Restriction map for
pFN21AE3323
(Circular) (Six-base) MAPSORT of: pFN21AE3323.seq Check: 9714 from: 1 to: 7456
pFN21AE3323 7456 bp
With 182 enzymes: SgfI *
March 28, 2012 12:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5266 278
Size: 53 83 186 936 3730 2468
Fragments arranged by size:
3730 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3622 4197 4799 1937
Size: 1685 575 602 4594
Fragments arranged by size:
4594 1685 602 575
Acc65I G'GTAC_C
Cuts at: 4777 4777
Size: 7456
AclI AA'CG_TT
Cuts at: 1563 5583 5956 1563
Size: 4020 373 3063
Fragments arranged by size:
4020 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2291 3884 4146 4314 4340 5519 6903 7171
Size: 659 1593 262 168 26 1179 1384 268
Cuts at: 7171 1632
Size: 1917
Fragments arranged by size:
1917 1593 1384 1179 659 268 262 168
26
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7259
AflIII A'CryG_T
Cuts at: 1203 2503 6355 7332 1203
Size: 1300 3852 977 1327
Fragments arranged by size:
3852 1327 1300 977
AgeI A'CCGG_T
Cuts at: 3641 6287 3641
Size: 2646 4810
AhdI GACnn_n'nnGTC
Cuts at: 6185 6185
Size: 7456
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7456
AlwNI CAG_nnn'CTG
Cuts at: 5199 6771 5199
Size: 1572 5884
ApaI G_GGCC'C
Cuts at: 2135 2155 2180 2135
Size: 20 25 7411
Fragments arranged by size:
7411 25 20
ApaLI G'TGCA_C
Cuts at: 5513 6669 5513
Size: 1156 6300
ApoI r'AATT_y
Cuts at: 1493 2658 3329 3732 4288 4444 4730 4765 4991
Size: 1165 671 403 556 156 286 35 226
Cuts at: 4991 5143 7252 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1165 671 556 403 286 226
156 152 35
AseI AT'TA_AT
Cuts at: 160 1032 2967 3399 4145 6010 160
Size: 872 1935 432 746 1865 1606
Fragments arranged by size:
1935 1865 1606 872 746 432
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7456
AvaI C'yCGr_G
Cuts at: 1870 1943 2179 2209 2216 2310 2709 4781 1870
Size: 73 236 30 7 94 399 2072 4545
Fragments arranged by size:
4545 2072 399 236 94 73 30 7
BamHI G'GATC_C
Cuts at: 4786 5153 4786
Size: 367 7089
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2305 3388 3526 4777 6232
Size: 325 217 627 518 1083 138 1251 1455
Cuts at: 6232 618
Size: 1842
Fragments arranged by size:
1842 1455 1251 1083 627 518 325 217
138
BanII G_rGCy'C
Cuts at: 1391 2135 2155 2180 2259 2931 4775 1391
Size: 744 20 25 79 672 1844 4072
Fragments arranged by size:
4072 1844 744 672 79 25 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3294 4320 928
Size: 971 1395 1026 4064
Fragments arranged by size:
4064 1395 1026 971
BbvCI CC'TCA_GC
Cuts at: 2223 2865 2223
Size: 642 6814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5647 5681 1903
Size: 34 3710 34 3678
Fragments arranged by size:
3710 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2506 4579 5348 6558 2506
Size: 2073 769 1210 3404
Fragments arranged by size:
3404 2073 1210 769
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7456
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2139 6067 136
Size: 107 122 71 631 605 467 3928 1525
Fragments arranged by size:
3928 1525 631 605 467 122 107 71
BglII A'GATC_T
Cuts at: 2428 7451 2428
Size: 5023 2433
BlpI GC'TnA_GC
Cuts at: 2324 2930 4873 2324
Size: 606 1943 4907
Fragments arranged by size:
4907 1943 606
Bme1580I G_kGCm'C
Cuts at: 1790 2117 2135 2155 2180 2310 3562 5517 6673
Size: 327 18 20 25 130 1252 1955 1156
Cuts at: 6673 1790
Size: 2573
Fragments arranged by size:
2573 1955 1252 1156 327 130 25 20
18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7456
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3135 5187 6145
Size: 416 53 83 399 24 1718 2052 958
Cuts at: 6145 442
Size: 1753
Fragments arranged by size:
2052 1753 1718 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2931 6116 1239
Size: 1692 3185 2579
Fragments arranged by size:
3185 2579 1692
Bpu10I CC'TnA_GC
Cuts at: 2223 2865 2223
Size: 642 6814
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2393 3389 3944 5581 6446 6744 2393
Size: 996 555 1637 865 298 3105
Fragments arranged by size:
3105 1637 996 865 555 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6119 882
Size: 617 4620 2219
Fragments arranged by size:
4620 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5247 493
Size: 711 4043 2702
Fragments arranged by size:
4043 2702 711
BsaBI GATnn'nnATC
Cuts at: 2517 7450 2517
Size: 4933 2523
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5263 5645 6280 275
Size: 53 83 186 936 3730 382 635 1451
Fragments arranged by size:
3730 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3641 5889 6287 6561 6708 7119 3641
Size: 2248 398 274 147 411 3978
Fragments arranged by size:
3978 2248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3601 3631 3888 3901 3918
Size: 30 165 30 2198 30 257 13 17
Cuts at: 3918 3931 1178
Size: 13 4703
Fragments arranged by size:
4703 2198 257 165 30 30 30 17
13 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2354 2721 3896 3909 1186
Size: 907 261 367 1175 13 4733
Fragments arranged by size:
4733 1175 907 367 261 13
BseYI C'CCAG_C
Cuts at: 2173 6659 7155 2173
Size: 4486 496 2474
Fragments arranged by size:
4486 2474 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6883
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3556 4906 5667 6695 1540
Size: 275 186 1555 1350 761 1028 2301
Fragments arranged by size:
2301 1555 1350 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2931 5517 5602 6673 2931
Size: 2586 85 1071 3714
Fragments arranged by size:
3714 2586 1071 85
BsmI GAATG_Cn'
Cuts at: 3060 4177 4967 5060 3060
Size: 1117 790 93 5456
Fragments arranged by size:
5456 1117 790 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2135 2155 2180 2259 2310 2931
Size: 399 327 18 20 25 79 51 621
Cuts at: 2931 3562 4775 5517 5602 6673 1391
Size: 631 1213 742 85 1071 2174
Fragments arranged by size:
2174 1213 1071 742 631 621 399 327
85 79 51 25 20 18
BspEI T'CCGG_A
Cuts at: 7119 7119
Size: 7456
BspHI T'CATG_A
Cuts at: 2734 4515 5345 7075 2734
Size: 1781 830 1730 3115
Fragments arranged by size:
3115 1781 1730 830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4811 844
Size: 3967 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2065 3598 5343 1394
Size: 429 242 1533 1745 3507
Fragments arranged by size:
3507 1745 1533 429 242
BsrDI GCAATG_nn'
Cuts at: 65 1248 3588 5951 6125 65
Size: 1183 2340 2363 174 1396
Fragments arranged by size:
2363 2340 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3641 6100 6287 1700
Size: 110 1831 2459 187 2869
Fragments arranged by size:
2869 2459 1831 187 110
BsrGI T'GTAC_A
Cuts at: 96 3429 4573 96
Size: 3333 1144 2979
Fragments arranged by size:
3333 2979 1144
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7456
BssSI C'ACGA_G
Cuts at: 5516 6528 5516
Size: 1012 6444
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3343 794
Size: 2549 4907
BstBI TT'CG_AA
Cuts at: 6260 6260
Size: 7456
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7456
BstYI r'GATC_y
Cuts at: 1972 2428 4786 5153 5538 5555 7007 7093 7105
Size: 456 2358 367 385 17 1452 86 12
Cuts at: 7105 7451 1972
Size: 346 1977
Fragments arranged by size:
2358 1977 1452 456 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 4198 4198
Size: 7456
Bsu36I CC'TnA_GG
Cuts at: 2976 2976
Size: 7456
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6904
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2088 487
Size: 683 56 862 5855
Fragments arranged by size:
5855 862 683 56
BtsI GCAGTG_nn'
Cuts at: 4972 5766 5786 4972
Size: 794 20 6642
Fragments arranged by size:
6642 794 20
ClaI AT'CG_AT
Cuts at: 1554 4420 1554
Size: 2866 4590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4107 4142 529
Size: 35 3543 35 3843
Fragments arranged by size:
3843 3543 35 35
DraI TTT'AAA
Cuts at: 4760 4760
Size: 7456
DraIII CAC_nnn'GTG
Cuts at: 4723 4723
Size: 7456
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6463 809
Size: 507 5147 1802
Fragments arranged by size:
5147 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4903 5792 7421
Size: 54 1425 50 275 18 3073 889 1629
Cuts at: 7421 8
Size: 43
Fragments arranged by size:
3073 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4903 1537
Size: 275 3091 4090
Fragments arranged by size:
4090 3091 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 2055 2118 2919 5386 1353
Size: 696 6 63 801 2467 3423
Fragments arranged by size:
3423 2467 801 696 63 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6043 6417 6563 387
Size: 1064 138 4454 374 146 1280
Fragments arranged by size:
4454 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2929 2929
Size: 7456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2931 3884 4146 4314 4340 5519
Size: 393 659 640 953 262 168 26 1179
Cuts at: 5519 6116 6903 7171 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1179 953 787 659 640 597 393
268 262 168 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2751 2751
Size: 7456
EcoO109I rG'GnC_Cy
Cuts at: 2132 2151 2152 4093 2132
Size: 19 1 1941 5495
Fragments arranged by size:
5495 1941 19 1
EcoRI G'AATT_C
Cuts at: 4444 4765 5143 4444
Size: 321 378 6757
Fragments arranged by size:
6757 378 321
EcoRV GAT'ATC
Cuts at: 4157 4157
Size: 7456
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2447 2777 3383 3785 2447
Size: 330 606 402 6118
Fragments arranged by size:
6118 606 402 330
FspI TGC'GCA
Cuts at: 2036 5962 2036
Size: 3926 3530
HaeII r_GCGC'y
Cuts at: 1678 2701 6603 1678
Size: 1023 3902 2531
Fragments arranged by size:
3902 2531 1023
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2523 2555 3086 3118 3339
Size: 32 135 32 951 32 531 32 221
Cuts at: 3339 3371 3601 3633 3640 3672 3888 3920 4413
Size: 32 230 32 7 32 216 32 493
Cuts at: 4413 4445 4528 4560 6097 6129 6171 6203 1373
Size: 32 83 32 1537 32 42 32 2626
Fragments arranged by size:
2626 1537 951 531 493 230 221 216
135 83 42 32 32 32 32 32
32 32 32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2993 4800 5046 669
Size: 988 281 1055 1807 246 3079
Fragments arranged by size:
3079 1807 1055 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7456
HpaI GTT'AAC
Cuts at: 5046 5046
Size: 7456
KpnI G_GTAC'C
Cuts at: 4781 4781
Size: 7456
MfeI C'AATT_G
Cuts at: 3786 4492 5055 3786
Size: 706 563 6187
Fragments arranged by size:
6187 706 563
MluI A'CGCG_T
Cuts at: 7332 7332
Size: 7456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3180 6570 6754 1305
Size: 1875 3390 184 2007
Fragments arranged by size:
3390 2007 1875 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5634
Fragments arranged by size:
5634 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2739 5414 5773 5932 518
Size: 648 1573 2675 359 159 2042
Fragments arranged by size:
2675 2042 1573 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2471 3427 4872 5549 6697 6942 1606
Size: 865 956 1445 677 1148 245 2120
Fragments arranged by size:
2120 1445 1148 956 865 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6904
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7456
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7456
NotI GC'GGCC_GC
Cuts at: 4903 4903
Size: 7456
NruI TCG'CGA
Cuts at: 2292 2292
