Restriction map for
pFN21AE3322
(Circular) (Six-base) MAPSORT of: pFN21AE3322.seq Check: 3505 from: 1 to: 7444
pFN21AE3322 7444 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2223 2223
Size: 7444
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5254 278
Size: 53 83 186 936 3718 2468
Fragments arranged by size:
3718 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3446 3927 4787 1937
Size: 1509 481 860 4594
Fragments arranged by size:
4594 1509 860 481
Acc65I G'GTAC_C
Cuts at: 4765 4765
Size: 7444
AclI AA'CG_TT
Cuts at: 1563 5571 5944 1563
Size: 4008 373 3063
Fragments arranged by size:
4008 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2453 4718 5507 6891 7159 1632
Size: 821 2265 789 1384 268 1917
Fragments arranged by size:
2265 1917 1384 821 789 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7247
AflIII A'CryG_T
Cuts at: 1203 6343 7320 1203
Size: 5140 977 1327
Fragments arranged by size:
5140 1327 977
AgeI A'CCGG_T
Cuts at: 6275 6275
Size: 7444
AhdI GACnn_n'nnGTC
Cuts at: 6173 6173
Size: 7444
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3357 1184
Size: 2173 5271
AlwNI CAG_nnn'CTG
Cuts at: 2276 3116 4270 5187 6759 2276
Size: 840 1154 917 1572 2961
Fragments arranged by size:
2961 1572 1154 917 840
ApaLI G'TGCA_C
Cuts at: 3524 5501 6657 3524
Size: 1977 1156 4311
Fragments arranged by size:
4311 1977 1156
ApoI r'AATT_y
Cuts at: 1493 2582 3000 3233 3898 4185 4360 4753 4979
Size: 1089 418 233 665 287 175 393 226
Cuts at: 4979 5131 7240 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1089 665 418 393 287 233
226 175 152
AseI AT'TA_AT
Cuts at: 160 1032 5998 160
Size: 872 4966 1606
Fragments arranged by size:
4966 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
AvaI C'yCGr_G
Cuts at: 1870 1943 4769 1870
Size: 73 2826 4545
Fragments arranged by size:
4545 2826 73
BamHI G'GATC_C
Cuts at: 4774 5141 4774
Size: 367 7077
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3665 4295 4765 6220 618
Size: 325 217 627 1878 630 470 1455 1842
Fragments arranged by size:
1878 1842 1455 630 627 470 325 217
BanII G_rGCy'C
Cuts at: 1391 4763 1391
Size: 3372 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2756 3639 3877 4514 928
Size: 971 857 883 238 637 3858
Fragments arranged by size:
3858 971 883 857 637 238
BbvCI CC'TCA_GC
Cuts at: 4260 4260
Size: 7444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2616 2650 5635 5669 1903
Size: 34 679 34 2985 34 3678
Fragments arranged by size:
3678 2985 679 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4607 5336 6546 4607
Size: 729 1210 5505
Fragments arranged by size:
5505 1210 729
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7444
BfrBI ATG'CAT
Cuts at: 2721 4657 2721
Size: 1936 5508
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6055 136
Size: 107 122 71 631 605 4383 1525
Fragments arranged by size:
4383 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4560 4665 7439 4560
Size: 105 2774 4565
Fragments arranged by size:
4565 2774 105
BlpI GC'TnA_GC
Cuts at: 4861 4861
Size: 7444
Bme1580I G_kGCm'C
Cuts at: 1790 3528 4083 4308 5505 6661 1790
Size: 1738 555 225 1197 1156 2573
Fragments arranged by size:
2573 1738 1197 1156 555 225
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7444
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2064 4385 4644
Size: 416 53 83 399 24 647 2321 259
Cuts at: 4644 5175 6133 442
Size: 531 958 1753
Fragments arranged by size:
2321 1753 958 647 531 416 399 259
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4041 6104 1239
Size: 2802 2063 2579
Fragments arranged by size:
2802 2579 2063
Bpu10I CC'TnA_GC
Cuts at: 4260 4260
Size: 7444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3356 3500 3706 5569 6434 6732 3356
Size: 144 206 1863 865 298 4068
Fragments arranged by size:
4068 1863 865 298 206 144
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2329 4721 6107 882
Size: 617 830 2392 1386 2219
Fragments arranged by size:
2392 2219 1386 830 617
BsaAI yAC'GTr
Cuts at: 493 1204 2024 5235 493
Size: 711 820 3211 2702
Fragments arranged by size:
3211 2702 820 711
BsaBI GATnn'nnATC
Cuts at: 7438 7438
Size: 7444
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5251 5633 6268 275
Size: 53 83 186 936 3718 382 635 1451
Fragments arranged by size:
3718 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5877 6275 6549 6696 7107 5877
Size: 398 274 147 411 6214
Fragments arranged by size:
6214 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7219
Fragments arranged by size:
7219 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4739 1186
Size: 3553 3891
BseYI C'CCAG_C
Cuts at: 4701 6647 7143 4701
Size: 1946 496 5002
