Restriction map for
pFN21AE3316
(Circular) (Six-base) MAPSORT of: pFN21AE3316.seq Check: 800 from: 1 to: 7339
pFN21AE3316 7339 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4360 4360
Size: 7339
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5149 278
Size: 53 83 186 936 3613 2468
Fragments arranged by size:
3613 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2741 3648 4682 1937
Size: 804 907 1034 4594
Fragments arranged by size:
4594 1034 907 804
Acc65I G'GTAC_C
Cuts at: 4660 4660
Size: 7339
AclI AA'CG_TT
Cuts at: 1563 5466 5839 1563
Size: 3903 373 3063
Fragments arranged by size:
3903 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2533 2682 3352 3527 5402 6786 7054 1632
Size: 901 149 670 175 1875 1384 268 1917
Fragments arranged by size:
1917 1875 1384 901 670 268 175 149
AfeI AGC'GCT
Cuts at: 2540 2540
Size: 7339
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7142
AflIII A'CryG_T
Cuts at: 1203 3903 4041 4203 6238 7215 1203
Size: 2700 138 162 2035 977 1327
Fragments arranged by size:
2700 2035 1327 977 162 138
AgeI A'CCGG_T
Cuts at: 6170 6170
Size: 7339
AhdI GACnn_n'nnGTC
Cuts at: 2305 4207 6068 2305
Size: 1902 1861 3576
Fragments arranged by size:
3576 1902 1861
AleI CACnn'nnGTG
Cuts at: 1166 3087 1166
Size: 1921 5418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3215 1184
Size: 2031 5308
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2228 2260 3836 3868 2228
Size: 32 1576 32 5699
Fragments arranged by size:
5699 1576 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2881 3526 3817 3979 4320 4615 5082 6654 2881
Size: 645 291 162 341 295 467 1572 3566
Fragments arranged by size:
3566 1572 645 467 341 295 291 162
ApaI G_GGCC'C
Cuts at: 2578 2578
Size: 7339
ApaLI G'TGCA_C
Cuts at: 2432 5396 6552 2432
Size: 2964 1156 3219
Fragments arranged by size:
3219 2964 1156
ApoI r'AATT_y
Cuts at: 1493 3324 4648 4874 5026 7135 1493
Size: 1831 1324 226 152 2109 1697
Fragments arranged by size:
2109 1831 1697 1324 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5893 160
Size: 872 4861 1606
Fragments arranged by size:
4861 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7339
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 2125 3495 3827 4163 4664 1870
Size: 73 105 77 1370 332 336 501 4545
Fragments arranged by size:
4545 1370 501 336 332 105 77 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4415 4448 4415
Size: 33 7306
BamHI G'GATC_C
Cuts at: 4669 5036 4669
Size: 367 6972
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2446 2586 2633 2791 3261
Size: 325 217 627 659 140 47 158 470
Cuts at: 3261 3386 4159 4244 4660 6115 618
Size: 125 773 85 416 1455 1842
Fragments arranged by size:
1842 1455 773 659 627 470 416 325
217 158 140 125 85 47
BanII G_rGCy'C
Cuts at: 1391 2037 2578 3128 3680 4658 1391
Size: 646 541 550 552 978 4072
Fragments arranged by size:
4072 978 646 552 550 541
BbeI G_GCGC'C
Cuts at: 2450 2590 3265 2450
Size: 140 675 6524
Fragments arranged by size:
6524 675 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3725 928
Size: 971 1826 4542
Fragments arranged by size:
4542 1826 971
BbvCI CC'TCA_GC
Cuts at: 3486 4238 3486
Size: 752 6587
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2423 2457 5530 5564 1903
Size: 34 486 34 3073 34 3678
Fragments arranged by size:
3678 3073 486 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5231 6441 5231