Size: 7456
NspI r_CATG'y
Cuts at: 1132 1229 2507 2684 3578 4814 6359 1132
Size: 97 1278 177 894 1236 1545 2229
Fragments arranged by size:
2229 1545 1278 1236 894 177 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7456
PciI A'CATG_T
Cuts at: 2503 6355 2503
Size: 3852 3604
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2603 4509 1108
Size: 1495 1906 4055
Fragments arranged by size:
4055 1906 1495
PfoI T'CCnGG_A
Cuts at: 2331 2650 2331
Size: 319 7137
PmeI GTTT'AAAC
Cuts at: 4760 4760
Size: 7456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5525 5557 7038 7070 5525
Size: 32 1481 32 5911
Fragments arranged by size:
5911 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4093 4093
Size: 7456
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7456
PsiI TTA'TAA
Cuts at: 5026 5026
Size: 7456
PspOMI G'GGCC_C
Cuts at: 2131 2151 2176 2131
Size: 20 25 7411
Fragments arranged by size:
7411 25 20
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4597 4629 4597
Size: 32 7424
PstI C_TGCA'G
Cuts at: 830 4808 830
Size: 3978 3478
PvuI CG_AT'CG
Cuts at: 2001 3556 2001
Size: 1555 5901
PvuII CAG'CTG
Cuts at: 2471 3427 2471
Size: 956 6500
SacI G_AGCT'C
Cuts at: 2931 2931
Size: 7456
SalI G'TCGA_C
Cuts at: 1936 4798 1936
Size: 2862 4594
SanDI GG'GwC_CC
Cuts at: 4093 4093
Size: 7456
SapI GCTCTTCn'nnn_
Cuts at: 2055 2919 2055
Size: 864 6592
SbfI CC_TGCA'GG
Cuts at: 4808 4808
Size: 7456
ScaI AGT'ACT
Cuts at: 5704 7416 5704
Size: 1712 5744
SfcI C'TryA_G
Cuts at: 826 1045 3696 4804 5939 6620 6811 826
Size: 219 2651 1108 1135 681 191 1471
Fragments arranged by size:
2651 1471 1135 1108 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7456
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7456
SmaI CCC'GGG
Cuts at: 1872 2181 2211 2312 4783 1872
Size: 309 30 101 2471 4545
Fragments arranged by size:
4545 2471 309 101 30
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 3368 3923 5560 6461 6723
Size: 197 926 465 960 555 1637 901 262
Cuts at: 6723 820
Size: 1553
Fragments arranged by size:
1637 1553 960 926 901 555 465 262
197
SnaBI TAC'GTA
Cuts at: 493 5247 493
Size: 4754 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7456
SphI G_CATG'C
Cuts at: 1132 3578 4814 1132
Size: 2446 1236 3774
Fragments arranged by size:
3774 2446 1236
SspI AAT'ATT
Cuts at: 5 52 5380 5
Size: 47 5328 2081
Fragments arranged by size:
5328 2081 47
StuI AGG'CCT
Cuts at: 1517 2287 3669 1517
Size: 770 1382 5304
Fragments arranged by size:
5304 1382 770
StyI C'CwwG_G
Cuts at: 513 1065 4895 513
Size: 552 3830 3074
Fragments arranged by size:
3830 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5483 5500 5653 5838 6283 325
Size: 1255 3903 17 153 185 445 1498
Fragments arranged by size:
3903 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3012 3429 4573
Size: 274 80 33 51 1443 1035 417 1144
Cuts at: 4573 5702 7414 96
Size: 1129 1712 138
Fragments arranged by size:
1712 1443 1144 1129 1035 417 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3901 3933 7416 7448 3901
Size: 32 3483 32 3909
Fragments arranged by size:
3909 3483 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7456
XbaI T'CTAG_A
Cuts at: 4792 4792
Size: 7456
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7456
XmaI C'CCGG_G
Cuts at: 1870 2179 2209 2310 4781 1870
Size: 309 30 101 2471 4545
Fragments arranged by size:
4545 2471 309 101 30
XmnI GAAnn'nnTTC
Cuts at: 5585 5585
Size: 7456
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5264 276
Size: 53 83 186 936 3730 2468
Fragments arranged by size:
3730 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI FseI FspAI KasI NaeI
NarI NgoMIV NsiI PacI PmlI PspXI RsrII SacII
SexAI SfiI SfoI SrfI SwaI XcmI