Restriction map for
pFN21AE3336
(Circular) (Six-base) MAPSORT of: pFN21AE3336.seq Check: 9025 from: 1 to: 7627
pFN21AE3336 7627 bp
With 182 enzymes: SgfI *
March 28, 2012 12:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2015 2770 4779 2015
Size: 755 2009 4863
Fragments arranged by size:
4863 2009 755
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5437 278
Size: 53 83 186 936 3901 2468
Fragments arranged by size:
3901 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2733 3294 4545 4970 1937
Size: 796 561 1251 425 4594
Fragments arranged by size:
4594 1251 796 561 425
Acc65I G'GTAC_C
Cuts at: 4948 4948
Size: 7627
AclI AA'CG_TT
Cuts at: 1563 5754 6127 1563
Size: 4191 373 3063
Fragments arranged by size:
4191 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2154 2458 2755 2768 3957 5690 7074 7342
Size: 522 304 297 13 1189 1733 1384 268
Cuts at: 7342 1632
Size: 1917
Fragments arranged by size:
1917 1733 1384 1189 522 304 297 268
13
AfeI AGC'GCT
Cuts at: 2372 2372
Size: 7627
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7430
AflIII A'CryG_T
Cuts at: 1203 2386 6526 7503 1203
Size: 1183 4140 977 1327
Fragments arranged by size:
4140 1327 1183 977
AgeI A'CCGG_T
Cuts at: 6458 6458
Size: 7627
AhdI GACnn_n'nnGTC
Cuts at: 6356 6356
Size: 7627
AleI CACnn'nnGTG
Cuts at: 1166 3752 1166
Size: 2586 5041
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4502 4727 4787 1184
Size: 3318 225 60 4024
Fragments arranged by size:
4024 3318 225 60
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 7595
AlwNI CAG_nnn'CTG
Cuts at: 3738 3955 4627 5370 6942 3738
Size: 217 672 743 1572 4423
Fragments arranged by size:
4423 1572 743 672 217
ApaI G_GGCC'C
Cuts at: 2850 3981 4837 4905 2850
Size: 1131 856 68 5572
Fragments arranged by size:
5572 1131 856 68
ApaLI G'TGCA_C
Cuts at: 2248 5684 6840 2248
Size: 3436 1156 3035
Fragments arranged by size:
3436 3035 1156
ApoI r'AATT_y
Cuts at: 1493 2105 4936 5162 5314 7423 1493
Size: 612 2831 226 152 2109 1697
Fragments arranged by size:
2831 2109 1697 612 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6181 160
Size: 872 5149 1606
Fragments arranged by size:
5149 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7627
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 3335 3480 3529 3852 4826 4847
Size: 73 95 1297 145 49 323 974 21
Cuts at: 4847 4952 1870
Size: 105 4545
Fragments arranged by size:
4545 1297 974 323 145 105 95 73
49 21
BamHI G'GATC_C
Cuts at: 4957 5324 4957
Size: 367 7260
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2058 2432 3367 3607
Size: 325 217 627 261 10 374 935 240
Cuts at: 3607 3617 4119 4921 4948 6403 618
Size: 10 502 802 27 1455 1842
Fragments arranged by size:
1842 1455 935 802 627 502 374 325
261 240 217 27 10 10
BanII G_rGCy'C
Cuts at: 1391 2096 2506 2850 3006 3354 3572 3587 3981
Size: 705 410 344 156 348 218 15 394
Cuts at: 3981 4837 4905 4946 1391
Size: 856 68 41 4072
Fragments arranged by size:
4072 856 705 410 394 348 344 218
156 68 41 15
BbeI G_GCGC'C
Cuts at: 4123 4123
Size: 7627
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2311 2352 928
Size: 971 412 41 6203
Fragments arranged by size:
6203 971 412 41
BbvCI CC'TCA_GC
Cuts at: 2624 3890 2624
Size: 1266 6361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3640 3674 5818 5852 1903
Size: 34 1703 34 2144 34 3678
Fragments arranged by size:
3678 2144 1703 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5519 6729 5519
Size: 1210 6417
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7627
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3010 6238 136
Size: 107 122 71 631 605 1338 3228 1525