Fragments arranged by size:
5002 1946 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6871
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4894 5655 6683 1540
Size: 275 186 2893 761 1028 2301
Fragments arranged by size:
2893 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3507 3528 5505 5590 6661 3507
Size: 21 1977 85 1071 4290
Fragments arranged by size:
4290 1977 1071 85 21
BsmI GAATG_Cn'
Cuts at: 2078 2556 2929 3944 4702 4955 5048 2078
Size: 478 373 1015 758 253 93 4474
Fragments arranged by size:
4474 1015 758 478 373 253 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3507 3528 4083 4308 4763 5505 5590
Size: 399 1717 21 555 225 455 742 85
Cuts at: 5590 6661 1391
Size: 1071 2174
Fragments arranged by size:
2174 1717 1071 742 555 455 399 225
85 21
BspEI T'CCGG_A
Cuts at: 7107 7107
Size: 7444
BspHI T'CATG_A
Cuts at: 2724 3154 5333 7063 2724
Size: 430 2179 1730 3105
Fragments arranged by size:
3105 2179 1730 430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 4039 4799 844
Size: 1379 1816 760 3489
Fragments arranged by size:
3489 1816 1379 760
BsrBI CCG'CTC
Cuts at: 1394 1823 5331 1394
Size: 429 3508 3507
Fragments arranged by size:
3508 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3142 3339 3652 3664 5939 6113 65
Size: 1183 1894 197 313 12 2275 174 1396
Fragments arranged by size:
2275 1894 1396 1183 313 197 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 4476 6088 6275 1700
Size: 110 2666 1612 187 2869
Fragments arranged by size:
2869 2666 1612 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7444
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7444
BssSI C'ACGA_G
Cuts at: 5504 6516 5504
Size: 1012 6432
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4270 794
Size: 3476 3968
BstBI TT'CG_AA
Cuts at: 3733 6248 3733
Size: 2515 4929
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7444
BstXI CCAn_nnnn'nTGG
Cuts at: 3028 4019 3028
Size: 991 6453
BstYI r'GATC_y
Cuts at: 1972 4560 4665 4774 5141 5526 5543 6995 7081
Size: 2588 105 109 367 385 17 1452 86
Cuts at: 7081 7093 7439 1972
Size: 12 346 1977
Fragments arranged by size:
2588 1977 1452 385 367 346 109 105
86 17 12
BstZ17I GTA'TAC
Cuts at: 3928 3928
Size: 7444
BtgI C'CryG_G
Cuts at: 513 1065 2004 2887 2990 3130 513
Size: 552 939 883 103 140 4827
Fragments arranged by size:
4827 939 883 552 140 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6705
Fragments arranged by size:
6705 683 56
BtsI GCAGTG_nn'
Cuts at: 2097 2934 4232 4268 4960 5754 5774 2097
Size: 837 1298 36 692 794 20 3767
Fragments arranged by size:
3767 1298 837 794 692 36 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7444
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7409
DraI TTT'AAA
Cuts at: 3049 3639 3856 4748 3049
Size: 590 217 892 5745
Fragments arranged by size:
5745 892 590 217
DraIII CAC_nnn'GTG
Cuts at: 2838 2838
Size: 7444
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3617 4516 6451 809
Size: 507 2301 899 1935 1802
Fragments arranged by size:
2301 1935 1802 899 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4891 5780 7409
Size: 54 1425 50 275 18 3061 889 1629
Cuts at: 7409 8
Size: 43
Fragments arranged by size:
3061 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4891 1537
Size: 275 3079 4090
Fragments arranged by size:
4090 3079 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2534 2682 3468 3684 4668 4686 5374 1353
Size: 1181 148 786 216 984 18 688 3423
Fragments arranged by size:
3423 1181 984 786 688 216 148 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6031 6405 6551 387
Size: 1064 138 4442 374 146 1280
Fragments arranged by size:
4442 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 4041 4718 5507 6104 6891 7159
Size: 393 821 1588 677 789 597 787 268
Cuts at: 7159 1239
Size: 1524
Fragments arranged by size:
1588 1524 821 789 787 677 597 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 4736 4736
Size: 7444
EcoRI G'AATT_C
Cuts at: 2582 4185 4360 4753 5131 2582
Size: 1603 175 393 378 4895
Fragments arranged by size:
4895 1603 393 378 175
EcoRV GAT'ATC
Cuts at: 2061 3793 2061
Size: 1732 5712
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 7444
FspI TGC'GCA
Cuts at: 5950 5950
Size: 7444
HaeII r_GCGC'y
Cuts at: 1678 6591 1678
Size: 4913 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2402 2434 3809 3841 3905
Size: 32 135 32 830 32 1375 32 64
Cuts at: 3905 3937 4132 4164 6085 6117 6159 6191 1373
Size: 32 195 32 1921 32 42 32 2626
Fragments arranged by size:
2626 1921 1375 830 195 135 64 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4066 4788 5034 669
Size: 988 281 2128 722 246 3079
Fragments arranged by size:
3079 2128 988 722 281 246
HindIII A'AGCT_T
Cuts at: 748 3351 4279 748
Size: 2603 928 3913
Fragments arranged by size:
3913 2603 928
HpaI GTT'AAC
Cuts at: 5034 5034
Size: 7444
KpnI G_GTAC'C
Cuts at: 4769 4769
Size: 7444
MfeI C'AATT_G
Cuts at: 2282 2666 5043 2282
Size: 384 2377 4683
Fragments arranged by size:
4683 2377 384
MluI A'CGCG_T
Cuts at: 7320 7320
Size: 7444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3499 6558 6742 1305
Size: 2194 3059 184 2007
Fragments arranged by size:
3059 2194 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5622
Fragments arranged by size:
5622 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3135 3657 5402 5761 5920 518
Size: 648 1969 522 1745 359 159 2042
Fragments arranged by size:
2042 1969 1745 648 522 359 159
MspA1I CmG'CkG
Cuts at: 1606 3116 4860 5537 6685 6930 1606
Size: 1510 1744 677 1148 245 2120
Fragments arranged by size:
2120 1744 1510 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2990 3130 513
Size: 552 939 986 140 4827
Fragments arranged by size:
4827 986 939 552 140
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7444
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7444
NotI GC'GGCC_GC
Cuts at: 4891 4891
Size: 7444
NsiI A_TGCA'T
Cuts at: 2723 4659 2723
Size: 1936 5508
NspI r_CATG'y
Cuts at: 1132 1229 4657 4802 6347 1132
Size: 97 3428 145 1545 2229
Fragments arranged by size:
3428 2229 1545 145 97
PasI CC'CwG_GG
Cuts at: 1348 4300 1348
Size: 2952 4492
PciI A'CATG_T
Cuts at: 6343 6343
Size: 7444
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3809 1108
Size: 2701 4743
PfoI T'CCnGG_A
Cuts at: 4599 4599
Size: 7444
PmeI GTTT'AAAC
Cuts at: 4748 4748
Size: 7444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5513 5545 7026 7058 5513
Size: 32 1481 32 5899
Fragments arranged by size:
5899 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2462 2760 1613
Size: 849 298 6297
Fragments arranged by size:
6297 849 298
PsiI TTA'TAA
Cuts at: 5014 5014
Size: 7444
PstI C_TGCA'G
Cuts at: 830 3121 4796 830
Size: 2291 1675 3478
Fragments arranged by size:
3478 2291 1675
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7444
PvuII CAG'CTG
Cuts at: 3116 3116
Size: 7444
SalI G'TCGA_C
Cuts at: 1936 4786 1936
Size: 2850 4594
SapI GCTCTTCn'nnn_
Cuts at: 3468 3468
Size: 7444
SbfI CC_TGCA'GG
Cuts at: 4796 4796
Size: 7444
ScaI AGT'ACT
Cuts at: 5692 7404 5692
Size: 1712 5732
SexAI A'CCwGG_T
Cuts at: 2338 2338
Size: 7444
SfcI C'TryA_G
Cuts at: 826 1045 2194 2604 3117 3574 4343 4792 5927
Size: 219 1149 410 513 457 769 449 1135
Cuts at: 5927 6608 6799 826
Size: 681 191 1471
Fragments arranged by size:
1471 1149 1135 769 681 513 457 449
410 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7444
SmaI CCC'GGG
Cuts at: 1872 4771 1872
Size: 2899 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3371 3479 3685 5548 6449 6711
Size: 197 926 1428 108 206 1863 901 262
Cuts at: 6711 820
Size: 1553
Fragments arranged by size:
1863 1553 1428 926 901 262 206 197
108
SnaBI TAC'GTA
Cuts at: 493 5235 493
Size: 4742 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7444
SphI G_CATG'C
Cuts at: 1132 4802 1132
Size: 3670 3774
SspI AAT'ATT
Cuts at: 5 52 2170 3635 5368 5
Size: 47 2118 1465 1733 2081
Fragments arranged by size:
2118 2081 1733 1465 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7444
StyI C'CwwG_G
Cuts at: 513 1065 2004 2622 2990 3130 3889 4434 4883
Size: 552 939 618 368 140 759 545 449
Cuts at: 4883 513
Size: 3074
Fragments arranged by size:
3074 939 759 618 552 545 449 368
140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4313 5471 5488 5641 5826 6271 325
Size: 1255 2733 1158 17 153 185 445 1498
Fragments arranged by size:
2733 1498 1255 1158 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5690 7402 96
Size: 274 80 33 51 1443 3713 1712 138
Fragments arranged by size:
3713 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2725 2757 3497 3529 7404 7436 2725
Size: 32 740 32 3875 32 2733
Fragments arranged by size:
3875 2733 740 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7444
XbaI T'CTAG_A
Cuts at: 4780 4780
Size: 7444
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3793 3793
Size: 7444
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7444
XmaI C'CCGG_G
Cuts at: 1870 4769 1870
Size: 2899 4545
XmnI GAAnn'nnTTC
Cuts at: 2291 2868 3898 5573 2291
Size: 577 1030 1675 4162
Fragments arranged by size:
4162 1675 1030 577
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5252 276
Size: 53 83 186 936 3718 2468
Fragments arranged by size:
3718 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI Bsu36I EcoICRI EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI