Restriction map for
pFN21AE3334
(Circular) (Six-base) MAPSORT of: pFN21AE3334.seq Check: 7776 from: 1 to: 7609
pFN21AE3334 7609 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2273 3430 2273
Size: 1157 6452
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5419 278
Size: 53 83 186 936 3883 2468
Fragments arranged by size:
3883 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2042 4952 1937
Size: 105 2910 4594
Fragments arranged by size:
4594 2910 105
Acc65I G'GTAC_C
Cuts at: 3500 4930 3500
Size: 1430 6179
AclI AA'CG_TT
Cuts at: 1563 3793 5736 6109 1563
Size: 2230 1943 373 3063
Fragments arranged by size:
3063 2230 1943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 2837 4409 4721 4754 5672 7056 7324
Size: 941 264 1572 312 33 918 1384 268
Cuts at: 7324 1632
Size: 1917
Fragments arranged by size:
1917 1572 1384 941 918 312 268 264
33
AfeI AGC'GCT
Cuts at: 4361 4361
Size: 7609
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7412
AflIII A'CryG_T
Cuts at: 1203 6508 7485 1203
Size: 5305 977 1327
Fragments arranged by size:
5305 1327 977
AgeI A'CCGG_T
Cuts at: 6440 6440
Size: 7609
AhdI GACnn_n'nnGTC
Cuts at: 2665 6338 2665
Size: 3673 3936
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3912 1184
Size: 2728 4881
AlwNI CAG_nnn'CTG
Cuts at: 2869 3224 3474 4651 4699 4732 5352 6924 2869
Size: 355 250 1177 48 33 620 1572 3554
Fragments arranged by size:
3554 1572 1177 620 355 250 48 33
ApaLI G'TGCA_C
Cuts at: 5666 6822 5666
Size: 1156 6453
ApoI r'AATT_y
Cuts at: 1493 3267 3599 4918 5144 5296 7405 1493
Size: 1774 332 1319 226 152 2109 1697
Fragments arranged by size:
2109 1774 1697 1319 332 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6163 160
Size: 872 5131 1606
Fragments arranged by size:
5131 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7609
AvaI C'yCGr_G
Cuts at: 1870 1943 2504 2606 3077 4934 1870
Size: 73 561 102 471 1857 4545
Fragments arranged by size:
4545 1857 561 471 102 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3484 3517 3484
Size: 33 7576
BamHI G'GATC_C
Cuts at: 3927 4939 5306 3927
Size: 1012 367 6230
Fragments arranged by size:
6230 1012 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 3103 3500 3690 4266
Size: 325 217 627 351 965 397 190 576
Cuts at: 4266 4930 6385 618
Size: 664 1455 1842
Fragments arranged by size:
1842 1455 965 664 627 576 397 351
325 217 190
BanII G_rGCy'C
Cuts at: 1391 2400 2724 3138 3545 4209 4928 1391
Size: 1009 324 414 407 664 719 4072
Fragments arranged by size:
4072 1009 719 664 414 407 324
BbeI G_GCGC'C
Cuts at: 2142 3694 2142
Size: 1552 6057
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2561 2967 928
Size: 971 662 406 5570
Fragments arranged by size:
5570 971 662 406
BbvCI CC'TCA_GC
Cuts at: 2928 3314 2928
Size: 386 7223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5800 5834 1903
Size: 34 3863 34 3678
Fragments arranged by size:
3863 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5501 6711 5501
Size: 1210 6399
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7609
BfrBI ATG'CAT
Cuts at: 3787 3787
Size: 7609
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6220 136
Size: 107 122 71 631 605 4548 1525
Fragments arranged by size:
4548 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2473 3332 4164 7604 2473
Size: 859 832 3440 2478
Fragments arranged by size:
3440 2478 859 832
BlpI GC'TnA_GC
Cuts at: 3297 4506 4826 5026 3297