Fragments arranged by size:
3228 1525 1338 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4575 7622 4575
Size: 3047 4580
BlpI GC'TnA_GC
Cuts at: 4886 5044 4886
Size: 158 7469
Bme1580I G_kGCm'C
Cuts at: 1790 2252 2850 3981 4837 4905 4926 5688 6844
Size: 462 598 1131 856 68 21 762 1156
Cuts at: 6844 1790
Size: 2573
Fragments arranged by size:
2573 1156 1131 856 762 598 462 68
21
BmgBI CAC'GTC
Cuts at: 1318 2644 3754 1318
Size: 1326 1110 5191
Fragments arranged by size:
5191 1326 1110
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2906 3224 3416
Size: 416 53 83 399 24 1489 318 192
Cuts at: 3416 3748 4609 5358 6316 442
Size: 332 861 749 958 1753
Fragments arranged by size:
1753 1489 958 861 749 416 399 332
318 192 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7627
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 3493 3586 4057 2593
Size: 900 93 471 6163
Fragments arranged by size:
6163 900 471 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2507 2965 3082 3368 3488 4023 4682
Size: 1010 258 458 117 286 120 535 659
Cuts at: 4682 6287 1239
Size: 1605 2579
Fragments arranged by size:
2579 1605 1010 659 535 458 286 258
120 117
Bpu10I CC'TnA_GC
Cuts at: 2624 3890 4628 2624
Size: 1266 738 5623
Fragments arranged by size:
5623 1266 738
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2320 3070 5752 6617 6915 2320
Size: 750 2682 865 298 3032
Fragments arranged by size:
3032 2682 865 750 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2195 3116 6290 882
Size: 617 696 921 3174 2219
Fragments arranged by size:
3174 2219 921 696 617
BsaAI yAC'GTr
Cuts at: 493 1204 5418 493
Size: 711 4214 2702
Fragments arranged by size:
4214 2702 711
BsaBI GATnn'nnATC
Cuts at: 7621 7621
Size: 7627
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4120 5434 5816 6451
Size: 53 83 186 936 2587 1314 382 635
Cuts at: 6451 275
Size: 1451
Fragments arranged by size:
2587 1451 1314 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6060 6458 6732 6879 7290 6060
Size: 398 274 147 411 6397
Fragments arranged by size:
6397 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2376 2406 3341 3371 3996
Size: 30 165 30 973 30 935 30 625
Cuts at: 3996 4026 1178
Size: 30 4779
Fragments arranged by size:
4779 973 935 625 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 2668 3014 3028 3416 3419 1186
Size: 952 530 346 14 388 3 5394
Fragments arranged by size:
5394 952 530 388 346 14 3
BseYI C'CCAG_C
Cuts at: 2317 3066 3134 4050 4395 4555 4890 6830 7326
Size: 749 68 916 345 160 335 1940 496
Cuts at: 7326 2317
Size: 2618
Fragments arranged by size:
2618 1940 916 749 496 345 335 160
68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2043 4314 4718 4811 1163
Size: 573 307 2271 404 93 3979
Fragments arranged by size:
3979 2271 573 404 307 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4289 4358 4874 5077 5838 6866
Size: 275 186 2288 69 516 203 761 1028
Cuts at: 6866 1540
Size: 2301
Fragments arranged by size:
2301 2288 1028 761 516 275 203 186
69
BsiHKAI G_wGCw'C
Cuts at: 2096 2252 3572 3587 5688 5773 6844 2096
Size: 156 1320 15 2101 85 1071 2879
Fragments arranged by size:
2879 2101 1320 1071 156 85 15
BsmI GAATG_Cn'
Cuts at: 2149 5138 5231 2149
Size: 2989 93 4545
Fragments arranged by size:
4545 2989 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2960 2960
Size: 7627
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2252 2506 2850 3006 3354 3572
Size: 399 306 156 254 344 156 348 218
Cuts at: 3572 3587 3981 4837 4905 4926 4946 5688 5773
Size: 15 394 856 68 21 20 742 85
Cuts at: 5773 6844 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 856 742 399 394 348 344