Size: 1210 6129
BclI T'GATC_A
Cuts at: 1548 2461 4221 1548
Size: 913 1760 4666
Fragments arranged by size:
4666 1760 913
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3475 4157 4242
Size: 107 122 71 631 605 1803 682 85
Cuts at: 4242 5950 136
Size: 1708 1525
Fragments arranged by size:
1803 1708 1525 682 631 605 122 107
85 71
BglII A'GATC_T
Cuts at: 3654 7334 3654
Size: 3680 3659
BlpI GC'TnA_GC
Cuts at: 2669 4756 2669
Size: 2087 5252
Bme1580I G_kGCm'C
Cuts at: 1790 2436 2578 3389 4145 5400 6556 1790
Size: 646 142 811 756 1255 1156 2573
Fragments arranged by size:
2573 1255 1156 811 756 646 142
BmgBI CAC'GTC
Cuts at: 1318 2307 2601 3733 4440 1318
Size: 989 294 1132 707 4217
Fragments arranged by size:
4217 1132 989 707 294
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5070 6028 442
Size: 416 53 83 399 24 3653 958 1753
Fragments arranged by size:
3653 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3863 4342 4359 4481 5999 1239
Size: 2624 479 17 122 1518 2579
Fragments arranged by size:
2624 2579 1518 479 122 17
Bpu10I CC'TnA_GC
Cuts at: 3486 3679 3945 4238 3486
Size: 193 266 293 6587
Fragments arranged by size:
6587 293 266 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2489 2843 5464 6329 6627 2489
Size: 354 2621 865 298 3201
Fragments arranged by size:
3201 2621 865 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2064 2143 6002 882
Size: 617 565 79 3859 2219
Fragments arranged by size:
3859 2219 617 565 79
BsaAI yAC'GTr
Cuts at: 493 1204 5130 493
Size: 711 3926 2702
Fragments arranged by size:
3926 2702 711
BsaBI GATnn'nnATC
Cuts at: 7333 7333
Size: 7339
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2447 2587 3262 3860
Size: 53 83 186 936 914 140 675 598
Cuts at: 3860 5146 5528 6163 275
Size: 1286 382 635 1451
Fragments arranged by size:
1451 1286 936 914 675 635 598 382
186 140 83 53
BsaWI w'CCGG_w
Cuts at: 2339 3044 3714 3789 5772 6170 6444 6591 7002
Size: 705 670 75 1983 398 274 147 411
Cuts at: 7002 2339
Size: 2676
Fragments arranged by size:
2676 1983 705 670 411 398 274 147
75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3836 3866 1178
Size: 30 165 30 2433 30 4651
Fragments arranged by size:
4651 2433 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2052 2713 2846 2999 3107 3110 1186
Size: 866 661 133 153 108 3 5415
Fragments arranged by size:
5415 866 661 153 133 108 3
BseYI C'CCAG_C
Cuts at: 3501 3554 3673 4512 6542 7038 3501
Size: 53 119 839 2030 496 3802
Fragments arranged by size:
3802 2030 839 496 119 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2638 1163
Size: 573 902 5864
Fragments arranged by size:
5864 902 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4789 5550 6578 1540
Size: 275 186 2788 761 1028 2301
Fragments arranged by size:
2788 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2436 3128 5400 5485 6556 2436
Size: 692 2272 85 1071 3219
Fragments arranged by size:
3219 2272 1071 692 85
BsmI GAATG_Cn'
Cuts at: 3079 4850 4943 3079
Size: 1771 93 5475
Fragments arranged by size:
5475 1771 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2607 3851 2607
Size: 1244 6095
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2436 2578 3128 3389 3680 4145
Size: 399 247 399 142 550 261 291 465
Cuts at: 4145 4658 5400 5485 6556 1391
Size: 513 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 550 513 465 399 399
291 261 247 142 85
BspEI T'CCGG_A
Cuts at: 2339 3789 7002 2339
Size: 1450 3213 2676
Fragments arranged by size:
3213 2676 1450
BspHI T'CATG_A
Cuts at: 5228 6958 5228
Size: 1730 5609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4360 4371 4694 844
Size: 3516 11 323 3489
Fragments arranged by size:
3516 3489 323 11
BsrBI CCG'CTC
Cuts at: 1394 1823 4131 4194 5226 1394
Size: 429 2308 63 1032 3507
Fragments arranged by size:
3507 2308 1032 429 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 2891 3636 5834 6008 65
Size: 1183 1643 745 2198 174 1396
Fragments arranged by size:
2198 1643 1396 1183 745 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3936 4147 5983 6170 1700
Size: 110 2126 211 1836 187 2869
Fragments arranged by size:
2869 2126 1836 211 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7339
BssHII G'CGCG_C
Cuts at: 1925 4508 1925
Size: 2583 4756
BssSI C'ACGA_G
Cuts at: 5399 6411 5399
Size: 1012 6327
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2329 2881 2895 3166 3817 4540 794
Size: 1535 552 14 271 651 723 3593
Fragments arranged by size:
3593 1535 723 651 552 271 14
BstBI TT'CG_AA
Cuts at: 6143 6143
Size: 7339
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7339
BstXI CCAn_nnnn'nTGG
Cuts at: 2605 2761 2605
Size: 156 7183
BstYI r'GATC_y
Cuts at: 1972 2115 2280 2409 3618 3654 3975 4284 4570
Size: 143 165 129 1209 36 321 309 286
Cuts at: 4570 4669 5036 5421 5438 6890 6976 6988 7334
Size: 99 367 385 17 1452 86 12 346
Cuts at: 7334 1972
Size: 1977
Fragments arranged by size:
1977 1452 1209 385 367 346 321 309
286 165 143 129 99 86 36 17
12
BstZ17I GTA'TAC
Cuts at: 2742 2742
Size: 7339
Bsu36I CC'TnA_GG
Cuts at: 4071 4071
Size: 7339
BtgI C'CryG_G
Cuts at: 513 1065 2004 4560 513
Size: 552 939 2556 3292
Fragments arranged by size:
3292 2556 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2526 487
Size: 683 56 1300 5300
Fragments arranged by size:
5300 1300 683 56
BtsI GCAGTG_nn'
Cuts at: 2464 3397 3414 4855 5649 5669 2464
Size: 933 17 1441 794 20 4134
Fragments arranged by size:
4134 1441 933 794 20 17
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7339
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2551 2586 529
Size: 35 1987 35 5282
Fragments arranged by size:
5282 1987 35 35
DraI TTT'AAA
Cuts at: 4643 4643
Size: 7339
DraIII CAC_nnn'GTG
Cuts at: 2623 2623
Size: 7339
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3047 6346 809
Size: 507 1731 3299 1802
Fragments arranged by size:
3299 1802 1731 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2310 2388
Size: 54 1425 50 275 18 177 303 78
Cuts at: 2388 2607 2614 3801 4311 4786 5675 7304 8
Size: 219 7 1187 510 475 889 1629 43
Fragments arranged by size:
1629 1425 1187 889 510 475 303 275
219 177 78 54 50 43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 4786 1537
Size: 275 2974 4090
Fragments arranged by size:
4090 2974 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2486 2658 2722 2731 2873 3293 5269 1353
Size: 1133 172 64 9 142 420 1976 3423
Fragments arranged by size:
3423 1976 1133 420 172 142 64 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2055 2402 3346 3506 4115 4634
Size: 1064 138 466 347 944 160 609 519
Cuts at: 4634 5926 6300 6446 387
Size: 1292 374 146 1280
Fragments arranged by size:
1292 1280 1064 944 609 519 466 374
347 160 146 138
EcoICRI GAG'CTC
Cuts at: 3126 3126
Size: 7339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2533 2682 3352 3527 3863 4342 4359
Size: 393 901 149 670 175 336 479 17
Cuts at: 4359 4481 5402 5999 6786 7054 1239
Size: 122 921 597 787 268 1524
Fragments arranged by size:
1524 921 901 787 670 597 479 393
336 268 175 149 122 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 2951 4367 2366
Size: 585 1416 5338
Fragments arranged by size:
5338 1416 585
EcoO109I rG'GnC_Cy
Cuts at: 2500 2574 2863 2878 2956 3523 4581 2500
Size: 74 289 15 78 567 1058 5258
Fragments arranged by size:
5258 1058 567 289 78 74 15
EcoRI G'AATT_C
Cuts at: 4648 5026 4648
Size: 378 6961
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 7339
FspI TGC'GCA
Cuts at: 4382 5845 4382
Size: 1463 5876
HaeII r_GCGC'y
Cuts at: 1678 2450 2542 2590 3021 3265 3561 3952 6486
Size: 772 92 48 431 244 296 391 2534
Cuts at: 6486 1678
Size: 2531
Fragments arranged by size:
2534 2531 772 431 391 296 244 92
48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 2291 2323 4193
Size: 32 135 32 503 32 184 32 1870
Cuts at: 4193 4225 5980 6012 6054 6086 1373
Size: 32 1755 32 42 32 2626
Fragments arranged by size:
2626 1870 1755 503 184 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4683 4929 669
Size: 988 281 2745 246 3079
Fragments arranged by size:
3079 2745 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3585 748
Size: 2837 4502
HpaI GTT'AAC
Cuts at: 4929 4929
Size: 7339
KasI G'GCGC_C
Cuts at: 2446 2586 3261 2446
Size: 140 675 6524
Fragments arranged by size:
6524 675 140
KpnI G_GTAC'C
Cuts at: 4664 4664
Size: 7339
MfeI C'AATT_G
Cuts at: 4938 4938
Size: 7339
MluI A'CGCG_T
Cuts at: 7215 7215
Size: 7339
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3633 3888 6453 6637 1305
Size: 2328 255 2565 184 2007
Fragments arranged by size:
2565 2328 2007 255 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2609 2616 3803 10
Size: 54 1425 343 177 600 7 1187 3546
Fragments arranged by size:
3546 1425 1187 600 343 177 54 7
MslI CAynn'nnrTG
Cuts at: 518 1166 3087 3602 4497 5297 5656 5815 518
Size: 648 1921 515 895 800 359 159 2042
Fragments arranged by size:
2042 1921 895 800 648 515 359 159
MspA1I CmG'CkG
Cuts at: 1606 2218 2326 3331 4242 4337 4540 4755 5432
Size: 612 108 1005 911 95 203 215 677
Cuts at: 5432 6580 6825 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1005 911 677 612 245 215
203 108 95
NaeI GCC'GGC
Cuts at: 3938 4149 3938
Size: 211 7128
NarI GG'CG_CC
Cuts at: 2447 2587 3262 2447
Size: 140 675 6524
Fragments arranged by size:
6524 675 140
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5848
Fragments arranged by size:
5848 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7339
NgoMIV G'CCGG_C
Cuts at: 3936 4147 3936
Size: 211 7128
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7339
NotI GC'GGCC_GC
Cuts at: 4786 4786
Size: 7339
NspI r_CATG'y
Cuts at: 1132 1229 3853 3907 4207 4609 4697 6242 1132
Size: 97 2624 54 300 402 88 1545 2229
Fragments arranged by size:
2624 2229 1545 402 300 97 88 54
PasI CC'CwG_GG
Cuts at: 1348 3130 1348
Size: 1782 5557
PciI A'CATG_T
Cuts at: 3903 4203 6238 3903
Size: 300 2035 5004
Fragments arranged by size:
5004 2035 300
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3979 4320 1108
Size: 2871 341 4127
Fragments arranged by size:
4127 2871 341
PfoI T'CCnGG_A
Cuts at: 2349 2349
Size: 7339
PmeI GTTT'AAAC
Cuts at: 4643 4643
Size: 7339
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 4106 4138 5408 5440 6921 6953 3836
Size: 32 238 32 1270 32 1481 32 4222
Fragments arranged by size:
4222 1481 1270 238 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7339
PsiI TTA'TAA
Cuts at: 4909 4909
Size: 7339
PspOMI G'GGCC_C
Cuts at: 2574 2574
Size: 7339
PstI C_TGCA'G
Cuts at: 830 2091 2325 3813 4371 4691 830
Size: 1261 234 1488 558 320 3478
Fragments arranged by size:
3478 1488 1261 558 320 234
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7339
PvuII CAG'CTG
Cuts at: 2218 2326 4337 2218
Size: 108 2011 5220
Fragments arranged by size:
5220 2011 108
SacI G_AGCT'C
Cuts at: 3128 3128
Size: 7339
SalI G'TCGA_C
Cuts at: 1936 4681 1936
Size: 2745 4594
SapI GCTCTTCn'nnn_
Cuts at: 2486 2658 3293 2486
Size: 172 635 6532
Fragments arranged by size:
6532 635 172
SbfI CC_TGCA'GG
Cuts at: 4691 4691
Size: 7339
ScaI AGT'ACT
Cuts at: 5587 7299 5587
Size: 1712 5627
SexAI A'CCwGG_T
Cuts at: 2361 2361
Size: 7339
SfcI C'TryA_G
Cuts at: 826 1045 2087 2321 2566 3809 4367 4390 4687
Size: 219 1042 234 245 1243 558 23 297
Cuts at: 4687 5822 6503 6694 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1243 1135 1042 681 558 297 245
234 219 191 23
SfoI GGC'GCC
Cuts at: 2448 2588 3263 2448
Size: 140 675 6524
Fragments arranged by size:
6524 675 140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7339
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7339
SmaI CCC'GGG
Cuts at: 1872 2050 2127 3497 3829 4666 1872
Size: 178 77 1370 332 837 4545
Fragments arranged by size:
4545 1370 837 332 178 77
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2504 2858 5443 6344 6606 820
Size: 197 926 561 354 2585 901 262 1553
Fragments arranged by size:
2585 1553 926 901 561 354 262 197
SnaBI TAC'GTA
Cuts at: 493 5130 493
Size: 4637 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7339
SphI G_CATG'C
Cuts at: 1132 4697 1132
Size: 3565 3774
SspI AAT'ATT
Cuts at: 5 52 5263 5
Size: 47 5211 2081
Fragments arranged by size:
5211 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7339
StyI C'CwwG_G
Cuts at: 513 1065 2004 2781 4778 513
Size: 552 939 777 1997 3074
Fragments arranged by size:
3074 1997 939 777 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3202 3568 3986 5366 5383 5536 5721
Size: 1255 1622 366 418 1380 17 153 185
Cuts at: 5721 6166 325
Size: 445 1498
Fragments arranged by size:
1622 1498 1380 1255 445 418 366 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5585 7297 96
Size: 274 80 33 51 1443 3608 1712 138
Fragments arranged by size:
3608 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3260 3292 3448 3480 7299 7331 3260
Size: 32 156 32 3819 32 3268
Fragments arranged by size:
3819 3268 156 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7339
XbaI T'CTAG_A
Cuts at: 4675 4675
Size: 7339
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3433 4334 3433
Size: 901 6438
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7339
XmaI C'CCGG_G
Cuts at: 1870 2048 2125 3495 3827 4664 1870
Size: 178 77 1370 332 837 4545
Fragments arranged by size:
4545 1370 837 332 178 77
XmnI GAAnn'nnTTC
Cuts at: 4121 5468 4121
Size: 1347 5992
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5147 276
Size: 53 83 186 936 3613 2468
Fragments arranged by size:
3613 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BplI BsiWI EcoRV FseI FspAI
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SfiI SrfI SwaI