Size: 1209 320 200 5880
Fragments arranged by size:
5880 1209 320 200
Bme1580I G_kGCm'C
Cuts at: 1790 3231 4847 5670 6826 1790
Size: 1441 1616 823 1156 2573
Fragments arranged by size:
2573 1616 1441 1156 823
BmgBI CAC'GTC
Cuts at: 1318 2080 1318
Size: 762 6847
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2847 3179 5340
Size: 416 53 83 399 24 1430 332 2161
Cuts at: 5340 6298 442
Size: 958 1753
Fragments arranged by size:
2161 1753 1430 958 416 399 332 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7609
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3559 3559
Size: 7609
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2393 2891 3035 3395 3420 3434 3704
Size: 1010 144 498 144 360 25 14 270
Cuts at: 3704 3797 3872 3956 4142 4292 6269 1239
Size: 93 75 84 186 150 1977 2579
Fragments arranged by size:
2579 1977 1010 498 360 270 186 150
144 144 93 84 75 25 14
Bpu10I CC'TnA_GC
Cuts at: 2928 3314 2928
Size: 386 7223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3569 5734 6599 6897 3569
Size: 2165 865 298 4281
Fragments arranged by size:
4281 2165 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2618 3371 4209 6272 882
Size: 617 1119 753 838 2063 2219
Fragments arranged by size:
2219 2063 1119 838 753 617
BsaAI yAC'GTr
Cuts at: 493 1204 5400 493
Size: 711 4196 2702
Fragments arranged by size:
4196 2702 711
BsaBI GATnn'nnATC
Cuts at: 2252 7603 2252
Size: 5351 2258
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2139 2361 2991 3691
Size: 53 83 186 936 606 222 630 700
Cuts at: 3691 5416 5798 6433 275
Size: 1725 382 635 1451
Fragments arranged by size:
1725 1451 936 700 635 630 606 382
222 186 83 53
BsaWI w'CCGG_w
Cuts at: 6042 6440 6714 6861 7272 6042
Size: 398 274 147 411 6379
Fragments arranged by size:
6379 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4169 4199 4826 4856 1178
Size: 30 165 30 2766 30 627 30 3931
Fragments arranged by size:
3931 2766 627 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2234 2237 2999 3025 3095 3686 3929 4073
Size: 1048 3 762 26 70 591 243 144
Cuts at: 4073 4450 4559 4847 1186
Size: 377 109 288 3948
Fragments arranged by size:
3948 1048 762 591 377 288 243 144
109 70 26 3
BseYI C'CCAG_C
Cuts at: 2128 3107 3319 3747 4536 6812 7308 2128
Size: 979 212 428 789 2276 496 2429
Fragments arranged by size:
2429 2276 979 789 496 428 212
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 3460 1163
Size: 573 696 1028 5312
Fragments arranged by size:
5312 1028 696 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2968 4760 5059 5820 6848 1540
Size: 275 186 967 1792 299 761 1028 2301
Fragments arranged by size:
2301 1792 1028 967 761 299 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2724 4065 4209 4251 4686 5670 5755 6826
Size: 645 1341 144 42 435 984 85 1071
Cuts at: 6826 2079
Size: 2862
Fragments arranged by size:
2862 1341 1071 984 645 435 144 85
42
BsmI GAATG_Cn'
Cuts at: 5120 5213 5120
Size: 93 7516
BsmBI CGTCTCn'nnnn_
Cuts at: 2168 2168
Size: 7609
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2400 2724 3138 3231 3545 4065
Size: 399 289 321 324 414 93 314 520
Cuts at: 4065 4209 4251 4686 4847 4928 5670 5755 6826
Size: 144 42 435 161 81 742 85 1071
Cuts at: 6826 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 520 435 414 399 324
321 314 289 161 144 93 85 81
42
BspEI T'CCGG_A
Cuts at: 7272 7272
Size: 7609
BspHI T'CATG_A
Cuts at: 2328 4191 5498 7228 2328
Size: 1863 1307 1730 2709
Fragments arranged by size:
2709 1863 1730 1307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2273 3430 4964 844
Size: 1429 1157 1534 3489
Fragments arranged by size:
3489 1534 1429 1157
BsrBI CCG'CTC
Cuts at: 1394 1823 4345 4567 5496 1394
Size: 429 2522 222 929 3507
Fragments arranged by size:
3507 2522 929 429 222
BsrDI GCAATG_nn'
Cuts at: 65 1248 6104 6278 65
Size: 1183 4856 174 1396
Fragments arranged by size:
4856 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3354 6253 6440 1700
Size: 110 1544 2899 187 2869
Fragments arranged by size:
2899 2869 1544 187 110
BsrGI T'GTAC_A
Cuts at: 96 3570 4464 96
Size: 3474 894 3241
Fragments arranged by size:
3474 3241 894
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7609
BssSI C'ACGA_G
Cuts at: 2717 4316 4589 5669 6681 2717
Size: 1599 273 1080 1012 3645
Fragments arranged by size:
3645 1599 1080 1012 273
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3474 3769 4255 4321 4651 794
Size: 2680 295 486 66 330 3752
Fragments arranged by size:
3752 2680 486 330 295 66
BstBI TT'CG_AA
Cuts at: 6413 6413
Size: 7609
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7609
BstXI CCAn_nnnn'nTGG
Cuts at: 2371 3100 3152 2371
Size: 729 52 6828
Fragments arranged by size:
6828 729 52
BstYI r'GATC_y
Cuts at: 1972 2253 2473 3161 3332 3927 4164 4428 4783
Size: 281 220 688 171 595 237 264 355
Cuts at: 4783 4939 5306 5691 5708 7160 7246 7258 7604
Size: 156 367 385 17 1452 86 12 346
Cuts at: 7604 1972
Size: 1977
Fragments arranged by size:
1977 1452 688 595 385 367 355 346
281 264 237 220 171 156 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 2994 513
Size: 552 939 351 639 5128
Fragments arranged by size:
5128 939 639 552 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6870
Fragments arranged by size:
6870 683 56
BtsI GCAGTG_nn'
Cuts at: 3225 4625 5125 5919 5939 3225
Size: 1400 500 794 20 4895
Fragments arranged by size:
4895 1400 794 500 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7609
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7574
DraI TTT'AAA
Cuts at: 4913 4913
Size: 7609
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6616 809
Size: 507 5300 1802
Fragments arranged by size:
5300 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2259 2965
Size: 54 1425 50 275 18 363 66 706
Cuts at: 2965 3399 4128 4458 4757 5056 5945 7574 8
Size: 434 729 330 299 299 889 1629 43
Fragments arranged by size:
1629 1425 889 729 706 434 363 330
299 299 275 66 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2965 4757 5056 1537
Size: 275 1153 1792 299 4090
Fragments arranged by size:
4090 1792 1153 299 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 2946 2976 3723 4130 4239 4466 4544
Size: 695 898 30 747 407 109 227 78
Cuts at: 4544 4773 5539 1353
Size: 229 766 3423
Fragments arranged by size:
3423 898 766 747 695 407 229 227
109 78 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2012 2072 3039 3920 3940 4853
Size: 1064 138 423 60 967 881 20 913
Cuts at: 4853 6196 6570 6716 387
Size: 1343 374 146 1280
Fragments arranged by size:
1343 1280 1064 967 913 881 423 374
146 138 60 20
EcoICRI GAG'CTC
Cuts at: 2722 4207 2722
Size: 1485 6124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2393 2573 2837 2891 3035 3395
Size: 393 617 144 180 264 54 144 360
Cuts at: 3395 3420 3434 3704 3797 3872 3956 4142 4292
Size: 25 14 270 93 75 84 186 150
Cuts at: 4292 4409 4721 4754 5672 6269 7056 7324 1239
Size: 117 312 33 918 597 787 268 1524
Fragments arranged by size:
1524 918 787 617 597 393 360 312
270 268 264 186 180 150 144 144
117 93 84 75 54 33 25 14
EcoNI CCTnn'n_nnAGG
Cuts at: 3938 3938
Size: 7609
EcoO109I rG'GnC_Cy
Cuts at: 2737 3310 3440 3559 4117 4705 2737
Size: 573 130 119 558 588 5641
Fragments arranged by size:
5641 588 573 558 130 119
EcoRI G'AATT_C
Cuts at: 3267 4918 5296 3267
Size: 1651 378 5580
Fragments arranged by size:
5580 1651 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3844 3844
Size: 7609
FspI TGC'GCA
Cuts at: 4328 6115 4328
Size: 1787 5822
HaeII r_GCGC'y
Cuts at: 1678 2142 3372 3694 4363 6756 1678
Size: 464 1230 322 669 2393 2531
Fragments arranged by size:
2531 2393 1230 669 464 322
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2651 2683 2990 3022 3529
Size: 32 135 32 1079 32 307 32 507
Cuts at: 3529 3561 6250 6282 6324 6356 1373
Size: 32 2689 32 42 32 2626
Fragments arranged by size:
2689 2626 1079 507 307 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4953 5199 669
Size: 988 281 3015 246 3079
Fragments arranged by size:
3079 3015 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4751 748
Size: 4003 3606
HpaI GTT'AAC
Cuts at: 5199 5199
Size: 7609
KasI G'GCGC_C
Cuts at: 2138 3690 2138
Size: 1552 6057
KpnI G_GTAC'C
Cuts at: 3504 4934 3504
Size: 1430 6179
MfeI C'AATT_G
Cuts at: 5208 5208
Size: 7609
MluI A'CGCG_T
Cuts at: 7485 7485
Size: 7609
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2739 4542 6723 6907 1305
Size: 1434 1803 2181 184 2007
Fragments arranged by size:
2181 2007 1803 1434 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 3401 4130 4460 10
Size: 54 1425 343 429 1140 729 330 3159
Fragments arranged by size:
3159 1425 1140 729 429 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4460 5567 5926 6085 518
Size: 648 3294 1107 359 159 2042
Fragments arranged by size:
3294 2042 1107 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2866 3100 3751 3775 4030 4255 4273 4312
Size: 1260 234 651 24 255 225 18 39
Cuts at: 4312 4387 4505 4651 5025 5702 6850 7095 1606
Size: 75 118 146 374 677 1148 245 2120
Fragments arranged by size:
2120 1260 1148 677 651 374 255 245
234 225 146 118 75 39 24 18
NaeI GCC'GGC
Cuts at: 3356 3356
Size: 7609
NarI GG'CG_CC
Cuts at: 2139 3691 2139
Size: 1552 6057
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6118
Fragments arranged by size:
6118 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7609
NgoMIV G'CCGG_C
Cuts at: 3354 3354
Size: 7609
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7609
NotI GC'GGCC_GC
Cuts at: 5056 5056
Size: 7609
NsiI A_TGCA'T
Cuts at: 3789 3789
Size: 7609
NspI r_CATG'y
Cuts at: 1132 1229 4612 4967 6512 1132
Size: 97 3383 355 1545 2229
Fragments arranged by size:
3383 2229 1545 355 97
PasI CC'CwG_GG
Cuts at: 1348 2671 1348
Size: 1323 6286
PciI A'CATG_T
Cuts at: 6508 6508
Size: 7609
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4675 1108
Size: 3567 4042
PmeI GTTT'AAAC
Cuts at: 4913 4913
Size: 7609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5678 5710 7191 7223 5678
Size: 32 1481 32 6064
Fragments arranged by size:
6064 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2737 3310 3559 4117 2737
Size: 573 249 558 6229
Fragments arranged by size:
6229 573 558 249
PshAI GACnn'nnGTC
Cuts at: 1613 3250 1613
Size: 1637 5972
PsiI TTA'TAA
Cuts at: 5179 5179
Size: 7609
PspXI vC'TCGA_Gb
Cuts at: 2504 2504
Size: 7609
PstI C_TGCA'G
Cuts at: 830 3143 3467 3774 4260 4575 4647 4656 4961
Size: 2313 324 307 486 315 72 9 305
Cuts at: 4961 830
Size: 3478
Fragments arranged by size:
3478 2313 486 324 315 307 305 72
9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7609
PvuII CAG'CTG
Cuts at: 2866 3751 3775 4255 4273 4387 4505 4651 2866
Size: 885 24 480 18 114 118 146 5824
Fragments arranged by size:
5824 885 480 146 118 114 24 18
SacI G_AGCT'C
Cuts at: 2724 4209 2724
Size: 1485 6124
SalI G'TCGA_C
Cuts at: 1936 4951 1936
Size: 3015 4594
SapI GCTCTTCn'nnn_
Cuts at: 3723 4239 4466 3723
Size: 516 227 6866
Fragments arranged by size:
6866 516 227
SbfI CC_TGCA'GG
Cuts at: 4961 4961
Size: 7609
ScaI AGT'ACT
Cuts at: 4301 5857 7569 4301
Size: 1556 1712 4341
Fragments arranged by size:
4341 1712 1556
SexAI A'CCwGG_T
Cuts at: 3956 3956
Size: 7609
SfcI C'TryA_G
Cuts at: 826 1045 2561 2796 3139 3463 3770 4256 4571
Size: 219 1516 235 343 324 307 486 315
Cuts at: 4571 4643 4652 4888 4957 6092 6773 6964 826
Size: 72 9 236 69 1135 681 191 1471
Fragments arranged by size:
1516 1471 1135 681 486 343 324 315
307 236 235 219 191 72 69 9
SfoI GGC'GCC
Cuts at: 2140 3692 2140
Size: 1552 6057
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7609
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7609
SmaI CCC'GGG
Cuts at: 1872 4936 1872
Size: 3064 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2504 3077 3584 5713 6614 6876
Size: 197 926 561 573 507 2129 901 262
Cuts at: 6876 820
Size: 1553
Fragments arranged by size:
2129 1553 926 901 573 561 507 262
197
SnaBI TAC'GTA
Cuts at: 493 5400 493
Size: 4907 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7609
SphI G_CATG'C
Cuts at: 1132 4967 1132
Size: 3835 3774
SspI AAT'ATT
Cuts at: 5 52 5533 5
Size: 47 5481 2081
Fragments arranged by size:
5481 2081 47
StuI AGG'CCT
Cuts at: 1517 2388 1517
Size: 871 6738
StyI C'CwwG_G
Cuts at: 513 1065 2004 2934 3381 5048 513
Size: 552 939 930 447 1667 3074
Fragments arranged by size:
3074 1667 939 930 552 447
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3121 3374 5636 5653 5806 5991 6436
Size: 1255 1541 253 2262 17 153 185 445
Cuts at: 6436 325
Size: 1498
Fragments arranged by size:
2262 1541 1498 1255 445 253 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3570 4299 4464
Size: 274 80 33 51 1443 1593 729 165
Cuts at: 4464 5855 7567 96
Size: 1391 1712 138
Fragments arranged by size:
1712 1593 1443 1391 729 274 165 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4169 4201 4826 4858 7569 7601 4169
Size: 32 625 32 2711 32 4177
Fragments arranged by size:
4177 2711 625 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7609
XbaI T'CTAG_A
Cuts at: 4945 4945
Size: 7609
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3409 3409
Size: 7609
XhoI C'TCGA_G
Cuts at: 1943 2504 3077 1943
Size: 561 573 6475
Fragments arranged by size:
6475 573 561
XmaI C'CCGG_G
Cuts at: 1870 4934 1870
Size: 3064 4545
XmnI GAAnn'nnTTC
Cuts at: 3824 5738 3824
Size: 1914 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5417 276
Size: 53 83 186 936 3883 2468
Fragments arranged by size:
3883 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BsiWI BstZ17I Bsu36I DraIII
EcoRV FseI FspAI NruI PacI PfoI PmlI PspOMI
PsrI RsrII SacII SanDI SfiI SrfI SwaI