306 254 218 156 156 85 68 21
20 15
BspEI T'CCGG_A
Cuts at: 7290 7290
Size: 7627
BspHI T'CATG_A
Cuts at: 5516 7246 5516
Size: 1730 5897
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2015 2770 4779 4982 844
Size: 1171 755 2009 203 3489
Fragments arranged by size:
3489 2009 1171 755 203
BsrBI CCG'CTC
Cuts at: 1394 1823 4677 4831 4870 5514 1394
Size: 429 2854 154 39 644 3507
Fragments arranged by size:
3507 2854 644 429 154 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 3307 6122 6296 65
Size: 1183 2059 2815 174 1396
Fragments arranged by size:
2815 2059 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4590 4879 6271 6458 1700
Size: 110 2780 289 1392 187 2869
Fragments arranged by size:
2869 2780 1392 289 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7627
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7627
BssSI C'ACGA_G
Cuts at: 2186 2499 4865 5687 6699 2186
Size: 313 2366 822 1012 3114
Fragments arranged by size:
3114 2366 1012 822 313
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7627
BstBI TT'CG_AA
Cuts at: 2065 6431 2065
Size: 4366 3261
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7627
BstXI CCAn_nnnn'nTGG
Cuts at: 2989 3754 4210 2989
Size: 765 456 6406
Fragments arranged by size:
6406 765 456
BstYI r'GATC_y
Cuts at: 1972 2337 4575 4957 5324 5709 5726 7178 7264
Size: 365 2238 382 367 385 17 1452 86
Cuts at: 7264 7276 7622 1972
Size: 12 346 1977
Fragments arranged by size:
2238 1977 1452 385 382 367 365 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2851 3153 3522 4042 2851
Size: 302 369 520 6436
Fragments arranged by size:
6436 520 369 302
BtgI C'CryG_G
Cuts at: 513 1065 2004 2205 3020 3612 3705 4109 513
Size: 552 939 201 815 592 93 404 4031
Fragments arranged by size:
4031 939 815 592 552 404 201 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3426 487
Size: 683 56 2200 4688
Fragments arranged by size:
4688 2200 683 56
BtsI GCAGTG_nn'
Cuts at: 3341 4090 4302 5143 5937 5957 3341
Size: 749 212 841 794 20 5011
Fragments arranged by size:
5011 841 794 749 212 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7627
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4066 4101 529
Size: 35 3502 35 4055
Fragments arranged by size:
4055 3502 35 35
DraI TTT'AAA
Cuts at: 4931 4931
Size: 7627
DraIII CAC_nnn'GTG
Cuts at: 3367 3367
Size: 7627
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2912 4280 4541 6634 809
Size: 507 1596 1368 261 2093 1802
Fragments arranged by size:
2093 1802 1596 1368 507 261
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2652 2670
Size: 54 1425 50 275 18 177 645 18
Cuts at: 2670 2940 3228 4248 4286 4355 4653 4871 5074
Size: 270 288 1020 38 69 298 218 203
Cuts at: 5074 5963 7592 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1020 889 645 298 288 275
270 218 203 177 69 54 50 43
38 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 4286 4355 4871 5074 1537
Size: 275 2474 69 516 203 4090
Fragments arranged by size:
4090 2474 516 275 203 69
EarI CTCTTCn'nnn_
Cuts at: 1353 3206 3467 3728 5557 1353
Size: 1853 261 261 1829 3423
Fragments arranged by size:
3423 1853 1829 261 261
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6214 6588 6734 387
Size: 1064 138 4625 374 146 1280
Fragments arranged by size:
4625 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2094 3570 3585 2094
Size: 1476 15 6136
Fragments arranged by size:
6136 1476 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2249 2458 2507 2755 2768 2965
Size: 393 522 95 209 49 248 13 197
Cuts at: 2965 3082 3368 3488 3957 4023 4682 5690 6287
Size: 117 286 120 469 66 659 1008 597
Cuts at: 6287 7074 7342 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1008 787 659 597 522 469 393
286 268 248 209 197 120 117 95
66 49 13
EcoNI CCTnn'n_nnAGG
Cuts at: 3773 4487 4631 4715 3773
Size: 714 144 84 6685
Fragments arranged by size:
6685 714 144 84
EcoO109I rG'GnC_Cy
Cuts at: 2440 2809 2846 2847 3952 3978 4239 4902 2440
Size: 369 37 1 1105 26 261 663 5165
Fragments arranged by size:
5165 1105 663 369 261 37 26 1
EcoRI G'AATT_C
Cuts at: 2105 4936 5314 2105
Size: 2831 378 4418
Fragments arranged by size:
4418 2831 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4486 4741 4486
Size: 255 7372
FseI GG_CCGG'CC
Cuts at: 4594 4594
Size: 7627
FspI TGC'GCA
Cuts at: 3313 4478 4715 4775 6133 3313
Size: 1165 237 60 1358 4807
Fragments arranged by size:
4807 1358 1165 237 60
HaeII r_GCGC'y
Cuts at: 1678 2374 3544 3816 4123 4886 6774 1678
Size: 696 1170 272 307 763 1888 2531
Fragments arranged by size:
2531 1888 1170 763 696 307 272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3404 3436 6268 6300 6342
Size: 32 135 32 1832 32 2832 32 42
Cuts at: 6342 6374 1373
Size: 32 2626
Fragments arranged by size:
2832 2626 1832 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4971 5217 669
Size: 988 281 3033 246 3079
Fragments arranged by size:
3079 3033 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7627
HpaI GTT'AAC
Cuts at: 5217 5217
Size: 7627
KasI G'GCGC_C
Cuts at: 4119 4119
Size: 7627
KpnI G_GTAC'C
Cuts at: 4952 4952
Size: 7627
MfeI C'AATT_G
Cuts at: 5226 5226
Size: 7627
MluI A'CGCG_T
Cuts at: 7503 7503
Size: 7627
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2970 3213 4455 4833 6741 6925 1305
Size: 1665 243 1242 378 1908 184 2007
Fragments arranged by size:
2007 1908 1665 1242 378 243 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2672 2942 3230 10
Size: 54 1425 343 177 663 270 288 4407
Fragments arranged by size:
4407 1425 663 343 288 270 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 3752 4787 5585 5944 6103 518
Size: 648 993 1593 1035 798 359 159 2042
Fragments arranged by size:
2042 1593 1035 993 798 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3022 4050 4111 4375 4453 4876 4890 5043
Size: 1416 1028 61 264 78 423 14 153
Cuts at: 5043 5720 6868 7113 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1416 1148 1028 677 423 264 245
153 78 61 14
NaeI GCC'GGC
Cuts at: 4592 4881 4592
Size: 289 7338
NarI GG'CG_CC
Cuts at: 4120 4120
Size: 7627
NcoI C'CATG_G
Cuts at: 513 1065 2004 2205 513
Size: 552 939 201 5935
Fragments arranged by size:
5935 939 552 201
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7627
NgoMIV G'CCGG_C
Cuts at: 4590 4879 4590
Size: 289 7338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7627
NotI GC'GGCC_GC
Cuts at: 4871 5074 4871
Size: 203 7424
NspI r_CATG'y
Cuts at: 1132 1229 2390 4366 4985 6530 1132
Size: 97 1161 1976 619 1545 2229
Fragments arranged by size:
2229 1976 1545 1161 619 97
PasI CC'CwG_GG
Cuts at: 1348 2323 2521 4203 1348
Size: 975 198 1682 4772
Fragments arranged by size:
4772 1682 975 198
PciI A'CATG_T
Cuts at: 2386 6526 2386
Size: 4140 3487
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3642 3738 3955 1108
Size: 2534 96 217 4780
Fragments arranged by size:
4780 2534 217 96
PfoI T'CCnGG_A
Cuts at: 2265 2421 3465 2265
Size: 156 1044 6427
Fragments arranged by size:
6427 1044 156
PmeI GTTT'AAAC
Cuts at: 4931 4931
Size: 7627
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 5696 5728 7209 7241 3341
Size: 32 2323 32 1481 32 3727
Fragments arranged by size:
3727 2323 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2440 3952 4239 2440
Size: 1512 287 5828
Fragments arranged by size:
5828 1512 287
PshAI GACnn'nnGTC
Cuts at: 1613 2387 3881 1613
Size: 774 1494 5359
Fragments arranged by size:
5359 1494 774
PsiI TTA'TAA
Cuts at: 5197 5197
Size: 7627
PspOMI G'GGCC_C
Cuts at: 2846 3977 4833 4901 2846
Size: 1131 856 68 5572
Fragments arranged by size:
5572 1131 856 68
PspXI vC'TCGA_Gb
Cuts at: 3335 3529 3335
Size: 194 7433
PstI C_TGCA'G
Cuts at: 830 3287 3832 4979 830
Size: 2457 545 1147 3478
Fragments arranged by size:
3478 2457 1147 545
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7627
PvuII CAG'CTG
Cuts at: 4890 4890
Size: 7627
SacI G_AGCT'C
Cuts at: 2096 3572 3587 2096
Size: 1476 15 6136
Fragments arranged by size:
6136 1476 15
SacII CC_GC'GG
Cuts at: 3023 4112 3023
Size: 1089 6538
SalI G'TCGA_C
Cuts at: 1936 4969 1936
Size: 3033 4594
SanDI GG'GwC_CC
Cuts at: 2440 2440
Size: 7627
SbfI CC_TGCA'GG
Cuts at: 4979 4979
Size: 7627
ScaI AGT'ACT
Cuts at: 5875 7587 5875
Size: 1712 5915
SexAI A'CCwGG_T
Cuts at: 2727 3363 2727
Size: 636 6991
SfcI C'TryA_G
Cuts at: 826 1045 2602 2734 2836 3283 3295 3828 4546
Size: 219 1557 132 102 447 12 533 718
Cuts at: 4546 4975 6110 6791 6982 826
Size: 429 1135 681 191 1471
Fragments arranged by size:
1557 1471 1135 718 681 533 447 429
219 191 132 102 12
SfoI GGC'GCC
Cuts at: 4121 4121
Size: 7627
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7627
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7627
SmaI CCC'GGG
Cuts at: 1872 2040 3482 3854 4954 1872
Size: 168 1442 372 1100 4545
Fragments arranged by size:
4545 1442 1100 372 168
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2299 3085 3335 3529 5731 6632
Size: 197 926 356 786 250 194 2202 901
Cuts at: 6632 6894 820
Size: 262 1553
Fragments arranged by size:
2202 1553 926 901 786 356 262 250
197 194
SnaBI TAC'GTA
Cuts at: 493 5418 493
Size: 4925 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7627
SphI G_CATG'C
Cuts at: 1132 4985 1132
Size: 3853 3774
SspI AAT'ATT
Cuts at: 5 52 5551 5
Size: 47 5499 2081
Fragments arranged by size:
5499 2081 47
StuI AGG'CCT
Cuts at: 1517 3527 1517
Size: 2010 5617
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 2205 4278 5066 513
Size: 552 939 6 195 2073 788 3074
Fragments arranged by size:
3074 2073 939 788 552 195 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2915 3080 3464 3605 5654 5671 5824
Size: 1255 1335 165 384 141 2049 17 153
Cuts at: 5824 6009 6454 325
Size: 185 445 1498
Fragments arranged by size:
2049 1498 1335 1255 445 384 185 165
153 141 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3672 5873 7585
Size: 274 80 33 51 1443 1695 2201 1712
Cuts at: 7585 96
Size: 138
Fragments arranged by size:
2201 1712 1695 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2376 2408 3996 4028 7587 7619 2376
Size: 32 1588 32 3559 32 2384
Fragments arranged by size:
3559 2384 1588 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2642 1316
Size: 1326 6301
XbaI T'CTAG_A
Cuts at: 4963 4963
Size: 7627
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2125 2185 2212 3361 3754 2125
Size: 60 27 1149 393 5998
Fragments arranged by size:
5998 1149 393 60 27
XhoI C'TCGA_G
Cuts at: 1943 3335 3529 1943
Size: 1392 194 6041
Fragments arranged by size:
6041 1392 194
XmaI C'CCGG_G
Cuts at: 1870 2038 3480 3852 4952 1870
Size: 168 1442 372 1100 4545
Fragments arranged by size:
4545 1442 1100 372 168
XmnI GAAnn'nnTTC
Cuts at: 5756 5756
Size: 7627
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5435 276
Size: 53 83 186 936 3901 2468
Fragments arranged by size:
3901 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FspAI
NruI NsiI PacI PmlI PsrI RsrII SapI SfiI
SrfI SwaI