4982
Size: 7630
ScaI AGT'ACT
Cuts at: 3580 5878 7590 3580
Size: 2298 1712 3620
Fragments arranged by size:
3620 2298 1712
SexAI A'CCwGG_T
Cuts at: 3258 4362 3258
Size: 1104 6526
SfcI C'TryA_G
Cuts at: 826 1045 2525 2699 2825 3191 3563 4145 4400
Size: 219 1480 174 126 366 372 582 255
Cuts at: 4400 4978 6113 6794 6985 826
Size: 578 1135 681 191 1471
Fragments arranged by size:
1480 1471 1135 681 582 578 372 366
255 219 191 174 126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7630
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7630
SmaI CCC'GGG
Cuts at: 1872 4957 1872
Size: 3085 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2999 3902 4046 4105 5734 6635
Size: 197 926 1056 903 144 59 1629 901
Cuts at: 6635 6897 820
Size: 262 1553
Fragments arranged by size:
1629 1553 1056 926 903 901 262 197
144 59
SnaBI TAC'GTA
Cuts at: 493 5421 493
Size: 4928 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7630
SphI G_CATG'C
Cuts at: 1132 3157 4988 1132
Size: 2025 1831 3774
Fragments arranged by size:
3774 2025 1831
SspI AAT'ATT
Cuts at: 5 52 5554 5
Size: 47 5502 2081
Fragments arranged by size:
5502 2081 47
StuI AGG'CCT
Cuts at: 1517 4217 1517
Size: 2700 4930
StyI C'CwwG_G
Cuts at: 513 1065 4161 5069 513
Size: 552 3096 908 3074
Fragments arranged by size:
3096 3074 908 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2454 5657 5674 5827 6012 6457 325
Size: 1255 874 3203 17 153 185 445 1498
Fragments arranged by size:
3203 1498 1255 874 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3578 5876 7588
Size: 274 80 33 51 1443 1601 2298 1712
Cuts at: 7588 96
Size: 138
Fragments arranged by size:
2298 1712 1601 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7590 7622 7590
Size: 32 7598
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7630
XbaI T'CTAG_A
Cuts at: 4664 4966 4664
Size: 302 7328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2509 2509
Size: 7630
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7630
XmaI C'CCGG_G
Cuts at: 1870 4955 1870
Size: 3085 4545
XmnI GAAnn'nnTTC
Cuts at: 2672 3318 5759 2672
Size: 646 2441 4543
Fragments arranged by size:
4543 2441 646
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5438 276
Size: 53 83 186 936 3904 2468
Fragments arranged by size:
3904 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI